Restriction map for
pF1KB0166
(Circular) (Six-base) MAPSORT of: pF1KB0166.seq Check: 3414 from: 1 to: 6581
pF1KB0166 6581 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AatII G_ACGT'C
Cuts at: 1977 1977
Size: 6581
AccI GT'mk_AC
Cuts at: 1805 3619 1805
Size: 1814 4767
Acc65I G'GTAC_C
Cuts at: 142 3597 142
Size: 3455 3126
AclI AA'CG_TT
Cuts at: 355 6421 355
Size: 6066 515
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 517 998 1075 1325 1568 2294 2485 3032
Size: 92 481 77 250 243 726 191 547
Cuts at: 3032 3325 4406 4838 5585 5853 425
Size: 293 1081 432 747 268 1153
Fragments arranged by size:
1153 1081 747 726 547 481 432 293
268 250 243 191 92 77
AfeI AGC'GCT
Cuts at: 2767 3106 2767
Size: 339 6242
AflII C'TTAA_G
Cuts at: 2566 2566
Size: 6581
AflIII A'CryG_T
Cuts at: 3933 5037 6014 3933
Size: 1104 977 4500
Fragments arranged by size:
4500 1104 977
AgeI A'CCGG_T
Cuts at: 4969 4969
Size: 6581
AleI CACnn'nnGTG
Cuts at: 560 560
Size: 6581
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2062 2094 2062
Size: 32 6549
AlwNI CAG_nnn'CTG
Cuts at: 1267 1402 1591 1831 2676 3144 3886 5453 1267
Size: 135 189 240 845 468 742 1567 2395
Fragments arranged by size:
2395 1567 845 742 468 240 189 135
ApaI G_GGCC'C
Cuts at: 163 182 163
Size: 19 6562
ApaLI G'TGCA_C
Cuts at: 5351 5351
Size: 6581
ApoI r'AATT_y
Cuts at: 899 1408 2793 2886 3585 5934 6190 899
Size: 509 1385 93 699 2349 256 1290
Fragments arranged by size:
2349 1385 1290 699 509 256 93
AseI AT'TA_AT
Cuts at: 20 2930 20
Size: 2910 3671
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6581
AvaI C'yCGr_G
Cuts at: 138 175 3601 138
Size: 37 3426 3118
Fragments arranged by size:
3426 3118 37
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2716 2749 2716
Size: 33 6548
BamHI G'GATC_C
Cuts at: 3606 3606
Size: 6581
BanI G'GyrC_C
Cuts at: 142 1471 3597 4011 4259 4294 142
Size: 1329 2126 414 248 35 2429
Fragments arranged by size:
2429 2126 1329 414 248 35
BanII G_rGCy'C
Cuts at: 163 182 817 1053 1739 3595 4625 163
Size: 19 635 236 686 1856 1030 2119
Fragments arranged by size:
2119 1856 1030 686 635 236 19
BbeI G_GCGC'C
Cuts at: 4015 4263 4015
Size: 248 6333
BbsI GAAGACnn'nnnn_
Cuts at: 767 1313 1565 1919 767
Size: 546 252 354 5429
Fragments arranged by size:
5429 546 354 252
BciVI GTATCCnnnnn_n'
Cuts at: 2196 4479 5240 6581 2196
Size: 2283 761 1341 2196
Fragments arranged by size:
2283 2196 1341 761
BclI T'GATC_A
Cuts at: 2282 2787 4101 2282
Size: 505 1314 4762
Fragments arranged by size:
4762 1314 505
BfrBI ATG'CAT
Cuts at: 1027 1027
Size: 6581
BglI GCCn_nnn'nGGC
Cuts at: 849 849
Size: 6581
BglII A'GATC_T
Cuts at: 2640 3416 4096 6133 2640
Size: 776 680 2037 3088
Fragments arranged by size:
3088 2037 776 680
BlpI GC'TnA_GC
Cuts at: 280 3095 3693 280
Size: 2815 598 3168
Fragments arranged by size:
3168 2815 598
Bme1580I G_kGCm'C
Cuts at: 163 182 4206 4299 5355 163
Size: 19 4024 93 1056 1389
Fragments arranged by size:
4024 1389 1056 93 19
BmrI ACTGGGnnnn_n'
Cuts at: 238 1412 2772 3793 3874 4209 6353 238
Size: 1174 1360 1021 81 335 2144 466
Fragments arranged by size:
2144 1360 1174 1021 466 335 81
BmtI G_CTAG'C
Cuts at: 206 206
Size: 6581
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4948 4948
Size: 6581
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 579 2239 2632 2746 2845 579
Size: 1660 393 114 99 4315
Fragments arranged by size:
4315 1660 393 114 99
Bpu10I CC'TnA_GC
Cuts at: 816 816
Size: 6581
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2050 2259 2287 3045 3757 4636 5128 5426 2050
Size: 209 28 758 712 879 492 298 3205
Fragments arranged by size:
3205 879 758 712 492 298 209 28
BsaI GGTCTCn'nnnn_
Cuts at: 1615 3118 6285 1615
Size: 1503 3167 1911
Fragments arranged by size:
3167 1911 1503
BsaAI yAC'GTr
Cuts at: 3934 3934
Size: 6581
BsaBI GATnn'nnATC
Cuts at: 6 4095 6132 6
Size: 4089 2037 455
Fragments arranged by size:
4089 2037 455
BsaHI Gr'CG_yC
Cuts at: 1974 4012 4260 4962 6458 1974
Size: 2038 248 702 1496 2097
Fragments arranged by size:
2097 2038 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3465 3770 4291 4969 5243 5390 5801 3465
Size: 305 521 678 274 147 411 4245
Fragments arranged by size:
4245 678 521 411 305 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1442 1472 2062 2092 1442
Size: 30 590 30 5931
Fragments arranged by size:
5931 590 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2242 2642 2242
Size: 400 6181
BseYI C'CCAG_C
Cuts at: 114 1824 4006 4764 5341 5837 114
Size: 1710 2182 758 577 496 858
Fragments arranged by size:
2182 1710 858 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 3173 772
Size: 2401 4180
BsiEI CG_ry'CG
Cuts at: 104 263 267 4123 4169 5377 104
Size: 159 4 3856 46 1208 1308
Fragments arranged by size:
3856 1308 1208 159 46 4
BsiHKAI G_wGCw'C
Cuts at: 251 817 1393 1739 3595 4373 4563 5355 251
Size: 566 576 346 1856 778 190 792 1477
Fragments arranged by size:
1856 1477 792 778 576 566 346 190
BsmI GAATG_Cn'
Cuts at: 3443 3443
Size: 6581
Bsp1286I G_dGCh'C
Cuts at: 163 182 251 817 1053 1393 1739 3595 4206
Size: 19 69 566 236 340 346 1856 611
Cuts at: 4206 4299 4373 4563 4625 5355 163
Size: 93 74 190 62 730 1389
Fragments arranged by size:
1856 1389 730 611 566 346 340 236
190 93 74 69 62 19
BspEI T'CCGG_A
Cuts at: 5801 5801
Size: 6581
BspHI T'CATG_A
Cuts at: 2901 5757 2901
Size: 2856 3725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3488 3631 3845 4147 4528 3488
Size: 143 214 302 381 5541
Fragments arranged by size:
5541 381 302 214 143
BsrBI CCG'CTC
Cuts at: 50 4873 4927 6411 6576 50
Size: 4823 54 1484 165 55
Fragments arranged by size:
4823 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4493 4493
Size: 6581
BsrFI r'CCGG_y
Cuts at: 258 266 4579 4760 4969 258
Size: 8 4313 181 209 1870
Fragments arranged by size:
4313 1870 209 181 8
BssSI C'ACGA_G
Cuts at: 4852 5210 4852
Size: 358 6223
BstAPI GCAn_nnn'nTGC
Cuts at: 840 858 3958 840
Size: 18 3100 3463
Fragments arranged by size:
3463 3100 18
BstBI TT'CG_AA
Cuts at: 4942 4942
Size: 6581
BstYI r'GATC_y
Cuts at: 1 361 652 1084 2640 3416 3606 4073 4096
Size: 360 291 432 1556 776 190 467 23
Cuts at: 4096 4431 4677 5689 5775 5787 6133 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1556 1012 776 467 449 432 360 346
335 291 246 190 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 597 845 1859 597
Size: 248 1014 5319
Fragments arranged by size:
5319 1014 248
BtgI C'CryG_G
Cuts at: 130 1837 2398 130
Size: 1707 561 4313
Fragments arranged by size:
4313 1707 561
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3187 4531 4712 4875 3187
Size: 1344 181 163 4893
Fragments arranged by size:
4893 1344 181 163
BtsI GCAGTG_nn'
Cuts at: 755 3824 755
Size: 3069 3512
ClaI AT'CG_AT
Cuts at: 2534 2534
Size: 6581
DraI TTT'AAA
Cuts at: 3434 3580 3434
Size: 146 6435
DraIII CAC_nnn'GTG
Cuts at: 547 547
Size: 6581
DrdI GACnn_nn'nnGTC
Cuts at: 4287 5145 4287
Size: 858 5723
EaeI y'GGCC_r
Cuts at: 167 264 667 2009 4166 4340 4731 4758 6103
Size: 97 403 1342 2157 174 391 27 1345
Cuts at: 6103 167
Size: 645
Fragments arranged by size:
2157 1345 1342 645 403 391 174 97
27
EagI C'GGCC_G
Cuts at: 264 4166 264
Size: 3902 2679
EarI CTCTTCn'nnn_
Cuts at: 256 374 594 1421 2355 2660 2815 3225 3344
Size: 118 220 827 934 305 155 410 119
Cuts at: 3344 4604 4814 256
Size: 1260 210 2023
Fragments arranged by size:
2023 1260 934 827 410 305 220 210
155 119 118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3163 4789 5099 5245 6391 3163
Size: 1626 310 146 1146 3353
Fragments arranged by size:
3353 1626 1146 310 146
EcoICRI GAG'CTC
Cuts at: 815 1737 3593 815
Size: 922 1856 3803
Fragments arranged by size:
3803 1856 922
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 517 579 998 1075 1325 1568 2239 2294
Size: 92 62 419 77 250 243 671 55
Cuts at: 2294 2485 2632 2746 2845 3032 3325 4406 4838
Size: 191 147 114 99 187 293 1081 432
Cuts at: 4838 5585 5853 425
Size: 747 268 1153
Fragments arranged by size:
1153 1081 747 671 432 419 293 268
250 243 191 187 147 114 99 92
77 62 55
EcoNI CCTnn'n_nnAGG
Cuts at: 844 844
Size: 6581
EcoO109I rG'GnC_Cy
Cuts at: 149 159 3720 149
Size: 10 3561 3010
Fragments arranged by size:
3561 3010 10
EcoRI G'AATT_C
Cuts at: 3585 3585
Size: 6581
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 877 4600 877
Size: 3723 2858
FspI TGC'GCA
Cuts at: 4362 4362
Size: 6581
HaeII r_GCGC'y
Cuts at: 2769 3108 4015 4263 5285 6264 2769
Size: 339 907 248 1022 979 3086
Fragments arranged by size:
3086 1022 979 907 339 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1305 1337 1344 1376 1911 1943 1998 2009 2030
Size: 32 7 32 535 32 55 11 21
Cuts at: 2030 2041 2627 2632 2659 2664 3340 3372 4423
Size: 11 586 5 27 5 676 32 1051
Cuts at: 4423 4453 4455 4485 1305
Size: 30 2 30 3401
Fragments arranged by size:
3401 1051 676 586 535 55 32 32
32 32 30 30 27 21 11 11
7 5 5 2
HincII GTy'rAC
Cuts at: 1122 1328 2100 3620 1122
Size: 206 772 1520 4083
Fragments arranged by size:
4083 1520 772 206
HindIII A'AGCT_T
Cuts at: 960 3257 3504 960
Size: 2297 247 4037
Fragments arranged by size:
4037 2297 247
KasI G'GCGC_C
Cuts at: 4011 4259 4011
Size: 248 6333
KpnI G_GTAC'C
Cuts at: 146 3601 146
Size: 3455 3126
MfeI C'AATT_G
Cuts at: 3383 3383
Size: 6581
MluI A'CGCG_T
Cuts at: 6014 6014
Size: 6581
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2174 3251 5252 5436 2174
Size: 1077 2001 184 3319
Fragments arranged by size:
3319 2001 1077 184
MscI TGG'CCA
Cuts at: 669 2011 4342 669
Size: 1342 2331 2908
Fragments arranged by size:
2908 2331 1342
MslI CAynn'nnrTG
Cuts at: 560 4570 4697 560
Size: 4010 127 2444
Fragments arranged by size:
4010 2444 127
MspA1I CmG'CkG
Cuts at: 132 165 420 537 2520 2915 3141 3692 4006
Size: 33 255 117 1983 395 226 551 314
Cuts at: 4006 4366 5379 5624 132
Size: 360 1013 245 1089
Fragments arranged by size:
1983 1089 1013 551 395 360 314 255
245 226 117 33
NaeI GCC'GGC
Cuts at: 268 4762 268
Size: 4494 2087
NarI GG'CG_CC
Cuts at: 4012 4260 4012
Size: 248 6333
NcoI C'CATG_G
Cuts at: 2398 2398
Size: 6581
NgoMIV G'CCGG_C
Cuts at: 266 4760 266
Size: 4494 2087
NheI G'CTAG_C
Cuts at: 202 202
Size: 6581
NsiI A_TGCA'T
Cuts at: 1029 1029
Size: 6581
NspI r_CATG'y
Cuts at: 861 1027 2038 3634 4134 5041 861
Size: 166 1011 1596 500 907 2401
Fragments arranged by size:
2401 1596 1011 907 500 166
PasI CC'CwG_GG
Cuts at: 222 222
Size: 6581
PciI A'CATG_T
Cuts at: 5037 5037
Size: 6581
PflMI CCAn_nnn'nTGG
Cuts at: 2267 2267
Size: 6581
PfoI T'CCnGG_A
Cuts at: 2543 2543
Size: 6581
PmeI GTTT'AAAC
Cuts at: 3580 3580
Size: 6581
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2062 2094 5720 5752 2062
Size: 32 3626 32 2891
Fragments arranged by size:
3626 2891 32 32
PpuMI rG'GwC_Cy
Cuts at: 149 149
Size: 6581
PshAI GACnn'nnGTC
Cuts at: 1518 1518
Size: 6581
PspOMI G'GGCC_C
Cuts at: 159 178 159
Size: 19 6562
PstI C_TGCA'G
Cuts at: 234 1699 2166 2318 3628 4313 234
Size: 1465 467 152 1310 685 2502
Fragments arranged by size:
2502 1465 1310 685 467 152
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6581
PvuII CAG'CTG
Cuts at: 420 537 2520 2915 3141 4006 4366 420
Size: 117 1983 395 226 865 360 2635
Fragments arranged by size:
2635 1983 865 395 360 226 117
RsrII CG'GwC_CG
Cuts at: 4776 4776
Size: 6581
SacI G_AGCT'C
Cuts at: 817 1739 3595 817
Size: 922 1856 3803
Fragments arranged by size:
3803 1856 922
SacII CC_GC'GG
Cuts at: 133 133
Size: 6581
SalI G'TCGA_C
Cuts at: 3618 3618
Size: 6581
SanDI GG'GwC_CC
Cuts at: 149 149
Size: 6581
SapI GCTCTTCn'nnn_
Cuts at: 256 3225 3344 4604 4814 256
Size: 2969 119 1260 210 2023
Fragments arranged by size:
2969 2023 1260 210 119
SbfI CC_TGCA'GG
Cuts at: 3628 3628
Size: 6581
ScaI AGT'ACT
Cuts at: 3460 6098 3460
Size: 2638 3943
SfcI C'TryA_G
Cuts at: 33 230 1268 1643 1695 2162 2314 2626 3624
Size: 197 1038 375 52 467 152 312 998
Cuts at: 3624 4309 5302 5493 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 1038 998 993 685 467 375 312
197 191 152 52
SfoI GGC'GCC
Cuts at: 4013 4261 4013
Size: 248 6333
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6581
SmaI CCC'GGG
Cuts at: 140 3603 140
Size: 3463 3118
SmlI C'TyrA_G
Cuts at: 2065 2238 2302 2566 3024 3736 4651 5143 5405
Size: 173 64 264 458 712 915 492 262
Cuts at: 5405 2065
Size: 3241
Fragments arranged by size:
3241 915 712 492 458 264 262 173
64
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6581
SphI G_CATG'C
Cuts at: 861 1027 2038 3634 4134 861
Size: 166 1011 1596 500 3308
Fragments arranged by size:
3308 1596 1011 500 166
SspI AAT'ATT
Cuts at: 2298 3556 2298
Size: 1258 5323
StyI C'CwwG_G
Cuts at: 2398 3715 4069 2398
Size: 1317 354 4910
Fragments arranged by size:
4910 1317 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2527 4164 4297 4756 4965 2527
Size: 1637 133 459 209 4143
Fragments arranged by size:
4143 1637 459 209 133
TatI w'GTAC_w
Cuts at: 3458 6096 3458
Size: 2638 3943
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 293 325 1880 1912 6098 6130 293
Size: 32 1555 32 4186 32 744
Fragments arranged by size:
4186 1555 744 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2095 4378 2095
Size: 2283 4298
XbaI T'CTAG_A
Cuts at: 1501 3612 1501
Size: 2111 4470
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4076 4076
Size: 6581
XmaI C'CCGG_G
Cuts at: 138 3601 138
Size: 3463 3118
XmnI GAAnn'nnTTC
Cuts at: 882 2393 882
Size: 1511 5070
ZraI GAC'GTC
Cuts at: 1975 1975
Size: 6581
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AhdI AlfI AscI AvrII BbvCI BcgI BmgBI
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I CspCI
EcoRV FseI FspAI HpaI NdeI NotI NruI PacI
PmlI PsiI PspXI PsrI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI