Restriction map for
pF1KB0009
(Circular) (Six-base) MAPSORT of: pF1KB0009.seq Check: 3622 from: 1 to: 8075
pF1KB0009 8075 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 713 998 1485 1737 713
Size: 285 487 252 7051
Fragments arranged by size:
7051 487 285 252
AccI GT'mk_AC
Cuts at: 5113 5113
Size: 8075
Acc65I G'GTAC_C
Cuts at: 5091 5091
Size: 8075
AclI AA'CG_TT
Cuts at: 7915 7915
Size: 8075
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 841 2071 2819 3079 4375 4607 4846 5900 6332
Size: 1230 748 260 1296 232 239 1054 432
Cuts at: 6332 7079 7347 841
Size: 747 268 1569
Fragments arranged by size:
1569 1296 1230 1054 748 747 432 268
260 239 232
AfeI AGC'GCT
Cuts at: 3495 3495
Size: 8075
AflII C'TTAA_G
Cuts at: 3268 3268
Size: 8075
AflIII A'CryG_T
Cuts at: 371 2030 5427 6531 7508 371
Size: 1659 3397 1104 977 938
Fragments arranged by size:
3397 1659 1104 977 938
AgeI A'CCGG_T
Cuts at: 6463 6463
Size: 8075
AhdI GACnn_n'nnGTC
Cuts at: 4539 4539
Size: 8075
AleI CACnn'nnGTG
Cuts at: 2942 2942
Size: 8075
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2960 2960
Size: 8075
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1567 1599 2362 2394 1567
Size: 32 763 32 7248
Fragments arranged by size:
7248 763 32 32
AlwNI CAG_nnn'CTG
Cuts at: 861 936 990 1389 1629 1713 2122 2142 2538
Size: 75 54 399 240 84 409 20 396
Cuts at: 2538 2610 2814 3588 5380 6947 861
Size: 72 204 774 1792 1567 1989
Fragments arranged by size:
1989 1792 1567 774 409 399 396 240
204 84 75 72 54 20
ApaI G_GGCC'C
Cuts at: 1833 1833
Size: 8075
ApaLI G'TGCA_C
Cuts at: 700 1618 6845 700
Size: 918 5227 1930
Fragments arranged by size:
5227 1930 918
ApoI r'AATT_y
Cuts at: 544 2704 3532 3607 3928 4183 4708 5079 7428
Size: 2160 828 75 321 255 525 371 2349
Cuts at: 7428 7684 544
Size: 256 935
Fragments arranged by size:
2349 2160 935 828 525 371 321 256
255 75
AseI AT'TA_AT
Cuts at: 20 20
Size: 8075
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8075
AvaI C'yCGr_G
Cuts at: 718 912 1826 1942 2251 3362 3370 5095 718
Size: 194 914 116 309 1111 8 1725 3698
Fragments arranged by size:
3698 1725 1111 914 309 194 116 8
AvrII C'CTAG_G
Cuts at: 3792 3792
Size: 8075
BamHI G'GATC_C
Cuts at: 2822 3515 4043 5100 2822
Size: 693 528 1057 5797
Fragments arranged by size:
5797 1057 693 528
BanI G'GyrC_C
Cuts at: 939 3615 4252 5033 5091 5505 5753 5788 939
Size: 2676 637 781 58 414 248 35 3226
Fragments arranged by size:
3226 2676 781 637 414 248 58 35
BanII G_rGCy'C
Cuts at: 415 775 1570 1827 1833 2261 2738 3404 4397
Size: 360 795 257 6 428 477 666 993
Cuts at: 4397 5089 6119 415
Size: 692 1030 2371
Fragments arranged by size:
2371 1030 993 795 692 666 477 428
360 257 6
BbeI G_GCGC'C
Cuts at: 5509 5757 5509
Size: 248 7827
BbsI GAAGACnn'nnnn_
Cuts at: 329 529 1719 2087 2219 2483 2938 3074 329
Size: 200 1190 368 132 264 455 136 5330
Fragments arranged by size:
5330 1190 455 368 264 200 136 132
BbvCI CC'TCA_GC
Cuts at: 3299 3299
Size: 8075
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1042 1076 1329 1363 2986 3020 3833 3867 1042
Size: 34 253 34 1623 34 813 34 5250
Fragments arranged by size:
5250 1623 813 253 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5973 6734 8075 5973
Size: 761 1341 5973
Fragments arranged by size:
5973 1341 761
BclI T'GATC_A
Cuts at: 2144 5595 2144
Size: 3451 4624
BfrBI ATG'CAT
Cuts at: 4452 4452
Size: 8075
BglI GCCn_nnn'nGGC
Cuts at: 1529 3097 1529
Size: 1568 6507
BglII A'GATC_T
Cuts at: 1280 1949 4085 4586 5590 7627 1280
Size: 669 2136 501 1004 2037 1728
Fragments arranged by size:
2136 2037 1728 1004 669 501
BlpI GC'TnA_GC
Cuts at: 1379 3497 5187 1379
Size: 2118 1690 4267
Fragments arranged by size:
4267 2118 1690
Bme1580I G_kGCm'C
Cuts at: 704 944 1622 1833 2949 4255 5700 5793 6849
Size: 240 678 211 1116 1306 1445 93 1056
Cuts at: 6849 704
Size: 1930
Fragments arranged by size:
1930 1445 1306 1116 1056 678 240 211
93
BmgBI CAC'GTC
Cuts at: 372 1635 372
Size: 1263 6812
BmrI ACTGGGnnnn_n'
Cuts at: 3338 5287 5368 5703 7847 3338
Size: 1949 81 335 2144 3566
Fragments arranged by size:
3566 2144 1949 335 81
BmtI G_CTAG'C
Cuts at: 742 2566 742
Size: 1824 6251
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6442 6442
Size: 8075
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 712 1093 1141 1690 1820 1993 2776 2965
Size: 15 381 48 549 130 173 783 189
Cuts at: 2965 3157 3581 3802 3844 3934 4144 4261 4429
Size: 192 424 221 42 90 210 117 168
Cuts at: 4429 4495 5066 697
Size: 66 571 3706
Fragments arranged by size:
3706 783 571 549 424 381 221 210
192 189 173 168 130 117 90 66
48 42 15
Bpu10I CC'TnA_GC
Cuts at: 1564 2230 3299 1564
Size: 666 1069 6340
Fragments arranged by size:
6340 1069 666
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 790 1003 3295 4381 5251 6130 6622 6920 790
Size: 213 2292 1086 870 879 492 298 1945
Fragments arranged by size:
2292 1945 1086 879 870 492 298 213
BsaI GGTCTCn'nnnn_
Cuts at: 3559 3988 7779 3559
Size: 429 3791 3855
Fragments arranged by size:
3855 3791 429
BsaAI yAC'GTr
Cuts at: 1877 4689 5428 1877
Size: 2812 739 4524
Fragments arranged by size:
4524 2812 739
BsaBI GATnn'nnATC
Cuts at: 6 5589 7626 6
Size: 5583 2037 455
Fragments arranged by size:
5583 2037 455
BsaHI Gr'CG_yC
Cuts at: 2465 3878 5506 5754 6456 7952 2465
Size: 1413 1628 248 702 1496 2588
Fragments arranged by size:
2588 1628 1496 1413 702 248
BsaWI w'CCGG_w
Cuts at: 3158 4550 5264 5785 6463 6737 6884 7295 3158
Size: 1392 714 521 678 274 147 411 3938
Fragments arranged by size:
3938 1392 714 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2296 2326 2362 2392 2774 2804 4850 4880 4948
Size: 30 36 30 382 30 2046 30 68
Cuts at: 4948 4978 2296
Size: 30 5393
Fragments arranged by size:
5393 2046 382 68 36 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 430 631 664 694 850 1960 2386 3589 4042
Size: 201 33 30 156 1110 426 1203 453
Cuts at: 4042 430
Size: 4463
Fragments arranged by size:
4463 1203 1110 453 426 201 156 33
30
BseYI C'CCAG_C
Cuts at: 605 1289 2260 3195 3840 4671 5500 6258 6835
Size: 684 971 935 645 831 829 758 577
Cuts at: 6835 7331 605
Size: 496 1349
Fragments arranged by size:
1349 971 935 831 829 758 684 645
577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 955 976 1440 2135 2383 2596 3261 4513 955
Size: 21 464 695 248 213 665 1252 4517
Fragments arranged by size:
4517 1252 695 665 464 248 213 21
BsiEI CG_ry'CG
Cuts at: 104 5617 5663 6871 104
Size: 5513 46 1208 1308
Fragments arranged by size:
5513 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 704 1622 2738 2906 3404 4397 4688 5089 5867
Size: 918 1116 168 498 993 291 401 778
Cuts at: 5867 6057 6849 704
Size: 190 792 1930
Fragments arranged by size:
1930 1116 993 918 792 778 498 401
291 190 168
BsmI GAATG_Cn'
Cuts at: 1200 3300 1200
Size: 2100 5975
BsmBI CGTCTCn'nnnn_
Cuts at: 550 550
Size: 8075
Bsp1286I G_dGCh'C
Cuts at: 415 704 775 944 1570 1622 1827 1833 2261
Size: 289 71 169 626 52 205 6 428
Cuts at: 2261 2738 2906 2949 3404 4255 4397 4688 5089
Size: 477 168 43 455 851 142 291 401
Cuts at: 5089 5700 5793 5867 6057 6119 6849 415
Size: 611 93 74 190 62 730 1641
Fragments arranged by size:
1641 851 730 626 611 477 455 428
401 291 289 205 190 169 168 142
93 74 71 62 52 43 6
BspEI T'CCGG_A
Cuts at: 4550 7295 4550
Size: 2745 5330
BspHI T'CATG_A
Cuts at: 303 7251 303
Size: 6948 1127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 335 713 998 1485 1737 2943 5125 5339 5641
Size: 378 285 487 252 1206 2182 214 302
Cuts at: 5641 6022 335
Size: 381 2388
Fragments arranged by size:
2388 2182 1206 487 381 378 302 285
252 214
BsrBI CCG'CTC
Cuts at: 50 2388 4566 6367 6421 7905 8070 50
Size: 2338 2178 1801 54 1484 165 55
Fragments arranged by size:
2338 2178 1801 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2835 4967 5987 2835
Size: 2132 1020 4923
Fragments arranged by size:
4923 2132 1020
BsrFI r'CCGG_y
Cuts at: 1249 2986 3071 6073 6254 6463 1249
Size: 1737 85 3002 181 209 2861
Fragments arranged by size:
3002 2861 1737 209 181 85
BsrGI T'GTAC_A
Cuts at: 1740 1740
Size: 8075
BssSI C'ACGA_G
Cuts at: 337 2383 2905 6346 6704 337
Size: 2046 522 3441 358 1708
Fragments arranged by size:
3441 2046 1708 522 358
BstAPI GCAn_nnn'nTGC
Cuts at: 861 878 990 1418 1626 3054 5452 861
Size: 17 112 428 208 1428 2398 3484
Fragments arranged by size:
3484 2398 1428 428 208 112 17
BstBI TT'CG_AA
Cuts at: 6436 6436
Size: 8075
BstEII G'GTnAC_C
Cuts at: 1497 3717 1497
Size: 2220 5855
BstXI CCAn_nnnn'nTGG
Cuts at: 960 1293 4023 4939 960
Size: 333 2730 916 4096
Fragments arranged by size:
4096 2730 916 333
BstYI r'GATC_y
Cuts at: 1 473 1280 1949 2607 2822 3515 4031 4043
Size: 472 807 669 658 215 693 516 12
Cuts at: 4043 4085 4586 5049 5100 5567 5590 5925 6171
Size: 42 501 463 51 467 23 335 246
Cuts at: 6171 7183 7269 7281 7627 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 807 693 669 658 516 501 472
467 463 449 346 335 246 215 86
51 42 23 12 12
Bsu36I CC'TnA_GG
Cuts at: 3754 4145 3754
Size: 391 7684
BtgI C'CryG_G
Cuts at: 1226 1770 3698 3722 1226
Size: 544 1928 24 5579
Fragments arranged by size:
5579 1928 544 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6025 6206 6369 6025
Size: 181 163 7731
Fragments arranged by size:
7731 181 163
BtsI GCAGTG_nn'
Cuts at: 874 924 1428 1622 2776 4158 5318 874
Size: 50 504 194 1154 1382 1160 3631
Fragments arranged by size:
3631 1382 1160 1154 504 194 50
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 575 610 5049 5084 575
Size: 35 4439 35 3566
Fragments arranged by size:
4439 3566 35 35
DraI TTT'AAA
Cuts at: 4850 5074 4850
Size: 224 7851
DraIII CAC_nnn'GTG
Cuts at: 1626 1626
Size: 8075
DrdI GACnn_nn'nnGTC
Cuts at: 5781 6639 5781
Size: 858 7217
EaeI y'GGCC_r
Cuts at: 350 1592 2921 2988 3662 4289 5660 5834 6225
Size: 1242 1329 67 674 627 1371 174 391
Cuts at: 6225 6252 7597 350
Size: 27 1345 828
Fragments arranged by size:
1371 1345 1329 1242 828 674 627 391
174 67 27
EagI C'GGCC_G
Cuts at: 5660 5660
Size: 8075
EarI CTCTTCn'nnn_
Cuts at: 1054 1303 1834 1853 3893 4308 6098 6308 1054
Size: 249 531 19 2040 415 1790 210 2821
Fragments arranged by size:
2821 2040 1790 531 415 249 210 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 284 3306 6283 6593 6739 7885 284
Size: 3022 2977 310 146 1146 474
Fragments arranged by size:
3022 2977 1146 474 310 146
EcoICRI GAG'CTC
Cuts at: 2736 3402 4395 5087 2736
Size: 666 993 692 5724
Fragments arranged by size:
5724 993 692 666
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 712 841 1093 1141 1690 1820 1993 2071
Size: 15 129 252 48 549 130 173 78
Cuts at: 2071 2776 2819 2965 3079 3157 3581 3802 3844
Size: 705 43 146 114 78 424 221 42
Cuts at: 3844 3934 4144 4261 4375 4429 4495 4607 4846
Size: 90 210 117 114 54 66 112 239
Cuts at: 4846 5066 5900 6332 7079 7347 697
Size: 220 834 432 747 268 1425
Fragments arranged by size:
1425 834 747 705 549 432 424 268
252 239 221 220 210 173 146 130
129 117 114 114 112 90 78 78
66 54 48 43 42 15
EcoNI CCTnn'n_nnAGG
Cuts at: 1243 2089 1243
Size: 846 7229
EcoO109I rG'GnC_Cy
Cuts at: 423 579 723 825 1830 2514 3367 3819 4533
Size: 156 144 102 1005 684 853 452 714
Cuts at: 4533 4979 5041 5214 423
Size: 446 62 173 3284
Fragments arranged by size:
3284 1005 853 714 684 452 446 173
156 144 102 62
EcoRI G'AATT_C
Cuts at: 3607 5079 3607
Size: 1472 6603
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 255 546 1558 2500 3279 6094 255
Size: 291 1012 942 779 2815 2236
Fragments arranged by size:
2815 2236 1012 942 779 291
FseI GG_CCGG'CC
Cuts at: 2990 2990
Size: 8075
FspI TGC'GCA
Cuts at: 1012 1981 1999 2422 5856 1012
Size: 969 18 423 3434 3231
Fragments arranged by size:
3434 3231 969 423 18
FspAI rTGC'GCAy
Cuts at: 1981 1981
Size: 8075
HaeII r_GCGC'y
Cuts at: 698 2242 2547 3497 3508 5509 5757 6779 7758
Size: 1544 305 950 11 2001 248 1022 979
Cuts at: 7758 698
Size: 1015
Fragments arranged by size:
2001 1544 1022 1015 979 950 305 248
11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 160 192 877 909 939 971 2160 2192 3402
Size: 32 685 32 30 32 1189 32 1210
Cuts at: 3402 3434 4525 4557 4948 4980 5041 5073 5917
Size: 32 1091 32 391 32 61 32 844
Cuts at: 5917 5947 5949 5979 160
Size: 30 2 30 2256
Fragments arranged by size:
2256 1210 1189 1091 844 685 391 61
32 32 32 32 32 32 32 32
30 30 30 2
HincII GTy'rAC
Cuts at: 1206 3801 5114 1206
Size: 2595 1313 4167
Fragments arranged by size:
4167 2595 1313
HindIII A'AGCT_T
Cuts at: 200 2017 3646 200
Size: 1817 1629 4629
Fragments arranged by size:
4629 1817 1629
KasI G'GCGC_C
Cuts at: 5505 5753 5505
Size: 248 7827
KpnI G_GTAC'C
Cuts at: 5095 5095
Size: 8075
MluI A'CGCG_T
Cuts at: 7508 7508
Size: 8075
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1733 2081 2312 2858 2940 4250 4793 6746 6930
Size: 348 231 546 82 1310 543 1953 184
Cuts at: 6930 1733
Size: 2878
Fragments arranged by size:
2878 1953 1310 546 543 348 231 184
82
MscI TGG'CCA
Cuts at: 352 2923 4291 5836 352
Size: 2571 1368 1545 2591
Fragments arranged by size:
2591 2571 1545 1368
MslI CAynn'nnrTG
Cuts at: 403 1225 2446 2942 2996 3692 6064 6191 403
Size: 822 1221 496 54 696 2372 127 2287
Fragments arranged by size:
2372 2287 1221 822 696 496 127 54
MspA1I CmG'CkG
Cuts at: 469 609 858 878 1041 1293 1557 1710 2013
Size: 140 249 20 163 252 264 153 303
Cuts at: 2013 2535 2892 2898 3660 4278 5186 5500 5860
Size: 522 357 6 762 618 908 314 360
Cuts at: 5860 6873 7118 469
Size: 1013 245 1426
Fragments arranged by size:
1426 1013 908 762 618 522 360 357
314 303 264 252 249 245 163 153
140 20 6
NaeI GCC'GGC
Cuts at: 2988 6256 2988
Size: 3268 4807
NarI GG'CG_CC
Cuts at: 5506 5754 5506
Size: 248 7827
NcoI C'CATG_G
Cuts at: 1770 1770
Size: 8075
NgoMIV G'CCGG_C
Cuts at: 2986 6254 2986
Size: 3268 4807
NheI G'CTAG_C
Cuts at: 738 2562 738
Size: 1824 6251
NsiI A_TGCA'T
Cuts at: 4454 4454
Size: 8075
NspI r_CATG'y
Cuts at: 408 1009 2034 3342 4249 4456 4464 5128 5628
Size: 601 1025 1308 907 207 8 664 500
Cuts at: 5628 6535 408
Size: 907 1948
Fragments arranged by size:
1948 1308 1025 907 907 664 601 500
207 8
PasI CC'CwG_GG
Cuts at: 1570 4039 1570
Size: 2469 5606
PciI A'CATG_T
Cuts at: 2030 6531 2030
Size: 4501 3574
PflMI CCAn_nnn'nTGG
Cuts at: 936 1629 936
Size: 693 7382
PfoI T'CCnGG_A
Cuts at: 728 959 2024 2135 3011 3134 4238 4511 728
Size: 231 1065 111 876 123 1104 273 4292
Fragments arranged by size:
4292 1104 1065 876 273 231 123 111
PmeI GTTT'AAAC
Cuts at: 5074 5074
Size: 8075
PmlI CAC'GTG
Cuts at: 4689 4689
Size: 8075
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 653 685 2362 2394 7214 7246 653
Size: 32 1677 32 4820 32 1482
Fragments arranged by size:
4820 1677 1482 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 423 723 2514 3367 3819 4533 4979 5041 423
Size: 300 1791 853 452 714 446 62 3457
Fragments arranged by size:
3457 1791 853 714 452 446 300 62
PspOMI G'GGCC_C
Cuts at: 1829 1829
Size: 8075
PspXI vC'TCGA_Gb
Cuts at: 718 718
Size: 8075
PstI C_TGCA'G
Cuts at: 455 869 883 1556 1718 1838 2342 2543 2897
Size: 414 14 673 162 120 504 201 354
Cuts at: 2897 2954 3410 5122 5807 455
Size: 57 456 1712 685 2723
Fragments arranged by size:
2723 1712 685 673 504 456 414 354
201 162 120 57 14
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8075
PvuII CAG'CTG
Cuts at: 469 609 1041 1293 2892 3660 5500 5860 469
Size: 140 432 252 1599 768 1840 360 2684
Fragments arranged by size:
2684 1840 1599 768 432 360 252 140
RsrII CG'GwC_CG
Cuts at: 114 6270 114
Size: 6156 1919
SacI G_AGCT'C
Cuts at: 2738 3404 4397 5089 2738
Size: 666 993 692 5724
Fragments arranged by size:
5724 993 692 666
SalI G'TCGA_C
Cuts at: 5112 5112
Size: 8075
SanDI GG'GwC_CC
Cuts at: 3367 4533 3367
Size: 1166 6909
SapI GCTCTTCn'nnn_
Cuts at: 1054 1303 6098 6308 1054
Size: 249 4795 210 2821
Fragments arranged by size:
4795 2821 249 210
SbfI CC_TGCA'GG
Cuts at: 2954 5122 2954
Size: 2168 5907
ScaI AGT'ACT
Cuts at: 7592 7592
Size: 8075
SexAI A'CCwGG_T
Cuts at: 1622 1622
Size: 8075
SfcI C'TryA_G
Cuts at: 33 451 865 879 1552 1714 1834 2338 2539
Size: 418 414 14 673 162 120 504 201
Cuts at: 2539 2893 2950 3406 3738 5118 5803 6796 6987
Size: 354 57 456 332 1380 685 993 191
Cuts at: 6987 33
Size: 1121
Fragments arranged by size:
1380 1121 993 685 673 504 456 418
414 354 332 201 191 162 120 57
14
SfoI GGC'GCC
Cuts at: 5507 5755 5507
Size: 248 7827
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8075
SmaI CCC'GGG
Cuts at: 914 1828 5097 914
Size: 914 3269 3892
Fragments arranged by size:
3892 3269 914
SmlI C'TyrA_G
Cuts at: 718 805 1018 3268 3310 4396 5230 6145 6637
Size: 87 213 2250 42 1086 834 915 492
Cuts at: 6637 6899 718
Size: 262 1894
Fragments arranged by size:
2250 1894 1086 915 834 492 262 213
87 42
SnaBI TAC'GTA
Cuts at: 1877 1877
Size: 8075
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8075
SphI G_CATG'C
Cuts at: 3342 4249 4464 5128 5628 3342
Size: 907 215 664 500 5789
Fragments arranged by size:
5789 907 664 500 215
SrfI GCCC'GGGC
Cuts at: 1828 1828
Size: 8075
StuI AGG'CCT
Cuts at: 1780 3262 3274 1780
Size: 1482 12 6581
Fragments arranged by size:
6581 1482 12
StyI C'CwwG_G
Cuts at: 1610 1770 3792 4592 5209 5563 1610
Size: 160 2022 800 617 354 4122
Fragments arranged by size:
4122 2022 800 617 354 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1303 2322 3705 4663 4696 5658 5791 6250 6459
Size: 1019 1383 958 33 962 133 459 209
Cuts at: 6459 1303
Size: 2919
Fragments arranged by size:
2919 1383 1019 962 958 459 209 133
33
TatI w'GTAC_w
Cuts at: 1740 2435 2618 3314 7590 1740
Size: 695 183 696 4276 2225
Fragments arranged by size:
4276 2225 696 695 183
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1288 1320 1393 1425 2296 2328 4850 4882 7592
Size: 32 73 32 871 32 2522 32 2710
Cuts at: 7592 7624 1288
Size: 32 1739
Fragments arranged by size:
2710 2522 1739 871 73 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3220 5872 3220
Size: 2652 5423
XbaI T'CTAG_A
Cuts at: 2178 5106 2178
Size: 2928 5147
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5570 5570
Size: 8075
XhoI C'TCGA_G
Cuts at: 718 718
Size: 8075
XmaI C'CCGG_G
Cuts at: 912 1826 5095 912
Size: 914 3269 3892
Fragments arranged by size:
3892 3269 914
XmnI GAAnn'nnTTC
Cuts at: 461 2084 2500 4814 461
Size: 1623 416 2314 3722
Fragments arranged by size:
3722 2314 1623 416
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI FspAI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AscI BaeI BsiWI BssHII BstZ17I ClaI EcoRV
HpaI MfeI NdeI NotI NruI PacI PshAI PsiI
PsrI SacII SfiI SgrAI SspI SwaI ZraI