Restriction map for
pF1KB0038
(Circular) (Six-base) MAPSORT of: pF1KB0038.seq Check: 6401 from: 1 to: 4280
pF1KB0038 4280 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 538 538
Size: 4280
AccI GT'mk_AC
Cuts at: 1318 1318
Size: 4280
Acc65I G'GTAC_C
Cuts at: 649 1296 649
Size: 647 3633
AclI AA'CG_TT
Cuts at: 4120 4120
Size: 4280
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 2105 2537 2890 3552 178
Size: 1927 432 353 662 906
Fragments arranged by size:
1927 906 662 432 353
AflIII A'CryG_T
Cuts at: 1632 3432 3713 1632
Size: 1800 281 2199
Fragments arranged by size:
2199 1800 281
AleI CACnn'nnGTG
Cuts at: 1201 1201
Size: 4280
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 989 989
Size: 4280
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 121 153 121
Size: 32 4248
AlwNI CAG_nnn'CTG
Cuts at: 447 530 1585 3023 447
Size: 83 1055 1438 1704
Fragments arranged by size:
1704 1438 1055 83
ApaI G_GGCC'C
Cuts at: 468 1086 468
Size: 618 3662
ApaLI G'TGCA_C
Cuts at: 3118 3118
Size: 4280
ApoI r'AATT_y
Cuts at: 1284 3633 3889 1284
Size: 2349 256 1675
Fragments arranged by size:
2349 1675 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4280
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4280
AvaI C'yCGr_G
Cuts at: 461 467 548 1022 1300 461
Size: 6 81 474 278 3441
Fragments arranged by size:
3441 474 278 81 6
BamHI G'GATC_C
Cuts at: 416 1115 1305 416
Size: 699 190 3391
Fragments arranged by size:
3391 699 190
BanI G'GyrC_C
Cuts at: 649 1000 1018 1296 1710 1958 1993 649
Size: 351 18 278 414 248 35 2936
Fragments arranged by size:
2936 414 351 278 248 35 18
BanII G_rGCy'C
Cuts at: 305 468 728 1086 1165 1294 2324 305
Size: 163 260 358 79 129 1030 2261
Fragments arranged by size:
2261 1030 358 260 163 129 79
BbeI G_GCGC'C
Cuts at: 1714 1962 1714
Size: 248 4032
BbsI GAAGACnn'nnnn_
Cuts at: 870 870
Size: 4280
BbvCI CC'TCA_GC
Cuts at: 914 914
Size: 4280
BciVI GTATCCnnnnn_n'
Cuts at: 2178 3234 4280 2178
Size: 1056 1046 2178
Fragments arranged by size:
2178 1056 1046
BclI T'GATC_A
Cuts at: 1800 1800
Size: 4280
BglII A'GATC_T
Cuts at: 1795 3832 1795
Size: 2037 2243
BlpI GC'TnA_GC
Cuts at: 909 1392 909
Size: 483 3797
Bme1580I G_kGCm'C
Cuts at: 468 1005 1086 1905 1998 3122 468
Size: 537 81 819 93 1124 1626
Fragments arranged by size:
1626 1124 819 537 93 81
BmgBI CAC'GTC
Cuts at: 1203 1203
Size: 4280
BmrI ACTGGGnnnn_n'
Cuts at: 1492 1573 1908 4052 1492
Size: 81 335 2144 1720
Fragments arranged by size:
2144 1720 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 799 2647 799
Size: 1848 2432
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1003 1179 1201 1003
Size: 176 22 4082
Fragments arranged by size:
4082 176 22
Bpu10I CC'TnA_GC
Cuts at: 914 1038 914
Size: 124 4156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 769 1456 2335 3049 3347 769
Size: 687 879 714 298 1702
Fragments arranged by size:
1702 879 714 687 298
BsaI GGTCTCn'nnnn_
Cuts at: 557 3984 557
Size: 3427 853
BsaAI yAC'GTr
Cuts at: 1633 1633
Size: 4280
BsaBI GATnn'nnATC
Cuts at: 6 1794 3831 6
Size: 1788 2037 455
Fragments arranged by size:
2037 1788 455
BsaHI Gr'CG_yC
Cuts at: 1711 1959 2661 4157 1711
Size: 248 702 1496 1834
Fragments arranged by size:
1834 1496 702 248
BsaWI w'CCGG_w
Cuts at: 237 413 1469 1990 2668 3079 3226 3500 237
Size: 176 1056 521 678 411 147 274 1017
Fragments arranged by size:
1056 1017 678 521 411 274 176 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 121 151 436 466 1138 1168 121
Size: 30 285 30 672 30 3233
Fragments arranged by size:
3233 672 285 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 337 337
Size: 4280
BseYI C'CCAG_C
Cuts at: 673 760 1048 1705 2463 3128 3536 673
Size: 87 288 657 758 665 408 1417
Fragments arranged by size:
1417 758 665 657 408 288 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 904 904
Size: 4280
BsiEI CG_ry'CG
Cuts at: 104 146 1822 1868 3098 104
Size: 42 1676 46 1230 1286
Fragments arranged by size:
1676 1286 1230 46 42
BsiHKAI G_wGCw'C
Cuts at: 305 728 1294 2072 2262 3122 305
Size: 423 566 778 190 860 1463
Fragments arranged by size:
1463 860 778 566 423 190
Bsp1286I G_dGCh'C
Cuts at: 305 468 728 1005 1086 1165 1294 1905 1998
Size: 163 260 277 81 79 129 611 93
Cuts at: 1998 2072 2262 2324 3122 305
Size: 74 190 62 798 1463
Fragments arranged by size:
1463 798 611 277 260 190 163 129
93 81 79 74 62
BspEI T'CCGG_A
Cuts at: 413 413
Size: 4280
BspHI T'CATG_A
Cuts at: 2712 2712
Size: 4280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 538 1330 1544 1846 2227 538
Size: 792 214 302 381 2591
Fragments arranged by size:
2591 792 381 302 214
BsrBI CCG'CTC
Cuts at: 50 132 434 585 621 645 2572 2626 4110
Size: 82 302 151 36 24 1927 54 1484
Cuts at: 4110 4275 50
Size: 165 55
Fragments arranged by size:
1927 1484 302 165 151 82 55 54
36 24
BsrDI GCAATG_nn'
Cuts at: 2192 2192
Size: 4280
BsrFI r'CCGG_y
Cuts at: 766 835 2278 2459 766
Size: 69 1443 181 2587
Fragments arranged by size:
2587 1443 181 69
BssSI C'ACGA_G
Cuts at: 193 2551 3259 193
Size: 2358 708 1214
Fragments arranged by size:
2358 1214 708
BstAPI GCAn_nnn'nTGC
Cuts at: 530 1657 530
Size: 1127 3153
BstBI TT'CG_AA
Cuts at: 390 480 2641 390
Size: 90 2161 2029
Fragments arranged by size:
2161 2029 90
BstXI CCAn_nnnn'nTGG
Cuts at: 660 1056 1134 660
Size: 396 78 3806
Fragments arranged by size:
3806 396 78
BstYI r'GATC_y
Cuts at: 1 416 1008 1115 1305 1772 1795 2130 2376
Size: 415 592 107 190 467 23 335 246
Cuts at: 2376 2682 2694 2780 3832 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 592 467 449 415 335 306 246
190 107 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 750 750
Size: 4280
BtgI C'CryG_G
Cuts at: 107 920 107
Size: 813 3467
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1092 2230 2411 2574 1092
Size: 1138 181 163 2798
Fragments arranged by size:
2798 1138 181 163
BtsI GCAGTG_nn'
Cuts at: 892 1523 892
Size: 631 3649
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 303 338 303
Size: 35 4245
DraI TTT'AAA
Cuts at: 1263 1279 1263
Size: 16 4264
DraIII CAC_nnn'GTG
Cuts at: 846 846
Size: 4280
DrdI GACnn_nn'nnGTC
Cuts at: 1986 3330 1986
Size: 1344 2936
EaeI y'GGCC_r
Cuts at: 143 429 837 950 1124 1865 2039 2430 2457
Size: 286 408 113 174 741 174 391 27
Cuts at: 2457 3802 143
Size: 1345 621
Fragments arranged by size:
1345 741 621 408 391 286 174 174
113 27
EagI C'GGCC_G
Cuts at: 143 1865 143
Size: 1722 2558
EarI CTCTTCn'nnn_
Cuts at: 1145 2303 2513 1145
Size: 1158 210 2912
Fragments arranged by size:
2912 1158 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2488 3230 3376 4090 2488
Size: 742 146 714 2678
Fragments arranged by size:
2678 742 714 146
EcoICRI GAG'CTC
Cuts at: 303 726 1292 303
Size: 423 566 3291
Fragments arranged by size:
3291 566 423
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 1003 1179 1201 2105 2537 2890 3552 178
Size: 825 176 22 904 432 353 662 906
Fragments arranged by size:
906 904 825 662 432 353 176 22
EcoNI CCTnn'n_nnAGG
Cuts at: 741 1183 741
Size: 442 3838
EcoO109I rG'GnC_Cy
Cuts at: 444 474 543 706 746 1042 1062 1082 1097
Size: 30 69 163 40 296 20 20 15
Cuts at: 1097 1140 1419 444
Size: 43 279 3305
Fragments arranged by size:
3305 296 279 163 69 43 40 30
20 20 15
EcoRI G'AATT_C
Cuts at: 1284 1284
Size: 4280
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2299 2299
Size: 4280
FspI TGC'GCA
Cuts at: 2061 2061
Size: 4280
HaeII r_GCGC'y
Cuts at: 767 1714 1962 3192 3963 767
Size: 947 248 1230 771 1084
Fragments arranged by size:
1230 1084 947 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 436 468 1138 1170 1192 1224 2122 2152 2154
Size: 32 670 32 22 32 898 30 2
Cuts at: 2154 2184 436
Size: 30 2532
Fragments arranged by size:
2532 898 670 32 32 32 30 30
22 2
HincII GTy'rAC
Cuts at: 1319 1319
Size: 4280
KasI G'GCGC_C
Cuts at: 1710 1958 1710
Size: 248 4032
KpnI G_GTAC'C
Cuts at: 653 1300 653
Size: 647 3633
MluI A'CGCG_T
Cuts at: 3713 3713
Size: 4280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1053 3039 3223 1053
Size: 1986 184 2110
Fragments arranged by size:
2110 1986 184
MscI TGG'CCA
Cuts at: 1126 2041 1126
Size: 915 3365
MslI CAynn'nnrTG
Cuts at: 1201 2269 2396 1201
Size: 1068 127 3085
Fragments arranged by size:
3085 1068 127
MspA1I CmG'CkG
Cuts at: 121 855 913 1391 1705 2065 2849 3094 121
Size: 734 58 478 314 360 784 245 1307
Fragments arranged by size:
1307 784 734 478 360 314 245 58
NaeI GCC'GGC
Cuts at: 768 837 2461 768
Size: 69 1624 2587
Fragments arranged by size:
2587 1624 69
NarI GG'CG_CC
Cuts at: 1711 1959 1711
Size: 248 4032
NcoI C'CATG_G
Cuts at: 107 107
Size: 4280
NgoMIV G'CCGG_C
Cuts at: 766 835 2459 766
Size: 69 1624 2587
Fragments arranged by size:
2587 1624 69
NspI r_CATG'y
Cuts at: 1333 1833 3436 1333
Size: 500 1603 2177
Fragments arranged by size:
2177 1603 500
PasI CC'CwG_GG
Cuts at: 1086 1086
Size: 4280
PciI A'CATG_T
Cuts at: 3432 3432
Size: 4280
PflMI CCAn_nnn'nTGG
Cuts at: 447 447
Size: 4280
PfoI T'CCnGG_A
Cuts at: 251 728 980 1156 251
Size: 477 252 176 3375
Fragments arranged by size:
3375 477 252 176
PmeI GTTT'AAAC
Cuts at: 1279 1279
Size: 4280
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 121 153 2726 2758 121
Size: 32 2573 32 1643
Fragments arranged by size:
2573 1643 32 32
PpuMI rG'GwC_Cy
Cuts at: 444 1140 444
Size: 696 3584
PspOMI G'GGCC_C
Cuts at: 464 1082 464
Size: 618 3662
PstI C_TGCA'G
Cuts at: 1327 2012 1327
Size: 685 3595
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4280
PvuII CAG'CTG
Cuts at: 855 913 1705 2065 855
Size: 58 792 360 3070
Fragments arranged by size:
3070 792 360 58
RsrII CG'GwC_CG
Cuts at: 2475 2475
Size: 4280
SacI G_AGCT'C
Cuts at: 305 728 1294 305
Size: 423 566 3291
Fragments arranged by size:
3291 566 423
SalI G'TCGA_C
Cuts at: 1317 1317
Size: 4280
SanDI GG'GwC_CC
Cuts at: 1140 1140
Size: 4280
SapI GCTCTTCn'nnn_
Cuts at: 2303 2513 2303
Size: 210 4070
SbfI CC_TGCA'GG
Cuts at: 1327 1327
Size: 4280
ScaI AGT'ACT
Cuts at: 3797 3797
Size: 4280
SexAI A'CCwGG_T
Cuts at: 878 878
Size: 4280
SfcI C'TryA_G
Cuts at: 33 1323 2008 2976 3167 33
Size: 1290 685 968 191 1146
Fragments arranged by size:
1290 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1712 1960 1712
Size: 248 4032
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4280
SmaI CCC'GGG
Cuts at: 463 469 1024 1302 463
Size: 6 555 278 3441
Fragments arranged by size:
3441 555 278 6
SmlI C'TyrA_G
Cuts at: 784 1435 2350 3064 3326 784
Size: 651 915 714 262 1738
Fragments arranged by size:
1738 915 714 651 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4280
SphI G_CATG'C
Cuts at: 1333 1833 1333
Size: 500 3780
StyI C'CwwG_G
Cuts at: 107 362 1251 1414 1768 107
Size: 255 889 163 354 2619
Fragments arranged by size:
2619 889 354 255 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 575 1863 1996 2455 2664 575
Size: 1288 133 459 209 2191
Fragments arranged by size:
2191 1288 459 209 133
TatI w'GTAC_w
Cuts at: 221 3795 221
Size: 3574 706
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3797 3829 3797
Size: 32 4248
Tth111I GACn'n_nGTC
Cuts at: 2077 2077
Size: 4280
XbaI T'CTAG_A
Cuts at: 1311 1311
Size: 4280
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 927 1119 1775 927
Size: 192 656 3432
Fragments arranged by size:
3432 656 192
XmaI C'CCGG_G
Cuts at: 461 467 1022 1300 461
Size: 6 555 278 3441
Fragments arranged by size:
3441 555 278 6
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AscI AvrII BaeI
BcgI BfrBI BglI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I ClaI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PmlI PshAI PsiI PspXI PsrI SacII SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI