Restriction map for
pF1KB0067
(Circular) (Six-base) MAPSORT of: pF1KB0067.seq Check: 2180 from: 1 to: 10439
pF1KB0067 10439 bp
With 182 enzymes: SgfI *
May 11, 2009 09:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5024 5233 6351 5024
Size: 209 1118 9112
Fragments arranged by size:
9112 1118 209
AatII G_ACGT'C
Cuts at: 3578 3578
Size: 10439
AccI GT'mk_AC
Cuts at: 5057 5219 7477 5057
Size: 162 2258 8019
Fragments arranged by size:
8019 2258 162
Acc65I G'GTAC_C
Cuts at: 7455 7455
Size: 10439
AclI AA'CG_TT
Cuts at: 1277 10279 1277
Size: 9002 1437
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 476 1043 3287 4001 4184 4709 6349 8264 8696
Size: 567 2244 714 183 525 1640 1915 432
Cuts at: 8696 9049 9711 476
Size: 353 662 1204
Fragments arranged by size:
2244 1915 1640 1204 714 662 567 525
432 353 183
AfeI AGC'GCT
Cuts at: 5428 6303 5428
Size: 875 9564
AflIII A'CryG_T
Cuts at: 1890 2024 4943 5450 7791 9591 9872 1890
Size: 134 2919 507 2341 1800 281 2457
Fragments arranged by size:
2919 2457 2341 1800 507 281 134
AgeI A'CCGG_T
Cuts at: 1864 1864
Size: 10439
AhdI GACnn_n'nnGTC
Cuts at: 1298 3426 7270 1298
Size: 2128 3844 4467
Fragments arranged by size:
4467 3844 2128
AleI CACnn'nnGTG
Cuts at: 982 1069 5902 982
Size: 87 4833 5519
Fragments arranged by size:
5519 4833 87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 406 1520 5840 6776 406
Size: 1114 4320 936 4069
Fragments arranged by size:
4320 4069 1114 936
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1786 1818 1786
Size: 32 10407
AlwNI CAG_nnn'CTG
Cuts at: 295 402 1150 1179 1905 2487 3076 4968 5442
Size: 107 748 29 726 582 589 1892 474
Cuts at: 5442 5751 6021 6366 6531 6699 7150 7744 9182
Size: 309 270 345 165 168 451 594 1438
Cuts at: 9182 295
Size: 1552
Fragments arranged by size:
1892 1552 1438 748 726 594 589 582
474 451 345 309 270 168 165 107
29
ApaI G_GGCC'C
Cuts at: 536 6140 6260 536
Size: 5604 120 4715
Fragments arranged by size:
5604 4715 120
ApaLI G'TGCA_C
Cuts at: 2438 2962 5446 9277 2438
Size: 524 2484 3831 3600
Fragments arranged by size:
3831 3600 2484 524
ApoI r'AATT_y
Cuts at: 1463 2271 3470 4075 4138 7443 9792 10048 1463
Size: 808 1199 605 63 3305 2349 256 1854
Fragments arranged by size:
3305 2349 1854 1199 808 605 256 63
AseI AT'TA_AT
Cuts at: 20 701 20
Size: 681 9758
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10439
AvaI C'yCGr_G
Cuts at: 810 2169 3623 4225 5776 6078 6160 7459 810
Size: 1359 1454 602 1551 302 82 1299 3790
Fragments arranged by size:
3790 1551 1454 1359 1299 602 302 82
BamHI G'GATC_C
Cuts at: 7464 7464
Size: 10439
BanI G'GyrC_C
Cuts at: 805 2466 2577 4148 5931 6177 7455 7869 8117
Size: 1661 111 1571 1783 246 1278 414 248
Cuts at: 8117 8152 805
Size: 35 3092
Fragments arranged by size:
3092 1783 1661 1571 1278 414 248 246
111 35
BanII G_rGCy'C
Cuts at: 472 536 2178 2380 3131 3158 3557 5398 6140
Size: 64 1642 202 751 27 399 1841 742
Cuts at: 6140 6260 7054 7453 8483 472
Size: 120 794 399 1030 2428
Fragments arranged by size:
2428 1841 1642 1030 794 751 742 399
399 202 120 64 27
BbeI G_GCGC'C
Cuts at: 809 2470 4152 6181 7873 8121 809
Size: 1661 1682 2029 1692 248 3127
Fragments arranged by size:
3127 2029 1692 1682 1661 248
BbsI GAAGACnn'nnnn_
Cuts at: 2780 3106 4130 4697 4992 2780
Size: 326 1024 567 295 8227
Fragments arranged by size:
8227 1024 567 326 295
BbvCI CC'TCA_GC
Cuts at: 1828 2417 5741 5766 6347 1828
Size: 589 3324 25 581 5920
Fragments arranged by size:
5920 3324 589 581 25
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 801 835 2943 2977 7306 7340 801
Size: 34 2108 34 4329 34 3900
Fragments arranged by size:
4329 3900 2108 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7199 8337 9393 10439 7199
Size: 1138 1056 1046 7199
Fragments arranged by size:
7199 1138 1056 1046
BclI T'GATC_A
Cuts at: 4575 7959 4575
Size: 3384 7055
BfrBI ATG'CAT
Cuts at: 3978 3978
Size: 10439
BglI GCCn_nnn'nGGC
Cuts at: 407 2173 4599 6162 6244 6544 6769 407
Size: 1766 2426 1563 82 300 225 4077
Fragments arranged by size:
4077 2426 1766 1563 300 225 82
BglII A'GATC_T
Cuts at: 7954 9991 7954
Size: 2037 8402
BlpI GC'TnA_GC
Cuts at: 2177 5074 5416 7551 2177
Size: 2897 342 2135 5065
Fragments arranged by size:
5065 2897 2135 342
Bme1580I G_kGCm'C
Cuts at: 536 2442 2828 2966 4517 5450 5630 6116 6140
Size: 1906 386 138 1551 933 180 486 24
Cuts at: 6140 6260 6791 8064 8157 9281 536
Size: 120 531 1273 93 1124 1694
Fragments arranged by size:
1906 1694 1551 1273 1124 933 531 486
386 180 138 120 93 24
BmgBI CAC'GTC
Cuts at: 611 4347 7118 7233 611
Size: 3736 2771 115 3817
Fragments arranged by size:
3817 3736 2771 115
BmrI ACTGGGnnnn_n'
Cuts at: 926 987 2822 2899 4117 5142 7164 7651 7732
Size: 61 1835 77 1218 1025 2022 487 81
Cuts at: 7732 8067 10211 926
Size: 335 2144 1154
Fragments arranged by size:
2144 2022 1835 1218 1154 1025 487 335
81 77 61
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 172 5160 8806 172
Size: 4988 3646 1805
Fragments arranged by size:
4988 3646 1805
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 776 977 1001 1436 2630 2698 6454 6859 7127
Size: 201 24 435 1194 68 3756 405 268
Cuts at: 7127 776
Size: 4088
Fragments arranged by size:
4088 3756 1194 435 405 268 201 68
24
Bpu10I CC'TnA_GC
Cuts at: 1828 2417 5741 5766 6347 1828
Size: 589 3324 25 581 5920
Fragments arranged by size:
5920 3324 589 581 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 2246 6794 7615 8494 9208 9506 401
Size: 1845 4548 821 879 714 298 1334
Fragments arranged by size:
4548 1845 1334 879 821 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 823 1454 2205 3100 7145 10143 823
Size: 631 751 895 4045 2998 1119
Fragments arranged by size:
4045 2998 1119 895 751 631
BsaAI yAC'GTr
Cuts at: 3567 5247 6432 7792 3567
Size: 1680 1185 1360 6214
Fragments arranged by size:
6214 1680 1360 1185
BsaBI GATnn'nnATC
Cuts at: 6 7953 9990 6
Size: 7947 2037 455
Fragments arranged by size:
7947 2037 455
BsaHI Gr'CG_yC
Cuts at: 806 2467 2956 3575 4149 5102 5812 6178 7870
Size: 1661 489 619 574 953 710 366 1692
Cuts at: 7870 8118 8820 10316 806
Size: 248 702 1496 929
Fragments arranged by size:
1692 1661 1496 953 929 710 702 619
574 489 366 248
BsaWI w'CCGG_w
Cuts at: 1864 7628 8149 8827 9238 9385 9659 1864
Size: 5764 521 678 411 147 274 2644
Fragments arranged by size:
5764 2644 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1528 1558 1786 1816 1833 1863 2106 2136 2664
Size: 30 228 30 17 30 243 30 528
Cuts at: 2664 2694 2796 2826 3145 3175 4424 4454 5639
Size: 30 102 30 319 30 1249 30 1185
Cuts at: 5639 5669 1528
Size: 30 6298
Fragments arranged by size:
6298 1249 1185 528 319 243 228 102
30 30 30 30 30 30 30 30
30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 266 347 661 1841 2732 3024 3190 3385 3454
Size: 81 314 1180 891 292 166 195 69
Cuts at: 3454 4804 5133 266
Size: 1350 329 5572
Fragments arranged by size:
5572 1350 1180 891 329 314 292 195
166 81 69
BseYI C'CCAG_C
Cuts at: 499 1306 1540 2613 2747 2969 3062 3458 4930
Size: 807 234 1073 134 222 93 396 1472
Cuts at: 4930 5023 5346 5693 5797 5828 5872 5954 6005
Size: 93 323 347 104 31 44 82 51
Cuts at: 6005 6148 6188 6253 6268 6298 6337 6458 6476
Size: 143 40 65 15 30 39 121 18
Cuts at: 6476 6494 6517 6772 6942 7055 7175 7214 7337
Size: 18 23 255 170 113 120 39 123
Cuts at: 7337 7351 7864 8622 9287 9695 499
Size: 14 513 758 665 408 1243
Fragments arranged by size:
1472 1243 1073 807 758 665 513 408
396 347 323 255 234 222 170 143
134 123 121 120 113 104 93 93
82 65 51 44 40 39 39 31
30 23 18 18 15 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 1356 2354 2650 3979 5002 5176 5250 5533
Size: 944 998 296 1329 1023 174 74 283
Cuts at: 5533 6295 412
Size: 762 4556
Fragments arranged by size:
4556 1329 1023 998 944 762 296 283
174 74
BsiEI CG_ry'CG
Cuts at: 104 6248 7981 8027 9257 104
Size: 6144 1733 46 1230 1286
Fragments arranged by size:
6144 1733 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2442 2851 2966 4813 5450 7453 8231 8421 9281
Size: 409 115 1847 637 2003 778 190 860
Cuts at: 9281 2442
Size: 3600
Fragments arranged by size:
3600 2003 1847 860 778 637 409 190
115
BsmI GAATG_Cn'
Cuts at: 645 782 1509 2417 4346 4863 6540 645
Size: 137 727 908 1929 517 1677 4544
Fragments arranged by size:
4544 1929 1677 908 727 517 137
BsmBI CGTCTCn'nnnn_
Cuts at: 1206 5245 7239 1206
Size: 4039 1994 4406
Fragments arranged by size:
4406 4039 1994
Bsp1286I G_dGCh'C
Cuts at: 472 536 2178 2380 2442 2828 2851 2966 3131
Size: 64 1642 202 62 386 23 115 165
Cuts at: 3131 3158 3557 4517 4813 5398 5450 5630 6116
Size: 27 399 960 296 585 52 180 486
Cuts at: 6116 6140 6260 6791 7054 7453 8064 8157 8231
Size: 24 120 531 263 399 611 93 74
Cuts at: 8231 8421 8483 9281 472
Size: 190 62 798 1630
Fragments arranged by size:
1642 1630 960 798 611 585 531 486
399 399 386 296 263 202 190 180
165 120 115 93 74 64 62 62
52 27 24 23
BspHI T'CATG_A
Cuts at: 731 6626 8871 731
Size: 5895 2245 2299
Fragments arranged by size:
5895 2299 2245
BspMI ACCTGCnnnn'nnnn_
Cuts at: 831 1913 5024 5233 6323 6351 7489 7703 8005
Size: 1082 3111 209 1090 28 1138 214 302
Cuts at: 8005 8386 831
Size: 381 2884
Fragments arranged by size:
3111 2884 1138 1090 1082 381 302 214
209 28
BsrBI CCG'CTC
Cuts at: 50 2473 4647 5156 6134 8731 8785 10269 10434
Size: 2423 2174 509 978 2597 54 1484 165
Cuts at: 10434 50
Size: 55
Fragments arranged by size:
2597 2423 2174 1484 978 509 165 55
54
BsrDI GCAATG_nn'
Cuts at: 4560 7334 8351 4560
Size: 2774 1017 6648
Fragments arranged by size:
6648 2774 1017
BsrFI r'CCGG_y
Cuts at: 1864 5411 5705 5934 6763 6831 8437 8618 1864
Size: 3547 294 229 829 68 1606 181 3685
Fragments arranged by size:
3685 3547 1606 829 294 229 181 68
BsrGI T'GTAC_A
Cuts at: 4394 4394
Size: 10439
BssSI C'ACGA_G
Cuts at: 2330 4383 8710 9418 2330
Size: 2053 4327 708 3351
Fragments arranged by size:
4327 3351 2053 708
BstAPI GCAn_nnn'nTGC
Cuts at: 5234 5273 5300 5682 5751 6201 6315 6699 6855
Size: 39 27 382 69 450 114 384 156
Cuts at: 6855 6978 7065 7816 5234
Size: 123 87 751 7857
Fragments arranged by size:
7857 751 450 384 382 156 123 114
87 69 39 27
BstBI TT'CG_AA
Cuts at: 8800 8800
Size: 10439
BstEII G'GTnAC_C
Cuts at: 2883 2883
Size: 10439
BstXI CCAn_nnnn'nTGG
Cuts at: 2292 2977 4107 6432 2292
Size: 685 1130 2325 6299
Fragments arranged by size:
6299 2325 1130 685
BstYI r'GATC_y
Cuts at: 1 739 1146 3453 3836 4463 5669 7464 7931
Size: 738 407 2307 383 627 1206 1795 467
Cuts at: 7931 7954 8289 8535 8841 8853 8939 9991 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
2307 1795 1206 1052 738 627 467 449
407 383 335 306 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1613 2621 1613
Size: 1008 9431
BtgI C'CryG_G
Cuts at: 107 458 2321 2690 3503 4979 5900 6248 6556
Size: 351 1863 369 813 1476 921 348 308
Cuts at: 6556 6887 7162 7201 7277 7397 107
Size: 331 275 39 76 120 3149
Fragments arranged by size:
3149 1863 1476 921 813 369 351 348
331 308 275 120 76 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2536 2924 3791 5559 6601 8389 8570 8733 2536
Size: 388 867 1768 1042 1788 181 163 4242
Fragments arranged by size:
4242 1788 1768 1042 867 388 181 163
BtsI GCAGTG_nn'
Cuts at: 1139 2662 4811 5262 5601 6172 7682 1139
Size: 1523 2149 451 339 571 1510 3896
Fragments arranged by size:
3896 2149 1523 1510 571 451 339
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3230 3265 3719 3754 3945 3980 3230
Size: 35 454 35 191 35 9689
Fragments arranged by size:
9689 454 191 35 35 35
DraI TTT'AAA
Cuts at: 269 3363 7438 269
Size: 3094 4075 3270
Fragments arranged by size:
4075 3270 3094
DraIII CAC_nnn'GTG
Cuts at: 5247 5273 5247
Size: 26 10413
DrdI GACnn_nn'nnGTC
Cuts at: 1385 5215 6330 8145 9489 1385
Size: 3830 1115 1815 1344 2335
Fragments arranged by size:
3830 2335 1815 1344 1115
EaeI y'GGCC_r
Cuts at: 408 443 687 899 1623 2393 3041 3966 4193
Size: 35 244 212 724 770 648 925 227
Cuts at: 4193 4429 5714 6245 6434 6792 6833 6921 7101
Size: 236 1285 531 189 358 41 88 180
Cuts at: 7101 7280 8024 8198 8589 8616 9961 408
Size: 179 744 174 391 27 1345 886
Fragments arranged by size:
1345 1285 925 886 770 744 724 648
531 391 358 244 236 227 212 189
180 179 174 88 41 35 27
EagI C'GGCC_G
Cuts at: 6245 8024 6245
Size: 1779 8660
EarI CTCTTCn'nnn_
Cuts at: 1763 2597 3290 4751 4781 4799 5308 8462 8672
Size: 834 693 1461 30 18 509 3154 210
Cuts at: 8672 1763
Size: 3530
Fragments arranged by size:
3530 3154 1461 834 693 509 210 30
18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5647 5724 8647 9389 9535 10249 5647
Size: 77 2923 742 146 714 5837
Fragments arranged by size:
5837 2923 742 714 146 77
EcoICRI GAG'CTC
Cuts at: 7451 7451
Size: 10439
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 476 776 977 1001 1043 1436 2630 2698 3287
Size: 300 201 24 42 393 1194 68 589
Cuts at: 3287 4001 4184 4709 6349 6454 6859 7127 8264
Size: 714 183 525 1640 105 405 268 1137
Cuts at: 8264 8696 9049 9711 476
Size: 432 353 662 1204
Fragments arranged by size:
1640 1204 1194 1137 714 662 589 525
432 405 393 353 300 268 201 183
105 68 42 24
EcoNI CCTnn'n_nnAGG
Cuts at: 838 5686 838
Size: 4848 5591
EcoO109I rG'GnC_Cy
Cuts at: 399 1135 1902 2326 2617 3073 3384 3420 5780
Size: 736 767 424 291 456 311 36 2360
Cuts at: 5780 6074 6257 6814 7025 7578 399
Size: 294 183 557 211 553 3260
Fragments arranged by size:
3260 2360 767 736 557 553 456 424
311 294 291 211 183 36
EcoRI G'AATT_C
Cuts at: 1463 2271 3470 7443 1463
Size: 808 1199 3973 4459
Fragments arranged by size:
4459 3973 1199 808
EcoRV GAT'ATC
Cuts at: 1047 6227 1047
Size: 5180 5259
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5016 8458 5016
Size: 3442 6997
FspI TGC'GCA
Cuts at: 8220 8220
Size: 10439
HaeII r_GCGC'y
Cuts at: 380 809 2470 4152 5430 6181 6305 7873 8121
Size: 429 1661 1682 1278 751 124 1568 248
Cuts at: 8121 9351 10122 380
Size: 1230 771 697
Fragments arranged by size:
1682 1661 1568 1278 1230 771 751 697
429 248 124
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 184 216 614 646 1284 1316 1337 1369 1528
Size: 32 398 32 638 32 21 32 159
Cuts at: 1528 1560 1650 1682 2727 2759 3145 3177 3217
Size: 32 90 32 1045 32 386 32 40
Cuts at: 3217 3249 3412 3444 5371 5403 6648 6680 6888
Size: 32 163 32 1927 32 1245 32 208
Cuts at: 6888 6920 7256 7288 8281 8311 8313 8343 184
Size: 32 336 32 993 30 2 30 2280
Fragments arranged by size:
2280 1927 1245 1045 993 638 398 386
336 208 163 159 90 40 32 32
32 32 32 32 32 32 32 32
32 32 32 32 30 30 21 2
HincII GTy'rAC
Cuts at: 1020 3329 5058 7478 1020
Size: 2309 1729 2420 3981
Fragments arranged by size:
3981 2420 2309 1729
HindIII A'AGCT_T
Cuts at: 3646 3646
Size: 10439
KasI G'GCGC_C
Cuts at: 805 2466 4148 6177 7869 8117 805
Size: 1661 1682 2029 1692 248 3127
Fragments arranged by size:
3127 2029 1692 1682 1661 248
KpnI G_GTAC'C
Cuts at: 7459 7459
Size: 10439
MfeI C'AATT_G
Cuts at: 1124 1475 1124
Size: 351 10088
MluI A'CGCG_T
Cuts at: 5450 9872 5450
Size: 4422 6017
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 299 1786 1829 9198 9382 299
Size: 1487 43 7369 184 1356
Fragments arranged by size:
7369 1487 1356 184 43
MscI TGG'CCA
Cuts at: 410 445 689 901 1625 2395 3043 4195 5716
Size: 35 244 212 724 770 648 1152 1521
Cuts at: 5716 6436 6923 7103 7282 8200 410
Size: 720 487 180 179 918 2649
Fragments arranged by size:
2649 1521 1152 918 770 724 720 648
487 244 212 180 179 35
MslI CAynn'nnrTG
Cuts at: 982 1069 1889 3815 5182 5902 6067 8428 8555
Size: 87 820 1926 1367 720 165 2361 127
Cuts at: 8555 982
Size: 2866
Fragments arranged by size:
2866 2361 1926 1367 820 720 165 127
87
MspA1I CmG'CkG
Cuts at: 341 544 1179 1605 2516 3759 5078 5863 5971
Size: 203 635 426 911 1243 1319 785 108
Cuts at: 5971 6558 6696 6987 7341 7550 7864 8224 9008
Size: 587 138 291 354 209 314 360 784
Cuts at: 9008 9253 341
Size: 245 1527
Fragments arranged by size:
1527 1319 1243 911 785 784 635 587
426 360 354 314 291 245 209 203
138 108
NaeI GCC'GGC
Cuts at: 5413 5707 5936 6765 8620 5413
Size: 294 229 829 1855 7232
Fragments arranged by size:
7232 1855 829 294 229
NarI GG'CG_CC
Cuts at: 806 2467 4149 6178 7870 8118 806
Size: 1661 1682 2029 1692 248 3127
Fragments arranged by size:
3127 2029 1692 1682 1661 248
NcoI C'CATG_G
Cuts at: 107 458 2321 3503 4979 6887 7277 107
Size: 351 1863 1182 1476 1908 390 3269
Fragments arranged by size:
3269 1908 1863 1476 1182 390 351
NgoMIV G'CCGG_C
Cuts at: 5411 5705 5934 6763 8618 5411
Size: 294 229 829 1855 7232
Fragments arranged by size:
7232 1855 829 294 229
NruI TCG'CGA
Cuts at: 3776 3776
Size: 10439
NsiI A_TGCA'T
Cuts at: 3980 3980
Size: 10439
NspI r_CATG'y
Cuts at: 1894 2028 2253 2613 2706 4938 5181 5856 6051
Size: 134 225 360 93 2232 243 675 195
Cuts at: 6051 6156 6453 6489 6507 6858 7068 7492 7992
Size: 105 297 36 18 351 210 424 500
Cuts at: 7992 9595 1894
Size: 1603 2738
Fragments arranged by size:
2738 2232 1603 675 500 424 360 351
297 243 225 210 195 134 105 93
36 18
PacI TTA_AT'TAA
Cuts at: 701 701
Size: 10439
PasI CC'CwG_GG
Cuts at: 1677 1770 3858 5199 6606 1677
Size: 93 2088 1341 1407 5510
Fragments arranged by size:
5510 2088 1407 1341 93
PciI A'CATG_T
Cuts at: 1890 2024 9591 1890
Size: 134 7567 2738
Fragments arranged by size:
7567 2738 134
PflMI CCAn_nnn'nTGG
Cuts at: 1459 5331 5442 1459
Size: 3872 111 6456
Fragments arranged by size:
6456 3872 111
PmeI GTTT'AAAC
Cuts at: 7438 7438
Size: 10439
PmlI CAC'GTG
Cuts at: 5247 5247
Size: 10439
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1786 1818 4275 4307 8885 8917 1786
Size: 32 2457 32 4578 32 3308
Fragments arranged by size:
4578 3308 2457 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1902 2326 3073 3420 5780 6074 6814 7025 1902
Size: 424 747 347 2360 294 740 211 5316
Fragments arranged by size:
5316 2360 747 740 424 347 294 211
PshAI GACnn'nnGTC
Cuts at: 2229 2229
Size: 10439
PspOMI G'GGCC_C
Cuts at: 532 6136 6256 532
Size: 5604 120 4715
Fragments arranged by size:
5604 4715 120
PspXI vC'TCGA_Gb
Cuts at: 810 810
Size: 10439
PstI C_TGCA'G
Cuts at: 1146 1610 4097 4607 4818 5687 5970 6527 6704
Size: 464 2487 510 211 869 283 557 177
Cuts at: 6704 6983 6998 7486 8171 1146
Size: 279 15 488 685 3414
Fragments arranged by size:
3414 2487 869 685 557 510 488 464
283 279 211 177 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10439
PvuII CAG'CTG
Cuts at: 1179 1605 3759 5078 5863 6696 7341 7864 8224
Size: 426 2154 1319 785 833 645 523 360
Cuts at: 8224 1179
Size: 3394
Fragments arranged by size:
3394 2154 1319 833 785 645 523 426
360
RsrII CG'GwC_CG
Cuts at: 130 8634 130
Size: 8504 1935
SacI G_AGCT'C
Cuts at: 7453 7453
Size: 10439
SacII CC_GC'GG
Cuts at: 6559 6559
Size: 10439
SalI G'TCGA_C
Cuts at: 5056 7476 5056
Size: 2420 8019
SanDI GG'GwC_CC
Cuts at: 5780 6074 7025 5780
Size: 294 951 9194
Fragments arranged by size:
9194 951 294
SapI GCTCTTCn'nnn_
Cuts at: 1763 2597 5308 8462 8672 1763
Size: 834 2711 3154 210 3530
Fragments arranged by size:
3530 3154 2711 834 210
SbfI CC_TGCA'GG
Cuts at: 7486 7486
Size: 10439
ScaI AGT'ACT
Cuts at: 9956 9956
Size: 10439
SexAI A'CCwGG_T
Cuts at: 624 1991 6125 7130 624
Size: 1367 4134 1005 3933
Fragments arranged by size:
4134 3933 1367 1005
SfcI C'TryA_G
Cuts at: 33 1040 1142 1606 2855 3449 3789 4093 4603
Size: 1007 102 464 1249 594 340 304 510
Cuts at: 4603 4814 5683 5966 6523 6700 6979 6994 7482
Size: 211 869 283 557 177 279 15 488
Cuts at: 7482 8167 9135 9326 33
Size: 685 968 191 1146
Fragments arranged by size:
1249 1146 1007 968 869 685 594 557
510 488 464 340 304 283 279 211
191 177 102 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 407 6244 407
Size: 5837 4602
SfoI GGC'GCC
Cuts at: 807 2468 4150 6179 7871 8119 807
Size: 1661 1682 2029 1692 248 3127
Fragments arranged by size:
3127 2029 1692 1682 1661 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10439
SgrAI Cr'CCGG_yG
Cuts at: 1864 5705 1864
Size: 3841 6598
SmaI CCC'GGG
Cuts at: 2171 4227 5778 6080 6162 7461 2171
Size: 2056 1551 302 82 1299 5149
Fragments arranged by size:
5149 2056 1551 1299 302 82
SmlI C'TyrA_G
Cuts at: 416 810 2261 6809 7594 8509 9223 9485 416
Size: 394 1451 4548 785 915 714 262 1370
Fragments arranged by size:
4548 1451 1370 915 785 714 394 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 10439
SphI G_CATG'C
Cuts at: 2253 2706 4938 5181 6051 6156 6453 6489 6507
Size: 453 2232 243 870 105 297 36 18
Cuts at: 6507 6858 7492 7992 2253
Size: 351 634 500 4700
Fragments arranged by size:
4700 2232 870 634 500 453 351 297
243 105 36 18
SspI AAT'ATT
Cuts at: 2056 2056
Size: 10439
StuI AGG'CCT
Cuts at: 263 668 6219 6596 6616 7087 263
Size: 405 5551 377 20 471 3615
Fragments arranged by size:
5551 3615 471 405 377 20
StyI C'CwwG_G
Cuts at: 107 458 2291 2321 3503 3693 4709 4979 5522
Size: 351 1833 30 1182 190 1016 270 543
Cuts at: 5522 6887 7277 7573 7927 107
Size: 1365 390 296 354 2619
Fragments arranged by size:
2619 1833 1365 1182 1016 543 390 354
351 296 270 190 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 394 677 2334 2425 2684 3850 4142 4583 5212
Size: 283 1657 91 259 1166 292 441 629
Cuts at: 5212 6297 6312 8022 8155 8614 8823 394
Size: 1085 15 1710 133 459 209 2010
Fragments arranged by size:
2010 1710 1657 1166 1085 629 459 441
292 283 259 209 133 91 15
TatI w'GTAC_w
Cuts at: 4394 9954 4394
Size: 5560 4879
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1131 1163 2796 2828 4424 4456 9956 9988 1131
Size: 32 1633 32 1596 32 5500 32 1582
Fragments arranged by size:
5500 1633 1596 1582 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2655 3574 3679 8236 2655
Size: 919 105 4557 4858
Fragments arranged by size:
4858 4557 919 105
XbaI T'CTAG_A
Cuts at: 7470 7470
Size: 10439
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2454 5020 6894 7934 2454
Size: 2566 1874 1040 4959
Fragments arranged by size:
4959 2566 1874 1040
XhoI C'TCGA_G
Cuts at: 810 810
Size: 10439
XmaI C'CCGG_G
Cuts at: 2169 4225 5776 6078 6160 7459 2169
Size: 2056 1551 302 82 1299 5149
Fragments arranged by size:
5149 2056 1551 1299 302 82
XmnI GAAnn'nnTTC
Cuts at: 2500 3826 2500
Size: 1326 9113
ZraI GAC'GTC
Cuts at: 3576 3576
Size: 10439
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AscI AvrII BaeI BmtI BsiWI BspEI BssHII
BstZ17I ClaI FseI FspAI HpaI NdeI NheI NotI
PfoI PsiI PsrI SnaBI SrfI SwaI