Restriction map for
pF1KA2032
(Circular) (Six-base) MAPSORT of: pF1KA2032.seq Check: 1618 from: 1 to: 5945
pF1KA2032 5945 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 948 948
Size: 5945
AccI GT'mk_AC
Cuts at: 2983 2983
Size: 5945
Acc65I G'GTAC_C
Cuts at: 774 1870 2961 774
Size: 1096 1091 3758
Fragments arranged by size:
3758 1096 1091
AclI AA'CG_TT
Cuts at: 5785 5785
Size: 5945
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1606 1628 2351 2867 3770 4202 4949 5217 1606
Size: 22 723 516 903 432 747 268 2334
Fragments arranged by size:
2334 903 747 723 516 432 268 22
AflIII A'CryG_T
Cuts at: 3297 4401 5378 3297
Size: 1104 977 3864
Fragments arranged by size:
3864 1104 977
AgeI A'CCGG_T
Cuts at: 4333 4333
Size: 5945
AleI CACnn'nnGTG
Cuts at: 1816 1816
Size: 5945
AlwNI CAG_nnn'CTG
Cuts at: 1006 3250 4817 1006
Size: 2244 1567 2134
Fragments arranged by size:
2244 2134 1567
ApaLI G'TGCA_C
Cuts at: 4715 4715
Size: 5945
ApoI r'AATT_y
Cuts at: 402 578 650 1368 2949 5298 5554 402
Size: 176 72 718 1581 2349 256 793
Fragments arranged by size:
2349 1581 793 718 256 176 72
AseI AT'TA_AT
Cuts at: 20 983 2281 20
Size: 963 1298 3684
Fragments arranged by size:
3684 1298 963
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5945
AvaI C'yCGr_G
Cuts at: 2526 2727 2965 2526
Size: 201 238 5506
Fragments arranged by size:
5506 238 201
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1854 1887 1854
Size: 33 5912
BamHI G'GATC_C
Cuts at: 2970 2970
Size: 5945
BanI G'GyrC_C
Cuts at: 774 1870 2538 2961 3375 3623 3658 774
Size: 1096 668 423 414 248 35 3061
Fragments arranged by size:
3061 1096 668 423 414 248 35
BanII G_rGCy'C
Cuts at: 257 1403 1940 2959 3989 257
Size: 1146 537 1019 1030 2213
Fragments arranged by size:
2213 1146 1030 1019 537
BbeI G_GCGC'C
Cuts at: 2542 3379 3627 2542
Size: 837 248 4860
Fragments arranged by size:
4860 837 248
BbsI GAAGACnn'nnnn_
Cuts at: 564 851 564
Size: 287 5658
BbvCI CC'TCA_GC
Cuts at: 1071 1088 1839 2622 1071
Size: 17 751 783 4394
Fragments arranged by size:
4394 783 751 17
BciVI GTATCCnnnnn_n'
Cuts at: 193 2743 3843 4604 5945 193
Size: 2550 1100 761 1341 193
Fragments arranged by size:
2550 1341 1100 761 193
BclI T'GATC_A
Cuts at: 1925 3465 1925
Size: 1540 4405
BglII A'GATC_T
Cuts at: 1878 3460 5497 1878
Size: 1582 2037 2326
Fragments arranged by size:
2326 2037 1582
BlpI GC'TnA_GC
Cuts at: 2855 3057 2855
Size: 202 5743
Bme1580I G_kGCm'C
Cuts at: 2054 3570 3663 4719 2054
Size: 1516 93 1056 3280
Fragments arranged by size:
3280 1516 1056 93
BmgBI CAC'GTC
Cuts at: 1814 1814
Size: 5945
BmrI ACTGGGnnnn_n'
Cuts at: 252 964 3157 3238 3573 5717 252
Size: 712 2193 81 335 2144 480
Fragments arranged by size:
2193 2144 712 480 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4312 4312
Size: 5945
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1688 2082 2585 1688
Size: 394 503 5048
Fragments arranged by size:
5048 503 394
Bpu10I CC'TnA_GC
Cuts at: 1071 1088 1839 2622 1071
Size: 17 751 783 4394
Fragments arranged by size:
4394 783 751 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2124 2423 3121 4000 4492 4790 2124
Size: 299 698 879 492 298 3279
Fragments arranged by size:
3279 879 698 492 299 298
BsaI GGTCTCn'nnnn_
Cuts at: 1071 2098 5649 1071
Size: 1027 3551 1367
Fragments arranged by size:
3551 1367 1027
BsaAI yAC'GTr
Cuts at: 3298 3298
Size: 5945
BsaBI GATnn'nnATC
Cuts at: 6 3459 5496 6
Size: 3453 2037 455
Fragments arranged by size:
3453 2037 455
BsaHI Gr'CG_yC
Cuts at: 2539 3376 3624 4326 5822 2539
Size: 837 248 702 1496 2662
Fragments arranged by size:
2662 1496 837 702 248
BsaWI w'CCGG_w
Cuts at: 3134 3655 4333 4607 4754 5165 3134
Size: 521 678 274 147 411 3914
Fragments arranged by size:
3914 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1158 1188 1686 1716 1743 1773 1158
Size: 30 498 30 27 30 5330
Fragments arranged by size:
5330 498 30 30 30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 491 905 1059 1554 1827 491
Size: 414 154 495 273 4609
Fragments arranged by size:
4609 495 414 273 154
BseYI C'CCAG_C
Cuts at: 894 1474 2595 3370 4128 4705 5201 894
Size: 580 1121 775 758 577 496 1638
Fragments arranged by size:
1638 1121 775 758 580 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 629 1124 1292 629
Size: 495 168 5282
Fragments arranged by size:
5282 495 168
BsiEI CG_ry'CG
Cuts at: 104 3487 3533 4741 104
Size: 3383 46 1208 1308
Fragments arranged by size:
3383 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2959 3737 3927 4719 2959
Size: 778 190 792 4185
Fragments arranged by size:
4185 792 778 190
BsmI GAATG_Cn'
Cuts at: 2626 2626
Size: 5945
BsmBI CGTCTCn'nnnn_
Cuts at: 1739 1739
Size: 5945
Bsp1286I G_dGCh'C
Cuts at: 257 1403 1940 2054 2959 3570 3663 3737 3927
Size: 1146 537 114 905 611 93 74 190
Cuts at: 3927 3989 4719 257
Size: 62 730 1483
Fragments arranged by size:
1483 1146 905 730 611 537 190 114
93 74 62
BspEI T'CCGG_A
Cuts at: 5165 5165
Size: 5945
BspHI T'CATG_A
Cuts at: 5121 5121
Size: 5945
BspMI ACCTGCnnnn'nnnn_
Cuts at: 757 948 1037 1053 2808 2928 2995 3209 3511
Size: 191 89 16 1755 120 67 214 302
Cuts at: 3511 3892 757
Size: 381 2810
Fragments arranged by size:
2810 1755 381 302 214 191 120 89
67 16
BsrBI CCG'CTC
Cuts at: 50 2650 4237 4291 5775 5940 50
Size: 2600 1587 54 1484 165 55
Fragments arranged by size:
2600 1587 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 275 1129 1768 2174 3857 275
Size: 854 639 406 1683 2363
Fragments arranged by size:
2363 1683 854 639 406
BsrFI r'CCGG_y
Cuts at: 1483 2665 3943 4124 4333 1483
Size: 1182 1278 181 209 3095
Fragments arranged by size:
3095 1278 1182 209 181
BsrGI T'GTAC_A
Cuts at: 1776 1776
Size: 5945
BssSI C'ACGA_G
Cuts at: 4216 4574 4216
Size: 358 5587
BstAPI GCAn_nnn'nTGC
Cuts at: 742 751 3322 742
Size: 9 2571 3365
Fragments arranged by size:
3365 2571 9
BstBI TT'CG_AA
Cuts at: 853 4306 853
Size: 3453 2492
BstYI r'GATC_y
Cuts at: 1 1878 2970 3437 3460 3795 4041 5053 5139
Size: 1877 1092 467 23 335 246 1012 86
Cuts at: 5139 5151 5497 1
Size: 12 346 449
Fragments arranged by size:
1877 1092 1012 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 1264 1739 107
Size: 1157 475 4313
Fragments arranged by size:
4313 1157 475
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3895 4076 4239 3895
Size: 181 163 5601
Fragments arranged by size:
5601 181 163
BtsI GCAGTG_nn'
Cuts at: 1136 1207 1793 2189 2408 3188 1136
Size: 71 586 396 219 780 3893
Fragments arranged by size:
3893 780 586 396 219 71
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 296 331 2157 2192 296
Size: 35 1826 35 4049
Fragments arranged by size:
4049 1826 35 35
DraI TTT'AAA
Cuts at: 2117 2944 2117
Size: 827 5118
DraIII CAC_nnn'GTG
Cuts at: 751 1495 751
Size: 744 5201
DrdI GACnn_nn'nnGTC
Cuts at: 3651 4509 3651
Size: 858 5087
EaeI y'GGCC_r
Cuts at: 3530 3704 4095 4122 5467 3530
Size: 174 391 27 1345 4008
Fragments arranged by size:
4008 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3530 3530
Size: 5945
EarI CTCTTCn'nnn_
Cuts at: 1347 1625 1641 1919 2201 2519 3968 4178 1347
Size: 278 16 278 282 318 1449 210 3114
Fragments arranged by size:
3114 1449 318 282 278 278 210 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4153 4463 4609 5755 4153
Size: 310 146 1146 4343
Fragments arranged by size:
4343 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2957 2957
Size: 5945
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1606 1628 1688 2082 2351 2585 2867 3770 4202
Size: 22 60 394 269 234 282 903 432
Cuts at: 4202 4949 5217 1606
Size: 747 268 2334
Fragments arranged by size:
2334 903 747 432 394 282 269 268
234 60 22
EcoNI CCTnn'n_nnAGG
Cuts at: 1250 1250
Size: 5945
EcoO109I rG'GnC_Cy
Cuts at: 1216 1255 2443 3084 1216
Size: 39 1188 641 4077
Fragments arranged by size:
4077 1188 641 39
EcoRI G'AATT_C
Cuts at: 402 650 2949 402
Size: 248 2299 3398
Fragments arranged by size:
3398 2299 248
EcoRV GAT'ATC
Cuts at: 515 515
Size: 5945
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3964 3964
Size: 5945
FspI TGC'GCA
Cuts at: 2798 3726 2798
Size: 928 5017
HaeII r_GCGC'y
Cuts at: 2542 2890 3379 3627 4649 5628 2542
Size: 348 489 248 1022 979 2859
Fragments arranged by size:
2859 1022 979 489 348 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 103 135 1686 1718 3787 3817 3819 3849 103
Size: 32 1551 32 2069 30 2 30 2199
Fragments arranged by size:
2199 2069 1551 32 32 30 30 2
HincII GTy'rAC
Cuts at: 904 2984 904
Size: 2080 3865
KasI G'GCGC_C
Cuts at: 2538 3375 3623 2538
Size: 837 248 4860
Fragments arranged by size:
4860 837 248
KpnI G_GTAC'C
Cuts at: 778 1874 2965 778
Size: 1096 1091 3758
Fragments arranged by size:
3758 1096 1091
MfeI C'AATT_G
Cuts at: 182 182
Size: 5945
MluI A'CGCG_T
Cuts at: 5378 5378
Size: 5945
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1463 4616 4800 1463
Size: 3153 184 2608
Fragments arranged by size:
3153 2608 184
MscI TGG'CCA
Cuts at: 3706 3706
Size: 5945
MslI CAynn'nnrTG
Cuts at: 1816 3934 4061 1816
Size: 2118 127 3700
Fragments arranged by size:
3700 2118 127
MspA1I CmG'CkG
Cuts at: 1006 2302 2344 2557 3056 3370 3730 4743 4988
Size: 1296 42 213 499 314 360 1013 245
Cuts at: 4988 1006
Size: 1963
Fragments arranged by size:
1963 1296 1013 499 360 314 245 213
42
NaeI GCC'GGC
Cuts at: 4126 4126
Size: 5945
NarI GG'CG_CC
Cuts at: 2539 3376 3624 2539
Size: 837 248 4860
Fragments arranged by size:
4860 837 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5945
NgoMIV G'CCGG_C
Cuts at: 4124 4124
Size: 5945
NspI r_CATG'y
Cuts at: 736 2998 3498 4405 736
Size: 2262 500 907 2276
Fragments arranged by size:
2276 2262 907 500
PasI CC'CwG_GG
Cuts at: 1057 2272 2737 1057
Size: 1215 465 4265
Fragments arranged by size:
4265 1215 465
PciI A'CATG_T
Cuts at: 4401 4401
Size: 5945
PflMI CCAn_nnn'nTGG
Cuts at: 2158 2158
Size: 5945
PmeI GTTT'AAAC
Cuts at: 2944 2944
Size: 5945
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1618 1650 5084 5116 1618
Size: 32 3434 32 2447
Fragments arranged by size:
3434 2447 32 32
PpuMI rG'GwC_Cy
Cuts at: 1216 1255 2443 1216
Size: 39 1188 4718
Fragments arranged by size:
4718 1188 39
PshAI GACnn'nnGTC
Cuts at: 2448 2448
Size: 5945
PsiI TTA'TAA
Cuts at: 758 758
Size: 5945
PstI C_TGCA'G
Cuts at: 2537 2694 2992 3677 2537
Size: 157 298 685 4805
Fragments arranged by size:
4805 685 298 157
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5945
PvuII CAG'CTG
Cuts at: 1006 2344 2557 3370 3730 1006
Size: 1338 213 813 360 3221
Fragments arranged by size:
3221 1338 813 360 213
RsrII CG'GwC_CG
Cuts at: 4140 4140
Size: 5945
SacI G_AGCT'C
Cuts at: 2959 2959
Size: 5945
SalI G'TCGA_C
Cuts at: 2982 2982
Size: 5945
SapI GCTCTTCn'nnn_
Cuts at: 1347 2201 2519 3968 4178 1347
Size: 854 318 1449 210 3114
Fragments arranged by size:
3114 1449 854 318 210
SbfI CC_TGCA'GG
Cuts at: 2992 2992
Size: 5945
ScaI AGT'ACT
Cuts at: 2109 5462 2109
Size: 3353 2592
SfcI C'TryA_G
Cuts at: 33 827 2533 2546 2690 2988 3673 4666 4857
Size: 794 1706 13 144 298 685 993 191
Cuts at: 4857 33
Size: 1121
Fragments arranged by size:
1706 1121 993 794 685 298 191 144
13
SfoI GGC'GCC
Cuts at: 2540 3377 3625 2540
Size: 837 248 4860
Fragments arranged by size:
4860 837 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5945
SmaI CCC'GGG
Cuts at: 2528 2729 2967 2528
Size: 201 238 5506
Fragments arranged by size:
5506 238 201
SmlI C'TyrA_G
Cuts at: 2103 2438 3100 4015 4507 4769 2103
Size: 335 662 915 492 262 3279
Fragments arranged by size:
3279 915 662 492 335 262
SpeI A'CTAG_T
Cuts at: 67 357 67
Size: 290 5655
SphI G_CATG'C
Cuts at: 2998 3498 2998
Size: 500 5445
StuI AGG'CCT
Cuts at: 1433 1727 2714 1433
Size: 294 987 4664
Fragments arranged by size:
4664 987 294
StyI C'CwwG_G
Cuts at: 107 1428 3079 3433 107
Size: 1321 1651 354 2619
Fragments arranged by size:
2619 1651 1321 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1880 2921 3528 3661 4120 4329 1880
Size: 1041 607 133 459 209 3496
Fragments arranged by size:
3496 1041 607 459 209 133
TatI w'GTAC_w
Cuts at: 619 1162 1776 2107 5460 619
Size: 543 614 331 3353 1104
Fragments arranged by size:
3353 1104 614 543 331
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1164 1196 2181 2213 2400 2432 5462 5494 1164
Size: 32 985 32 187 32 3030 32 1615
Fragments arranged by size:
3030 1615 985 187 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3742 3742
Size: 5945
XbaI T'CTAG_A
Cuts at: 2976 2976
Size: 5945
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1707 3440 1707
Size: 1733 4212
XmaI C'CCGG_G
Cuts at: 2526 2727 2965 2526
Size: 201 238 5506
Fragments arranged by size:
5506 238 201
XmnI GAAnn'nnTTC
Cuts at: 1125 1125
Size: 5945
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BcgI BfrBI BglI BmtI BsiWI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI FseI FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI