Restriction map for
pF1KB0150
(Circular) (Six-base) MAPSORT of: pF1KB0150.seq Check: 3839 from: 1 to: 7505
pF1KB0150 7505 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 2137 4543 2137
Size: 2406 5099
Acc65I G'GTAC_C
Cuts at: 4521 4521
Size: 7505
AclI AA'CG_TT
Cuts at: 512 7345 512
Size: 6833 672
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1702 1907 3097 3670 5330 5762 6509 6777 1702
Size: 205 1190 573 1660 432 747 268 2430
Fragments arranged by size:
2430 1660 1190 747 573 432 268 205
AflIII A'CryG_T
Cuts at: 4857 5961 6938 4857
Size: 1104 977 5424
Fragments arranged by size:
5424 1104 977
AgeI A'CCGG_T
Cuts at: 5893 5893
Size: 7505
AhdI GACnn_n'nnGTC
Cuts at: 3243 3243
Size: 7505
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2767 2767
Size: 7505
AlwNI CAG_nnn'CTG
Cuts at: 379 1329 2040 2278 2431 3810 4810 6377 379
Size: 950 711 238 153 1379 1000 1567 1507
Fragments arranged by size:
1567 1507 1379 1000 950 711 238 153
ApaLI G'TGCA_C
Cuts at: 6275 6275
Size: 7505
ApoI r'AATT_y
Cuts at: 273 344 616 1377 2171 2699 2763 2898 3372
Size: 71 272 761 794 528 64 135 474
Cuts at: 3372 4509 6858 7114 273
Size: 1137 2349 256 664
Fragments arranged by size:
2349 1137 794 761 664 528 474 272
256 135 71 64
AseI AT'TA_AT
Cuts at: 20 2449 3797 20
Size: 2429 1348 3728
Fragments arranged by size:
3728 2429 1348
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7505
AvaI C'yCGr_G
Cuts at: 4393 4525 4393
Size: 132 7373
BamHI G'GATC_C
Cuts at: 4530 4530
Size: 7505
BanI G'GyrC_C
Cuts at: 2590 2970 3495 3789 4521 4935 5183 5218 2590
Size: 380 525 294 732 414 248 35 4877
Fragments arranged by size:
4877 732 525 414 380 294 248 35
BanII G_rGCy'C
Cuts at: 1588 1883 2069 2317 2682 3342 4078 4519 5549
Size: 295 186 248 365 660 736 441 1030
Cuts at: 5549 1588
Size: 3544
Fragments arranged by size:
3544 1030 736 660 441 365 295 248
186
BbeI G_GCGC'C
Cuts at: 2594 3499 4939 5187 2594
Size: 905 1440 248 4912
Fragments arranged by size:
4912 1440 905 248
BbvCI CC'TCA_GC
Cuts at: 1726 2852 1726
Size: 1126 6379
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 427 461 427
Size: 34 7471
BciVI GTATCCnnnnn_n'
Cuts at: 423 951 2088 5403 6164 7505 423
Size: 528 1137 3315 761 1341 423
Fragments arranged by size:
3315 1341 1137 761 528 423
BclI T'GATC_A
Cuts at: 2055 5025 2055
Size: 2970 4535
BfrBI ATG'CAT
Cuts at: 3520 3864 3520
Size: 344 7161
BglI GCCn_nnn'nGGC
Cuts at: 1324 3095 4150 1324
Size: 1771 1055 4679
Fragments arranged by size:
4679 1771 1055
BglII A'GATC_T
Cuts at: 5020 7057 5020
Size: 2037 5468
BlpI GC'TnA_GC
Cuts at: 4617 4617
Size: 7505
Bme1580I G_kGCm'C
Cuts at: 1739 2973 5130 5223 6279 1739
Size: 1234 2157 93 1056 2965
Fragments arranged by size:
2965 2157 1234 1056 93
BmgBI CAC'GTC
Cuts at: 950 950
Size: 7505
BmrI ACTGGGnnnn_n'
Cuts at: 1613 1798 2749 4064 4717 4798 5133 7277 1613
Size: 185 951 1315 653 81 335 2144 1841
Fragments arranged by size:
2144 1841 1315 951 653 335 185 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3989 5872 3989
Size: 1883 5622
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 126 1610 1946 2981 3269 3406 3505 3794 126
Size: 1484 336 1035 288 137 99 289 3837
Fragments arranged by size:
3837 1484 1035 336 289 288 137 99
Bpu10I CC'TnA_GC
Cuts at: 1726 2076 2251 2852 1726
Size: 350 175 601 6379
Fragments arranged by size:
6379 601 350 175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 2140 2425 3444 4681 5560 6052 6350 487
Size: 1653 285 1019 1237 879 492 298 1642
Fragments arranged by size:
1653 1642 1237 1019 879 492 298 285
BsaI GGTCTCn'nnnn_
Cuts at: 1749 7209 1749
Size: 5460 2045
BsaAI yAC'GTr
Cuts at: 4858 4858
Size: 7505
BsaBI GATnn'nnATC
Cuts at: 6 5019 7056 6
Size: 5013 2037 455
Fragments arranged by size:
5013 2037 455
BsaHI Gr'CG_yC
Cuts at: 2591 3244 3496 4936 5184 5886 7382 2591
Size: 653 252 1440 248 702 1496 2714
Fragments arranged by size:
2714 1496 1440 702 653 252 248
BsaWI w'CCGG_w
Cuts at: 1257 4694 5215 5893 6167 6314 6725 1257
Size: 3437 521 678 274 147 411 2037
Fragments arranged by size:
3437 2037 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1037 1067 2092 2122 1037
Size: 30 1025 30 6420
Fragments arranged by size:
6420 1025 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 757 1469 2488 2542 3182 3403 757
Size: 712 1019 54 640 221 4859
Fragments arranged by size:
4859 1019 712 640 221 54
BseYI C'CCAG_C
Cuts at: 190 450 992 3057 3082 4140 4211 4930 5688
Size: 260 542 2065 25 1058 71 719 758
Cuts at: 5688 6265 6761 190
Size: 577 496 934
Fragments arranged by size:
2065 1058 934 758 719 577 542 496
260 71 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 1136 1353 3673 418
Size: 718 217 2320 4250
Fragments arranged by size:
4250 2320 718 217
BsiEI CG_ry'CG
Cuts at: 104 1967 5047 5093 6301 104
Size: 1863 3080 46 1208 1308
Fragments arranged by size:
3080 1863 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2713 3342 4519 5297 5487 6279 2713
Size: 629 1177 778 190 792 3939
Fragments arranged by size:
3939 1177 792 778 629 190
BsmI GAATG_Cn'
Cuts at: 1102 1647 2337 1102
Size: 545 690 6270
Fragments arranged by size:
6270 690 545
BsmBI CGTCTCn'nnnn_
Cuts at: 956 956
Size: 7505
Bsp1286I G_dGCh'C
Cuts at: 1588 1739 1883 2069 2317 2682 2713 2973 3342
Size: 151 144 186 248 365 31 260 369
Cuts at: 3342 4078 4519 5130 5223 5297 5487 5549 6279
Size: 736 441 611 93 74 190 62 730
Cuts at: 6279 1588
Size: 2814
Fragments arranged by size:
2814 736 730 611 441 369 365 260
248 190 186 151 144 93 74 62
31
BspEI T'CCGG_A
Cuts at: 1257 6725 1257
Size: 5468 2037
BspHI T'CATG_A
Cuts at: 750 1077 3125 6681 750
Size: 327 2048 3556 1574
Fragments arranged by size:
3556 2048 1574 327
BspMI ACCTGCnnnn'nnnn_
Cuts at: 638 1415 1964 2735 4389 4555 4769 5071 5452
Size: 777 549 771 1654 166 214 302 381
Cuts at: 5452 638
Size: 2691
Fragments arranged by size:
2691 1654 777 771 549 381 302 214
166
BsrBI CCG'CTC
Cuts at: 50 5797 5851 7335 7500 50
Size: 5747 54 1484 165 55
Fragments arranged by size:
5747 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2462 2782 3747 5417 2462
Size: 320 965 1670 4550
Fragments arranged by size:
4550 1670 965 320
BsrFI r'CCGG_y
Cuts at: 2307 3026 5503 5684 5893 2307
Size: 719 2477 181 209 3919
Fragments arranged by size:
3919 2477 719 209 181
BsrGI T'GTAC_A
Cuts at: 4256 4256
Size: 7505
BssSI C'ACGA_G
Cuts at: 2580 5776 6134 2580
Size: 3196 358 3951
Fragments arranged by size:
3951 3196 358
BstAPI GCAn_nnn'nTGC
Cuts at: 379 4882 379
Size: 4503 3002
BstBI TT'CG_AA
Cuts at: 5866 5866
Size: 7505
BstEII G'GTnAC_C
Cuts at: 2494 2494
Size: 7505
BstXI CCAn_nnnn'nTGG
Cuts at: 4450 4450
Size: 7505
BstYI r'GATC_y
Cuts at: 1 658 930 1260 2427 3444 3564 4530 4997
Size: 657 272 330 1167 1017 120 966 467
Cuts at: 4997 5020 5355 5601 6613 6699 6711 7057 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1167 1017 1012 966 657 467 449 346
335 330 272 246 120 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1481 3405 3634 1481
Size: 1924 229 5352
Fragments arranged by size:
5352 1924 229
BtgI C'CryG_G
Cuts at: 3211 3581 3641 4286 4385 3211
Size: 370 60 645 99 6331
Fragments arranged by size:
6331 645 370 99 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1561 3676 5455 5636 5799 1561
Size: 2115 1779 181 163 3267
Fragments arranged by size:
3267 2115 1779 181 163
BtsI GCAGTG_nn'
Cuts at: 796 1341 1980 2514 2692 4748 796
Size: 545 639 534 178 2056 3553
Fragments arranged by size:
3553 2056 639 545 534 178
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3917 3952 4284 4319 3917
Size: 35 332 35 7103
Fragments arranged by size:
7103 332 35 35
DraI TTT'AAA
Cuts at: 4504 4504
Size: 7505
DrdI GACnn_nn'nnGTC
Cuts at: 3114 5211 6069 3114
Size: 2097 858 4550
Fragments arranged by size:
4550 2097 858
EaeI y'GGCC_r
Cuts at: 1964 2216 3028 3096 5090 5264 5655 5682 7027
Size: 252 812 68 1994 174 391 27 1345
Cuts at: 7027 1964
Size: 2442
Fragments arranged by size:
2442 1994 1345 812 391 252 174 68
27
EagI C'GGCC_G
Cuts at: 1964 5090 1964
Size: 3126 4379
EarI CTCTTCn'nnn_
Cuts at: 557 734 864 1156 5528 5738 557
Size: 177 130 292 4372 210 2324
Fragments arranged by size:
4372 2324 292 210 177 130
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 699 4208 5713 6023 6169 7315 699
Size: 3509 1505 310 146 1146 889
Fragments arranged by size:
3509 1505 1146 889 310 146
EcoICRI GAG'CTC
Cuts at: 3340 4517 3340
Size: 1177 6328
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 126 1610 1702 1907 1946 2981 3097 3269 3406
Size: 1484 92 205 39 1035 116 172 137
Cuts at: 3406 3505 3670 3794 5330 5762 6509 6777 126
Size: 99 165 124 1536 432 747 268 854
Fragments arranged by size:
1536 1484 1035 854 747 432 268 205
172 165 137 124 116 99 92 39
EcoNI CCTnn'n_nnAGG
Cuts at: 1729 2254 2855 4043 4370 1729
Size: 525 601 1188 327 4864
Fragments arranged by size:
4864 1188 601 525 327
EcoO109I rG'GnC_Cy
Cuts at: 454 471 999 1326 1722 3166 4152 4390 4644
Size: 17 528 327 396 1444 986 238 254
Cuts at: 4644 454
Size: 3315
Fragments arranged by size:
3315 1444 986 528 396 327 254 238
17
EcoRI G'AATT_C
Cuts at: 4509 4509
Size: 7505
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5524 5524
Size: 7505
FspI TGC'GCA
Cuts at: 1034 5286 1034
Size: 4252 3253
HaeII r_GCGC'y
Cuts at: 2594 3499 4939 5187 6209 7188 2594
Size: 905 1440 248 1022 979 2911
Fragments arranged by size:
2911 1440 1022 979 905 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 400 432 528 560 829 861 936 968 1059
Size: 32 96 32 269 32 75 32 91
Cuts at: 1059 1091 2856 2888 3229 3261 3617 3649 5347
Size: 32 1765 32 341 32 356 32 1698
Cuts at: 5347 5377 5379 5409 400
Size: 30 2 30 2496
Fragments arranged by size:
2496 1765 1698 356 341 269 96 91
75 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2225 2610 4544 2225
Size: 385 1934 5186
Fragments arranged by size:
5186 1934 385
HindIII A'AGCT_T
Cuts at: 1641 1842 1641
Size: 201 7304
HpaI GTT'AAC
Cuts at: 2225 2225
Size: 7505
KasI G'GCGC_C
Cuts at: 2590 3495 4935 5183 2590
Size: 905 1440 248 4912
Fragments arranged by size:
4912 1440 905 248
KpnI G_GTAC'C
Cuts at: 4525 4525
Size: 7505
MfeI C'AATT_G
Cuts at: 702 702
Size: 7505
MluI A'CGCG_T
Cuts at: 6938 6938
Size: 7505
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 688 2072 2655 3497 4184 6176 6360 688
Size: 1384 583 842 687 1992 184 1833
Fragments arranged by size:
1992 1833 1384 842 687 583 184
MscI TGG'CCA
Cuts at: 2218 3098 5266 2218
Size: 880 2168 4457
Fragments arranged by size:
4457 2168 880
MslI CAynn'nnrTG
Cuts at: 1076 1558 2920 3073 4252 4279 5494 5621 1076
Size: 482 1362 153 1179 27 1215 127 2960
Fragments arranged by size:
2960 1362 1215 1179 482 153 127 27
MspA1I CmG'CkG
Cuts at: 379 1916 2037 2650 3316 3354 3591 4446 4616
Size: 1537 121 613 666 38 237 855 170
Cuts at: 4616 4930 5290 6303 6548 379
Size: 314 360 1013 245 1336
Fragments arranged by size:
1537 1336 1013 855 666 613 360 314
245 237 170 121 38
NaeI GCC'GGC
Cuts at: 5686 5686
Size: 7505
NarI GG'CG_CC
Cuts at: 2591 3496 4936 5184 2591
Size: 905 1440 248 4912
Fragments arranged by size:
4912 1440 905 248
NcoI C'CATG_G
Cuts at: 3211 3581 4286 4385 3211
Size: 370 705 99 6331
Fragments arranged by size:
6331 705 370 99
NdeI CA'TA_TG
Cuts at: 1220 1220
Size: 7505
NgoMIV G'CCGG_C
Cuts at: 5684 5684
Size: 7505
NsiI A_TGCA'T
Cuts at: 3522 3866 3522
Size: 344 7161
NspI r_CATG'y
Cuts at: 601 1416 1467 4206 4257 4558 5058 5965 601
Size: 815 51 2739 51 301 500 907 2141
Fragments arranged by size:
2739 2141 907 815 500 301 51 51
PasI CC'CwG_GG
Cuts at: 3540 3540
Size: 7505
PciI A'CATG_T
Cuts at: 5961 5961
Size: 7505
PflMI CCAn_nnn'nTGG
Cuts at: 4231 4231
Size: 7505
PfoI T'CCnGG_A
Cuts at: 1542 1923 2277 3344 1542
Size: 381 354 1067 5703
Fragments arranged by size:
5703 1067 381 354
PmeI GTTT'AAAC
Cuts at: 4504 4504
Size: 7505
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1501 1533 6644 6676 1501
Size: 32 5111 32 2330
Fragments arranged by size:
5111 2330 32 32
PpuMI rG'GwC_Cy
Cuts at: 999 3166 4390 999
Size: 2167 1224 4114
Fragments arranged by size:
4114 2167 1224
PstI C_TGCA'G
Cuts at: 1638 2036 3315 4552 5237 1638
Size: 398 1279 1237 685 3906
Fragments arranged by size:
3906 1279 1237 685 398
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7505
PvuII CAG'CTG
Cuts at: 379 1916 2037 2650 3316 3354 4446 4930 5290
Size: 1537 121 613 666 38 1092 484 360
Cuts at: 5290 379
Size: 2594
Fragments arranged by size:
2594 1537 1092 666 613 484 360 121
38
RsrII CG'GwC_CG
Cuts at: 5700 5700
Size: 7505
SacI G_AGCT'C
Cuts at: 3342 4519 3342
Size: 1177 6328
SalI G'TCGA_C
Cuts at: 4542 4542
Size: 7505
SanDI GG'GwC_CC
Cuts at: 4390 4390
Size: 7505
SapI GCTCTTCn'nnn_
Cuts at: 557 1156 5528 5738 557
Size: 599 4372 210 2324
Fragments arranged by size:
4372 2324 599 210
SbfI CC_TGCA'GG
Cuts at: 1638 4552 1638
Size: 2914 4591
ScaI AGT'ACT
Cuts at: 1085 7022 1085
Size: 5937 1568
SexAI A'CCwGG_T
Cuts at: 1001 1001
Size: 7505
SfcI C'TryA_G
Cuts at: 33 419 644 1634 2032 3233 3311 4548 5233
Size: 386 225 990 398 1201 78 1237 685
Cuts at: 5233 6226 6417 33
Size: 993 191 1121
Fragments arranged by size:
1237 1201 1121 993 990 685 398 386
225 191 78
SfoI GGC'GCC
Cuts at: 2592 3497 4937 5185 2592
Size: 905 1440 248 4912
Fragments arranged by size:
4912 1440 905 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7505
SmaI CCC'GGG
Cuts at: 4527 4527
Size: 7505
SmlI C'TyrA_G
Cuts at: 466 2155 2404 3459 4660 5575 6067 6329 466
Size: 1689 249 1055 1201 915 492 262 1642
Fragments arranged by size:
1689 1642 1201 1055 915 492 262 249
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7505
SphI G_CATG'C
Cuts at: 1416 1467 4558 5058 1416
Size: 51 3091 500 3863
Fragments arranged by size:
3863 3091 500 51
SspI AAT'ATT
Cuts at: 4495 4495
Size: 7505
StuI AGG'CCT
Cuts at: 3771 3771
Size: 7505
StyI C'CwwG_G
Cuts at: 936 1570 2892 3036 3211 3581 4286 4385 4639
Size: 634 1322 144 175 370 705 99 254
Cuts at: 4639 4993 936
Size: 354 3448
Fragments arranged by size:
3448 1322 705 634 370 354 254 175
144 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1632 5088 5221 5680 5889 1632
Size: 3456 133 459 209 3248
Fragments arranged by size:
3456 3248 459 209 133
TatI w'GTAC_w
Cuts at: 1083 4256 7020 1083
Size: 3173 2764 1568
Fragments arranged by size:
3173 2764 1568
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1037 1069 2273 2305 2555 2587 7022 7054 1037
Size: 32 1204 32 250 32 4435 32 1488
Fragments arranged by size:
4435 1488 1204 250 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5302 5302
Size: 7505
XbaI T'CTAG_A
Cuts at: 4536 4536
Size: 7505
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2963 3480 5000 114
Size: 2849 517 1520 2619
Fragments arranged by size:
2849 2619 1520 517
XmaI C'CCGG_G
Cuts at: 4525 4525
Size: 7505
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BbsI BmtI BsiWI BssHII BstZ17I ClaI
DraIII EcoRV FseI FspAI NheI NotI NruI PacI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SfiI
SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI