Restriction map for
pF1KA2014
(Circular) (Six-base) MAPSORT of: pF1KA2014.seq Check: 5556 from: 1 to: 6194
pF1KA2014 6194 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1142 1200 1142
Size: 58 6136
AatII G_ACGT'C
Cuts at: 779 779
Size: 6194
AccI GT'mk_AC
Cuts at: 3232 3232
Size: 6194
Acc65I G'GTAC_C
Cuts at: 3079 3210 3079
Size: 131 6063
AclI AA'CG_TT
Cuts at: 6034 6034
Size: 6194
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 763 1857 2241 2416 4019 4451 4804 5466 763
Size: 1094 384 175 1603 432 353 662 1491
Fragments arranged by size:
1603 1491 1094 662 432 384 353 175
AflIII A'CryG_T
Cuts at: 1238 3546 5346 5627 1238
Size: 2308 1800 281 1805
Fragments arranged by size:
2308 1805 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1021 1973 2018 2366 1021
Size: 952 45 348 4849
Fragments arranged by size:
4849 952 348 45
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 202 234 202
Size: 32 6162
AlwNI CAG_nnn'CTG
Cuts at: 2173 2217 2394 2484 3499 4937 2173
Size: 44 177 90 1015 1438 3430
Fragments arranged by size:
3430 1438 1015 177 90 44
ApaI G_GGCC'C
Cuts at: 741 1957 741
Size: 1216 4978
ApaLI G'TGCA_C
Cuts at: 1099 1459 5032 1099
Size: 360 3573 2261
Fragments arranged by size:
3573 2261 360
ApoI r'AATT_y
Cuts at: 487 3198 5547 5803 487
Size: 2711 2349 256 878
Fragments arranged by size:
2711 2349 878 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6194
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6194
AvaI C'yCGr_G
Cuts at: 138 3170 3214 138
Size: 3032 44 3118
Fragments arranged by size:
3118 3032 44
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 771 804 771
Size: 33 6161
BamHI G'GATC_C
Cuts at: 3219 3219
Size: 6194
BanI G'GyrC_C
Cuts at: 3035 3079 3210 3624 3872 3907 3035
Size: 44 131 414 248 35 5322
Fragments arranged by size:
5322 414 248 131 44 35
BanII G_rGCy'C
Cuts at: 149 200 238 741 1957 3177 3208 4238 149
Size: 51 38 503 1216 1220 31 1030 2105
Fragments arranged by size:
2105 1220 1216 1030 503 51 38 31
BbeI G_GCGC'C
Cuts at: 3039 3628 3876 3039
Size: 589 248 5357
Fragments arranged by size:
5357 589 248
BbsI GAAGACnn'nnnn_
Cuts at: 1818 1863 2375 1818
Size: 45 512 5637
Fragments arranged by size:
5637 512 45
BbvCI CC'TCA_GC
Cuts at: 2081 2174 2234 2780 2081
Size: 93 60 546 5495
Fragments arranged by size:
5495 546 93 60
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 612 646 612
Size: 34 6160
BciVI GTATCCnnnnn_n'
Cuts at: 799 2493 4092 5148 6194 799
Size: 1694 1599 1056 1046 799
Fragments arranged by size:
1694 1599 1056 1046 799
BclI T'GATC_A
Cuts at: 3714 3714
Size: 6194
BglI GCCn_nnn'nGGC
Cuts at: 661 2776 661
Size: 2115 4079
BglII A'GATC_T
Cuts at: 449 1895 2087 3709 5746 449
Size: 1446 192 1622 2037 897
Fragments arranged by size:
2037 1622 1446 897 192
BlpI GC'TnA_GC
Cuts at: 232 3306 232
Size: 3074 3120
Bme1580I G_kGCm'C
Cuts at: 741 1103 1463 1957 3819 3912 5036 741
Size: 362 360 494 1862 93 1124 1899
Fragments arranged by size:
1899 1862 1124 494 362 360 93
BmgBI CAC'GTC
Cuts at: 783 2649 783
Size: 1866 4328
BmrI ACTGGGnnnn_n'
Cuts at: 377 540 2434 2848 3152 3406 3487 3822 5966
Size: 163 1894 414 304 254 81 335 2144
Cuts at: 5966 377
Size: 605
Fragments arranged by size:
2144 1894 605 414 335 304 254 163
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 168 4561 168
Size: 4393 1801
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 229 236 349 466 639 1312 1561 1613
Size: 90 7 113 117 173 673 249 52
Cuts at: 1613 2239 2461 2650 2743 3161 139
Size: 626 222 189 93 418 3172
Fragments arranged by size:
3172 673 626 418 249 222 189 173
117 113 93 90 52 7
Bpu10I CC'TnA_GC
Cuts at: 194 2081 2174 2234 2780 2951 194
Size: 1887 93 60 546 171 3437
Fragments arranged by size:
3437 1887 546 171 93 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3370 4249 4963 5261 3370
Size: 879 714 298 4303
Fragments arranged by size:
4303 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1267 5898 1267
Size: 4631 1563
BsaAI yAC'GTr
Cuts at: 3547 3547
Size: 6194
BsaBI GATnn'nnATC
Cuts at: 6 3708 5745 6
Size: 3702 2037 455
Fragments arranged by size:
3702 2037 455
BsaHI Gr'CG_yC
Cuts at: 776 2365 2768 3036 3625 3873 4575 6071 776
Size: 1589 403 268 589 248 702 1496 899
Fragments arranged by size:
1589 1496 899 702 589 403 268 248
BsaWI w'CCGG_w
Cuts at: 611 710 1037 2516 2717 3383 3904 4582 4993
Size: 99 327 1479 201 666 521 678 411
Cuts at: 4993 5140 5414 611
Size: 147 274 1391
Fragments arranged by size:
1479 1391 678 666 521 411 327 274
201 147 99
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 202 232 1306 1336 2820 2850 202
Size: 30 1074 30 1484 30 3546
Fragments arranged by size:
3546 1484 1074 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 423 962 1180 1318 1327 1507 1660 2098 2236
Size: 539 218 138 9 180 153 438 138
Cuts at: 2236 2797 2938 423
Size: 561 141 3679
Fragments arranged by size:
3679 561 539 438 218 180 153 141
138 138 9
BseYI C'CCAG_C
Cuts at: 649 1155 1722 2279 2658 2987 3619 4377 5042
Size: 506 567 557 379 329 632 758 665
Cuts at: 5042 5450 649
Size: 408 1393
Fragments arranged by size:
1393 758 665 632 567 557 506 408
379 329
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 991 1234 1730 2581 2712 3134 436
Size: 555 243 496 851 131 422 3496
Fragments arranged by size:
3496 851 555 496 422 243 131
BsiEI CG_ry'CG
Cuts at: 104 3736 3782 5012 104
Size: 3632 46 1230 1286
Fragments arranged by size:
3632 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 238 710 1103 1463 3208 3986 4176 5036 238
Size: 472 393 360 1745 778 190 860 1396
Fragments arranged by size:
1745 1396 860 778 472 393 360 190
Bsp1286I G_dGCh'C
Cuts at: 149 200 238 710 741 1103 1463 1957 3177
Size: 51 38 472 31 362 360 494 1220
Cuts at: 3177 3208 3819 3912 3986 4176 4238 5036 149
Size: 31 611 93 74 190 62 798 1307
Fragments arranged by size:
1307 1220 798 611 494 472 362 360
190 93 74 62 51 38 31 31
BspEI T'CCGG_A
Cuts at: 1037 2717 1037
Size: 1680 4514
BspHI T'CATG_A
Cuts at: 809 4626 809
Size: 3817 2377
BspMI ACCTGCnnnn'nnnn_
Cuts at: 254 614 647 1142 1200 2333 3244 3458 3760
Size: 360 33 495 58 1133 911 214 302
Cuts at: 3760 4141 254
Size: 381 2307
Fragments arranged by size:
2307 1133 911 495 381 360 302 214
58 33
BsrBI CCG'CTC
Cuts at: 50 2075 2202 2208 4486 4540 6024 6189 50
Size: 2025 127 6 2278 54 1484 165 55
Fragments arranged by size:
2278 2025 1484 165 127 55 54 6
BsrDI GCAATG_nn'
Cuts at: 2357 4106 2357
Size: 1749 4445
BsrFI r'CCGG_y
Cuts at: 3053 4192 4373 3053
Size: 1139 181 4874
Fragments arranged by size:
4874 1139 181
BssHII G'CGCG_C
Cuts at: 694 694
Size: 6194
BssSI C'ACGA_G
Cuts at: 4465 5173 4465
Size: 708 5486
BstAPI GCAn_nnn'nTGC
Cuts at: 1467 2595 3571 1467
Size: 1128 976 4090
Fragments arranged by size:
4090 1128 976
BstBI TT'CG_AA
Cuts at: 1785 4555 1785
Size: 2770 3424
BstXI CCAn_nnnn'nTGG
Cuts at: 1861 2046 2967 1861
Size: 185 921 5088
Fragments arranged by size:
5088 921 185
BstYI r'GATC_y
Cuts at: 1 300 449 1895 1911 2087 2487 3219 3686
Size: 299 149 1446 16 176 400 732 467
Cuts at: 3686 3709 4044 4290 4596 4608 4694 5746 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1446 1052 732 467 449 400 335 306
299 246 176 149 86 23 16 12
Bsu36I CC'TnA_GG
Cuts at: 1643 1643
Size: 6194
BtgI C'CryG_G
Cuts at: 107 107
Size: 6194
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2526 4144 4325 4488 2526
Size: 1618 181 163 4232
Fragments arranged by size:
4232 1618 181 163
BtsI GCAGTG_nn'
Cuts at: 2527 2627 3108 3437 2527
Size: 100 481 329 5284
Fragments arranged by size:
5284 481 329 100
DraI TTT'AAA
Cuts at: 3193 3193
Size: 6194
DraIII CAC_nnn'GTG
Cuts at: 1107 1458 1107
Size: 351 5843
DrdI GACnn_nn'nnGTC
Cuts at: 781 2710 3900 5244 781
Size: 1929 1190 1344 1731
Fragments arranged by size:
1929 1731 1344 1190
EaeI y'GGCC_r
Cuts at: 1565 2048 2070 3779 3953 4344 4371 5716 1565
Size: 483 22 1709 174 391 27 1345 2043
Fragments arranged by size:
2043 1709 1345 483 391 174 27 22
EagI C'GGCC_G
Cuts at: 3779 3779
Size: 6194
EarI CTCTTCn'nnn_
Cuts at: 461 1664 2260 4217 4427 461
Size: 1203 596 1957 210 2228
Fragments arranged by size:
2228 1957 1203 596 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1667 4402 5144 5290 6004 1667
Size: 2735 742 146 714 1857
Fragments arranged by size:
2735 1857 742 714 146
EcoICRI GAG'CTC
Cuts at: 236 3206 236
Size: 2970 3224
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 229 236 349 466 639 763 1312 1561
Size: 90 7 113 117 173 124 549 249
Cuts at: 1561 1613 1857 2239 2241 2416 2461 2650 2743
Size: 52 244 382 2 175 45 189 93
Cuts at: 2743 3161 4019 4451 4804 5466 139
Size: 418 858 432 353 662 867
Fragments arranged by size:
867 858 662 549 432 418 382 353
249 244 189 175 173 124 117 113
93 90 52 45 7 2
EcoNI CCTnn'n_nnAGG
Cuts at: 621 1294 2062 621
Size: 673 768 4753
Fragments arranged by size:
4753 768 673
EcoO109I rG'GnC_Cy
Cuts at: 135 636 737 738 1536 1560 1576 1650 1905
Size: 501 101 1 798 24 16 74 255
Cuts at: 1905 1953 1954 3181 3333 135
Size: 48 1 1227 152 2996
Fragments arranged by size:
2996 1227 798 501 255 152 101 74
48 24 16 1 1
EcoRI G'AATT_C
Cuts at: 3198 3198
Size: 6194
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4213 4213
Size: 6194
FspI TGC'GCA
Cuts at: 3975 3975
Size: 6194
HaeII r_GCGC'y
Cuts at: 128 656 3039 3628 3876 5106 5877 128
Size: 528 2383 589 248 1230 771 445
Fragments arranged by size:
2383 1230 771 589 528 445 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 520 552 1306 1338 2472 2491 2504 2523 2820
Size: 32 754 32 1134 19 13 19 297
Cuts at: 2820 2852 4036 4066 4068 4098 520
Size: 32 1184 30 2 30 2616
Fragments arranged by size:
2616 1184 1134 754 297 32 32 32
30 30 19 19 13 2
HincII GTy'rAC
Cuts at: 425 1763 2276 3233 425
Size: 1338 513 957 3386
Fragments arranged by size:
3386 1338 957 513
HindIII A'AGCT_T
Cuts at: 1339 1339
Size: 6194
KasI G'GCGC_C
Cuts at: 3035 3624 3872 3035
Size: 589 248 5357
Fragments arranged by size:
5357 589 248
KpnI G_GTAC'C
Cuts at: 3083 3214 3083
Size: 131 6063
MluI A'CGCG_T
Cuts at: 5627 5627
Size: 6194
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1241 1289 1634 2003 2387 4953 5137 1241
Size: 48 345 369 384 2566 184 2298
Fragments arranged by size:
2566 2298 384 369 345 184 48
MscI TGG'CCA
Cuts at: 1567 2050 3955 1567
Size: 483 1905 3806
Fragments arranged by size:
3806 1905 483
MslI CAynn'nnrTG
Cuts at: 851 1096 4183 4310 851
Size: 245 3087 127 2735
Fragments arranged by size:
3087 2735 245 127
MspA1I CmG'CkG
Cuts at: 931 1395 1404 2233 2763 2946 2991 3305 3619
Size: 464 9 829 530 183 45 314 314
Cuts at: 3619 3979 4763 5008 931
Size: 360 784 245 2117
Fragments arranged by size:
2117 829 784 530 464 360 314 314
245 183 45 9
NaeI GCC'GGC
Cuts at: 4375 4375
Size: 6194
NarI GG'CG_CC
Cuts at: 3036 3625 3873 3036
Size: 589 248 5357
Fragments arranged by size:
5357 589 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 6194
NdeI CA'TA_TG
Cuts at: 1437 1437
Size: 6194
NgoMIV G'CCGG_C
Cuts at: 4373 4373
Size: 6194
NspI r_CATG'y
Cuts at: 1080 1321 1602 3247 3747 5350 1080
Size: 241 281 1645 500 1603 1924
Fragments arranged by size:
1924 1645 1603 500 281 241
PasI CC'CwG_GG
Cuts at: 727 727
Size: 6194
PciI A'CATG_T
Cuts at: 5346 5346
Size: 6194
PflMI CCAn_nnn'nTGG
Cuts at: 2286 2484 3120 2286
Size: 198 636 5360
Fragments arranged by size:
5360 636 198
PfoI T'CCnGG_A
Cuts at: 616 2315 616
Size: 1699 4495
PmeI GTTT'AAAC
Cuts at: 3193 3193
Size: 6194
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 202 234 4640 4672 202
Size: 32 4406 32 1724
Fragments arranged by size:
4406 1724 32 32
PpuMI rG'GwC_Cy
Cuts at: 135 636 1650 1905 135
Size: 501 1014 255 4424
Fragments arranged by size:
4424 1014 501 255
PshAI GACnn'nnGTC
Cuts at: 1715 1715
Size: 6194
PspOMI G'GGCC_C
Cuts at: 737 1953 737
Size: 1216 4978
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 805 837 805
Size: 32 6162
PstI C_TGCA'G
Cuts at: 644 1139 1178 1211 1584 2095 2330 2619 3241
Size: 495 39 33 373 511 235 289 622
Cuts at: 3241 3926 644
Size: 685 2912
Fragments arranged by size:
2912 685 622 511 495 373 289 235
39 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6194
PvuII CAG'CTG
Cuts at: 931 1395 2233 2946 3619 3979 931
Size: 464 838 713 673 360 3146
Fragments arranged by size:
3146 838 713 673 464 360
RsrII CG'GwC_CG
Cuts at: 4389 4389
Size: 6194
SacI G_AGCT'C
Cuts at: 238 3208 238
Size: 2970 3224
SalI G'TCGA_C
Cuts at: 3231 3231
Size: 6194
SanDI GG'GwC_CC
Cuts at: 135 135
Size: 6194
SapI GCTCTTCn'nnn_
Cuts at: 2260 4217 4427 2260
Size: 1957 210 4027
Fragments arranged by size:
4027 1957 210
SbfI CC_TGCA'GG
Cuts at: 3241 3241
Size: 6194
ScaI AGT'ACT
Cuts at: 434 5711 434
Size: 5277 917
SexAI A'CCwGG_T
Cuts at: 833 1454 833
Size: 621 5573
SfcI C'TryA_G
Cuts at: 33 640 1135 1174 1207 1399 1580 2091 2326
Size: 607 495 39 33 192 181 511 235
Cuts at: 2326 2615 3237 3922 4890 5081 33
Size: 289 622 685 968 191 1146
Fragments arranged by size:
1146 968 685 622 607 511 495 289
235 192 191 181 39 33
SfoI GGC'GCC
Cuts at: 3037 3626 3874 3037
Size: 589 248 5357
Fragments arranged by size:
5357 589 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6194
SmaI CCC'GGG
Cuts at: 140 3216 140
Size: 3076 3118
SmlI C'TyrA_G
Cuts at: 3349 4264 4978 5240 3349
Size: 915 714 262 4303
Fragments arranged by size:
4303 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6194
SphI G_CATG'C
Cuts at: 1080 1602 3247 3747 1080
Size: 522 1645 500 3527
Fragments arranged by size:
3527 1645 522 500
SspI AAT'ATT
Cuts at: 1056 1056
Size: 6194
StuI AGG'CCT
Cuts at: 512 1498 2788 3104 512
Size: 986 1290 316 3602
Fragments arranged by size:
3602 1290 986 316
StyI C'CwwG_G
Cuts at: 107 895 3044 3328 3682 107
Size: 788 2149 284 354 2619
Fragments arranged by size:
2619 2149 788 354 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 464 468 663 3777 3910 4369 4578 464
Size: 4 195 3114 133 459 209 2080
Fragments arranged by size:
3114 2080 459 209 195 133 4
TatI w'GTAC_w
Cuts at: 432 519 5709 432
Size: 87 5190 917
Fragments arranged by size:
5190 917 87
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5711 5743 5711
Size: 32 6162
Tth111I GACn'n_nGTC
Cuts at: 3991 3991
Size: 6194
XbaI T'CTAG_A
Cuts at: 3225 3225
Size: 6194
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2287 3689 114
Size: 2173 1402 2619
Fragments arranged by size:
2619 2173 1402
XmaI C'CCGG_G
Cuts at: 138 3214 138
Size: 3076 3118
XmnI GAAnn'nnTTC
Cuts at: 400 400
Size: 6194
ZraI GAC'GTC
Cuts at: 777 777
Size: 6194
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AleI AscI AvrII BfrBI
BmtI BsiWI BsmI BsmBI BsrGI BstEII BstZ17I ClaI
CspCI EcoRV FseI FspAI HpaI MfeI NheI NotI
NruI NsiI PacI PmlI PsiI PspXI SacII SfiI
SgrAI SnaBI SrfI SwaI XhoI