Restriction map for
pF1KB0065
(Circular) (Six-base) MAPSORT of: pF1KB0065.seq Check: 1271 from: 1 to: 9236
pF1KB0065 9236 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2922 5838 2922
Size: 2916 6320
AatII G_ACGT'C
Cuts at: 1535 5935 6111 1535
Size: 4400 176 4660
Fragments arranged by size:
4660 4400 176
AccI GT'mk_AC
Cuts at: 3289 6274 3289
Size: 2985 6251
Acc65I G'GTAC_C
Cuts at: 6252 6252
Size: 9236
AclI AA'CG_TT
Cuts at: 9076 9076
Size: 9236
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 225 235 244 290 1298 1399 1664 1872 2440
Size: 10 9 46 1008 101 265 208 568
Cuts at: 2440 2553 3882 3998 4774 4805 6000 6119 7061
Size: 113 1329 116 776 31 1195 119 942
Cuts at: 7061 7493 8240 8508 225
Size: 432 747 268 953
Fragments arranged by size:
1329 1195 1008 953 942 776 747 568
432 268 265 208 119 116 113 101
46 31 10 9
AfeI AGC'GCT
Cuts at: 154 154
Size: 9236
AflIII A'CryG_T
Cuts at: 3672 6588 7692 8669 3672
Size: 2916 1104 977 4239
Fragments arranged by size:
4239 2916 1104 977
AgeI A'CCGG_T
Cuts at: 7624 7624
Size: 9236
AhdI GACnn_n'nnGTC
Cuts at: 4678 4678
Size: 9236
AleI CACnn'nnGTG
Cuts at: 2534 2956 2534
Size: 422 8814
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 5870 5915 5870
Size: 45 9191
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 590 622 590
Size: 32 9204
AlwNI CAG_nnn'CTG
Cuts at: 252 279 430 2440 4695 4828 4869 6097 6541
Size: 27 151 2010 2255 133 41 1228 444
Cuts at: 6541 8108 252
Size: 1567 1380
Fragments arranged by size:
2255 2010 1567 1380 1228 444 151 133
41 27
ApaI G_GGCC'C
Cuts at: 138 5626 138
Size: 5488 3748
ApaLI G'TGCA_C
Cuts at: 8006 8006
Size: 9236
ApoI r'AATT_y
Cuts at: 469 668 2769 6240 8589 8845 469
Size: 199 2101 3471 2349 256 860
Fragments arranged by size:
3471 2349 2101 860 256 199
AseI AT'TA_AT
Cuts at: 20 446 3134 3278 5425 20
Size: 426 2688 144 2147 3831
Fragments arranged by size:
3831 2688 2147 426 144
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9236
AvaI C'yCGr_G
Cuts at: 6256 6256
Size: 9236
AvrII C'CTAG_G
Cuts at: 3159 5313 3159
Size: 2154 7082
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5336 5369 5336
Size: 33 9203
BamHI G'GATC_C
Cuts at: 2121 2262 5530 6261 2121
Size: 141 3268 731 5096
Fragments arranged by size:
5096 3268 731 141
BanI G'GyrC_C
Cuts at: 1114 2307 3226 3948 5062 6252 6666 6914 6949
Size: 1193 919 722 1114 1190 414 248 35
Cuts at: 6949 1114
Size: 3401
Fragments arranged by size:
3401 1193 1190 1114 919 722 414 248
35
BanII G_rGCy'C
Cuts at: 138 235 527 1517 3043 3067 5626 6250 7280
Size: 97 292 990 1526 24 2559 624 1030
Cuts at: 7280 138
Size: 2094
Fragments arranged by size:
2559 2094 1526 1030 990 624 292 97
24
BbeI G_GCGC'C
Cuts at: 6670 6918 6670
Size: 248 8988
BbsI GAAGACnn'nnnn_
Cuts at: 203 1535 3339 4224 4762 5121 5406 5447 6107
Size: 1332 1804 885 538 359 285 41 660
Cuts at: 6107 203
Size: 3332
Fragments arranged by size:
3332 1804 1332 885 660 538 359 285
41
BbvCI CC'TCA_GC
Cuts at: 785 785
Size: 9236
BciVI GTATCCnnnnn_n'
Cuts at: 1667 7134 7895 9236 1667
Size: 5467 761 1341 1667
Fragments arranged by size:
5467 1667 1341 761
BclI T'GATC_A
Cuts at: 474 663 696 978 4038 6756 474
Size: 189 33 282 3060 2718 2954
Fragments arranged by size:
3060 2954 2718 282 189 33
BfrBI ATG'CAT
Cuts at: 5575 5827 5575
Size: 252 8984
BglI GCCn_nnn'nGGC
Cuts at: 3946 3946
Size: 9236
BglII A'GATC_T
Cuts at: 1032 2085 5135 6751 8788 1032
Size: 1053 3050 1616 2037 1480
Fragments arranged by size:
3050 2037 1616 1480 1053
BlpI GC'TnA_GC
Cuts at: 2459 3634 6348 2459
Size: 1175 2714 5347
Fragments arranged by size:
5347 2714 1175
Bme1580I G_kGCm'C
Cuts at: 138 2596 4001 4371 5626 6861 6954 8010 138
Size: 2458 1405 370 1255 1235 93 1056 1364
Fragments arranged by size:
2458 1405 1364 1255 1235 1056 370 93
BmgBI CAC'GTC
Cuts at: 5868 5868
Size: 9236
BmrI ACTGGGnnnn_n'
Cuts at: 3947 4857 5647 5933 6448 6529 6864 9008 3947
Size: 910 790 286 515 81 335 2144 4175
Fragments arranged by size:
4175 2144 910 790 515 335 286 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7603 7603
Size: 9236
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 563 986 2236 2827 3591 3740 4186 4579 5147
Size: 423 1250 591 764 149 446 393 568
Cuts at: 5147 5369 5675 5735 5879 563
Size: 222 306 60 144 3920
Fragments arranged by size:
3920 1250 764 591 568 446 423 393
306 222 149 144 60
Bpu10I CC'TnA_GC
Cuts at: 785 2501 5029 6009 785
Size: 1716 2528 980 4012
Fragments arranged by size:
4012 2528 1716 980
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2419 2657 5181 6412 7291 7783 8081 2419
Size: 238 2524 1231 879 492 298 3574
Fragments arranged by size:
3574 2524 1231 879 492 298 238
BsaI GGTCTCn'nnnn_
Cuts at: 998 1376 5408 8940 998
Size: 378 4032 3532 1294
Fragments arranged by size:
4032 3532 1294 378
BsaAI yAC'GTr
Cuts at: 2082 6589 2082
Size: 4507 4729
BsaBI GATnn'nnATC
Cuts at: 6 6750 8787 6
Size: 6744 2037 455
Fragments arranged by size:
6744 2037 455
BsaHI Gr'CG_yC
Cuts at: 1532 5206 5932 6108 6667 6915 7617 9113 1532
Size: 3674 726 176 559 248 702 1496 1655
Fragments arranged by size:
3674 1655 1496 726 702 559 248 176
BsaWI w'CCGG_w
Cuts at: 852 4899 6425 6946 7624 7898 8045 8456 852
Size: 4047 1526 521 678 274 147 411 1632
Fragments arranged by size:
4047 1632 1526 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 5251 5281 5342 5372 5852 5882 5251
Size: 30 61 30 480 30 8605
Fragments arranged by size:
8605 480 61 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 517 602 983 1152 1503 2738 5272 5672 5876
Size: 85 381 169 351 1235 2534 400 204
Cuts at: 5876 5957 517
Size: 81 3796
Fragments arranged by size:
3796 2534 1235 400 381 351 204 169
85 81
BseYI C'CCAG_C
Cuts at: 351 594 4201 5022 5058 6661 7419 7996 8492
Size: 243 3607 821 36 1603 758 577 496
Cuts at: 8492 351
Size: 1095
Fragments arranged by size:
3607 1603 1095 821 758 577 496 243
36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3020 3856 4011 4853 3020
Size: 836 155 842 7403
Fragments arranged by size:
7403 842 836 155
BsiEI CG_ry'CG
Cuts at: 104 6778 6824 8032 104
Size: 6674 46 1208 1308
Fragments arranged by size:
6674 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 527 1475 1517 2430 3067 6250 7028 7218 8010
Size: 948 42 913 637 3183 778 190 792
Cuts at: 8010 527
Size: 1753
Fragments arranged by size:
3183 1753 948 913 792 778 637 190
42
BsmI GAATG_Cn'
Cuts at: 2198 4350 5577 2198
Size: 2152 1227 5857
Fragments arranged by size:
5857 2152 1227
BsmBI CGTCTCn'nnnn_
Cuts at: 1889 5858 1889
Size: 3969 5267
Bsp1286I G_dGCh'C
Cuts at: 138 235 527 1475 1517 2430 2596 3043 3067
Size: 97 292 948 42 913 166 447 24
Cuts at: 3067 4001 4371 5626 6250 6861 6954 7028 7218
Size: 934 370 1255 624 611 93 74 190
Cuts at: 7218 7280 8010 138
Size: 62 730 1364
Fragments arranged by size:
1364 1255 948 934 913 730 624 611
447 370 292 190 166 97 93 74
62 42 24
BspEI T'CCGG_A
Cuts at: 8456 8456
Size: 9236
BspHI T'CATG_A
Cuts at: 8412 8412
Size: 9236
BspMI ACCTGCnnnn'nnnn_
Cuts at: 465 2922 3059 4972 5838 6286 6500 6802 7183
Size: 2457 137 1913 866 448 214 302 381
Cuts at: 7183 465
Size: 2518
Fragments arranged by size:
2518 2457 1913 866 448 381 302 214
137
BsrBI CCG'CTC
Cuts at: 50 7528 7582 9066 9231 50
Size: 7478 54 1484 165 55
Fragments arranged by size:
7478 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 778 1084 1277 2318 3645 5705 6131 7148 778
Size: 306 193 1041 1327 2060 426 1017 2866
Fragments arranged by size:
2866 2060 1327 1041 1017 426 306 193
BsrFI r'CCGG_y
Cuts at: 7234 7415 7624 7234
Size: 181 209 8846
Fragments arranged by size:
8846 209 181
BsrGI T'GTAC_A
Cuts at: 270 270
Size: 9236
BssSI C'ACGA_G
Cuts at: 3317 7507 7865 3317
Size: 4190 358 4688
Fragments arranged by size:
4688 4190 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1114 1141 4831 4945 6613 1114
Size: 27 3690 114 1668 3737
Fragments arranged by size:
3737 3690 1668 114 27
BstBI TT'CG_AA
Cuts at: 3207 7597 3207
Size: 4390 4846
BstEII G'GTnAC_C
Cuts at: 2350 4723 2350
Size: 2373 6863
BstXI CCAn_nnnn'nTGG
Cuts at: 643 1146 3561 643
Size: 503 2415 6318
Fragments arranged by size:
6318 2415 503
BstYI r'GATC_y
Cuts at: 1 898 1032 2085 2121 2262 5135 5530 6261
Size: 897 134 1053 36 141 2873 395 731
Cuts at: 6261 6728 6751 7086 7332 8344 8430 8442 8788
Size: 467 23 335 246 1012 86 12 346
Cuts at: 8788 1
Size: 449
Fragments arranged by size:
2873 1053 1012 897 731 467 449 395
346 335 246 141 134 86 36 23
12
Bsu36I CC'TnA_GG
Cuts at: 1072 2753 3591 1072
Size: 1681 838 6717
Fragments arranged by size:
6717 1681 838
BtgI C'CryG_G
Cuts at: 4661 4661
Size: 9236
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2149 5634 7186 7367 7530 2149
Size: 3485 1552 181 163 3855
Fragments arranged by size:
3855 3485 1552 181 163
BtsI GCAGTG_nn'
Cuts at: 633 1005 1056 1139 1353 3999 4418 6479 633
Size: 372 51 83 214 2646 419 2061 3390
Fragments arranged by size:
3390 2646 2061 419 372 214 83 51
DraI TTT'AAA
Cuts at: 4542 6235 4542
Size: 1693 7543
DraIII CAC_nnn'GTG
Cuts at: 4618 5677 4618
Size: 1059 8177
DrdI GACnn_nn'nnGTC
Cuts at: 6942 7800 6942
Size: 858 8378
EaeI y'GGCC_r
Cuts at: 2180 3243 4424 6821 6995 7386 7413 8758 2180
Size: 1063 1181 2397 174 391 27 1345 2658
Fragments arranged by size:
2658 2397 1345 1181 1063 391 174 27
EagI C'GGCC_G
Cuts at: 6821 6821
Size: 9236
EarI CTCTTCn'nnn_
Cuts at: 244 1891 2156 2572 2696 3278 3386 3420 3488
Size: 1647 265 416 124 582 108 34 68
Cuts at: 3488 7259 7469 244
Size: 3771 210 2011
Fragments arranged by size:
3771 2011 1647 582 416 265 210 124
108 68 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 7444 7754 7900 9046 7444
Size: 310 146 1146 7634
Fragments arranged by size:
7634 1146 310 146
EcoICRI GAG'CTC
Cuts at: 525 1515 3065 6248 525
Size: 990 1550 3183 3513
Fragments arranged by size:
3513 3183 1550 990
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 225 235 244 290 563 986 1298 1399 1664
Size: 10 9 46 273 423 312 101 265
Cuts at: 1664 1872 2236 2440 2553 2827 3591 3740 3882
Size: 208 364 204 113 274 764 149 142
Cuts at: 3882 3998 4186 4579 4774 4805 5147 5369 5675
Size: 116 188 393 195 31 342 222 306
Cuts at: 5675 5735 5879 6000 6119 7061 7493 8240 8508
Size: 60 144 121 119 942 432 747 268
Cuts at: 8508 225
Size: 953
Fragments arranged by size:
953 942 764 747 432 423 393 364
342 312 306 274 273 268 265 222
208 204 195 188 149 144 142 121
119 116 113 101 60 46 31 10
9
EcoO109I rG'GnC_Cy
Cuts at: 135 427 1861 2878 5244 5622 5623 5962 6375
Size: 292 1434 1017 2366 378 1 339 413
Cuts at: 6375 135
Size: 2996
Fragments arranged by size:
2996 2366 1434 1017 413 378 339 292
1
EcoRI G'AATT_C
Cuts at: 6240 6240
Size: 9236
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3912 7255 3912
Size: 3343 5893
FspI TGC'GCA
Cuts at: 7017 7017
Size: 9236
HaeII r_GCGC'y
Cuts at: 156 6670 6918 7940 8919 156
Size: 6514 248 1022 979 473
Fragments arranged by size:
6514 1022 979 473 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 351 383 902 934 1504 1536 1594 1626 2106
Size: 32 519 32 570 32 58 32 480
Cuts at: 2106 2138 2955 2987 3326 3358 3612 3644 3713
Size: 32 817 32 339 32 254 32 69
Cuts at: 3713 3745 4299 4331 4664 4696 5120 5152 7078
Size: 32 554 32 333 32 424 32 1926
Cuts at: 7078 7108 7110 7140 351
Size: 30 2 30 2447
Fragments arranged by size:
2447 1926 817 570 554 519 480 424
339 333 254 69 58 32 32 32
32 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2379 5811 6275 2379
Size: 3432 464 5340
Fragments arranged by size:
5340 3432 464
HindIII A'AGCT_T
Cuts at: 3405 3405
Size: 9236
HpaI GTT'AAC
Cuts at: 2379 5811 2379
Size: 3432 5804
KasI G'GCGC_C
Cuts at: 6666 6914 6666
Size: 248 8988
KpnI G_GTAC'C
Cuts at: 6256 6256
Size: 9236
MfeI C'AATT_G
Cuts at: 2267 3540 2267
Size: 1273 7963
MluI A'CGCG_T
Cuts at: 8669 8669
Size: 9236
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 92 1848 4662 6006 7907 8091 92
Size: 1756 2814 1344 1901 184 1237
Fragments arranged by size:
2814 1901 1756 1344 1237 184
MscI TGG'CCA
Cuts at: 2182 6997 2182
Size: 4815 4421
MslI CAynn'nnrTG
Cuts at: 446 641 1946 2534 2872 2956 3224 4343 5842
Size: 195 1305 588 338 84 268 1119 1499
Cuts at: 5842 6217 7225 7352 446
Size: 375 1008 127 2330
Fragments arranged by size:
2330 1499 1305 1119 1008 588 375 338
268 195 127 84
MspA1I CmG'CkG
Cuts at: 279 355 1672 1877 3031 3694 4106 4522 4828
Size: 76 1317 205 1154 663 412 416 306
Cuts at: 4828 6347 6661 7021 8034 8279 279
Size: 1519 314 360 1013 245 1236
Fragments arranged by size:
1519 1317 1236 1154 1013 663 416 412
360 314 306 245 205 76
NaeI GCC'GGC
Cuts at: 7417 7417
Size: 9236
NarI GG'CG_CC
Cuts at: 6667 6915 6667
Size: 248 8988
NdeI CA'TA_TG
Cuts at: 2798 4154 2798
Size: 1356 7880
NgoMIV G'CCGG_C
Cuts at: 7415 7415
Size: 9236
NsiI A_TGCA'T
Cuts at: 5577 5829 5577
Size: 252 8984
NspI r_CATG'y
Cuts at: 145 3676 5105 6289 6789 7696 145
Size: 3531 1429 1184 500 907 1685
Fragments arranged by size:
3531 1685 1429 1184 907 500
PasI CC'CwG_GG
Cuts at: 498 498
Size: 9236
PciI A'CATG_T
Cuts at: 3672 7692 3672
Size: 4020 5216
PflMI CCAn_nnn'nTGG
Cuts at: 403 1950 4885 5527 403
Size: 1547 2935 642 4112
Fragments arranged by size:
4112 2935 1547 642
PfoI T'CCnGG_A
Cuts at: 4219 4434 4219
Size: 215 9021
PmeI GTTT'AAAC
Cuts at: 6235 6235
Size: 9236
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8375 8407 8375
Size: 32 9204
PpuMI rG'GwC_Cy
Cuts at: 427 1861 5962 427
Size: 1434 4101 3701
Fragments arranged by size:
4101 3701 1434
PshAI GACnn'nnGTC
Cuts at: 6107 6107
Size: 9236
PsiI TTA'TAA
Cuts at: 2293 3835 2293
Size: 1542 7694
PspOMI G'GGCC_C
Cuts at: 134 5622 134
Size: 5488 3748
PstI C_TGCA'G
Cuts at: 230 737 825 3381 4266 5294 6283 6968 230
Size: 507 88 2556 885 1028 989 685 2498
Fragments arranged by size:
2556 2498 1028 989 885 685 507 88
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9236
PvuII CAG'CTG
Cuts at: 279 355 1672 3031 3694 4106 4522 4828 6661
Size: 76 1317 1359 663 412 416 306 1833
Cuts at: 6661 7021 279
Size: 360 2494
Fragments arranged by size:
2494 1833 1359 1317 663 416 412 360
306 76
RsrII CG'GwC_CG
Cuts at: 7431 7431
Size: 9236
SacI G_AGCT'C
Cuts at: 527 1517 3067 6250 527
Size: 990 1550 3183 3513
Fragments arranged by size:
3513 3183 1550 990
SalI G'TCGA_C
Cuts at: 6273 6273
Size: 9236
SapI GCTCTTCn'nnn_
Cuts at: 1891 7259 7469 1891
Size: 5368 210 3658
Fragments arranged by size:
5368 3658 210
SbfI CC_TGCA'GG
Cuts at: 6283 6283
Size: 9236
ScaI AGT'ACT
Cuts at: 2786 8753 2786
Size: 5967 3269
SexAI A'CCwGG_T
Cuts at: 287 429 287
Size: 142 9094
SfcI C'TryA_G
Cuts at: 33 226 733 821 1976 2408 3377 3809 4262
Size: 193 507 88 1155 432 969 432 453
Cuts at: 4262 4700 5232 5290 6279 6964 7957 8148 33
Size: 438 532 58 989 685 993 191 1121
Fragments arranged by size:
1155 1121 993 989 969 685 532 507
453 438 432 432 193 191 88 58
SfoI GGC'GCC
Cuts at: 6668 6916 6668
Size: 248 8988
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9236
SmaI CCC'GGG
Cuts at: 6258 6258
Size: 9236
SmlI C'TyrA_G
Cuts at: 2398 2672 5160 6391 7306 7798 8060 2398
Size: 274 2488 1231 915 492 262 3574
Fragments arranged by size:
3574 2488 1231 915 492 274 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9236
SphI G_CATG'C
Cuts at: 145 6289 6789 145
Size: 6144 500 2592
Fragments arranged by size:
6144 2592 500
SspI AAT'ATT
Cuts at: 297 321 602 297
Size: 24 281 8931
Fragments arranged by size:
8931 281 24
StuI AGG'CCT
Cuts at: 548 5312 548
Size: 4764 4472
StyI C'CwwG_G
Cuts at: 549 1260 3159 3844 4568 5313 6370 6724 549
Size: 711 1899 685 724 745 1057 354 3061
Fragments arranged by size:
3061 1899 1057 745 724 711 685 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1152 2031 2038 2369 3966 4229 5050 5473 5723
Size: 879 7 331 1597 263 821 423 250
Cuts at: 5723 5815 6181 6819 6952 7411 7620 1152
Size: 92 366 638 133 459 209 2768
Fragments arranged by size:
2768 1597 879 821 638 459 423 366
331 263 250 209 133 92 7
TatI w'GTAC_w
Cuts at: 270 2155 2784 3890 5536 8751 270
Size: 1885 629 1106 1646 3215 755
Fragments arranged by size:
3215 1885 1646 1106 755 629
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1454 1486 5251 5283 5342 5374 5852 5884 8753
Size: 32 3765 32 59 32 478 32 2869
Cuts at: 8753 8785 1454
Size: 32 1905
Fragments arranged by size:
3765 2869 1905 478 59 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 5368 7033 5368
Size: 1665 7571
XbaI T'CTAG_A
Cuts at: 4302 6153 6267 4302
Size: 1851 114 7271
Fragments arranged by size:
7271 1851 114
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6731 6731
Size: 9236
XmaI C'CCGG_G
Cuts at: 6256 6256
Size: 9236
XmnI GAAnn'nnTTC
Cuts at: 1885 2165 3336 3788 4475 5444 1885
Size: 280 1171 452 687 969 5677
Fragments arranged by size:
5677 1171 969 687 452 280
ZraI GAC'GTC
Cuts at: 1533 5933 6109 1533
Size: 4400 176 4660
Fragments arranged by size:
4660 4400 176
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AscI BcgI BmtI BsiWI BssHII BstZ17I ClaI
CspCI EcoNI EcoRV FseI FspAI NcoI NheI NotI
NruI PacI PmlI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SwaI XhoI