Restriction map for
pF1KA2004
(Circular) (Six-base) MAPSORT of: pF1KA2004.seq Check: 159 from: 1 to: 7880
pF1KA2004 7880 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 648 3485 4918 648
Size: 2837 1433 3610
Fragments arranged by size:
3610 2837 1433
Acc65I G'GTAC_C
Cuts at: 4896 4896
Size: 7880
AclI AA'CG_TT
Cuts at: 7720 7720
Size: 7880
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 761 1420 5705 6137 6490 7152 761
Size: 659 4285 432 353 662 1489
Fragments arranged by size:
4285 1489 662 659 432 353
AflIII A'CryG_T
Cuts at: 586 5232 7032 7313 586
Size: 4646 1800 281 1153
Fragments arranged by size:
4646 1800 1153 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 969 3375 4556 969
Size: 2406 1181 4293
Fragments arranged by size:
4293 2406 1181
AlwNI CAG_nnn'CTG
Cuts at: 5185 6623 5185
Size: 1438 6442
ApaLI G'TGCA_C
Cuts at: 1840 6718 1840
Size: 4878 3002
ApoI r'AATT_y
Cuts at: 209 610 694 748 789 1120 1243 1907 2119
Size: 401 84 54 41 331 123 664 212
Cuts at: 2119 2128 2377 2926 2971 3150 3184 3734 3889
Size: 9 249 549 45 179 34 550 155
Cuts at: 3889 4266 4491 4884 7233 7489 209
Size: 377 225 393 2349 256 600
Fragments arranged by size:
2349 664 600 550 549 401 393 377
331 256 249 225 212 179 155 123
84 54 45 41 34 9
AseI AT'TA_AT
Cuts at: 20 1888 4631 20
Size: 1868 2743 3269
Fragments arranged by size:
3269 2743 1868
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7880
AvaI C'yCGr_G
Cuts at: 4900 4900
Size: 7880
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 575 608 575
Size: 33 7847
BamHI G'GATC_C
Cuts at: 2645 3828 4905 2645
Size: 1183 1077 5620
Fragments arranged by size:
5620 1183 1077
BanI G'GyrC_C
Cuts at: 2989 4896 5310 5558 5593 2989
Size: 1907 414 248 35 5276
Fragments arranged by size:
5276 1907 414 248 35
BanII G_rGCy'C
Cuts at: 533 4148 4894 5924 533
Size: 3615 746 1030 2489
Fragments arranged by size:
3615 2489 1030 746
BbeI G_GCGC'C
Cuts at: 5314 5562 5314
Size: 248 7632
BbsI GAAGACnn'nnnn_
Cuts at: 932 1706 2156 2984 4624 932
Size: 774 450 828 1640 4188
Fragments arranged by size:
4188 1640 828 774 450
BciVI GTATCCnnnnn_n'
Cuts at: 3340 3662 4309 5778 6834 7880 3340
Size: 322 647 1469 1056 1046 3340
Fragments arranged by size:
3340 1469 1056 1046 647 322
BclI T'GATC_A
Cuts at: 2765 5400 2765
Size: 2635 5245
BfrBI ATG'CAT
Cuts at: 3352 4521 3352
Size: 1169 6711
BglII A'GATC_T
Cuts at: 1869 5395 7432 1869
Size: 3526 2037 2317
Fragments arranged by size:
3526 2317 2037
BlpI GC'TnA_GC
Cuts at: 4992 4992
Size: 7880
Bme1580I G_kGCm'C
Cuts at: 1844 2992 5505 5598 6722 1844
Size: 1148 2513 93 1124 3002
Fragments arranged by size:
3002 2513 1148 1124 93
BmgBI CAC'GTC
Cuts at: 228 228
Size: 7880
BmrI ACTGGGnnnn_n'
Cuts at: 374 3429 3484 5092 5173 5508 7652 374
Size: 3055 55 1608 81 335 2144 602
Fragments arranged by size:
3055 2144 1608 602 335 81 55
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1789 6247 1789
Size: 4458 3422
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3724 4296 3724
Size: 572 7308
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3570 4463 5056 5935 6649 6947 3570
Size: 893 593 879 714 298 4503
Fragments arranged by size:
4503 893 879 714 593 298
BsaI GGTCTCn'nnnn_
Cuts at: 7584 7584
Size: 7880
BsaAI yAC'GTr
Cuts at: 796 2481 5233 796
Size: 1685 2752 3443
Fragments arranged by size:
3443 2752 1685
BsaBI GATnn'nnATC
Cuts at: 6 164 5394 7431 6
Size: 158 5230 2037 455
Fragments arranged by size:
5230 2037 455 158
BsaHI Gr'CG_yC
Cuts at: 5311 5559 6261 7757 5311
Size: 248 702 1496 5434
Fragments arranged by size:
5434 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2810 5069 5590 6268 6679 6826 7100 2810
Size: 2259 521 678 411 147 274 3590
Fragments arranged by size:
3590 2259 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2420 2450 2420
Size: 30 7850
BseYI C'CCAG_C
Cuts at: 1573 5305 6063 6728 7136 1573
Size: 3732 758 665 408 2317
Fragments arranged by size:
3732 2317 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2291 2291
Size: 7880
BsiEI CG_ry'CG
Cuts at: 104 5422 5468 6698 104
Size: 5318 46 1230 1286
Fragments arranged by size:
5318 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 533 1844 4894 5672 5862 6722 533
Size: 1311 3050 778 190 860 1691
Fragments arranged by size:
3050 1691 1311 860 778 190
BsmI GAATG_Cn'
Cuts at: 956 1057 3352 3592 956
Size: 101 2295 240 5244
Fragments arranged by size:
5244 2295 240 101
BsmBI CGTCTCn'nnnn_
Cuts at: 1534 1534
Size: 7880
Bsp1286I G_dGCh'C
Cuts at: 533 1844 2992 4148 4894 5505 5598 5672 5862
Size: 1311 1148 1156 746 611 93 74 190
Cuts at: 5862 5924 6722 533
Size: 62 798 1691
Fragments arranged by size:
1691 1311 1156 1148 798 746 611 190
93 74 62
BspEI T'CCGG_A
Cuts at: 2810 2810
Size: 7880
BspHI T'CATG_A
Cuts at: 2051 2768 6312 2051
Size: 717 3544 3619
Fragments arranged by size:
3619 3544 717
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1586 4930 5144 5446 5827 1586
Size: 3344 214 302 381 3639
Fragments arranged by size:
3639 3344 381 302 214
BsrBI CCG'CTC
Cuts at: 50 6172 6226 7710 7875 50
Size: 6122 54 1484 165 55
Fragments arranged by size:
6122 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 494 762 1596 2261 2468 3414 5792 494
Size: 268 834 665 207 946 2378 2582
Fragments arranged by size:
2582 2378 946 834 665 268 207
BsrFI r'CCGG_y
Cuts at: 5878 6059 5878
Size: 181 7699
BssSI C'ACGA_G
Cuts at: 6151 6859 6151
Size: 708 7172
BstAPI GCAn_nnn'nTGC
Cuts at: 3198 3580 5257 3198
Size: 382 1677 5821
Fragments arranged by size:
5821 1677 382
BstBI TT'CG_AA
Cuts at: 6241 6241
Size: 7880
BstEII G'GTnAC_C
Cuts at: 2436 2436
Size: 7880
BstXI CCAn_nnnn'nTGG
Cuts at: 1643 1643
Size: 7880
BstYI r'GATC_y
Cuts at: 1 1767 1803 1869 2645 3828 4905 5372 5395
Size: 1766 36 66 776 1183 1077 467 23
Cuts at: 5395 5730 5976 6282 6294 6380 7432 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1766 1183 1077 1052 776 467 449 335
306 246 86 66 36 23 12
BtgI C'CryG_G
Cuts at: 107 837 107
Size: 730 7150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3242 5830 6011 6174 3242
Size: 2588 181 163 4948
Fragments arranged by size:
4948 2588 181 163
BtsI GCAGTG_nn'
Cuts at: 5123 5123
Size: 7880
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 956 991 956
Size: 35 7845
DraI TTT'AAA
Cuts at: 1194 1206 4124 4879 1194
Size: 12 2918 755 4195
Fragments arranged by size:
4195 2918 755 12
DraIII CAC_nnn'GTG
Cuts at: 2920 2920
Size: 7880
DrdI GACnn_nn'nnGTC
Cuts at: 934 5586 6930 934
Size: 4652 1344 1884
Fragments arranged by size:
4652 1884 1344
EaeI y'GGCC_r
Cuts at: 4277 4426 5465 5639 6030 6057 7402 4277
Size: 149 1039 174 391 27 1345 4755
Fragments arranged by size:
4755 1345 1039 391 174 149 27
EagI C'GGCC_G
Cuts at: 5465 5465
Size: 7880
EarI CTCTTCn'nnn_
Cuts at: 725 1937 2202 2879 3965 5903 6113 725
Size: 1212 265 677 1086 1938 210 2492
Fragments arranged by size:
2492 1938 1212 1086 677 265 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6088 6830 6976 7690 6088
Size: 742 146 714 6278
Fragments arranged by size:
6278 742 714 146
EcoICRI GAG'CTC
Cuts at: 531 4892 531
Size: 4361 3519
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 761 1420 3724 4296 5705 6137 6490 7152 761
Size: 659 2304 572 1409 432 353 662 1489
Fragments arranged by size:
2304 1489 1409 662 659 572 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 659 659
Size: 7880
EcoO109I rG'GnC_Cy
Cuts at: 5019 5019
Size: 7880
EcoRI G'AATT_C
Cuts at: 610 694 789 2128 4884 610
Size: 84 95 1339 2756 3606
Fragments arranged by size:
3606 2756 1339 95 84
EcoRV GAT'ATC
Cuts at: 2049 2049
Size: 7880
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 283 1891 2228 3417 3736 4366 5899 283
Size: 1608 337 1189 319 630 1533 2264
Fragments arranged by size:
2264 1608 1533 1189 630 337 319
FspI TGC'GCA
Cuts at: 5661 5661
Size: 7880
HaeII r_GCGC'y
Cuts at: 5314 5562 6792 7563 5314
Size: 248 1230 771 5631
Fragments arranged by size:
5631 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1293 1325 1426 1458 1536 1568 3038 3070 3776
Size: 32 101 32 78 32 1470 32 706
Cuts at: 3776 3808 4801 4833 5722 5752 5754 5784 1293
Size: 32 993 32 889 30 2 30 3389
Fragments arranged by size:
3389 1470 993 889 706 101 78 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 4786 4919 4786
Size: 133 7747
HindIII A'AGCT_T
Cuts at: 1466 1466
Size: 7880
KasI G'GCGC_C
Cuts at: 5310 5558 5310
Size: 248 7632
KpnI G_GTAC'C
Cuts at: 4900 4900
Size: 7880
MfeI C'AATT_G
Cuts at: 4538 4716 4538
Size: 178 7702
MluI A'CGCG_T
Cuts at: 7313 7313
Size: 7880
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 617 963 3113 4326 6639 6823 617
Size: 346 2150 1213 2313 184 1674
Fragments arranged by size:
2313 2150 1674 1213 346 184
MscI TGG'CCA
Cuts at: 4279 4428 5641 4279
Size: 149 1213 6518
Fragments arranged by size:
6518 1213 149
MslI CAynn'nnrTG
Cuts at: 1901 3227 3239 3287 5869 5996 1901
Size: 1326 12 48 2582 127 3785
Fragments arranged by size:
3785 2582 1326 127 48 12
MspA1I CmG'CkG
Cuts at: 1577 4991 5305 5665 6449 6694 1577
Size: 3414 314 360 784 245 2763
Fragments arranged by size:
3414 2763 784 360 314 245
NaeI GCC'GGC
Cuts at: 6061 6061
Size: 7880
NarI GG'CG_CC
Cuts at: 5311 5559 5311
Size: 248 7632
NcoI C'CATG_G
Cuts at: 107 837 107
Size: 730 7150
NdeI CA'TA_TG
Cuts at: 2886 4517 2886
Size: 1631 6249
NgoMIV G'CCGG_C
Cuts at: 6059 6059
Size: 7880
NsiI A_TGCA'T
Cuts at: 3354 4523 3354
Size: 1169 6711
NspI r_CATG'y
Cuts at: 590 3583 4933 5433 7036 590
Size: 2993 1350 500 1603 1434
Fragments arranged by size:
2993 1603 1434 1350 500
PciI A'CATG_T
Cuts at: 586 7032 586
Size: 6446 1434
PflMI CCAn_nnn'nTGG
Cuts at: 2011 2329 3369 2011
Size: 318 1040 6522
Fragments arranged by size:
6522 1040 318
PmeI GTTT'AAAC
Cuts at: 4879 4879
Size: 7880
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3139 3171 6326 6358 3139
Size: 32 3155 32 4661
Fragments arranged by size:
4661 3155 32 32
PstI C_TGCA'G
Cuts at: 2540 4927 5612 2540
Size: 2387 685 4808
Fragments arranged by size:
4808 2387 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7880
PvuII CAG'CTG
Cuts at: 1577 5305 5665 1577
Size: 3728 360 3792
Fragments arranged by size:
3792 3728 360
RsrII CG'GwC_CG
Cuts at: 6075 6075
Size: 7880
SacI G_AGCT'C
Cuts at: 533 4894 533
Size: 4361 3519
SalI G'TCGA_C
Cuts at: 4917 4917
Size: 7880
SapI GCTCTTCn'nnn_
Cuts at: 2879 5903 6113 2879
Size: 3024 210 4646
Fragments arranged by size:
4646 3024 210
SbfI CC_TGCA'GG
Cuts at: 4927 4927
Size: 7880
ScaI AGT'ACT
Cuts at: 999 2542 7397 999
Size: 1543 4855 1482
Fragments arranged by size:
4855 1543 1482
SfcI C'TryA_G
Cuts at: 33 649 2536 2558 4923 5608 6576 6767 33
Size: 616 1887 22 2365 685 968 191 1146
Fragments arranged by size:
2365 1887 1146 968 685 616 191 22
SfoI GGC'GCC
Cuts at: 5312 5560 5312
Size: 248 7632
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7880
SmaI CCC'GGG
Cuts at: 4902 4902
Size: 7880
SmlI C'TyrA_G
Cuts at: 3585 4478 5035 5950 6664 6926 3585
Size: 893 557 915 714 262 4539
Fragments arranged by size:
4539 915 893 714 557 262
SnaBI TAC'GTA
Cuts at: 2481 2481
Size: 7880
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7880
SphI G_CATG'C
Cuts at: 4933 5433 4933
Size: 500 7380
SspI AAT'ATT
Cuts at: 2835 3957 2835
Size: 1122 6758
StyI C'CwwG_G
Cuts at: 107 837 875 2342 2843 5014 5368 107
Size: 730 38 1467 501 2171 354 2619
Fragments arranged by size:
2619 2171 1467 730 501 354 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1385 1859 5463 5596 6055 6264 1385
Size: 474 3604 133 459 209 3001
Fragments arranged by size:
3604 3001 474 459 209 133
TatI w'GTAC_w
Cuts at: 493 997 1343 2487 2540 2570 7395 493
Size: 504 346 1144 53 30 4825 978
Fragments arranged by size:
4825 1144 978 504 346 53 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3027 3059 7397 7429 3027
Size: 32 4338 32 3478
Fragments arranged by size:
4338 3478 32 32
Tth111I GACn'n_nGTC
Cuts at: 5677 5677
Size: 7880
XbaI T'CTAG_A
Cuts at: 4911 4911
Size: 7880
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5375 5375
Size: 7880
XmaI C'CCGG_G
Cuts at: 4900 4900
Size: 7880
XmnI GAAnn'nnTTC
Cuts at: 2377 2836 2953 3154 2377
Size: 459 117 201 7103
Fragments arranged by size:
7103 459 201 117
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PciI PflMI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
ApaI AscI AvrII BbvCI BcgI BglI BmtI Bpu10I
BseRI BsiWI BsrGI BssHII BstZ17I Bsu36I ClaI FseI
FspAI HpaI NheI NotI NruI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI StuI SwaI XhoI
ZraI