Restriction map for
pF1KB0098
(Circular) (Six-base) MAPSORT of: pF1KB0098.seq Check: 6036 from: 1 to: 6110
pF1KB0098 6110 bp
With 182 enzymes: SgfI *
February 11, 2011 17:15 ..
AccI GT'mk_AC
Cuts at: 2435 3148 2435
Size: 713 5397
Acc65I G'GTAC_C
Cuts at: 281 3126 281
Size: 2845 3265
AclI AA'CG_TT
Cuts at: 5950 5950
Size: 6110
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 570 870 992 1265 1506 2022 3935 4367 5114
Size: 300 122 273 241 516 1913 432 747
Cuts at: 5114 5382 570
Size: 268 1298
Fragments arranged by size:
1913 1298 747 516 432 300 273 268
241 122
AflII C'TTAA_G
Cuts at: 1924 1924
Size: 6110
AflIII A'CryG_T
Cuts at: 1379 3462 4566 5543 1379
Size: 2083 1104 977 1946
Fragments arranged by size:
2083 1946 1104 977
AgeI A'CCGG_T
Cuts at: 4498 4498
Size: 6110
AhdI GACnn_n'nnGTC
Cuts at: 618 618
Size: 6110
AleI CACnn'nnGTG
Cuts at: 275 275
Size: 6110
AlwNI CAG_nnn'CTG
Cuts at: 1609 2739 3415 4982 1609
Size: 1130 676 1567 2737
Fragments arranged by size:
2737 1567 1130 676
ApaI G_GGCC'C
Cuts at: 775 1520 775
Size: 745 5365
ApaLI G'TGCA_C
Cuts at: 1476 4880 1476
Size: 3404 2706
ApoI r'AATT_y
Cuts at: 442 1880 2259 3114 5463 5719 442
Size: 1438 379 855 2349 256 833
Fragments arranged by size:
2349 1438 855 833 379 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6110
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6110
AvaI C'yCGr_G
Cuts at: 213 3130 213
Size: 2917 3193
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1120 1153 1120
Size: 33 6077
BamHI G'GATC_C
Cuts at: 3135 3135
Size: 6110
BanI G'GyrC_C
Cuts at: 281 1590 3126 3540 3788 3823 281
Size: 1309 1536 414 248 35 2568
Fragments arranged by size:
2568 1536 1309 414 248 35
BanII G_rGCy'C
Cuts at: 775 785 1222 1254 1412 1446 1520 2089 2749
Size: 10 437 32 158 34 74 569 660
Cuts at: 2749 2770 3124 4154 775
Size: 21 354 1030 2731
Fragments arranged by size:
2731 1030 660 569 437 354 158 74
34 32 21 10
BbeI G_GCGC'C
Cuts at: 1594 3544 3792 1594
Size: 1950 248 3912
Fragments arranged by size:
3912 1950 248
BbsI GAAGACnn'nnnn_
Cuts at: 327 876 1969 2582 327
Size: 549 1093 613 3855
Fragments arranged by size:
3855 1093 613 549
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3012 3046 3012
Size: 34 6076
BciVI GTATCCnnnnn_n'
Cuts at: 1030 4008 4769 6110 1030
Size: 2978 761 1341 1030
Fragments arranged by size:
2978 1341 1030 761
BclI T'GATC_A
Cuts at: 3630 3630
Size: 6110
BfrBI ATG'CAT
Cuts at: 1717 1717
Size: 6110
BglI GCCn_nnn'nGGC
Cuts at: 1433 1433
Size: 6110
BglII A'GATC_T
Cuts at: 2925 3625 5662 2925
Size: 700 2037 3373
Fragments arranged by size:
3373 2037 700
BlpI GC'TnA_GC
Cuts at: 3222 3222
Size: 6110
Bme1580I G_kGCm'C
Cuts at: 528 775 1480 1520 3735 3828 4884 528
Size: 247 705 40 2215 93 1056 1754
Fragments arranged by size:
2215 1754 1056 705 247 93 40
BmrI ACTGGGnnnn_n'
Cuts at: 3322 3403 3738 5882 3322
Size: 81 335 2144 3550
Fragments arranged by size:
3550 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4477 4477
Size: 6110
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2637 2761 2637
Size: 124 5986
Bpu10I CC'TnA_GC
Cuts at: 779 779
Size: 6110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2075 3286 4165 4657 4955 2075
Size: 1211 879 492 298 3230
Fragments arranged by size:
3230 1211 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3012 5814 3012
Size: 2802 3308
BsaAI yAC'GTr
Cuts at: 1380 1475 3463 1380
Size: 95 1988 4027
Fragments arranged by size:
4027 1988 95
BsaBI GATnn'nnATC
Cuts at: 6 943 3624 5661 6
Size: 937 2681 2037 455
Fragments arranged by size:
2681 2037 937 455
BsaHI Gr'CG_yC
Cuts at: 1591 3541 3789 4491 5987 1591
Size: 1950 248 702 1496 1714
Fragments arranged by size:
1950 1714 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1367 3299 3820 4498 4772 4919 5330 1367
Size: 1932 521 678 274 147 411 2147
Fragments arranged by size:
2147 1932 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1343 1373 1690 1720 2763 2793 1343
Size: 30 317 30 1043 30 4660
Fragments arranged by size:
4660 1043 317 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1741 2017 3069 1741
Size: 276 1052 4782
Fragments arranged by size:
4782 1052 276
BseYI C'CCAG_C
Cuts at: 135 311 3535 4293 4870 5366 135
Size: 176 3224 758 577 496 879
Fragments arranged by size:
3224 879 758 577 496 176
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 955 980 1412 1535 2808 622
Size: 333 25 432 123 1273 3924
Fragments arranged by size:
3924 1273 432 333 123 25
BsiEI CG_ry'CG
Cuts at: 104 3652 3698 4906 104
Size: 3548 46 1208 1308
Fragments arranged by size:
3548 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1412 1446 1480 1616 2089 2749 3124 3902 4092
Size: 34 34 136 473 660 375 778 190
Cuts at: 4092 4884 1412
Size: 792 2638
Fragments arranged by size:
2638 792 778 660 473 375 190 136
34 34
BsmI GAATG_Cn'
Cuts at: 1004 1061 1717 1719 1004
Size: 57 656 2 5395
Fragments arranged by size:
5395 656 57 2
Bsp1286I G_dGCh'C
Cuts at: 528 775 785 1222 1254 1412 1446 1480 1520
Size: 247 10 437 32 158 34 34 40
Cuts at: 1520 1616 2089 2749 2770 3124 3735 3828 3902
Size: 96 473 660 21 354 611 93 74
Cuts at: 3902 4092 4154 4884 528
Size: 190 62 730 1754
Fragments arranged by size:
1754 730 660 611 473 437 354 247
190 158 96 93 74 62 40 34
34 32 21 10
BspEI T'CCGG_A
Cuts at: 5330 5330
Size: 6110
BspHI T'CATG_A
Cuts at: 1103 2126 2865 2880 5286 1103
Size: 1023 739 15 2406 1927
Fragments arranged by size:
2406 1927 1023 739 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2150 3160 3374 3676 4057 2150
Size: 1010 214 302 381 4203
Fragments arranged by size:
4203 1010 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4402 4456 5940 6105 50
Size: 4352 54 1484 165 55
Fragments arranged by size:
4352 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2381 3046 4022 2381
Size: 665 976 4469
Fragments arranged by size:
4469 976 665
BsrFI r'CCGG_y
Cuts at: 114 4108 4289 4498 114
Size: 3994 181 209 1726
Fragments arranged by size:
3994 1726 209 181
BssSI C'ACGA_G
Cuts at: 4381 4739 4381
Size: 358 5752
BstAPI GCAn_nnn'nTGC
Cuts at: 532 3487 532
Size: 2955 3155
BstBI TT'CG_AA
Cuts at: 4471 4471
Size: 6110
BstEII G'GTnAC_C
Cuts at: 354 354
Size: 6110
BstXI CCAn_nnnn'nTGG
Cuts at: 1974 2847 1974
Size: 873 5237
BstYI r'GATC_y
Cuts at: 1 1448 1605 2925 3135 3602 3625 3960 4206
Size: 1447 157 1320 210 467 23 335 246
Cuts at: 4206 5218 5304 5316 5662 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1447 1320 1012 467 449 346 335 246
210 157 86 23 12
BtgI C'CryG_G
Cuts at: 107 2108 2550 107
Size: 2001 442 3667
Fragments arranged by size:
3667 2001 442
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4060 4241 4404 4060
Size: 181 163 5766
Fragments arranged by size:
5766 181 163
BtsI GCAGTG_nn'
Cuts at: 992 1220 1964 2916 3353 992
Size: 228 744 952 437 3749
Fragments arranged by size:
3749 952 744 437 228
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2771 2806 2771
Size: 35 6075
DraI TTT'AAA
Cuts at: 1271 3109 1271
Size: 1838 4272
DrdI GACnn_nn'nnGTC
Cuts at: 2314 3816 4674 2314
Size: 1502 858 3750
