Restriction map for
pF1KB0039
(Circular) (Six-base) MAPSORT of: pF1KB0039.seq Check: 283 from: 1 to: 4649
pF1KB0039 4649 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1415 1415
Size: 4649
AccI GT'mk_AC
Cuts at: 1687 1687
Size: 4649
Acc65I G'GTAC_C
Cuts at: 1024 1665 1024
Size: 641 4008
AclI AA'CG_TT
Cuts at: 1617 4489 1617
Size: 2872 1777
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 874 2474 2906 3259 3921 874
Size: 1600 432 353 662 1602
Fragments arranged by size:
1602 1600 662 432 353
AflIII A'CryG_T
Cuts at: 2001 3801 4082 2001
Size: 1800 281 2568
Fragments arranged by size:
2568 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1310 1310
Size: 4649
AlwNI CAG_nnn'CTG
Cuts at: 820 1434 1954 3392 820
Size: 614 520 1438 2077
Fragments arranged by size:
2077 1438 614 520
ApaLI G'TGCA_C
Cuts at: 540 3487 540
Size: 2947 1702
ApoI r'AATT_y
Cuts at: 165 736 972 1653 4002 4258 165
Size: 571 236 681 2349 256 556
Fragments arranged by size:
2349 681 571 556 256 236
AseI AT'TA_AT
Cuts at: 20 20
Size: 4649
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4649
AvaI C'yCGr_G
Cuts at: 1275 1669 1275
Size: 394 4255
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1263 1296 1263
Size: 33 4616
BamHI G'GATC_C
Cuts at: 1674 1674
Size: 4649
BanI G'GyrC_C
Cuts at: 196 1024 1237 1665 2079 2327 2362 196
Size: 828 213 428 414 248 35 2483
Fragments arranged by size:
2483 828 428 414 248 213 35
BanII G_rGCy'C
Cuts at: 1137 1663 2693 1137
Size: 526 1030 3093
Fragments arranged by size:
3093 1030 526
BbeI G_GCGC'C
Cuts at: 2083 2331 2083
Size: 248 4401
BbsI GAAGACnn'nnnn_
Cuts at: 509 509
Size: 4649
BbvCI CC'TCA_GC
Cuts at: 1057 1057
Size: 4649
BciVI GTATCCnnnnn_n'
Cuts at: 1291 2547 3603 4649 1291
Size: 1256 1056 1046 1291
Fragments arranged by size:
1291 1256 1056 1046
BclI T'GATC_A
Cuts at: 1425 2169 1425
Size: 744 3905
BfrBI ATG'CAT
Cuts at: 453 453
Size: 4649
BglII A'GATC_T
Cuts at: 222 2164 4201 222
Size: 1942 2037 670
Fragments arranged by size:
2037 1942 670
BlpI GC'TnA_GC
Cuts at: 1761 1761
Size: 4649
Bme1580I G_kGCm'C
Cuts at: 544 2274 2367 3491 544
Size: 1730 93 1124 1702
Fragments arranged by size:
1730 1702 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 855 1861 1942 2277 4421 855
Size: 1006 81 335 2144 1083
Fragments arranged by size:
2144 1083 1006 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 732 3016 732
Size: 2284 2365
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 1172 772
Size: 400 4249
Bpu10I CC'TnA_GC
Cuts at: 622 925 1057 1504 622
Size: 303 132 447 3767
Fragments arranged by size:
3767 447 303 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1825 2704 3418 3716 1825
Size: 879 714 298 2758
Fragments arranged by size:
2758 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4353 4353
Size: 4649
BsaAI yAC'GTr
Cuts at: 2002 2002
Size: 4649
BsaBI GATnn'nnATC
Cuts at: 6 2163 4200 6
Size: 2157 2037 455
Fragments arranged by size:
2157 2037 455
BsaHI Gr'CG_yC
Cuts at: 2080 2328 3030 4526 2080
Size: 248 702 1496 2203
Fragments arranged by size:
2203 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1838 2359 3037 3448 3595 3869 1838
Size: 521 678 411 147 274 2618
Fragments arranged by size:
2618 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 894 894
Size: 4649
BseYI C'CCAG_C
Cuts at: 779 2074 2832 3497 3905 779
Size: 1295 758 665 408 1523
Fragments arranged by size:
1523 1295 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 965 1022 1627 965
Size: 57 605 3987
Fragments arranged by size:
3987 605 57
BsiEI CG_ry'CG
Cuts at: 104 2191 2237 3467 104
Size: 2087 46 1230 1286
Fragments arranged by size:
2087 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 544 1067 1249 1663 2441 2631 3491 544
Size: 523 182 414 778 190 860 1702
Fragments arranged by size:
1702 860 778 523 414 190 182
BsmI GAATG_Cn'
Cuts at: 423 598 960 423
Size: 175 362 4112
Fragments arranged by size:
4112 362 175
BsmBI CGTCTCn'nnnn_
Cuts at: 183 183
Size: 4649
Bsp1286I G_dGCh'C
Cuts at: 544 1067 1137 1249 1663 2274 2367 2441 2631
Size: 523 70 112 414 611 93 74 190
Cuts at: 2631 2693 3491 544
Size: 62 798 1702
Fragments arranged by size:
1702 798 611 523 414 190 112 93
74 70 62
BspHI T'CATG_A
Cuts at: 3081 3081
Size: 4649
BspMI ACCTGCnnnn'nnnn_
Cuts at: 483 1415 1442 1699 1913 2215 2596 483
Size: 932 27 257 214 302 381 2536
Fragments arranged by size:
2536 932 381 302 257 214 27
BsrBI CCG'CTC
Cuts at: 50 2941 2995 4479 4644 50
Size: 2891 54 1484 165 55
Fragments arranged by size:
2891 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 153 692 965 2561 153
Size: 539 273 1596 2241
Fragments arranged by size:
2241 1596 539 273
BsrFI r'CCGG_y
Cuts at: 2647 2828 2647
Size: 181 4468
BsrGI T'GTAC_A
Cuts at: 1316 1316
Size: 4649
BssSI C'ACGA_G
Cuts at: 2920 3628 2920
Size: 708 3941
BstAPI GCAn_nnn'nTGC
Cuts at: 368 798 988 1000 2026 368
Size: 430 190 12 1026 2991
Fragments arranged by size:
2991 1026 430 190 12
BstBI TT'CG_AA
Cuts at: 296 1621 3010 296
Size: 1325 1389 1935
Fragments arranged by size:
1935 1389 1325
BstXI CCAn_nnnn'nTGG
Cuts at: 266 1420 266
Size: 1154 3495
BstYI r'GATC_y
Cuts at: 1 222 1674 2141 2164 2499 2745 3051 3063
Size: 221 1452 467 23 335 246 306 12
Cuts at: 3063 3149 4201 1
Size: 86 1052 449
Fragments arranged by size:
1452 1052 467 449 335 306 246 221
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 331 331
Size: 4649
BtgI C'CryG_G
Cuts at: 107 662 704 725 1250 107
Size: 555 42 21 525 3506
Fragments arranged by size:
3506 555 525 42 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2599 2780 2943 2599
Size: 181 163 4305
Fragments arranged by size:
4305 181 163
BtsI GCAGTG_nn'
Cuts at: 1227 1892 1227
Size: 665 3984
DraI TTT'AAA
Cuts at: 1202 1648 1202
Size: 446 4203
DraIII CAC_nnn'GTG
Cuts at: 1021 1021
Size: 4649
DrdI GACnn_nn'nnGTC
Cuts at: 559 2355 3699 559
Size: 1796 1344 1509
Fragments arranged by size:
1796 1509 1344
EaeI y'GGCC_r
Cuts at: 2234 2408 2799 2826 4171 2234
Size: 174 391 27 1345 2712
Fragments arranged by size:
2712 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2234 2234
Size: 4649
EarI CTCTTCn'nnn_
Cuts at: 298 428 576 763 2672 2882 298
Size: 130 148 187 1909 210 2065
Fragments arranged by size:
2065 1909 210 187 148 130
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2857 3599 3745 4459 2857
Size: 742 146 714 3047
Fragments arranged by size:
3047 742 714 146
EcoICRI GAG'CTC
Cuts at: 1661 1661
Size: 4649
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 874 1172 2474 2906 3259 3921 772
Size: 102 298 1302 432 353 662 1500
Fragments arranged by size:
1500 1302 662 432 353 298 102
EcoNI CCTnn'n_nnAGG
Cuts at: 1264 1411 1264
Size: 147 4502
EcoO109I rG'GnC_Cy
Cuts at: 263 610 1079 1257 1431 1788 263
Size: 347 469 178 174 357 3124
Fragments arranged by size:
3124 469 357 347 178 174
EcoRI G'AATT_C
Cuts at: 165 1653 165
Size: 1488 3161
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2668 2668
Size: 4649
FspI TGC'GCA
Cuts at: 2430 2430
Size: 4649
HaeII r_GCGC'y
Cuts at: 2083 2331 3561 4332 2083
Size: 248 1230 771 2400
Fragments arranged by size:
2400 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 135 142 167 174 2491 2521 2523 2553 135
Size: 7 25 7 2317 30 2 30 2231
Fragments arranged by size:
2317 2231 30 30 25 7 7 2
HincII GTy'rAC
Cuts at: 1113 1688 1113
Size: 575 4074
KasI G'GCGC_C
Cuts at: 2079 2327 2079
Size: 248 4401
KpnI G_GTAC'C
Cuts at: 1028 1669 1028
Size: 641 4008
MluI A'CGCG_T
Cuts at: 4082 4082
Size: 4649
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 104 3408 3592 104
Size: 3304 184 1161
Fragments arranged by size:
3304 1161 184
MscI TGG'CCA
Cuts at: 2410 2410
Size: 4649
MslI CAynn'nnrTG
Cuts at: 822 2638 2765 822
Size: 1816 127 2706
Fragments arranged by size:
2706 1816 127
MspA1I CmG'CkG
Cuts at: 1760 2074 2434 3218 3463 1760
Size: 314 360 784 245 2946
Fragments arranged by size:
2946 784 360 314 245
NaeI GCC'GGC
Cuts at: 2830 2830
Size: 4649
NarI GG'CG_CC
Cuts at: 2080 2328 2080
Size: 248 4401
NcoI C'CATG_G
Cuts at: 107 662 704 725 1250 107
Size: 555 42 21 525 3506
Fragments arranged by size:
3506 555 525 42 21
NgoMIV G'CCGG_C
Cuts at: 2828 2828
Size: 4649
NsiI A_TGCA'T
Cuts at: 455 455
Size: 4649
NspI r_CATG'y
Cuts at: 1702 2202 3805 1702
Size: 500 1603 2546
Fragments arranged by size:
2546 1603 500
PasI CC'CwG_GG
Cuts at: 445 445
Size: 4649
PciI A'CATG_T
Cuts at: 3801 3801
Size: 4649
PflMI CCAn_nnn'nTGG
Cuts at: 942 942
Size: 4649
PmeI GTTT'AAAC
Cuts at: 1648 1648
Size: 4649
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3095 3127 3095
Size: 32 4617
PpuMI rG'GwC_Cy
Cuts at: 263 610 1079 1431 263
Size: 347 469 352 3481
Fragments arranged by size:
3481 469 352 347
PstI C_TGCA'G
Cuts at: 996 1142 1442 1696 2381 996
Size: 146 300 254 685 3264
Fragments arranged by size:
3264 685 300 254 146
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4649
PvuII CAG'CTG
Cuts at: 2074 2434 2074
Size: 360 4289
RsrII CG'GwC_CG
Cuts at: 2844 2844
Size: 4649
SacI G_AGCT'C
Cuts at: 1663 1663
Size: 4649
SalI G'TCGA_C
Cuts at: 1686 1686
Size: 4649
SapI GCTCTTCn'nnn_
Cuts at: 428 2672 2882 428
Size: 2244 210 2195
Fragments arranged by size:
2244 2195 210
SbfI CC_TGCA'GG
Cuts at: 1696 1696
Size: 4649
ScaI AGT'ACT
Cuts at: 4166 4166
Size: 4649
SexAI A'CCwGG_T
Cuts at: 265 265
Size: 4649
SfcI C'TryA_G
Cuts at: 33 992 1138 1438 1692 2377 3345 3536 33
Size: 959 146 300 254 685 968 191 1146
Fragments arranged by size:
1146 968 959 685 300 254 191 146
SfoI GGC'GCC
Cuts at: 2081 2329 2081
Size: 248 4401
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4649
SmaI CCC'GGG
Cuts at: 1671 1671
Size: 4649
SmlI C'TyrA_G
Cuts at: 1804 2719 3433 3695 1804
Size: 915 714 262 2758
Fragments arranged by size:
2758 915 714 262
SpeI A'CTAG_T
Cuts at: 67 1069 67
Size: 1002 3647
SphI G_CATG'C
Cuts at: 1702 2202 1702
Size: 500 4149
StuI AGG'CCT
Cuts at: 1270 1270
Size: 4649
StyI C'CwwG_G
Cuts at: 107 259 662 704 725 1250 1475 1783 2137
Size: 152 403 42 21 525 225 308 354
Cuts at: 2137 107
Size: 2619
Fragments arranged by size:
2619 525 403 354 308 225 152 42
21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 224 738 953 2232 2365 2824 3033 224
Size: 514 215 1279 133 459 209 1840
Fragments arranged by size:
1840 1279 514 459 215 209 133
TatI w'GTAC_w
Cuts at: 1316 4164 1316
Size: 2848 1801
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4166 4198 4166
Size: 32 4617
Tth111I GACn'n_nGTC
Cuts at: 267 2446 267
Size: 2179 2470
XbaI T'CTAG_A
Cuts at: 1680 1680
Size: 4649
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2144 2144
Size: 4649
XmaI C'CCGG_G
Cuts at: 1669 1669
Size: 4649
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AloI ApaI
AscI AvrII BcgI BglI BmgBI BmtI BsaXI BsiWI
BspEI BssHII BstEII BstZ17I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI