(Circular) (Six-base) MAPSORT of: pF1KB0016.seq Check: 3011 from: 1 to: 8183 pF1KB0016 8183 bp With 182 enzymes: SgfI * June 20, 2008 10:22 .. AatII G_ACGT'C Cuts at: 1896 1896 Size: 8183 AccI GT'mk_AC Cuts at: 1189 4976 5221 1189 Size: 3787 245 4151 Fragments arranged by size: 4151 3787 245 Acc65I G'GTAC_C Cuts at: 5199 5199 Size: 8183 AclI AA'CG_TT Cuts at: 8023 8023 Size: 8183 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 442 808 835 1180 1871 2057 2809 3790 3851 Size: 366 27 345 691 186 752 981 61 Cuts at: 3851 4556 4595 6008 6440 7187 7455 442 Size: 705 39 1413 432 747 268 1170 Fragments arranged by size: 1413 1170 981 752 747 705 691 432 366 345 268 186 61 39 27 AflII C'TTAA_G Cuts at: 3292 3292 Size: 8183 AflIII A'CryG_T Cuts at: 765 2152 2173 4274 5535 6639 7616 765 Size: 1387 21 2101 1261 1104 977 1332 Fragments arranged by size: 2101 1387 1332 1261 1104 977 21 AgeI A'CCGG_T Cuts at: 6571 6571 Size: 8183 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 4965 4965 Size: 8183 AlwNI CAG_nnn'CTG Cuts at: 336 787 2962 3327 4141 5488 7055 336 Size: 451 2175 365 814 1347 1567 1464 Fragments arranged by size: 2175 1567 1464 1347 814 451 365 ApaI G_GGCC'C Cuts at: 845 845 Size: 8183 ApaLI G'TGCA_C Cuts at: 6953 6953 Size: 8183 ApoI r'AATT_y Cuts at: 257 394 599 1285 1437 3300 3592 3733 5187 Size: 137 205 686 152 1863 292 141 1454 Cuts at: 5187 7536 7792 257 Size: 2349 256 648 Fragments arranged by size: 2349 1863 1454 686 648 292 256 205 152 141 137 AseI AT'TA_AT Cuts at: 20 1484 20 Size: 1464 6719 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 8183 AvaI C'yCGr_G Cuts at: 1151 1675 3110 3971 5203 1151 Size: 524 1435 861 1232 4131 Fragments arranged by size: 4131 1435 1232 861 524 BamHI G'GATC_C Cuts at: 3706 5208 3706 Size: 1502 6681 BanI G'GyrC_C Cuts at: 1925 2904 4383 5199 5613 5861 5896 1925 Size: 979 1479 816 414 248 35 4212 Fragments arranged by size: 4212 1479 979 816 414 248 35 BanII G_rGCy'C Cuts at: 845 2542 2752 2951 3334 3644 4378 5197 6227 Size: 1697 210 199 383 310 734 819 1030 Cuts at: 6227 845 Size: 2801 Fragments arranged by size: 2801 1697 1030 819 734 383 310 210 199 BbeI G_GCGC'C Cuts at: 1929 5617 5865 1929 Size: 3688 248 4247 Fragments arranged by size: 4247 3688 248 BbsI GAAGACnn'nnnn_ Cuts at: 778 906 2282 2605 4154 4544 778 Size: 128 1376 323 1549 390 4417 Fragments arranged by size: 4417 1549 1376 390 323 128 BbvCI CC'TCA_GC Cuts at: 2417 2417 Size: 8183 BciVI GTATCCnnnnn_n' Cuts at: 3810 6081 6842 8183 3810 Size: 2271 761 1341 3810 Fragments arranged by size: 3810 2271 1341 761 BclI T'GATC_A Cuts at: 5703 5703 Size: 8183 BfrBI ATG'CAT Cuts at: 3531 3531 Size: 8183 BglII A'GATC_T Cuts at: 666 4464 5698 7735 666 Size: 3798 1234 2037 1114 Fragments arranged by size: 3798 2037 1234 1114 BlpI GC'TnA_GC Cuts at: 205 2501 3328 5295 205 Size: 2296 827 1967 3093 Fragments arranged by size: 3093 2296 1967 827 Bme1580I G_kGCm'C Cuts at: 166 845 1100 2230 2316 4388 5808 5901 6957 Size: 679 255 1130 86 2072 1420 93 1056 Cuts at: 6957 166 Size: 1392 Fragments arranged by size: 2072 1420 1392 1130 1056 679 255 93 86 BmgBI CAC'GTC Cuts at: 766 766 Size: 8183 BmrI ACTGGGnnnn_n' Cuts at: 578 1605 3287 4989 5395 5476 5811 7955 578 Size: 1027 1682 1702 406 81 335 2144 806 Fragments arranged by size: 2144 1702 1682 1027 806 406 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4352 6550 4352 Size: 2198 5985 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 809 1131 1590 1669 2713 3448 3466 4388 4432 Size: 322 459 79 1044 735 18 922 44 Cuts at: 4432 4445 5024 809 Size: 13 579 3968 Fragments arranged by size: 3968 1044 922 735 579 459 322 79 44 18 13 Bpu10I CC'TnA_GC Cuts at: 2189 2417 2687 2984 4430 2189 Size: 228 270 297 1446 5942 Fragments arranged by size: 5942 1446 297 270 228 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 367 3196 4322 5359 6238 6730 7028 367 Size: 2829 1126 1037 879 492 298 1522 Fragments arranged by size: 2829 1522 1126 1037 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 578 2476 3764 5164 7887 578 Size: 1898 1288 1400 2723 874 Fragments arranged by size: 2723 1898 1400 1288 874 BsaAI yAC'GTr Cuts at: 5536 5536 Size: 8183 BsaBI GATnn'nnATC Cuts at: 6 4246 5697 7734 6 Size: 4240 1451 2037 455 Fragments arranged by size: 4240 2037 1451 455 BsaHI Gr'CG_yC Cuts at: 1893 1917 1926 3198 5614 5862 6564 8060 1893 Size: 24 9 1272 2416 248 702 1496 2016 Fragments arranged by size: 2416 2016 1496 1272 702 248 24 9 BsaWI w'CCGG_w Cuts at: 1374 5372 5893 6571 6845 6992 7403 1374 Size: 3998 521 678 274 147 411 2154 Fragments arranged by size: 3998 2154 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 2933 2963 2933 Size: 30 8153 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 202 1338 1666 4091 4273 4924 202 Size: 1136 328 2425 182 651 3461 Fragments arranged by size: 3461 2425 1136 651 328 182 BseYI C'CCAG_C Cuts at: 838 1691 5608 6366 6943 7439 838 Size: 853 3917 758 577 496 1582 Fragments arranged by size: 3917 1582 853 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 670 4019 4319 4751 670 Size: 3349 300 432 4102 Fragments arranged by size: 4102 3349 432 300 BsiEI CG_ry'CG Cuts at: 104 219 265 5725 5771 6979 104 Size: 115 46 5460 46 1208 1308 Fragments arranged by size: 5460 1308 1208 115 46 46 BsiHKAI G_wGCw'C Cuts at: 2688 2951 3644 4846 5197 5975 6165 6957 2688 Size: 263 693 1202 351 778 190 792 3914 Fragments arranged by size: 3914 1202 792 778 693 351 263 190 BsiWI C'GTAC_G Cuts at: 3819 3819 Size: 8183 BsmI GAATG_Cn' Cuts at: 1987 3531 4932 1987 Size: 1544 1401 5238 Fragments arranged by size: 5238 1544 1401 Bsp1286I G_dGCh'C Cuts at: 166 845 1100 2230 2316 2542 2688 2752 2951 Size: 679 255 1130 86 226 146 64 199 Cuts at: 2951 3334 3644 4378 4388 4846 5197 5808 5901 Size: 383 310 734 10 458 351 611 93 Cuts at: 5901 5975 6165 6227 6957 166 Size: 74 190 62 730 1392 Fragments arranged by size: 1392 1130 734 730 679 611 458 383 351 310 255 226 199 190 146 93 86 74 64 62 10 BspEI T'CCGG_A Cuts at: 7403 7403 Size: 8183 BspHI T'CATG_A Cuts at: 1724 2030 2676 2870 7359 1724 Size: 306 646 194 4489 2548 Fragments arranged by size: 4489 2548 646 306 194 BspMI ACCTGCnnnn'nnnn_ Cuts at: 5233 5447 5749 6130 5233 Size: 214 302 381 7286 Fragments arranged by size: 7286 381 302 214 BsrBI CCG'CTC Cuts at: 50 204 2127 2193 6475 6529 8013 8178 50 Size: 154 1923 66 4282 54 1484 165 55 Fragments arranged by size: 4282 1923 1484 165 154 66 55 54 BsrDI GCAATG_nn' Cuts at: 359 869 2936 6095 359 Size: 510 2067 3159 2447 Fragments arranged by size: 3159 2447 2067 510 BsrFI r'CCGG_y Cuts at: 244 6181 6362 6571 244 Size: 5937 181 209 1856 Fragments arranged by size: 5937 1856 209 181 BsrGI T'GTAC_A Cuts at: 1763 2067 1763 Size: 304 7879 BssSI C'ACGA_G Cuts at: 1808 1945 6454 6812 1808 Size: 137 4509 358 3179 Fragments arranged by size: 4509 3179 358 137 BstAPI GCAn_nnn'nTGC Cuts at: 1755 2359 4306 5560 1755 Size: 604 1947 1254 4378 Fragments arranged by size: 4378 1947 1254 604 BstBI TT'CG_AA Cuts at: 2451 6544 2451 Size: 4093 4090 BstEII G'GTnAC_C Cuts at: 504 891 4266 504 Size: 387 3375 4421 Fragments arranged by size: 4421 3375 387 BstXI CCAn_nnnn'nTGG Cuts at: 852 1613 3486 852 Size: 761 1873 5549 Fragments arranged by size: 5549 1873 761 BstYI r'GATC_y Cuts at: 1 198 412 425 453 666 1974 2844 3417 Size: 197 214 13 28 213 1308 870 573 Cuts at: 3417 3706 4464 5160 5208 5675 5698 6033 6279 Size: 289 758 696 48 467 23 335 246 Cuts at: 6279 7291 7377 7389 7735 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1308 1012 870 758 696 573 467 449 346 335 289 246 214 213 197 86 48 28 23 13 12 BstZ17I GTA'TAC Cuts at: 1190 1190 Size: 8183 Bsu36I CC'TnA_GG Cuts at: 689 1921 689 Size: 1232 6951 BtgI C'CryG_G Cuts at: 107 1513 2006 3479 107 Size: 1406 493 1473 4811 Fragments arranged by size: 4811 1473 1406 493 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 6133 6314 6477 6133 Size: 181 163 7839 Fragments arranged by size: 7839 181 163 BtsI GCAGTG_nn' Cuts at: 2137 3223 3853 4092 5426 2137 Size: 1086 630 239 1334 4894 Fragments arranged by size: 4894 1334 1086 630 239 DraI TTT'AAA Cuts at: 5182 5182 Size: 8183 DraIII CAC_nnn'GTG Cuts at: 3018 3018 Size: 8183 DrdI GACnn_nn'nnGTC Cuts at: 2332 3786 5889 6747 2332 Size: 1454 2103 858 3768 Fragments arranged by size: 3768 2103 1454 858 EaeI y'GGCC_r Cuts at: 216 262 3926 4760 5768 5942 6333 6360 7705 Size: 46 3664 834 1008 174 391 27 1345 Cuts at: 7705 216 Size: 694 Fragments arranged by size: 3664 1345 1008 834 694 391 174 46 27 EagI C'GGCC_G Cuts at: 216 262 5768 216 Size: 46 5506 2631 Fragments arranged by size: 5506 2631 46 EarI CTCTTCn'nnn_ Cuts at: 748 1406 1545 2663 3683 4232 4366 4571 4614 Size: 658 139 1118 1020 549 134 205 43 Cuts at: 4614 4778 6206 6416 748 Size: 164 1428 210 2515 Fragments arranged by size: 2515 1428 1118 1020 658 549 210 205 164 139 134 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 154 250 1404 3297 4521 6391 6701 6847 7993 Size: 96 1154 1893 1224 1870 310 146 1146 Cuts at: 7993 154 Size: 344 Fragments arranged by size: 1893 1870 1224 1154 1146 344 310 146 96 EcoICRI GAG'CTC Cuts at: 2949 3642 5195 2949 Size: 693 1553 5937 Fragments arranged by size: 5937 1553 693 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 442 808 809 835 1131 1180 1590 1669 1871 Size: 366 1 26 296 49 410 79 202 Cuts at: 1871 2057 2713 2809 3448 3466 3790 3851 4388 Size: 186 656 96 639 18 324 61 537 Cuts at: 4388 4432 4445 4556 4595 5024 6008 6440 7187 Size: 44 13 111 39 429 984 432 747 Cuts at: 7187 7455 442 Size: 268 1170 Fragments arranged by size: 1170 984 747 656 639 537 432 429 410 366 324 296 268 202 186 111 96 79 61 49 44 39 26 18 13 1 EcoO109I rG'GnC_Cy Cuts at: 3840 4958 5322 3840 Size: 1118 364 6701 Fragments arranged by size: 6701 1118 364 EcoRI G'AATT_C Cuts at: 599 3300 5187 599 Size: 2701 1887 3595 Fragments arranged by size: 3595 2701 1887 EcoRV GAT'ATC Cuts at: 550 1344 2260 550 Size: 794 916 6473 Fragments arranged by size: 6473 916 794 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4846 6202 4846 Size: 1356 6827 FspI TGC'GCA Cuts at: 4938 5964 4938 Size: 1026 7157 HaeII r_GCGC'y Cuts at: 336 1929 5617 5865 6887 7866 336 Size: 1593 3688 248 1022 979 653 Fragments arranged by size: 3688 1593 1022 979 653 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1328 1333 1360 1365 1791 1823 2734 2766 3169 Size: 5 27 5 426 32 911 32 403 Cuts at: 3169 3201 3898 3930 4867 4899 6025 6055 6057 Size: 32 697 32 937 32 1126 30 2 Cuts at: 6057 6087 1328 Size: 30 3424 Fragments arranged by size: 3424 1126 937 911 697 426 403 32 32 32 32 32 30 30 27 5 5 2 HincII GTy'rAC Cuts at: 972 5222 972 Size: 4250 3933 HindIII A'AGCT_T Cuts at: 306 306 Size: 8183 HpaI GTT'AAC Cuts at: 972 972 Size: 8183 KasI G'GCGC_C Cuts at: 1925 5613 5861 1925 Size: 3688 248 4247 Fragments arranged by size: 4247 3688 248 KpnI G_GTAC'C Cuts at: 5203 5203 Size: 8183 MluI A'CGCG_T Cuts at: 7616 7616 Size: 8183 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1414 2239 2622 2798 4440 6854 7038 1414 Size: 825 383 176 1642 2414 184 2559 Fragments arranged by size: 2559 2414 1642 825 383 184 176 MscI TGG'CCA Cuts at: 3928 4762 5944 3928 Size: 834 1182 6167 Fragments arranged by size: 6167 1182 834 MslI CAynn'nnrTG Cuts at: 736 940 1484 2681 3261 3484 6172 6299 736 Size: 204 544 1197 580 223 2688 127 2620 Fragments arranged by size: 2688 2620 1197 580 544 223 204 127 MspA1I CmG'CkG Cuts at: 2505 3668 3713 4018 5294 5608 5968 6981 7226 Size: 1163 45 305 1276 314 360 1013 245 Cuts at: 7226 2505 Size: 3462 Fragments arranged by size: 3462 1276 1163 1013 360 314 305 245 45 NaeI GCC'GGC Cuts at: 6364 6364 Size: 8183 NarI GG'CG_CC Cuts at: 1926 5614 5862 1926 Size: 3688 248 4247 Fragments arranged by size: 4247 3688 248 NcoI C'CATG_G Cuts at: 107 2006 3479 107 Size: 1899 1473 4811 Fragments arranged by size: 4811 1899 1473 NdeI CA'TA_TG Cuts at: 612 1256 4164 4501 612 Size: 644 2908 337 4294 Fragments arranged by size: 4294 2908 644 337 NgoMIV G'CCGG_C Cuts at: 6362 6362 Size: 8183 NsiI A_TGCA'T Cuts at: 3533 3533 Size: 8183 NspI r_CATG'y Cuts at: 939 1452 2144 2156 2177 4278 4309 5236 5736 Size: 513 692 12 21 2101 31 927 500 Cuts at: 5736 6643 939 Size: 907 2479 Fragments arranged by size: 2479 2101 927 907 692 513 500 31 21 12 PciI A'CATG_T Cuts at: 2152 2173 4274 6639 2152 Size: 21 2101 2365 3696 Fragments arranged by size: 3696 2365 2101 21 PflMI CCAn_nnn'nTGG Cuts at: 275 2548 2919 3954 275 Size: 2273 371 1035 4504 Fragments arranged by size: 4504 2273 1035 371 PfoI T'CCnGG_A Cuts at: 449 1457 4469 449 Size: 1008 3012 4163 Fragments arranged by size: 4163 3012 1008 PmeI GTTT'AAAC Cuts at: 5182 5182 Size: 8183 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 558 590 7322 7354 558 Size: 32 6732 32 1387 Fragments arranged by size: 6732 1387 32 32 PspOMI G'GGCC_C Cuts at: 841 841 Size: 8183 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 1163 1195 1163 Size: 32 8151 PstI C_TGCA'G Cuts at: 1641 3848 4818 5230 5915 1641 Size: 2207 970 412 685 3909 Fragments arranged by size: 3909 2207 970 685 412 PvuI CG_AT'CG Cuts at: 104 104 Size: 8183 PvuII CAG'CTG Cuts at: 2505 3668 3713 4018 5608 5968 2505 Size: 1163 45 305 1590 360 4720 Fragments arranged by size: 4720 1590 1163 360 305 45 RsrII CG'GwC_CG Cuts at: 3338 6378 3338 Size: 3040 5143 SacI G_AGCT'C Cuts at: 2951 3644 5197 2951 Size: 693 1553 5937 Fragments arranged by size: 5937 1553 693 SalI G'TCGA_C Cuts at: 5220 5220 Size: 8183 SapI GCTCTTCn'nnn_ Cuts at: 4366 6206 6416 4366 Size: 1840 210 6133 Fragments arranged by size: 6133 1840 210 SbfI CC_TGCA'GG Cuts at: 5230 5230 Size: 8183 ScaI AGT'ACT Cuts at: 7700 7700 Size: 8183 SfcI C'TryA_G Cuts at: 33 1637 2941 2995 3631 3844 4814 5226 5911 Size: 1604 1304 54 636 213 970 412 685 Cuts at: 5911 6904 7095 33 Size: 993 191 1121 Fragments arranged by size: 1604 1304 1121 993 970 685 636 412 213 191 54 SfoI GGC'GCC Cuts at: 1927 5615 5863 1927 Size: 3688 248 4247 Fragments arranged by size: 4247 3688 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 8183 SmaI CCC'GGG Cuts at: 5205 5205 Size: 8183 SmlI C'TyrA_G Cuts at: 346 1151 3110 3175 3292 3971 4337 5338 6253 Size: 805 1959 65 117 679 366 1001 915 Cuts at: 6253 6745 7007 346 Size: 492 262 1522 Fragments arranged by size: 1959 1522 1001 915 805 679 492 366 262 117 65 SpeI A'CTAG_T Cuts at: 67 1272 67 Size: 1205 6978 SphI G_CATG'C Cuts at: 2144 4309 5236 5736 2144 Size: 2165 927 500 4591 Fragments arranged by size: 4591 2165 927 500 StuI AGG'CCT Cuts at: 2590 2590 Size: 8183 StyI C'CwwG_G Cuts at: 107 269 845 2006 3479 4354 4679 5317 5671 Size: 162 576 1161 1473 875 325 638 354 Cuts at: 5671 107 Size: 2619 Fragments arranged by size: 2619 1473 1161 875 638 576 354 325 162 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 634 2065 3021 3355 4541 5020 5766 5899 6358 Size: 1431 956 334 1186 479 746 133 459 Cuts at: 6358 6567 634 Size: 209 2250 Fragments arranged by size: 2250 1431 1186 956 746 479 459 334 209 133 TatI w'GTAC_w Cuts at: 1763 1867 2067 7698 1763 Size: 104 200 5631 2248 Fragments arranged by size: 5631 2248 200 104 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1361 1393 7700 7732 1361 Size: 32 6307 32 1812 Fragments arranged by size: 6307 1812 32 32 Tth111I GACn'n_nGTC Cuts at: 777 5980 777 Size: 5203 2980 XbaI T'CTAG_A Cuts at: 5214 5214 Size: 8183 XcmI CCAnnnn_n'nnnnTGG Cuts at: 114 366 1740 2571 3564 5678 114 Size: 252 1374 831 993 2114 2619 Fragments arranged by size: 2619 2114 1374 993 831 252 XhoI C'TCGA_G Cuts at: 1151 3110 3971 1151 Size: 1959 861 5363 Fragments arranged by size: 5363 1959 861 XmaI C'CCGG_G Cuts at: 5203 5203 Size: 8183 XmnI GAAnn'nnTTC Cuts at: 599 2111 2672 3137 4151 4241 599 Size: 1512 561 465 1014 90 4541 Fragments arranged by size: 4541 1512 1014 561 465 90 ZraI GAC'GTC Cuts at: 1894 1894 Size: 8183 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI PflMI PfoI PmeI PpiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AhdI AleI AloI AscI AvrII BaeI BcgI BglI BmtI BsmBI BssHII ClaI CspCI EcoNI FseI FspAI MfeI NheI NotI NruI PacI PasI PmlI PpuMI PshAI PsiI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI