Restriction map for
pF1KB0063
(Circular) (Six-base) MAPSORT of: pF1KB0063.seq Check: 8201 from: 1 to: 9716
pF1KB0063 9716 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 124 1102 1150 6427 124
Size: 978 48 5277 3413
Fragments arranged by size:
5277 3413 978 48
AatII G_ACGT'C
Cuts at: 2134 2134
Size: 9716
AccI GT'mk_AC
Cuts at: 1271 4625 6754 1271
Size: 3354 2129 4233
Fragments arranged by size:
4233 3354 2129
Acc65I G'GTAC_C
Cuts at: 6732 6732
Size: 9716
AclI AA'CG_TT
Cuts at: 9556 9556
Size: 9716
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 591 635 2273 2512 2716 3064 3816 6430
Size: 259 44 1638 239 204 348 752 2614
Cuts at: 6430 6663 7541 7973 8720 8988 332
Size: 233 878 432 747 268 1060
Fragments arranged by size:
2614 1638 1060 878 752 747 432 348
268 259 239 233 204 44
AflIII A'CryG_T
Cuts at: 892 2744 2808 3303 7068 8172 9149 892
Size: 1852 64 495 3765 1104 977 1459
Fragments arranged by size:
3765 1852 1459 1104 977 495 64
AgeI A'CCGG_T
Cuts at: 5851 8104 5851
Size: 2253 7463
AhdI GACnn_n'nnGTC
Cuts at: 1654 1918 2130 1654
Size: 264 212 9240
Fragments arranged by size:
9240 264 212
AleI CACnn'nnGTG
Cuts at: 1256 2090 1256
Size: 834 8882
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 545 545
Size: 9716
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2092 2124 2665 2697 6626 6658 2092
Size: 32 541 32 3929 32 5150
Fragments arranged by size:
5150 3929 541 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 457 779 1619 1805 1898 6551 7021 8588 457
Size: 322 840 186 93 4653 470 1567 1585
Fragments arranged by size:
4653 1585 1567 840 470 322 186 93
ApaI G_GGCC'C
Cuts at: 2485 5726 5990 2485
Size: 3241 264 6211
Fragments arranged by size:
6211 3241 264
ApaLI G'TGCA_C
Cuts at: 8486 8486
Size: 9716
ApoI r'AATT_y
Cuts at: 2263 3139 4828 5277 6265 6720 9069 9325 2263
Size: 876 1689 449 988 455 2349 256 2654
Fragments arranged by size:
2654 2349 1689 988 876 455 449 256
AseI AT'TA_AT
Cuts at: 20 4727 20
Size: 4707 5009
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9716
AvaI C'yCGr_G
Cuts at: 1923 2477 4320 4327 4459 5677 5837 6736 1923
Size: 554 1843 7 132 1218 160 899 4903
Fragments arranged by size:
4903 1843 1218 899 554 160 132 7
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 6022 6055 6022
Size: 33 9683
BamHI G'GATC_C
Cuts at: 289 2304 6741 289
Size: 2015 4437 3264
Fragments arranged by size:
4437 3264 2015
BanI G'GyrC_C
Cuts at: 339 1090 5301 6732 7146 7394 7429 339
Size: 751 4211 1431 414 248 35 2626
Fragments arranged by size:
4211 2626 1431 751 414 248 35
BanII G_rGCy'C
Cuts at: 356 671 1046 1325 2485 3782 5242 5726 5737
Size: 315 375 279 1160 1297 1460 484 11
Cuts at: 5737 5947 5990 6323 6730 7760 356
Size: 210 43 333 407 1030 2312
Fragments arranged by size:
2312 1460 1297 1160 1030 484 407 375
333 315 279 210 43 11
BbeI G_GCGC'C
Cuts at: 7150 7398 7150
Size: 248 9468
BbsI GAAGACnn'nnnn_
Cuts at: 396 500 1037 1130 1595 1967 3023 3130 5037
Size: 104 537 93 465 372 1056 107 1907
Cuts at: 5037 6135 396
Size: 1098 3977
Fragments arranged by size:
3977 1907 1098 1056 537 465 372 107
104 93
BbvCI CC'TCA_GC
Cuts at: 2915 3581 4400 6128 2915
Size: 666 819 1728 6503
Fragments arranged by size:
6503 1728 819 666
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 686 720 804 838 910 944 686
Size: 34 84 34 72 34 9458
Fragments arranged by size:
9458 84 72 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7614 8375 9716 7614
Size: 761 1341 7614
Fragments arranged by size:
7614 1341 761
BclI T'GATC_A
Cuts at: 2567 7236 2567
Size: 4669 5047
BglI GCCn_nnn'nGGC
Cuts at: 112 923 1853 2230 5516 112
Size: 811 930 377 3286 4312
Fragments arranged by size:
4312 3286 930 811 377
BglII A'GATC_T
Cuts at: 7231 9268 7231
Size: 2037 7679
BlpI GC'TnA_GC
Cuts at: 475 6828 475
Size: 6353 3363
Bme1580I G_kGCm'C
Cuts at: 872 2485 5304 5345 5726 5990 7341 7434 8490
Size: 1613 2819 41 381 264 1351 93 1056
Cuts at: 8490 872
Size: 2098
Fragments arranged by size:
2819 2098 1613 1351 1056 381 264 93
41
BmgBI CAC'GTC
Cuts at: 2631 2809 2631
Size: 178 9538
BmrI ACTGGGnnnn_n'
Cuts at: 288 859 1406 1793 1886 6928 7009 7344 9488
Size: 571 547 387 93 5042 81 335 2144
Cuts at: 9488 288
Size: 516
Fragments arranged by size:
5042 2144 571 547 516 387 335 93
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8083 8083
Size: 9716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 505 514 535 1325 2173 2215 2921 3653
Size: 174 9 21 790 848 42 706 732
Cuts at: 3653 4564 4879 5316 331
Size: 911 315 437 4731
Fragments arranged by size:
4731 911 848 790 732 706 437 315
174 42 21 9
Bpu10I CC'TnA_GC
Cuts at: 2915 3332 3581 4400 5941 6128 2915
Size: 417 249 819 1541 187 6503
Fragments arranged by size:
6503 1541 819 417 249 187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 1309 3493 4959 6892 7771 8263 8561 166
Size: 1143 2184 1466 1933 879 492 298 1321
Fragments arranged by size:
2184 1933 1466 1321 1143 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2395 2633 2761 3094 4498 4908 5149 6660 9420
Size: 238 128 333 1404 410 241 1511 2760
Cuts at: 9420 2395
Size: 2691
Fragments arranged by size:
2760 2691 1511 1404 410 333 241 238
128
BsaAI yAC'GTr
Cuts at: 2237 7069 2237
Size: 4832 4884
BsaBI GATnn'nnATC
Cuts at: 6 7230 9267 6
Size: 7224 2037 455
Fragments arranged by size:
7224 2037 455
BsaHI Gr'CG_yC
Cuts at: 2131 7147 7395 8097 9593 2131
Size: 5016 248 702 1496 2254
Fragments arranged by size:
5016 2254 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2345 4652 4968 5327 5851 6905 7426 8104 8378
Size: 2307 316 359 524 1054 521 678 274
Cuts at: 8378 8525 8936 2345
Size: 147 411 3125
Fragments arranged by size:
3125 2307 1054 678 524 521 411 359
316 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 985 1015 1171 1201 1312 1342 2665 2695 3605
Size: 30 156 30 111 30 1323 30 910
Cuts at: 3605 3635 985
Size: 30 7066
Fragments arranged by size:
7066 1323 910 156 111 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 502 895 1192 1970 2406 3883 6009 502
Size: 393 297 778 436 1477 2126 4209
Fragments arranged by size:
4209 2126 1477 778 436 393 297
BseYI C'CCAG_C
Cuts at: 355 772 2524 2757 3365 3638 3911 4155 4184
Size: 417 1752 233 608 273 273 244 29
Cuts at: 4184 4645 7141 7899 8476 8972 355
Size: 461 2496 758 577 496 1099
Fragments arranged by size:
2496 1752 1099 758 608 577 496 461
417 273 273 244 233 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1709 2231 2440 3214 3451 3724 4069 4270 1709
Size: 522 209 774 237 273 345 201 7155
Fragments arranged by size:
7155 774 522 345 273 237 209 201
BsiEI CG_ry'CG
Cuts at: 104 597 1071 1542 7258 7304 8512 104
Size: 493 474 471 5716 46 1208 1308
Fragments arranged by size:
5716 1308 1208 493 474 471 46
BsiHKAI G_wGCw'C
Cuts at: 377 887 1046 1325 3416 3575 4235 5737 6323
Size: 510 159 279 2091 159 660 1502 586
Cuts at: 6323 6519 6730 7508 7698 8490 377
Size: 196 211 778 190 792 1603
Fragments arranged by size:
2091 1603 1502 792 778 660 586 510
279 211 196 190 159 159
BsmI GAATG_Cn'
Cuts at: 2210 4773 2210
Size: 2563 7153
BsmBI CGTCTCn'nnnn_
Cuts at: 1926 2011 6183 1926
Size: 85 4172 5459
Fragments arranged by size:
5459 4172 85
Bsp1286I G_dGCh'C
Cuts at: 356 377 671 872 887 1046 1325 2485 3416
Size: 21 294 201 15 159 279 1160 931
Cuts at: 3416 3575 3782 4235 5242 5304 5345 5726 5737
Size: 159 207 453 1007 62 41 381 11
Cuts at: 5737 5947 5990 6323 6519 6730 7341 7434 7508
Size: 210 43 333 196 211 611 93 74
Cuts at: 7508 7698 7760 8490 356
Size: 190 62 730 1582
Fragments arranged by size:
1582 1160 1007 931 730 611 453 381
333 294 279 211 210 207 201 196
190 159 159 93 74 62 62 43
41 21 15 11
BspEI T'CCGG_A
Cuts at: 2345 4652 5327 8936 2345
Size: 2307 675 3609 3125
Fragments arranged by size:
3609 3125 2307 675
BspHI T'CATG_A
Cuts at: 8892 8892
Size: 9716
BspMI ACCTGCnnnn'nnnn_
Cuts at: 124 1102 1150 2253 3779 5867 6341 6427 6766
Size: 978 48 1103 1526 2088 474 86 339
Cuts at: 6766 6980 7282 7663 124
Size: 214 302 381 2177
Fragments arranged by size:
2177 2088 1526 1103 978 474 381 339
302 214 86 48
BsrBI CCG'CTC
Cuts at: 50 8008 8062 9546 9711 50
Size: 7958 54 1484 165 55
Fragments arranged by size:
7958 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 529 789 2315 5573 6074 7628 529
Size: 260 1526 3258 501 1554 2617
Fragments arranged by size:
3258 2617 1554 1526 501 260
BsrFI r'CCGG_y
Cuts at: 701 1169 1210 1675 2411 5851 7714 7895 8104
Size: 468 41 465 736 3440 1863 181 209
Cuts at: 8104 701
Size: 2313
Fragments arranged by size:
3440 2313 1863 736 468 465 209 181
41
BsrGI T'GTAC_A
Cuts at: 2072 2072
Size: 9716
BssSI C'ACGA_G
Cuts at: 1849 2086 7987 8345 1849
Size: 237 5901 358 3220
Fragments arranged by size:
5901 3220 358 237
BstAPI GCAn_nnn'nTGC
Cuts at: 1778 6238 7093 1778
Size: 4460 855 4401
Fragments arranged by size:
4460 4401 855
BstBI TT'CG_AA
Cuts at: 824 8077 824
Size: 7253 2463
BstEII G'GTnAC_C
Cuts at: 2499 5854 2499
Size: 3355 6361
BstXI CCAn_nnnn'nTGG
Cuts at: 2092 2514 3060 3447 5502 2092
Size: 422 546 387 2055 6306
Fragments arranged by size:
6306 2055 546 422 387
BstYI r'GATC_y
Cuts at: 1 289 306 2304 4143 4496 5969 6466 6741
Size: 288 17 1998 1839 353 1473 497 275
Cuts at: 6741 7208 7231 7566 7812 8824 8910 8922 9268
Size: 467 23 335 246 1012 86 12 346
Cuts at: 9268 1
Size: 449
Fragments arranged by size:
1998 1839 1473 1012 497 467 449 353
346 335 288 275 246 86 23 17
12
Bsu36I CC'TnA_GG
Cuts at: 514 2389 4054 4303 5992 514
Size: 1875 1665 249 1689 4238
Fragments arranged by size:
4238 1875 1689 1665 249
BtgI C'CryG_G
Cuts at: 107 978 1350 1716 3813 4037 5234 5366 5687
Size: 871 372 366 2097 224 1197 132 321
Cuts at: 5687 6342 6446 107
Size: 655 104 3377
Fragments arranged by size:
3377 2097 1197 871 655 372 366 321
224 132 104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 397 950 5430 7666 7847 8010 397
Size: 553 4480 2236 181 163 2103
Fragments arranged by size:
4480 2236 2103 553 181 163
BtsI GCAGTG_nn'
Cuts at: 724 882 1044 1192 1350 1378 1509 1750 2558
Size: 158 162 148 158 28 131 241 808
Cuts at: 2558 5744 6959 724
Size: 3186 1215 3481
Fragments arranged by size:
3481 3186 1215 808 241 162 158 158
148 131 28
ClaI AT'CG_AT
Cuts at: 2597 2654 2597
Size: 57 9659
DraI TTT'AAA
Cuts at: 6715 6715
Size: 9716
DraIII CAC_nnn'GTG
Cuts at: 2463 2463
Size: 9716
DrdI GACnn_nn'nnGTC
Cuts at: 5593 7422 8280 5593
Size: 1829 858 7029
Fragments arranged by size:
7029 1829 858
EaeI y'GGCC_r
Cuts at: 1260 1347 1353 1539 1719 1812 1833 1875 2231
Size: 87 6 186 180 93 21 42 356
Cuts at: 2231 3134 3626 5555 7301 7475 7866 7893 9238
Size: 903 492 1929 1746 174 391 27 1345
Cuts at: 9238 1260
Size: 1738
Fragments arranged by size:
1929 1746 1738 1345 903 492 391 356
186 180 174 93 87 42 27 21
6
EagI C'GGCC_G
Cuts at: 1539 7301 1539
Size: 5762 3954
EarI CTCTTCn'nnn_
Cuts at: 230 1225 2009 2200 2488 3992 5725 7739 7949
Size: 995 784 191 288 1504 1733 2014 210
Cuts at: 7949 230
Size: 1997
Fragments arranged by size:
2014 1997 1733 1504 995 784 288 210
191
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 7924 8234 8380 9526 7924
Size: 310 146 1146 8114
Fragments arranged by size:
8114 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1044 1323 5735 6321 6728 1044
Size: 279 4412 586 407 4032
Fragments arranged by size:
4412 4032 586 407 279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 332 505 514 535 591 635 1325 2173
Size: 1 173 9 21 56 44 690 848
Cuts at: 2173 2215 2273 2512 2716 2921 3064 3653 3816
Size: 42 58 239 204 205 143 589 163
Cuts at: 3816 4564 4879 5316 6430 6663 7541 7973 8720
Size: 748 315 437 1114 233 878 432 747
Cuts at: 8720 8988 331
Size: 268 1059
Fragments arranged by size:
1114 1059 878 848 748 747 690 589
437 432 315 268 239 233 205 204
173 163 143 58 56 44 42 21
9 1
EcoNI CCTnn'n_nnAGG
Cuts at: 560 5480 5556 560
Size: 4920 76 4720
Fragments arranged by size:
4920 4720 76
EcoO109I rG'GnC_Cy
Cuts at: 177 581 652 1632 2481 2482 3075 3677 4689
Size: 404 71 980 849 1 593 602 1012
Cuts at: 4689 5722 5723 5987 6855 177
Size: 1033 1 264 868 3038
Fragments arranged by size:
3038 1033 1012 980 868 849 602 593
404 264 71 1 1
EcoRI G'AATT_C
Cuts at: 4828 6265 6720 4828
Size: 1437 455 7824
Fragments arranged by size:
7824 1437 455
EcoRV GAT'ATC
Cuts at: 2450 2450
Size: 9716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 7735 7735
Size: 9716
FspI TGC'GCA
Cuts at: 7497 7497
Size: 9716
HaeII r_GCGC'y
Cuts at: 2225 6136 7150 7398 8420 9399 2225
Size: 3911 1014 248 1022 979 2542
Fragments arranged by size:
3911 2542 1022 1014 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 298 330 800 832 1640 1672 1904 1936 2116
Size: 32 470 32 808 32 232 32 180
Cuts at: 2116 2148 4340 4372 4455 4487 5497 5529 5788
Size: 32 2192 32 83 32 1010 32 259
Cuts at: 5788 5820 5905 5937 6073 6105 6142 6174 7558
Size: 32 85 32 136 32 37 32 1384
Cuts at: 7558 7588 7590 7620 298
Size: 30 2 30 2394
Fragments arranged by size:
2394 2192 1384 1010 808 470 259 232
180 136 85 83 37 32 32 32
32 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2025 4260 4977 6564 6609 6755 2025
Size: 2235 717 1587 45 146 4986
Fragments arranged by size:
4986 2235 1587 717 146 45
HindIII A'AGCT_T
Cuts at: 363 363
Size: 9716
HpaI GTT'AAC
Cuts at: 6609 6609
Size: 9716
KasI G'GCGC_C
Cuts at: 7146 7394 7146
Size: 248 9468
KpnI G_GTAC'C
Cuts at: 6736 6736
Size: 9716
MfeI C'AATT_G
Cuts at: 966 6026 966
Size: 5060 4656
MluI A'CGCG_T
Cuts at: 9149 9149
Size: 9716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3041 4745 5786 8387 8571 3041
Size: 1704 1041 2601 184 4186
Fragments arranged by size:
4186 2601 1704 1041 184
MscI TGG'CCA
Cuts at: 1349 1814 1877 2233 3136 5557 7477 1349
Size: 465 63 356 903 2421 1920 3588
Fragments arranged by size:
3588 2421 1920 903 465 356 63
MslI CAynn'nnrTG
Cuts at: 1256 2090 3427 3700 4246 5449 7705 7832 1256
Size: 834 1337 273 546 1203 2256 127 3140
Fragments arranged by size:
3140 2256 1337 1203 834 546 273 127
MspA1I CmG'CkG
Cuts at: 126 762 1079 1265 1358 1451 1544 1823 1931
Size: 636 317 186 93 93 93 279 108
Cuts at: 1931 1955 3397 3815 4155 4216 5032 5294 6827
Size: 24 1442 418 340 61 816 262 1533
Cuts at: 6827 7141 7501 8514 8759 126
Size: 314 360 1013 245 1083
Fragments arranged by size:
1533 1442 1083 1013 816 636 418 360
340 317 314 279 262 245 186 108
93 93 93 61 24
NaeI GCC'GGC
Cuts at: 7897 7897
Size: 9716
NarI GG'CG_CC
Cuts at: 7147 7395 7147
Size: 248 9468
NcoI C'CATG_G
Cuts at: 107 1350 1716 4037 5234 5366 6342 6446 107
Size: 1243 366 2321 1197 132 976 104 3377
Fragments arranged by size:
3377 2321 1243 1197 976 366 132 104
NdeI CA'TA_TG
Cuts at: 6234 6234
Size: 9716
NgoMIV G'CCGG_C
Cuts at: 7895 7895
Size: 9716
NspI r_CATG'y
Cuts at: 896 1153 1560 2748 3307 6769 7269 8176 896
Size: 257 407 1188 559 3462 500 907 2436
Fragments arranged by size:
3462 2436 1188 907 559 500 407 257
PasI CC'CwG_GG
Cuts at: 1164 1613 6306 1164
Size: 449 4693 4574
Fragments arranged by size:
4693 4574 449
PciI A'CATG_T
Cuts at: 892 2744 3303 8172 892
Size: 1852 559 4869 2436
Fragments arranged by size:
4869 2436 1852 559
PflMI CCAn_nnn'nTGG
Cuts at: 249 3186 249
Size: 2937 6779
PfoI T'CCnGG_A
Cuts at: 2144 2192 3977 4477 4750 5023 5644 2144
Size: 48 1785 500 273 273 621 6216
Fragments arranged by size:
6216 1785 621 500 273 273 48
PmeI GTTT'AAAC
Cuts at: 6715 6715
Size: 9716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2665 2697 5376 5408 8855 8887 2665
Size: 32 2679 32 3447 32 3494
Fragments arranged by size:
3494 3447 2679 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 177 3075 177
Size: 2898 6818
PshAI GACnn'nnGTC
Cuts at: 3759 3759
Size: 9716
PspOMI G'GGCC_C
Cuts at: 2481 5722 5986 2481
Size: 3241 264 6211
Fragments arranged by size:
6211 3241 264
PspXI vC'TCGA_Gb
Cuts at: 4327 5677 4327
Size: 1350 8366
PstI C_TGCA'G
Cuts at: 534 757 850 877 1039 1225 1345 1411 1504
Size: 223 93 27 162 186 120 66 93
Cuts at: 1504 1624 3099 6763 7448 534
Size: 120 1475 3664 685 2802
Fragments arranged by size:
3664 2802 1475 685 223 186 162 120
120 93 93 66 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9716
PvuII CAG'CTG
Cuts at: 126 762 3397 4155 4216 5032 5294 7141 7501
Size: 636 2635 758 61 816 262 1847 360
Cuts at: 7501 126
Size: 2341
Fragments arranged by size:
2635 2341 1847 816 758 636 360 262
61
RsrII CG'GwC_CG
Cuts at: 7911 7911
Size: 9716
SacI G_AGCT'C
Cuts at: 1046 1325 5737 6323 6730 1046
Size: 279 4412 586 407 4032
Fragments arranged by size:
4412 4032 586 407 279
SacII CC_GC'GG
Cuts at: 3816 3816
Size: 9716
SalI G'TCGA_C
Cuts at: 6753 6753
Size: 9716
SapI GCTCTTCn'nnn_
Cuts at: 2200 5725 7739 7949 2200
Size: 3525 2014 210 3967
Fragments arranged by size:
3967 3525 2014 210
SbfI CC_TGCA'GG
Cuts at: 6763 6763
Size: 9716
ScaI AGT'ACT
Cuts at: 2170 2680 3688 4391 4611 9233 2170
Size: 510 1008 703 220 4622 2653
Fragments arranged by size:
4622 2653 1008 703 510 220
SexAI A'CCwGG_T
Cuts at: 500 2572 3989 500
Size: 2072 1417 6227
Fragments arranged by size:
6227 2072 1417
SfcI C'TryA_G
Cuts at: 33 530 588 753 846 873 1035 1221 1341
Size: 497 58 165 93 27 162 186 120
Cuts at: 1341 1407 1500 1620 1950 3095 3558 4124 4377
Size: 66 93 120 330 1145 463 566 253
Cuts at: 4377 5219 5925 6759 7444 8437 8628 33
Size: 842 706 834 685 993 191 1121
Fragments arranged by size:
1145 1121 993 842 834 706 685 566
497 463 330 253 191 186 165 162
120 120 93 93 66 58 27
SfiI GGCCn_nnn'nGGCC
Cuts at: 5516 5516
Size: 9716
SfoI GGC'GCC
Cuts at: 7148 7396 7148
Size: 248 9468
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9716
SmaI CCC'GGG
Cuts at: 2479 4322 5839 6738 2479
Size: 1843 1517 899 5457
Fragments arranged by size:
5457 1843 1517 899
SmlI C'TyrA_G
Cuts at: 181 1324 3508 4327 4938 5677 6871 7786 8278
Size: 1143 2184 819 611 739 1194 915 492
Cuts at: 8278 8540 181
Size: 262 1357
Fragments arranged by size:
2184 1357 1194 1143 915 819 739 611
492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9716
SphI G_CATG'C
Cuts at: 6769 7269 6769
Size: 500 9216
StuI AGG'CCT
Cuts at: 1865 2258 2783 3233 3406 3586 4052 4405 4871
Size: 393 525 450 173 180 466 353 466
Cuts at: 4871 1865
Size: 6710
Fragments arranged by size:
6710 525 466 466 450 393 353 180
173
StyI C'CwwG_G
Cuts at: 107 1350 1716 4037 4917 5234 5366 5552 6050
Size: 1243 366 2321 880 317 132 186 498
Cuts at: 6050 6342 6446 6850 7204 107
Size: 292 104 404 354 2619
Fragments arranged by size:
2619 2321 1243 880 498 404 366 354
317 292 186 132 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1177 2098 3174 3916 4930 5319 5686 5968 7299
Size: 921 1076 742 1014 389 367 282 1331
Cuts at: 7299 7432 7891 8100 1177
Size: 133 459 209 2793
Fragments arranged by size:
2793 1331 1076 1014 921 742 459 389
367 282 209 133
TatI w'GTAC_w
Cuts at: 526 2072 2168 2678 3251 3686 4389 4609 9231
Size: 1546 96 510 573 435 703 220 4622
Cuts at: 9231 526
Size: 1011
Fragments arranged by size:
4622 1546 1011 703 573 510 435 220
96
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 974 985 1006 1017 1171 1203 1312 1344 1636
Size: 11 21 11 154 32 109 32 292
Cuts at: 1636 1668 1822 1854 2284 2316 3605 3637 6164
Size: 32 154 32 430 32 1289 32 2527
Cuts at: 6164 6196 6423 6455 9233 9265 974
Size: 32 227 32 2778 32 1425
Fragments arranged by size:
2778 2527 1425 1289 430 292 227 154
154 109 32 32 32 32 32 32
32 32 32 21 11 11
Tth111I GACn'n_nGTC
Cuts at: 7513 7513
Size: 9716
XbaI T'CTAG_A
Cuts at: 2310 6747 2310
Size: 4437 5279
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 567 2511 4539 5313 5373 5580 5980 7211 567
Size: 1944 2028 774 60 207 400 1231 3072
Fragments arranged by size:
3072 2028 1944 1231 774 400 207 60
XhoI C'TCGA_G
Cuts at: 4327 5677 4327
Size: 1350 8366
XmaI C'CCGG_G
Cuts at: 2477 4320 5837 6736 2477
Size: 1843 1517 899 5457
Fragments arranged by size:
5457 1843 1517 899
XmnI GAAnn'nnTTC
Cuts at: 365 365
Size: 9716
ZraI GAC'GTC
Cuts at: 2132 2132
Size: 9716
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AflII AscI AvrII BfrBI BmtI BsiWI BssHII
BstZ17I CspCI FseI FspAI NheI NotI NruI NsiI
PacI PmlI PsiI PsrI SanDI SgrAI SnaBI SrfI
SspI SwaI