Restriction map for
pF1KB0045
(Circular) (Six-base) MAPSORT of: pF1KB0045.seq Check: 4853 from: 1 to: 7412
pF1KB0045 7412 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4260 4260
Size: 7412
AccI GT'mk_AC
Cuts at: 1983 4450 1983
Size: 2467 4945
Acc65I G'GTAC_C
Cuts at: 2326 4428 2326
Size: 2102 5310
AclI AA'CG_TT
Cuts at: 747 7252 747
Size: 6505 907
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2213 3140 4256 5237 5669 6416 6684 2213
Size: 927 1116 981 432 747 268 2941
Fragments arranged by size:
2941 1116 981 927 747 432 268
AflIII A'CryG_T
Cuts at: 1978 3172 4764 5868 6845 1978
Size: 1194 1592 1104 977 2545
Fragments arranged by size:
2545 1592 1194 1104 977
AgeI A'CCGG_T
Cuts at: 5800 5800
Size: 7412
AleI CACnn'nnGTG
Cuts at: 726 3097 726
Size: 2371 5041
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1013 1013
Size: 7412
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3553 3585 3553
Size: 32 7380
AlwNI CAG_nnn'CTG
Cuts at: 244 742 919 922 2458 4717 6284 244
Size: 498 177 3 1536 2259 1567 1372
Fragments arranged by size:
2259 1567 1536 1372 498 177 3
ApaLI G'TGCA_C
Cuts at: 998 6182 998
Size: 5184 2228
ApoI r'AATT_y
Cuts at: 516 840 1088 1116 1282 1392 1684 2134 2347
Size: 324 248 28 166 110 292 450 213
Cuts at: 2347 2692 2748 2755 2833 2860 3390 3414 4188
Size: 345 56 7 78 27 530 24 774
Cuts at: 4188 4239 4416 6765 7021 516
Size: 51 177 2349 256 907
Fragments arranged by size:
2349 907 774 530 450 345 324 292
256 248 213 177 166 110 78 56
51 28 27 24 7
AseI AT'TA_AT
Cuts at: 20 20
Size: 7412
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7412
AvaI C'yCGr_G
Cuts at: 114 2533 4278 4432 114
Size: 2419 1745 154 3094
Fragments arranged by size:
3094 2419 1745 154
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3047 3080 3773 3806 3047
Size: 33 693 33 6653
Fragments arranged by size:
6653 693 33 33
BamHI G'GATC_C
Cuts at: 3883 4437 3883
Size: 554 6858
BanI G'GyrC_C
Cuts at: 960 2326 3976 4428 4842 5090 5125 960
Size: 1366 1650 452 414 248 35 3247
Fragments arranged by size:
3247 1650 1366 452 414 248 35
BanII G_rGCy'C
Cuts at: 388 2253 3507 4426 5456 388
Size: 1865 1254 919 1030 2344
Fragments arranged by size:
2344 1865 1254 1030 919
BbeI G_GCGC'C
Cuts at: 4846 5094 4846
Size: 248 7164
BbsI GAAGACnn'nnnn_
Cuts at: 978 1297 2414 978
Size: 319 1117 5976
Fragments arranged by size:
5976 1117 319
BbvCI CC'TCA_GC
Cuts at: 3912 4260 3912
Size: 348 7064
BciVI GTATCCnnnnn_n'
Cuts at: 5310 6071 7412 5310
Size: 761 1341 5310
Fragments arranged by size:
5310 1341 761
BclI T'GATC_A
Cuts at: 3525 4932 3525
Size: 1407 6005
BfrBI ATG'CAT
Cuts at: 2015 2015
Size: 7412
BglI GCCn_nnn'nGGC
Cuts at: 267 267
Size: 7412
BglII A'GATC_T
Cuts at: 4927 6964 4927
Size: 2037 5375
BlpI GC'TnA_GC
Cuts at: 986 1862 4524 986
Size: 876 2662 3874
Fragments arranged by size:
3874 2662 876
Bme1580I G_kGCm'C
Cuts at: 1002 5037 5130 6186 1002
Size: 4035 93 1056 2228
Fragments arranged by size:
4035 2228 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1876 2950 4624 4705 5040 7184 1876
Size: 1074 1674 81 335 2144 2104
Fragments arranged by size:
2144 2104 1674 1074 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 402 3267 5779 402
Size: 2865 2512 2035
Fragments arranged by size:
2865 2512 2035
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1448 1513 1972 2519 4289 1448
Size: 65 459 547 1770 4571
Fragments arranged by size:
4571 1770 547 459 65
Bpu10I CC'TnA_GC
Cuts at: 416 3912 4260 416
Size: 3496 348 3568
Fragments arranged by size:
3568 3496 348
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1607 3731 4588 5467 5959 6257 1607
Size: 2124 857 879 492 298 2762
Fragments arranged by size:
2762 2124 879 857 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 7116 7116
Size: 7412
BsaAI yAC'GTr
Cuts at: 4765 4765
Size: 7412
BsaBI GATnn'nnATC
Cuts at: 6 1144 4011 4926 6963 6
Size: 1138 2867 915 2037 455
Fragments arranged by size:
2867 2037 1138 915 455
BsaHI Gr'CG_yC
Cuts at: 4843 5091 5793 7289 4843
Size: 248 702 1496 4966
Fragments arranged by size:
4966 1496 702 248
BsaWI w'CCGG_w
Cuts at: 155 4601 5122 5800 6074 6221 6632 155
Size: 4446 521 678 274 147 411 935
Fragments arranged by size:
4446 935 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1584 1614 3066 3096 1584
Size: 30 1452 30 5900
Fragments arranged by size:
5900 1452 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 140 394 1100 1112 1510 1513 2670 140
Size: 254 706 12 398 3 1157 4882
Fragments arranged by size:
4882 1157 706 398 254 12 3
BseYI C'CCAG_C
Cuts at: 1954 2543 4265 4837 5595 6172 6668 1954
Size: 589 1722 572 758 577 496 2698
Fragments arranged by size:
2698 1722 758 589 577 572 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1338 2295 2587 3554 4037 1338
Size: 957 292 967 483 4713
Fragments arranged by size:
4713 967 957 483 292
BsiEI CG_ry'CG
Cuts at: 104 4954 5000 6208 104
Size: 4850 46 1208 1308
Fragments arranged by size:
4850 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 388 1002 3507 4426 5204 5394 6186 388
Size: 614 2505 919 778 190 792 1614
Fragments arranged by size:
2505 1614 919 792 778 614 190
BsmI GAATG_Cn'
Cuts at: 221 571 796 221
Size: 350 225 6837
Fragments arranged by size:
6837 350 225
BsmBI CGTCTCn'nnnn_
Cuts at: 1449 1625 1449
Size: 176 7236
Bsp1286I G_dGCh'C
Cuts at: 388 1002 2253 3507 4426 5037 5130 5204 5394
Size: 614 1251 1254 919 611 93 74 190
Cuts at: 5394 5456 6186 388
Size: 62 730 1614
Fragments arranged by size:
1614 1254 1251 919 730 614 611 190
93 74 62
BspEI T'CCGG_A
Cuts at: 155 6632 155
Size: 6477 935
BspHI T'CATG_A
Cuts at: 6588 6588
Size: 7412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2466 3031 4260 4462 4676 4978 5359 2466
Size: 565 1229 202 214 302 381 4519
Fragments arranged by size:
4519 1229 565 381 302 214 202
BsrBI CCG'CTC
Cuts at: 50 5704 5758 7242 7407 50
Size: 5654 54 1484 165 55
Fragments arranged by size:
5654 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 715 5324 715
Size: 4609 2803
BsrFI r'CCGG_y
Cuts at: 5410 5591 5800 5410
Size: 181 209 7022
Fragments arranged by size:
7022 209 181
BssSI C'ACGA_G
Cuts at: 5683 6041 5683
Size: 358 7054
BstAPI GCAn_nnn'nTGC
Cuts at: 919 3922 3940 4789 919
Size: 3003 18 849 3542
Fragments arranged by size:
3542 3003 849 18
BstBI TT'CG_AA
Cuts at: 2665 5773 2665
Size: 3108 4304
BstXI CCAn_nnnn'nTGG
Cuts at: 728 2595 728
Size: 1867 5545
BstYI r'GATC_y
Cuts at: 1 152 2365 3883 4006 4437 4904 4927 5262
Size: 151 2213 1518 123 431 467 23 335
Cuts at: 5262 5508 6520 6606 6618 6964 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2213 1518 1012 467 449 431 346 335
246 151 123 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2331 2331
Size: 7412
BtgI C'CryG_G
Cuts at: 107 107
Size: 7412
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1820 5362 5543 5706 1820
Size: 3542 181 163 3526
Fragments arranged by size:
3542 3526 181 163
BtsI GCAGTG_nn'
Cuts at: 770 1555 1975 3707 4655 770
Size: 785 420 1732 948 3527
Fragments arranged by size:
3527 1732 948 785 420
DraI TTT'AAA
Cuts at: 1257 1683 1949 2139 2637 2754 2832 2859 4411
Size: 426 266 190 498 117 78 27 1552
Cuts at: 4411 1257
Size: 4258
Fragments arranged by size:
4258 1552 498 426 266 190 117 78
27
DrdI GACnn_nn'nnGTC
Cuts at: 5118 5976 5118
Size: 858 6554
EaeI y'GGCC_r
Cuts at: 340 4997 5171 5562 5589 6934 340
Size: 4657 174 391 27 1345 818
Fragments arranged by size:
4657 1345 818 391 174 27
EagI C'GGCC_G
Cuts at: 4997 4997
Size: 7412
EarI CTCTTCn'nnn_
Cuts at: 503 2718 3287 3350 3636 4353 5435 5645 503
Size: 2215 569 63 286 717 1082 210 2270
Fragments arranged by size:
2270 2215 1082 717 569 286 210 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5620 5930 6076 7222 5620
Size: 310 146 1146 5810
Fragments arranged by size:
5810 1146 310 146
EcoICRI GAG'CTC
Cuts at: 386 3505 4424 386
Size: 3119 919 3374
Fragments arranged by size:
3374 3119 919
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1448 1513 1972 2213 2519 3140 4256 4289 5237
Size: 65 459 241 306 621 1116 33 948
Cuts at: 5237 5669 6416 6684 1448
Size: 432 747 268 2176
Fragments arranged by size:
2176 1116 948 747 621 459 432 306
268 241 65 33
EcoNI CCTnn'n_nnAGG
Cuts at: 604 2498 604
Size: 1894 5518
EcoO109I rG'GnC_Cy
Cuts at: 4551 4551
Size: 7412
EcoRI G'AATT_C
Cuts at: 840 1088 1116 1282 3390 3414 4416 840
Size: 248 28 166 2108 24 1002 3836
Fragments arranged by size:
3836 2108 1002 248 166 28 24
EcoRV GAT'ATC
Cuts at: 1146 1146
Size: 7412
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3138 5431 3138
Size: 2293 5119
FspI TGC'GCA
Cuts at: 5193 5193
Size: 7412
HaeII r_GCGC'y
Cuts at: 4846 5094 6116 7095 4846
Size: 248 1022 979 5163
Fragments arranged by size:
5163 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 610 634 642 666 1045 1077 2788 2820 3451
Size: 24 8 24 379 32 1711 32 631
Cuts at: 3451 3483 5254 5284 5286 5316 610
Size: 32 1771 30 2 30 2706
Fragments arranged by size:
2706 1771 1711 631 379 32 32 32
30 30 24 24 8 2
HincII GTy'rAC
Cuts at: 485 3370 4451 485
Size: 2885 1081 3446
Fragments arranged by size:
3446 2885 1081
HpaI GTT'AAC
Cuts at: 485 485
Size: 7412
KasI G'GCGC_C
Cuts at: 4842 5090 4842
Size: 248 7164
KpnI G_GTAC'C
Cuts at: 2330 4432 2330
Size: 2102 5310
MfeI C'AATT_G
Cuts at: 578 578
Size: 7412
MluI A'CGCG_T
Cuts at: 6845 6845
Size: 7412
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 111 509 6083 6267 111
Size: 398 5574 184 1256
Fragments arranged by size:
5574 1256 398 184
MscI TGG'CCA
Cuts at: 342 5173 342
Size: 4831 2581
MslI CAynn'nnrTG
Cuts at: 638 726 1968 3097 5401 5528 638
Size: 88 1242 1129 2304 127 2522
Fragments arranged by size:
2522 2304 1242 1129 127 88
MspA1I CmG'CkG
Cuts at: 308 919 2309 3523 3787 4523 4837 5197 6210
Size: 611 1390 1214 264 736 314 360 1013
Cuts at: 6210 6455 308
Size: 245 1265
Fragments arranged by size:
1390 1265 1214 1013 736 611 360 314
264 245
NaeI GCC'GGC
Cuts at: 5593 5593
Size: 7412
NarI GG'CG_CC
Cuts at: 4843 5091 4843
Size: 248 7164
NcoI C'CATG_G
Cuts at: 107 107
Size: 7412
NgoMIV G'CCGG_C
Cuts at: 5591 5591
Size: 7412
NsiI A_TGCA'T
Cuts at: 2017 2017
Size: 7412
NspI r_CATG'y
Cuts at: 1982 2603 3176 4465 4965 5872 1982
Size: 621 573 1289 500 907 3522
Fragments arranged by size:
3522 1289 907 621 573 500
PciI A'CATG_T
Cuts at: 1978 3172 5868 1978
Size: 1194 2696 3522
Fragments arranged by size:
3522 2696 1194
PflMI CCAn_nnn'nTGG
Cuts at: 3937 3961 4003 3937
Size: 24 42 7346
Fragments arranged by size:
7346 42 24
PfoI T'CCnGG_A
Cuts at: 888 3810 888
Size: 2922 4490
PmeI GTTT'AAAC
Cuts at: 4411 4411
Size: 7412
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6551 6583 6551
Size: 32 7380
PsiI TTA'TAA
Cuts at: 1816 1816
Size: 7412
PstI C_TGCA'G
Cuts at: 1410 3042 3882 4257 4459 5144 1410
Size: 1632 840 375 202 685 3678
Fragments arranged by size:
3678 1632 840 685 375 202
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7412
PvuII CAG'CTG
Cuts at: 308 919 2309 3523 3787 4837 5197 308
Size: 611 1390 1214 264 1050 360 2523
Fragments arranged by size:
2523 1390 1214 1050 611 360 264
RsrII CG'GwC_CG
Cuts at: 5607 5607
Size: 7412
SacI G_AGCT'C
Cuts at: 388 3507 4426 388
Size: 3119 919 3374
Fragments arranged by size:
3374 3119 919
SalI G'TCGA_C
Cuts at: 4449 4449
Size: 7412
SapI GCTCTTCn'nnn_
Cuts at: 3350 5435 5645 3350
Size: 2085 210 5117
Fragments arranged by size:
5117 2085 210
SbfI CC_TGCA'GG
Cuts at: 4459 4459
Size: 7412
ScaI AGT'ACT
Cuts at: 459 1323 1800 3516 6929 459
Size: 864 477 1716 3413 942
Fragments arranged by size:
3413 1716 942 864 477
SfcI C'TryA_G
Cuts at: 33 1406 3038 3518 3878 4253 4455 5140 6133
Size: 1373 1632 480 360 375 202 685 993
Cuts at: 6133 6324 33
Size: 191 1121
Fragments arranged by size:
1632 1373 1121 993 685 480 375 360
202 191
SfoI GGC'GCC
Cuts at: 4844 5092 4844
Size: 248 7164
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7412
SmaI CCC'GGG
Cuts at: 4434 4434
Size: 7412
SmlI C'TyrA_G
Cuts at: 1622 3746 4567 5482 5974 6236 1622
Size: 2124 821 915 492 262 2798
Fragments arranged by size:
2798 2124 915 821 492 262
SpeI A'CTAG_T
Cuts at: 67 454 3511 67
Size: 387 3057 3968
Fragments arranged by size:
3968 3057 387
SphI G_CATG'C
Cuts at: 4465 4965 4465
Size: 500 6912
SspI AAT'ATT
Cuts at: 255 393 1040 255
Size: 138 647 6627
Fragments arranged by size:
6627 647 138
StuI AGG'CCT
Cuts at: 492 492
Size: 7412
StyI C'CwwG_G
Cuts at: 107 3129 4546 4900 107
Size: 3022 1417 354 2619
Fragments arranged by size:
3022 2619 1417 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1623 2058 4995 5128 5587 5796 1623
Size: 435 2937 133 459 209 3239
Fragments arranged by size:
3239 2937 459 435 209 133
TatI w'GTAC_w
Cuts at: 457 1321 1722 1798 3514 3583 6927 457
Size: 864 401 76 1716 69 3344 942
Fragments arranged by size:
3344 1716 942 864 401 76 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1656 1688 2225 2257 3066 3098 6929 6961 1656
Size: 32 537 32 809 32 3831 32 2107
Fragments arranged by size:
3831 2107 809 537 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3968 4074 5209 3968
Size: 106 1135 6171
Fragments arranged by size:
6171 1135 106
XbaI T'CTAG_A
Cuts at: 4443 4443
Size: 7412
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3645 4907 3645
Size: 1262 6150
XmaI C'CCGG_G
Cuts at: 4432 4432
Size: 7412
XmnI GAAnn'nnTTC
Cuts at: 822 822
Size: 7412
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI ApaI AscI AvrII BcgI
BmgBI BmtI BsiWI BsrGI BssHII BstEII BstZ17I ClaI
CspCI DraIII FseI FspAI HindIII NdeI NheI NotI
NruI PacI PasI PmlI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SwaI XhoI ZraI