Restriction map for
pF1KB0044
(Circular) (Six-base) MAPSORT of: pF1KB0044.seq Check: 8023 from: 1 to: 6887
pF1KB0044 6887 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 3925 3925
Size: 6887
Acc65I G'GTAC_C
Cuts at: 1744 3903 1744
Size: 2159 4728
AclI AA'CG_TT
Cuts at: 6727 6727
Size: 6887
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 101 584 904 1171 2533 4712 5144 5891 6159
Size: 483 320 267 1362 2179 432 747 268
Cuts at: 6159 101
Size: 829
Fragments arranged by size:
2179 1362 829 747 483 432 320 268
267
AflIII A'CryG_T
Cuts at: 1986 4239 5343 6320 1986
Size: 2253 1104 977 2553
Fragments arranged by size:
2553 2253 1104 977
AgeI A'CCGG_T
Cuts at: 5275 5275
Size: 6887
AleI CACnn'nnGTG
Cuts at: 2322 2322
Size: 6887
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 952 1774 1964 2780 952
Size: 822 190 816 5059
Fragments arranged by size:
5059 822 816 190
AlwNI CAG_nnn'CTG
Cuts at: 2445 2575 4192 5759 2445
Size: 130 1617 1567 3573
Fragments arranged by size:
3573 1617 1567 130
ApaLI G'TGCA_C
Cuts at: 5657 5657
Size: 6887
ApoI r'AATT_y
Cuts at: 568 625 646 2855 3133 3190 3255 3751 3891
Size: 57 21 2209 278 57 65 496 140
Cuts at: 3891 6240 6496 568
Size: 2349 256 959
Fragments arranged by size:
2349 2209 959 496 278 256 140 65
57 57 21
AseI AT'TA_AT
Cuts at: 20 2524 20
Size: 2504 4383
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6887
AvaI C'yCGr_G
Cuts at: 3907 3907
Size: 6887
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3076 3109 3076
Size: 33 6854
BamHI G'GATC_C
Cuts at: 3912 3912
Size: 6887
BanI G'GyrC_C
Cuts at: 1744 3903 4317 4565 4600 1744
Size: 2159 414 248 35 4031
Fragments arranged by size:
4031 2159 414 248 35
BanII G_rGCy'C
Cuts at: 1582 3695 3901 4931 1582
Size: 2113 206 1030 3538
Fragments arranged by size:
3538 2113 1030 206
BbeI G_GCGC'C
Cuts at: 4321 4569 4321
Size: 248 6639
BbsI GAAGACnn'nnnn_
Cuts at: 1346 2135 2275 3602 1346
Size: 789 140 1327 4631
Fragments arranged by size:
4631 1327 789 140
BbvCI CC'TCA_GC
Cuts at: 1718 1718
Size: 6887
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3646 3680 3646
Size: 34 6853
BciVI GTATCCnnnnn_n'
Cuts at: 3104 4785 5546 6887 3104
Size: 1681 761 1341 3104
Fragments arranged by size:
3104 1681 1341 761
BclI T'GATC_A
Cuts at: 4407 4407
Size: 6887
BfrBI ATG'CAT
Cuts at: 2757 2757
Size: 6887
BglII A'GATC_T
Cuts at: 3566 4402 6439 3566
Size: 836 2037 4014
Fragments arranged by size:
4014 2037 836
BlpI GC'TnA_GC
Cuts at: 3689 3809 3999 3689
Size: 120 190 6577
Fragments arranged by size:
6577 190 120
Bme1580I G_kGCm'C
Cuts at: 4512 4605 5661 4512
Size: 93 1056 5738
Fragments arranged by size:
5738 1056 93
BmgBI CAC'GTC
Cuts at: 2977 2977
Size: 6887
BmrI ACTGGGnnnn_n'
Cuts at: 394 1611 3278 4099 4180 4515 6659 394
Size: 1217 1667 821 81 335 2144 622
Fragments arranged by size:
2144 1667 1217 821 622 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5254 5254
Size: 6887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 1421 1715 2447 3668 251
Size: 1170 294 732 1221 3470
Fragments arranged by size:
3470 1221 1170 732 294
Bpu10I CC'TnA_GC
Cuts at: 1718 3814 1718
Size: 2096 4791
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1568 4063 4942 5434 5732 1568
Size: 2495 879 492 298 2723
Fragments arranged by size:
2723 2495 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3491 6591 3491
Size: 3100 3787
BsaAI yAC'GTr
Cuts at: 1989 4240 1989
Size: 2251 4636
BsaBI GATnn'nnATC
Cuts at: 6 3565 4401 6438 6
Size: 3559 836 2037 455
Fragments arranged by size:
3559 2037 836 455
BsaHI Gr'CG_yC
Cuts at: 4318 4566 5268 6764 4318
Size: 248 702 1496 4441
Fragments arranged by size:
4441 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4076 4597 5275 5549 5696 6107 4076
Size: 521 678 274 147 411 4856
Fragments arranged by size:
4856 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 317 1482 2584 2650 317
Size: 1165 1102 66 4554
Fragments arranged by size:
4554 1165 1102 66
BseYI C'CCAG_C
Cuts at: 4312 5070 5647 6143 4312
Size: 758 577 496 5056
Fragments arranged by size:
5056 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 4429 4475 5683 104
Size: 4325 46 1208 1308
Fragments arranged by size:
4325 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1582 1680 3901 4679 4869 5661 1582
Size: 98 2221 778 190 792 2808
Fragments arranged by size:
2808 2221 792 778 190 98
BsmI GAATG_Cn'
Cuts at: 543 3875 543
Size: 3332 3555
Bsp1286I G_dGCh'C
Cuts at: 1582 1680 3695 3901 4512 4605 4679 4869 4931
Size: 98 2015 206 611 93 74 190 62
Cuts at: 4931 5661 1582
Size: 730 2808
Fragments arranged by size:
2808 2015 730 611 206 190 98 93
74 62
BspEI T'CCGG_A
Cuts at: 6107 6107
Size: 6887
BspHI T'CATG_A
Cuts at: 794 1125 6063 794
Size: 331 4938 1618
Fragments arranged by size:
4938 1618 331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3937 4151 4453 4834 3937
Size: 214 302 381 5990
Fragments arranged by size:
5990 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5179 5233 6717 6882 50
Size: 5129 54 1484 165 55
Fragments arranged by size:
5129 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 404 1661 4799 404
Size: 1257 3138 2492
Fragments arranged by size:
3138 2492 1257
BsrFI r'CCGG_y
Cuts at: 230 1747 4885 5066 5275 230
Size: 1517 3138 181 209 1842
Fragments arranged by size:
3138 1842 1517 209 181
BsrGI T'GTAC_A
Cuts at: 1699 1699
Size: 6887
BssSI C'ACGA_G
Cuts at: 3017 5158 5516 3017
Size: 2141 358 4388
Fragments arranged by size:
4388 2141 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2319 4264 2319
Size: 1945 4942
BstBI TT'CG_AA
Cuts at: 525 5248 525
Size: 4723 2164
BstXI CCAn_nnnn'nTGG
Cuts at: 2956 2956
Size: 6887
BstYI r'GATC_y
Cuts at: 1 2238 3566 3912 4379 4402 4737 4983 5995
Size: 2237 1328 346 467 23 335 246 1012
Cuts at: 5995 6081 6093 6439 1
Size: 86 12 346 449
Fragments arranged by size:
2237 1328 1012 467 449 346 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 312 407 3491 3764 312
Size: 95 3084 273 3435
Fragments arranged by size:
3435 3084 273 95
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 435 1363 4837 5018 5181 435
Size: 928 3474 181 163 2141
Fragments arranged by size:
3474 2141 928 181 163
BtsI GCAGTG_nn'
Cuts at: 2046 2317 2329 2446 4130 2046
Size: 271 12 117 1684 4803
Fragments arranged by size:
4803 1684 271 117 12
ClaI AT'CG_AT
Cuts at: 2109 2109
Size: 6887
DraI TTT'AAA
Cuts at: 3066 3886 3066
Size: 820 6067
DraIII CAC_nnn'GTG
Cuts at: 1365 1365
Size: 6887
DrdI GACnn_nn'nnGTC
Cuts at: 735 748 4593 5451 735
Size: 13 3845 858 2171
Fragments arranged by size:
3845 2171 858 13
EaeI y'GGCC_r
Cuts at: 228 4472 4646 5037 5064 6409 228
Size: 4244 174 391 27 1345 706
Fragments arranged by size:
4244 1345 706 391 174 27
EagI C'GGCC_G
Cuts at: 4472 4472
Size: 6887
EarI CTCTTCn'nnn_
Cuts at: 271 312 883 1127 4910 5120 271
Size: 41 571 244 3783 210 2038
Fragments arranged by size:
3783 2038 571 244 210 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5095 5405 5551 6697 5095
Size: 310 146 1146 5285
Fragments arranged by size:
5285 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1580 3899 1580
Size: 2319 4568
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 101 251 584 904 1171 1421 1715 2447 2533
Size: 150 333 320 267 250 294 732 86
Cuts at: 2533 3668 4712 5144 5891 6159 101
Size: 1135 1044 432 747 268 829
Fragments arranged by size:
1135 1044 829 747 732 432 333 320
294 268 267 250 150 86
EcoNI CCTnn'n_nnAGG
Cuts at: 1640 1640
Size: 6887
EcoO109I rG'GnC_Cy
Cuts at: 2544 2923 4026 2544
Size: 379 1103 5405
Fragments arranged by size:
5405 1103 379
EcoRI G'AATT_C
Cuts at: 3891 3891
Size: 6887
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1345 4906 1345
Size: 3561 3326
FseI GG_CCGG'CC
Cuts at: 234 234
Size: 6887
FspI TGC'GCA
Cuts at: 4668 4668
Size: 6887
HaeII r_GCGC'y
Cuts at: 4321 4569 5591 6570 4321
Size: 248 1022 979 4638
Fragments arranged by size:
4638 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2926 2958 3274 3306 3414 3446 4729 4759 4761
Size: 32 316 32 108 32 1283 30 2
Cuts at: 4761 4791 2926
Size: 30 5022
Fragments arranged by size:
5022 1283 316 108 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2070 2201 2751 3926 2070
Size: 131 550 1175 5031
Fragments arranged by size:
5031 1175 550 131
HpaI GTT'AAC
Cuts at: 2201 2201
Size: 6887
KasI G'GCGC_C
Cuts at: 4317 4565 4317
Size: 248 6639
KpnI G_GTAC'C
Cuts at: 1748 3907 1748
Size: 2159 4728
MluI A'CGCG_T
Cuts at: 6320 6320
Size: 6887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 887 5558 5742 887
Size: 4671 184 2032
Fragments arranged by size:
4671 2032 184
MscI TGG'CCA
Cuts at: 4648 4648
Size: 6887
MslI CAynn'nnrTG
Cuts at: 167 1316 1504 1544 1946 2322 2954 3044 3637
Size: 1149 188 40 402 376 632 90 593
Cuts at: 3637 4876 5003 167
Size: 1239 127 2051
Fragments arranged by size:
2051 1239 1149 632 593 402 376 188
127 90 40
MspA1I CmG'CkG
Cuts at: 1399 2088 2764 3998 4312 4672 5685 5930 1399
Size: 689 676 1234 314 360 1013 245 2356
Fragments arranged by size:
2356 1234 1013 689 676 360 314 245
NaeI GCC'GGC
Cuts at: 232 5068 232
Size: 4836 2051
NarI GG'CG_CC
Cuts at: 4318 4566 4318
Size: 248 6639
NcoI C'CATG_G
Cuts at: 3491 3764 3491
Size: 273 6614
NgoMIV G'CCGG_C
Cuts at: 230 5066 230
Size: 4836 2051
NsiI A_TGCA'T
Cuts at: 2759 2759
Size: 6887
NspI r_CATG'y
Cuts at: 1549 2757 2761 3661 3940 4440 5347 1549
Size: 1208 4 900 279 500 907 3089
Fragments arranged by size:
3089 1208 907 900 500 279 4
PciI A'CATG_T
Cuts at: 5343 5343
Size: 6887
PflMI CCAn_nnn'nTGG
Cuts at: 2575 2649 2575
Size: 74 6813
PmeI GTTT'AAAC
Cuts at: 3886 3886
Size: 6887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6026 6058 6026
Size: 32 6855
PpuMI rG'GwC_Cy
Cuts at: 2923 2923
Size: 6887
PstI C_TGCA'G
Cuts at: 1172 1860 2017 2324 2769 3840 3934 4619 1172
Size: 688 157 307 445 1071 94 685 3440
Fragments arranged by size:
3440 1071 688 685 445 307 157 94
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6887
PvuII CAG'CTG
Cuts at: 1399 2088 2764 4312 4672 1399
Size: 689 676 1548 360 3614
Fragments arranged by size:
3614 1548 689 676 360
RsrII CG'GwC_CG
Cuts at: 5082 5082
Size: 6887
SacI G_AGCT'C
Cuts at: 1582 3901 1582
Size: 2319 4568
SalI G'TCGA_C
Cuts at: 3924 3924
Size: 6887
SapI GCTCTTCn'nnn_
Cuts at: 271 312 4910 5120 271
Size: 41 4598 210 2038
Fragments arranged by size:
4598 2038 210 41
SbfI CC_TGCA'GG
Cuts at: 3934 3934
Size: 6887
ScaI AGT'ACT
Cuts at: 3125 6404 3125
Size: 3279 3608
SfcI C'TryA_G
Cuts at: 33 1168 1856 1917 2013 2025 2256 2320 2765
Size: 1135 688 61 96 12 231 64 445
Cuts at: 2765 3249 3836 3930 4615 5608 5799 33
Size: 484 587 94 685 993 191 1121
Fragments arranged by size:
1135 1121 993 688 685 587 484 445
231 191 96 94 64 61 12
SfoI GGC'GCC
Cuts at: 4319 4567 4319
Size: 248 6639
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6887
SmaI CCC'GGG
Cuts at: 3909 3909
Size: 6887
SmlI C'TyrA_G
Cuts at: 1583 4042 4957 5449 5711 1583
Size: 2459 915 492 262 2759
Fragments arranged by size:
2759 2459 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6887
SphI G_CATG'C
Cuts at: 2761 3661 3940 4440 2761
Size: 900 279 500 5208
Fragments arranged by size:
5208 900 500 279
StuI AGG'CCT
Cuts at: 2689 2689
Size: 6887
StyI C'CwwG_G
Cuts at: 3491 3764 4021 4375 3491
Size: 273 257 354 6003
Fragments arranged by size:
6003 354 273 257
SwaI ATTT'AAAT
Cuts at: 3066 3066
Size: 6887
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4470 4603 5062 5271 4470
Size: 133 459 209 6086
Fragments arranged by size:
6086 459 209 133
TatI w'GTAC_w
Cuts at: 1367 1699 3123 3468 6402 1367
Size: 332 1424 345 2934 1852
Fragments arranged by size:
2934 1852 1424 345 332
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6404 6436 6404
Size: 32 6855
Tth111I GACn'n_nGTC
Cuts at: 671 4684 671
Size: 4013 2874
XbaI T'CTAG_A
Cuts at: 2004 3918 2004
Size: 1914 4973
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 169 903 4382 169
Size: 734 3479 2674
Fragments arranged by size:
3479 2674 734
XmaI C'CCGG_G
Cuts at: 3907 3907
Size: 6887
XmnI GAAnn'nnTTC
Cuts at: 1286 1637 2909 1286
Size: 351 1272 5264
Fragments arranged by size:
5264 1272 351
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BglI BmtI BsaXI BsgI BsiWI BsmBI BssHII
BstEII BstZ17I Bsu36I CspCI EcoRV FspAI HindIII MfeI
NdeI NheI NotI NruI PacI PasI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI XhoI ZraI