Restriction map for
pF1KB0011
(Circular) (Six-base) MAPSORT of: pF1KB0011.seq Check: 4009 from: 1 to: 4553
pF1KB0011 4553 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 1591 1591
Size: 4553
Acc65I G'GTAC_C
Cuts at: 1569 1569
Size: 4553
AclI AA'CG_TT
Cuts at: 4393 4393
Size: 4553
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1293 2378 2810 3557 3825 1293
Size: 1085 432 747 268 2021
Fragments arranged by size:
2021 1085 747 432 268
AflII C'TTAA_G
Cuts at: 1330 1330
Size: 4553
AflIII A'CryG_T
Cuts at: 1905 3009 3986 1905
Size: 1104 977 2472
Fragments arranged by size:
2472 1104 977
AgeI A'CCGG_T
Cuts at: 2941 2941
Size: 4553
AhdI GACnn_n'nnGTC
Cuts at: 157 157
Size: 4553
AlwNI CAG_nnn'CTG
Cuts at: 268 402 421 1858 3425 268
Size: 134 19 1437 1567 1396
Fragments arranged by size:
1567 1437 1396 134 19
ApaI G_GGCC'C
Cuts at: 570 691 707 755 953 570
Size: 121 16 48 198 4170
Fragments arranged by size:
4170 198 121 48 16
ApaLI G'TGCA_C
Cuts at: 3323 3323
Size: 4553
ApoI r'AATT_y
Cuts at: 1557 3906 4162 1557
Size: 2349 256 1948
Fragments arranged by size:
2349 1948 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4553
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4553
AvaI C'yCGr_G
Cuts at: 129 138 162 183 410 521 740 809 985
Size: 9 24 21 227 111 219 69 176
Cuts at: 985 1573 129
Size: 588 3109
Fragments arranged by size:
3109 588 227 219 176 111 69 24
21 9
AvrII C'CTAG_G
Cuts at: 822 822
Size: 4553
BamHI G'GATC_C
Cuts at: 1578 1578
Size: 4553
BanI G'GyrC_C
Cuts at: 294 922 1569 1983 2231 2266 294
Size: 628 647 414 248 35 2581
Fragments arranged by size:
2581 647 628 414 248 35
BanII G_rGCy'C
Cuts at: 261 570 691 707 747 755 766 953 998
Size: 309 121 16 40 8 11 187 45
Cuts at: 998 1136 1453 1567 2597 261
Size: 138 317 114 1030 2217
Fragments arranged by size:
2217 1030 317 309 187 138 121 114
45 40 16 11 8
BbeI G_GCGC'C
Cuts at: 1987 2235 1987
Size: 248 4305
BbsI GAAGACnn'nnnn_
Cuts at: 1151 1151
Size: 4553
BciVI GTATCCnnnnn_n'
Cuts at: 2451 3212 4553 2451
Size: 761 1341 2451
Fragments arranged by size:
2451 1341 761
BclI T'GATC_A
Cuts at: 2073 2073
Size: 4553
BglI GCCn_nnn'nGGC
Cuts at: 476 913 1089 476
Size: 437 176 3940
Fragments arranged by size:
3940 437 176
BglII A'GATC_T
Cuts at: 2068 4105 2068
Size: 2037 2516
BlpI GC'TnA_GC
Cuts at: 1665 1665
Size: 4553
Bme1580I G_kGCm'C
Cuts at: 299 570 691 707 755 927 953 2178 2271
Size: 271 121 16 48 172 26 1225 93
Cuts at: 2271 3327 299
Size: 1056 1525
Fragments arranged by size:
1525 1225 1056 271 172 121 93 48
26 16
BmgBI CAC'GTC
Cuts at: 1503 1503
Size: 4553
BmrI ACTGGGnnnn_n'
Cuts at: 1765 1846 2181 4325 1765
Size: 81 335 2144 1993
Fragments arranged by size:
2144 1993 335 81
BmtI G_CTAG'C
Cuts at: 896 896
Size: 4553
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 803 2920 803
Size: 2117 2436
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 424 731 904 1249 245
Size: 179 307 173 345 3549
Fragments arranged by size:
3549 345 307 179 173
Bpu10I CC'TnA_GC
Cuts at: 992 992
Size: 4553
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1141 1729 2608 3100 3398 1141
Size: 588 879 492 298 2296
Fragments arranged by size:
2296 879 588 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4257 4257
Size: 4553
BsaAI yAC'GTr
Cuts at: 600 1413 1906 600
Size: 813 493 3247
Fragments arranged by size:
3247 813 493
BsaBI GATnn'nnATC
Cuts at: 6 2067 4104 6
Size: 2061 2037 455
Fragments arranged by size:
2061 2037 455
BsaHI Gr'CG_yC
Cuts at: 1984 2232 2934 4430 1984
Size: 248 702 1496 2107
Fragments arranged by size:
2107 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1250 1490 1742 2263 2941 3215 3362 3773 1250
Size: 240 252 521 678 274 147 411 2030
Fragments arranged by size:
2030 678 521 411 274 252 240 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 140 170 140
Size: 30 4523
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 618 901 618
Size: 283 4270
BseYI C'CCAG_C
Cuts at: 112 339 759 816 877 927 952 1208 1219
Size: 227 420 57 61 50 25 256 11
Cuts at: 1219 1402 1536 1978 2736 3313 3809 112
Size: 183 134 442 758 577 496 856
Fragments arranged by size:
856 758 577 496 442 420 256 227
183 134 61 57 50 25 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 758 164
Size: 594 3959
BsiEI CG_ry'CG
Cuts at: 104 2095 2141 3349 104
Size: 1991 46 1208 1308
Fragments arranged by size:
1991 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1567 2345 2535 3327 1567
Size: 778 190 792 2793
Fragments arranged by size:
2793 792 778 190
BsmI GAATG_Cn'
Cuts at: 1046 1184 1046
Size: 138 4415
Bsp1286I G_dGCh'C
Cuts at: 261 299 570 691 707 747 755 766 927
Size: 38 271 121 16 40 8 11 161
Cuts at: 927 953 998 1136 1453 1567 2178 2271 2345
Size: 26 45 138 317 114 611 93 74
Cuts at: 2345 2535 2597 3327 261
Size: 190 62 730 1487
Fragments arranged by size:
1487 730 611 317 271 190 161 138
121 114 93 74 62 45 40 38
26 16 11 8
BspEI T'CCGG_A
Cuts at: 1250 3773 1250
Size: 2523 2030
BspHI T'CATG_A
Cuts at: 3729 3729
Size: 4553
BspMI ACCTGCnnnn'nnnn_
Cuts at: 324 378 1603 1817 2119 2500 324
Size: 54 1225 214 302 381 2377
Fragments arranged by size:
2377 1225 381 302 214 54
BsrBI CCG'CTC
Cuts at: 50 622 2845 2899 4383 4548 50
Size: 572 2223 54 1484 165 55
Fragments arranged by size:
2223 1484 572 165 55 54
BsrDI GCAATG_nn'
Cuts at: 979 1281 2465 979
Size: 302 1184 3067
Fragments arranged by size:
3067 1184 302
BsrFI r'CCGG_y
Cuts at: 640 2551 2732 2941 640
Size: 1911 181 209 2252
Fragments arranged by size:
2252 1911 209 181
BssHII G'CGCG_C
Cuts at: 1416 1416
Size: 4553
BssSI C'ACGA_G
Cuts at: 682 2824 3182 682
Size: 2142 358 2053
Fragments arranged by size:
2142 2053 358
BstAPI GCAn_nnn'nTGC
Cuts at: 421 922 1930 421
Size: 501 1008 3044
Fragments arranged by size:
3044 1008 501
BstBI TT'CG_AA
Cuts at: 2914 2914
Size: 4553
BstXI CCAn_nnnn'nTGG
Cuts at: 1227 1227
Size: 4553
BstYI r'GATC_y
Cuts at: 1 1253 1266 1578 2045 2068 2403 2649 3661
Size: 1252 13 312 467 23 335 246 1012
Cuts at: 3661 3747 3759 4105 1
Size: 86 12 346 449
Fragments arranged by size:
1252 1012 467 449 346 335 312 246
86 23 13 12
BtgI C'CryG_G
Cuts at: 107 155 308 443 767 908 1023 107
Size: 48 153 135 324 141 115 3637
Fragments arranged by size:
3637 324 153 141 135 115 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 343 2503 2684 2847 343
Size: 2160 181 163 2049
Fragments arranged by size:
2160 2049 181 163
BtsI GCAGTG_nn'
Cuts at: 866 1796 866
Size: 930 3623
DraI TTT'AAA
Cuts at: 1552 1552
Size: 4553
DrdI GACnn_nn'nnGTC
Cuts at: 2259 3117 2259
Size: 858 3695
EaeI y'GGCC_r
Cuts at: 905 971 1090 2138 2312 2703 2730 4075 905
Size: 66 119 1048 174 391 27 1345 1383
Fragments arranged by size:
1383 1345 1048 391 174 119 66 27
EagI C'GGCC_G
Cuts at: 2138 2138
Size: 4553
EarI CTCTTCn'nnn_
Cuts at: 788 848 2576 2786 788
Size: 60 1728 210 2555
Fragments arranged by size:
2555 1728 210 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 238 346 2761 3071 3217 4363 238
Size: 108 2415 310 146 1146 428
Fragments arranged by size:
2415 1146 428 310 146 108
EcoICRI GAG'CTC
Cuts at: 1565 1565
Size: 4553
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 424 731 904 1249 1293 2378 2810 3557
Size: 179 307 173 345 44 1085 432 747
Cuts at: 3557 3825 245
Size: 268 973
Fragments arranged by size:
1085 973 747 432 345 307 268 179
173 44
EcoNI CCTnn'n_nnAGG
Cuts at: 464 464
Size: 4553
EcoO109I rG'GnC_Cy
Cuts at: 151 166 336 469 566 567 678 688 704
Size: 15 170 133 97 1 111 10 16
Cuts at: 704 751 752 782 1125 1215 1398 1692 151
Size: 47 1 30 343 90 183 294 3012
Fragments arranged by size:
3012 343 294 183 170 133 111 97
90 47 30 16 15 10 1 1
EcoRI G'AATT_C
Cuts at: 1557 1557
Size: 4553
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2572 2572
Size: 4553
FspI TGC'GCA
Cuts at: 1045 2334 1045
Size: 1289 3264
HaeII r_GCGC'y
Cuts at: 176 1987 2235 3257 4236 176
Size: 1811 248 1022 979 493
Fragments arranged by size:
1811 1022 979 493 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 143 175 2395 2425 2427 2457 143
Size: 32 2220 30 2 30 2239
Fragments arranged by size:
2239 2220 32 30 30 2
HincII GTy'rAC
Cuts at: 1592 1592
Size: 4553
HindIII A'AGCT_T
Cuts at: 1465 1465
Size: 4553
KasI G'GCGC_C
Cuts at: 1983 2231 1983
Size: 248 4305
KpnI G_GTAC'C
Cuts at: 1573 1573
Size: 4553
MluI A'CGCG_T
Cuts at: 3986 3986
Size: 4553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 992 3224 3408 992
Size: 2232 184 2137
Fragments arranged by size:
2232 2137 184
MscI TGG'CCA
Cuts at: 973 2314 973
Size: 1341 3212
MslI CAynn'nnrTG
Cuts at: 2542 2669 2542
Size: 127 4426
MspA1I CmG'CkG
Cuts at: 343 418 550 759 931 963 1025 1041 1195
Size: 75 132 209 172 32 62 16 154
Cuts at: 1195 1208 1664 1978 2338 3351 3596 343
Size: 13 456 314 360 1013 245 1300
Fragments arranged by size:
1300 1013 456 360 314 245 209 172
154 132 75 62 32 16 13
NaeI GCC'GGC
Cuts at: 642 2734 642
Size: 2092 2461
NarI GG'CG_CC
Cuts at: 1984 2232 1984
Size: 248 4305
NcoI C'CATG_G
Cuts at: 107 443 767 107
Size: 336 324 3893
Fragments arranged by size:
3893 336 324
NgoMIV G'CCGG_C
Cuts at: 640 2732 640
Size: 2092 2461
NheI G'CTAG_C
Cuts at: 892 892
Size: 4553
NruI TCG'CGA
Cuts at: 370 370
Size: 4553
NspI r_CATG'y
Cuts at: 1482 1606 2106 3013 1482
Size: 124 500 907 3022
Fragments arranged by size:
3022 907 500 124
PasI CC'CwG_GG
Cuts at: 1137 1137
Size: 4553
PciI A'CATG_T
Cuts at: 3009 3009
Size: 4553
PflMI CCAn_nnn'nTGG
Cuts at: 268 268
Size: 4553
PmeI GTTT'AAAC
Cuts at: 1552 1552
Size: 4553
PmlI CAC'GTG
Cuts at: 600 600
Size: 4553
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3692 3724 3692
Size: 32 4521
PpuMI rG'GwC_Cy
Cuts at: 151 166 336 1125 1215 1398 151
Size: 15 170 789 90 183 3306
Fragments arranged by size:
3306 789 183 170 90 15
PspOMI G'GGCC_C
Cuts at: 566 687 703 751 949 566
Size: 121 16 48 198 4170
Fragments arranged by size:
4170 198 121 48 16
PstI C_TGCA'G
Cuts at: 426 636 942 1600 2285 426
Size: 210 306 658 685 2694
Fragments arranged by size:
2694 685 658 306 210
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4553
PvuII CAG'CTG
Cuts at: 418 550 931 1208 1978 2338 418
Size: 132 381 277 770 360 2633
Fragments arranged by size:
2633 770 381 360 277 132
RsrII CG'GwC_CG
Cuts at: 2748 2748
Size: 4553
SacI G_AGCT'C
Cuts at: 1567 1567
Size: 4553
SacII CC_GC'GG
Cuts at: 1026 1026
Size: 4553
SalI G'TCGA_C
Cuts at: 1590 1590
Size: 4553
SapI GCTCTTCn'nnn_
Cuts at: 788 2576 2786 788
Size: 1788 210 2555
Fragments arranged by size:
2555 1788 210
SbfI CC_TGCA'GG
Cuts at: 1600 1600
Size: 4553
ScaI AGT'ACT
Cuts at: 4070 4070
Size: 4553
SfcI C'TryA_G
Cuts at: 33 422 604 632 938 1596 2281 3274 3465
Size: 389 182 28 306 658 685 993 191
Cuts at: 3465 33
Size: 1121
Fragments arranged by size:
1121 993 685 658 389 306 191 182
28
SfiI GGCCn_nnn'nGGCC
Cuts at: 1089 1089
Size: 4553
SfoI GGC'GCC
Cuts at: 1985 2233 1985
Size: 248 4305
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4553
SmaI CCC'GGG
Cuts at: 131 140 164 185 1575 131
Size: 9 24 21 1390 3109
Fragments arranged by size:
3109 1390 24 21 9
SmlI C'TyrA_G
Cuts at: 521 1120 1330 1708 2623 3115 3377 521
Size: 599 210 378 915 492 262 1697
Fragments arranged by size:
1697 915 599 492 378 262 210
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4553
SphI G_CATG'C
Cuts at: 1606 2106 1606
Size: 500 4053
StuI AGG'CCT
Cuts at: 827 1109 827
Size: 282 4271
StyI C'CwwG_G
Cuts at: 107 443 767 822 1337 1370 1687 2041 107
Size: 336 324 55 515 33 317 354 2619
Fragments arranged by size:
2619 515 354 336 324 317 55 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 439 732 1129 2136 2269 2728 2937 439
Size: 293 397 1007 133 459 209 2055
Fragments arranged by size:
2055 1007 459 397 293 209 133
TatI w'GTAC_w
Cuts at: 4068 4068
Size: 4553
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 140 172 420 452 1245 1277 4070 4102 140
Size: 32 248 32 793 32 2793 32 591
Fragments arranged by size:
2793 793 591 248 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1504 2350 1504
Size: 846 3707
XbaI T'CTAG_A
Cuts at: 1584 1584
Size: 4553
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 960 2048 960
Size: 1088 3465
XhoI C'TCGA_G
Cuts at: 521 521
Size: 4553
XmaI C'CCGG_G
Cuts at: 129 138 162 183 1573 129
Size: 9 24 21 1390 3109
Fragments arranged by size:
3109 1390 24 21 9
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NruI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AleI AlfI AloI AscI BaeI
BbvCI BcgI BfrBI BsiWI BsmBI BsrGI BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NdeI NotI NsiI PacI PfoI PshAI PsiI
PspXI PsrI SanDI SexAI SgrAI SnaBI SrfI SspI
SwaI XmnI ZraI