Restriction map for
pF1KB0047
(Circular) (Six-base) MAPSORT of: pF1KB0047.seq Check: 1421 from: 1 to: 9134
pF1KB0047 9134 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4809 4809
Size: 9134
AccI GT'mk_AC
Cuts at: 5787 6172 5787
Size: 385 8749
Acc65I G'GTAC_C
Cuts at: 6150 6150
Size: 9134
AclI AA'CG_TT
Cuts at: 4778 8974 4778
Size: 4196 4938
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 1175 2746 2933 3269 3553 3757 4028 4156
Size: 733 1571 187 336 284 204 271 128
Cuts at: 4156 4228 4414 5098 5611 5992 6959 7391 8138
Size: 72 186 684 513 381 967 432 747
Cuts at: 8138 8406 442
Size: 268 1170
Fragments arranged by size:
1571 1170 967 747 733 684 513 432
381 336 284 271 268 204 187 186
128 72
AflIII A'CryG_T
Cuts at: 5881 6486 7590 8567 5881
Size: 605 1104 977 6448
Fragments arranged by size:
6448 1104 977 605
AgeI A'CCGG_T
Cuts at: 7522 7522
Size: 9134
AhdI GACnn_n'nnGTC
Cuts at: 1481 2982 1481
Size: 1501 7633
AleI CACnn'nnGTG
Cuts at: 2719 2719
Size: 9134
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2657 2657
Size: 9134
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2435 2467 4763 4795 2435
Size: 32 2296 32 6774
Fragments arranged by size:
6774 2296 32 32
AlwNI CAG_nnn'CTG
Cuts at: 652 1593 2899 3063 3357 3885 4422 4485 5568
Size: 941 1306 164 294 528 537 63 1083
Cuts at: 5568 6439 8006 652
Size: 871 1567 1780
Fragments arranged by size:
1780 1567 1306 1083 941 871 537 528
294 164 63
ApaI G_GGCC'C
Cuts at: 4674 4809 4674
Size: 135 8999
ApaLI G'TGCA_C
Cuts at: 7904 7904
Size: 9134
ApoI r'AATT_y
Cuts at: 868 1529 2368 3180 4114 6138 8487 8743 868
Size: 661 839 812 934 2024 2349 256 1259
Fragments arranged by size:
2349 2024 1259 934 839 812 661 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 9134
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9134
AvaI C'yCGr_G
Cuts at: 127 1316 3169 4667 4675 5002 5117 5611 6154
Size: 1189 1853 1498 8 327 115 494 543
Cuts at: 6154 127
Size: 3107
Fragments arranged by size:
3107 1853 1498 1189 543 494 327 115
8
BamHI G'GATC_C
Cuts at: 132 2271 2321 4211 6159 132
Size: 2139 50 1890 1948 3107
Fragments arranged by size:
3107 2139 1948 1890 50
BanI G'GyrC_C
Cuts at: 6029 6150 6564 6812 6847 6029
Size: 121 414 248 35 8316
Fragments arranged by size:
8316 414 248 121 35
BanII G_rGCy'C
Cuts at: 2906 3096 3443 3824 3992 4674 4756 4809 5003
Size: 190 347 381 168 682 82 53 194
Cuts at: 5003 5307 6065 6148 7178 2906
Size: 304 758 83 1030 4862
Fragments arranged by size:
4862 1030 758 682 381 347 304 194
190 168 83 82 53
BbeI G_GCGC'C
Cuts at: 6033 6568 6816 6033
Size: 535 248 8351
Fragments arranged by size:
8351 535 248
BbsI GAAGACnn'nnnn_
Cuts at: 664 838 2041 2703 3581 4234 4465 4748 4862
Size: 174 1203 662 878 653 231 283 114
Cuts at: 4862 664
Size: 4936
Fragments arranged by size:
4936 1203 878 662 653 283 231 174
114
BbvCI CC'TCA_GC
Cuts at: 5983 5983
Size: 9134
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3381 3415 3381
Size: 34 9100
BciVI GTATCCnnnnn_n'
Cuts at: 4149 7032 7793 9134 4149
Size: 2883 761 1341 4149
Fragments arranged by size:
4149 2883 1341 761
BclI T'GATC_A
Cuts at: 1239 4982 6654 1239
Size: 3743 1672 3719
Fragments arranged by size:
3743 3719 1672
BfrBI ATG'CAT
Cuts at: 599 1691 599
Size: 1092 8042
BglI GCCn_nnn'nGGC
Cuts at: 2611 4690 4918 5659 5935 6023 2611
Size: 2079 228 741 276 88 5722
Fragments arranged by size:
5722 2079 741 276 228 88
BglII A'GATC_T
Cuts at: 6649 8686 6649
Size: 2037 7097
BlpI GC'TnA_GC
Cuts at: 6246 6246
Size: 9134
Bme1580I G_kGCm'C
Cuts at: 4674 4809 6759 6852 7908 4674
Size: 135 1950 93 1056 5900
Fragments arranged by size:
5900 1950 1056 135 93
BmrI ACTGGGnnnn_n'
Cuts at: 4308 6114 6346 6427 6762 8906 4308
Size: 1806 232 81 335 2144 4536
Fragments arranged by size:
4536 2144 1806 335 232 81
BmtI G_CTAG'C
Cuts at: 5521 5521
Size: 9134
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1560 3252 3840 7501 1560
Size: 1692 588 3661 3193
Fragments arranged by size:
3661 3193 1692 588
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1025 2537 2845 3685 3835 3907 4306 4444 4474
Size: 1512 308 840 150 72 399 138 30
Cuts at: 4474 4708 4849 4864 4915 5035 5134 5443 5590
Size: 234 141 15 51 120 99 309 147
Cuts at: 5590 5677 5698 5878 5893 6079 1025
Size: 87 21 180 15 186 4080
Fragments arranged by size:
4080 1512 840 399 309 308 234 186
180 150 147 141 138 120 99 87
72 51 30 21 15 15
Bpu10I CC'TnA_GC
Cuts at: 4021 4121 4201 4411 5950 5983 4021
Size: 100 80 210 1539 33 7172
Fragments arranged by size:
7172 1539 210 100 80 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 3427 3651 3769 4159 5710 6310 7189 7681
Size: 3257 224 118 390 1551 600 879 492
Cuts at: 7681 7979 170
Size: 298 1325
Fragments arranged by size:
3257 1551 1325 879 600 492 390 298
224 118
BsaI GGTCTCn'nnnn_
Cuts at: 1114 1701 4008 4928 8838 1114
Size: 587 2307 920 3910 1410
Fragments arranged by size:
3910 2307 1410 920 587
BsaAI yAC'GTr
Cuts at: 3706 6487 3706
Size: 2781 6353
BsaBI GATnn'nnATC
Cuts at: 6 2326 6648 8685 6
Size: 2320 4322 2037 455
Fragments arranged by size:
4322 2320 2037 455
BsaHI Gr'CG_yC
Cuts at: 5397 6030 6565 6813 7515 9011 5397
Size: 633 535 248 702 1496 5520
Fragments arranged by size:
5520 1496 702 633 535 248
BsaWI w'CCGG_w
Cuts at: 6323 6844 7522 7796 7943 8354 6323
Size: 521 678 274 147 411 7103
Fragments arranged by size:
7103 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 263 293 3895 3925 263
Size: 30 3602 30 5472
Fragments arranged by size:
5472 3602 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2842 3097 3169 3481 3484 3766 3832 4192 4243
Size: 255 72 312 3 282 66 360 51
Cuts at: 4243 4273 4276 4279 4282 4441 4714 4861 5440
Size: 30 3 3 3 159 273 147 579
Cuts at: 5440 5443 5455 5695 2842
Size: 3 12 240 6281
Fragments arranged by size:
6281 579 360 312 282 273 255 240
159 147 72 66 51 30 12 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 652 3692 4838 6559 7317 7894 8390 652
Size: 3040 1146 1721 758 577 496 1396
Fragments arranged by size:
3040 1721 1396 1146 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 6022 6022
Size: 9134
BsiEI CG_ry'CG
Cuts at: 104 1350 3395 5472 6676 6722 7930 104
Size: 1246 2045 2077 1204 46 1208 1308
Fragments arranged by size:
2077 2045 1308 1246 1208 1204 46
BsiHKAI G_wGCw'C
Cuts at: 2906 3443 3824 3956 3992 4756 5003 6065 6148
Size: 537 381 132 36 764 247 1062 83
Cuts at: 6148 6926 7116 7908 2906
Size: 778 190 792 4132
Fragments arranged by size:
4132 1062 792 778 764 537 381 247
190 132 83 36
BsmI GAATG_Cn'
Cuts at: 1756 1756
Size: 9134
BsmBI CGTCTCn'nnnn_
Cuts at: 3382 6100 3382
Size: 2718 6416
Bsp1286I G_dGCh'C
Cuts at: 2906 3096 3443 3824 3956 3992 4674 4756 4809
Size: 190 347 381 132 36 682 82 53
Cuts at: 4809 5003 5307 6065 6148 6759 6852 6926 7116
Size: 194 304 758 83 611 93 74 190
Cuts at: 7116 7178 7908 2906
Size: 62 730 4132
Fragments arranged by size:
4132 758 730 682 611 381 347 304
194 190 190 132 93 83 82 74
62 53 36
BspEI T'CCGG_A
Cuts at: 8354 8354
Size: 9134
BspHI T'CATG_A
Cuts at: 549 2052 4748 8310 549
Size: 1503 2696 3562 1373
Fragments arranged by size:
3562 2696 1503 1373
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1719 3407 3924 3969 4809 4946 5760 6184 6398
Size: 1688 517 45 840 137 814 424 214
Cuts at: 6398 6700 7081 1719
Size: 302 381 3772
Fragments arranged by size:
3772 1688 840 814 517 424 381 302
214 137 45
BsrBI CCG'CTC
Cuts at: 50 1428 2838 4611 5574 7426 7480 8964 9129
Size: 1378 1410 1773 963 1852 54 1484 165
Cuts at: 9129 50
Size: 55
Fragments arranged by size:
1852 1773 1484 1410 1378 963 165 55
54
BsrDI GCAATG_nn'
Cuts at: 320 1139 3221 7046 320
Size: 819 2082 3825 2408
Fragments arranged by size:
3825 2408 2082 819
BsrFI r'CCGG_y
Cuts at: 5978 6026 7132 7313 7522 5978
Size: 48 1106 181 209 7590
Fragments arranged by size:
7590 1106 209 181 48
BsrGI T'GTAC_A
Cuts at: 530 2480 530
Size: 1950 7184
BssSI C'ACGA_G
Cuts at: 944 2248 5192 7405 7763 944
Size: 1304 2944 2213 358 2315
Fragments arranged by size:
2944 2315 2213 1304 358
BstAPI GCAn_nnn'nTGC
Cuts at: 792 1393 2938 3105 6511 792
Size: 601 1545 167 3406 3415
Fragments arranged by size:
3415 3406 1545 601 167
BstBI TT'CG_AA
Cuts at: 7495 7495
Size: 9134
BstEII G'GTnAC_C
Cuts at: 2596 2596
Size: 9134
BstXI CCAn_nnnn'nTGG
Cuts at: 1291 2193 4086 1291
Size: 902 1893 6339
Fragments arranged by size:
6339 1893 902
BstYI r'GATC_y
Cuts at: 1 132 1677 2271 2321 2387 4211 6159 6626
Size: 131 1545 594 50 66 1824 1948 467
Cuts at: 6626 6649 6984 7230 8242 8328 8340 8686 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1948 1824 1545 1012 594 467 449 346
335 246 131 86 66 50 23 12
Bsu36I CC'TnA_GG
Cuts at: 740 740
Size: 9134
BtgI C'CryG_G
Cuts at: 107 234 1124 3683 4871 107
Size: 127 890 2559 1188 4370
Fragments arranged by size:
4370 2559 1188 890 127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1435 7084 7265 7428 1435
Size: 5649 181 163 3141
Fragments arranged by size:
5649 3141 181 163
BtsI GCAGTG_nn'
Cuts at: 2704 6377 2704
Size: 3673 5461
ClaI AT'CG_AT
Cuts at: 1664 1664
Size: 9134
DraI TTT'AAA
Cuts at: 2552 6133 2552
Size: 3581 5553
DrdI GACnn_nn'nnGTC
Cuts at: 163 3979 6840 7698 163
Size: 3816 2861 858 1599
Fragments arranged by size:
3816 2861 1599 858
EaeI y'GGCC_r
Cuts at: 1347 1946 2603 3275 3455 3488 6024 6719 6893
Size: 599 657 672 180 33 2536 695 174
Cuts at: 6893 7284 7311 8656 1347
Size: 391 27 1345 1825
Fragments arranged by size:
2536 1825 1345 695 672 657 599 391
180 174 33 27
EagI C'GGCC_G
Cuts at: 1347 6719 1347
Size: 5372 3762
EarI CTCTTCn'nnn_
Cuts at: 472 1154 2248 2771 2855 2915 2957 3146 3245
Size: 682 1094 523 84 60 42 189 99
Cuts at: 3245 4220 4571 4576 4601 4958 5242 5432 5539
Size: 975 351 5 25 357 284 190 107
Cuts at: 5539 6015 7157 7367 472
Size: 476 1142 210 2239
Fragments arranged by size:
2239 1142 1094 975 682 523 476 357
351 284 210 190 189 107 99 84
60 42 25 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4051 5023 5356 7342 7652 7798 8944 4051
Size: 972 333 1986 310 146 1146 4241
Fragments arranged by size:
4241 1986 1146 972 333 310 146
EcoICRI GAG'CTC
Cuts at: 2904 3441 3822 3990 4754 5001 6063 6146 2904
Size: 537 381 168 764 247 1062 83 5892
Fragments arranged by size:
5892 1062 764 537 381 247 168 83
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 1025 1175 2537 2746 2845 2933 3269 3553
Size: 583 150 1362 209 99 88 336 284
Cuts at: 3553 3685 3757 3835 3907 4028 4156 4228 4306
Size: 132 72 78 72 121 128 72 78
Cuts at: 4306 4414 4444 4474 4708 4849 4864 4915 5035
Size: 108 30 30 234 141 15 51 120
Cuts at: 5035 5098 5134 5443 5590 5611 5677 5698 5878
Size: 63 36 309 147 21 66 21 180
Cuts at: 5878 5893 5992 6079 6959 7391 8138 8406 442
Size: 15 99 87 880 432 747 268 1170
Fragments arranged by size:
1362 1170 880 747 583 432 336 309
284 268 234 209 180 150 147 141
132 128 121 120 108 99 99 88
87 78 78 72 72 72 66 63
51 36 30 30 21 21 15 15
EcoNI CCTnn'n_nnAGG
Cuts at: 3931 4477 3931
Size: 546 8588
EcoO109I rG'GnC_Cy
Cuts at: 3060 3882 3924 4407 4671 4806 4835 5082 5946
Size: 822 42 483 264 135 29 247 864
Cuts at: 5946 6273 3060
Size: 327 5921
Fragments arranged by size:
5921 864 822 483 327 264 247 135
42 29
EcoRI G'AATT_C
Cuts at: 2368 6138 2368
Size: 3770 5364
EcoRV GAT'ATC
Cuts at: 2565 2565
Size: 9134
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 7153 7153
Size: 9134
FspI TGC'GCA
Cuts at: 1271 6915 1271
Size: 5644 3490
HaeII r_GCGC'y
Cuts at: 4422 4521 5041 5363 5537 6033 6568 6816 7838
Size: 99 520 322 174 496 535 248 1022
Cuts at: 7838 8817 4422
Size: 979 4739
Fragments arranged by size:
4739 1022 979 535 520 496 322 248
174 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 607 639 838 870 1153 1185 1467 1499 2184
Size: 32 199 32 283 32 282 32 685
Cuts at: 2184 2216 2372 2379 2404 2411 2968 3000 3568
Size: 32 156 7 25 7 557 32 568
Cuts at: 3568 3600 4060 4092 5202 5234 6976 7006 7008
Size: 32 460 32 1110 32 1742 30 2
Cuts at: 7008 7038 607
Size: 30 2703
Fragments arranged by size:
2703 1742 1110 685 568 557 460 283
282 199 156 32 32 32 32 32
32 32 32 32 30 30 25 7
7 2
HincII GTy'rAC
Cuts at: 321 4902 5788 6102 6173 321
Size: 4581 886 314 71 3282
Fragments arranged by size:
4581 3282 886 314 71
HindIII A'AGCT_T
Cuts at: 401 1244 4387 4885 401
Size: 843 3143 498 4650
Fragments arranged by size:
4650 3143 843 498
KasI G'GCGC_C
Cuts at: 6029 6564 6812 6029
Size: 535 248 8351
Fragments arranged by size:
8351 535 248
KpnI G_GTAC'C
Cuts at: 6154 6154
Size: 9134
MfeI C'AATT_G
Cuts at: 4358 5272 4358
Size: 914 8220
MluI A'CGCG_T
Cuts at: 5881 8567 5881
Size: 2686 6448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4189 4556 6092 7805 7989 4189
Size: 367 1536 1713 184 5334
Fragments arranged by size:
5334 1713 1536 367 184
MscI TGG'CCA
Cuts at: 1948 2605 3277 3457 3490 6895 1948
Size: 657 672 180 33 3405 4187
Fragments arranged by size:
4187 3405 672 657 180 33
MslI CAynn'nnrTG
Cuts at: 569 2098 2719 7123 7250 569
Size: 1529 621 4404 127 2453
Fragments arranged by size:
4404 2453 1529 621 127
MspA1I CmG'CkG
Cuts at: 1593 2661 3111 3243 3453 3834 4221 4566 4842
Size: 1068 450 132 210 381 387 345 276
Cuts at: 4842 5034 5421 5676 5856 5913 6036 6245 6559
Size: 192 387 255 180 57 123 209 314
Cuts at: 6559 6919 7932 8177 1593
Size: 360 1013 245 2550
Fragments arranged by size:
2550 1068 1013 450 387 387 381 360
345 314 276 255 245 210 209 192
180 132 123 57
NaeI GCC'GGC
Cuts at: 6028 7315 6028
Size: 1287 7847
NarI GG'CG_CC
Cuts at: 6030 6565 6813 6030
Size: 535 248 8351
Fragments arranged by size:
8351 535 248
NcoI C'CATG_G
Cuts at: 107 234 1124 107
Size: 127 890 8117
Fragments arranged by size:
8117 890 127
NgoMIV G'CCGG_C
Cuts at: 6026 7313 6026
Size: 1287 7847
NheI G'CTAG_C
Cuts at: 5517 5517
Size: 9134
NsiI A_TGCA'T
Cuts at: 601 1693 601
Size: 1092 8042
NspI r_CATG'y
Cuts at: 392 603 2148 4908 6187 6687 7594 392
Size: 211 1545 2760 1279 500 907 1932
Fragments arranged by size:
2760 1932 1545 1279 907 500 211
PciI A'CATG_T
Cuts at: 7590 7590
Size: 9134
PflMI CCAn_nnn'nTGG
Cuts at: 4422 5568 4422
Size: 1146 7988
PfoI T'CCnGG_A
Cuts at: 1089 1115 1508 2312 2363 3782 3983 1089
Size: 26 393 804 51 1419 201 6240
Fragments arranged by size:
6240 1419 804 393 201 51 26
PmeI GTTT'AAAC
Cuts at: 6133 6133
Size: 9134
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2504 2536 8273 8305 2504
Size: 32 5737 32 3333
Fragments arranged by size:
5737 3333 32 32
PpuMI rG'GwC_Cy
Cuts at: 3060 3924 4835 5082 3060
Size: 864 911 247 7112
Fragments arranged by size:
7112 911 864 247
PsiI TTA'TAA
Cuts at: 481 481
Size: 9134
PspOMI G'GGCC_C
Cuts at: 4670 4805 4670
Size: 135 8999
PspXI vC'TCGA_Gb
Cuts at: 127 4675 5002 5611 127
Size: 4548 327 609 3650
Fragments arranged by size:
4548 3650 609 327
PstI C_TGCA'G
Cuts at: 1589 1685 3110 3404 3425 3452 3479 3932 4058
Size: 96 1425 294 21 27 27 453 126
Cuts at: 4058 4310 4478 5606 6181 6866 1589
Size: 252 168 1128 575 685 3857
Fragments arranged by size:
3857 1425 1128 685 575 453 294 252
168 126 96 27 27 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9134
PvuII CAG'CTG
Cuts at: 1593 3111 3243 3453 3834 4221 4566 4842 5421
Size: 1518 132 210 381 387 345 276 579
Cuts at: 5421 5676 5856 6036 6559 6919 1593
Size: 255 180 180 523 360 3808
Fragments arranged by size:
3808 1518 579 523 387 381 360 345
276 255 210 180 180 132
RsrII CG'GwC_CG
Cuts at: 7329 7329
Size: 9134
SacI G_AGCT'C
Cuts at: 2906 3443 3824 3992 4756 5003 6065 6148 2906
Size: 537 381 168 764 247 1062 83 5892
Fragments arranged by size:
5892 1062 764 537 381 247 168 83
SalI G'TCGA_C
Cuts at: 5786 6171 5786
Size: 385 8749
SapI GCTCTTCn'nnn_
Cuts at: 2771 4576 4601 7157 7367 2771
Size: 1805 25 2556 210 4538
Fragments arranged by size:
4538 2556 1805 210 25
SbfI CC_TGCA'GG
Cuts at: 3404 6181 3404
Size: 2777 6357
ScaI AGT'ACT
Cuts at: 8651 8651
Size: 9134
SfcI C'TryA_G
Cuts at: 33 1056 1198 1585 1681 3106 3400 3421 3448
Size: 1023 142 387 96 1425 294 21 27
Cuts at: 3448 3475 3928 4054 4150 4306 4474 5045 5491
Size: 27 453 126 96 156 168 571 446
Cuts at: 5491 5602 6177 6862 7855 8046 33
Size: 111 575 685 993 191 1121
Fragments arranged by size:
1425 1121 1023 993 685 575 571 453
446 387 294 191 168 156 142 126
111 96 96 27 27 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 2611 2611
Size: 9134
SfoI GGC'GCC
Cuts at: 6031 6566 6814 6031
Size: 535 248 8351
Fragments arranged by size:
8351 535 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9134
SmaI CCC'GGG
Cuts at: 4669 6156 4669
Size: 1487 7647
SmlI C'TyrA_G
Cuts at: 127 185 3169 3442 3630 3748 4138 4675 5002
Size: 58 2984 273 188 118 390 537 327
Cuts at: 5002 5611 5689 6289 7204 7696 7958 127
Size: 609 78 600 915 492 262 1303
Fragments arranged by size:
2984 1303 915 609 600 537 492 390
327 273 262 188 118 78 58
SnaBI TAC'GTA
Cuts at: 3706 3706
Size: 9134
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9134
SphI G_CATG'C
Cuts at: 603 6187 6687 603
Size: 5584 500 3050
Fragments arranged by size:
5584 3050 500
SrfI GCCC'GGGC
Cuts at: 4669 4669
Size: 9134
SspI AAT'ATT
Cuts at: 519 735 1212 2013 519
Size: 216 477 801 7640
Fragments arranged by size:
7640 801 477 216
StuI AGG'CCT
Cuts at: 2437 2614 2437
Size: 177 8957
StyI C'CwwG_G
Cuts at: 107 234 362 1124 3998 4694 5702 6268 6622
Size: 127 128 762 2874 696 1008 566 354
Cuts at: 6622 107
Size: 2619
Fragments arranged by size:
2874 2619 1008 762 696 566 354 128
127
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3409 6717 6850 7309 7518 3409
Size: 3308 133 459 209 5025
Fragments arranged by size:
5025 3308 459 209 133
TatI w'GTAC_w
Cuts at: 530 1728 2480 8649 530
Size: 1198 752 6169 1015
Fragments arranged by size:
6169 1198 1015 752
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2267 2299 3895 3927 8651 8683 2267
Size: 32 1596 32 4724 32 2718
Fragments arranged by size:
4724 2718 1596 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6931 6931
Size: 9134
XbaI T'CTAG_A
Cuts at: 3864 6165 3864
Size: 2301 6833
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1000 4281 4764 4890 5493 5652 6629 1000
Size: 3281 483 126 603 159 977 3505
Fragments arranged by size:
3505 3281 977 603 483 159 126
XhoI C'TCGA_G
Cuts at: 127 3169 4675 5002 5611 127
Size: 3042 1506 327 609 3650
Fragments arranged by size:
3650 3042 1506 609 327
XmaI C'CCGG_G
Cuts at: 4667 6154 4667
Size: 1487 7647
XmnI GAAnn'nnTTC
Cuts at: 1163 1212 2759 2966 5267 1163
Size: 49 1547 207 2301 5030
Fragments arranged by size:
5030 2301 1547 207 49
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NsiI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PsiI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AscI AvrII BaeI BmgBI BsiWI
BssHII BstZ17I CspCI DraIII FseI FspAI HpaI NdeI
NotI NruI PacI PasI PmlI PshAI PsrI SacII
SanDI SexAI SgrAI SwaI ZraI