Restriction map for pF1KB0016
 (Circular) (Six-base) MAPSORT of: pF1KB0016.seq  Check: 3011  from: 1  to: 8183



pF1KB0016  8183 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:22  ..

AatII G_ACGT'C

Cuts at:   1896    1896 
   Size:       8183
 
AccI GT'mk_AC

Cuts at:   1189    4976    5221    1189 
   Size:       3787     245    4151

  Fragments arranged by size: 

                4151    3787     245
 
Acc65I G'GTAC_C

Cuts at:   5199    5199 
   Size:       8183
 
AclI AA'CG_TT

Cuts at:   8023    8023 
   Size:       8183
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    442     808     835    1180    1871    2057    2809    3790    3851 
   Size:        366      27     345     691     186     752     981      61

Cuts at:   3851    4556    4595    6008    6440    7187    7455     442 
   Size:        705      39    1413     432     747     268    1170

  Fragments arranged by size: 

                1413    1170     981     752     747     705     691     432
                 366     345     268     186      61      39      27
 
AflII C'TTAA_G

Cuts at:   3292    3292 
   Size:       8183
 
AflIII A'CryG_T

Cuts at:    765    2152    2173    4274    5535    6639    7616     765 
   Size:       1387      21    2101    1261    1104     977    1332

  Fragments arranged by size: 

                2101    1387    1332    1261    1104     977      21
 
AgeI A'CCGG_T

Cuts at:   6571    6571 
   Size:       8183
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   4965    4965 
   Size:       8183
 
AlwNI CAG_nnn'CTG

Cuts at:    336     787    2962    3327    4141    5488    7055     336 
   Size:        451    2175     365     814    1347    1567    1464

  Fragments arranged by size: 

                2175    1567    1464    1347     814     451     365
 
ApaI G_GGCC'C

Cuts at:    845     845 
   Size:       8183
 
ApaLI G'TGCA_C

Cuts at:   6953    6953 
   Size:       8183
 
ApoI r'AATT_y

Cuts at:    257     394     599    1285    1437    3300    3592    3733    5187 
   Size:        137     205     686     152    1863     292     141    1454

Cuts at:   5187    7536    7792     257 
   Size:       2349     256     648

  Fragments arranged by size: 

                2349    1863    1454     686     648     292     256     205
                 152     141     137
 
AseI AT'TA_AT

Cuts at:     20    1484      20 
   Size:       1464    6719
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       8183
 
AvaI C'yCGr_G

Cuts at:   1151    1675    3110    3971    5203    1151 
   Size:        524    1435     861    1232    4131

  Fragments arranged by size: 

                4131    1435    1232     861     524
 
BamHI G'GATC_C

Cuts at:   3706    5208    3706 
   Size:       1502    6681
 
BanI G'GyrC_C

Cuts at:   1925    2904    4383    5199    5613    5861    5896    1925 
   Size:        979    1479     816     414     248      35    4212

  Fragments arranged by size: 

                4212    1479     979     816     414     248      35
 
BanII G_rGCy'C

Cuts at:    845    2542    2752    2951    3334    3644    4378    5197    6227 
   Size:       1697     210     199     383     310     734     819    1030

Cuts at:   6227     845 
   Size:       2801

  Fragments arranged by size: 

                2801    1697    1030     819     734     383     310     210
                 199
 
BbeI G_GCGC'C

Cuts at:   1929    5617    5865    1929 
   Size:       3688     248    4247

  Fragments arranged by size: 

                4247    3688     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    778     906    2282    2605    4154    4544     778 
   Size:        128    1376     323    1549     390    4417

  Fragments arranged by size: 

                4417    1549    1376     390     323     128
 
BbvCI CC'TCA_GC

Cuts at:   2417    2417 
   Size:       8183
 
BciVI GTATCCnnnnn_n'

Cuts at:   3810    6081    6842    8183    3810 
   Size:       2271     761    1341    3810

  Fragments arranged by size: 

                3810    2271    1341     761
 
BclI T'GATC_A

Cuts at:   5703    5703 
   Size:       8183
 
BfrBI ATG'CAT

Cuts at:   3531    3531 
   Size:       8183
 
BglII A'GATC_T

Cuts at:    666    4464    5698    7735     666 
   Size:       3798    1234    2037    1114

  Fragments arranged by size: 

                3798    2037    1234    1114
 
BlpI GC'TnA_GC

Cuts at:    205    2501    3328    5295     205 
   Size:       2296     827    1967    3093

  Fragments arranged by size: 

                3093    2296    1967     827
 
Bme1580I G_kGCm'C

Cuts at:    166     845    1100    2230    2316    4388    5808    5901    6957 
   Size:        679     255    1130      86    2072    1420      93    1056

Cuts at:   6957     166 
   Size:       1392

  Fragments arranged by size: 

                2072    1420    1392    1130    1056     679     255      93
                  86
 
BmgBI CAC'GTC

Cuts at:    766     766 
   Size:       8183
 
BmrI ACTGGGnnnn_n'

Cuts at:    578    1605    3287    4989    5395    5476    5811    7955     578 
   Size:       1027    1682    1702     406      81     335    2144     806

  Fragments arranged by size: 

                2144    1702    1682    1027     806     406     335      81
            
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4352    6550    4352 
   Size:       2198    5985
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    809    1131    1590    1669    2713    3448    3466    4388    4432 
   Size:        322     459      79    1044     735      18     922      44

Cuts at:   4432    4445    5024     809 
   Size:         13     579    3968

  Fragments arranged by size: 

                3968    1044     922     735     579     459     322      79
                  44      18      13
 
Bpu10I CC'TnA_GC

Cuts at:   2189    2417    2687    2984    4430    2189 
   Size:        228     270     297    1446    5942

  Fragments arranged by size: 

                5942    1446     297     270     228
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    367    3196    4322    5359    6238    6730    7028     367 
   Size:       2829    1126    1037     879     492     298    1522

  Fragments arranged by size: 

                2829    1522    1126    1037     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    578    2476    3764    5164    7887     578 
   Size:       1898    1288    1400    2723     874

  Fragments arranged by size: 

                2723    1898    1400    1288     874
 
BsaAI yAC'GTr

Cuts at:   5536    5536 
   Size:       8183
 
BsaBI GATnn'nnATC

Cuts at:      6    4246    5697    7734       6 
   Size:       4240    1451    2037     455

  Fragments arranged by size: 

                4240    2037    1451     455
 
BsaHI Gr'CG_yC

Cuts at:   1893    1917    1926    3198    5614    5862    6564    8060    1893 
   Size:         24       9    1272    2416     248     702    1496    2016

  Fragments arranged by size: 

                2416    2016    1496    1272     702     248      24       9
            
 
BsaWI w'CCGG_w

Cuts at:   1374    5372    5893    6571    6845    6992    7403    1374 
   Size:       3998     521     678     274     147     411    2154

  Fragments arranged by size: 

                3998    2154     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   2933    2963    2933 
   Size:         30    8153
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    202    1338    1666    4091    4273    4924     202 
   Size:       1136     328    2425     182     651    3461

  Fragments arranged by size: 

                3461    2425    1136     651     328     182
 
BseYI C'CCAG_C

Cuts at:    838    1691    5608    6366    6943    7439     838 
   Size:        853    3917     758     577     496    1582

  Fragments arranged by size: 

                3917    1582     853     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    670    4019    4319    4751     670 
   Size:       3349     300     432    4102

  Fragments arranged by size: 

                4102    3349     432     300
 
BsiEI CG_ry'CG

Cuts at:    104     219     265    5725    5771    6979     104 
   Size:        115      46    5460      46    1208    1308

  Fragments arranged by size: 

                5460    1308    1208     115      46      46
 
BsiHKAI G_wGCw'C

Cuts at:   2688    2951    3644    4846    5197    5975    6165    6957    2688 
   Size:        263     693    1202     351     778     190     792    3914

  Fragments arranged by size: 

                3914    1202     792     778     693     351     263     190
            
 
BsiWI C'GTAC_G

Cuts at:   3819    3819 
   Size:       8183
 
BsmI GAATG_Cn'

