Restriction map for
pF1KB0001
(Circular) (Six-base) MAPSORT of: pF1KB0001.seq Check: 1644 from: 1 to: 10646
pF1KB0001 10646 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 748 1221 1548 3341 3426 3696 4786 5661 5916
Size: 473 327 1793 85 270 1090 875 255
Cuts at: 5916 6981 748
Size: 1065 4413
Fragments arranged by size:
4413 1793 1090 1065 875 473 327 270
255 85
AatII G_ACGT'C
Cuts at: 6080 6080
Size: 10646
AccI GT'mk_AC
Cuts at: 4622 4805 5060 7684 4622
Size: 183 255 2624 7584
Fragments arranged by size:
7584 2624 255 183
Acc65I G'GTAC_C
Cuts at: 7662 7662
Size: 10646
AclI AA'CG_TT
Cuts at: 10486 10486
Size: 10646
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 540 703 1723 2440 2452 3092 3598 3655
Size: 251 163 1020 717 12 640 506 57
Cuts at: 3655 3841 4300 4411 4456 5485 5722 7297 8471
Size: 186 459 111 45 1029 237 1575 1174
Cuts at: 8471 8903 9650 9918 289
Size: 432 747 268 1017
Fragments arranged by size:
1575 1174 1029 1020 1017 747 717 640
506 459 432 268 251 237 186 163
111 57 45 12
AfeI AGC'GCT
Cuts at: 1211 2005 1211
Size: 794 9852
AflIII A'CryG_T
Cuts at: 4232 7620 7998 9102 10079 4232
Size: 3388 378 1104 977 4799
Fragments arranged by size:
4799 3388 1104 977 378
AgeI A'CCGG_T
Cuts at: 9034 9034
Size: 10646
AleI CACnn'nnGTG
Cuts at: 697 1000 3799 5200 6871 697
Size: 303 2799 1401 1671 4472
Fragments arranged by size:
4472 2799 1671 1401 303
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 665 3998 5693 6451 7453 665
Size: 3333 1695 758 1002 3858
Fragments arranged by size:
3858 3333 1695 1002 758
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6751 6783 6751
Size: 32 10614
AlwNI CAG_nnn'CTG
Cuts at: 1020 1248 1416 1449 1719 3303 3549 3576 3639
Size: 228 168 33 270 1584 246 27 63
Cuts at: 3639 3711 3945 4012 5817 6603 6675 7338 7353
Size: 72 234 67 1805 786 72 663 15
Cuts at: 7353 7951 9518 1020
Size: 598 1567 2148
Fragments arranged by size:
2148 1805 1584 1567 786 663 598 270
246 234 228 168 72 72 67 63
33 27 15
ApaI G_GGCC'C
Cuts at: 566 1170 3609 5610 5964 5999 6793 7009 566
Size: 604 2439 2001 354 35 794 216 4203
Fragments arranged by size:
4203 2439 2001 794 604 354 216 35
ApaLI G'TGCA_C
Cuts at: 1306 5589 6847 6924 9416 1306
Size: 4283 1258 77 2492 2536
Fragments arranged by size:
4283 2536 2492 1258 77
ApoI r'AATT_y
Cuts at: 5419 7650 9999 10255 5419
Size: 2231 2349 256 5810
Fragments arranged by size:
5810 2349 2231 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 10646
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10646
AvaI C'yCGr_G
Cuts at: 478 730 1034 1113 2013 2061 2260 2579 3156
Size: 252 304 79 900 48 199 319 577
Cuts at: 3156 3617 4072 4165 4458 4569 4718 4917 5107
Size: 461 455 93 293 111 149 199 190
Cuts at: 5107 5130 5136 5276 5963 6125 6929 7000 7666
Size: 23 6 140 687 162 804 71 666
Cuts at: 7666 478
Size: 3458
Fragments arranged by size:
3458 900 804 687 666 577 461 455
319 304 293 252 199 199 190 162
149 140 111 93 79 71 48 23
6
BamHI G'GATC_C
Cuts at: 3999 5864 7671 3999
Size: 1865 1807 6974
Fragments arranged by size:
6974 1865 1807
BanI G'GyrC_C
Cuts at: 736 856 994 1097 1560 1960 2022 3438 3485
Size: 120 138 103 463 400 62 1416 47
Cuts at: 3485 3588 4157 4786 4956 5049 5906 6100 6289
Size: 103 569 629 170 93 857 194 189
Cuts at: 6289 7462 7662 8076 8324 8359 736
Size: 1173 200 414 248 35 3023
Fragments arranged by size:
3023 1416 1173 857 629 569 463 414
400 248 200 194 189 170 138 120
103 103 93 62 47 35
BanII G_rGCy'C
Cuts at: 239 566 1041 1163 1170 1300 2201 2332 3053
Size: 327 475 122 7 130 901 131 721
Cuts at: 3053 3229 3398 3609 4499 4576 5137 5610 5964
Size: 176 169 211 890 77 561 473 354
Cuts at: 5964 5999 6617 6793 6826 6865 7009 7087 7660
Size: 35 618 176 33 39 144 78 573
Cuts at: 7660 8690 239
Size: 1030 2195
Fragments arranged by size:
2195 1030 901 890 721 618 573 561
475 473 354 327 211 176 176 169
144 131 130 122 78 77 39 35
33 7
BbeI G_GCGC'C
Cuts at: 1964 4790 5910 6293 7466 8080 8328 1964
Size: 2826 1120 383 1173 614 248 4282
Fragments arranged by size:
4282 2826 1173 1120 614 383 248
BbsI GAAGACnn'nnnn_
Cuts at: 2803 4418 5184 6003 6153 2803
Size: 1615 766 819 150 7296
Fragments arranged by size:
7296 1615 819 766 150
BbvCI CC'TCA_GC
Cuts at: 338 1822 2101 2679 3358 4086 4537 7190 338
Size: 1484 279 578 679 728 451 2653 3794
Fragments arranged by size:
3794 2653 1484 728 679 578 451 279
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 732 766 1025 1059 5247 5281 5500 5534 5646
Size: 34 259 34 4188 34 219 34 112
Cuts at: 5646 5680 6116 6150 6511 6545 732
Size: 34 436 34 361 34 4833
Fragments arranged by size:
4833 4188 436 361 259 219 112 34
34 34 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7533 8544 9305 10646 7533
Size: 1011 761 1341 7533
Fragments arranged by size:
7533 1341 1011 761
BclI T'GATC_A
Cuts at: 7316 8166 7316
Size: 850 9796
BglI GCCn_nnn'nGGC
Cuts at: 625 734 952 2015 3331 3949 4274 4431 4642
Size: 109 218 1063 1316 618 325 157 211
Cuts at: 4642 5983 6269 7045 7334 625
Size: 1341 286 776 289 3937
Fragments arranged by size:
3937 1341 1316 1063 776 618 325 289
286 218 211 157 109
BglII A'GATC_T
Cuts at: 8161 10198 8161
Size: 2037 8609
BlpI GC'TnA_GC
Cuts at: 724 1630 1723 1807 2245 3751 3886 3910 6898
Size: 906 93 84 438 1506 135 24 2988
Cuts at: 6898 7354 7397 7613 7758 724
Size: 456 43 216 145 3612
Fragments arranged by size:
3612 2988 1506 906 456 438 216 145
135 93 84 43 24
Bme1580I G_kGCm'C
Cuts at: 566 649 739 1100 1170 1310 1565 1609 2027
Size: 83 90 361 70 140 255 44 418
Cuts at: 2027 3443 3593 3609 4160 5593 5610 5964 5999
Size: 1416 150 16 551 1433 17 354 35
Cuts at: 5999 6139 6793 6851 6928 7009 8271 8364 9420
Size: 140 654 58 77 81 1262 93 1056
Cuts at: 9420 566
Size: 1792
Fragments arranged by size:
1792 1433 1416 1262 1056 654 551 418
361 354 255 150 140 140 93 90
83 81 77 70 58 44 35 17
16
BmgBI CAC'GTC
Cuts at: 2294 2637 3339 4554 6303 7533 2294
Size: 343 702 1215 1749 1230 5407
Fragments arranged by size:
5407 1749 1230 1215 702 343
BmrI ACTGGGnnnn_n'
Cuts at: 2262 2900 3192 7858 7939 8274 10418 2262
Size: 638 292 4666 81 335 2144 2490
Fragments arranged by size:
4666 2490 2144 638 335 292 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1128 5061 6696 9013 1128
Size: 3933 1635 2317 2761
Fragments arranged by size:
3933 2761 2317 1635
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 579 1171 1441 1941 2404 2506 2926 2941 3154
Size: 592 270 500 463 102 420 15 213
Cuts at: 3154 3469 3715 3967 4236 4684 5053 5215 5821
Size: 315 246 252 269 448 369 162 606
Cuts at: 5821 5974 6067 6403 6625 7279 7582 7597 579
Size: 153 93 336 222 654 303 15 3628
Fragments arranged by size:
3628 654 606 592 500 463 448 420
369 336 315 303 270 269 252 246
222 213 162 153 102 93 15 15
Bpu10I CC'TnA_GC
Cuts at: 338 466 1459 1822 2101 2366 2679 2782 3358
Size: 128 993 363 279 265 313 103 576
Cuts at: 3358 4086 4222 4537 6140 6424 7190 338
Size: 728 136 315 1603 284 766 3794
Fragments arranged by size:
3794 1603 993 766 728 576 363 315
313 284 279 265 136 128 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 729 2185 3763 5569 6051 7822 8701 9193 9491
Size: 1456 1578 1806 482 1771 879 492 298
Cuts at: 9491 729
Size: 1884
Fragments arranged by size:
1884 1806 1771 1578 1456 879 492 482
298
BsaI GGTCTCn'nnnn_
Cuts at: 445 467 4598 10350 445
Size: 22 4131 5752 741
Fragments arranged by size:
5752 4131 741 22
BsaAI yAC'GTr
Cuts at: 2361 7137 7999 2361
Size: 4776 862 5008
Fragments arranged by size:
5008 4776 862
BsaBI GATnn'nnATC
Cuts at: 6 3217 8160 10197 6
Size: 3211 4943 2037 455
Fragments arranged by size:
4943 3211 2037 455
BsaHI Gr'CG_yC
Cuts at: 425 1961 2534 2825 3380 4787 4928 5621 5907
Size: 1536 573 291 555 1407 141 693 286
Cuts at: 5907 6008 6077 6290 6373 7463 8077 8325 9027
Size: 101 69 213 83 1090 614 248 702
Cuts at: 9027 10523 425
Size: 1496 548
Fragments arranged by size:
1536 1496 1407 1090 702 693 614 573
555 548 291 286 248 213 141 101
83 69
BsaWI w'CCGG_w
Cuts at: 4514 4995 5867 6659 7835 8356 9034 9308 9455
Size: 481 872 792 1176 521 678 274 147
Cuts at: 9455 9866 4514
Size: 411 5294
Fragments arranged by size:
5294 1176 872 792 678 521 481 411
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 523 553 2101 2131 2338 2368 2692 2722 3469
Size: 30 1548 30 207 30 324 30 747
Cuts at: 3469 3499 4558 4588 4595 4625 4717 4747 4798
Size: 30 1059 30 7 30 92 30 51
Cuts at: 4798 4828 6641 6671 6751 6781 7483 7513 523
Size: 30 1813 30 80 30 702 30 3656
Fragments arranged by size:
3656 1813 1548 1059 747 702 324 207
92 80 51 30 30 30 30 30
30 30 30 30 30 30 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 582 1912 2005 3493 3529 4681 4696 4743
Size: 455 1330 93 1488 36 1152 15 47
Cuts at: 4743 5050 5698 6156 7303 127
Size: 307 648 458 1147 3470
Fragments arranged by size:
3470 1488 1330 1152 1147 648 458 455
307 93 47 36 15
BseYI C'CCAG_C
Cuts at: 565 714 1206 1524 2571 3566 3814 4018 4376
Size: 149 492 318 1047 995 248 204 358
Cuts at: 4376 4742 4769 4790 5246 6362 6740 7064 7076
Size: 366 27 21 456 1116 378 324 12
Cuts at: 7076 7086 8071 8829 9406 9902 565
Size: 10 985 758 577 496 1309
Fragments arranged by size:
1309 1116 1047 995 985 758 577 496
492 456 378 366 358 324 318 248
204 149 27 21 12 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 1162 1429 1573 2427 2500 2848 3562 4123
Size: 441 267 144 854 73 348 714 561
Cuts at: 4123 4782 5569 5689 5854 5944 6301 6838 7015
Size: 659 787 120 165 90 357 537 177
Cuts at: 7015 7243 721
Size: 228 4124
Fragments arranged by size:
4124 854 787 714 659 561 537 441
357 348 267 228 177 165 144 120
90 73
BsiEI CG_ry'CG
Cuts at: 104 947 2645 2754 3930 5400 6344 8188 8234
Size: 843 1698 109 1176 1470 944 1844 46
Cuts at: 8234 9442 104
Size: 1208 1308
Fragments arranged by size:
1844 1698 1470 1308 1208 1176 944 843
109 46
BsiHKAI G_wGCw'C
Cuts at: 1157 1310 1729 1741 2201 2372 4711 5593 6331
Size: 153 419 12 460 171 2339 882 738
Cuts at: 6331 6617 6826 6851 6928 7063 7247 7660 8438
Size: 286 209 25 77 135 184 413 778
Cuts at: 8438 8628 9420 1157
Size: 190 792 2383
Fragments arranged by size:
2383 2339 882 792 778 738 460 419
413 286 209 190 184 171 153 135
77 25 12
BsmBI CGTCTCn'nnnn_
Cuts at: 4919 5107 6313 7192 4919
Size: 188 1206 879 8373
Fragments arranged by size:
8373 1206 879 188
Bsp1286I G_dGCh'C
Cuts at: 239 566 649 739 1041 1100 1157 1163 1170
Size: 327 83 90 302 59 57 6 7
Cuts at: 1170 1300 1310 1565 1609 1729 1741 2027 2201
Size: 130 10 255 44 120 12 286 174
Cuts at: 2201 2332 2372 3053 3229 3398 3443 3593 3609
Size: 131 40 681 176 169 45 150 16
Cuts at: 3609 4160 4499 4576 4711 5137 5593 5610 5964
Size: 551 339 77 135 426 456 17 354
Cuts at: 5964 5999 6139 6331 6617 6793 6826 6851 6865
Size: 35 140 192 286 176 33 25 14
Cuts at: 6865 6928 7009 7063 7087 7247 7660 8271 8364
Size: 63 81 54 24 160 413 611 93
Cuts at: 8364 8438 8628 8690 9420 239
Size: 74 190 62 730 1465
Fragments arranged by size:
1465 730 681 611 551 456 426 413
354 339 327 302 286 286 255 192
190 176 176 174 169 160 150 140
135 131 130 120 93 90 83 81
77 74 63 62 59 57 54 45
44 40 35 33 25 24 17 16
14 12 10 7 6
BspEI T'CCGG_A
Cuts at: 5867 6659 9866 5867
Size: 792 3207 6647
Fragments arranged by size:
6647 3207 792
BspHI T'CATG_A
Cuts at: 6578 9822 6578
Size: 3244 7402
BspMI ACCTGCnnnn'nnnn_
Cuts at: 748 1184 1221 1548 1811 3257 3341 3426 3695
Size: 436 37 327 263 1446 84 85 269
Cuts at: 3695 3696 4786 5661 5880 5916 6770 6981 7696
Size: 1 1090 875 219 36 854 211 715
Cuts at: 7696 7910 8212 8593 748
Size: 214 302 381 2801
Fragments arranged by size:
2801 1446 1090 875 854 715 436 381
327 302 269 263 219 214 211 85
84 37 36 1
BsrBI CCG'CTC
Cuts at: 50 443 779 929 1581 5445 6831 8938 8992
Size: 393 336 150 652 3864 1386 2107 54
Cuts at: 8992 10476 10641 50
Size: 1484 165 55
Fragments arranged by size:
3864 2107 1484 1386 652 393 336 165
150 55 54
BsrDI GCAATG_nn'
Cuts at: 5972 6195 8558 5972
Size: 223 2363 8060
Fragments arranged by size:
8060 2363 223
BsrFI r'CCGG_y
Cuts at: 127 533 853 2137 2500 2640 3847 4319 4353
Size: 406 320 1284 363 140 1207 472 34
Cuts at: 4353 5554 5897 6251 7021 8644 8825 9034 127
Size: 1201 343 354 770 1623 181 209 1739
Fragments arranged by size:
1739 1623 1284 1207 1201 770 472 406
363 354 343 320 209 181 140 34
BsrGI T'GTAC_A
Cuts at: 3305 3305
Size: 10646
BssHII G'CGCG_C
Cuts at: 3498 7446 3498
Size: 3948 6698
BssSI C'ACGA_G
Cuts at: 487 5842 8917 9275 487
Size: 5355 3075 358 1858
Fragments arranged by size:
5355 3075 1858 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1416 1560 3438 3549 3639 4012 4146 5301 8023
Size: 144 1878 111 90 373 134 1155 2722
Cuts at: 8023 1416
Size: 4039
Fragments arranged by size:
4039 2722 1878 1155 373 144 134 111
90
BstBI TT'CG_AA
Cuts at: 9007 9007
Size: 10646
BstEII G'GTnAC_C
Cuts at: 3561 3856 6120 7543 3561
Size: 295 2264 1423 6664
Fragments arranged by size:
6664 2264 1423 295
BstXI CCAn_nnnn'nTGG
Cuts at: 603 4026 4880 4971 6705 603
Size: 3423 854 91 1734 4544
Fragments arranged by size:
4544 3423 1734 854 91
BstYI r'GATC_y
Cuts at: 1 1202 3611 3999 5864 7671 8138 8161 8496
Size: 1201 2409 388 1865 1807 467 23 335
Cuts at: 8496 8742 9754 9840 9852 10198 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2409 1865 1807 1201 1012 467 449 388
346 335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 900 1318 1400 2069 4679 900
Size: 418 82 669 2610 6867
Fragments arranged by size:
6867 2610 669 418 82
BtgI C'CryG_G
Cuts at: 107 984 1257 1509 1964 1973 3044 3415 3800
Size: 877 273 252 455 9 1071 371 385
Cuts at: 3800 3852 5201 5481 5601 5999 6023 6227 6497
Size: 52 1349 280 120 398 24 204 270
Cuts at: 6497 7307 7425 107
Size: 810 118 3328
Fragments arranged by size:
3328 1349 1071 877 810 455 398 385
371 280 273 270 252 204 120 118
52 24 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 535 555 1461 2160 5145 5151 5757 8596 8777
Size: 20 906 699 2985 6 606 2839 181
Cuts at: 8777 8940 535
Size: 163 2241
Fragments arranged by size:
2985 2839 2241 906 699 606 181 163
20 6
BtsI GCAGTG_nn'
Cuts at: 649 2185 3310 3547 3794 4144 5523 5702 5818
Size: 1536 1125 237 247 350 1379 179 116
Cuts at: 5818 6289 6928 7889 649
Size: 471 639 961 3406
Fragments arranged by size:
3406 1536 1379 1125 961 639 471 350
247 237 179 116
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 7440 7475 7440
Size: 35 10611
DraI TTT'AAA
Cuts at: 7645 7645
Size: 10646
DraIII CAC_nnn'GTG
Cuts at: 1613 2376 1613
Size: 763 9883
DrdI GACnn_nn'nnGTC
Cuts at: 813 2755 6343 8352 9210 813
Size: 1942 3588 2009 858 2249
Fragments arranged by size:
3588 2249 2009 1942 858
EaeI y'GGCC_r
Cuts at: 527 794 944 1130 1842 2498 2642 3230 3371
Size: 267 150 186 712 656 144 588 141
Cuts at: 3371 3845 3849 3920 4209 4841 4973 5729 6020
Size: 474 4 71 289 632 132 756 291
Cuts at: 6020 6275 7019 8231 8405 8796 8823 10168 527
Size: 255 744 1212 174 391 27 1345 1005
Fragments arranged by size:
1345 1212 1005 756 744 712 656 632
588 474 391 291 289 267 255 186
174 150 144 141 132 71 27 4
EagI C'GGCC_G
Cuts at: 944 2642 8231 944
Size: 1698 5589 3359
Fragments arranged by size:
5589 3359 1698
EarI CTCTTCn'nnn_
Cuts at: 2767 3016 3061 4252 4312 4422 5351 6403 7114
Size: 249 45 1191 60 110 929 1052 711
Cuts at: 7114 8669 8879 2767
Size: 1555 210 4534
Fragments arranged by size:
4534 1555 1191 1052 929 711 249 210
110 60 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2289 4477 4639 5368 5884 6213 6571 6703 8854
Size: 2188 162 729 516 329 358 132 2151
Cuts at: 8854 9164 9310 10456 2289
Size: 310 146 1146 2479
Fragments arranged by size:
2479 2188 2151 1146 729 516 358 329
310 162 146 132
EcoICRI GAG'CTC
Cuts at: 2199 6615 6824 7658 2199
Size: 4416 209 834 5187
Fragments arranged by size:
5187 4416 834 209
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 540 579 703 1171 1441 1723 1941 2404
Size: 251 39 124 468 270 282 218 463
Cuts at: 2404 2440 2452 2506 2926 2941 3092 3154 3469
Size: 36 12 54 420 15 151 62 315
Cuts at: 3469 3598 3655 3715 3841 3967 4236 4300 4411
Size: 129 57 60 126 126 269 64 111
Cuts at: 4411 4456 4684 5053 5215 5485 5722 5821 5974
Size: 45 228 369 162 270 237 99 153
Cuts at: 5974 6067 6403 6625 7279 7297 7582 7597 8471
Size: 93 336 222 654 18 285 15 874
Cuts at: 8471 8903 9650 9918 289
Size: 432 747 268 1017
Fragments arranged by size:
1017 874 747 654 468 463 432 420
369 336 315 285 282 270 270 269
268 251 237 228 222 218 162 153
151 129 126 126 124 111 99 93
64 62 60 57 54 45 39 36
18 15 15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 1132 1855 1891 1920 2407 3064 3511 4225 4663
Size: 723 36 29 487 657 447 714 438
Cuts at: 4663 6490 7501 1132
Size: 1827 1011 4277
Fragments arranged by size:
4277 1827 1011 723 714 657 487 447
438 36 29
EcoO109I rG'GnC_Cy
Cuts at: 273 462 562 1167 1428 1446 2065 2073 2349
Size: 189 100 605 261 18 619 8 276
Cuts at: 2349 3605 3621 3741 3942 4077 4634 4725 4968
Size: 1256 16 120 201 135 557 91 243
Cuts at: 4968 5487 5606 5960 5967 6483 6789 6790 7005
Size: 519 119 354 7 516 306 1 215
Cuts at: 7005 7006 7037 7281 7335 7476 7785 273
Size: 1 31 244 54 141 309 3134
Fragments arranged by size:
3134 1256 619 605 557 519 516 354
309 306 276 261 244 243 215 201
189 141 135 120 119 100 91 54
31 18 16 8 7 1 1
EcoRI G'AATT_C
Cuts at: 7650 7650
Size: 10646
EcoRV GAT'ATC
Cuts at: 909 909
Size: 10646
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3477 4305 8665 3477
Size: 828 4360 5458
Fragments arranged by size:
5458 4360 828
FseI GG_CCGG'CC
Cuts at: 3851 3851
Size: 10646
FspI TGC'GCA
Cuts at: 8427 8427
Size: 10646
HaeII r_GCGC'y
Cuts at: 1213 1578 1964 2007 4025 4037 4790 5545 5910
Size: 365 386 43 2018 12 753 755 365
Cuts at: 5910 6293 7267 7466 8080 8328 9350 10329 1213
Size: 383 974 199 614 248 1022 979 1530
Fragments arranged by size:
2018 1530 1022 979 974 755 753 614
386 383 365 365 248 199 43 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 321 353 1649 1681 1919 1951 2692 2724 4069
Size: 32 1296 32 238 32 741 32 1345
Cuts at: 4069 4101 4399 4431 4558 4590 4717 4749 8488
Size: 32 298 32 127 32 127 32 3739
Cuts at: 8488 8518 8520 8550 321
Size: 30 2 30 2417
Fragments arranged by size:
3739 2417 1345 1296 741 298 238 127
127 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2316 5331 5849 6537 7685 2316
Size: 3015 518 688 1148 5277
Fragments arranged by size:
5277 3015 1148 688 518
KasI G'GCGC_C
Cuts at: 1960 4786 5906 6289 7462 8076 8324 1960
Size: 2826 1120 383 1173 614 248 4282
Fragments arranged by size:
4282 2826 1173 1120 614 383 248
KpnI G_GTAC'C
Cuts at: 7666 7666
Size: 10646
MluI A'CGCG_T
Cuts at: 4232 7620 10079 4232
Size: 3388 2459 4799
Fragments arranged by size:
4799 3388 2459
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 134 1112 1196 1325 3473 4556 5045 9317 9501
Size: 978 84 129 2148 1083 489 4272 184
Cuts at: 9501 134
Size: 1279
Fragments arranged by size:
4272 2148 1279 1083 978 489 184 129
84
MscI TGG'CCA
Cuts at: 529 1132 4211 4843 4975 8407 529
Size: 603 3079 632 132 3432 2768
Fragments arranged by size:
3432 3079 2768 632 603 132
MslI CAynn'nnrTG
Cuts at: 697 1000 1498 3799 5200 6313 6583 6799 6871
Size: 303 498 2301 1401 1113 270 216 72
Cuts at: 6871 7237 8635 8762 697
Size: 366 1398 127 2581
Fragments arranged by size:
2581 2301 1401 1398 1113 498 366 303
270 216 127 72
MspA1I CmG'CkG
Cuts at: 342 638 760 1065 1388 1567 1716 2100 2240
Size: 296 122 305 323 179 149 384 140
Cuts at: 2240 2835 3417 3693 3755 3909 4376 4742 4934
Size: 595 582 276 62 154 467 366 192
Cuts at: 4934 6098 6480 6756 7068 7275 7396 7571 7757
Size: 1164 382 276 312 207 121 175 186
Cuts at: 7757 8071 8431 9444 9689 342
Size: 314 360 1013 245 1299
Fragments arranged by size:
1299 1164 1013 595 582 467 384 382
366 360 323 314 312 305 296 276
276 245 207 192 186 179 175 154
149 140 122 121 62
NaeI GCC'GGC
Cuts at: 129 855 2502 2642 3849 4321 5556 6253 7023
Size: 726 1647 140 1207 472 1235 697 770
Cuts at: 7023 8827 129
Size: 1804 1948
Fragments arranged by size:
1948 1804 1647 1235 1207 770 726 697
472 140
NarI GG'CG_CC
Cuts at: 1961 4787 5907 6290 7463 8077 8325 1961
Size: 2826 1120 383 1173 614 248 4282
Fragments arranged by size:
4282 2826 1173 1120 614 383 248
NcoI C'CATG_G
Cuts at: 107 1964 5601 6227 7425 107
Size: 1857 3637 626 1198 3328
Fragments arranged by size:
3637 3328 1857 1198 626
NdeI CA'TA_TG
Cuts at: 7210 7210
Size: 10646
NgoMIV G'CCGG_C
Cuts at: 127 853 2500 2640 3847 4319 5554 6251 7021
Size: 726 1647 140 1207 472 1235 697 770
Cuts at: 7021 8825 127
Size: 1804 1948
Fragments arranged by size:
1948 1804 1647 1235 1207 770 726 697
472 140
NspI r_CATG'y
Cuts at: 135 1360 1467 3813 4699 5302 6713 7699 8199
Size: 1225 107 2346 886 603 1411 986 500
Cuts at: 8199 9106 135
Size: 907 1675
Fragments arranged by size:
2346 1675 1411 1225 986 907 886 603
500 107
PasI CC'CwG_GG
Cuts at: 1851 2814 3987 4026 4873 1851
Size: 963 1173 39 847 7624
Fragments arranged by size:
7624 1173 963 847 39
PciI A'CATG_T
Cuts at: 9102 9102
Size: 10646
PflMI CCAn_nnn'nTGG
Cuts at: 1449 1851 2625 2730 3945 4742 6369 6603 6885
Size: 402 774 105 1215 797 1627 234 282
Cuts at: 6885 6960 7338 1449
Size: 75 378 4757
Fragments arranged by size:
4757 1627 1215 797 774 402 378 282
234 105 75
PfoI T'CCnGG_A
Cuts at: 136 911 2213 2342 2918 3131 3446 3761 4556
Size: 775 1302 129 576 213 315 315 795
Cuts at: 4556 5405 5561 5860 6071 136
Size: 849 156 299 211 4711
Fragments arranged by size:
4711 1302 849 795 775 576 315 315
299 213 211 156 129
PmeI GTTT'AAAC
Cuts at: 7645 7645
Size: 10646
PmlI CAC'GTG
Cuts at: 2361 2361
Size: 10646
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6751 6783 9785 9817 6751
Size: 32 3002 32 7580
Fragments arranged by size:
7580 3002 32 32
PpuMI rG'GwC_Cy
Cuts at: 273 1446 2065 3621 3741 4077 4725 5487 6483
Size: 1173 619 1556 120 336 648 762 996
Cuts at: 6483 7476 273
Size: 993 3443
Fragments arranged by size:
3443 1556 1173 996 993 762 648 619
336 120
PspOMI G'GGCC_C
Cuts at: 562 1166 3605 5606 5960 5995 6789 7005 562
Size: 604 2439 2001 354 35 794 216 4203
Fragments arranged by size:
4203 2439 2001 794 604 354 216 35
PstI C_TGCA'G
Cuts at: 614 1232 2195 2411 3212 3317 3512 3554 3667
Size: 618 963 216 801 105 195 42 113
Cuts at: 3667 3692 3908 3971 4589 5369 6671 6923 7073
Size: 25 216 63 618 780 1302 252 150
Cuts at: 7073 7693 8378 614
Size: 620 685 2882
Fragments arranged by size:
2882 1302 963 801 780 685 620 618
618 252 216 216 195 150 113 105
63 42 25
PvuI CG_AT'CG
Cuts at: 104 6344 104
Size: 6240 4406
PvuII CAG'CTG
Cuts at: 342 1716 2100 2835 3693 3909 6756 7068 8071
Size: 1374 384 735 858 216 2847 312 1003
Cuts at: 8071 8431 342
Size: 360 2557
Fragments arranged by size:
2847 2557 1374 1003 858 735 384 360
312 216
RsrII CG'GwC_CG
Cuts at: 257 2135 8841 257
Size: 1878 6706 2062
Fragments arranged by size:
6706 2062 1878
SacI G_AGCT'C
Cuts at: 2201 6617 6826 7660 2201
Size: 4416 209 834 5187
Fragments arranged by size:
5187 4416 834 209
SacII CC_GC'GG
Cuts at: 3418 3418
Size: 10646
SalI G'TCGA_C
Cuts at: 7683 7683
Size: 10646
SanDI GG'GwC_CC
Cuts at: 3621 4077 3621
Size: 456 10190
SapI GCTCTTCn'nnn_
Cuts at: 3016 4312 8669 8879 3016
Size: 1296 4357 210 4783
Fragments arranged by size:
4783 4357 1296 210
SbfI CC_TGCA'GG
Cuts at: 3512 3667 7693 3512
Size: 155 4026 6465
Fragments arranged by size:
6465 4026 155
ScaI AGT'ACT
Cuts at: 10163 10163
Size: 10646
SexAI A'CCwGG_T
Cuts at: 2321 2729 2960 4607 5333 6485 6620 2321
Size: 408 231 1647 726 1152 135 6347
Fragments arranged by size:
6347 1647 1152 726 408 231 135
SfcI C'TryA_G
Cuts at: 33 495 610 1228 2191 2407 3208 3313 3508
Size: 462 115 618 963 216 801 105 195
Cuts at: 3508 3550 3663 3688 3904 3967 4585 5061 5365
Size: 42 113 25 216 63 618 476 304
Cuts at: 5365 6459 6667 6919 7069 7689 8374 9367 9558
Size: 1094 208 252 150 620 685 993 191
Cuts at: 9558 33
Size: 1121
Fragments arranged by size:
1121 1094 993 963 801 685 620 618
618 476 462 304 252 216 216 208
195 191 150 115 113 105 63 42
25
SfiI GGCCn_nnn'nGGCC
Cuts at: 952 6269 7045 952
Size: 5317 776 4553
Fragments arranged by size:
5317 4553 776
SfoI GGC'GCC
Cuts at: 1962 4788 5908 6291 7464 8078 8326 1962
Size: 2826 1120 383 1173 614 248 4282
Fragments arranged by size:
4282 2826 1173 1120 614 383 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10646
SgrAI Cr'CCGG_yG
Cuts at: 5897 5897
Size: 10646
SmaI CCC'GGG
Cuts at: 2015 3619 4571 4720 5109 5278 5965 7668 2015
Size: 1604 952 149 389 169 687 1703 4993
Fragments arranged by size:
4993 1703 1604 952 687 389 169 149
SmlI C'TyrA_G
Cuts at: 708 2200 3778 5584 6030 7801 8716 9208 9470
Size: 1492 1578 1806 446 1771 915 492 262
Cuts at: 9470 708
Size: 1884
Fragments arranged by size:
1884 1806 1771 1578 1492 915 492 446
262
SnaBI TAC'GTA
Cuts at: 7137 7137
Size: 10646
SpeI A'CTAG_T
Cuts at: 67 67
Size: 10646
SphI G_CATG'C
Cuts at: 1467 6713 7699 8199 1467
Size: 5246 986 500 3914
Fragments arranged by size:
5246 3914 986 500
StuI AGG'CCT
Cuts at: 748 1793 2926 3784 3881 6263 6272 748
Size: 1045 1133 858 97 2382 9 5122
Fragments arranged by size:
5122 2382 1133 1045 858 97 9
StyI C'CwwG_G
Cuts at: 107 998 1133 1964 2042 2123 3557 4964 5348
Size: 891 135 831 78 81 1434 1407 384
Cuts at: 5348 5601 5640 6227 6722 7412 7425 7780 8134
Size: 253 39 587 495 690 13 355 354
Cuts at: 8134 107
Size: 2619
Fragments arranged by size:
2619 1434 1407 891 831 690 587 495
384 355 354 253 135 81 78 39
13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 420 1516 3174 3304 4368 5776 6866 7111 7165
Size: 1096 1658 130 1064 1408 1090 245 54
Cuts at: 7165 7425 8229 8362 8821 9030 420
Size: 260 804 133 459 209 2036
Fragments arranged by size:
2036 1658 1408 1096 1090 1064 804 459
260 245 209 133 130 54
TatI w'GTAC_w
Cuts at: 2306 3305 6092 7403 10161 2306
Size: 999 2787 1311 2758 2791
Fragments arranged by size:
2791 2787 2758 1311 999
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2097 2129 2338 2370 3469 3501 3850 3882 4595
Size: 32 209 32 1099 32 349 32 713
Cuts at: 4595 4627 4985 5017 10163 10195 2097
Size: 32 358 32 5146 32 2548
Fragments arranged by size:
5146 2548 1099 713 358 349 209 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 202 1753 2050 2898 5335 8443 202
Size: 1551 297 848 2437 3108 2405
Fragments arranged by size:
3108 2437 2405 1551 848 297
XbaI T'CTAG_A
Cuts at: 7677 7677
Size: 10646
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 573 816 1021 1089 1971 2609 4461 5028
Size: 459 243 205 68 882 638 1852 567
Cuts at: 5028 6225 6300 7137 8141 114
Size: 1197 75 837 1004 2619
Fragments arranged by size:
2619 1852 1197 1004 882 837 638 567
459 243 205 75 68
XmaI C'CCGG_G
Cuts at: 2013 3617 4569 4718 5107 5276 5963 7666 2013
Size: 1604 952 149 389 169 687 1703 4993
Fragments arranged by size:
4993 1703 1604 952 687 389 169 149
XmnI GAAnn'nnTTC
Cuts at: 1693 4288 1693
Size: 2595 8051
ZraI GAC'GTC
Cuts at: 6078 6078
Size: 10646
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AscI AvrII BaeI BfrBI BmtI BsiWI
BsmI BstZ17I ClaI FspAI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PshAI PsiI PspXI PsrI
SrfI SspI SwaI XhoI