Restriction map for
pF1KB0087
(Circular) (Six-base) MAPSORT of: pF1KB0087.seq Check: 4880 from: 1 to: 6533
pF1KB0087 6533 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 650 3571 650
Size: 2921 3612
Acc65I G'GTAC_C
Cuts at: 3549 3549
Size: 6533
AclI AA'CG_TT
Cuts at: 1118 6373 1118
Size: 5255 1278
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 770 1508 2642 2869 4358 4790 5537 5805 770
Size: 738 1134 227 1489 432 747 268 1498
Fragments arranged by size:
1498 1489 1134 747 738 432 268 227
AfeI AGC'GCT
Cuts at: 1937 2373 3051 1937
Size: 436 678 5419
Fragments arranged by size:
5419 678 436
AflIII A'CryG_T
Cuts at: 2141 3885 4989 5966 2141
Size: 1744 1104 977 2708
Fragments arranged by size:
2708 1744 1104 977
AgeI A'CCGG_T
Cuts at: 4921 4921
Size: 6533
AhdI GACnn_n'nnGTC
Cuts at: 1183 1183
Size: 6533
AleI CACnn'nnGTG
Cuts at: 148 830 880 1381 148
Size: 682 50 501 5300
Fragments arranged by size:
5300 682 501 50
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 349 349
Size: 6533
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2016 2048 2016
Size: 32 6501
AlwNI CAG_nnn'CTG
Cuts at: 2235 2388 2529 3156 3838 5405 2235
Size: 153 141 627 682 1567 3363
Fragments arranged by size:
3363 1567 682 627 153 141
ApaI G_GGCC'C
Cuts at: 724 1653 724
Size: 929 5604
ApaLI G'TGCA_C
Cuts at: 2470 5303 2470
Size: 2833 3700
ApoI r'AATT_y
Cuts at: 709 2731 3537 5886 6142 709
Size: 2022 806 2349 256 1100
Fragments arranged by size:
2349 2022 1100 806 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6533
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6533
AvaI C'yCGr_G
Cuts at: 2945 3553 2945
Size: 608 5925
BamHI G'GATC_C
Cuts at: 3521 3558 3521
Size: 37 6496
BanI G'GyrC_C
Cuts at: 985 2002 2212 3549 3963 4211 4246 985
Size: 1017 210 1337 414 248 35 3272
Fragments arranged by size:
3272 1337 1017 414 248 210 35
BanII G_rGCy'C
Cuts at: 420 724 782 1653 1685 1955 2636 3547 4577
Size: 304 58 871 32 270 681 911 1030
Cuts at: 4577 420
Size: 2376
Fragments arranged by size:
2376 1030 911 871 681 304 270 58
32
BbeI G_GCGC'C
Cuts at: 3967 4215 3967
Size: 248 6285
BbsI GAAGACnn'nnnn_
Cuts at: 194 2100 2857 3355 194
Size: 1906 757 498 3372
Fragments arranged by size:
3372 1906 757 498
BciVI GTATCCnnnnn_n'
Cuts at: 2627 4431 5192 6533 2627
Size: 1804 761 1341 2627
Fragments arranged by size:
2627 1804 1341 761
BclI T'GATC_A
Cuts at: 1467 4053 1467
Size: 2586 3947
BglII A'GATC_T
Cuts at: 227 2276 4048 6085 227
Size: 2049 1772 2037 675
Fragments arranged by size:
2049 2037 1772 675
BlpI GC'TnA_GC
Cuts at: 3645 3645
Size: 6533
Bme1580I G_kGCm'C
Cuts at: 724 1653 2005 2353 2474 2605 4158 4251 5307
Size: 929 352 348 121 131 1553 93 1056
Cuts at: 5307 724
Size: 1950
Fragments arranged by size:
1950 1553 1056 929 352 348 131 121
93
BmrI ACTGGGnnnn_n'
Cuts at: 2292 3745 3826 4161 6305 2292
Size: 1453 81 335 2144 2520
Fragments arranged by size:
2520 2144 1453 335 81
BmtI G_CTAG'C
Cuts at: 2378 2378
Size: 6533
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4900 4900
Size: 6533
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 604 1228 2301 2391 2406 3067 3345 394
Size: 210 624 1073 90 15 661 278 3582
Fragments arranged by size:
3582 1073 661 624 278 210 90 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 897 982 1532 2110 2382 3709 4588 5080
Size: 350 85 550 578 272 1327 879 492
Cuts at: 5080 5378 547
Size: 298 1702
Fragments arranged by size:
1702 1327 879 578 550 492 350 298
272 85
BsaI GGTCTCn'nnnn_
Cuts at: 451 1021 1088 1548 2437 6237 451
Size: 570 67 460 889 3800 747
Fragments arranged by size:
3800 889 747 570 460 67
BsaAI yAC'GTr
Cuts at: 123 675 3886 123
Size: 552 3211 2770
Fragments arranged by size:
3211 2770 552
BsaBI GATnn'nnATC
Cuts at: 6 2165 4047 6084 6
Size: 2159 1882 2037 455
Fragments arranged by size:
2159 2037 1882 455
BsaHI Gr'CG_yC
Cuts at: 3964 4212 4914 6410 3964
Size: 248 702 1496 4087
Fragments arranged by size:
4087 1496 702 248
BsaWI w'CCGG_w
Cuts at: 166 1277 3722 4243 4921 5195 5342 5753 166
Size: 1111 2445 521 678 274 147 411 946
Fragments arranged by size:
2445 1111 946 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1418 1448 2447 2477 1418
Size: 30 999 30 5474
Fragments arranged by size:
5474 999 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 601 1444 2426 3082 3523 601
Size: 843 982 656 441 3611
Fragments arranged by size:
3611 982 843 656 441
BseYI C'CCAG_C
Cuts at: 924 1100 1851 2006 2368 2568 3958 4716 5293
Size: 176 751 155 362 200 1390 758 577
Cuts at: 5293 5789 924
Size: 496 1668
Fragments arranged by size:
1668 1390 758 751 577 496 362 200
176 155
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1822 2221 1822
Size: 399 6134
BsiEI CG_ry'CG
Cuts at: 104 185 1983 4075 4121 5329 104
Size: 81 1798 2092 46 1208 1308
Fragments arranged by size:
2092 1798 1308 1208 81 46
BsiHKAI G_wGCw'C
Cuts at: 1388 1685 2474 3194 3317 3547 4325 4515 5307
Size: 297 789 720 123 230 778 190 792
Cuts at: 5307 1388
Size: 2614
Fragments arranged by size:
2614 792 789 778 720 297 230 190
123
BsmBI CGTCTCn'nnnn_
Cuts at: 514 514
Size: 6533
Bsp1286I G_dGCh'C
Cuts at: 420 724 782 1388 1653 1685 1955 2005 2353
Size: 304 58 606 265 32 270 50 348
Cuts at: 2353 2474 2605 2636 3194 3317 3547 4158 4251
Size: 121 131 31 558 123 230 611 93
Cuts at: 4251 4325 4515 4577 5307 420
Size: 74 190 62 730 1646
Fragments arranged by size:
1646 730 611 606 558 348 304 270
265 230 190 131 123 121 93 74
62 58 50 32 31
BspEI T'CCGG_A
Cuts at: 1277 5753 1277
Size: 4476 2057
BspHI T'CATG_A
Cuts at: 2439 5709 2439
Size: 3270 3263
BspMI ACCTGCnnnn'nnnn_
Cuts at: 341 929 3583 3797 4099 4480 341
Size: 588 2654 214 302 381 2394
Fragments arranged by size:
2654 2394 588 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3072 3345 4825 4879 6363 6528 50
Size: 3022 273 1480 54 1484 165 55
Fragments arranged by size:
3022 1484 1480 273 165 55 54
BsrDI GCAATG_nn'
Cuts at: 432 1761 2615 4445 432
Size: 1329 854 1830 2520
Fragments arranged by size:
2520 1830 1329 854
BsrFI r'CCGG_y
Cuts at: 460 941 4531 4712 4921 460
Size: 481 3590 181 209 2072
Fragments arranged by size:
3590 2072 481 209 181
BssSI C'ACGA_G
Cuts at: 216 1297 2340 4804 5162 216
Size: 1081 1043 2464 358 1587
Fragments arranged by size:
2464 1587 1081 1043 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2813 3910 2813
Size: 1097 5436
BstBI TT'CG_AA
Cuts at: 4894 4894
Size: 6533
BstEII G'GTnAC_C
Cuts at: 237 3225 237
Size: 2988 3545
BstXI CCAn_nnnn'nTGG
Cuts at: 1563 3091 1563
Size: 1528 5005
BstYI r'GATC_y
Cuts at: 1 227 1002 1667 2225 2276 3328 3521 3558
Size: 226 775 665 558 51 1052 193 37
Cuts at: 3558 4025 4048 4383 4629 5641 5727 5739 6085
Size: 467 23 335 246 1012 86 12 346
Cuts at: 6085 1
Size: 449
Fragments arranged by size:
1052 1012 775 665 558 467 449 346
335 246 226 193 86 51 37 23
12
Bsu36I CC'TnA_GG
Cuts at: 662 1586 1676 662
Size: 924 90 5519
Fragments arranged by size:
5519 924 90
BtgI C'CryG_G
Cuts at: 420 758 1154 2918 3132 420
Size: 338 396 1764 214 3821
Fragments arranged by size:
3821 1764 396 338 214
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4483 4664 4827 4483
Size: 181 163 6189
Fragments arranged by size:
6189 181 163
BtsI GCAGTG_nn'
Cuts at: 1342 2403 2429 3479 3776 1342
Size: 1061 26 1050 297 4099
Fragments arranged by size:
4099 1061 1050 297 26
ClaI AT'CG_AT
Cuts at: 2219 2219
Size: 6533
DraI TTT'AAA
Cuts at: 3532 3532
Size: 6533
DraIII CAC_nnn'GTG
Cuts at: 2813 2813
Size: 6533
DrdI GACnn_nn'nnGTC
Cuts at: 3234 4239 5097 3234
Size: 1005 858 4670
Fragments arranged by size:
4670 1005 858
EaeI y'GGCC_r
Cuts at: 182 444 1151 1339 2186 4118 4292 4683 4710
Size: 262 707 188 847 1932 174 391 27
Cuts at: 4710 6055 182
Size: 1345 660
Fragments arranged by size:
1932 1345 847 707 660 391 262 188
174 27
EagI C'GGCC_G
Cuts at: 182 4118 182
Size: 3936 2597
EarI CTCTTCn'nnn_
Cuts at: 952 2453 2618 2930 3199 4556 4766 952
Size: 1501 165 312 269 1357 210 2719
Fragments arranged by size:
2719 1501 1357 312 269 210 165
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 568 4741 5051 5197 6343 568
Size: 4173 310 146 1146 758
Fragments arranged by size:
4173 1146 758 310 146
EcoICRI GAG'CTC
Cuts at: 1683 3545 1683
Size: 1862 4671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 604 770 1228 1508 2301 2391 2406 2642
Size: 210 166 458 280 793 90 15 236
Cuts at: 2642 2869 3067 3345 4358 4790 5537 5805 394
Size: 227 198 278 1013 432 747 268 1122
Fragments arranged by size:
1122 1013 793 747 458 432 280 278
268 236 227 210 198 166 90 15
EcoNI CCTnn'n_nnAGG
Cuts at: 526 3085 3496 526
Size: 2559 411 3563
Fragments arranged by size:
3563 2559 411
EcoO109I rG'GnC_Cy
Cuts at: 330 657 720 721 1177 1649 1650 2310 3672
Size: 327 63 1 456 472 1 660 1362
Cuts at: 3672 330
Size: 3191
Fragments arranged by size:
3191 1362 660 472 456 327 63 1
1
EcoRI G'AATT_C
Cuts at: 709 3537 709
Size: 2828 3705
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2880 4552 2880
Size: 1672 4861
FspI TGC'GCA
Cuts at: 2806 4314 2806
Size: 1508 5025
HaeII r_GCGC'y
Cuts at: 1939 2185 2375 3053 3967 4215 5237 6216 1939
Size: 246 190 678 914 248 1022 979 2256
Fragments arranged by size:
2256 1022 979 914 678 248 246 190
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 212 244 1169 1201 1659 1691 1854 1886 4375
Size: 32 925 32 458 32 163 32 2489
Cuts at: 4375 4405 4407 4437 212
Size: 30 2 30 2308
Fragments arranged by size:
2489 2308 925 458 163 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1122 2121 3207 3572 1122
Size: 999 1086 365 4083
Fragments arranged by size:
4083 1086 999 365
HindIII A'AGCT_T
Cuts at: 752 752
Size: 6533
KasI G'GCGC_C
Cuts at: 3963 4211 3963
Size: 248 6285
KpnI G_GTAC'C
Cuts at: 3553 3553
Size: 6533
MluI A'CGCG_T
Cuts at: 5966 5966
Size: 6533
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5204 5388 5204
Size: 184 6349
MscI TGG'CCA
Cuts at: 1153 1341 2188 4294 1153
Size: 188 847 2106 3392
Fragments arranged by size:
3392 2106 847 188
MslI CAynn'nnrTG
Cuts at: 148 425 830 880 1301 1381 1603 1705 1882
Size: 277 405 50 421 80 222 102 177
Cuts at: 1882 2608 2872 4522 4649 148
Size: 726 264 1650 127 2032
Fragments arranged by size:
2032 1650 726 421 405 277 264 222
177 127 102 80 50
MspA1I CmG'CkG
Cuts at: 1230 3461 3644 3958 4318 5331 5576 1230
Size: 2231 183 314 360 1013 245 2187
Fragments arranged by size:
2231 2187 1013 360 314 245 183
NaeI GCC'GGC
Cuts at: 943 4714 943
Size: 3771 2762
NarI GG'CG_CC
Cuts at: 3964 4212 3964
Size: 248 6285
NcoI C'CATG_G
Cuts at: 420 758 1154 2918 420
Size: 338 396 1764 4035
Fragments arranged by size:
4035 1764 396 338
NgoMIV G'CCGG_C
Cuts at: 941 4712 941
Size: 3771 2762
NheI G'CTAG_C
Cuts at: 2374 2374
Size: 6533
NruI TCG'CGA
Cuts at: 548 548
Size: 6533
NspI r_CATG'y
Cuts at: 279 598 1306 2145 3270 3586 4086 4993 279
Size: 319 708 839 1125 316 500 907 1819
Fragments arranged by size:
1819 1125 907 839 708 500 319 316
PciI A'CATG_T
Cuts at: 2141 4989 2141
Size: 2848 3685
PflMI CCAn_nnn'nTGG
Cuts at: 1632 2103 1632
Size: 471 6062
PfoI T'CCnGG_A
Cuts at: 371 431 3083 3398 371
Size: 60 2652 315 3506
Fragments arranged by size:
3506 2652 315 60
PmeI GTTT'AAAC
Cuts at: 3532 3532
Size: 6533
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5672 5704 5672
Size: 32 6501
PpuMI rG'GwC_Cy
Cuts at: 330 657 1177 2310 330
Size: 327 520 1133 4553
Fragments arranged by size:
4553 1133 520 327
PshAI GACnn'nnGTC
Cuts at: 810 810
Size: 6533
PspOMI G'GGCC_C
Cuts at: 720 1649 720
Size: 929 5604
PstI C_TGCA'G
Cuts at: 1667 2534 3486 3580 4265 1667
Size: 867 952 94 685 3935
Fragments arranged by size:
3935 952 867 685 94
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6533
PvuII CAG'CTG
Cuts at: 1230 3958 4318 1230
Size: 2728 360 3445
Fragments arranged by size:
3445 2728 360
RsrII CG'GwC_CG
Cuts at: 4728 4728
Size: 6533
SacI G_AGCT'C
Cuts at: 1685 3547 1685
Size: 1862 4671
SalI G'TCGA_C
Cuts at: 3570 3570
Size: 6533
SapI GCTCTTCn'nnn_
Cuts at: 952 3199 4556 4766 952
Size: 2247 1357 210 2719
Fragments arranged by size:
2719 2247 1357 210
SbfI CC_TGCA'GG
Cuts at: 3580 3580
Size: 6533
ScaI AGT'ACT
Cuts at: 6050 6050
Size: 6533
SfcI C'TryA_G
Cuts at: 33 1640 1663 2085 2530 2680 3442 3482 3576
Size: 1607 23 422 445 150 762 40 94
Cuts at: 3576 4261 5254 5445 33
Size: 685 993 191 1121
Fragments arranged by size:
1607 1121 993 762 685 445 422 191
150 94 40 23
SfoI GGC'GCC
Cuts at: 3965 4213 3965
Size: 248 6285
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6533
SmaI CCC'GGG
Cuts at: 3555 3555
Size: 6533
SmlI C'TyrA_G
Cuts at: 562 912 997 1547 2125 2361 2945 3688 4603
Size: 350 85 550 578 236 584 743 915
Cuts at: 4603 5095 5357 562
Size: 492 262 1738
Fragments arranged by size:
1738 915 743 584 578 550 492 350
262 236 85
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6533
SphI G_CATG'C
Cuts at: 1306 3586 4086 1306
Size: 2280 500 3753
Fragments arranged by size:
3753 2280 500
StuI AGG'CCT
Cuts at: 568 568
Size: 6533
StyI C'CwwG_G
Cuts at: 314 420 724 758 1020 1154 1922 2269 2855
Size: 106 304 34 262 134 768 347 586
Cuts at: 2855 2918 3667 4021 314
Size: 63 749 354 2826
Fragments arranged by size:
2826 768 749 586 354 347 304 262
134 106 63 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1081 1843 2020 2395 3148 4116 4249 4708 4917
Size: 762 177 375 753 968 133 459 209
Cuts at: 4917 1081
Size: 2697
Fragments arranged by size:
2697 968 762 753 459 375 209 177
133
TatI w'GTAC_w
Cuts at: 356 2717 6048 356
Size: 2361 3331 841
Fragments arranged by size:
3331 2361 841
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2447 2479 6050 6082 2447
Size: 32 3571 32 2898
Fragments arranged by size:
3571 2898 32 32
Tth111I GACn'n_nGTC
Cuts at: 4330 4330
Size: 6533
XbaI T'CTAG_A
Cuts at: 3564 3564
Size: 6533
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 439 2341 4028 439
Size: 1902 1687 2944
Fragments arranged by size:
2944 1902 1687
XhoI C'TCGA_G
Cuts at: 2945 2945
Size: 6533
XmaI C'CCGG_G
Cuts at: 3553 3553
Size: 6533
XmnI GAAnn'nnTTC
Cuts at: 2510 2510
Size: 6533
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI
BclI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NruI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AscI AvrII BaeI BbvCI BcgI
BfrBI BglI BmgBI Bpu10I BsiWI BsmI BsrGI BssHII
BstZ17I CspCI EcoRV FseI FspAI HpaI MfeI NdeI
NotI NsiI PacI PasI PmlI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI ZraI