Fragments arranged by size:
3750 1502 858
EaeI y'GGCC_r
Cuts at: 1425 1676 2553 3695 3869 4260 4287 5632 1425
Size: 251 877 1142 174 391 27 1345 1903
Fragments arranged by size:
1903 1345 1142 877 391 251 174 27
EagI C'GGCC_G
Cuts at: 3695 3695
Size: 6110
EarI CTCTTCn'nnn_
Cuts at: 148 647 1584 1989 4133 4343 148
Size: 499 937 405 2144 210 1915
Fragments arranged by size:
2144 1915 937 499 405 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4318 4628 4774 5920 4318
Size: 310 146 1146 4508
Fragments arranged by size:
4508 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1410 1444 2087 2747 3122 1410
Size: 34 643 660 375 4398
Fragments arranged by size:
4398 660 643 375 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 570 870 992 1265 1506 2022 2637 2761 3935
Size: 300 122 273 241 516 615 124 1174
Cuts at: 3935 4367 5114 5382 570
Size: 432 747 268 1298
Fragments arranged by size:
1298 1174 747 615 516 432 300 273
268 241 124 122
EcoNI CCTnn'n_nnAGG
Cuts at: 224 2761 2882 224
Size: 2537 121 3452
Fragments arranged by size:
3452 2537 121
EcoO109I rG'GnC_Cy
Cuts at: 217 1517 1940 2139 2754 3249 217
Size: 1300 423 199 615 495 3078
Fragments arranged by size:
3078 1300 615 495 423 199
EcoRI G'AATT_C
Cuts at: 3114 3114
Size: 6110
EcoRV GAT'ATC
Cuts at: 1373 1373
Size: 6110
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1785 1939 4129 1785
Size: 154 2190 3766
Fragments arranged by size:
3766 2190 154
FseI GG_CCGG'CC
Cuts at: 118 118
Size: 6110
FspI TGC'GCA
Cuts at: 3891 3891
Size: 6110
HaeII r_GCGC'y
Cuts at: 1594 3544 3792 4814 5793 1594
Size: 1950 248 1022 979 1911
Fragments arranged by size:
1950 1911 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 604 636 646 678 923 955 1024 1056 1314
Size: 32 10 32 245 32 69 32 258
Cuts at: 1314 1346 1433 1465 1888 1920 1927 1959 2493
Size: 32 87 32 423 32 7 32 534
Cuts at: 2493 2525 2610 2642 2791 2823 2879 2911 3064
Size: 32 85 32 149 32 56 32 153
Cuts at: 3064 3096 3952 3982 3984 4014 604
Size: 32 856 30 2 30 2700
Fragments arranged by size:
2700 856 534 423 258 245 153 149
87 85 69 56 32 32 32 32
32 32 32 32 32 32 32 32
32 30 30 10 7 2
HincII GTy'rAC
Cuts at: 789 822 3149 789
Size: 33 2327 3750
Fragments arranged by size:
3750 2327 33
HpaI GTT'AAC
Cuts at: 822 822
Size: 6110
KasI G'GCGC_C
Cuts at: 1590 3540 3788 1590
Size: 1950 248 3912
Fragments arranged by size:
3912 1950 248
KpnI G_GTAC'C
Cuts at: 285 3130 285
Size: 2845 3265
MluI A'CGCG_T
Cuts at: 5543 5543
Size: 6110
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 387 1357 1955 4781 4965 387
Size: 970 598 2826 184 1532
Fragments arranged by size:
2826 1532 970 598 184
MscI TGG'CCA
Cuts at: 1427 1678 2555 3871 1427
Size: 251 877 1316 3666
Fragments arranged by size:
3666 1316 877 251
MslI CAynn'nnrTG
Cuts at: 275 434 2048 2845 4099 4226 275
Size: 159 1614 797 1254 127 2159
Fragments arranged by size:
2159 1614 1254 797 159 127
MspA1I CmG'CkG
Cuts at: 123 129 255 1086 1152 1992 2959 3221 3535
Size: 6 126 831 66 840 967 262 314
Cuts at: 3535 3895 4908 5153 123
Size: 360 1013 245 1080
Fragments arranged by size:
1080 1013 967 840 831 360 314 262
245 126 66 6
NaeI GCC'GGC
Cuts at: 116 4291 116
Size: 4175 1935
NarI GG'CG_CC
Cuts at: 1591 3541 3789 1591
Size: 1950 248 3912
Fragments arranged by size:
3912 1950 248
NcoI C'CATG_G
Cuts at: 107 2108 2550 107
Size: 2001 442 3667
Fragments arranged by size:
3667 2001 442
NgoMIV G'CCGG_C
Cuts at: 114 4289 114
Size: 4175 1935
NsiI A_TGCA'T
Cuts at: 1719 1719
Size: 6110
NspI r_CATG'y
Cuts at: 2298 3030 3163 3663 4570 2298
Size: 732 133 500 907 3838
Fragments arranged by size:
3838 907 732 500 133
PasI CC'CwG_GG
Cuts at: 2232 2763 2232
Size: 531 5579
PciI A'CATG_T
Cuts at: 4566 4566
Size: 6110
PfoI T'CCnGG_A
Cuts at: 1694 1694
Size: 6110
PmeI GTTT'AAAC
Cuts at: 3109 3109
Size: 6110
PmlI CAC'GTG
Cuts at: 1380 1380
Size: 6110
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5249 5281 5249
Size: 32 6078
PpuMI rG'GwC_Cy
Cuts at: 217 1940 2139 217
Size: 1723 199 4188
Fragments arranged by size:
4188 1723 199
PshAI GACnn'nnGTC
Cuts at: 361 361
Size: 6110
PspOMI G'GGCC_C
Cuts at: 771 1516 771
Size: 745 5365
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1781 1813 1781
Size: 32 6078
PstI C_TGCA'G
Cuts at: 1215 2201 2666 2952 3157 3842 1215
Size: 986 465 286 205 685 3483
Fragments arranged by size:
3483 986 685 465 286 205
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6110
PvuII CAG'CTG
Cuts at: 1152 2959 3535 3895 1152
Size: 1807 576 360 3367
Fragments arranged by size:
3367 1807 576 360
RsrII CG'GwC_CG
Cuts at: 4305 4305
Size: 6110
SacI G_AGCT'C
Cuts at: 1412 1446 2089 2749 3124 1412
Size: 34 643 660 375 4398
Fragments arranged by size:
4398 660 643 375 34
SalI G'TCGA_C
Cuts at: 3147 3147
Size: 6110
SapI GCTCTTCn'nnn_
Cuts at: 4133 4343 4133
Size: 210 5900
SbfI CC_TGCA'GG
Cuts at: 3157 3157
Size: 6110
ScaI AGT'ACT
Cuts at: 5627 5627
Size: 6110
SfcI C'TryA_G
Cuts at: 33 1211 2197 2662 2948 3153 3838 4831 5022
Size: 1178 986 465 286 205 685 993 191
Cuts at: 5022 33
Size: 1121
Fragments arranged by size:
1178 1121 993 986 685 465 286 205
191
SfoI GGC'GCC
Cuts at: 1592 3542 3790 1592
Size: 1950 248 3912
Fragments arranged by size:
3912 1950 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6110
SmaI CCC'GGG
Cuts at: 215 3132 215
Size: 2917 3193
SmlI C'TyrA_G
Cuts at: 1924 2090 3265 4180 4672 4934 1924
Size: 166 1175 915 492 262 3100
Fragments arranged by size:
3100 1175 915 492 262 166
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6110
SphI G_CATG'C
Cuts at: 3030 3163 3663 3030
Size: 133 500 5477
Fragments arranged by size:
5477 500 133
SspI AAT'ATT
Cuts at: 1541 1541
Size: 6110
StuI AGG'CCT
Cuts at: 866 866
Size: 6110
StyI C'CwwG_G
Cuts at: 107 926 1222 1673 2108 2373 2550 3035 3244
Size: 819 296 451 435 265 177 485 209
Cuts at: 3244 3598 107
Size: 354 2619
Fragments arranged by size:
2619 819 485 451 435 354 296 265
209 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1876 2060 2444 3693 3826 4285 4494 1876
Size: 184 384 1249 133 459 209 3492
Fragments arranged by size:
3492 1249 459 384 209 184 133
TatI w'GTAC_w
Cuts at: 684 2606 5625 684
Size: 1922 3019 1169
Fragments arranged by size:
3019 1922 1169
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1343 1375 2763 2795 5627 5659 1343
Size: 32 1388 32 2832 32 1794
Fragments arranged by size:
2832 1794 1388 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 921 2431 3907 921
Size: 1510 1476 3124
Fragments arranged by size:
3124 1510 1476
XbaI T'CTAG_A
Cuts at: 3141 3141
Size: 6110
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1410 3605 1410
Size: 2195 3915
XmaI C'CCGG_G
Cuts at: 213 3130 213
Size: 2917 3193
XmnI GAAnn'nnTTC
Cuts at: 1717 1768 2035 1717
Size: 51 267 5792
Fragments arranged by size:
5792 267 51
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AlfI AloI AscI AvrII BbvCI
BmgBI BmtI BsiWI BsmBI BsrGI BssHII BstZ17I Bsu36I
ClaI DraIII FspAI HindIII MfeI NdeI NheI NotI
NruI PacI PflMI PsiI PspXI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI