Restriction map for
pF1KB0013
(Circular) (Six-base) MAPSORT of: pF1KB0013.seq Check: 3546 from: 1 to: 9584
pF1KB0013 9584 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 170 2567 3230 3298 4501 6353 6414 170
Size: 2397 663 68 1203 1852 61 3340
Fragments arranged by size:
3340 2397 1852 1203 663 68 61
AatII G_ACGT'C
Cuts at: 248 4187 5717 6112 248
Size: 3939 1530 395 3720
Fragments arranged by size:
3939 3720 1530 395
AccI GT'mk_AC
Cuts at: 4337 5213 6622 4337
Size: 876 1409 7299
Fragments arranged by size:
7299 1409 876
Acc65I G'GTAC_C
Cuts at: 4004 6600 4004
Size: 2596 6988
AclI AA'CG_TT
Cuts at: 9424 9424
Size: 9584
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 1202 1258 1453 1723 2947 3222 3821 5440
Size: 586 56 195 270 1224 275 599 1619
Cuts at: 5440 7409 7841 8588 8856 616
Size: 1969 432 747 268 1344
Fragments arranged by size:
1969 1619 1344 1224 747 599 586 432
275 270 268 195 56
AflIII A'CryG_T
Cuts at: 635 747 2786 5006 5271 6936 8040 9017 635
Size: 112 2039 2220 265 1665 1104 977 1202
Fragments arranged by size:
2220 2039 1665 1202 1104 977 265 112
AgeI A'CCGG_T
Cuts at: 7972 7972
Size: 9584
AhdI GACnn_n'nnGTC
Cuts at: 1419 2496 1419
Size: 1077 8507
AleI CACnn'nnGTG
Cuts at: 5413 5734 5413
Size: 321 9263
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1328 1659 2542 4997 5537 1328
Size: 331 883 2455 540 5375
Fragments arranged by size:
5375 2455 883 540 331
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4594 4626 4594
Size: 32 9552
AlwNI CAG_nnn'CTG
Cuts at: 729 1431 1926 2724 3207 3313 4101 4890 5355
Size: 702 495 798 483 106 788 789 465
Cuts at: 5355 5430 6889 8456 729
Size: 75 1459 1567 1857
Fragments arranged by size:
1857 1567 1459 798 789 788 702 495
483 465 106 75
ApaI G_GGCC'C
Cuts at: 6170 6170
Size: 9584
ApaLI G'TGCA_C
Cuts at: 361 5128 8354 361
Size: 4767 3226 1591
Fragments arranged by size:
4767 3226 1591
ApoI r'AATT_y
Cuts at: 1000 1162 6588 8937 9193 1000
Size: 162 5426 2349 256 1391
Fragments arranged by size:
5426 2349 1391 256 162
AscI GG'CGCG_CC
Cuts at: 6197 6197
Size: 9584
AseI AT'TA_AT
Cuts at: 20 20
Size: 9584
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9584
AvaI C'yCGr_G
Cuts at: 132 571 819 1402 3913 3922 4323 5336 5443
Size: 439 248 583 2511 9 401 1013 107
Cuts at: 5443 5611 5921 6207 6368 6448 6561 6604 132
Size: 168 310 286 161 80 113 43 3112
Fragments arranged by size:
3112 2511 1013 583 439 401 310 286
248 168 161 113 107 80 43 9
BamHI G'GATC_C
Cuts at: 6014 6531 6609 6014
Size: 517 78 8989
Fragments arranged by size:
8989 517 78
BanI G'GyrC_C
Cuts at: 283 506 1975 2263 3173 3218 3392 4004 5241
Size: 223 1469 288 910 45 174 612 1237
Cuts at: 5241 6357 6364 6392 6472 6600 7014 7262 7297
Size: 1116 7 28 80 128 414 248 35
Cuts at: 7297 283
Size: 2570
Fragments arranged by size:
2570 1469 1237 1116 910 612 414 288
248 223 174 128 80 45 35 28
7
BanII G_rGCy'C
Cuts at: 2129 2648 3835 4330 4640 5345 5450 5914 6082
Size: 519 1187 495 310 705 105 464 168
Cuts at: 6082 6170 6214 6485 6598 7628 2129
Size: 88 44 271 113 1030 4085
Fragments arranged by size:
4085 1187 1030 705 519 495 464 310
271 168 113 105 88 44
BbeI G_GCGC'C
Cuts at: 510 6396 6476 7018 7266 510
Size: 5886 80 542 248 2828
Fragments arranged by size:
5886 2828 542 248 80
BbsI GAAGACnn'nnnn_
Cuts at: 744 1688 2913 2917 3487 3689 3820 4699 6283
Size: 944 1225 4 570 202 131 879 1584
Cuts at: 6283 744
Size: 4045
Fragments arranged by size:
4045 1584 1225 944 879 570 202 131
4
BbvCI CC'TCA_GC
Cuts at: 883 1519 3314 4108 883
Size: 636 1795 794 6359
Fragments arranged by size:
6359 1795 794 636
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 113 147 2224 2258 113
Size: 34 2077 34 7439
Fragments arranged by size:
7439 2077 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7482 8243 9584 7482
Size: 761 1341 7482
Fragments arranged by size:
7482 1341 761
BclI T'GATC_A
Cuts at: 2345 5747 7104 2345
Size: 3402 1357 4825
Fragments arranged by size:
4825 3402 1357
BglI GCCn_nnn'nGGC
Cuts at: 769 893 2230 2239 3983 4132 4403 5201 6373
Size: 124 1337 9 1744 149 271 798 1172
Cuts at: 6373 769
Size: 3980
Fragments arranged by size:
3980 1744 1337 1172 798 271 149 124
9
BglII A'GATC_T
Cuts at: 629 7099 9136 629
Size: 6470 2037 1077
Fragments arranged by size:
6470 2037 1077
BlpI GC'TnA_GC
Cuts at: 3359 4090 4217 6696 3359
Size: 731 127 2479 6247
Fragments arranged by size:
6247 2479 731 127
Bme1580I G_kGCm'C
Cuts at: 365 393 1978 2268 3221 3395 3630 4439 5006
Size: 28 1585 290 953 174 235 809 567
Cuts at: 5006 5132 5246 6170 6360 6369 7209 7302 8358
Size: 126 114 924 190 9 840 93 1056
Cuts at: 8358 365
Size: 1591
Fragments arranged by size:
1591 1585 1056 953 924 840 809 567
290 235 190 174 126 114 93 28
9
BmgBI CAC'GTC
Cuts at: 750 768 1626 3582 750
Size: 18 858 1956 6752
Fragments arranged by size:
6752 1956 858 18
BmrI ACTGGGnnnn_n'
Cuts at: 2589 2641 3818 5881 6379 6796 6877 7212 9356
Size: 52 1177 2063 498 417 81 335 2144
Cuts at: 9356 2589
Size: 2817
Fragments arranged by size:
2817 2144 2063 1177 498 417 335 81
52
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 849 1656 2874 3465 4970 7951 849
Size: 807 1218 591 1505 2981 2482
Fragments arranged by size:
2981 2482 1505 1218 807 591
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 760 964 969 1792 2383 2401 2764 2890 3040
Size: 204 5 823 591 18 363 126 150
Cuts at: 3040 3115 3124 3362 3595 4420 4438 5275 5404
Size: 75 9 238 233 825 18 837 129
Cuts at: 5404 5560 5593 5821 5965 5977 6154 6499 760
Size: 156 33 228 144 12 177 345 3845
Fragments arranged by size:
3845 837 825 823 591 363 345 238
233 228 204 177 156 150 144 129
126 75 33 18 18 12 9 5
Bpu10I CC'TnA_GC
Cuts at: 787 883 1519 3314 4108 787
Size: 96 636 1795 794 6263
Fragments arranged by size:
6263 1795 794 636 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 1156 1243 4624 5704 6760 7639 8131 8429
Size: 567 87 3381 1080 1056 879 492 298
Cuts at: 8429 589
Size: 1744
Fragments arranged by size:
3381 1744 1080 1056 879 567 492 298
87
BsaI GGTCTCn'nnnn_
Cuts at: 2740 3801 3910 9288 2740
Size: 1061 109 5378 3036
Fragments arranged by size:
5378 3036 1061 109
BsaAI yAC'GTr
Cuts at: 468 638 705 1805 2118 4440 4785 5007 5274
Size: 170 67 1100 313 2322 345 222 267
Cuts at: 5274 5732 6937 468
Size: 458 1205 3115
Fragments arranged by size:
3115 2322 1205 1100 458 345 313 267
222 170 67
BsaBI GATnn'nnATC
Cuts at: 6 5746 7098 9135 6
Size: 5740 1352 2037 455
Fragments arranged by size:
5740 2037 1352 455
BsaHI Gr'CG_yC
Cuts at: 245 257 344 507 908 2214 4013 4184 4696
Size: 12 87 163 401 1306 1799 171 512
Cuts at: 4696 5714 6109 6393 6473 7015 7263 7965 9461
Size: 1018 395 284 80 542 248 702 1496
Cuts at: 9461 245
Size: 368
Fragments arranged by size:
1799 1496 1306 1018 702 542 512 401
395 368 284 248 171 163 87 80
12
BsaWI w'CCGG_w
Cuts at: 2759 3008 3092 4486 6773 7294 7972 8246 8393
Size: 249 84 1394 2287 521 678 274 147
Cuts at: 8393 8804 2759
Size: 411 3539
Fragments arranged by size:
3539 2287 1394 678 521 411 274 249
147 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 967 997 1034 1064 4282 4312 5551 5581 5651
Size: 30 37 30 3218 30 1239 30 70
Cuts at: 5651 5681 967
Size: 30 4870
Fragments arranged by size:
4870 3218 1239 70 37 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1055 1600 1906 2309 3331 3438 3634 4168 4186
Size: 545 306 403 1022 107 196 534 18
Cuts at: 4186 4426 5800 5950 5953 5974 5977 6046 6103
Size: 240 1374 150 3 21 3 69 57
Cuts at: 6103 6151 1055
Size: 48 4488
Fragments arranged by size:
4488 1374 1022 545 534 403 306 240
196 150 107 69 57 48 21 18
3 3
BseYI C'CCAG_C
Cuts at: 176 729 2159 2837 3532 6353 7009 7767 8344
Size: 553 1430 678 695 2821 656 758 577
Cuts at: 8344 8840 176
Size: 496 920
Fragments arranged by size:
2821 1430 920 758 695 678 656 577
553 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 2512 2539 2545 2947 3436 6487 148
Size: 2364 27 6 402 489 3051 3245
Fragments arranged by size:
3245 3051 2364 489 402 27 6
BsiEI CG_ry'CG
Cuts at: 104 2535 3859 7126 7172 8380 104
Size: 2431 1324 3267 46 1208 1308
Fragments arranged by size:
3267 2431 1324 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 365 404 853 2129 2171 2822 3446 3581 4640
Size: 39 449 1276 42 651 624 135 1059
Cuts at: 4640 4997 5132 5345 5546 6598 7376 7566 8358
Size: 357 135 213 201 1052 778 190 792
Cuts at: 8358 365
Size: 1591
Fragments arranged by size:
1591 1276 1059 1052 792 778 651 624
449 357 213 201 190 135 135 42
39
BsiWI C'GTAC_G
Cuts at: 638 638
Size: 9584
BsmI GAATG_Cn'
Cuts at: 4428 4428
Size: 9584
BsmBI CGTCTCn'nnnn_
Cuts at: 2926 2950 3721 4191 5888 6100 2926
Size: 24 771 470 1697 212 6410
Fragments arranged by size:
6410 1697 771 470 212 24
Bsp1286I G_dGCh'C
Cuts at: 365 393 404 853 1978 2129 2171 2268 2648
Size: 28 11 449 1125 151 42 97 380
Cuts at: 2648 2822 3221 3395 3446 3581 3630 3835 4330
Size: 174 399 174 51 135 49 205 495
Cuts at: 4330 4439 4640 4997 5006 5132 5246 5345 5450
Size: 109 201 357 9 126 114 99 105
Cuts at: 5450 5546 5914 6082 6170 6214 6360 6369 6485
Size: 96 368 168 88 44 146 9 116
Cuts at: 6485 6598 7209 7302 7376 7566 7628 8358 365
Size: 113 611 93 74 190 62 730 1591
Fragments arranged by size:
1591 1125 730 611 495 449 399 380
368 357 205 201 190 174 174 168
151 146 135 126 116 114 113 109
105 99 97 96 93 88 74 62
51 49 44 42 28 11 9 9
BspEI T'CCGG_A
Cuts at: 2759 3008 3092 4486 8804 2759
Size: 249 84 1394 4318 3539
Fragments arranged by size:
4318 3539 1394 249 84
BspHI T'CATG_A
Cuts at: 5453 8760 5453
Size: 3307 6277
BspMI ACCTGCnnnn'nnnn_
Cuts at: 170 1889 2236 2567 3230 3298 3342 4501 5119
Size: 1719 347 331 663 68 44 1159 618
Cuts at: 5119 6353 6414 6467 6634 6848 7150 7531 170
Size: 1234 61 53 167 214 302 381 2223
Fragments arranged by size:
2223 1719 1234 1159 663 618 381 347
331 302 214 167 68 61 53 44
BsrBI CCG'CTC
Cuts at: 50 830 2940 3855 4020 6141 7876 7930 9414
Size: 780 2110 915 165 2121 1735 54 1484
Cuts at: 9414 9579 50
Size: 165 55
Fragments arranged by size:
2121 2110 1735 1484 915 780 165 165
55 54
BsrDI GCAATG_nn'
Cuts at: 260 7496 260
Size: 7236 2348
BsrFI r'CCGG_y
Cuts at: 989 2734 3170 3894 5971 6053 7582 7763 7972
Size: 1745 436 724 2077 82 1529 181 209
Cuts at: 7972 989
Size: 2601
Fragments arranged by size:
2601 2077 1745 1529 724 436 209 181
82
BsrGI T'GTAC_A
Cuts at: 1562 5291 6437 1562
Size: 3729 1146 4709
Fragments arranged by size:
4709 3729 1146
BssHII G'CGCG_C
Cuts at: 6197 6197
Size: 9584
BssSI C'ACGA_G
Cuts at: 204 1641 1914 3520 4176 7855 8213 204
Size: 1437 273 1606 656 3679 358 1575
Fragments arranged by size:
3679 1606 1575 1437 656 358 273
BstAPI GCAn_nnn'nTGC
Cuts at: 369 2724 2949 3036 4443 5099 5355 5430 6364
Size: 2355 225 87 1407 656 256 75 934
Cuts at: 6364 6961 369
Size: 597 2992
Fragments arranged by size:
2992 2355 1407 934 656 597 256 225
87 75
BstBI TT'CG_AA
Cuts at: 7945 7945
Size: 9584
BstEII G'GTnAC_C
Cuts at: 1482 3651 1482
Size: 2169 7415
BstXI CCAn_nnnn'nTGG
Cuts at: 1275 2868 4035 5253 5736 6508 6574 1275
Size: 1593 1167 1218 483 772 66 4285
Fragments arranged by size:
4285 1593 1218 1167 772 483 66
BstYI r'GATC_y
Cuts at: 1 629 3995 4778 4797 6014 6531 6609 7076
Size: 628 3366 783 19 1217 517 78 467
Cuts at: 7076 7099 7434 7680 8692 8778 8790 9136 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
3366 1217 1012 783 628 517 467 449
346 335 246 86 78 23 19 12
Bsu36I CC'TnA_GG
Cuts at: 407 3410 407
Size: 3003 6581
BtgI C'CryG_G
Cuts at: 532 1268 1547 1865 2144 2189 3503 3779 4034
Size: 736 279 318 279 45 1314 276 255
Cuts at: 4034 4235 4908 5857 6224 6305 532
Size: 201 673 949 367 81 3811
Fragments arranged by size:
3811 1314 949 736 673 367 318 279
279 276 255 201 81 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4483 5993 7534 7715 7878 4483
Size: 1510 1541 181 163 6189
Fragments arranged by size:
6189 1541 1510 181 163
BtsI GCAGTG_nn'
Cuts at: 867 1532 2109 2201 4433 4888 5056 5145 5431
Size: 665 577 92 2232 455 168 89 286
Cuts at: 5431 6827 867
Size: 1396 3624
Fragments arranged by size:
3624 2232 1396 665 577 455 286 168
92 89
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3924 3959 3924
Size: 35 9549
DraI TTT'AAA
Cuts at: 6583 6583
Size: 9584
DraIII CAC_nnn'GTG
Cuts at: 369 4443 5732 6364 369
Size: 4074 1289 632 3589
Fragments arranged by size:
4074 3589 1289 632
DrdI GACnn_nn'nnGTC
Cuts at: 2710 3883 7290 8148 2710
Size: 1173 3407 858 4146
Fragments arranged by size:
4146 3407 1173 858
EaeI y'GGCC_r
Cuts at: 659 761 1325 1550 1931 2074 2147 3843 3856
Size: 102 564 225 381 143 73 1696 13
Cuts at: 3856 4025 4064 4208 4835 4848 5193 5645 6113
Size: 169 39 144 627 13 345 452 468
Cuts at: 6113 6227 6308 6498 7169 7343 7734 7761 9106
Size: 114 81 190 671 174 391 27 1345
Cuts at: 9106 659
Size: 1137
Fragments arranged by size:
1696 1345 1137 671 627 564 468 452
391 381 345 225 190 174 169 144
143 114 102 81 73 39 27 13
13
EagI C'GGCC_G
Cuts at: 3856 7169 3856
Size: 3313 6271
EarI CTCTTCn'nnn_
Cuts at: 1145 1354 1625 1879 2276 3266 4885 6485 7607
Size: 209 271 254 397 990 1619 1600 1122
Cuts at: 7607 7817 1145
Size: 210 2912
Fragments arranged by size:
2912 1619 1600 1122 990 397 271 254
210 209
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 559 2656 3184 3338 4603 6303 7792 8102 8248
Size: 2097 528 154 1265 1700 1489 310 146
Cuts at: 8248 9394 559
Size: 1146 749
Fragments arranged by size:
2097 1700 1489 1265 1146 749 528 310
154 146
EcoICRI GAG'CTC
Cuts at: 2127 4638 5343 6596 2127
Size: 2511 705 1253 5115
Fragments arranged by size:
5115 2511 1253 705
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 760 964 969 1202 1258 1453 1723 1792
Size: 144 204 5 233 56 195 270 69
Cuts at: 1792 2383 2401 2764 2890 2947 3040 3115 3124
Size: 591 18 363 126 57 93 75 9
Cuts at: 3124 3222 3362 3595 3821 4420 4438 5275 5404
Size: 98 140 233 226 599 18 837 129
Cuts at: 5404 5440 5560 5593 5821 5965 5977 6154 6499
Size: 36 120 33 228 144 12 177 345
Cuts at: 6499 7409 7841 8588 8856 616
Size: 910 432 747 268 1344
Fragments arranged by size:
1344 910 837 747 599 591 432 363
345 270 268 233 233 228 226 204
195 177 144 144 140 129 126 120
98 93 75 69 57 56 36 33
18 18 12 9 5
EcoNI CCTnn'n_nnAGG
Cuts at: 433 1522 2827 3141 4210 5163 5596 5611 5826
Size: 1089 1305 314 1069 953 433 15 215
Cuts at: 5826 6133 6490 433
Size: 307 357 3527
Fragments arranged by size:
3527 1305 1089 1069 953 433 357 314
307 215 15
EcoO109I rG'GnC_Cy
Cuts at: 1233 1659 2382 3431 3465 4470 5082 5301 5898
Size: 426 723 1049 34 1005 612 219 597
Cuts at: 5898 6155 6166 6167 6723 1233
Size: 257 11 1 556 4094
Fragments arranged by size:
4094 1049 1005 723 612 597 556 426
257 219 34 11 1
EcoRI G'AATT_C
Cuts at: 6588 6588
Size: 9584
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 630 1263 4944 7603 630
Size: 633 3681 2659 2611
Fragments arranged by size:
3681 2659 2611 633
FspI TGC'GCA
Cuts at: 2755 7365 2755
Size: 4610 4974
FspAI rTGC'GCAy
Cuts at: 2755 2755
Size: 9584
HaeII r_GCGC'y
Cuts at: 510 1158 1571 2594 2780 3103 3911 4034 5356
Size: 648 413 1023 186 323 808 123 1322
Cuts at: 5356 5644 6396 6476 7018 7266 8288 9267 510
Size: 288 752 80 542 248 1022 979 827
Fragments arranged by size:
1322 1023 1022 979 827 808 752 648
542 413 323 288 248 186 123 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1031 1034 1063 1066 1405 1437 2482 2514 3195
Size: 3 29 3 339 32 1045 32 681
Cuts at: 3195 3227 3730 3762 4282 4314 4330 4362 5221
Size: 32 503 32 520 32 16 32 859
Cuts at: 5221 5253 7426 7456 7458 7488 1031
Size: 32 2173 30 2 30 3127
Fragments arranged by size:
3127 2173 1045 859 681 520 503 339
32 32 32 32 32 32 32 30
30 29 16 3 3 2
HincII GTy'rAC
Cuts at: 1491 4338 5622 6623 1491
Size: 2847 1284 1001 4452
Fragments arranged by size:
4452 2847 1284 1001
HindIII A'AGCT_T
Cuts at: 1498 1498
Size: 9584
KasI G'GCGC_C
Cuts at: 506 6392 6472 7014 7262 506
Size: 5886 80 542 248 2828
Fragments arranged by size:
5886 2828 542 248 80
KpnI G_GTAC'C
Cuts at: 4008 6604 4008
Size: 2596 6988
MluI A'CGCG_T
Cuts at: 9017 9017
Size: 9584
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1367 3830 8255 8439 1367
Size: 2463 4425 184 2512
Fragments arranged by size:
4425 2512 2463 184
MscI TGG'CCA
Cuts at: 661 1552 1933 3845 4027 4210 4837 4850 5195
Size: 891 381 1912 182 183 627 13 345
Cuts at: 5195 5647 6500 7345 661
Size: 452 853 845 2900
Fragments arranged by size:
2900 1912 891 853 845 627 452 381
345 183 182 13
MslI CAynn'nnrTG
Cuts at: 112 655 1273 2134 2194 2518 2737 4459 5413
Size: 543 618 861 60 324 219 1722 954
Cuts at: 5413 5734 7573 7700 112
Size: 321 1839 127 1996
Fragments arranged by size:
1996 1839 1722 954 861 618 543 324
321 219 127 60
MspA1I CmG'CkG
Cuts at: 180 480 729 905 2159 2660 2724 3159 3379
Size: 300 249 176 1254 501 64 435 220
Cuts at: 3379 4002 4267 5517 6695 7009 7369 8382 8627
Size: 623 265 1250 1178 314 360 1013 245
Cuts at: 8627 180
Size: 1137
Fragments arranged by size:
1254 1250 1178 1137 1013 623 501 435
360 314 300 265 249 245 220 176
64
NaeI GCC'GGC
Cuts at: 5973 7765 5973
Size: 1792 7792
NarI GG'CG_CC
Cuts at: 507 6393 6473 7015 7263 507
Size: 5886 80 542 248 2828
Fragments arranged by size:
5886 2828 542 248 80
NcoI C'CATG_G
Cuts at: 1268 1547 2189 3779 5857 1268
Size: 279 642 1590 2078 4995
Fragments arranged by size:
4995 2078 1590 642 279
NdeI CA'TA_TG
Cuts at: 473 473
Size: 9584
NgoMIV G'CCGG_C
Cuts at: 5971 7763 5971
Size: 1792 7792
NspI r_CATG'y
Cuts at: 2073 2742 5631 6637 7137 8044 2073
Size: 669 2889 1006 500 907 3613
Fragments arranged by size:
3613 2889 1006 907 669 500
PciI A'CATG_T
Cuts at: 8040 8040
Size: 9584
PflMI CCAn_nnn'nTGG
Cuts at: 729 3780 4101 729
Size: 3051 321 6212
Fragments arranged by size:
6212 3051 321
PfoI T'CCnGG_A
Cuts at: 1661 2048 2792 2867 3677 4988 5264 5537 5930
Size: 387 744 75 810 1311 276 273 393
Cuts at: 5930 1661
Size: 5315
Fragments arranged by size:
5315 1311 810 744 393 387 276 273
75
PmeI GTTT'AAAC
Cuts at: 6583 6583
Size: 9584
PmlI CAC'GTG
Cuts at: 1805 4440 4785 5007 5274 5732 1805
Size: 2635 345 222 267 458 5657
Fragments arranged by size:
5657 2635 458 345 267 222
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6085 6117 8723 8755 6085
Size: 32 2606 32 6914
Fragments arranged by size:
6914 2606 32 32
PpuMI rG'GwC_Cy
Cuts at: 1233 1659 2382 3431 5898 1233
Size: 426 723 1049 2467 4919
Fragments arranged by size:
4919 2467 1049 723 426
PshAI GACnn'nnGTC
Cuts at: 2917 2917
Size: 9584
PsiI TTA'TAA
Cuts at: 1355 1355
Size: 9584
PspOMI G'GGCC_C
Cuts at: 6166 6166
Size: 9584
PspXI vC'TCGA_Gb
Cuts at: 5611 5611
Size: 9584
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2050 2082 2050
Size: 32 9552
PstI C_TGCA'G
Cuts at: 437 2247 2720 2848 2954 2999 3041 3203 3227
Size: 1810 473 128 106 45 42 162 24
Cuts at: 3227 4227 4512 5095 5360 5811 6631 7316 437
Size: 1000 285 583 265 451 820 685 2705
Fragments arranged by size:
2705 1810 1000 820 685 583 473 451
285 265 162 128 106 45 42 24
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9584
PvuII CAG'CTG
Cuts at: 180 480 729 2724 4267 5517 7009 7369 180
Size: 300 249 1995 1543 1250 1492 360 2395
Fragments arranged by size:
2395 1995 1543 1492 1250 360 300 249
RsrII CG'GwC_CG
Cuts at: 7779 7779
Size: 9584
SacI G_AGCT'C
Cuts at: 2129 4640 5345 6598 2129
Size: 2511 705 1253 5115
Fragments arranged by size:
5115 2511 1253 705
SalI G'TCGA_C
Cuts at: 4336 6621 4336
Size: 2285 7299
SapI GCTCTTCn'nnn_
Cuts at: 1145 2276 7607 7817 1145
Size: 1131 5331 210 2912
Fragments arranged by size:
5331 2912 1131 210
SbfI CC_TGCA'GG
Cuts at: 2848 2954 6631 2848
Size: 106 3677 5801
Fragments arranged by size:
5801 3677 106
ScaI AGT'ACT
Cuts at: 1966 9101 1966
Size: 7135 2449
SexAI A'CCwGG_T
Cuts at: 4865 5021 4865
Size: 156 9428
SfcI C'TryA_G
Cuts at: 33 433 1367 1512 2243 2716 2844 2950 2995
Size: 400 934 145 731 473 128 106 45
Cuts at: 2995 3037 3199 3223 4223 4508 5091 5356 5807
Size: 42 162 24 1000 285 583 265 451
Cuts at: 5807 6627 7312 8305 8496 33
Size: 820 685 993 191 1121
Fragments arranged by size:
1121 1000 993 934 820 731 685 583
473 451 400 285 265 191 162 145
128 106 45 42 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 769 2230 4132 769
Size: 1461 1902 6221
Fragments arranged by size:
6221 1902 1461
SfoI GGC'GCC
Cuts at: 508 6394 6474 7016 7264 508
Size: 5886 80 542 248 2828
Fragments arranged by size:
5886 2828 542 248 80
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9584
SmaI CCC'GGG
Cuts at: 4325 5923 6370 6450 6606 4325
Size: 1598 447 80 156 7303
Fragments arranged by size:
7303 1598 447 156 80
SmlI C'TyrA_G
Cuts at: 604 1171 1258 4639 5611 5719 6739 7654 8146
Size: 567 87 3381 972 108 1020 915 492
Cuts at: 8146 8408 604
Size: 262 1780
Fragments arranged by size:
3381 1780 1020 972 915 567 492 262
108 87
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9584
SphI G_CATG'C
Cuts at: 2742 6637 7137 2742
Size: 3895 500 5189
Fragments arranged by size:
5189 3895 500
StuI AGG'CCT
Cuts at: 202 3070 3136 3840 5236 6332 202
Size: 2868 66 704 1396 1096 3454
Fragments arranged by size:
3454 2868 1396 1096 704 66
StyI C'CwwG_G
Cuts at: 224 1268 1547 2189 2828 2915 3779 3952 4211
Size: 1044 279 642 639 87 864 173 259
Cuts at: 4211 4379 4526 5857 6073 6718 7072 224
Size: 168 147 1331 216 645 354 2736
Fragments arranged by size:
2736 1331 1044 864 645 642 639 354
279 259 216 173 168 147 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 324 916 2291 3670 7167 7300 7759 7968 324
Size: 592 1375 1379 3497 133 459 209 1940
Fragments arranged by size:
3497 1940 1379 1375 592 459 209 133
TatI w'GTAC_w
Cuts at: 1562 1964 2783 4757 5291 6437 9099 1562
Size: 402 819 1974 534 1146 2662 2047
Fragments arranged by size:
2662 2047 1974 1146 819 534 402
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3592 3624 5651 5683 9101 9133 3592
Size: 32 2027 32 3418 32 4043
Fragments arranged by size:
4043 3418 2027 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1831 3329 7381 1831
Size: 1498 4052 4034
Fragments arranged by size:
4052 4034 1498
XbaI T'CTAG_A
Cuts at: 6615 6615
Size: 9584
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 273 1275 1335 1659 3870 7079 273
Size: 1002 60 324 2211 3209 2778
Fragments arranged by size:
3209 2778 2211 1002 324 60
XhoI C'TCGA_G
Cuts at: 5611 5611
Size: 9584
XmaI C'CCGG_G
Cuts at: 4323 5921 6368 6448 6604 4323
Size: 1598 447 80 156 7303
Fragments arranged by size:
7303 1598 447 156 80
XmnI GAAnn'nnTTC
Cuts at: 3713 4927 3713
Size: 1214 8370
ZraI GAC'GTC
Cuts at: 246 4185 5715 6110 246
Size: 3939 1530 395 3720
Fragments arranged by size:
3939 3720 1530 395
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AvrII BaeI BfrBI BmtI BstZ17I ClaI
EcoRV FseI HpaI MfeI NheI NotI NruI NsiI
PacI PasI SacII SanDI SgrAI SnaBI SrfI SspI
SwaI