Restriction map for
pF1KB0051
(Circular) (Six-base) MAPSORT of: pF1KB0051.seq Check: 1582 from: 1 to: 8486
pF1KB0051 8486 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 312 312
Size: 8486
AatII G_ACGT'C
Cuts at: 2891 2891
Size: 8486
AccI GT'mk_AC
Cuts at: 3821 5524 3821
Size: 1703 6783
Acc65I G'GTAC_C
Cuts at: 2282 5150 5502 2282
Size: 2868 352 5266
Fragments arranged by size:
5266 2868 352
AclI AA'CG_TT
Cuts at: 8326 8326
Size: 8486
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 240 1243 1465 3544 4641 6311 6743 7490 7758
Size: 1003 222 2079 1097 1670 432 747 268
Cuts at: 7758 240
Size: 968
Fragments arranged by size:
2079 1670 1097 1003 968 747 432 268
222
AflIII A'CryG_T
Cuts at: 308 377 518 5174 5225 5838 6942 7919 308
Size: 69 141 4656 51 613 1104 977 875
Fragments arranged by size:
4656 1104 977 875 613 141 69 51
AgeI A'CCGG_T
Cuts at: 6874 6874
Size: 8486
AhdI GACnn_n'nnGTC
Cuts at: 519 749 4671 519
Size: 230 3922 4334
Fragments arranged by size:
4334 3922 230
AleI CACnn'nnGTG
Cuts at: 1684 2485 4462 1684
Size: 801 1977 5708
Fragments arranged by size:
5708 1977 801
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 422 3700 5383 422
Size: 3278 1683 3525
Fragments arranged by size:
3525 3278 1683
AlwNI CAG_nnn'CTG
Cuts at: 2067 2127 2559 5094 5791 7358 2067
Size: 60 432 2535 697 1567 3195
Fragments arranged by size:
3195 2535 1567 697 432 60
ApaI G_GGCC'C
Cuts at: 635 3275 3368 635
Size: 2640 93 5753
Fragments arranged by size:
5753 2640 93
ApaLI G'TGCA_C
Cuts at: 5343 7256 5343
Size: 1913 6573
ApoI r'AATT_y
Cuts at: 5490 7839 8095 5490
Size: 2349 256 5881
Fragments arranged by size:
5881 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8486
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8486
AvaI C'yCGr_G
Cuts at: 158 1416 1924 2212 2778 3210 3722 5014 5060
Size: 1258 508 288 566 432 512 1292 46
Cuts at: 5060 5506 158
Size: 446 3138
Fragments arranged by size:
3138 1292 1258 566 512 508 446 432
288 46
BamHI G'GATC_C
Cuts at: 5511 5511
Size: 8486
BanI G'GyrC_C
Cuts at: 406 1576 2282 2598 4439 4960 5150 5210 5502
Size: 1170 706 316 1841 521 190 60 292
Cuts at: 5502 5916 6164 6199 406
Size: 414 248 35 2693
Fragments arranged by size:
2693 1841 1170 706 521 414 316 292
248 190 60 35
BanII G_rGCy'C
Cuts at: 635 1342 1475 1811 1859 2213 2785 3275 3368
Size: 707 133 336 48 354 572 490 93
Cuts at: 3368 3521 4391 5015 5087 5500 6530 635
Size: 153 870 624 72 413 1030 2591
Fragments arranged by size:
2591 1030 870 707 624 572 490 413
354 336 153 133 93 72 48
BbeI G_GCGC'C
Cuts at: 4443 5214 5920 6168 4443
Size: 771 706 248 6761
Fragments arranged by size:
6761 771 706 248
BbsI GAAGACnn'nnnn_
Cuts at: 759 1012 1752 4272 4585 759
Size: 253 740 2520 313 4660
Fragments arranged by size:
4660 2520 740 313 253
BbvCI CC'TCA_GC
Cuts at: 1141 3276 4712 1141
Size: 2135 1436 4915
Fragments arranged by size:
4915 2135 1436
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2572 2606 2572
Size: 34 8452
BciVI GTATCCnnnnn_n'
Cuts at: 364 4081 6384 7145 8486 364
Size: 3717 2303 761 1341 364
Fragments arranged by size:
3717 2303 1341 761 364
BclI T'GATC_A
Cuts at: 6006 6006
Size: 8486
BfrBI ATG'CAT
Cuts at: 2735 3899 5394 2735
Size: 1164 1495 5827
Fragments arranged by size:
5827 1495 1164
BglI GCCn_nnn'nGGC
Cuts at: 1540 3073 1540
Size: 1533 6953
BglII A'GATC_T
Cuts at: 3830 6001 8038 3830
Size: 2171 2037 4278
Fragments arranged by size:
4278 2171 2037
BlpI GC'TnA_GC
Cuts at: 1469 1976 3688 3763 4390 4555 5598 1469
Size: 507 1712 75 627 165 1043 4357
Fragments arranged by size:
4357 1712 1043 627 507 165 75
Bme1580I G_kGCm'C
Cuts at: 635 895 1736 2603 3275 3317 3368 3752 5347
Size: 260 841 867 672 42 51 384 1595
Cuts at: 5347 6111 6204 7260 635
Size: 764 93 1056 1861
Fragments arranged by size:
1861 1595 1056 867 841 764 672 384
260 93 51 42
BmgBI CAC'GTC
Cuts at: 2294 3267 3564 3684 4824 5177 2294
Size: 973 297 120 1140 353 5603
Fragments arranged by size:
5603 1140 973 353 297 120
BmrI ACTGGGnnnn_n'
Cuts at: 1998 5698 5779 6114 8258 1998
Size: 3700 81 335 2144 2226
Fragments arranged by size:
3700 2226 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 666 2619 3525 5052 6853 666
Size: 1953 906 1527 1801 2299
Fragments arranged by size:
2299 1953 1801 1527 906
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1322 1758 2035 2044 2089 2134 2157 2167 2560
Size: 436 277 9 45 45 23 10 393
Cuts at: 2560 2589 2899 3862 4010 4521 4567 4633 4671
Size: 29 310 963 148 511 46 66 38
Cuts at: 4671 4753 5185 5436 1322
Size: 82 432 251 4372
Fragments arranged by size:
4372 963 511 436 432 393 310 277
251 148 82 66 46 45 45 38
29 23 10 9
Bpu10I CC'TnA_GC
Cuts at: 1132 1141 2548 2980 3276 4712 1132
Size: 9 1407 432 296 1436 4906
Fragments arranged by size:
4906 1436 1407 432 296 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 886 1059 1119 2317 5302 5662 6541 7033 7331
Size: 173 60 1198 2985 360 879 492 298
Cuts at: 7331 886
Size: 2041
Fragments arranged by size:
2985 2041 1198 879 492 360 298 173
60
BsaI GGTCTCn'nnnn_
Cuts at: 736 799 1120 1474 2639 4024 4609 8190 736
Size: 63 321 354 1165 1385 585 3581 1032
Fragments arranged by size:
3581 1385 1165 1032 585 354 321 63
BsaAI yAC'GTr
Cuts at: 639 4404 5839 639
Size: 3765 1435 3286
Fragments arranged by size:
3765 3286 1435
BsaBI GATnn'nnATC
Cuts at: 6 6000 8037 6
Size: 5994 2037 455
Fragments arranged by size:
5994 2037 455
BsaHI Gr'CG_yC
Cuts at: 244 1969 2795 2888 3116 3436 4440 5211 5917
Size: 1725 826 93 228 320 1004 771 706
Cuts at: 5917 6165 6867 8363 244
Size: 248 702 1496 367
Fragments arranged by size:
1725 1496 1004 826 771 706 702 367
320 248 228 93
BsaWI w'CCGG_w
Cuts at: 976 1517 1763 1910 2267 2895 4084 4112 5675
Size: 541 246 147 357 628 1189 28 1563
Cuts at: 5675 6196 6874 7148 7295 7706 976
Size: 521 678 274 147 411 1756
Fragments arranged by size:
1756 1563 1189 678 628 541 521 411
357 274 246 147 147 28
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2062 2092 4503 4533 4630 4660 2062
Size: 30 2411 30 97 30 5888
Fragments arranged by size:
5888 2411 97 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 358 1039 1441 1504 1762 2110 2212 2215 2218
Size: 681 402 63 258 348 102 3 3
Cuts at: 2218 2221 2827 2845 2848 2851 2854 2857 2860
Size: 3 606 18 3 3 3 3 3
Cuts at: 2860 2863 2866 2869 2878 2881 2884 3319 3904
Size: 3 3 3 9 3 3 435 585
Cuts at: 3904 4498 4674 4686 4914 358
Size: 594 176 12 228 3930
Fragments arranged by size:
3930 681 606 594 585 435 402 348
258 228 176 102 63 18 12 9
3 3 3 3 3 3 3 3
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 627 856 1858 2000 4098 4244 4622 5087 5183
Size: 229 1002 142 2098 146 378 465 96
Cuts at: 5183 5911 6669 7246 7742 627
Size: 728 758 577 496 1371
Fragments arranged by size:
2098 1371 1002 758 728 577 496 465
378 229 146 142 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3281 4224 5140 3281
Size: 943 916 6627
Fragments arranged by size:
6627 943 916
BsiEI CG_ry'CG
Cuts at: 104 2586 2919 2937 3869 5135 6028 6074 7282
Size: 2482 333 18 932 1266 893 46 1208
Cuts at: 7282 104
Size: 1308
Fragments arranged by size:
2482 1308 1266 1208 932 893 333 46
18
BsiHKAI G_wGCw'C
Cuts at: 620 1475 1754 2903 3689 4391 5267 5347 5500
Size: 855 279 1149 786 702 876 80 153
Cuts at: 5500 6278 6468 7260 620
Size: 778 190 792 1846
Fragments arranged by size:
1846 1149 876 855 792 786 778 702
279 190 153 80
BsmBI CGTCTCn'nnnn_
Cuts at: 235 1960 2300 2455 235
Size: 1725 340 155 6266
Fragments arranged by size:
6266 1725 340 155
Bsp1286I G_dGCh'C
Cuts at: 620 635 895 1342 1475 1736 1754 1811 1859
Size: 15 260 447 133 261 18 57 48
Cuts at: 1859 2213 2603 2785 2903 3275 3317 3368 3521
Size: 354 390 182 118 372 42 51 153
Cuts at: 3521 3689 3752 4391 5015 5087 5267 5347 5500
Size: 168 63 639 624 72 180 80 153
Cuts at: 5500 6111 6204 6278 6468 6530 7260 620
Size: 611 93 74 190 62 730 1846
Fragments arranged by size:
1846 730 639 624 611 447 390 372
354 261 260 190 182 180 168 153
153 133 118 93 80 74 72 63
62 57 51 48 42 18 15
BspEI T'CCGG_A
Cuts at: 976 1517 1763 2267 2895 4084 7706 976
Size: 541 246 504 628 1189 3622 1756
Fragments arranged by size:
3622 1756 1189 628 541 504 246
BspHI T'CATG_A
Cuts at: 7662 7662
Size: 8486
BspMI ACCTGCnnnn'nnnn_
Cuts at: 312 2117 2315 2438 2996 3821 4601 5162 5536
Size: 1805 198 123 558 825 780 561 374
Cuts at: 5536 5750 6052 6433 312
Size: 214 302 381 2365
Fragments arranged by size:
2365 1805 825 780 561 558 381 374
302 214 198 123
BsrBI CCG'CTC
Cuts at: 50 867 2091 2238 3209 4704 5256 6778 6832
Size: 817 1224 147 971 1495 552 1522 54
Cuts at: 6832 8316 8481 50
Size: 1484 165 55
Fragments arranged by size:
1522 1495 1484 1224 971 817 552 165
147 55 54
BsrDI GCAATG_nn'
Cuts at: 1182 6398 1182
Size: 5216 3270
BsrFI r'CCGG_y
Cuts at: 409 2638 2936 4248 4436 6484 6665 6874 409
Size: 2229 298 1312 188 2048 181 209 2021
Fragments arranged by size:
2229 2048 2021 1312 298 209 188 181
BsrGI T'GTAC_A
Cuts at: 5228 5228
Size: 8486
BssSI C'ACGA_G
Cuts at: 1396 6757 7115 1396
Size: 5361 358 2767
Fragments arranged by size:
5361 2767 358
BstAPI GCAn_nnn'nTGC
Cuts at: 624 3896 4593 5313 5863 624
Size: 3272 697 720 550 3247
Fragments arranged by size:
3272 3247 720 697 550
BstBI TT'CG_AA
Cuts at: 6847 6847
Size: 8486
BstEII G'GTnAC_C
Cuts at: 1233 1797 2634 3585 4179 1233
Size: 564 837 951 594 5540
Fragments arranged by size:
5540 951 837 594 564
BstXI CCAn_nnnn'nTGG
Cuts at: 720 1270 1326 1521 2355 720
Size: 550 56 195 834 6851
Fragments arranged by size:
6851 834 550 195 56
BstYI r'GATC_y
Cuts at: 1 1166 3830 5117 5511 5978 6001 6336 6582
Size: 1165 2664 1287 394 467 23 335 246
Cuts at: 6582 7594 7680 7692 8038 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2664 1287 1165 1012 467 449 394 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 293 3697 4972 293
Size: 3404 1275 3807
Fragments arranged by size:
3807 3404 1275
BtgI C'CryG_G
Cuts at: 107 530 719 818 878 2492 3191 3869 3882
Size: 423 189 99 60 1614 699 678 13
Cuts at: 3882 4677 5249 5355 107
Size: 795 572 106 3238
Fragments arranged by size:
3238 1614 795 699 678 572 423 189
106 99 60 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3558 6436 6617 6780 3558
Size: 2878 181 163 5264
Fragments arranged by size:
5264 2878 181 163
BtsI GCAGTG_nn'
Cuts at: 622 1752 3317 3584 3798 4344 5276 5729 622
Size: 1130 1565 267 214 546 932 453 3379
Fragments arranged by size:
3379 1565 1130 932 546 453 267 214
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 198 233 198
Size: 35 8451
DraI TTT'AAA
Cuts at: 5485 5485
Size: 8486
DraIII CAC_nnn'GTG
Cuts at: 3018 3018
Size: 8486
DrdI GACnn_nn'nnGTC
Cuts at: 1831 3817 4038 5439 6192 7050 1831
Size: 1986 221 1401 753 858 3267
Fragments arranged by size:
3267 1986 1401 858 753 221
EaeI y'GGCC_r
Cuts at: 110 725 1541 1556 2489 2916 3065 3642 3866
Size: 615 816 15 933 427 149 577 224
Cuts at: 3866 4763 4907 4996 5273 5426 6071 6245 6636
Size: 897 144 89 277 153 645 174 391
Cuts at: 6636 6663 8008 110
Size: 27 1345 588
Fragments arranged by size:
1345 933 897 816 645 615 588 577
427 391 277 224 174 153 149 144
89 27 15
EagI C'GGCC_G
Cuts at: 2916 3866 6071 2916
Size: 950 2205 5331
Fragments arranged by size:
5331 2205 950
EarI CTCTTCn'nnn_
Cuts at: 490 2726 3086 3221 4086 6509 6719 490
Size: 2236 360 135 865 2423 210 2257
Fragments arranged by size:
2423 2257 2236 865 360 210 135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 133 945 1518 2657 3109 3382 6694 7004 7150
Size: 812 573 1139 452 273 3312 310 146
Cuts at: 7150 8296 133
Size: 1146 323
Fragments arranged by size:
3312 1146 1139 812 573 452 323 310
273 146
EcoICRI GAG'CTC
Cuts at: 1473 4389 5498 1473
Size: 2916 1109 4461
Fragments arranged by size:
4461 2916 1109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 240 1243 1322 1465 1758 2035 2044 2089 2134
Size: 1003 79 143 293 277 9 45 45
Cuts at: 2134 2157 2167 2560 2589 2899 3544 3862 4010
Size: 23 10 393 29 310 645 318 148
Cuts at: 4010 4521 4567 4633 4641 4671 4753 5185 5436
Size: 511 46 66 8 30 82 432 251
Cuts at: 5436 6311 6743 7490 7758 240
Size: 875 432 747 268 968
Fragments arranged by size:
1003 968 875 747 645 511 432 432
393 318 310 293 277 268 251 148
143 82 79 66 46 45 45 30
29 23 10 9 8
EcoNI CCTnn'n_nnAGG
Cuts at: 904 943 1627 2335 3676 3919 4564 4567 5368
Size: 39 684 708 1341 243 645 3 801
Cuts at: 5368 904
Size: 4022
Fragments arranged by size:
4022 1341 801 708 684 645 243 39
3
EcoO109I rG'GnC_Cy
Cuts at: 480 631 632 645 687 1194 1248 1306 1632
Size: 151 1 13 42 507 54 58 326
Cuts at: 1632 2304 2874 2922 2966 3096 3271 3272 3365
Size: 672 570 48 44 130 175 1 93
Cuts at: 3365 3541 3701 3790 3905 4505 4881 4976 5625
Size: 176 160 89 115 600 376 95 649
Cuts at: 5625 480
Size: 3341
Fragments arranged by size:
3341 672 649 600 570 507 376 326
176 175 160 151 130 115 95 93
89 58 54 48 44 42 13 1
1
EcoRI G'AATT_C
Cuts at: 5490 5490
Size: 8486
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6505 6505
Size: 8486
FspI TGC'GCA
Cuts at: 6267 6267
Size: 8486
HaeII r_GCGC'y
Cuts at: 795 2257 4437 4443 4694 5094 5196 5214 5920
Size: 1462 2180 6 251 400 102 18 706
Cuts at: 5920 6168 7190 8169 795
Size: 248 1022 979 1112
Fragments arranged by size:
2180 1462 1112 1022 979 706 400 251
248 102 18 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 472 504 505 537 735 767 3094 3126 4184
Size: 32 1 32 198 32 2327 32 1058
Cuts at: 4184 4216 4503 4535 4630 4657 4662 4689 4881
Size: 32 287 32 95 27 5 27 192
Cuts at: 4881 4913 6328 6358 6360 6390 472
Size: 32 1415 30 2 30 2568
Fragments arranged by size:
2568 2327 1415 1058 287 198 192 95
32 32 32 32 32 32 32 30
30 27 27 5 2 1
HincII GTy'rAC
Cuts at: 222 1040 1620 3291 5525 222
Size: 818 580 1671 2234 3183
Fragments arranged by size:
3183 2234 1671 818 580
HindIII A'AGCT_T
Cuts at: 252 252
Size: 8486
HpaI GTT'AAC
Cuts at: 222 222
Size: 8486
KasI G'GCGC_C
Cuts at: 4439 5210 5916 6164 4439
Size: 771 706 248 6761
Fragments arranged by size:
6761 771 706 248
KpnI G_GTAC'C
Cuts at: 2286 5154 5506 2286
Size: 2868 352 5266
Fragments arranged by size:
5266 2868 352
MluI A'CGCG_T
Cuts at: 308 7919 308
Size: 7611 875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2816 7157 7341 2816
Size: 4341 184 3961
Fragments arranged by size:
4341 3961 184
MscI TGG'CCA
Cuts at: 1558 3644 4909 5275 6247 1558
Size: 2086 1265 366 972 3797
Fragments arranged by size:
3797 2086 1265 972 366
MslI CAynn'nnrTG
Cuts at: 193 427 733 1519 1684 1888 2032 2353 2485
Size: 234 306 786 165 204 144 321 132
Cuts at: 2485 2734 3577 4462 6475 6602 193
Size: 249 843 885 2013 127 2077
Fragments arranged by size:
2077 2013 885 843 786 321 306 249
234 204 165 144 132 127
MspA1I CmG'CkG
Cuts at: 1172 1638 1975 2124 3980 4626 4755 5357 5597
Size: 466 337 149 1856 646 129 602 240
Cuts at: 5597 5911 6271 7284 7529 1172
Size: 314 360 1013 245 2129
Fragments arranged by size:
2129 1856 1013 646 602 466 360 337
314 245 240 149 129
NaeI GCC'GGC
Cuts at: 411 4250 4438 6667 411
Size: 3839 188 2229 2230
Fragments arranged by size:
3839 2230 2229 188
NarI GG'CG_CC
Cuts at: 4440 5211 5917 6165 4440
Size: 771 706 248 6761
Fragments arranged by size:
6761 771 706 248
NcoI C'CATG_G
Cuts at: 107 4677 107
Size: 4570 3916
NgoMIV G'CCGG_C
Cuts at: 409 4248 4436 6665 409
Size: 3839 188 2229 2230
Fragments arranged by size:
3839 2230 2229 188
NsiI A_TGCA'T
Cuts at: 2737 3901 5396 2737
Size: 1164 1495 5827
Fragments arranged by size:
5827 1495 1164
NspI r_CATG'y
Cuts at: 361 522 2735 3894 5229 5394 5539 6039 6946
Size: 161 2213 1159 1335 165 145 500 907
Cuts at: 6946 361
Size: 1901
Fragments arranged by size:
2213 1901 1335 1159 907 500 165 161
145
PasI CC'CwG_GG
Cuts at: 939 1263 4423 939
Size: 324 3160 5002
Fragments arranged by size:
5002 3160 324
PciI A'CATG_T
Cuts at: 518 5225 6942 518
Size: 4707 1717 2062
Fragments arranged by size:
4707 2062 1717
PflMI CCAn_nnn'nTGG
Cuts at: 2067 2067
Size: 8486
PfoI T'CCnGG_A
Cuts at: 683 1052 2060 2501 3786 4451 4634 4877 5111
Size: 369 1008 441 1285 665 183 243 234
Cuts at: 5111 5162 683
Size: 51 4007
Fragments arranged by size:
4007 1285 1008 665 441 369 243 234
183 51
PmeI GTTT'AAAC
Cuts at: 5485 5485
Size: 8486
PmlI CAC'GTG
Cuts at: 4404 4404
Size: 8486
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7625 7657 7625
Size: 32 8454
PpuMI rG'GwC_Cy
Cuts at: 480 645 687 1194 1248 1306 1632 2304 2874
Size: 165 42 507 54 58 326 672 570
Cuts at: 2874 2922 3790 3905 4881 4976 480
Size: 48 868 115 976 95 3990
Fragments arranged by size:
3990 976 868 672 570 507 326 165
115 95 58 54 48 42
PspOMI G'GGCC_C
Cuts at: 631 3271 3364 631
Size: 2640 93 5753
Fragments arranged by size:
5753 2640 93
PstI C_TGCA'G
Cuts at: 356 1110 2159 2595 3434 4568 5372 5533 6218
Size: 754 1049 436 839 1134 804 161 685
Cuts at: 6218 356
Size: 2624
Fragments arranged by size:
2624 1134 1049 839 804 754 685 436
161
PvuI CG_AT'CG
Cuts at: 104 5135 104
Size: 5031 3455
PvuII CAG'CTG
Cuts at: 1975 5911 6271 1975
Size: 3936 360 4190
Fragments arranged by size:
4190 3936 360
RsrII CG'GwC_CG
Cuts at: 6681 6681
Size: 8486
SacI G_AGCT'C
Cuts at: 1475 4391 5500 1475
Size: 2916 1109 4461
Fragments arranged by size:
4461 2916 1109
SacII CC_GC'GG
Cuts at: 5358 5358
Size: 8486
SalI G'TCGA_C
Cuts at: 5523 5523
Size: 8486
SanDI GG'GwC_CC
Cuts at: 2922 3790 4881 2922
Size: 868 1091 6527
Fragments arranged by size:
6527 1091 868
SapI GCTCTTCn'nnn_
Cuts at: 3221 6509 6719 3221
Size: 3288 210 4988
Fragments arranged by size:
4988 3288 210
SbfI CC_TGCA'GG
Cuts at: 3434 4568 5533 3434
Size: 1134 965 6387
Fragments arranged by size:
6387 1134 965
ScaI AGT'ACT
Cuts at: 8003 8003
Size: 8486
SfcI C'TryA_G
Cuts at: 33 352 1106 2155 2591 3430 4564 5368 5529
Size: 319 754 1049 436 839 1134 804 161
Cuts at: 5529 6214 7207 7398 33
Size: 685 993 191 1121
Fragments arranged by size:
1134 1121 1049 993 839 804 754 685
436 319 191 161
SfiI GGCCn_nnn'nGGCC
Cuts at: 3073 3073
Size: 8486
SfoI GGC'GCC
Cuts at: 4441 5212 5918 6166 4441
Size: 771 706 248 6761
Fragments arranged by size:
6761 771 706 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8486
SgrAI Cr'CCGG_yG
Cuts at: 4436 4436
Size: 8486
SmaI CCC'GGG
Cuts at: 160 1418 5062 5508 160
Size: 1258 3644 446 3138
Fragments arranged by size:
3644 3138 1258 446
SmlI C'TyrA_G
Cuts at: 901 1074 1098 2332 2778 5317 5641 6556 7048
Size: 173 24 1234 446 2539 324 915 492
Cuts at: 7048 7310 901
Size: 262 2077
Fragments arranged by size:
2539 2077 1234 915 492 446 324 262
173 24
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8486
SphI G_CATG'C
Cuts at: 361 3894 5539 6039 361
Size: 3533 1645 500 2808
Fragments arranged by size:
3533 2808 1645 500
SrfI GCCC'GGGC
Cuts at: 5062 5062
Size: 8486
StuI AGG'CCT
Cuts at: 3326 3505 3326
Size: 179 8307
StyI C'CwwG_G
Cuts at: 107 236 887 1580 1802 4262 4677 5620 5974
Size: 129 651 693 222 2460 415 943 354
Cuts at: 5974 107
Size: 2619
Fragments arranged by size:
2619 2460 943 693 651 415 354 222
129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 635 799 1540 2471 2539 4048 4112 4258 5340
Size: 164 741 931 68 1509 64 146 1082
Cuts at: 5340 6069 6202 6661 6870 635
Size: 729 133 459 209 2251
Fragments arranged by size:
2251 1509 1082 931 741 729 459 209
164 146 133 68 64
TatI w'GTAC_w
Cuts at: 3605 4808 5228 5291 8001 3605
Size: 1203 420 63 2710 4090
Fragments arranged by size:
4090 2710 1203 420 63
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 362 394 2296 2328 4522 4554 8003 8035 362
Size: 32 1902 32 2194 32 3449 32 813
Fragments arranged by size:
3449 2194 1902 813 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2887 3591 6283 2887
Size: 704 2692 5090
Fragments arranged by size:
5090 2692 704
XbaI T'CTAG_A
Cuts at: 5517 5517
Size: 8486
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1326 2109 2490 5196 5283 5981 1326
Size: 783 381 2706 87 698 3831
Fragments arranged by size:
3831 2706 783 698 381 87
XhoI C'TCGA_G
Cuts at: 2778 2778
Size: 8486
XmaI C'CCGG_G
Cuts at: 158 1416 5060 5506 158
Size: 1258 3644 446 3138
Fragments arranged by size:
3644 3138 1258 446
XmnI GAAnn'nnTTC
Cuts at: 3052 3230 3052
Size: 178 8308
ZraI GAC'GTC
Cuts at: 2889 2889
Size: 8486
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AloI AscI AvrII BaeI BmtI BsiWI
BsmI BssHII BstZ17I ClaI EcoRV FseI FspAI MfeI
NdeI NheI NotI NruI PacI PshAI PsiI PspXI
PsrI SexAI SnaBI SspI SwaI