Restriction map for
pF1KB0048
(Circular) (Six-base) MAPSORT of: pF1KB0048.seq Check: 385 from: 1 to: 8381
pF1KB0048 8381 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 1834 2583 3579 5419 1834
Size: 749 996 1840 4796
Fragments arranged by size:
4796 1840 996 749
Acc65I G'GTAC_C
Cuts at: 5397 5397
Size: 8381
AclI AA'CG_TT
Cuts at: 8221 8221
Size: 8381
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 287 3593 3956 4093 4103 4136 4307 4910 5215
Size: 3306 363 137 10 33 171 603 305
Cuts at: 5215 6206 6638 7385 7653 287
Size: 991 432 747 268 1015
Fragments arranged by size:
3306 1015 991 747 603 432 363 305
268 171 137 33 10
AflIII A'CryG_T
Cuts at: 5733 6837 7814 5733
Size: 1104 977 6300
Fragments arranged by size:
6300 1104 977
AgeI A'CCGG_T
Cuts at: 6769 6769
Size: 8381
AhdI GACnn_n'nnGTC
Cuts at: 591 1026 5247 5277 591
Size: 435 4221 30 3695
Fragments arranged by size:
4221 3695 435 30
AleI CACnn'nnGTG
Cuts at: 637 637
Size: 8381
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3182 3214 3182
Size: 32 8349
AlwNI CAG_nnn'CTG
Cuts at: 445 954 3559 5686 7253 445
Size: 509 2605 2127 1567 1573
Fragments arranged by size:
2605 2127 1573 1567 509
ApaI G_GGCC'C
Cuts at: 3620 3620
Size: 8381
ApaLI G'TGCA_C
Cuts at: 7151 7151
Size: 8381
ApoI r'AATT_y
Cuts at: 701 944 1106 1348 1469 2397 2919 4251 4470
Size: 243 162 242 121 928 522 1332 219
Cuts at: 4470 4711 5385 7734 7990 701
Size: 241 674 2349 256 1092
Fragments arranged by size:
2349 1332 1092 928 674 522 256 243
242 241 219 162 121
AseI AT'TA_AT
Cuts at: 20 20
Size: 8381
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8381
AvaI C'yCGr_G
Cuts at: 3097 3232 4979 5401 3097
Size: 135 1747 422 6077
Fragments arranged by size:
6077 1747 422 135
BamHI G'GATC_C
Cuts at: 3087 4046 5406 3087
Size: 959 1360 6062
Fragments arranged by size:
6062 1360 959
BanI G'GyrC_C
Cuts at: 3516 5397 5811 6059 6094 3516
Size: 1881 414 248 35 5803
Fragments arranged by size:
5803 1881 414 248 35
BanII G_rGCy'C
Cuts at: 159 1738 2948 3620 5395 6425 159
Size: 1579 1210 672 1775 1030 2115
Fragments arranged by size:
2115 1775 1579 1210 1030 672
BbeI G_GCGC'C
Cuts at: 5815 6063 5815
Size: 248 8133
BbsI GAAGACnn'nnnn_
Cuts at: 661 1882 2609 4754 5305 661
Size: 1221 727 2145 551 3737
Fragments arranged by size:
3737 2145 1221 727 551
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2506 2540 3213 3247 2506
Size: 34 673 34 7640
Fragments arranged by size:
7640 673 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1704 2246 6279 7040 8381 1704
Size: 542 4033 761 1341 1704
Fragments arranged by size:
4033 1704 1341 761 542
BclI T'GATC_A
Cuts at: 1041 1152 1464 2634 5901 1041
Size: 111 312 1170 3267 3521
Fragments arranged by size:
3521 3267 1170 312 111
BfrBI ATG'CAT
Cuts at: 2736 2736
Size: 8381
BglII A'GATC_T
Cuts at: 3895 5896 7933 3895
Size: 2001 2037 4343
Fragments arranged by size:
4343 2037 2001
BlpI GC'TnA_GC
Cuts at: 1811 1940 5493 1811
Size: 129 3553 4699
Fragments arranged by size:
4699 3553 129
Bme1580I G_kGCm'C
Cuts at: 3158 3620 6006 6099 7155 3158
Size: 462 2386 93 1056 4384
Fragments arranged by size:
4384 2386 1056 462 93
BmrI ACTGGGnnnn_n'
Cuts at: 1741 2223 4591 5593 5674 6009 8153 1741
Size: 482 2368 1002 81 335 2144 1969
Fragments arranged by size:
2368 2144 1969 1002 482 335 81
BmtI G_CTAG'C
Cuts at: 1822 1822
Size: 8381
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2742 6748 2742
Size: 4006 4375
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1896 2531 2837 2959 3402 3935 4619 1896
Size: 635 306 122 443 533 684 5658
Fragments arranged by size:
5658 684 635 533 443 306 122
Bpu10I CC'TnA_GC
Cuts at: 4553 4553
Size: 8381
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3246 3389 5033 5170 5557 6436 6928 7226 3246
Size: 143 1644 137 387 879 492 298 4401
Fragments arranged by size:
4401 1644 879 492 387 298 143 137
BsaI GGTCTCn'nnnn_
Cuts at: 1853 3221 3904 8085 1853
Size: 1368 683 4181 2149
Fragments arranged by size:
4181 2149 1368 683
BsaAI yAC'GTr
Cuts at: 3207 5734 3207
Size: 2527 5854
BsaBI GATnn'nnATC
Cuts at: 6 1589 2075 3092 3900 5895 7932 6
Size: 1583 486 1017 808 1995 2037 455
Fragments arranged by size:
2037 1995 1583 1017 808 486 455
BsaHI Gr'CG_yC
Cuts at: 5812 6060 6762 8258 5812
Size: 248 702 1496 5935
Fragments arranged by size:
5935 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1085 2679 3588 5570 6091 6769 7043 7190 7601
Size: 1594 909 1982 521 678 274 147 411
Cuts at: 7601 1085
Size: 1865
Fragments arranged by size:
1982 1865 1594 909 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1352 1382 1541 1571 2742 2772 2940 2970 3182
Size: 30 159 30 1171 30 168 30 212
Cuts at: 3182 3212 3400 3430 4335 4365 4617 4647 1352
Size: 30 188 30 905 30 252 30 5086
Fragments arranged by size:
5086 1171 905 252 212 188 168 159
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 579 2715 2956 4343 4346 5265 579
Size: 2136 241 1387 3 919 3695
Fragments arranged by size:
3695 2136 1387 919 241 3
BseYI C'CCAG_C
Cuts at: 1330 2883 2952 3596 4379 5806 6564 7141 7637
Size: 1553 69 644 783 1427 758 577 496
Cuts at: 7637 1330
Size: 2074
Fragments arranged by size:
2074 1553 1427 783 758 644 577 496
69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 4172 4727 4172
Size: 555 7826
BsiEI CG_ry'CG
Cuts at: 104 5923 5969 7177 104
Size: 5819 46 1208 1308
Fragments arranged by size:
5819 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3981 4434 5395 6173 6363 7155 3981
Size: 453 961 778 190 792 5207
Fragments arranged by size:
5207 961 792 778 453 190
BsmI GAATG_Cn'
Cuts at: 2738 2738
Size: 8381
BsmBI CGTCTCn'nnnn_
Cuts at: 923 2096 4412 923
Size: 1173 2316 4892
Fragments arranged by size:
4892 2316 1173
Bsp1286I G_dGCh'C
Cuts at: 159 1738 2948 3158 3620 3981 4434 5395 6006
Size: 1579 1210 210 462 361 453 961 611
Cuts at: 6006 6099 6173 6363 6425 7155 159
Size: 93 74 190 62 730 1385
Fragments arranged by size:
1579 1385 1210 961 730 611 462 453
361 210 190 93 74 62
BspEI T'CCGG_A
Cuts at: 1085 7601 1085
Size: 6516 1865
BspHI T'CATG_A
Cuts at: 1308 2739 7557 1308
Size: 1431 4818 2132
Fragments arranged by size:
4818 2132 1431
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3694 5431 5645 5947 6328 3694
Size: 1737 214 302 381 5747
Fragments arranged by size:
5747 1737 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1939 6673 6727 8211 8376 50
Size: 1889 4734 54 1484 165 55
Fragments arranged by size:
4734 1889 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 305 1080 3851 4193 4360 5004 6293 305
Size: 775 2771 342 167 644 1289 2393
Fragments arranged by size:
2771 2393 1289 775 644 342 167
BsrFI r'CCGG_y
Cuts at: 1004 6379 6560 6769 1004
Size: 5375 181 209 2616
Fragments arranged by size:
5375 2616 209 181
BsrGI T'GTAC_A
Cuts at: 1718 1827 4040 1718
Size: 109 2213 6059
Fragments arranged by size:
6059 2213 109
BssSI C'ACGA_G
Cuts at: 2099 6652 7010 2099
Size: 4553 358 3470
Fragments arranged by size:
4553 3470 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4195 5758 4195
Size: 1563 6818
BstBI TT'CG_AA
Cuts at: 1752 1914 6742 1752
Size: 162 4828 3391
Fragments arranged by size:
4828 3391 162
BstYI r'GATC_y
Cuts at: 1 3063 3087 3837 3895 4046 5322 5406 5873
Size: 3062 24 750 58 151 1276 84 467
Cuts at: 5873 5896 6231 6477 7489 7575 7587 7933 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
3062 1276 1012 750 467 449 346 335
246 151 86 84 58 24 23 12
BstZ17I GTA'TAC
Cuts at: 1835 1835
Size: 8381
BtgI C'CryG_G
Cuts at: 107 577 3723 5178 107
Size: 470 3146 1455 3310
Fragments arranged by size:
3310 3146 1455 470
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6331 6512 6675 6331
Size: 181 163 8037
Fragments arranged by size:
8037 181 163
BtsI GCAGTG_nn'
Cuts at: 3799 5146 5624 3799
Size: 1347 478 6556
Fragments arranged by size:
6556 1347 478
ClaI AT'CG_AT
Cuts at: 4647 4647
Size: 8381
DraI TTT'AAA
Cuts at: 5380 5380
Size: 8381
DraIII CAC_nnn'GTG
Cuts at: 4438 5224 4438
Size: 786 7595
DrdI GACnn_nn'nnGTC
Cuts at: 6087 6945 6087
Size: 858 7523
EaeI y'GGCC_r
Cuts at: 495 535 3379 4970 5966 6140 6531 6558 7903
Size: 40 2844 1591 996 174 391 27 1345
Cuts at: 7903 495
Size: 973
Fragments arranged by size:
2844 1591 1345 996 973 391 174 40
27
EagI C'GGCC_G
Cuts at: 5966 5966
Size: 8381
EarI CTCTTCn'nnn_
Cuts at: 1094 1220 3428 3464 3539 3824 4931 6404 6614
Size: 126 2208 36 75 285 1107 1473 210
Cuts at: 6614 1094
Size: 2861
Fragments arranged by size:
2861 2208 1473 1107 285 210 126 75
36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1871 2500 3519 6589 6899 7045 8191 1871
Size: 629 1019 3070 310 146 1146 2061
Fragments arranged by size:
3070 2061 1146 1019 629 310 146
EcoICRI GAG'CTC
Cuts at: 5393 5393
Size: 8381
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 287 1896 2531 2837 2959 3402 3593 3935 3956
Size: 1609 635 306 122 443 191 342 21
Cuts at: 3956 4093 4103 4136 4307 4619 4910 5215 6206
Size: 137 10 33 171 312 291 305 991
Cuts at: 6206 6638 7385 7653 287
Size: 432 747 268 1015
Fragments arranged by size:
1609 1015 991 747 635 443 432 342
312 306 305 291 268 191 171 137
122 33 21 10
EcoNI CCTnn'n_nnAGG
Cuts at: 3611 3611
Size: 8381
EcoO109I rG'GnC_Cy
Cuts at: 2624 3616 5520 2624
Size: 992 1904 5485
Fragments arranged by size:
5485 1904 992
EcoRI G'AATT_C
Cuts at: 4470 5385 4470
Size: 915 7466
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1467 1540 6400 1467
Size: 73 4860 3448
Fragments arranged by size:
4860 3448 73
FspI TGC'GCA
Cuts at: 6162 6162
Size: 8381
HaeII r_GCGC'y
Cuts at: 5815 6063 7085 8064 5815
Size: 248 1022 979 6132
Fragments arranged by size:
6132 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 577 609 1012 1044 1775 1807 1918 1950 2286
Size: 32 403 32 731 32 111 32 336
Cuts at: 2286 2318 2515 2547 3400 3432 5157 5189 5233
Size: 32 197 32 853 32 1725 32 44
Cuts at: 5233 5263 5265 5295 6223 6253 6255 6285 577
Size: 30 2 30 928 30 2 30 2673
Fragments arranged by size:
2673 1725 928 853 731 403 336 197
111 44 32 32 32 32 32 32
32 32 30 30 30 30 2 2
HincII GTy'rAC
Cuts at: 306 513 5420 306
Size: 207 4907 3267
Fragments arranged by size:
4907 3267 207
HindIII A'AGCT_T
Cuts at: 2843 2843
Size: 8381
HpaI GTT'AAC
Cuts at: 306 513 306
Size: 207 8174
KasI G'GCGC_C
Cuts at: 5811 6059 5811
Size: 248 8133
KpnI G_GTAC'C
Cuts at: 5401 5401
Size: 8381
MfeI C'AATT_G
Cuts at: 2240 2240
Size: 8381
MluI A'CGCG_T
Cuts at: 7814 7814
Size: 8381
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1380 3091 5294 5319 7052 7236 1380
Size: 1711 2203 25 1733 184 2525
Fragments arranged by size:
2525 2203 1733 1711 184 25
MscI TGG'CCA
Cuts at: 4972 6142 4972
Size: 1170 7211
MslI CAynn'nnrTG
Cuts at: 637 1148 1313 6370 6497 637
Size: 511 165 5057 127 2521
Fragments arranged by size:
5057 2521 511 165 127
MspA1I CmG'CkG
Cuts at: 265 579 1026 2460 3736 3747 4146 4930 5492
Size: 314 447 1434 1276 11 399 784 562
Cuts at: 5492 5806 6166 7179 7424 265
Size: 314 360 1013 245 1222
Fragments arranged by size:
1434 1276 1222 1013 784 562 447 399
360 314 314 245 11
NaeI GCC'GGC
Cuts at: 6562 6562
Size: 8381
NarI GG'CG_CC
Cuts at: 5812 6060 5812
Size: 248 8133
NcoI C'CATG_G
Cuts at: 107 3723 5178 107
Size: 3616 1455 3310
Fragments arranged by size:
3616 3310 1455
NgoMIV G'CCGG_C
Cuts at: 6560 6560
Size: 8381
NheI G'CTAG_C
Cuts at: 1818 1818
Size: 8381
NsiI A_TGCA'T
Cuts at: 2738 2738
Size: 8381
NspI r_CATG'y
Cuts at: 337 1147 2182 3061 5434 5934 6841 337
Size: 810 1035 879 2373 500 907 1877
Fragments arranged by size:
2373 1877 1035 907 879 810 500
PasI CC'CwG_GG
Cuts at: 3151 3151
Size: 8381
PciI A'CATG_T
Cuts at: 6837 6837
Size: 8381
PflMI CCAn_nnn'nTGG
Cuts at: 493 1075 1285 3559 4603 493
Size: 582 210 2274 1044 4271
Fragments arranged by size:
4271 2274 1044 582 210
PfoI T'CCnGG_A
Cuts at: 159 646 5316 159
Size: 487 4670 3224
Fragments arranged by size:
4670 3224 487
PmeI GTTT'AAAC
Cuts at: 5380 5380
Size: 8381
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3182 3214 7520 7552 3182
Size: 32 4306 32 4011
Fragments arranged by size:
4306 4011 32 32
PspOMI G'GGCC_C
Cuts at: 3616 3616
Size: 8381
PspXI vC'TCGA_Gb
Cuts at: 3232 3232
Size: 8381
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1403 1435 2988 3020 1403
Size: 32 1553 32 6764
Fragments arranged by size:
6764 1553 32 32
PstI C_TGCA'G
Cuts at: 1440 3630 3735 5428 6113 1440
Size: 2190 105 1693 685 3708
Fragments arranged by size:
3708 2190 1693 685 105
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8381
PvuII CAG'CTG
Cuts at: 265 2460 3736 4146 4930 5806 6166 265
Size: 2195 1276 410 784 876 360 2480
Fragments arranged by size:
2480 2195 1276 876 784 410 360
RsrII CG'GwC_CG
Cuts at: 6576 6576
Size: 8381
SacI G_AGCT'C
Cuts at: 5395 5395
Size: 8381
SacII CC_GC'GG
Cuts at: 580 580
Size: 8381
SalI G'TCGA_C
Cuts at: 5418 5418
Size: 8381
SapI GCTCTTCn'nnn_
Cuts at: 3464 3539 6404 6614 3464
Size: 75 2865 210 5231
Fragments arranged by size:
5231 2865 210 75
SbfI CC_TGCA'GG
Cuts at: 5428 5428
Size: 8381
ScaI AGT'ACT
Cuts at: 3006 7898 3006
Size: 4892 3489
SfcI C'TryA_G
Cuts at: 33 277 1436 3626 3731 5424 6109 7102 7293
Size: 244 1159 2190 105 1693 685 993 191
Cuts at: 7293 33
Size: 1121
Fragments arranged by size:
2190 1693 1159 1121 993 685 244 191
105
SfoI GGC'GCC
Cuts at: 5813 6061 5813
Size: 248 8133
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8381
SmaI CCC'GGG
Cuts at: 5403 5403
Size: 8381
SmlI C'TyrA_G
Cuts at: 3232 3261 3404 5048 5185 5536 6451 6943 7205
Size: 29 143 1644 137 351 915 492 262
Cuts at: 7205 3232
Size: 4408
Fragments arranged by size:
4408 1644 915 492 351 262 143 137
29
SnaBI TAC'GTA
Cuts at: 3207 3207
Size: 8381
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8381
SphI G_CATG'C
Cuts at: 1147 5434 5934 1147
Size: 4287 500 3594
Fragments arranged by size:
4287 3594 500
SspI AAT'ATT
Cuts at: 738 1406 3655 4132 738
Size: 668 2249 477 4987
Fragments arranged by size:
4987 2249 668 477
StuI AGG'CCT
Cuts at: 990 2007 990
Size: 1017 7364
StyI C'CwwG_G
Cuts at: 107 1442 3684 3723 5178 5515 5869 107
Size: 1335 2242 39 1455 337 354 2619
Fragments arranged by size:
2619 2242 1455 1335 354 337 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 305 3164 4085 4175 5964 6097 6556 6765 305
Size: 2859 921 90 1789 133 459 209 1921
Fragments arranged by size:
2859 1921 1789 921 459 209 133 90
TatI w'GTAC_w
Cuts at: 1718 1827 3004 3861 4040 7896 1718
Size: 109 1177 857 179 3856 2203
Fragments arranged by size:
3856 2203 1177 857 179 109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1352 1384 1541 1573 2940 2972 4335 4367 5212
Size: 32 157 32 1367 32 1363 32 845
Cuts at: 5212 5244 7898 7930 1352
Size: 32 2654 32 1803
Fragments arranged by size:
2654 1803 1367 1363 845 157 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6178 6178
Size: 8381
XbaI T'CTAG_A
Cuts at: 1422 3697 5412 1422
Size: 2275 1715 4391
Fragments arranged by size:
4391 2275 1715
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1338 2049 2125 5876 1338
Size: 711 76 3751 3843
Fragments arranged by size:
3843 3751 711 76
XhoI C'TCGA_G
Cuts at: 3232 3232
Size: 8381
XmaI C'CCGG_G
Cuts at: 5401 5401
Size: 8381
XmnI GAAnn'nnTTC
Cuts at: 2438 4015 2438
Size: 1577 6804
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BstZ17I BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AscI AvrII BaeI
BbvCI BglI BmgBI BsiWI BssHII BstEII BstXI Bsu36I
CspCI EcoRV FseI FspAI NdeI NotI NruI PacI
PmlI PpuMI PshAI PsiI SanDI SexAI SfiI SgrAI
SrfI SwaI ZraI