Restriction map for
pF1KA2011
(Circular) (Six-base) MAPSORT of: pF1KA2011.seq Check: 7444 from: 1 to: 6185
pF1KA2011 6185 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1107 1168 2805 1107
Size: 61 1637 4487
Fragments arranged by size:
4487 1637 61
AccI GT'mk_AC
Cuts at: 2378 3223 2378
Size: 845 5340
Acc65I G'GTAC_C
Cuts at: 1513 3201 1513
Size: 1688 4497
AclI AA'CG_TT
Cuts at: 6025 6025
Size: 6185
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1241 1803 1848 1966 2051 2860 2933 3016 4010
Size: 562 45 118 85 809 73 83 994
Cuts at: 4010 4442 4795 5457 1241
Size: 432 353 662 1969
Fragments arranged by size:
1969 994 809 662 562 432 353 118
85 83 73 45
AfeI AGC'GCT
Cuts at: 2787 2978 2787
Size: 191 5994
AflIII A'CryG_T
Cuts at: 291 3537 5337 5618 291
Size: 3246 1800 281 858
Fragments arranged by size:
3246 1800 858 281
AhdI GACnn_n'nnGTC
Cuts at: 1746 1746
Size: 6185
AlwNI CAG_nnn'CTG
Cuts at: 1087 1788 2419 2743 3490 4928 1087
Size: 701 631 324 747 1438 2344
Fragments arranged by size:
2344 1438 747 701 631 324
ApaI G_GGCC'C
Cuts at: 396 703 1078 1230 2394 396
Size: 307 375 152 1164 4187
Fragments arranged by size:
4187 1164 375 307 152
ApaLI G'TGCA_C
Cuts at: 2407 5023 2407
Size: 2616 3569
ApoI r'AATT_y
Cuts at: 829 3189 5538 5794 829
Size: 2360 2349 256 1220
Fragments arranged by size:
2360 2349 1220 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6185
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6185
AvaI C'yCGr_G
Cuts at: 272 388 782 2359 2438 2661 3119 3205 272
Size: 116 394 1577 79 223 458 86 3252
Fragments arranged by size:
3252 1577 458 394 223 116 86 79
AvrII C'CTAG_G
Cuts at: 2182 2182
Size: 6185
BamHI G'GATC_C
Cuts at: 2471 3210 2471
Size: 739 5446
BanI G'GyrC_C
Cuts at: 118 198 357 978 1357 1513 1841 2053 3201
Size: 80 159 621 379 156 328 212 1148
Cuts at: 3201 3615 3863 3898 118
Size: 414 248 35 2405
Fragments arranged by size:
2405 1148 621 414 379 328 248 212
159 156 80 35
BanII G_rGCy'C
Cuts at: 211 396 403 703 913 925 1078 1230 1288
Size: 185 7 300 210 12 153 152 58
Cuts at: 1288 1420 1604 2394 2546 3126 3199 4229 211
Size: 132 184 790 152 580 73 1030 2167
Fragments arranged by size:
2167 1030 790 580 300 210 185 184
153 152 152 132 73 58 12 7
BbeI G_GCGC'C
Cuts at: 2057 3619 3867 2057
Size: 1562 248 4375
Fragments arranged by size:
4375 1562 248
BbsI GAAGACnn'nnnn_
Cuts at: 1896 2437 2607 2637 1896
Size: 541 170 30 5444
Fragments arranged by size:
5444 541 170 30
BciVI GTATCCnnnnn_n'
Cuts at: 759 4083 5139 6185 759
Size: 3324 1056 1046 759
Fragments arranged by size:
3324 1056 1046 759
BclI T'GATC_A
Cuts at: 3705 3705
Size: 6185
BglI GCCn_nnn'nGGC
Cuts at: 1045 1082 1585 1834 1045
Size: 37 503 249 5396
Fragments arranged by size:
5396 503 249 37
BglII A'GATC_T
Cuts at: 3700 5737 3700
Size: 2037 4148
BlpI GC'TnA_GC
Cuts at: 869 1025 3125 3297 869
Size: 156 2100 172 3757
Fragments arranged by size:
3757 2100 172 156
Bme1580I G_kGCm'C
Cuts at: 203 360 396 703 983 1078 1230 1268 2394
Size: 157 36 307 280 95 152 38 1126
Cuts at: 2394 2411 3810 3903 5027 203
Size: 17 1399 93 1124 1361
Fragments arranged by size:
1399 1361 1126 1124 307 280 157 152
95 93 38 36 17
BmgBI CAC'GTC
Cuts at: 292 2613 292
Size: 2321 3864
BmrI ACTGGGnnnn_n'
Cuts at: 295 383 3397 3478 3813 5957 295
Size: 88 3014 81 335 2144 523
Fragments arranged by size:
3014 2144 523 335 88 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1059 2282 4552 1059
Size: 1223 2270 2692
Fragments arranged by size:
2692 2270 1223
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 449 1357 1405 1792 2173 2319 2689 2962 3066
Size: 908 48 387 381 146 370 273 104
Cuts at: 3066 3093 449
Size: 27 3541
Fragments arranged by size:
3541 908 387 381 370 273 146 104
48 27
Bpu10I CC'TnA_GC
Cuts at: 707 861 1672 707
Size: 154 811 5220
Fragments arranged by size:
5220 811 154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 495 897 1237 1876 2179 2461 2530 3361 4240
Size: 402 340 639 303 282 69 831 879
Cuts at: 4240 4954 5252 495
Size: 714 298 1428
Fragments arranged by size:
1428 879 831 714 639 402 340 303
298 282 69
BsaI GGTCTCn'nnnn_
Cuts at: 1963 5889 1963
Size: 3926 2259
BsaAI yAC'GTr
Cuts at: 3538 3538
Size: 6185
BsaBI GATnn'nnATC
Cuts at: 6 3699 5736 6
Size: 3693 2037 455
Fragments arranged by size:
3693 2037 455
BsaHI Gr'CG_yC
Cuts at: 2054 3616 3864 4566 6062 2054
Size: 1562 248 702 1496 2177
Fragments arranged by size:
2177 1562 1496 702 248
BsaWI w'CCGG_w
Cuts at: 545 1503 3374 3895 4573 4984 5131 5405 545
Size: 958 1871 521 678 411 147 274 1325
Fragments arranged by size:
1871 1325 958 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 497 608 666 783 1048 1570 1573 1576 2263
Size: 111 58 117 265 522 3 3 687
Cuts at: 2263 2266 2521 2824 3025 497
Size: 3 255 303 201 3657
Fragments arranged by size:
3657 687 522 303 265 255 201 117
111 58 3 3 3
BseYI C'CCAG_C
Cuts at: 213 485 522 693 856 1063 1135 1638 1879
Size: 272 37 171 163 207 72 503 241
Cuts at: 1879 2006 2736 2782 2879 2883 3610 4368 5033
Size: 127 730 46 97 4 727 758 665
Cuts at: 5033 5441 213
Size: 408 957
Fragments arranged by size:
957 758 730 727 665 503 408 272
241 207 171 163 127 97 72 46
37 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1439 1439
Size: 6185
BsiEI CG_ry'CG
Cuts at: 104 3727 3773 5003 104
Size: 3623 46 1230 1286
Fragments arranged by size:
3623 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 403 2411 2546 2870 3055 3199 3977 4167 5027
Size: 2008 135 324 185 144 778 190 860
Cuts at: 5027 403
Size: 1561
Fragments arranged by size:
2008 1561 860 778 324 190 185 144
135
BsmI GAATG_Cn'
Cuts at: 2556 2556
Size: 6185
BsmBI CGTCTCn'nnnn_
Cuts at: 2253 2530 2253
Size: 277 5908
Bsp1286I G_dGCh'C
Cuts at: 203 211 360 396 403 703 913 925 983
Size: 8 149 36 7 300 210 12 58
Cuts at: 983 1078 1230 1268 1288 1420 1604 2394 2411
Size: 95 152 38 20 132 184 790 17
Cuts at: 2411 2546 2870 3055 3126 3199 3810 3903 3977
Size: 135 324 185 71 73 611 93 74
Cuts at: 3977 4167 4229 5027 203
Size: 190 62 798 1361
Fragments arranged by size:
1361 798 790 611 324 300 210 190
185 184 152 149 135 132 95 93
74 73 71 62 58 38 36 20
17 12 8 7
BspHI T'CATG_A
Cuts at: 4617 4617
Size: 6185
BspMI ACCTGCnnnn'nnnn_
Cuts at: 250 468 744 1107 1168 2137 2504 2630 2805
Size: 218 276 363 61 969 367 126 175
Cuts at: 2805 3235 3449 3751 4132 250
Size: 430 214 302 381 2303
Fragments arranged by size:
2303 969 430 381 367 363 302 276
218 214 175 126 61
BsrBI CCG'CTC
Cuts at: 50 1029 1719 4477 4531 6015 6180 50
Size: 979 690 2758 54 1484 165 55
Fragments arranged by size:
2758 1484 979 690 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1158 4097 1158
Size: 2939 3246
BsrFI r'CCGG_y
Cuts at: 4183 4364 4183
Size: 181 6004
BsrGI T'GTAC_A
Cuts at: 307 627 2204 307
Size: 320 1577 4288
Fragments arranged by size:
4288 1577 320
BssSI C'ACGA_G
Cuts at: 2582 2827 4456 5164 2582
Size: 245 1629 708 3603
Fragments arranged by size:
3603 1629 708 245
BstAPI GCAn_nnn'nTGC
Cuts at: 2129 3562 2129
Size: 1433 4752
BstBI TT'CG_AA
Cuts at: 1325 4546 1325
Size: 3221 2964
BstXI CCAn_nnnn'nTGG
Cuts at: 179 693 179
Size: 514 5671
BstYI r'GATC_y
Cuts at: 1 489 2094 2471 3210 3677 3700 4035 4281
Size: 488 1605 377 739 467 23 335 246
Cuts at: 4281 4587 4599 4685 5737 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1605 1052 739 488 467 449 377 335
306 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 985 985
Size: 6185
BtgI C'CryG_G
Cuts at: 107 1105 2100 2466 2846 107
Size: 998 995 366 380 3446
Fragments arranged by size:
3446 998 995 380 366
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4135 4316 4479 4135
Size: 181 163 5841
Fragments arranged by size:
5841 181 163
BtsI GCAGTG_nn'
Cuts at: 1085 1152 2239 2328 2334 3012 3029 3168 3428
Size: 67 1087 89 6 678 17 139 260
Cuts at: 3428 1085
Size: 3842
Fragments arranged by size:
3842 1087 678 260 139 89 67 17
6
ClaI AT'CG_AT
Cuts at: 1010 1010
Size: 6185
DraI TTT'AAA
Cuts at: 3184 3184
Size: 6185
DraIII CAC_nnn'GTG
Cuts at: 337 337
Size: 6185
DrdI GACnn_nn'nnGTC
Cuts at: 2641 3891 5235 2641
Size: 1250 1344 3591
Fragments arranged by size:
3591 1344 1250
EaeI y'GGCC_r
Cuts at: 716 1046 1231 1790 2103 2279 2576 3148 3770
Size: 330 185 559 313 176 297 572 622
Cuts at: 3770 3944 4335 4362 5707 716
Size: 174 391 27 1345 1194
Fragments arranged by size:
1345 1194 622 572 559 391 330 313
297 185 176 174 27
EagI C'GGCC_G
Cuts at: 3770 3770
Size: 6185
EarI CTCTTCn'nnn_
Cuts at: 369 730 2452 2656 2909 4208 4418 369
Size: 361 1722 204 253 1299 210 2136
Fragments arranged by size:
2136 1722 1299 361 253 210 204
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3186 4393 5135 5281 5995 3186
Size: 1207 742 146 714 3376
Fragments arranged by size:
3376 1207 742 714 146
EcoICRI GAG'CTC
Cuts at: 401 2544 3197 401
Size: 2143 653 3389
Fragments arranged by size:
3389 2143 653
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 449 1241 1357 1405 1792 1803 1848 1966 2051
Size: 792 116 48 387 11 45 118 85
Cuts at: 2051 2173 2319 2689 2860 2933 2962 3016 3066
Size: 122 146 370 171 73 29 54 50
Cuts at: 3066 3093 4010 4442 4795 5457 449
Size: 27 917 432 353 662 1177
Fragments arranged by size:
1177 917 792 662 432 387 370 353
171 146 122 118 116 85 73 54
50 48 45 29 27 11
EcoNI CCTnn'n_nnAGG
Cuts at: 464 757 2500 2653 2803 3149 464
Size: 293 1743 153 150 346 3500
Fragments arranged by size:
3500 1743 346 293 153 150
EcoO109I rG'GnC_Cy
Cuts at: 268 392 699 700 733 885 1074 1226 2157
Size: 124 307 1 33 152 189 152 931
Cuts at: 2157 2390 2391 2526 2606 2658 3324 268
Size: 233 1 135 80 52 666 3129
Fragments arranged by size:
3129 931 666 307 233 189 152 152
135 124 80 52 33 1 1
EcoRI G'AATT_C
Cuts at: 829 3189 829
Size: 2360 3825
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4204 4204
Size: 6185
FspI TGC'GCA
Cuts at: 1781 2626 3966 1781
Size: 845 1340 4000
Fragments arranged by size:
4000 1340 845
HaeII r_GCGC'y
Cuts at: 714 1788 2057 2789 2980 3619 3867 5097 5868
Size: 1074 269 732 191 639 248 1230 771
Cuts at: 5868 714
Size: 1031
Fragments arranged by size:
1230 1074 1031 771 732 639 269 248
191
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 474 506 757 789 1681 1713 1732 1764 4027
Size: 32 251 32 892 32 19 32 2263
Cuts at: 4027 4057 4059 4089 474
Size: 30 2 30 2570
Fragments arranged by size:
2570 2263 892 251 32 32 32 32
30 30 19 2
HincII GTy'rAC
Cuts at: 3224 3224
Size: 6185
KasI G'GCGC_C
Cuts at: 2053 3615 3863 2053
Size: 1562 248 4375
Fragments arranged by size:
4375 1562 248
KpnI G_GTAC'C
Cuts at: 1517 3205 1517
Size: 1688 4497
MluI A'CGCG_T
Cuts at: 5618 5618
Size: 6185
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 460 566 636 1712 4944 5128 460
Size: 106 70 1076 3232 184 1517
Fragments arranged by size:
3232 1517 1076 184 106 70
MscI TGG'CCA
Cuts at: 718 1792 2578 3946 718
Size: 1074 786 1368 2957
Fragments arranged by size:
2957 1368 1074 786
MslI CAynn'nnrTG
Cuts at: 165 1912 4174 4301 165
Size: 1747 2262 127 2049
Fragments arranged by size:
2262 2049 1747 127
MspA1I CmG'CkG
Cuts at: 179 1680 2315 2632 2740 2883 2922 3296 3610
Size: 1501 635 317 108 143 39 374 314
Cuts at: 3610 3970 4754 4999 179
Size: 360 784 245 1365
Fragments arranged by size:
1501 1365 784 635 374 360 317 314
245 143 108 39
NaeI GCC'GGC
Cuts at: 4366 4366
Size: 6185
NarI GG'CG_CC
Cuts at: 2054 3616 3864 2054
Size: 1562 248 4375
Fragments arranged by size:
4375 1562 248
NcoI C'CATG_G
Cuts at: 107 2846 107
Size: 2739 3446
NgoMIV G'CCGG_C
Cuts at: 4364 4364
Size: 6185
NspI r_CATG'y
Cuts at: 225 3238 3738 5341 225
Size: 3013 500 1603 1069
Fragments arranged by size:
3013 1603 1069 500
PasI CC'CwG_GG
Cuts at: 114 353 1591 1728 114
Size: 239 1238 137 4571
Fragments arranged by size:
4571 1238 239 137
PciI A'CATG_T
Cuts at: 5337 5337
Size: 6185
PflMI CCAn_nnn'nTGG
Cuts at: 451 529 1123 2574 451
Size: 78 594 1451 4062
Fragments arranged by size:
4062 1451 594 78
PfoI T'CCnGG_A
Cuts at: 1334 2345 2683 1334
Size: 1011 338 4836
Fragments arranged by size:
4836 1011 338
PmeI GTTT'AAAC
Cuts at: 3184 3184
Size: 6185
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4631 4663 4631
Size: 32 6153
PpuMI rG'GwC_Cy
Cuts at: 268 733 268
Size: 465 5720
PspOMI G'GGCC_C
Cuts at: 392 699 1074 1226 2390 392
Size: 307 375 152 1164 4187
Fragments arranged by size:
4187 1164 375 307 152
PspXI vC'TCGA_Gb
Cuts at: 272 272
Size: 6185
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1879 1911 1879
Size: 32 6153
PstI C_TGCA'G
Cuts at: 2234 2428 2501 3098 3232 3917 2234
Size: 194 73 597 134 685 4502
Fragments arranged by size:
4502 685 597 194 134 73
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6185
PvuII CAG'CTG
Cuts at: 1680 2632 2740 2883 3610 3970 1680
Size: 952 108 143 727 360 3895
Fragments arranged by size:
3895 952 727 360 143 108
RsrII CG'GwC_CG
Cuts at: 4380 4380
Size: 6185
SacI G_AGCT'C
Cuts at: 403 2546 3199 403
Size: 2143 653 3389
Fragments arranged by size:
3389 2143 653
SalI G'TCGA_C
Cuts at: 3222 3222
Size: 6185
SapI GCTCTTCn'nnn_
Cuts at: 2452 2909 4208 4418 2452
Size: 457 1299 210 4219
Fragments arranged by size:
4219 1299 457 210
SbfI CC_TGCA'GG
Cuts at: 3232 3232
Size: 6185
ScaI AGT'ACT
Cuts at: 955 5702 955
Size: 4747 1438
SexAI A'CCwGG_T
Cuts at: 769 769
Size: 6185
SfcI C'TryA_G
Cuts at: 33 2230 2424 2497 2595 2961 3094 3228 3913
Size: 2197 194 73 98 366 133 134 685
Cuts at: 3913 4881 5072 33
Size: 968 191 1146
Fragments arranged by size:
2197 1146 968 685 366 194 191 134
133 98 73
SfiI GGCCn_nnn'nGGCC
Cuts at: 1045 1834 1045
Size: 789 5396
SfoI GGC'GCC
Cuts at: 2055 3617 3865 2055
Size: 1562 248 4375
Fragments arranged by size:
4375 1562 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6185
SmaI CCC'GGG
Cuts at: 390 784 3121 3207 390
Size: 394 2337 86 3368
Fragments arranged by size:
3368 2337 394 86
SmlI C'TyrA_G
Cuts at: 272 474 912 1252 1891 2194 2476 2545 3340
Size: 202 438 340 639 303 282 69 795
Cuts at: 3340 4255 4969 5231 272
Size: 915 714 262 1226
Fragments arranged by size:
1226 915 795 714 639 438 340 303
282 262 202 69
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6185
SphI G_CATG'C
Cuts at: 3238 3738 3238
Size: 500 5685
StuI AGG'CCT
Cuts at: 1216 1390 2200 2935 1216
Size: 174 810 735 4466
Fragments arranged by size:
4466 810 735 174
StyI C'CwwG_G
Cuts at: 107 528 845 1391 1537 1632 1653 2182 2294
Size: 421 317 546 146 95 21 529 112
Cuts at: 2294 2846 3319 3673 107
Size: 552 473 354 2619
Fragments arranged by size:
2619 552 546 529 473 421 354 317
146 112 95 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 384 484 1954 3768 3901 4360 4569 384
Size: 100 1470 1814 133 459 209 2000
Fragments arranged by size:
2000 1814 1470 459 209 133 100
TatI w'GTAC_w
Cuts at: 307 627 953 2204 2510 5700 307
Size: 320 326 1251 306 3190 792
Fragments arranged by size:
3190 1251 792 326 320 306
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5702 5734 5702
Size: 32 6153
Tth111I GACn'n_nGTC
Cuts at: 3982 3982
Size: 6185
XbaI T'CTAG_A
Cuts at: 3216 3216
Size: 6185
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1581 2343 3680 1581
Size: 762 1337 4086
Fragments arranged by size:
4086 1337 762
XhoI C'TCGA_G
Cuts at: 272 272
Size: 6185
XmaI C'CCGG_G
Cuts at: 388 782 3119 3205 388
Size: 394 2337 86 3368
Fragments arranged by size:
3368 2337 394 86
XmnI GAAnn'nnTTC
Cuts at: 2461 2461
Size: 6185
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AleI AlfI AloI AscI BaeI
BbvCI BcgI BfrBI BmtI BsaXI BsiWI BspEI BssHII
BstEII BstZ17I CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PmlI
PshAI PsiI SacII SanDI SgrAI SnaBI SrfI SspI
SwaI ZraI