Cuts at:   1987    3531    4932    1987 
   Size:       1544    1401    5238

  Fragments arranged by size: 

                5238    1544    1401
 
Bsp1286I G_dGCh'C

Cuts at:    166     845    1100    2230    2316    2542    2688    2752    2951 
   Size:        679     255    1130      86     226     146      64     199

Cuts at:   2951    3334    3644    4378    4388    4846    5197    5808    5901 
   Size:        383     310     734      10     458     351     611      93

Cuts at:   5901    5975    6165    6227    6957     166 
   Size:         74     190      62     730    1392

  Fragments arranged by size: 

                1392    1130     734     730     679     611     458     383
                 351     310     255     226     199     190     146      93
                  86      74      64      62      10
 
BspEI T'CCGG_A

Cuts at:   7403    7403 
   Size:       8183
 
BspHI T'CATG_A

Cuts at:   1724    2030    2676    2870    7359    1724 
   Size:        306     646     194    4489    2548

  Fragments arranged by size: 

                4489    2548     646     306     194
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   5233    5447    5749    6130    5233 
   Size:        214     302     381    7286

  Fragments arranged by size: 

                7286     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     204    2127    2193    6475    6529    8013    8178      50 
   Size:        154    1923      66    4282      54    1484     165      55

  Fragments arranged by size: 

                4282    1923    1484     165     154      66      55      54
            
 
BsrDI GCAATG_nn'

Cuts at:    359     869    2936    6095     359 
   Size:        510    2067    3159    2447

  Fragments arranged by size: 

                3159    2447    2067     510
 
BsrFI r'CCGG_y

Cuts at:    244    6181    6362    6571     244 
   Size:       5937     181     209    1856

  Fragments arranged by size: 

                5937    1856     209     181
 
BsrGI T'GTAC_A

Cuts at:   1763    2067    1763 
   Size:        304    7879
 
BssSI C'ACGA_G

Cuts at:   1808    1945    6454    6812    1808 
   Size:        137    4509     358    3179

  Fragments arranged by size: 

                4509    3179     358     137
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1755    2359    4306    5560    1755 
   Size:        604    1947    1254    4378

  Fragments arranged by size: 

                4378    1947    1254     604
 
BstBI TT'CG_AA

Cuts at:   2451    6544    2451 
   Size:       4093    4090
 
BstEII G'GTnAC_C

Cuts at:    504     891    4266     504 
   Size:        387    3375    4421

  Fragments arranged by size: 

                4421    3375     387
 
BstXI CCAn_nnnn'nTGG

Cuts at:    852    1613    3486     852 
   Size:        761    1873    5549

  Fragments arranged by size: 

                5549    1873     761
 
BstYI r'GATC_y

Cuts at:      1     198     412     425     453     666    1974    2844    3417 
   Size:        197     214      13      28     213    1308     870     573

Cuts at:   3417    3706    4464    5160    5208    5675    5698    6033    6279 
   Size:        289     758     696      48     467      23     335     246

Cuts at:   6279    7291    7377    7389    7735       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1308    1012     870     758     696     573     467     449
                 346     335     289     246     214     213     197      86
                  48      28      23      13      12
 
BstZ17I GTA'TAC

Cuts at:   1190    1190 
   Size:       8183
 
Bsu36I CC'TnA_GG

Cuts at:    689    1921     689 
   Size:       1232    6951
 
BtgI C'CryG_G

Cuts at:    107    1513    2006    3479     107 
   Size:       1406     493    1473    4811

  Fragments arranged by size: 

                4811    1473    1406     493
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   6133    6314    6477    6133 
   Size:        181     163    7839

  Fragments arranged by size: 

                7839     181     163
 
BtsI GCAGTG_nn'

Cuts at:   2137    3223    3853    4092    5426    2137 
   Size:       1086     630     239    1334    4894

  Fragments arranged by size: 

                4894    1334    1086     630     239
 
DraI TTT'AAA

Cuts at:   5182    5182 
   Size:       8183
 
DraIII CAC_nnn'GTG

Cuts at:   3018    3018 
   Size:       8183
 
DrdI GACnn_nn'nnGTC

Cuts at:   2332    3786    5889    6747    2332 
   Size:       1454    2103     858    3768

  Fragments arranged by size: 

                3768    2103    1454     858
 
EaeI y'GGCC_r

Cuts at:    216     262    3926    4760    5768    5942    6333    6360    7705 
   Size:         46    3664     834    1008     174     391      27    1345

Cuts at:   7705     216 
   Size:        694

  Fragments arranged by size: 

                3664    1345    1008     834     694     391     174      46
                  27
 
EagI C'GGCC_G

Cuts at:    216     262    5768     216 
   Size:         46    5506    2631

  Fragments arranged by size: 

                5506    2631      46
 
EarI CTCTTCn'nnn_

Cuts at:    748    1406    1545    2663    3683    4232    4366    4571    4614 
   Size:        658     139    1118    1020     549     134     205      43

Cuts at:   4614    4778    6206    6416     748 
   Size:        164    1428     210    2515

  Fragments arranged by size: 

                2515    1428    1118    1020     658     549     210     205
                 164     139     134      43
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    154     250    1404    3297    4521    6391    6701    6847    7993 
   Size:         96    1154    1893    1224    1870     310     146    1146

Cuts at:   7993     154 
   Size:        344

  Fragments arranged by size: 

                1893    1870    1224    1154    1146     344     310     146
                  96
 
EcoICRI GAG'CTC

Cuts at:   2949    3642    5195    2949 
   Size:        693    1553    5937

  Fragments arranged by size: 

                5937    1553     693
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    442     808     809     835    1131    1180    1590    1669    1871 
   Size:        366       1      26     296      49     410      79     202

Cuts at:   1871    2057    2713    2809    3448    3466    3790    3851    4388 
   Size:        186     656      96     639      18     324      61     537

Cuts at:   4388    4432    4445    4556    4595    5024    6008    6440    7187 
   Size:         44      13     111      39     429     984     432     747

Cuts at:   7187    7455     442 
   Size:        268    1170

  Fragments arranged by size: 

                1170     984     747     656     639     537     432     429
                 410     366     324     296     268     202     186     111
                  96      79      61      49      44      39      26      18
                  13       1
 
EcoO109I rG'GnC_Cy

Cuts at:   3840    4958    5322    3840 
   Size:       1118     364    6701

  Fragments arranged by size: 

                6701    1118     364
 
EcoRI G'AATT_C

Cuts at:    599    3300    5187     599 
   Size:       2701    1887    3595

  Fragments arranged by size: 

                3595    2701    1887
 
EcoRV GAT'ATC

Cuts at:    550    1344    2260     550 
   Size:        794     916    6473

  Fragments arranged by size: 

                6473     916     794
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   4846    6202    4846 
   Size:       1356    6827
 
FspI TGC'GCA

Cuts at:   4938    5964    4938 
   Size:       1026    7157
 
HaeII r_GCGC'y

Cuts at:    336    1929    5617    5865    6887    7866     336 
   Size:       1593    3688     248    1022     979     653

  Fragments arranged by size: 

                3688    1593    1022     979     653     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1328    1333    1360    1365    1791    1823    2734    2766    3169 
   Size:          5      27       5     426      32     911      32     403

Cuts at:   3169    3201    3898    3930    4867    4899    6025    6055    6057 
   Size:         32     697      32     937      32    1126      30       2

Cuts at:   6057    6087    1328 
   Size:         30    3424

  Fragments arranged by size: 

                3424    1126     937     911     697     426     403      32
                  32      32      32      32      30      30      27       5
                   5       2
 
HincII GTy'rAC

Cuts at:    972    5222     972 
   Size:       4250    3933
 
HindIII A'AGCT_T

Cuts at:    306     306 
   Size:       8183
 
HpaI GTT'AAC

Cuts at:    972     972 
   Size:       8183
 
KasI G'GCGC_C

Cuts at:   1925    5613    5861    1925 
   Size:       3688     248    4247

  Fragments arranged by size: 

                4247    3688     248
 
KpnI G_GTAC'C

Cuts at:   5203    5203 
   Size:       8183
 
MluI A'CGCG_T

Cuts at:   7616    7616 
   Size:       8183
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1414    2239    2622    2798    4440    6854    7038    1414 
   Size:        825     383     176    1642    2414     184    2559

  Fragments arranged by size: 

                2559    2414    1642     825     383     184     176
 
MscI TGG'CCA

Cuts at:   3928    4762    5944    3928 
   Size:        834    1182    6167

  Fragments arranged by size: 

                6167    1182     834
 
MslI CAynn'nnrTG

Cuts at:    736     940    1484    2681    3261    3484    6172    6299     736 
   Size:        204     544    1197     580     223    2688     127    2620

  Fragments arranged by size: 

                2688    2620    1197     580     544     223     204     127
            
 
MspA1I CmG'CkG

Cuts at:   2505    3668    3713    4018    5294    5608    5968    6981    7226 
   Size:       1163      45     305    1276     314     360    1013     245

Cuts at:   7226    2505 
   Size:       3462

  Fragments arranged by size: 

                3462    1276    1163    1013     360     314     305     245
                  45
 
NaeI GCC'GGC

Cuts at:   6364    6364 
   Size:       8183
 
NarI GG'CG_CC

Cuts at:   1926    5614    5862    1926 
   Size:       3688     248    4247

  Fragments arranged by size: 

                4247    3688     248
 
NcoI C'CATG_G

Cuts at:    107    2006    3479     107 
   Size:       1899    1473    4811

  Fragments arranged by size: 

                4811    1899    1473
 
NdeI CA'TA_TG

Cuts at:    612    1256    4164    4501     612 
   Size:        644    2908     337    4294

  Fragments arranged by size: 

                4294    2908     644     337
 
NgoMIV G'CCGG_C

Cuts at:   6362    6362 
   Size:       8183
 
NsiI A_TGCA'T

Cuts at:   3533    3533 
   Size:       8183
 
NspI r_CATG'y

Cuts at:    939    1452    2144    2156    2177    4278    4309    5236    5736 
   Size:        513     692      12      21    2101      31     927     500

Cuts at:   5736    6643     939 
   Size:        907    2479

  Fragments arranged by size: 

                2479    2101     927     907     692     513     500      31
                  21      12
 
PciI A'CATG_T

Cuts at:   2152    2173    4274    6639    2152 
   Size:         21    2101    2365    3696

  Fragments arranged by size: 

                3696    2365    2101      21
 
PflMI CCAn_nnn'nTGG

Cuts at:    275    2548    2919    3954     275 
   Size:       2273     371    1035    4504

  Fragments arranged by size: 

                4504    2273    1035     371
 
PfoI T'CCnGG_A

Cuts at:    449    1457    4469     449 
   Size:       1008    3012    4163

  Fragments arranged by size: 

                4163    3012    1008
 
PmeI GTTT'AAAC

Cuts at:   5182    5182 
   Size:       8183
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    558     590    7322    7354     558 
   Size:         32    6732      32    1387

  Fragments arranged by size: 

                6732    1387      32      32
 
PspOMI G'GGCC_C

Cuts at:    841     841 
   Size:       8183
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   1163    1195    1163 
   Size:         32    8151
 
PstI C_TGCA'G

Cuts at:   1641    3848    4818    5230    5915    1641 
   Size:       2207     970     412     685    3909

  Fragments arranged by size: 

                3909    2207     970     685     412
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       8183
 
PvuII CAG'CTG

Cuts at:   2505    3668    3713    4018    5608    5968    2505 
   Size:       1163      45     305    1590     360    4720

  Fragments arranged by size: 

                4720    1590    1163     360     305      45
 
RsrII CG'GwC_CG

Cuts at:   3338    6378    3338 
   Size:       3040    5143
 
SacI G_AGCT'C

Cuts at:   2951    3644    5197    2951 
   Size:        693    1553    5937

  Fragments arranged by size: 

                5937    1553     693
 
SalI G'TCGA_C

Cuts at:   5220    5220 
   Size:       8183
 
SapI GCTCTTCn'nnn_

Cuts at:   4366    6206    6416    4366 
   Size:       1840     210    6133

  Fragments arranged by size: 

                6133    1840     210
 
SbfI CC_TGCA'GG

Cuts at:   5230    5230 
   Size:       8183
 
ScaI AGT'ACT

Cuts at:   7700    7700 
   Size:       8183
 
SfcI C'TryA_G

Cuts at:     33    1637    2941    2995    3631    3844    4814    5226    5911 
   Size:       1604    1304      54     636     213     970     412     685

Cuts at:   5911    6904    7095      33 
   Size:        993     191    1121

  Fragments arranged by size: 

                1604    1304    1121     993     970     685     636     412
                 213     191      54
 
SfoI GGC'GCC

Cuts at:   1927    5615    5863    1927 
   Size:       3688     248    4247

  Fragments arranged by size: 

                4247    3688     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       8183
 
SmaI CCC'GGG

Cuts at:   5205    5205 
   Size:       8183
 
SmlI C'TyrA_G

Cuts at:    346    1151    3110    3175    3292    3971    4337    5338    6253 
   Size:        805    1959      65     117     679     366    1001     915

Cuts at:   6253    6745    7007     346 
   Size:        492     262    1522

  Fragments arranged by size: 

                1959    1522    1001     915     805     679     492     366
                 262     117      65
 
SpeI A'CTAG_T

Cuts at:     67    1272      67 
   Size:       1205    6978
 
SphI G_CATG'C

Cuts at:   2144    4309    5236    5736    2144 
   Size:       2165     927     500    4591

  Fragments arranged by size: 

                4591    2165     927     500
 
StuI AGG'CCT

Cuts at:   2590    2590 
   Size:       8183
 
StyI C'CwwG_G

Cuts at:    107     269     845    2006    3479    4354    4679    5317    5671 
   Size:        162     576    1161    1473     875     325     638     354

Cuts at:   5671     107 
   Size:       2619

  Fragments arranged by size: 

                2619    1473    1161     875     638     576     354     325
                 162
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    634    2065    3021    3355    4541    5020    5766    5899    6358 
   Size:       1431     956     334    1186     479     746     133     459

Cuts at:   6358    6567     634 
   Size:        209    2250

  Fragments arranged by size: 

                2250    1431    1186     956     746     479     459     334
                 209     133
 
TatI w'GTAC_w

Cuts at:   1763    1867    2067    7698    1763 
   Size:        104     200    5631    2248

  Fragments arranged by size: 

                5631    2248     200     104
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1361    1393    7700    7732    1361 
   Size:         32    6307      32    1812

  Fragments arranged by size: 

                6307    1812      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    777    5980     777 
   Size:       5203    2980
 
XbaI T'CTAG_A

Cuts at:   5214    5214 
   Size:       8183
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    114     366    1740    2571    3564    5678     114 
   Size:        252    1374     831     993    2114    2619

  Fragments arranged by size: 

                2619    2114    1374     993     831     252
 
XhoI C'TCGA_G

Cuts at:   1151    3110    3971    1151 
   Size:       1959     861    5363

  Fragments arranged by size: 

                5363    1959     861
 
XmaI C'CCGG_G

Cuts at:   5203    5203 
   Size:       8183
 
XmnI GAAnn'nnTTC

Cuts at:    599    2111    2672    3137    4151    4241     599 
   Size:       1512     561     465    1014      90    4541

  Fragments arranged by size: 

                4541    1512    1014     561     465      90
 
ZraI GAC'GTC

Cuts at:   1894    1894 
   Size:       8183
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI
    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NsiI     NspI     PciI    PflMI     PfoI     PmeI     PpiI
   PspOMI     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AhdI     AleI     AloI     AscI    AvrII     BaeI
     BcgI     BglI     BmtI    BsmBI   BssHII     ClaI    CspCI    EcoNI
     FseI    FspAI     MfeI     NheI     NotI     NruI     PacI     PasI
     PmlI    PpuMI    PshAI     PsiI    PspXI    SacII    SanDI    SexAI
     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI
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