Restriction map for
pF1KB0054
(Circular) (Six-base) MAPSORT of: pF1KB0054.seq Check: 7380 from: 1 to: 6221
pF1KB0054 6221 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 881 2319 2606 3259 881
Size: 1438 287 653 3843
Fragments arranged by size:
3843 1438 653 287
Acc65I G'GTAC_C
Cuts at: 3237 3237
Size: 6221
AclI AA'CG_TT
Cuts at: 6061 6061
Size: 6221
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 1028 1617 2156 2242 2692 3067 4046 4478
Size: 517 589 539 86 450 375 979 432
Cuts at: 4478 5225 5493 511
Size: 747 268 1239
Fragments arranged by size:
1239 979 747 589 539 517 450 432
375 268 86
AflII C'TTAA_G
Cuts at: 1450 1450
Size: 6221
AflIII A'CryG_T
Cuts at: 1333 2808 3573 4677 5654 1333
Size: 1475 765 1104 977 1900
Fragments arranged by size:
1900 1475 1104 977 765
AgeI A'CCGG_T
Cuts at: 4609 4609
Size: 6221
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2528 2560 2528
Size: 32 6189
AlwNI CAG_nnn'CTG
Cuts at: 3526 5093 3526
Size: 1567 4654
ApaLI G'TGCA_C
Cuts at: 859 4991 859
Size: 4132 2089
ApoI r'AATT_y
Cuts at: 357 695 1173 1831 1973 3225 5574 5830 357
Size: 338 478 658 142 1252 2349 256 748
Fragments arranged by size:
2349 1252 748 658 478 338 256 142
AseI AT'TA_AT
Cuts at: 20 1349 1682 2474 20
Size: 1329 333 792 3767
Fragments arranged by size:
3767 1329 792 333
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6221
AvaI C'yCGr_G
Cuts at: 2936 3241 2936
Size: 305 5916
BamHI G'GATC_C
Cuts at: 3246 3246
Size: 6221
BanI G'GyrC_C
Cuts at: 3237 3651 3899 3934 3237
Size: 414 248 35 5524
Fragments arranged by size:
5524 414 248 35
BanII G_rGCy'C
Cuts at: 1861 3235 4265 1861
Size: 1374 1030 3817
Fragments arranged by size:
3817 1374 1030
BbeI G_GCGC'C
Cuts at: 3655 3903 3655
Size: 248 5973
BbsI GAAGACnn'nnnn_
Cuts at: 107 1137 3014 107
Size: 1030 1877 3314
Fragments arranged by size:
3314 1877 1030
BciVI GTATCCnnnnn_n'
Cuts at: 2062 4119 4880 6221 2062
Size: 2057 761 1341 2062
Fragments arranged by size:
2062 2057 1341 761
BclI T'GATC_A
Cuts at: 2115 2196 3741 2115
Size: 81 1545 4595
Fragments arranged by size:
4595 1545 81
BfrBI ATG'CAT
Cuts at: 421 1224 421
Size: 803 5418
BglII A'GATC_T
Cuts at: 295 903 3736 5773 295
Size: 608 2833 2037 743
Fragments arranged by size:
2833 2037 743 608
BlpI GC'TnA_GC
Cuts at: 2144 3333 2144
Size: 1189 5032
Bme1580I G_kGCm'C
Cuts at: 831 863 2261 2867 3846 3939 4995 831
Size: 32 1398 606 979 93 1056 2057
Fragments arranged by size:
2057 1398 1056 979 606 93 32
BmgBI CAC'GTC
Cuts at: 2811 2811
Size: 6221
BmrI ACTGGGnnnn_n'
Cuts at: 3433 3514 3849 5993 3433
Size: 81 335 2144 3661
Fragments arranged by size:
3661 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4588 4588
Size: 6221
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 240 2555 240
Size: 2315 3906
Bpu10I CC'TnA_GC
Cuts at: 1855 2964 1855
Size: 1109 5112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2995 3397 4276 4768 5066 2995
Size: 402 879 492 298 4150
Fragments arranged by size:
4150 879 492 402 298
BsaI GGTCTCn'nnnn_
Cuts at: 5925 5925
Size: 6221
BsaAI yAC'GTr
Cuts at: 3574 3574
Size: 6221
BsaBI GATnn'nnATC
Cuts at: 6 3735 5772 6
Size: 3729 2037 455
Fragments arranged by size:
3729 2037 455
BsaHI Gr'CG_yC
Cuts at: 112 3652 3900 4602 6098 112
Size: 3540 248 702 1496 235
Fragments arranged by size:
3540 1496 702 248 235
BsaWI w'CCGG_w
Cuts at: 3410 3931 4609 4883 5030 5441 3410
Size: 521 678 274 147 411 4190
Fragments arranged by size:
4190 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2528 2558 2528
Size: 30 6191
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2375 2554 2375
Size: 179 6042
BseYI C'CCAG_C
Cuts at: 2064 2951 3024 3646 4404 4981 5477 2064
Size: 887 73 622 758 577 496 2808
Fragments arranged by size:
2808 887 758 622 577 496 73
BsiEI CG_ry'CG
Cuts at: 104 3763 3809 5017 104
Size: 3659 46 1208 1308
Fragments arranged by size:
3659 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 863 3235 4013 4203 4995 863
Size: 2372 778 190 792 2089
Fragments arranged by size:
2372 2089 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 433 522 625 433
Size: 89 103 6029
Fragments arranged by size:
6029 103 89
Bsp1286I G_dGCh'C
Cuts at: 831 863 1861 2261 2867 3235 3846 3939 4013
Size: 32 998 400 606 368 611 93 74
Cuts at: 4013 4203 4265 4995 831
Size: 190 62 730 2057
Fragments arranged by size:
2057 998 730 611 606 400 368 190
93 74 62 32
BspEI T'CCGG_A
Cuts at: 5441 5441
Size: 6221
BspHI T'CATG_A
Cuts at: 5397 5397
Size: 6221
BspMI ACCTGCnnnn'nnnn_
Cuts at: 581 3271 3485 3787 4168 581
Size: 2690 214 302 381 2634
Fragments arranged by size:
2690 2634 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3129 4513 4567 6051 6216 50
Size: 3079 1384 54 1484 165 55
Fragments arranged by size:
3079 1484 1384 165 55 54
BsrDI GCAATG_nn'
Cuts at: 421 1488 1944 2063 2499 4133 421
Size: 1067 456 119 436 1634 2509
Fragments arranged by size:
2509 1634 1067 456 436 119
BsrFI r'CCGG_y
Cuts at: 4219 4400 4609 4219
Size: 181 209 5831
Fragments arranged by size:
5831 209 181
BsrGI T'GTAC_A
Cuts at: 371 371
Size: 6221
BssSI C'ACGA_G
Cuts at: 4492 4850 4492
Size: 358 5863
BstAPI GCAn_nnn'nTGC
Cuts at: 1667 3598 1667
Size: 1931 4290
BstBI TT'CG_AA
Cuts at: 355 1242 4582 355
Size: 887 3340 1994
Fragments arranged by size:
3340 1994 887
BstEII G'GTnAC_C
Cuts at: 2585 2585
Size: 6221
BstXI CCAn_nnnn'nTGG
Cuts at: 1969 2877 1969
Size: 908 5313
BstYI r'GATC_y
Cuts at: 1 295 903 3246 3713 3736 4071 4317 5329
Size: 294 608 2343 467 23 335 246 1012
Cuts at: 5329 5415 5427 5773 1
Size: 86 12 346 449
Fragments arranged by size:
2343 1012 608 467 449 346 335 294
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 882 2607 882
Size: 1725 4496
BtgI C'CryG_G
Cuts at: 107 119 2903 107
Size: 12 2784 3425
Fragments arranged by size:
3425 2784 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4171 4352 4515 4171
Size: 181 163 5877
Fragments arranged by size:
5877 181 163
BtsI GCAGTG_nn'
Cuts at: 204 564 860 1005 1330 3464 204
Size: 360 296 145 325 2134 2961
Fragments arranged by size:
2961 2134 360 325 296 145
ClaI AT'CG_AT
Cuts at: 446 2240 446
Size: 1794 4427
DraI TTT'AAA
Cuts at: 3220 3220
Size: 6221
DraIII CAC_nnn'GTG
Cuts at: 855 855
Size: 6221
DrdI GACnn_nn'nnGTC
Cuts at: 3927 4785 3927
Size: 858 5363
EaeI y'GGCC_r
Cuts at: 131 3806 3980 4371 4398 5743 131
Size: 3675 174 391 27 1345 609
Fragments arranged by size:
3675 1345 609 391 174 27
EagI C'GGCC_G
Cuts at: 3806 3806
Size: 6221
EarI CTCTTCn'nnn_
Cuts at: 530 1103 4244 4454 530
Size: 573 3141 210 2297
Fragments arranged by size:
3141 2297 573 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4429 4739 4885 6031 4429
Size: 310 146 1146 4619
Fragments arranged by size:
4619 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3233 3233
Size: 6221
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 240 511 1028 1617 2156 2242 2555 2692 3067
Size: 271 517 589 539 86 313 137 375
Cuts at: 3067 4046 4478 5225 5493 240
Size: 979 432 747 268 968
Fragments arranged by size:
979 968 747 589 539 517 432 375
313 271 268 137 86
EcoNI CCTnn'n_nnAGG
Cuts at: 2357 2357
Size: 6221
EcoO109I rG'GnC_Cy
Cuts at: 2897 3360 2897
Size: 463 5758
EcoRI G'AATT_C
Cuts at: 1973 3225 1973
Size: 1252 4969
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2326 4240 2326
Size: 1914 4307
FspI TGC'GCA
Cuts at: 4002 4002
Size: 6221
HaeII r_GCGC'y
Cuts at: 2015 3655 3903 4925 5904 2015
Size: 1640 248 1022 979 2332
Fragments arranged by size:
2332 1640 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 831 863 1255 1287 2233 2265 2334 2366 4063
Size: 32 392 32 946 32 69 32 1697
Cuts at: 4063 4093 4095 4125 831
Size: 30 2 30 2927
Fragments arranged by size:
2927 1697 946 392 69 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3260 3260
Size: 6221
HindIII A'AGCT_T
Cuts at: 347 509 347
Size: 162 6059
KasI G'GCGC_C
Cuts at: 3651 3899 3651
Size: 248 5973
KpnI G_GTAC'C
Cuts at: 3241 3241
Size: 6221
MfeI C'AATT_G
Cuts at: 401 626 401
Size: 225 5996
MluI A'CGCG_T
Cuts at: 1333 5654 1333
Size: 4321 1900
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1790 4892 5076 1790
Size: 3102 184 2935
Fragments arranged by size:
3102 2935 184
MscI TGG'CCA
Cuts at: 3982 3982
Size: 6221
MslI CAynn'nnrTG
Cuts at: 1967 2264 2875 3016 4210 4337 1967
Size: 297 611 141 1194 127 3851
Fragments arranged by size:
3851 1194 611 297 141 127
MspA1I CmG'CkG
Cuts at: 196 484 1951 2134 2826 3163 3332 3646 4006
Size: 288 1467 183 692 337 169 314 360
Cuts at: 4006 5019 5264 196
Size: 1013 245 1153
Fragments arranged by size:
1467 1153 1013 692 360 337 314 288
245 183 169
NaeI GCC'GGC
Cuts at: 4402 4402
Size: 6221
NarI GG'CG_CC
Cuts at: 3652 3900 3652
Size: 248 5973
NcoI C'CATG_G
Cuts at: 107 2903 107
Size: 2796 3425
NdeI CA'TA_TG
Cuts at: 1704 1704
Size: 6221
NgoMIV G'CCGG_C
Cuts at: 4400 4400
Size: 6221
NsiI A_TGCA'T
Cuts at: 423 1226 423
Size: 803 5418
NspI r_CATG'y
Cuts at: 213 421 614 1818 3274 3774 4681 213
Size: 208 193 1204 1456 500 907 1753
Fragments arranged by size:
1753 1456 1204 907 500 208 193
PasI CC'CwG_GG
Cuts at: 585 2074 585
Size: 1489 4732
PciI A'CATG_T
Cuts at: 4677 4677
Size: 6221
PflMI CCAn_nnn'nTGG
Cuts at: 1867 1939 1867
Size: 72 6149
PmeI GTTT'AAAC
Cuts at: 3220 3220
Size: 6221
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2528 2560 5360 5392 2528
Size: 32 2800 32 3357
Fragments arranged by size:
3357 2800 32 32
PpuMI rG'GwC_Cy
Cuts at: 2897 2897
Size: 6221
PsiI TTA'TAA
Cuts at: 1025 1229 1025
Size: 204 6017
PstI C_TGCA'G
Cuts at: 1193 1211 1499 3268 3953 1193
Size: 18 288 1769 685 3461
Fragments arranged by size:
3461 1769 685 288 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6221
PvuII CAG'CTG
Cuts at: 196 1951 2134 2826 3163 3646 4006 196
Size: 1755 183 692 337 483 360 2411
Fragments arranged by size:
2411 1755 692 483 360 337 183
RsrII CG'GwC_CG
Cuts at: 4416 4416
Size: 6221
SacI G_AGCT'C
Cuts at: 3235 3235
Size: 6221
SalI G'TCGA_C
Cuts at: 3258 3258
Size: 6221
SanDI GG'GwC_CC
Cuts at: 2897 2897
Size: 6221
SapI GCTCTTCn'nnn_
Cuts at: 530 4244 4454 530
Size: 3714 210 2297
Fragments arranged by size:
3714 2297 210
SbfI CC_TGCA'GG
Cuts at: 3268 3268
Size: 6221
ScaI AGT'ACT
Cuts at: 5738 5738
Size: 6221
SfcI C'TryA_G
Cuts at: 33 921 1189 1207 1495 3139 3264 3949 4942
Size: 888 268 18 288 1644 125 685 993
Cuts at: 4942 5133 33
Size: 191 1121
Fragments arranged by size:
1644 1121 993 888 685 288 268 191
125 18
SfoI GGC'GCC
Cuts at: 3653 3901 3653
Size: 248 5973
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6221
SmaI CCC'GGG
Cuts at: 3243 3243
Size: 6221
SmlI C'TyrA_G
Cuts at: 1450 2936 2974 3376 4291 4783 5045 1450
Size: 1486 38 402 915 492 262 2626
Fragments arranged by size:
2626 1486 915 492 402 262 38
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6221
SphI G_CATG'C
Cuts at: 213 1818 3274 3774 213
Size: 1605 1456 500 2660
Fragments arranged by size:
2660 1605 1456 500
SspI AAT'ATT
Cuts at: 1410 2733 1410
Size: 1323 4898
StuI AGG'CCT
Cuts at: 896 896
Size: 6221
StyI C'CwwG_G
Cuts at: 107 473 605 2903 3355 3709 107
Size: 366 132 2298 452 354 2619
Fragments arranged by size:
2619 2298 452 366 354 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1066 3804 3937 4396 4605 1066
Size: 2738 133 459 209 2682
Fragments arranged by size:
2738 2682 459 209 133
TatI w'GTAC_w
Cuts at: 371 504 530 545 1185 2691 5736 371
Size: 133 26 15 640 1506 3045 856
Fragments arranged by size:
3045 1506 856 640 133 26 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2070 2102 5738 5770 2070
Size: 32 3636 32 2521
Fragments arranged by size:
3636 2521 32 32
Tth111I GACn'n_nGTC
Cuts at: 4018 4018
Size: 6221
XbaI T'CTAG_A
Cuts at: 3036 3252 3036
Size: 216 6005
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3716 3716
Size: 6221
XhoI C'TCGA_G
Cuts at: 2936 2936
Size: 6221
XmaI C'CCGG_G
Cuts at: 3241 3241
Size: 6221
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmtI BsgI BsiWI
BsmI BssHII Bsu36I CspCI EcoRV FseI FspAI HpaI
NheI NotI NruI PacI PfoI PmlI PshAI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SwaI XmnI ZraI