Restriction map for
pF1KB0026
(Circular) (Six-base) MAPSORT of: pF1KB0026.seq Check: 5897 from: 1 to: 6287
pF1KB0026 6287 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 975 1996 975
Size: 1021 5266
AatII G_ACGT'C
Cuts at: 2771 2771
Size: 6287
AccI GT'mk_AC
Cuts at: 3325 3325
Size: 6287
Acc65I G'GTAC_C
Cuts at: 3303 3303
Size: 6287
AclI AA'CG_TT
Cuts at: 6127 6127
Size: 6287
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1030 1054 1777 1927 2567 4112 4544 4897 5559
Size: 24 723 150 640 1545 432 353 662
Cuts at: 5559 1030
Size: 1758
Fragments arranged by size:
1758 1545 723 662 640 432 353 150
24
AfeI AGC'GCT
Cuts at: 875 875
Size: 6287
AflII C'TTAA_G
Cuts at: 826 826
Size: 6287
AflIII A'CryG_T
Cuts at: 3639 5439 5720 3639
Size: 1800 281 4206
Fragments arranged by size:
4206 1800 281
AleI CACnn'nnGTG
Cuts at: 1414 2437 3023 1414
Size: 1023 586 4678
Fragments arranged by size:
4678 1023 586
AlwNI CAG_nnn'CTG
Cuts at: 288 1320 1717 2247 2304 2385 2478 2826 3592
Size: 1032 397 530 57 81 93 348 766
Cuts at: 3592 5030 288
Size: 1438 1545
Fragments arranged by size:
1545 1438 1032 766 530 397 348 93
81 57
ApaI G_GGCC'C
Cuts at: 1950 1950
Size: 6287
ApaLI G'TGCA_C
Cuts at: 838 2389 5125 838
Size: 1551 2736 2000
Fragments arranged by size:
2736 2000 1551
ApoI r'AATT_y
Cuts at: 376 1733 2359 3291 5640 5896 376
Size: 1357 626 932 2349 256 767
Fragments arranged by size:
2349 1357 932 767 626 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6287
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6287
AvaI C'yCGr_G
Cuts at: 1375 1899 2013 3307 1375
Size: 524 114 1294 4355
Fragments arranged by size:
4355 1294 524 114
BamHI G'GATC_C
Cuts at: 1811 3312 1811
Size: 1501 4786
BanI G'GyrC_C
Cuts at: 1580 1653 2008 2118 2887 3303 3717 3965 4000
Size: 73 355 110 769 416 414 248 35
Cuts at: 4000 1580
Size: 3867
Fragments arranged by size:
3867 769 416 414 355 248 110 73
35
BanII G_rGCy'C
Cuts at: 883 1199 1535 1630 1682 1950 2020 2942 3129
Size: 316 336 95 52 268 70 922 187
Cuts at: 3129 3301 4331 883
Size: 172 1030 2839
Fragments arranged by size:
2839 1030 922 336 316 268 187 172
95 70 52
BbeI G_GCGC'C
Cuts at: 1584 2122 3721 3969 1584
Size: 538 1599 248 3902
Fragments arranged by size:
3902 1599 538 248
BbsI GAAGACnn'nnnn_
Cuts at: 1561 2862 1561
Size: 1301 4986
BbvCI CC'TCA_GC
Cuts at: 1263 2266 2578 1263
Size: 1003 312 4972
Fragments arranged by size:
4972 1003 312
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1438 1472 1994 2028 2667 2701 1438
Size: 34 522 34 639 34 5024
Fragments arranged by size:
5024 639 522 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4185 5241 6287 4185
Size: 1056 1046 4185
Fragments arranged by size:
4185 1056 1046
BclI T'GATC_A
Cuts at: 242 290 1571 3807 242
Size: 48 1281 2236 2722
Fragments arranged by size:
2722 2236 1281 48
BglI GCCn_nnn'nGGC
Cuts at: 1999 2885 1999
Size: 886 5401
BglII A'GATC_T
Cuts at: 1271 3802 5839 1271
Size: 2531 2037 1719
Fragments arranged by size:
2531 2037 1719
BlpI GC'TnA_GC
Cuts at: 3399 3399
Size: 6287
Bme1580I G_kGCm'C
Cuts at: 842 1096 1950 2142 2393 2890 3265 3912 4005
Size: 254 854 192 251 497 375 647 93
Cuts at: 4005 5129 842
Size: 1124 2000
Fragments arranged by size:
2000 1124 854 647 497 375 254 251
192 93
BmgBI CAC'GTC
Cuts at: 1752 1752
Size: 6287
BmrI ACTGGGnnnn_n'
Cuts at: 1501 3499 3580 3915 6059 1501
Size: 1998 81 335 2144 1729
Fragments arranged by size:
2144 1998 1729 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2925 4654 2925
Size: 1729 4558
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1213 1663 1954 2134 2164 2821 2954 3006 3151
Size: 450 291 180 30 657 133 52 145
Cuts at: 3151 1213
Size: 4349
Fragments arranged by size:
4349 657 450 291 180 145 133 52
30
Bpu10I CC'TnA_GC
Cuts at: 1263 1624 2266 2578 1263
Size: 361 642 312 4972
Fragments arranged by size:
4972 642 361 312
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 152 480 1162 3463 4342 5056 5354 152
Size: 328 682 2301 879 714 298 1085
Fragments arranged by size:
2301 1085 879 714 682 328 298
BsaI GGTCTCn'nnnn_
Cuts at: 900 2201 5991 900
Size: 1301 3790 1196
Fragments arranged by size:
3790 1301 1196
BsaAI yAC'GTr
Cuts at: 3640 3640
Size: 6287
BsaBI GATnn'nnATC
Cuts at: 6 3801 5838 6
Size: 3795 2037 455
Fragments arranged by size:
3795 2037 455
BsaHI Gr'CG_yC
Cuts at: 723 1327 1581 2119 2768 3043 3718 3966 4668
Size: 604 254 538 649 275 675 248 702
Cuts at: 4668 6164 723
Size: 1496 846
Fragments arranged by size:
1496 846 702 675 649 604 538 275
254 248
BsaWI w'CCGG_w
Cuts at: 3476 3997 4675 5086 5233 5507 3476
Size: 521 678 411 147 274 4256
Fragments arranged by size:
4256 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 116 146 1153 1183 1906 1936 2254 2284 2950
Size: 30 1007 30 723 30 318 30 666
Cuts at: 2950 2980 116
Size: 30 3423
Fragments arranged by size:
3423 1007 723 666 318 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 187 412 843 1543 1555 1683 2248 2473 3158
Size: 225 431 700 12 128 565 225 685
Cuts at: 3158 3184 187
Size: 26 3290
Fragments arranged by size:
3290 700 685 565 431 225 225 128
26 12
BseYI C'CCAG_C
Cuts at: 509 578 876 1794 2883 2891 3712 4470 5135
Size: 69 298 918 1089 8 821 758 665
Cuts at: 5135 5543 509
Size: 408 1253
Fragments arranged by size:
1253 1089 918 821 758 665 408 298
69 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1000 2671 1000
Size: 1671 4616
BsiEI CG_ry'CG
Cuts at: 104 2537 3829 3875 5105 104
Size: 2433 1292 46 1230 1286
Fragments arranged by size:
2433 1292 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 272 842 1630 2393 2792 2942 3129 3301 4079
Size: 570 788 763 399 150 187 172 778
Cuts at: 4079 4269 5129 272
Size: 190 860 1430
Fragments arranged by size:
1430 860 788 778 763 570 399 190
187 172 150
BsmI GAATG_Cn'
Cuts at: 343 343
Size: 6287
BsmBI CGTCTCn'nnnn_
Cuts at: 1747 1747
Size: 6287
Bsp1286I G_dGCh'C
Cuts at: 272 842 883 1096 1199 1535 1630 1682 1950
Size: 570 41 213 103 336 95 52 268
Cuts at: 1950 2020 2142 2393 2792 2890 2942 3129 3265
Size: 70 122 251 399 98 52 187 136
Cuts at: 3265 3301 3912 4005 4079 4269 4331 5129 272
Size: 36 611 93 74 190 62 798 1430
Fragments arranged by size:
1430 798 611 570 399 336 268 251
213 190 187 136 122 103 98 95
93 74 70 62 52 52 41 36
BspHI T'CATG_A
Cuts at: 4719 4719
Size: 6287
BspMI ACCTGCnnnn'nnnn_
Cuts at: 968 975 1996 2255 2384 3263 3337 3551 3853
Size: 7 1021 259 129 879 74 214 302
Cuts at: 3853 4234 968
Size: 381 3021
Fragments arranged by size:
3021 1021 879 381 302 259 214 129
74 7
BsrBI CCG'CTC
Cuts at: 50 116 4579 4633 6117 6282 50
Size: 66 4463 54 1484 165 55
Fragments arranged by size:
4463 1484 165 66 55 54
BsrDI GCAATG_nn'
Cuts at: 437 4199 437
Size: 3762 2525
BsrFI r'CCGG_y
Cuts at: 4285 4466 4285
Size: 181 6106
BsrGI T'GTAC_A
Cuts at: 2540 2540
Size: 6287
BssSI C'ACGA_G
Cuts at: 758 2676 4558 5266 758
Size: 1918 1882 708 1779
Fragments arranged by size:
1918 1882 1779 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1320 2162 3664 1320
Size: 842 1502 3943
Fragments arranged by size:
3943 1502 842
BstBI TT'CG_AA
Cuts at: 4648 4648
Size: 6287
BstXI CCAn_nnnn'nTGG
Cuts at: 315 876 1080 1611 1794 2089 3025 3030 315
Size: 561 204 531 183 295 936 5 3572
Fragments arranged by size:
3572 936 561 531 295 204 183 5
BstYI r'GATC_y
Cuts at: 1 219 1271 1811 1868 2093 2742 3312 3779
Size: 218 1052 540 57 225 649 570 467
Cuts at: 3779 3802 4137 4383 4689 4701 4787 5839 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 1052 649 570 540 467 449 335
306 246 225 218 86 57 23 12
Bsu36I CC'TnA_GG
Cuts at: 1100 1100
Size: 6287
BtgI C'CryG_G
Cuts at: 107 596 1415 107
Size: 489 819 4979
Fragments arranged by size:
4979 819 489
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 324 1172 2538 4237 4418 4581 324
Size: 848 1366 1699 181 163 2030
Fragments arranged by size:
2030 1699 1366 848 181 163
BtsI GCAGTG_nn'
Cuts at: 126 538 999 2479 3530 126
Size: 412 461 1480 1051 2883
Fragments arranged by size:
2883 1480 1051 461 412
ClaI AT'CG_AT
Cuts at: 188 188
Size: 6287
DraI TTT'AAA
Cuts at: 3286 3286
Size: 6287
DraIII CAC_nnn'GTG
Cuts at: 3275 3275
Size: 6287
DrdI GACnn_nn'nnGTC
Cuts at: 3993 5337 3993
Size: 1344 4943
EaeI y'GGCC_r
Cuts at: 974 1278 1466 1874 2000 2534 3872 4046 4437
Size: 304 188 408 126 534 1338 174 391
Cuts at: 4437 4464 5809 974
Size: 27 1345 1452
Fragments arranged by size:
1452 1345 1338 534 408 391 304 188
174 126 27
EagI C'GGCC_G
Cuts at: 2534 3872 2534
Size: 1338 4949
EarI CTCTTCn'nnn_
Cuts at: 791 1643 3176 4310 4520 791
Size: 852 1533 1134 210 2558
Fragments arranged by size:
2558 1533 1134 852 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1927 2038 4495 5237 5383 6097 1927
Size: 111 2457 742 146 714 2117
Fragments arranged by size:
2457 2117 742 714 146 111
EcoICRI GAG'CTC
Cuts at: 1628 2940 3127 3299 1628
Size: 1312 187 172 4616
Fragments arranged by size:
4616 1312 187 172
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1030 1054 1213 1663 1777 1927 1954 2134 2164
Size: 24 159 450 114 150 27 180 30
Cuts at: 2164 2567 2821 2954 3006 3151 4112 4544 4897
Size: 403 254 133 52 145 961 432 353
Cuts at: 4897 5559 1030
Size: 662 1758
Fragments arranged by size:
1758 961 662 450 432 403 353 254
180 159 150 145 133 114 52 30
27 24
EcoNI CCTnn'n_nnAGG
Cuts at: 207 1207 1375 207
Size: 1000 168 5119
Fragments arranged by size:
5119 1000 168
EcoO109I rG'GnC_Cy
Cuts at: 672 1250 1917 2301 2823 3054 3426 672
Size: 578 667 384 522 231 372 3533
Fragments arranged by size:
3533 667 578 522 384 372 231
EcoRI G'AATT_C
Cuts at: 3291 3291
Size: 6287
EcoRV GAT'ATC
Cuts at: 738 738
Size: 6287
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 828 2886 4306 828
Size: 2058 1420 2809
Fragments arranged by size:
2809 2058 1420
FspI TGC'GCA
Cuts at: 4068 4068
Size: 6287
HaeII r_GCGC'y
Cuts at: 241 877 1584 1718 1802 2122 2226 3010 3721
Size: 636 707 134 84 320 104 784 711
Cuts at: 3721 3969 5199 5970 241
Size: 248 1230 771 558
Fragments arranged by size:
1230 784 771 711 707 636 558 320
248 134 104 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 116 148 574 606 1153 1185 1906 1938 2198
Size: 32 426 32 547 32 721 32 260
Cuts at: 2198 2230 2254 2286 2971 3003 3091 3123 4129
Size: 32 24 32 685 32 88 32 1006
Cuts at: 4129 4159 4161 4191 116
Size: 30 2 30 2212
Fragments arranged by size:
2212 1006 721 685 547 426 260 88
32 32 32 32 32 32 32 32
30 30 24 2
HincII GTy'rAC
Cuts at: 3326 3326
Size: 6287
KasI G'GCGC_C
Cuts at: 1580 2118 3717 3965 1580
Size: 538 1599 248 3902
Fragments arranged by size:
3902 1599 538 248
KpnI G_GTAC'C
Cuts at: 3307 3307
Size: 6287
MluI A'CGCG_T
Cuts at: 5720 5720
Size: 6287
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 121 5046 5230 121
Size: 4925 184 1178
Fragments arranged by size:
4925 1178 184
MscI TGG'CCA
Cuts at: 976 1468 1876 4048 976
Size: 492 408 2172 3215
Fragments arranged by size:
3215 2172 492 408
MslI CAynn'nnrTG
Cuts at: 916 1360 1414 1474 1609 2437 2785 2857 3023
Size: 444 54 60 135 828 348 72 166
Cuts at: 3023 3068 4276 4403 916
Size: 45 1208 127 2800
Fragments arranged by size:
2800 1208 828 444 348 166 135 127
72 60 54 45
MspA1I CmG'CkG
Cuts at: 822 1303 1483 1844 2340 2925 3398 3712 4072
Size: 481 180 361 496 585 473 314 360
Cuts at: 4072 4856 5101 822
Size: 784 245 2008
Fragments arranged by size:
2008 784 585 496 481 473 361 360
314 245 180
NaeI GCC'GGC
Cuts at: 4468 4468
Size: 6287
NarI GG'CG_CC
Cuts at: 1581 2119 3718 3966 1581
Size: 538 1599 248 3902
Fragments arranged by size:
3902 1599 538 248
NcoI C'CATG_G
Cuts at: 107 596 107
Size: 489 5798
NgoMIV G'CCGG_C
Cuts at: 4466 4466
Size: 6287
NspI r_CATG'y
Cuts at: 407 456 955 1125 2196 2254 2325 3073 3340
Size: 49 499 170 1071 58 71 748 267
Cuts at: 3340 3840 5443 407
Size: 500 1603 1251
Fragments arranged by size:
1603 1251 1071 748 500 499 267 170
71 58 49
PasI CC'CwG_GG
Cuts at: 1593 1593
Size: 6287
PciI A'CATG_T
Cuts at: 5439 5439
Size: 6287
PflMI CCAn_nnn'nTGG
Cuts at: 2529 2529
Size: 6287
PfoI T'CCnGG_A
Cuts at: 281 326 1931 2738 281
Size: 45 1605 807 3830
Fragments arranged by size:
3830 1605 807 45
PmeI GTTT'AAAC
Cuts at: 3286 3286
Size: 6287
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4733 4765 4733
Size: 32 6255
PpuMI rG'GwC_Cy
Cuts at: 1250 1917 3054 1250
Size: 667 1137 4483
Fragments arranged by size:
4483 1137 667
PshAI GACnn'nnGTC
Cuts at: 2671 3059 2671
Size: 388 5899
PspOMI G'GGCC_C
Cuts at: 1946 1946
Size: 6287
PstI C_TGCA'G
Cuts at: 533 1316 1843 2069 2381 3334 4019 533
Size: 783 527 226 312 953 685 2801
Fragments arranged by size:
2801 953 783 685 527 312 226
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6287
PvuII CAG'CTG
Cuts at: 822 1303 1483 2340 3712 4072 822
Size: 481 180 857 1372 360 3037
Fragments arranged by size:
3037 1372 857 481 360 180
RsrII CG'GwC_CG
Cuts at: 4482 4482
Size: 6287
SacI G_AGCT'C
Cuts at: 1630 2942 3129 3301 1630
Size: 1312 187 172 4616
Fragments arranged by size:
4616 1312 187 172
SalI G'TCGA_C
Cuts at: 3324 3324
Size: 6287
SapI GCTCTTCn'nnn_
Cuts at: 4310 4520 4310
Size: 210 6077
SbfI CC_TGCA'GG
Cuts at: 2069 3334 2069
Size: 1265 5022
ScaI AGT'ACT
Cuts at: 2161 5804 2161
Size: 3643 2644
SexAI A'CCwGG_T
Cuts at: 1497 1497
Size: 6287
SfcI C'TryA_G
Cuts at: 33 529 565 1312 1839 2065 2377 3330 4015
Size: 496 36 747 527 226 312 953 685
Cuts at: 4015 4983 5174 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 953 747 685 527 496 312
226 191 36
SfoI GGC'GCC
Cuts at: 1582 2120 3719 3967 1582
Size: 538 1599 248 3902
Fragments arranged by size:
3902 1599 538 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6287
SmaI CCC'GGG
Cuts at: 3309 3309
Size: 6287
SmlI C'TyrA_G
Cuts at: 167 459 826 1177 3442 4357 5071 5333 167
Size: 292 367 351 2265 915 714 262 1121
Fragments arranged by size:
2265 1121 915 714 367 351 292 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6287
SphI G_CATG'C
Cuts at: 407 955 3340 3840 407
Size: 548 2385 500 2854
Fragments arranged by size:
2854 2385 548 500
StuI AGG'CCT
Cuts at: 574 937 1261 574
Size: 363 324 5600
Fragments arranged by size:
5600 363 324
StyI C'CwwG_G
Cuts at: 107 596 812 1049 1535 2102 2654 3421 3775
Size: 489 216 237 486 567 552 767 354
Cuts at: 3775 107
Size: 2619
Fragments arranged by size:
2619 767 567 552 489 486 354 237
216
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 664 2905 3080 3870 4003 4462 4671 664
Size: 2241 175 790 133 459 209 2280
Fragments arranged by size:
2280 2241 790 459 209 175 133
TatI w'GTAC_w
Cuts at: 779 1985 2159 2540 5802 779
Size: 1206 174 381 3262 1264
Fragments arranged by size:
3262 1264 1206 381 174
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1030 1062 5804 5836 1030
Size: 32 4742 32 1481
Fragments arranged by size:
4742 1481 32 32
Tth111I GACn'n_nGTC
Cuts at: 130 2704 4084 130
Size: 2574 1380 2333
Fragments arranged by size:
2574 2333 1380
XbaI T'CTAG_A
Cuts at: 3318 3318
Size: 6287
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 663 1080 1611 1638 2109 3782 663
Size: 417 531 27 471 1673 3168
Fragments arranged by size:
3168 1673 531 471 417 27
XmaI C'CCGG_G
Cuts at: 3307 3307
Size: 6287
XmnI GAAnn'nnTTC
Cuts at: 1735 1735
Size: 6287
ZraI GAC'GTC
Cuts at: 2769 2769
Size: 6287
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AgeI AhdI AlfI AloI AscI AvrII BaeI BfrBI
BmtI BsiWI BspEI BssHII BstEII BstZ17I CspCI FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PsiI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI