Restriction map for
pF1KA2033
(Circular) (Six-base) MAPSORT of: pF1KA2033.seq Check: 1481 from: 1 to: 5351
pF1KA2033 5351 bp
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AatII G_ACGT'C
Cuts at: 955 1485 1963 955
Size: 530 478 4343
Fragments arranged by size:
4343 530 478
AccI GT'mk_AC
Cuts at: 975 1311 1479 1731 1815 1983 2319 2389 975
Size: 336 168 252 84 168 336 70 3937
Fragments arranged by size:
3937 336 336 252 168 168 84 70
Acc65I G'GTAC_C
Cuts at: 2367 2367
Size: 5351
AclI AA'CG_TT
Cuts at: 5191 5191
Size: 5351
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 927 1091 1427 1935 3176 3608 4355 4623 927
Size: 164 336 508 1241 432 747 268 1655
Fragments arranged by size:
1655 1241 747 508 432 336 268 164
AflII C'TTAA_G
Cuts at: 795 795
Size: 5351
AflIII A'CryG_T
Cuts at: 2703 3807 4784 2703
Size: 1104 977 3270
Fragments arranged by size:
3270 1104 977
AgeI A'CCGG_T
Cuts at: 3739 3739
Size: 5351
AhdI GACnn_n'nnGTC
Cuts at: 944 1196 1280 1616 1952 2120 2288 944
Size: 252 84 336 336 168 168 4007
Fragments arranged by size:
4007 336 336 252 168 168 84
AleI CACnn'nnGTG
Cuts at: 902 902
Size: 5351
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 226 258 226
Size: 32 5319
AlwNI CAG_nnn'CTG
Cuts at: 2656 4223 2656
Size: 1567 3784
ApaLI G'TGCA_C
Cuts at: 4121 4121
Size: 5351
ApoI r'AATT_y
Cuts at: 154 400 644 699 1377 1397 1565 1901 2069
Size: 246 244 55 678 20 168 336 168
Cuts at: 2069 2355 4704 4960 154
Size: 286 2349 256 545
Fragments arranged by size:
2349 678 545 336 286 256 246 244
168 168 55 20
AseI AT'TA_AT
Cuts at: 20 20
Size: 5351
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5351
AvaI C'yCGr_G
Cuts at: 2371 2371
Size: 5351
BamHI G'GATC_C
Cuts at: 2376 2376
Size: 5351
BanI G'GyrC_C
Cuts at: 2367 2781 3029 3064 2367
Size: 414 248 35 4654
Fragments arranged by size:
4654 414 248 35
BanII G_rGCy'C
Cuts at: 2365 3395 2365
Size: 1030 4321
BbeI G_GCGC'C
Cuts at: 2785 3033 2785
Size: 248 5103
BbsI GAAGACnn'nnnn_
Cuts at: 1415 1415
Size: 5351
BciVI GTATCCnnnnn_n'
Cuts at: 3249 4010 5351 3249
Size: 761 1341 3249
Fragments arranged by size:
3249 1341 761
BclI T'GATC_A
Cuts at: 486 2871 486
Size: 2385 2966
BglII A'GATC_T
Cuts at: 2866 4903 2866
Size: 2037 3314
BlpI GC'TnA_GC
Cuts at: 2463 2463
Size: 5351
Bme1580I G_kGCm'C
Cuts at: 2976 3069 4125 2976
Size: 93 1056 4202
Fragments arranged by size:
4202 1056 93
BmgBI CAC'GTC
Cuts at: 213 213
Size: 5351
BmrI ACTGGGnnnn_n'
Cuts at: 2563 2644 2979 5123 2563
Size: 81 335 2144 2791
Fragments arranged by size:
2791 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3718 3718
Size: 5351
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 1007 1175 1259 1343 1511 1595 1679 1763
Size: 766 168 84 84 168 84 84 84
Cuts at: 1763 2099 2183 2267 2351 241
Size: 336 84 84 84 3241
Fragments arranged by size:
3241 766 336 168 168 84 84 84
84 84 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2527 3406 3898 4196 2527
Size: 879 492 298 3682
Fragments arranged by size:
3682 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 246 5055 246
Size: 4809 542
BsaAI yAC'GTr
Cuts at: 2704 2704
Size: 5351
BsaBI GATnn'nnATC
Cuts at: 6 2865 4902 6
Size: 2859 2037 455
Fragments arranged by size:
2859 2037 455
BsaHI Gr'CG_yC
Cuts at: 952 1482 1960 2782 3030 3732 5228 952
Size: 530 478 822 248 702 1496 1075
Fragments arranged by size:
1496 1075 822 702 530 478 248
BsaWI w'CCGG_w
Cuts at: 1993 2540 3061 3739 4013 4160 4571 1993
Size: 547 521 678 274 147 411 2773
Fragments arranged by size:
2773 678 547 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 226 256 268 298 226
Size: 30 12 30 5279
Fragments arranged by size:
5279 30 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 181
Size: 5351
BseYI C'CCAG_C
Cuts at: 2776 3534 4111 4607 2776
Size: 758 577 496 3520
Fragments arranged by size:
3520 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1707 1707
Size: 5351
BsiEI CG_ry'CG
Cuts at: 104 2893 2939 4147 104
Size: 2789 46 1208 1308
Fragments arranged by size:
2789 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2365 3143 3333 4125 2365
Size: 778 190 792 3591
Fragments arranged by size:
3591 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 2365 2976 3069 3143 3333 3395 4125 2365
Size: 611 93 74 190 62 730 3591
Fragments arranged by size:
3591 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4571 4571
Size: 5351
BspHI T'CATG_A
Cuts at: 390 4527 390
Size: 4137 1214
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1729 2401 2615 2917 3298 1729
Size: 672 214 302 381 3782
Fragments arranged by size:
3782 672 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3643 3697 5181 5346 50
Size: 3593 54 1484 165 55
Fragments arranged by size:
3593 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 325 3263 325
Size: 2938 2413
BsrFI r'CCGG_y
Cuts at: 3349 3530 3739 3349
Size: 181 209 4961
Fragments arranged by size:
4961 209 181
BssSI C'ACGA_G
Cuts at: 3622 3980 3622
Size: 358 4993
BstAPI GCAn_nnn'nTGC
Cuts at: 2728 2728
Size: 5351
BstBI TT'CG_AA
Cuts at: 3712 3712
Size: 5351
BstYI r'GATC_y
Cuts at: 1 2376 2843 2866 3201 3447 4459 4545 4557
Size: 2375 467 23 335 246 1012 86 12
Cuts at: 4557 4903 1
Size: 346 449
Fragments arranged by size:
2375 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 681 107
Size: 574 4777
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3301 3482 3645 3301
Size: 181 163 5007
Fragments arranged by size:
5007 181 163
BtsI GCAGTG_nn'
Cuts at: 2594 2594
Size: 5351
DraI TTT'AAA
Cuts at: 2350 2350
Size: 5351
DrdI GACnn_nn'nnGTC
Cuts at: 3057 3915 3057
Size: 858 4493
EaeI y'GGCC_r
Cuts at: 146 429 2936 3110 3501 3528 4873 146
Size: 283 2507 174 391 27 1345 624
Fragments arranged by size:
2507 1345 624 391 283 174 27
EagI C'GGCC_G
Cuts at: 2936 2936
Size: 5351
EarI CTCTTCn'nnn_
Cuts at: 261 572 1067 3374 3584 261
Size: 311 495 2307 210 2028
Fragments arranged by size:
2307 2028 495 311 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3559 3869 4015 5161 3559
Size: 310 146 1146 3749
Fragments arranged by size:
3749 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2363 2363
Size: 5351
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 927 1007 1091 1175 1259 1343 1427 1511
Size: 686 80 84 84 84 84 84 84
Cuts at: 1511 1595 1679 1763 1935 2099 2183 2267 2351
Size: 84 84 84 172 164 84 84 84
Cuts at: 2351 3176 3608 4355 4623 241
Size: 825 432 747 268 969
Fragments arranged by size:
969 825 747 686 432 268 172 164
84 84 84 84 84 84 84 84
84 84 84 84 80
EcoO109I rG'GnC_Cy
Cuts at: 2490 2490
Size: 5351
EcoRI G'AATT_C
Cuts at: 154 699 1565 2069 2355 154
Size: 545 866 504 286 3150
Fragments arranged by size:
3150 866 545 504 286
EcoRV GAT'ATC
Cuts at: 252 252
Size: 5351
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 297 541 665 3370 297
Size: 244 124 2705 2278
Fragments arranged by size:
2705 2278 244 124
FspI TGC'GCA
Cuts at: 3132 3132
Size: 5351
HaeII r_GCGC'y
Cuts at: 2785 3033 4055 5034 2785
Size: 248 1022 979 3102
Fragments arranged by size:
3102 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 268 300 930 962 1182 1214 1266 1298 1602
Size: 32 630 32 220 32 52 32 304
Cuts at: 1602 1634 1770 1802 1938 1970 2106 2138 2274
Size: 32 136 32 136 32 136 32 136
Cuts at: 2274 2306 3193 3223 3225 3255 268
Size: 32 887 30 2 30 2364
Fragments arranged by size:
2364 887 630 304 220 136 136 136
136 52 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 287 464 1796 2390 287
Size: 177 1332 594 3248
Fragments arranged by size:
3248 1332 594 177
HindIII A'AGCT_T
Cuts at: 534 1022 1358 1526 1667 1862 2030 534
Size: 488 336 168 141 195 168 3855
Fragments arranged by size:
3855 488 336 195 168 168 141
HpaI GTT'AAC
Cuts at: 1796 1796
Size: 5351
KasI G'GCGC_C
Cuts at: 2781 3029 2781
Size: 248 5103
KpnI G_GTAC'C
Cuts at: 2371 2371
Size: 5351
MluI A'CGCG_T
Cuts at: 4784 4784
Size: 5351
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1600 1684 1936 2104 4022 4206 1600
Size: 84 252 168 1918 184 2745
Fragments arranged by size:
2745 1918 252 184 168 84
MscI TGG'CCA
Cuts at: 148 431 3112 148
Size: 283 2681 2387
Fragments arranged by size:
2681 2387 283
MslI CAynn'nnrTG
Cuts at: 350 395 902 1086 1590 3340 3467 350
Size: 45 507 184 504 1750 127 2234
Fragments arranged by size:
2234 1750 507 504 184 127 45
MspA1I CmG'CkG
Cuts at: 2462 2776 3136 4149 4394 2462
Size: 314 360 1013 245 3419
Fragments arranged by size:
3419 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3532 3532
Size: 5351
NarI GG'CG_CC
Cuts at: 2782 3030 2782
Size: 248 5103
NcoI C'CATG_G
Cuts at: 107 681 107
Size: 574 4777
NgoMIV G'CCGG_C
Cuts at: 3530 3530
Size: 5351
NspI r_CATG'y
Cuts at: 884 968 1220 1304 1472 1640 1976 2312 2404
Size: 84 252 84 168 168 336 336 92
Cuts at: 2404 2904 3811 884
Size: 500 907 2424
Fragments arranged by size:
2424 907 500 336 336 252 168 168
92 84 84
PciI A'CATG_T
Cuts at: 3807 3807
Size: 5351
PmeI GTTT'AAAC
Cuts at: 2350 2350
Size: 5351
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 226 258 4490 4522 226
Size: 32 4232 32 1055
Fragments arranged by size:
4232 1055 32 32
PsiI TTA'TAA
Cuts at: 2177 2177
Size: 5351
PstI C_TGCA'G
Cuts at: 2398 3083 2398
Size: 685 4666
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5351
PvuII CAG'CTG
Cuts at: 2776 3136 2776
Size: 360 4991
RsrII CG'GwC_CG
Cuts at: 3546 3546
Size: 5351
SacI G_AGCT'C
Cuts at: 2365 2365
Size: 5351
SalI G'TCGA_C
Cuts at: 2388 2388
Size: 5351
SapI GCTCTTCn'nnn_
Cuts at: 3374 3584 3374
Size: 210 5141
SbfI CC_TGCA'GG
Cuts at: 2398 2398
Size: 5351
ScaI AGT'ACT
Cuts at: 4868 4868
Size: 5351
SexAI A'CCwGG_T
Cuts at: 236 236
Size: 5351
SfcI C'TryA_G
Cuts at: 33 2394 3079 4072 4263 33
Size: 2361 685 993 191 1121
Fragments arranged by size:
2361 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2783 3031 2783
Size: 248 5103
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5351
SmaI CCC'GGG
Cuts at: 2373 2373
Size: 5351
SmlI C'TyrA_G
Cuts at: 795 2506 3421 3913 4175 795
Size: 1711 915 492 262 1971
Fragments arranged by size:
1971 1711 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5351
SphI G_CATG'C
Cuts at: 884 2404 2904 884
Size: 1520 500 3331
Fragments arranged by size:
3331 1520 500
StyI C'CwwG_G
Cuts at: 107 623 681 2485 2839 107
Size: 516 58 1804 354 2619
Fragments arranged by size:
2619 1804 516 354 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 456 589 2934 3067 3526 3735 456
Size: 133 2345 133 459 209 2072
Fragments arranged by size:
2345 2072 459 209 133 133
TatI w'GTAC_w
Cuts at: 472 729 4866 472
Size: 257 4137 957
Fragments arranged by size:
4137 957 257
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 604 636 1192 1224 1276 1308 4868 4900 604
Size: 32 556 32 52 32 3560 32 1055
Fragments arranged by size:
3560 1055 556 52 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3148 3148
Size: 5351
XbaI T'CTAG_A
Cuts at: 2382 2382
Size: 5351
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2846 2846
Size: 5351
XmaI C'CCGG_G
Cuts at: 2371 2371
Size: 5351
XmnI GAAnn'nnTTC
Cuts at: 277 691 277
Size: 414 4937
ZraI GAC'GTC
Cuts at: 953 1483 1961 953
Size: 530 478 4343
Fragments arranged by size:
4343 530 478
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AlfI ApaI AscI AvrII BaeI BbvCI
BcgI BfrBI BglI BmtI Bpu10I BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI FseI FspAI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI