Restriction map for
pF1KB0020
(Circular) (Six-base) MAPSORT of: pF1KB0020.seq Check: 6747 from: 1 to: 8462
pF1KB0020 8462 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4438 4438
Size: 8462
AatII G_ACGT'C
Cuts at: 4831 4831
Size: 8462
AccI GT'mk_AC
Cuts at: 1112 5500 1112
Size: 4388 4074
Acc65I G'GTAC_C
Cuts at: 3715 5478 3715
Size: 1763 6699
AclI AA'CG_TT
Cuts at: 4435 8302 4435
Size: 3867 4595
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 464 545 856 1061 1304 1460 1904 2840 2909
Size: 81 311 205 243 156 444 936 69
Cuts at: 2909 3116 3289 3371 3385 4028 4229 4651 4931
Size: 207 173 82 14 643 201 422 280
Cuts at: 4931 6287 6719 7466 7734 464
Size: 1356 432 747 268 1192
Fragments arranged by size:
1356 1192 936 747 643 444 432 422
311 280 268 243 207 205 201 173
156 82 81 69 14
AfeI AGC'GCT
Cuts at: 2028 2028
Size: 8462
AflII C'TTAA_G
Cuts at: 4593 4593
Size: 8462
AflIII A'CryG_T
Cuts at: 1513 1837 5814 6918 7895 1513
Size: 324 3977 1104 977 2080
Fragments arranged by size:
3977 2080 1104 977 324
AgeI A'CCGG_T
Cuts at: 3283 6850 3283
Size: 3567 4895
AleI CACnn'nnGTG
Cuts at: 2743 3169 4632 2743
Size: 426 1463 6573
Fragments arranged by size:
6573 1463 426
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3563 3595 3563
Size: 32 8430
AlwNI CAG_nnn'CTG
Cuts at: 742 1429 2059 2494 2740 2791 3049 3751 4378
Size: 687 630 435 246 51 258 702 627
Cuts at: 4378 5076 5455 5767 7334 742
Size: 698 379 312 1567 1870
Fragments arranged by size:
1870 1567 702 698 687 630 627 435
379 312 258 246 51
ApaI G_GGCC'C
Cuts at: 4538 4538
Size: 8462
ApaLI G'TGCA_C
Cuts at: 4451 5362 7232 4451
Size: 911 1870 5681
Fragments arranged by size:
5681 1870 911
ApoI r'AATT_y
Cuts at: 652 860 1000 4334 5466 7815 8071 652
Size: 208 140 3334 1132 2349 256 1043
Fragments arranged by size:
3334 2349 1132 1043 256 208 140
AseI AT'TA_AT
Cuts at: 20 20
Size: 8462
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8462
AvaI C'yCGr_G
Cuts at: 154 181 1825 2722 2987 5482 154
Size: 27 1644 897 265 2495 3134
Fragments arranged by size:
3134 2495 1644 897 265 27
AvrII C'CTAG_G
Cuts at: 4305 4305
Size: 8462
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2120 2153 2120
Size: 33 8429
BamHI G'GATC_C
Cuts at: 2288 5487 2288
Size: 3199 5263
BanI G'GyrC_C
Cuts at: 112 216 3064 3280 3715 5478 5892 6140 6175
Size: 104 2848 216 435 1763 414 248 35
Cuts at: 6175 112
Size: 2399
Fragments arranged by size:
2848 2399 1763 435 414 248 216 104
35
BanII G_rGCy'C
Cuts at: 124 166 182 188 329 369 387 1211 1804
Size: 42 16 6 141 40 18 824 593
Cuts at: 1804 1826 2759 4351 4538 5476 6506 124
Size: 22 933 1592 187 938 1030 2080
Fragments arranged by size:
2080 1592 1030 938 933 824 593 187
141 42 40 22 18 16 6
BbeI G_GCGC'C
Cuts at: 116 220 5896 6144 116
Size: 104 5676 248 2434
Fragments arranged by size:
5676 2434 248 104
BbsI GAAGACnn'nnnn_
Cuts at: 1130 2030 2456 2600 3833 4499 4523 1130
Size: 900 426 144 1233 666 24 5069
Fragments arranged by size:
5069 1233 900 666 426 144 24
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1786 1820 3070 3104 1786
Size: 34 1250 34 7144
Fragments arranged by size:
7144 1250 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2121 3882 6360 7121 8462 2121
Size: 1761 2478 761 1341 2121
Fragments arranged by size:
2478 2121 1761 1341 761
BclI T'GATC_A
Cuts at: 5067 5982 5067
Size: 915 7547
BglI GCCn_nnn'nGGC
Cuts at: 214 4533 214
Size: 4319 4143
BglII A'GATC_T
Cuts at: 5977 8014 5977
Size: 2037 6425
BlpI GC'TnA_GC
Cuts at: 176 314 328 1854 2758 5574 176
Size: 138 14 1526 904 2816 3064
Fragments arranged by size:
3064 2816 1526 904 138 14
Bme1580I G_kGCm'C
Cuts at: 1203 3733 4250 4455 4529 4538 5366 6087 6180
Size: 2530 517 205 74 9 828 721 93
Cuts at: 6180 7236 1203
Size: 1056 2429
Fragments arranged by size:
2530 2429 1056 828 721 517 205 93
74 9
BmgBI CAC'GTC
Cuts at: 1368 1368
Size: 8462
BmrI ACTGGGnnnn_n'
Cuts at: 3123 3714 5674 5755 6090 8234 3123
Size: 591 1960 81 335 2144 3351
Fragments arranged by size:
3351 2144 1960 591 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 180 1161 1263 1567 3239 4494 6829 180
Size: 981 102 304 1672 1255 2335 1813
Fragments arranged by size:
2335 1813 1672 1255 981 304 102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 794 2471 3220 3755 4216 319
Size: 475 1677 749 535 461 4565
Fragments arranged by size:
4565 1677 749 535 475 461
Bpu10I CC'TnA_GC
Cuts at: 3401 3537 3401
Size: 136 8326
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 995 3209 3464 3884 3979 4201 4756 5638 6517
Size: 2214 255 420 95 222 555 882 879
Cuts at: 6517 7009 7307 995
Size: 492 298 2150
Fragments arranged by size:
2214 2150 882 879 555 492 420 298
255 222 95
BsaI GGTCTCn'nnnn_
Cuts at: 823 2228 2526 2840 3094 4732 8166 823
Size: 1405 298 314 254 1638 3434 1119
Fragments arranged by size:
3434 1638 1405 1119 314 298 254
BsaAI yAC'GTr
Cuts at: 1838 4798 4854 5815 1838
Size: 2960 56 961 4485
Fragments arranged by size:
4485 2960 961 56
BsaBI GATnn'nnATC
Cuts at: 6 5976 8013 6
Size: 5970 2037 455
Fragments arranged by size:
5970 2037 455
BsaHI Gr'CG_yC
Cuts at: 113 217 1960 4828 5893 6141 6843 8339 113
Size: 104 1743 2868 1065 248 702 1496 236
Fragments arranged by size:
2868 1743 1496 1065 702 248 236 104
BsaWI w'CCGG_w
Cuts at: 1522 1770 3168 3283 5651 6172 6850 7124 7271
Size: 248 1398 115 2368 521 678 274 147
Cuts at: 7271 7682 1522
Size: 411 2302
Fragments arranged by size:
2368 2302 1398 678 521 411 274 248
147 115
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1822 1852 4951 4981 1822
Size: 30 3099 30 5303
Fragments arranged by size:
5303 3099 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 541 1267 1396 1733 1736 1739 1798 1834 1843
Size: 726 129 337 3 3 59 36 9
Cuts at: 1843 2246 2923 3172 3467 3709 4082 4219 4286
Size: 403 677 249 295 242 373 137 67
Cuts at: 4286 4330 4399 4714 4972 5419 541
Size: 44 69 315 258 447 3584
Fragments arranged by size:
3584 726 677 447 403 373 337 315
295 258 249 242 137 129 69 67
59 44 36 9 3 3
BseYI C'CCAG_C
Cuts at: 187 760 870 2461 3269 3541 5887 6645 7222
Size: 573 110 1591 808 272 2346 758 577
Cuts at: 7222 7718 187
Size: 496 931
Fragments arranged by size:
2346 1591 931 808 758 577 573 496
272 110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1660 1885 3193 3334 3737 4907 1660
Size: 225 1308 141 403 1170 5215
Fragments arranged by size:
5215 1308 1170 403 225 141
BsiEI CG_ry'CG
Cuts at: 104 209 2642 3002 6004 6050 7258 104
Size: 105 2433 360 3002 46 1208 1308
Fragments arranged by size:
3002 2433 1308 1208 360 105 46
BsiHKAI G_wGCw'C
Cuts at: 124 166 329 1748 1804 2053 2759 4187 4351
Size: 42 163 1419 56 249 706 1428 164
Cuts at: 4351 4455 5366 5476 6254 6444 7236 124
Size: 104 911 110 778 190 792 1350
Fragments arranged by size:
1428 1419 1350 911 792 778 706 249
190 164 163 110 104 56 42
BsmI GAATG_Cn'
Cuts at: 904 904
Size: 8462
BsmBI CGTCTCn'nnnn_
Cuts at: 1721 2266 3367 1721
Size: 545 1101 6816
Fragments arranged by size:
6816 1101 545
Bsp1286I G_dGCh'C
Cuts at: 124 166 182 188 329 369 387 1203 1211
Size: 42 16 6 141 40 18 816 8
Cuts at: 1211 1748 1804 1826 2053 2759 3733 4187 4250
Size: 537 56 22 227 706 974 454 63
Cuts at: 4250 4351 4455 4529 4538 5366 5476 6087 6180
Size: 101 104 74 9 828 110 611 93
Cuts at: 6180 6254 6444 6506 7236 124
Size: 74 190 62 730 1350
Fragments arranged by size:
1350 974 828 816 730 706 611 537
454 227 190 141 110 104 101 93
74 74 63 62 56 42 40 22
18 16 9 8 6
BspEI T'CCGG_A
Cuts at: 1522 1770 7682 1522
Size: 248 5912 2302
Fragments arranged by size:
5912 2302 248
BspHI T'CATG_A
Cuts at: 7638 7638
Size: 8462
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1302 1491 4438 4650 5512 5726 6028 6409 1302
Size: 189 2947 212 862 214 302 381 3355
Fragments arranged by size:
3355 2947 862 381 302 214 212 189
BsrBI CCG'CTC
Cuts at: 50 4504 6754 6808 8292 8457 50
Size: 4454 2250 54 1484 165 55
Fragments arranged by size:
4454 2250 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2231 6374 2231
Size: 4143 4319
BsrFI r'CCGG_y
Cuts at: 171 1021 1188 3283 6460 6641 6850 171
Size: 850 167 2095 3177 181 209 1783
Fragments arranged by size:
3177 2095 1783 850 209 181 167
BssSI C'ACGA_G
Cuts at: 1204 6733 7091 1204
Size: 5529 358 2575
Fragments arranged by size:
5529 2575 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1141 1198 3049 3091 3751 5839 1141
Size: 57 1851 42 660 2088 3764
Fragments arranged by size:
3764 2088 1851 660 57 42
BstBI TT'CG_AA
Cuts at: 3950 6823 3950
Size: 2873 5589
BstEII G'GTnAC_C
Cuts at: 343 343
Size: 8462
BstXI CCAn_nnnn'nTGG
Cuts at: 1494 3111 3277 4879 1494
Size: 1617 166 1602 5077
Fragments arranged by size:
5077 1617 1602 166
BstYI r'GATC_y
Cuts at: 1 2288 5487 5954 5977 6312 6558 7570 7656
Size: 2287 3199 467 23 335 246 1012 86
Cuts at: 7656 7668 8014 1
Size: 12 346 449
Fragments arranged by size:
3199 2287 1012 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1469 1817 4913 5268 1469
Size: 348 3096 355 4663
Fragments arranged by size:
4663 3096 355 348
BtgI C'CryG_G
Cuts at: 107 230 2642 2951 4103 107
Size: 123 2412 309 1152 4466
Fragments arranged by size:
4466 2412 1152 309 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 228 6412 6593 6756 228
Size: 6184 181 163 1934
Fragments arranged by size:
6184 1934 181 163
BtsI GCAGTG_nn'
Cuts at: 896 3248 4982 5705 896
Size: 2352 1734 723 3653
Fragments arranged by size:
3653 2352 1734 723
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 4958 4993 4958
Size: 35 8427
DraI TTT'AAA
Cuts at: 651 2394 5461 651
Size: 1743 3067 3652
Fragments arranged by size:
3652 3067 1743
DrdI GACnn_nn'nnGTC
Cuts at: 2632 6168 7026 2632
Size: 3536 858 4068
Fragments arranged by size:
4068 3536 858
EaeI y'GGCC_r
Cuts at: 206 354 882 1355 2639 6047 6221 6612 6639
Size: 148 528 473 1284 3408 174 391 27
Cuts at: 6639 7984 206
Size: 1345 684
Fragments arranged by size:
3408 1345 1284 684 528 473 391 174
148 27
EagI C'GGCC_G
Cuts at: 206 2639 6047 206
Size: 2433 3408 2621
Fragments arranged by size:
3408 2621 2433
EarI CTCTTCn'nnn_
Cuts at: 572 821 911 1280 1340 1388 2087 2202 2432
Size: 249 90 369 60 48 699 115 230
Cuts at: 2432 3092 3200 3335 4166 4205 4463 5075 5099
Size: 660 108 135 831 39 258 612 24
Cuts at: 5099 6485 6695 572
Size: 1386 210 2339
Fragments arranged by size:
2339 1386 831 699 660 612 369 258
249 230 210 135 115 108 90 60
48 39 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3028 3234 6670 6980 7126 8272 3028
Size: 206 3436 310 146 1146 3218
Fragments arranged by size:
3436 3218 1146 310 206 146
EcoICRI GAG'CTC
Cuts at: 122 164 327 1802 2757 4349 5474 122
Size: 42 163 1475 955 1592 1125 3110
Fragments arranged by size:
3110 1592 1475 1125 955 163 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 464 545 794 856 1061 1304 1460 1904
Size: 145 81 249 62 205 243 156 444
Cuts at: 1904 2471 2840 2909 3116 3220 3289 3371 3385
Size: 567 369 69 207 104 69 82 14
Cuts at: 3385 3755 4028 4216 4229 4651 4931 6287 6719
Size: 370 273 188 13 422 280 1356 432
Cuts at: 6719 7466 7734 319
Size: 747 268 1047
Fragments arranged by size:
1356 1047 747 567 444 432 422 370
369 280 273 268 249 243 207 205
188 156 145 104 82 81 69 69
62 14 13
EcoNI CCTnn'n_nnAGG
Cuts at: 337 2360 337
Size: 2023 6439
EcoO109I rG'GnC_Cy
Cuts at: 756 1276 1530 2192 3231 4534 4908 5601 756
Size: 520 254 662 1039 1303 374 693 3617
Fragments arranged by size:
3617 1303 1039 693 662 520 374 254
EcoRI G'AATT_C
Cuts at: 5466 5466
Size: 8462
EcoRV GAT'ATC
Cuts at: 2849 2849
Size: 8462
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2855 3319 4033 4195 4608 6481 2855
Size: 464 714 162 413 1873 4836
Fragments arranged by size:
4836 1873 714 464 413 162
FspI TGC'GCA
Cuts at: 6243 6243
Size: 8462
HaeII r_GCGC'y
Cuts at: 116 220 2030 5896 6144 7166 8145 116
Size: 104 1810 3866 248 1022 979 433
Fragments arranged by size:
3866 1810 1022 979 433 248 104
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1720 1752 1824 1856 1910 1942 2853 2885 3694
Size: 32 72 32 54 32 911 32 809
Cuts at: 3694 3726 4833 4865 4951 4983 5413 5445 6304
Size: 32 1107 32 86 32 430 32 859
Cuts at: 6304 6334 6336 6366 1720
Size: 30 2 30 3816
Fragments arranged by size:
3816 1107 911 859 809 430 86 72
54 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2903 3134 4785 4826 4970 5501 2903
Size: 231 1651 41 144 531 5864
Fragments arranged by size:
5864 1651 531 231 144 41
HindIII A'AGCT_T
Cuts at: 1073 1660 1687 2147 3462 4177 1073
Size: 587 27 460 1315 715 5358
Fragments arranged by size:
5358 1315 715 587 460 27
HpaI GTT'AAC
Cuts at: 2903 3134 2903
Size: 231 8231
KasI G'GCGC_C
Cuts at: 112 216 5892 6140 112
Size: 104 5676 248 2434
Fragments arranged by size:
5676 2434 248 104
KpnI G_GTAC'C
Cuts at: 3719 5482 3719
Size: 1763 6699
MfeI C'AATT_G
Cuts at: 4694 4694
Size: 8462
MluI A'CGCG_T
Cuts at: 7895 7895
Size: 8462
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 408 982 4418 4625 7133 7317 408
Size: 574 3436 207 2508 184 1553
Fragments arranged by size:
3436 2508 1553 574 207 184
MscI TGG'CCA
Cuts at: 356 884 6223 356
Size: 528 5339 2595
Fragments arranged by size:
5339 2595 528
MslI CAynn'nnrTG
Cuts at: 2743 3109 3169 4166 4632 4819 6451 6578 2743
Size: 366 60 997 466 187 1632 127 4627
Fragments arranged by size:
4627 1632 997 466 366 187 127 60
MspA1I CmG'CkG
Cuts at: 191 232 291 493 1222 1438 2791 3040 3273
Size: 41 59 202 729 216 1353 249 233
Cuts at: 3273 4195 4375 5076 5573 5887 6247 7260 7505
Size: 922 180 701 497 314 360 1013 245
Cuts at: 7505 191
Size: 1148
Fragments arranged by size:
1353 1148 1013 922 729 701 497 360
314 249 245 233 216 202 180 59
41
NaeI GCC'GGC
Cuts at: 173 1023 1190 6643 173
Size: 850 167 5453 1992
Fragments arranged by size:
5453 1992 850 167
NarI GG'CG_CC
Cuts at: 113 217 5893 6141 113
Size: 104 5676 248 2434
Fragments arranged by size:
5676 2434 248 104
NcoI C'CATG_G
Cuts at: 107 107
Size: 8462
NdeI CA'TA_TG
Cuts at: 2777 2777
Size: 8462
NgoMIV G'CCGG_C
Cuts at: 171 1021 1188 6641 171
Size: 850 167 5453 1992
Fragments arranged by size:
5453 1992 850 167
NspI r_CATG'y
Cuts at: 5515 6015 6922 5515
Size: 500 907 7055
Fragments arranged by size:
7055 907 500
PasI CC'CwG_GG
Cuts at: 1934 1934
Size: 8462
PciI A'CATG_T
Cuts at: 6918 6918
Size: 8462
PflMI CCAn_nnn'nTGG
Cuts at: 891 3243 891
Size: 2352 6110
PfoI T'CCnGG_A
Cuts at: 335 2541 3794 335
Size: 2206 1253 5003
Fragments arranged by size:
5003 2206 1253
PmeI GTTT'AAAC
Cuts at: 5461 5461
Size: 8462
PmlI CAC'GTG
Cuts at: 1838 4854 1838
Size: 3016 5446
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7601 7633 7601
Size: 32 8430
PpuMI rG'GwC_Cy
Cuts at: 756 1276 1530 2192 3231 4908 756
Size: 520 254 662 1039 1677 4310
Fragments arranged by size:
4310 1677 1039 662 520 254
PshAI GACnn'nnGTC
Cuts at: 1553 1553
Size: 8462
PspOMI G'GGCC_C
Cuts at: 4534 4534
Size: 8462
PstI C_TGCA'G
Cuts at: 951 3045 3054 4076 4383 4449 5509 6194 951
Size: 2094 9 1022 307 66 1060 685 3219
Fragments arranged by size:
3219 2094 1060 1022 685 307 66 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8462
PvuII CAG'CTG
Cuts at: 291 1222 1438 2791 3040 3273 4195 5076 5887
Size: 931 216 1353 249 233 922 881 811
Cuts at: 5887 6247 291
Size: 360 2506
Fragments arranged by size:
2506 1353 931 922 881 811 360 249
233 216
RsrII CG'GwC_CG
Cuts at: 6657 6657
Size: 8462
SacI G_AGCT'C
Cuts at: 124 166 329 1804 2759 4351 5476 124
Size: 42 163 1475 955 1592 1125 3110
Fragments arranged by size:
3110 1592 1475 1125 955 163 42
SacII CC_GC'GG
Cuts at: 233 233
Size: 8462
SalI G'TCGA_C
Cuts at: 5499 5499
Size: 8462
SapI GCTCTTCn'nnn_
Cuts at: 572 1388 2087 3200 3335 6485 6695 572
Size: 816 699 1113 135 3150 210 2339
Fragments arranged by size:
3150 2339 1113 816 699 210 135
SbfI CC_TGCA'GG
Cuts at: 4449 5509 4449
Size: 1060 7402
ScaI AGT'ACT
Cuts at: 7979 7979
Size: 8462
SfcI C'TryA_G
Cuts at: 33 274 812 947 2669 3041 3050 3605 3968
Size: 241 538 135 1722 372 9 555 363
Cuts at: 3968 4072 4379 4445 5505 6190 7183 7374 33
Size: 104 307 66 1060 685 993 191 1121
Fragments arranged by size:
1722 1121 1060 993 685 555 538 372
363 307 241 191 135 104 66 9
SfoI GGC'GCC
Cuts at: 114 218 5894 6142 114
Size: 104 5676 248 2434
Fragments arranged by size:
5676 2434 248 104
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8462
SgrAI Cr'CCGG_yG
Cuts at: 3283 3283
Size: 8462
SmaI CCC'GGG
Cuts at: 2989 5484 2989
Size: 2495 5967
SmlI C'TyrA_G
Cuts at: 1010 3224 3479 3899 3958 4180 4593 4735 5617
Size: 2214 255 420 59 222 413 142 882
Cuts at: 5617 6532 7024 7286 1010
Size: 915 492 262 2186
Fragments arranged by size:
2214 2186 915 882 492 420 413 262
255 222 142 59
SnaBI TAC'GTA
Cuts at: 4798 4798
Size: 8462
SpeI A'CTAG_T
Cuts at: 67 961 5184 67
Size: 894 4223 3345
Fragments arranged by size:
4223 3345 894
SphI G_CATG'C
Cuts at: 5515 6015 5515
Size: 500 7962
StuI AGG'CCT
Cuts at: 3768 4918 3768
Size: 1150 7312
StyI C'CwwG_G
Cuts at: 107 765 1487 2750 4305 5596 5950 107
Size: 658 722 1263 1555 1291 354 2619
Fragments arranged by size:
2619 1555 1291 1263 722 658 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1193 3052 6045 6178 6637 6846 1193
Size: 1859 2993 133 459 209 2809
Fragments arranged by size:
2993 2809 1859 459 209 133
TatI w'GTAC_w
Cuts at: 7977 7977
Size: 8462
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1740 1772 1822 1854 7979 8011 1740
Size: 32 50 32 6125 32 2191
Fragments arranged by size:
6125 2191 50 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 691 2017 2998 6259 691
Size: 1326 981 3261 2894
Fragments arranged by size:
3261 2894 1326 981
XbaI T'CTAG_A
Cuts at: 3834 5493 3834
Size: 1659 6803
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5957 5957
Size: 8462
XmaI C'CCGG_G
Cuts at: 2987 5482 2987
Size: 2495 5967
XmnI GAAnn'nnTTC
Cuts at: 551 551
Size: 8462
ZraI GAC'GTC
Cuts at: 4829 4829
Size: 8462
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AhdI AlfI AscI BbvCI BfrBI BmtI BsiWI BsrGI
BssHII BstZ17I ClaI DraIII FseI FspAI NheI NotI
NruI NsiI PacI PsiI PspXI PsrI SanDI SexAI
SfiI SrfI SspI SwaI XhoI