Restriction map for
pF1KA2027
(Circular) (Six-base) MAPSORT of: pF1KA2027.seq Check: 3164 from: 1 to: 5441
pF1KA2027 5441 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 387 517 387
Size: 130 5311
AatII G_ACGT'C
Cuts at: 2159 2159
Size: 5441
AccI GT'mk_AC
Cuts at: 1309 1759 2479 1309
Size: 450 720 4271
Fragments arranged by size:
4271 720 450
Acc65I G'GTAC_C
Cuts at: 2457 2457
Size: 5441
AclI AA'CG_TT
Cuts at: 5281 5281
Size: 5441
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 581 785 1159 3266 3698 4051 4713 581
Size: 204 374 2107 432 353 662 1309
Fragments arranged by size:
2107 1309 662 432 374 353 204
AflIII A'CryG_T
Cuts at: 1405 2793 4593 4874 1405
Size: 1388 1800 281 1972
Fragments arranged by size:
1972 1800 1388 281
AhdI GACnn_n'nnGTC
Cuts at: 2214 2214
Size: 5441
AleI CACnn'nnGTG
Cuts at: 1199 1199
Size: 5441
AlwNI CAG_nnn'CTG
Cuts at: 1963 2062 2065 2746 4184 1963
Size: 99 3 681 1438 3220
Fragments arranged by size:
3220 1438 681 99 3
ApaI G_GGCC'C
Cuts at: 877 877
Size: 5441
ApaLI G'TGCA_C
Cuts at: 1282 4279 1282
Size: 2997 2444
ApoI r'AATT_y
Cuts at: 1567 2445 4794 5050 1567
Size: 878 2349 256 1958
Fragments arranged by size:
2349 1958 878 256
AseI AT'TA_AT
Cuts at: 20 2275 20
Size: 2255 3186
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5441
AvaI C'yCGr_G
Cuts at: 444 870 2461 444
Size: 426 1591 3424
Fragments arranged by size:
3424 1591 426
BamHI G'GATC_C
Cuts at: 1689 2466 1689
Size: 777 4664
BanI G'GyrC_C
Cuts at: 253 957 2457 2871 3119 3154 253
Size: 704 1500 414 248 35 2540
Fragments arranged by size:
2540 1500 704 414 248 35
BanII G_rGCy'C
Cuts at: 877 1223 2455 3485 877
Size: 346 1232 1030 2833
Fragments arranged by size:
2833 1232 1030 346
BbeI G_GCGC'C
Cuts at: 2875 3123 2875
Size: 248 5193
BbsI GAAGACnn'nnnn_
Cuts at: 179 335 1978 2414 179
Size: 156 1643 436 3206
Fragments arranged by size:
3206 1643 436 156
BbvCI CC'TCA_GC
Cuts at: 274 274
Size: 5441
BciVI GTATCCnnnnn_n'
Cuts at: 1132 3339 4395 5441 1132
Size: 2207 1056 1046 1132
Fragments arranged by size:
2207 1132 1056 1046
BclI T'GATC_A
Cuts at: 528 1671 2961 528
Size: 1143 1290 3008
Fragments arranged by size:
3008 1290 1143
BglI GCCn_nnn'nGGC
Cuts at: 881 881
Size: 5441
BglII A'GATC_T
Cuts at: 2956 4993 2956
Size: 2037 3404
BlpI GC'TnA_GC
Cuts at: 2553 2553
Size: 5441
Bme1580I G_kGCm'C
Cuts at: 258 877 1264 1286 3066 3159 4283 258
Size: 619 387 22 1780 93 1124 1416
Fragments arranged by size:
1780 1416 1124 619 387 93 22
BmrI ACTGGGnnnn_n'
Cuts at: 1112 2653 2734 3069 5213 1112
Size: 1541 81 335 2144 1340
Fragments arranged by size:
2144 1541 1340 335 81
BmtI G_CTAG'C
Cuts at: 1240 1240
Size: 5441
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 655 3808 655
Size: 3153 2288
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 665 695 1237 1371 1424 1935 2069 2110 665
Size: 30 542 134 53 511 134 41 3996
Fragments arranged by size:
3996 542 511 134 134 53 41 30
Bpu10I CC'TnA_GC
Cuts at: 274 1157 1921 2069 274
Size: 883 764 148 3646
Fragments arranged by size:
3646 883 764 148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2017 2617 3496 4210 4508 2017
Size: 600 879 714 298 2950
Fragments arranged by size:
2950 879 714 600 298
BsaI GGTCTCn'nnnn_
Cuts at: 316 1389 5145 316
Size: 1073 3756 612
Fragments arranged by size:
3756 1073 612
BsaAI yAC'GTr
Cuts at: 1406 2794 1406
Size: 1388 4053
BsaBI GATnn'nnATC
Cuts at: 6 1522 2955 4992 6
Size: 1516 1433 2037 455
Fragments arranged by size:
2037 1516 1433 455
BsaHI Gr'CG_yC
Cuts at: 635 2156 2391 2872 3120 3822 5318 635
Size: 1521 235 481 248 702 1496 758
Fragments arranged by size:
1521 1496 758 702 481 248 235
BsaWI w'CCGG_w
Cuts at: 163 2630 3151 3829 4240 4387 4661 163
Size: 2467 521 678 411 147 274 943
Fragments arranged by size:
2467 943 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 206 236 2029 2059 206
Size: 30 1793 30 3588
Fragments arranged by size:
3588 1793 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1724 1724
Size: 5441
BseYI C'CCAG_C
Cuts at: 407 1045 1397 2011 2866 3624 4289 4697 407
Size: 638 352 614 855 758 665 408 1151
Fragments arranged by size:
1151 855 758 665 638 614 408 352
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 724 1093 388
Size: 336 369 4736
Fragments arranged by size:
4736 369 336
BsiEI CG_ry'CG
Cuts at: 104 2983 3029 4259 104
Size: 2879 46 1230 1286
Fragments arranged by size:
2879 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1155 1223 1286 1882 2455 3233 3423 4283 1155
Size: 68 63 596 573 778 190 860 2313
Fragments arranged by size:
2313 860 778 596 573 190 68 63
BsmI GAATG_Cn'
Cuts at: 240 240
Size: 5441
BsmBI CGTCTCn'nnnn_
Cuts at: 1110 1110
Size: 5441
Bsp1286I G_dGCh'C
Cuts at: 258 877 1155 1223 1264 1286 1882 2455 3066
Size: 619 278 68 41 22 596 573 611
Cuts at: 3066 3159 3233 3423 3485 4283 258
Size: 93 74 190 62 798 1416
Fragments arranged by size:
1416 798 619 611 596 573 278 190
93 74 68 62 41 22
BspEI T'CCGG_A
Cuts at: 163 163
Size: 5441
BspHI T'CATG_A
Cuts at: 3873 3873
Size: 5441
BspMI ACCTGCnnnn'nnnn_
Cuts at: 297 387 517 1465 2491 2705 3007 3388 297
Size: 90 130 948 1026 214 302 381 2350
Fragments arranged by size:
2350 1026 948 381 302 214 130 90
BsrBI CCG'CTC
Cuts at: 50 3733 3787 5271 5436 50
Size: 3683 54 1484 165 55
Fragments arranged by size:
3683 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3353 3353
Size: 5441
BsrFI r'CCGG_y
Cuts at: 3439 3620 3439
Size: 181 5260
BssSI C'ACGA_G
Cuts at: 3712 4420 3712
Size: 708 4733
BstAPI GCAn_nnn'nTGC
Cuts at: 2065 2818 2065
Size: 753 4688
BstBI TT'CG_AA
Cuts at: 3802 3802
Size: 5441
BstXI CCAn_nnnn'nTGG
Cuts at: 1201 1264 1819 1201
Size: 63 555 4823
Fragments arranged by size:
4823 555 63
BstYI r'GATC_y
Cuts at: 1 465 1099 1689 2305 2466 2933 2956 3291
Size: 464 634 590 616 161 467 23 335
Cuts at: 3291 3537 3843 3855 3941 4993 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 634 616 590 467 464 449 335
306 246 161 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1736 1736
Size: 5441
BtgI C'CryG_G
Cuts at: 107 1940 107
Size: 1833 3608
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3391 3572 3735 3391
Size: 181 163 5097
Fragments arranged by size:
5097 181 163
BtsI GCAGTG_nn'
Cuts at: 863 1085 1127 1206 2684 863
Size: 222 42 79 1478 3620
Fragments arranged by size:
3620 1478 222 79 42
DraI TTT'AAA
Cuts at: 2440 2440
Size: 5441
DrdI GACnn_nn'nnGTC
Cuts at: 3147 4491 3147
Size: 1344 4097
EaeI y'GGCC_r
Cuts at: 1611 3026 3200 3591 3618 4963 1611
Size: 1415 174 391 27 1345 2089
Fragments arranged by size:
2089 1415 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3026 3026
Size: 5441
EarI CTCTTCn'nnn_
Cuts at: 1445 3464 3674 1445
Size: 2019 210 3212
Fragments arranged by size:
3212 2019 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3649 4391 4537 5251 3649
Size: 742 146 714 3839
Fragments arranged by size:
3839 742 714 146
EcoICRI GAG'CTC
Cuts at: 1221 2453 1221
Size: 1232 4209
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 581 665 695 785 1159 1237 1371 1424 1935
Size: 84 30 90 374 78 134 53 511
Cuts at: 1935 2069 2110 3266 3698 4051 4713 581
Size: 134 41 1156 432 353 662 1309
Fragments arranged by size:
1309 1156 662 511 432 374 353 134
134 90 84 78 53 41 30
EcoNI CCTnn'n_nnAGG
Cuts at: 304 629 304
Size: 325 5116
EcoO109I rG'GnC_Cy
Cuts at: 806 874 1356 2580 806
Size: 68 482 1224 3667
Fragments arranged by size:
3667 1224 482 68
EcoRI G'AATT_C
Cuts at: 2445 2445
Size: 5441
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3460 3460
Size: 5441
FspI TGC'GCA
Cuts at: 3222 3222
Size: 5441
HaeII r_GCGC'y
Cuts at: 2875 3123 4353 5124 2875
Size: 248 1230 771 3192
Fragments arranged by size:
3192 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 206 238 646 678 1084 1116 1674 1706 1965
Size: 32 408 32 406 32 558 32 259
Cuts at: 1965 1997 2200 2232 3283 3313 3315 3345 206
Size: 32 203 32 1051 30 2 30 2302
Fragments arranged by size:
2302 1051 558 408 406 259 203 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1457 2480 1457
Size: 1023 4418
KasI G'GCGC_C
Cuts at: 2871 3119 2871
Size: 248 5193
KpnI G_GTAC'C
Cuts at: 2461 2461
Size: 5441
MluI A'CGCG_T
Cuts at: 4874 4874
Size: 5441
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1344 2412 4200 4384 1344
Size: 1068 1788 184 2401
Fragments arranged by size:
2401 1788 1068 184
MscI TGG'CCA
Cuts at: 3202 3202
Size: 5441
MslI CAynn'nnrTG
Cuts at: 1199 3430 3557 1199
Size: 2231 127 3083
Fragments arranged by size:
3083 2231 127
MspA1I CmG'CkG
Cuts at: 289 411 963 1179 1297 1303 1837 2062 2130
Size: 122 552 216 118 6 534 225 68
Cuts at: 2130 2552 2866 3226 4010 4255 289
Size: 422 314 360 784 245 1475
Fragments arranged by size:
1475 784 552 534 422 360 314 245
225 216 122 118 68 6
NaeI GCC'GGC
Cuts at: 3622 3622
Size: 5441
NarI GG'CG_CC
Cuts at: 2872 3120 2872
Size: 248 5193
NcoI C'CATG_G
Cuts at: 107 1940 107
Size: 1833 3608
NgoMIV G'CCGG_C
Cuts at: 3620 3620
Size: 5441
NheI G'CTAG_C
Cuts at: 1236 1236
Size: 5441
NruI TCG'CGA
Cuts at: 2230 2230
Size: 5441
NspI r_CATG'y
Cuts at: 938 1535 2494 2994 4597 938
Size: 597 959 500 1603 1782
Fragments arranged by size:
1782 1603 959 597 500
PasI CC'CwG_GG
Cuts at: 281 2220 281
Size: 1939 3502
PciI A'CATG_T
Cuts at: 4593 4593
Size: 5441
PflMI CCAn_nnn'nTGG
Cuts at: 1215 1963 1215
Size: 748 4693
PfoI T'CCnGG_A
Cuts at: 2085 2085
Size: 5441
PmeI GTTT'AAAC
Cuts at: 2440 2440
Size: 5441
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 257 289 3887 3919 257
Size: 32 3598 32 1779
Fragments arranged by size:
3598 1779 32 32
PpuMI rG'GwC_Cy
Cuts at: 806 1356 806
Size: 550 4891
PspOMI G'GGCC_C
Cuts at: 873 873
Size: 5441
PstI C_TGCA'G
Cuts at: 264 456 1302 2381 2488 3173 264
Size: 192 846 1079 107 685 2532
Fragments arranged by size:
2532 1079 846 685 192 107
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5441
PvuII CAG'CTG
Cuts at: 289 411 1179 1297 1303 1837 2062 2130 2866
Size: 122 768 118 6 534 225 68 736
Cuts at: 2866 3226 289
Size: 360 2504
Fragments arranged by size:
2504 768 736 534 360 225 122 118
68 6
RsrII CG'GwC_CG
Cuts at: 3636 3636
Size: 5441
SacI G_AGCT'C
Cuts at: 1223 2455 1223
Size: 1232 4209
SalI G'TCGA_C
Cuts at: 2478 2478
Size: 5441
SapI GCTCTTCn'nnn_
Cuts at: 3464 3674 3464
Size: 210 5231
SbfI CC_TGCA'GG
Cuts at: 2488 2488
Size: 5441
ScaI AGT'ACT
Cuts at: 4958 4958
Size: 5441
SexAI A'CCwGG_T
Cuts at: 1347 1347
Size: 5441
SfcI C'TryA_G
Cuts at: 33 260 452 752 1298 1310 2377 2484 3169
Size: 227 192 300 546 12 1067 107 685
Cuts at: 3169 4137 4328 33
Size: 968 191 1146
Fragments arranged by size:
1146 1067 968 685 546 300 227 192
191 107 12
SfoI GGC'GCC
Cuts at: 2873 3121 2873
Size: 248 5193
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5441
SmaI CCC'GGG
Cuts at: 2463 2463
Size: 5441
SmlI C'TyrA_G
Cuts at: 2032 2596 3511 4225 4487 2032
Size: 564 915 714 262 2986
Fragments arranged by size:
2986 915 714 564 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5441
SphI G_CATG'C
Cuts at: 1535 2494 2994 1535
Size: 959 500 3982
Fragments arranged by size:
3982 959 500
StyI C'CwwG_G
Cuts at: 107 879 1940 2204 2575 2929 107
Size: 772 1061 264 371 354 2619
Fragments arranged by size:
2619 1061 772 371 354 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 343 1933 3024 3157 3616 3825 343
Size: 1590 1091 133 459 209 1959
Fragments arranged by size:
1959 1590 1091 459 209 133
TatI w'GTAC_w
Cuts at: 4956 4956
Size: 5441
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2146 2178 4958 4990 2146
Size: 32 2780 32 2597
Fragments arranged by size:
2780 2597 32 32
Tth111I GACn'n_nGTC
Cuts at: 3238 3238
Size: 5441
XbaI T'CTAG_A
Cuts at: 2472 2472
Size: 5441
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2936 2936
Size: 5441
XmaI C'CCGG_G
Cuts at: 2461 2461
Size: 5441
ZraI GAC'GTC
Cuts at: 2157 2157
Size: 5441
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NruI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AlfI AloI AscI AvrII BaeI
BcgI BfrBI BmgBI BsiWI BsrGI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NotI NsiI PacI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI