Restriction map for
pF1KB0170
(Circular) (Six-base) MAPSORT of: pF1KB0170.seq Check: 6168 from: 1 to: 6326
pF1KB0170 6326 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3175 3175
Size: 6326
AatII G_ACGT'C
Cuts at: 448 448
Size: 6326
AccI GT'mk_AC
Cuts at: 296 2267 3364 296
Size: 1971 1097 3258
Fragments arranged by size:
3258 1971 1097
Acc65I G'GTAC_C
Cuts at: 3342 3342
Size: 6326
AclI AA'CG_TT
Cuts at: 6166 6166
Size: 6326
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1202 1813 1966 2647 3013 4151 4583 4936
Size: 280 611 153 681 366 1138 432 353
Cuts at: 4936 5598 922
Size: 662 1650
Fragments arranged by size:
1650 1138 681 662 611 432 366 353
280 153
AflII C'TTAA_G
Cuts at: 1096 3052 1096
Size: 1956 4370
AflIII A'CryG_T
Cuts at: 2078 3678 5478 5759 2078
Size: 1600 1800 281 2645
Fragments arranged by size:
2645 1800 1600 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 296 296
Size: 6326
AlwNI CAG_nnn'CTG
Cuts at: 519 3631 5069 519
Size: 3112 1438 1776
Fragments arranged by size:
3112 1776 1438
ApaLI G'TGCA_C
Cuts at: 2033 5164 2033
Size: 3131 3195
ApoI r'AATT_y
Cuts at: 310 350 709 750 1208 1435 1461 1584 1786
Size: 40 359 41 458 227 26 123 202
Cuts at: 1786 1819 1946 2243 2430 3178 3330 5679 5935
Size: 33 127 297 187 748 152 2349 256
Cuts at: 5935 310
Size: 701
Fragments arranged by size:
2349 748 701 458 359 297 256 227
202 187 152 127 123 41 40 33
26
AseI AT'TA_AT
Cuts at: 20 844 1363 20
Size: 824 519 4983
Fragments arranged by size:
4983 824 519
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6326
AvaI C'yCGr_G
Cuts at: 3346 3346
Size: 6326
BamHI G'GATC_C
Cuts at: 3351 3351
Size: 6326
BanI G'GyrC_C
Cuts at: 3342 3756 4004 4039 3342
Size: 414 248 35 5629
Fragments arranged by size:
5629 414 248 35
BanII G_rGCy'C
Cuts at: 231 1779 3340 4370 231
Size: 1548 1561 1030 2187
Fragments arranged by size:
2187 1561 1548 1030
BbeI G_GCGC'C
Cuts at: 3760 4008 3760
Size: 248 6078
BbsI GAAGACnn'nnnn_
Cuts at: 1801 3197 1801
Size: 1396 4930
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1884 1918 90
Size: 34 1760 34 4498
Fragments arranged by size:
4498 1760 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1440 4224 5280 6326 1440
Size: 2784 1056 1046 1440
Fragments arranged by size:
2784 1440 1056 1046
BclI T'GATC_A
Cuts at: 1107 1413 3846 1107
Size: 306 2433 3587
Fragments arranged by size:
3587 2433 306
BfrBI ATG'CAT
Cuts at: 1632 1827 2931 2935 1632
Size: 195 1104 4 5023
Fragments arranged by size:
5023 1104 195 4
BglII A'GATC_T
Cuts at: 3841 5878 3841
Size: 2037 4289
BlpI GC'TnA_GC
Cuts at: 3438 3438
Size: 6326
Bme1580I G_kGCm'C
Cuts at: 2037 3951 4044 5168 2037
Size: 1914 93 1124 3195
Fragments arranged by size:
3195 1914 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 3538 3619 3954 6098 3538
Size: 81 335 2144 3766
Fragments arranged by size:
3766 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4693 4693
Size: 6326
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2860 2860
Size: 6326
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 648 3068 3502 4381 5095 5393 230
Size: 418 2420 434 879 714 298 1163
Fragments arranged by size:
2420 1163 879 714 434 418 298
BsaI GGTCTCn'nnnn_
Cuts at: 829 1825 6030 829
Size: 996 4205 1125
Fragments arranged by size:
4205 1125 996
BsaAI yAC'GTr
Cuts at: 3679 3679
Size: 6326
BsaBI GATnn'nnATC
Cuts at: 6 3840 5877 6
Size: 3834 2037 455
Fragments arranged by size:
3834 2037 455
BsaHI Gr'CG_yC
Cuts at: 445 3757 4005 4707 6203 445
Size: 3312 248 702 1496 568
Fragments arranged by size:
3312 1496 702 568 248
BsaWI w'CCGG_w
Cuts at: 3515 4036 4714 5125 5272 5546 3515
Size: 521 678 411 147 274 4295
Fragments arranged by size:
4295 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1412 1442 1412
Size: 30 6296
BseYI C'CCAG_C
Cuts at: 230 2484 2688 3751 4509 5174 5582 230
Size: 2254 204 1063 758 665 408 974
Fragments arranged by size:
2254 1063 974 758 665 408 204
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1071 1071
Size: 6326
BsiEI CG_ry'CG
Cuts at: 104 3868 3914 5144 104
Size: 3764 46 1230 1286
Fragments arranged by size:
3764 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1779 2037 3340 4118 4308 5168 1779
Size: 258 1303 778 190 860 2937
Fragments arranged by size:
2937 1303 860 778 258 190
BsmI GAATG_Cn'
Cuts at: 2596 2692 2596
Size: 96 6230
Bsp1286I G_dGCh'C
Cuts at: 231 1779 2037 3340 3951 4044 4118 4308 4370
Size: 1548 258 1303 611 93 74 190 62
Cuts at: 4370 5168 231
Size: 798 1389
Fragments arranged by size:
1548 1389 1303 798 611 258 190 93
74 62
BspHI T'CATG_A
Cuts at: 1439 4758 1439
Size: 3319 3007
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3175 3376 3590 3892 4273 3175
Size: 201 214 302 381 5228
Fragments arranged by size:
5228 381 302 214 201
BsrBI CCG'CTC
Cuts at: 50 4618 4672 6156 6321 50
Size: 4568 54 1484 165 55
Fragments arranged by size:
4568 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 711 790 1634 4238 711
Size: 79 844 2604 2799
Fragments arranged by size:
2799 2604 844 79
BsrFI r'CCGG_y
Cuts at: 4324 4505 4324
Size: 181 6145
BsrGI T'GTAC_A
Cuts at: 771 1742 771
Size: 971 5355
BssSI C'ACGA_G
Cuts at: 4597 5305 4597
Size: 708 5618
BstAPI GCAn_nnn'nTGC
Cuts at: 139 2698 3703 139
Size: 2559 1005 2762
Fragments arranged by size:
2762 2559 1005
BstBI TT'CG_AA
Cuts at: 888 1817 4687 888
Size: 929 2870 2527
Fragments arranged by size:
2870 2527 929
BstXI CCAn_nnnn'nTGG
Cuts at: 2133 2311 2133
Size: 178 6148
BstYI r'GATC_y
Cuts at: 1 1674 2148 3351 3818 3841 4176 4422 4728
Size: 1673 474 1203 467 23 335 246 306
Cuts at: 4728 4740 4826 5878 1
Size: 12 86 1052 449
Fragments arranged by size:
1673 1203 1052 474 467 449 335 306
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 2268 2268
Size: 6326
BtgI C'CryG_G
Cuts at: 216 216
Size: 6326
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4276 4457 4620 4276
Size: 181 163 5982
Fragments arranged by size:
5982 181 163
BtsI GCAGTG_nn'
Cuts at: 3569 3569
Size: 6326
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1751 1786 1751
Size: 35 6291
DraI TTT'AAA
Cuts at: 266 689 1007 1206 2375 3325 266
Size: 423 318 199 1169 950 3267
Fragments arranged by size:
3267 1169 950 423 318 199
DrdI GACnn_nn'nnGTC
Cuts at: 4032 5376 4032
Size: 1344 4982
EaeI y'GGCC_r
Cuts at: 544 2519 3911 4085 4476 4503 5848 544
Size: 1975 1392 174 391 27 1345 1022
Fragments arranged by size:
1975 1392 1345 1022 391 174 27
EagI C'GGCC_G
Cuts at: 3911 3911
Size: 6326
EarI CTCTTCn'nnn_
Cuts at: 1631 3209 3242 4349 4559 1631
Size: 1578 33 1107 210 3398
Fragments arranged by size:
3398 1578 1107 210 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4534 5276 5422 6136 4534
Size: 742 146 714 4724
Fragments arranged by size:
4724 742 714 146
EcoICRI GAG'CTC
Cuts at: 1777 3338 1777
Size: 1561 4765
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1202 1813 1966 2647 2860 3013 4151 4583
Size: 280 611 153 681 213 153 1138 432
Cuts at: 4583 4936 5598 922
Size: 353 662 1650
Fragments arranged by size:
1650 1138 681 662 611 432 353 280
213 153 153
EcoNI CCTnn'n_nnAGG
Cuts at: 3235 3235
Size: 6326
EcoO109I rG'GnC_Cy
Cuts at: 1155 3465 1155
Size: 2310 4016
EcoRI G'AATT_C
Cuts at: 709 1435 1819 3330 709
Size: 726 384 1511 3705
Fragments arranged by size:
3705 1511 726 384
EcoRV GAT'ATC
Cuts at: 180 180
Size: 6326
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2387 3078 4345 2387
Size: 691 1267 4368
Fragments arranged by size:
4368 1267 691
FspI TGC'GCA
Cuts at: 4107 4107
Size: 6326
HaeII r_GCGC'y
Cuts at: 3760 4008 5238 6009 3760
Size: 248 1230 771 4077
Fragments arranged by size:
4077 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1118 1150 2835 2867 4168 4198 4200 4230 1118
Size: 32 1685 32 1301 30 2 30 3214
Fragments arranged by size:
3214 1685 1301 32 32 30 30 2
HincII GTy'rAC
Cuts at: 297 1300 1806 2532 3012 3365 297
Size: 1003 506 726 480 353 3258
Fragments arranged by size:
3258 1003 726 506 480 353
HindIII A'AGCT_T
Cuts at: 1543 1543
Size: 6326
HpaI GTT'AAC
Cuts at: 1806 3012 1806
Size: 1206 5120
KasI G'GCGC_C
Cuts at: 3756 4004 3756
Size: 248 6078
KpnI G_GTAC'C
Cuts at: 3346 3346
Size: 6326
MluI A'CGCG_T
Cuts at: 5759 5759
Size: 6326
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 549 1899 2193 2429 5085 5269 549
Size: 1350 294 236 2656 184 1606
Fragments arranged by size:
2656 1606 1350 294 236 184
MscI TGG'CCA
Cuts at: 546 2521 4087 546
Size: 1975 1566 2785
Fragments arranged by size:
2785 1975 1566
MslI CAynn'nnrTG
Cuts at: 278 1307 4315 4442 278
Size: 1029 3008 127 2162
Fragments arranged by size:
3008 2162 1029 127
MspA1I CmG'CkG
Cuts at: 153 2488 2557 3437 3751 4111 4895 5140 153
Size: 2335 69 880 314 360 784 245 1339
Fragments arranged by size:
2335 1339 880 784 360 314 245 69
NaeI GCC'GGC
Cuts at: 4507 4507
Size: 6326
NarI GG'CG_CC
Cuts at: 3757 4005 3757
Size: 248 6078
NcoI C'CATG_G
Cuts at: 216 216
Size: 6326
NgoMIV G'CCGG_C
Cuts at: 4505 4505
Size: 6326
NsiI A_TGCA'T
Cuts at: 1634 1829 2933 2937 1634
Size: 195 1104 4 5023
Fragments arranged by size:
5023 1104 195 4
NspI r_CATG'y
Cuts at: 702 778 2082 2935 3379 3879 5482 702
Size: 76 1304 853 444 500 1603 1546
Fragments arranged by size:
1603 1546 1304 853 500 444 76
PasI CC'CwG_GG
Cuts at: 819 819
Size: 6326
PciI A'CATG_T
Cuts at: 2078 5478 2078
Size: 3400 2926
PflMI CCAn_nnn'nTGG
Cuts at: 174 542 1854 2744 174
Size: 368 1312 890 3756
Fragments arranged by size:
3756 1312 890 368
PfoI T'CCnGG_A
Cuts at: 1847 2997 1847
Size: 1150 5176
PmeI GTTT'AAAC
Cuts at: 3325 3325
Size: 6326
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4772 4804 4772
Size: 32 6294
PshAI GACnn'nnGTC
Cuts at: 292 292
Size: 6326
PsiI TTA'TAA
Cuts at: 863 1669 863
Size: 806 5520
PstI C_TGCA'G
Cuts at: 2298 2838 3373 4058 2298
Size: 540 535 685 4566
Fragments arranged by size:
4566 685 540 535
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6326
PvuII CAG'CTG
Cuts at: 153 2488 2557 3751 4111 153
Size: 2335 69 1194 360 2368
Fragments arranged by size:
2368 2335 1194 360 69
RsrII CG'GwC_CG
Cuts at: 4521 4521
Size: 6326
SacI G_AGCT'C
Cuts at: 1779 3340 1779
Size: 1561 4765
SalI G'TCGA_C
Cuts at: 295 3363 295
Size: 3068 3258
SapI GCTCTTCn'nnn_
Cuts at: 4349 4559 4349
Size: 210 6116
SbfI CC_TGCA'GG
Cuts at: 3373 3373
Size: 6326
ScaI AGT'ACT
Cuts at: 254 2514 5843 254
Size: 2260 3329 737
Fragments arranged by size:
3329 2260 737
SfcI C'TryA_G
Cuts at: 33 1248 1799 2294 2834 3369 4054 5022 5213
Size: 1215 551 495 540 535 685 968 191
Cuts at: 5213 33
Size: 1146
Fragments arranged by size:
1215 1146 968 685 551 540 535 495
191
SfoI GGC'GCC
Cuts at: 3758 4006 3758
Size: 248 6078
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6326
SmaI CCC'GGG
Cuts at: 3348 3348
Size: 6326
SmlI C'TyrA_G
Cuts at: 245 627 1096 3052 3083 3481 4396 5110 5372
Size: 382 469 1956 31 398 915 714 262
Cuts at: 5372 245
Size: 1199
Fragments arranged by size:
1956 1199 915 714 469 398 382 262
31
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6326
SphI G_CATG'C
Cuts at: 2935 3379 3879 2935
Size: 444 500 5382
Fragments arranged by size:
5382 500 444
SspI AAT'ATT
Cuts at: 1017 2289 2573 1017
Size: 1272 284 4770
Fragments arranged by size:
4770 1272 284
StyI C'CwwG_G
Cuts at: 216 391 782 3460 3814 216
Size: 175 391 2678 354 2728
Fragments arranged by size:
2728 2678 391 354 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2382 3909 4042 4501 4710 2382
Size: 1527 133 459 209 3998
Fragments arranged by size:
3998 1527 459 209 133
TatI w'GTAC_w
Cuts at: 252 771 1742 2052 2075 2512 2983 5841 252
Size: 519 971 310 23 437 471 2858 737
Fragments arranged by size:
2858 971 737 519 471 437 310 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1412 1444 5843 5875 1412
Size: 32 4399 32 1863
Fragments arranged by size:
4399 1863 32 32
Tth111I GACn'n_nGTC
Cuts at: 4123 4123
Size: 6326
XbaI T'CTAG_A
Cuts at: 3357 3357
Size: 6326
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3821 3821
Size: 6326
XmaI C'CCGG_G
Cuts at: 3346 3346
Size: 6326
XmnI GAAnn'nnTTC
Cuts at: 2650 2650
Size: 6326
ZraI GAC'GTC
Cuts at: 446 446
Size: 6326
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AgeI AhdI AleI AloI ApaI AscI AvrII
BaeI BbvCI BglI BmgBI BmtI Bpu10I BseRI BsiWI
BsmBI BspEI BssHII BstEII Bsu36I ClaI DraIII FseI
FspAI MfeI NdeI NheI NotI NruI PacI PmlI
PpuMI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI