Restriction map for
pF1KB0174
(Circular) (Six-base) MAPSORT of: pF1KB0174.seq Check: 133 from: 1 to: 7187
pF1KB0174 7187 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AatII G_ACGT'C
Cuts at: 1818 1818
Size: 7187
AccI GT'mk_AC
Cuts at: 360 776 2180 4225 360
Size: 416 1404 2045 3322
Fragments arranged by size:
3322 2045 1404 416
Acc65I G'GTAC_C
Cuts at: 3595 4203 3595
Size: 608 6579
AclI AA'CG_TT
Cuts at: 708 1008 1111 7027 708
Size: 300 103 5916 868
Fragments arranged by size:
5916 868 300 103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 1433 2440 2923 4022 5012 5444 6191 6459
Size: 370 1007 483 1099 990 432 747 268
Cuts at: 6459 1063
Size: 1791
Fragments arranged by size:
1791 1099 1007 990 747 483 432 370
268
AfeI AGC'GCT
Cuts at: 484 484
Size: 7187
AflIII A'CryG_T
Cuts at: 2649 3136 4539 5643 6620 2649
Size: 487 1403 1104 977 3216
Fragments arranged by size:
3216 1403 1104 977 487
AgeI A'CCGG_T
Cuts at: 2871 5575 2871
Size: 2704 4483
AhdI GACnn_n'nnGTC
Cuts at: 3486 3486
Size: 7187
AleI CACnn'nnGTG
Cuts at: 1797 1797
Size: 7187
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2990 3104 2990
Size: 114 7073
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3604 3636 3604
Size: 32 7155
AlwNI CAG_nnn'CTG
Cuts at: 504 2418 4492 6059 504
Size: 1914 2074 1567 1632
Fragments arranged by size:
2074 1914 1632 1567
ApaI G_GGCC'C
Cuts at: 4069 4069
Size: 7187
ApaLI G'TGCA_C
Cuts at: 3791 5957 3791
Size: 2166 5021
ApoI r'AATT_y
Cuts at: 1993 2071 3371 4016 4191 6540 6796 1993
Size: 78 1300 645 175 2349 256 2384
Fragments arranged by size:
2384 2349 1300 645 256 175 78
AseI AT'TA_AT
Cuts at: 20 20
Size: 7187
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7187
AvaI C'yCGr_G
Cuts at: 230 4207 230
Size: 3977 3210
AvrII C'CTAG_G
Cuts at: 1399 1399
Size: 7187
BamHI G'GATC_C
Cuts at: 282 2397 4212 282
Size: 2115 1815 3257
Fragments arranged by size:
3257 2115 1815
BanI G'GyrC_C
Cuts at: 2128 3595 4203 4617 4865 4900 2128
Size: 1467 608 414 248 35 4415
Fragments arranged by size:
4415 1467 608 414 248 35
BanII G_rGCy'C
Cuts at: 1697 3663 4069 4201 5231 1697
Size: 1966 406 132 1030 3653
Fragments arranged by size:
3653 1966 1030 406 132
BbeI G_GCGC'C
Cuts at: 4621 4869 4621
Size: 248 6939
BbsI GAAGACnn'nnnn_
Cuts at: 644 2465 3196 3482 644
Size: 1821 731 286 4349
Fragments arranged by size:
4349 1821 731 286
BbvCI CC'TCA_GC
Cuts at: 1988 1988
Size: 7187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2773 2807 2773
Size: 34 7153
BciVI GTATCCnnnnn_n'
Cuts at: 2111 5085 5846 7187 2111
Size: 2974 761 1341 2111
Fragments arranged by size:
2974 2111 1341 761
BclI T'GATC_A
Cuts at: 2745 4707 2745
Size: 1962 5225
BfrBI ATG'CAT
Cuts at: 3411 3411
Size: 7187
BglII A'GATC_T
Cuts at: 3205 4702 6739 3205
Size: 1497 2037 3653
Fragments arranged by size:
3653 2037 1497
BlpI GC'TnA_GC
Cuts at: 854 2990 4299 854
Size: 2136 1309 3742
Fragments arranged by size:
3742 2136 1309
Bme1580I G_kGCm'C
Cuts at: 3795 4069 4812 4905 5961 3795
Size: 274 743 93 1056 5021
Fragments arranged by size:
5021 1056 743 274 93
BmrI ACTGGGnnnn_n'
Cuts at: 1089 2597 2774 4399 4480 4815 6959 1089
Size: 1508 177 1625 81 335 2144 1317
Fragments arranged by size:
2144 1625 1508 1317 335 177 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 451 5554 451
Size: 5103 2084
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 554 3446 340
Size: 214 2892 4081
Fragments arranged by size:
4081 2892 214
Bpu10I CC'TnA_GC
Cuts at: 1988 1988
Size: 7187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1440 1939 4363 5242 5734 6032 1440
Size: 499 2424 879 492 298 2595
Fragments arranged by size:
2595 2424 879 499 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6891 6891
Size: 7187
BsaAI yAC'GTr
Cuts at: 1888 4540 1888
Size: 2652 4535
BsaBI GATnn'nnATC
Cuts at: 6 1880 3410 3863 4701 6738 6
Size: 1874 1530 453 838 2037 455
Fragments arranged by size:
2037 1874 1530 838 455 453
BsaHI Gr'CG_yC
Cuts at: 1815 3045 3672 4618 4866 5568 7064 1815
Size: 1230 627 946 248 702 1496 1938
Fragments arranged by size:
1938 1496 1230 946 702 627 248
BsaWI w'CCGG_w
Cuts at: 1485 2871 4376 4897 5575 5849 5996 6407 1485
Size: 1386 1505 521 678 274 147 411 2265
Fragments arranged by size:
2265 1505 1386 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3224 3254 3224
Size: 30 7157
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 696 818 1935 2765 3812 696
Size: 122 1117 830 1047 4071
Fragments arranged by size:
4071 1117 1047 830 122
BseYI C'CCAG_C
Cuts at: 3476 4612 5370 5947 6443 3476
Size: 1136 758 577 496 4220
Fragments arranged by size:
4220 1136 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 332
Size: 7187
BsiEI CG_ry'CG
Cuts at: 104 4729 4775 5983 104
Size: 4625 46 1208 1308
Fragments arranged by size:
4625 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1697 3432 3795 4201 4979 5169 5961 1697
Size: 1735 363 406 778 190 792 2923
Fragments arranged by size:
2923 1735 792 778 406 363 190
Bsp1286I G_dGCh'C
Cuts at: 1697 3432 3663 3795 4069 4201 4812 4905 4979
Size: 1735 231 132 274 132 611 93 74
Cuts at: 4979 5169 5231 5961 1697
Size: 190 62 730 2923
Fragments arranged by size:
2923 1735 730 611 274 231 190 132
132 93 74 62
BspEI T'CCGG_A
Cuts at: 1485 6407 1485
Size: 4922 2265
BspHI T'CATG_A
Cuts at: 177 2486 2538 3075 4092 6363 177
Size: 2309 52 537 1017 2271 1001
Fragments arranged by size:
2309 2271 1017 1001 537 52
BspMI ACCTGCnnnn'nnnn_
Cuts at: 829 4237 4451 4753 5134 829
Size: 3408 214 302 381 2882
Fragments arranged by size:
3408 2882 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5479 5533 7017 7182 50
Size: 5429 54 1484 165 55
Fragments arranged by size:
5429 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 128 289 827 1053 1392 2979 3504 5099 128
Size: 161 538 226 339 1587 525 1595 2216
Fragments arranged by size:
2216 1595 1587 538 525 339 226 161
BsrFI r'CCGG_y
Cuts at: 2871 5185 5366 5575 2871
Size: 2314 181 209 4483
Fragments arranged by size:
4483 2314 209 181
BssSI C'ACGA_G
Cuts at: 5458 5816 5458
Size: 358 6829
BstAPI GCAn_nnn'nTGC
Cuts at: 423 4564 423
Size: 4141 3046
BstBI TT'CG_AA
Cuts at: 2913 5548 2913
Size: 2635 4552
BstEII G'GTnAC_C
Cuts at: 573 573
Size: 7187
BstXI CCAn_nnnn'nTGG
Cuts at: 2520 3041 3313 2520
Size: 521 272 6394
Fragments arranged by size:
6394 521 272
BstYI r'GATC_y
Cuts at: 1 143 282 500 756 2397 3205 4212 4679
Size: 142 139 218 256 1641 808 1007 467
Cuts at: 4679 4702 5037 5283 6295 6381 6393 6739 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1641 1012 1007 808 467 449 346 335
256 246 218 142 139 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1217 3484 1217
Size: 2267 4920
BtgI C'CryG_G
Cuts at: 107 2132 3599 3834 4082 107
Size: 2025 1467 235 248 3212
Fragments arranged by size:
3212 2025 1467 248 235
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5137 5318 5481 5137
Size: 181 163 6843
Fragments arranged by size:
6843 181 163
BtsI GCAGTG_nn'
Cuts at: 2974 3597 3795 4430 2974
Size: 623 198 635 5731
Fragments arranged by size:
5731 635 623 198
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1161 1196 1161
Size: 35 7152
DraI TTT'AAA
Cuts at: 222 2267 3512 3524 4186 222
Size: 2045 1245 12 662 3223
Fragments arranged by size:
3223 2045 1245 662 12
DrdI GACnn_nn'nnGTC
Cuts at: 4893 5751 4893
Size: 858 6329
EaeI y'GGCC_r
Cuts at: 4772 4946 5337 5364 6709 4772
Size: 174 391 27 1345 5250
Fragments arranged by size:
5250 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4772 4772
Size: 7187
EarI CTCTTCn'nnn_
Cuts at: 808 2378 2447 5210 5420 808
Size: 1570 69 2763 210 2575
Fragments arranged by size:
2763 2575 1570 210 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2378 5395 5705 5851 6997 2378
Size: 3017 310 146 1146 2568
Fragments arranged by size:
3017 2568 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1695 4199 1695
Size: 2504 4683
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 554 1063 1433 2440 2923 3446 4022 5012
Size: 214 509 370 1007 483 523 576 990
Cuts at: 5012 5444 6191 6459 340
Size: 432 747 268 1068
Fragments arranged by size:
1068 1007 990 747 576 523 509 483
432 370 268 214
EcoNI CCTnn'n_nnAGG
Cuts at: 3978 3978
Size: 7187
EcoO109I rG'GnC_Cy
Cuts at: 2727 3480 3932 4065 4326 2727
Size: 753 452 133 261 5588
Fragments arranged by size:
5588 753 452 261 133
EcoRI G'AATT_C
Cuts at: 3371 4191 3371
Size: 820 6367
EcoRV GAT'ATC
Cuts at: 1473 1473
Size: 7187
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2482 2620 5206 2482
Size: 138 2586 4463
Fragments arranged by size:
4463 2586 138
FspI TGC'GCA
Cuts at: 4968 4968
Size: 7187
HaeII r_GCGC'y
Cuts at: 486 4621 4869 5891 6870 486
Size: 4135 248 1022 979 803
Fragments arranged by size:
4135 1022 979 803 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2184 2216 3070 3102 3106 3138 3224 3256 3472
Size: 32 854 32 4 32 86 32 216
Cuts at: 3472 3504 3511 3543 3761 3793 5029 5059 5061
Size: 32 7 32 218 32 1236 30 2
Cuts at: 5061 5091 2184
Size: 30 4280
Fragments arranged by size:
4280 1236 854 218 216 86 32 32
32 32 32 32 32 30 30 7
4 2
HincII GTy'rAC
Cuts at: 1430 2816 4226 1430
Size: 1386 1410 4391
Fragments arranged by size:
4391 1410 1386
KasI G'GCGC_C
Cuts at: 4617 4865 4617
Size: 248 6939
KpnI G_GTAC'C
Cuts at: 3599 4207 3599
Size: 608 6579
MluI A'CGCG_T
Cuts at: 6620 6620
Size: 7187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 562 5858 6042 562
Size: 5296 184 1707
Fragments arranged by size:
5296 1707 184
MscI TGG'CCA
Cuts at: 4948 4948
Size: 7187
MslI CAynn'nnrTG
Cuts at: 1673 1797 1886 2537 3039 3074 5176 5303 1673
Size: 124 89 651 502 35 2102 127 3557
Fragments arranged by size:
3557 2102 651 502 127 124 89 35
MspA1I CmG'CkG
Cuts at: 511 2173 3715 3883 4298 4612 4972 5985 6230
Size: 1662 1542 168 415 314 360 1013 245
Cuts at: 6230 511
Size: 1468
Fragments arranged by size:
1662 1542 1468 1013 415 360 314 245
168
NaeI GCC'GGC
Cuts at: 5368 5368
Size: 7187
NarI GG'CG_CC
Cuts at: 4618 4866 4618
Size: 248 6939
NcoI C'CATG_G
Cuts at: 107 3599 3834 107
Size: 3492 235 3460
Fragments arranged by size:
3492 3460 235
NgoMIV G'CCGG_C
Cuts at: 5366 5366
Size: 7187
NsiI A_TGCA'T
Cuts at: 3413 3413
Size: 7187
NspI r_CATG'y
Cuts at: 2349 2653 2694 2721 3140 4240 4740 5647 2349
Size: 304 41 27 419 1100 500 907 3889
Fragments arranged by size:
3889 1100 907 500 419 304 41 27
PciI A'CATG_T
Cuts at: 2649 3136 5643 2649
Size: 487 2507 4193
Fragments arranged by size:
4193 2507 487
PflMI CCAn_nnn'nTGG
Cuts at: 2114 3037 2114
Size: 923 6264
PfoI T'CCnGG_A
Cuts at: 1595 4010 1595
Size: 2415 4772
PmeI GTTT'AAAC
Cuts at: 4186 4186
Size: 7187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6326 6358 6326
Size: 32 7155
PpuMI rG'GwC_Cy
Cuts at: 3480 3932 3480
Size: 452 6735
PshAI GACnn'nnGTC
Cuts at: 2845 2845
Size: 7187
PsiI TTA'TAA
Cuts at: 1252 1252
Size: 7187
PspOMI G'GGCC_C
Cuts at: 4065 4065
Size: 7187
PspXI vC'TCGA_Gb
Cuts at: 230 230
Size: 7187
PstI C_TGCA'G
Cuts at: 1125 3858 4234 4919 1125
Size: 2733 376 685 3393
Fragments arranged by size:
3393 2733 685 376
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7187
PvuII CAG'CTG
Cuts at: 511 2173 3715 3883 4612 4972 511
Size: 1662 1542 168 729 360 2726
Fragments arranged by size:
2726 1662 1542 729 360 168
RsrII CG'GwC_CG
Cuts at: 5382 5382
Size: 7187
SacI G_AGCT'C
Cuts at: 1697 4201 1697
Size: 2504 4683
SalI G'TCGA_C
Cuts at: 4224 4224
Size: 7187
SapI GCTCTTCn'nnn_
Cuts at: 5210 5420 5210
Size: 210 6977
SbfI CC_TGCA'GG
Cuts at: 4234 4234
Size: 7187
ScaI AGT'ACT
Cuts at: 210 3954 6704 210
Size: 3744 2750 693
Fragments arranged by size:
3744 2750 693
SfcI C'TryA_G
Cuts at: 33 152 1121 1264 3846 3854 3978 4230 4915
Size: 119 969 143 2582 8 124 252 685
Cuts at: 4915 5908 6099 33
Size: 993 191 1121
Fragments arranged by size:
2582 1121 993 969 685 252 191 143
124 119 8
SfoI GGC'GCC
Cuts at: 4619 4867 4619
Size: 248 6939
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7187
SmaI CCC'GGG
Cuts at: 4209 4209
Size: 7187
SmlI C'TyrA_G
Cuts at: 230 1419 1954 4342 5257 5749 6011 230
Size: 1189 535 2388 915 492 262 1406
Fragments arranged by size:
2388 1406 1189 915 535 492 262
SpeI A'CTAG_T
Cuts at: 67 3955 67
Size: 3888 3299
SphI G_CATG'C
Cuts at: 4240 4740 4240
Size: 500 6687
SspI AAT'ATT
Cuts at: 1064 1479 1758 1064
Size: 415 279 6493
Fragments arranged by size:
6493 415 279
StuI AGG'CCT
Cuts at: 251 251
Size: 7187
StyI C'CwwG_G
Cuts at: 107 1103 1399 3261 3599 3834 4321 4675 107
Size: 996 296 1862 338 235 487 354 2619
Fragments arranged by size:
2619 1862 996 487 354 338 296 235
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3693 4770 4903 5362 5571 3693
Size: 1077 133 459 209 5309
Fragments arranged by size:
5309 1077 459 209 133
TatI w'GTAC_w
Cuts at: 208 1605 2167 2884 3952 6702 208
Size: 1397 562 717 1068 2750 693
Fragments arranged by size:
2750 1397 1068 717 693 562
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 615 647 6704 6736 615
Size: 32 6057 32 1066
Fragments arranged by size:
6057 1066 32 32
Tth111I GACn'n_nGTC
Cuts at: 4984 4984
Size: 7187
XbaI T'CTAG_A
Cuts at: 3375 4153 4218 3375
Size: 778 65 6344
Fragments arranged by size:
6344 778 65
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3038 3747 4682 3038
Size: 709 935 5543
Fragments arranged by size:
5543 935 709
XhoI C'TCGA_G
Cuts at: 230 230
Size: 7187
XmaI C'CCGG_G
Cuts at: 4207 4207
Size: 7187
XmnI GAAnn'nnTTC
Cuts at: 1112 1310 1493 2367 2482 1112
Size: 198 183 874 115 5817
Fragments arranged by size:
5817 874 198 183 115
ZraI GAC'GTC
Cuts at: 1816 1816
Size: 7187
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AscI BaeI BglI BmgBI BmtI BsiWI
BsmI BsmBI BsrGI BssHII BstZ17I ClaI DraIII FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PasI PmlI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI