Restriction map for
pF1KB0179
(Circular) (Six-base) MAPSORT of: pF1KB0179.seq Check: 1288 from: 1 to: 6773
pF1KB0179 6773 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3489 3489
Size: 6773
AccI GT'mk_AC
Cuts at: 3811 3811
Size: 6773
Acc65I G'GTAC_C
Cuts at: 3789 3789
Size: 6773
AclI AA'CG_TT
Cuts at: 6613 6613
Size: 6773
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 1265 1837 3242 4598 5030 5777 6045 472
Size: 793 572 1405 1356 432 747 268 1200
Fragments arranged by size:
1405 1356 1200 793 747 572 432 268
AflIII A'CryG_T
Cuts at: 4125 5229 6206 4125
Size: 1104 977 4692
Fragments arranged by size:
4692 1104 977
AgeI A'CCGG_T
Cuts at: 5161 5161
Size: 6773
AleI CACnn'nnGTG
Cuts at: 2191 2854 2191
Size: 663 6110
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 614 614
Size: 6773
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2501 2533 2501
Size: 32 6741
AlwNI CAG_nnn'CTG
Cuts at: 1857 1882 1911 4078 5645 1857
Size: 25 29 2167 1567 2985
Fragments arranged by size:
2985 2167 1567 29 25
ApaI G_GGCC'C
Cuts at: 3006 3333 3006
Size: 327 6446
ApaLI G'TGCA_C
Cuts at: 991 5543 991
Size: 4552 2221
ApoI r'AATT_y
Cuts at: 259 334 1351 3208 3777 6126 6382 259
Size: 75 1017 1857 569 2349 256 650
Fragments arranged by size:
2349 1857 1017 650 569 256 75
AseI AT'TA_AT
Cuts at: 20 20
Size: 6773
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6773
AvaI C'yCGr_G
Cuts at: 181 743 3749 3793 181
Size: 562 3006 44 3161
Fragments arranged by size:
3161 3006 562 44
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 169 202 3116 3149 169
Size: 33 2914 33 3793
Fragments arranged by size:
3793 2914 33 33
BamHI G'GATC_C
Cuts at: 3798 3798
Size: 6773
BanI G'GyrC_C
Cuts at: 3789 4203 4451 4486 3789
Size: 414 248 35 6076
Fragments arranged by size:
6076 414 248 35
BanII G_rGCy'C
Cuts at: 893 1007 1489 3006 3333 3787 4817 893
Size: 114 482 1517 327 454 1030 2849
Fragments arranged by size:
2849 1517 1030 482 454 327 114
BbeI G_GCGC'C
Cuts at: 4207 4455 4207
Size: 248 6525
BbsI GAAGACnn'nnnn_
Cuts at: 2433 3150 2433
Size: 717 6056
BbvCI CC'TCA_GC
Cuts at: 2256 2256
Size: 6773
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1961 1995 2621 2655 1961
Size: 34 626 34 6079
Fragments arranged by size:
6079 626 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3144 4671 5432 6773 3144
Size: 1527 761 1341 3144
Fragments arranged by size:
3144 1527 1341 761
BclI T'GATC_A
Cuts at: 266 2969 4293 266
Size: 2703 1324 2746
Fragments arranged by size:
2746 2703 1324
BglII A'GATC_T
Cuts at: 1140 4288 6325 1140
Size: 3148 2037 1588
Fragments arranged by size:
3148 2037 1588
BlpI GC'TnA_GC
Cuts at: 3673 3885 3673
Size: 212 6561
Bme1580I G_kGCm'C
Cuts at: 995 1463 3006 3333 3528 4398 4491 5547 995
Size: 468 1543 327 195 870 93 1056 2221
Fragments arranged by size:
2221 1543 1056 870 468 327 195 93
BmrI ACTGGGnnnn_n'
Cuts at: 460 1097 1641 1899 2533 2552 3007 3985 4066
Size: 637 544 258 634 19 455 978 81
Cuts at: 4066 4401 6545 460
Size: 335 2144 688
Fragments arranged by size:
2144 978 688 637 634 544 455 335
258 81 19
BmtI G_CTAG'C
Cuts at: 3247 3247
Size: 6773
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1209 1275 3764 5140 1209
Size: 66 2489 1376 2842
Fragments arranged by size:
2842 2489 1376 66
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 374 866 1154 3082 3759 374
Size: 492 288 1928 677 3388
Fragments arranged by size:
3388 1928 677 492 288
Bpu10I CC'TnA_GC
Cuts at: 1105 2019 2256 2753 1105
Size: 914 237 497 5125
Fragments arranged by size:
5125 914 497 237
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1438 1804 2887 3949 4828 5320 5618 1438
Size: 366 1083 1062 879 492 298 2593
Fragments arranged by size:
2593 1083 1062 879 492 366 298
BsaI GGTCTCn'nnnn_
Cuts at: 689 1171 1183 3660 6477 689
Size: 482 12 2477 2817 985
Fragments arranged by size:
2817 2477 985 482 12
BsaAI yAC'GTr
Cuts at: 4126 4126
Size: 6773
BsaBI GATnn'nnATC
Cuts at: 6 1121 1777 4287 6324 6
Size: 1115 656 2510 2037 455
Fragments arranged by size:
2510 2037 1115 656 455
BsaHI Gr'CG_yC
Cuts at: 2737 4204 4452 5154 6650 2737
Size: 1467 248 702 1496 2860
Fragments arranged by size:
2860 1496 1467 702 248
BsaWI w'CCGG_w
Cuts at: 480 809 3479 3962 4483 5161 5435 5582 5993
Size: 329 2670 483 521 678 274 147 411
Cuts at: 5993 480
Size: 1260
Fragments arranged by size:
2670 1260 678 521 483 411 329 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 774 780 804 810 2974 3004 3262 3292 774
Size: 6 24 6 2164 30 258 30 4255
Fragments arranged by size:
4255 2164 258 30 30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 869 1201 2017 869
Size: 332 816 5625
Fragments arranged by size:
5625 816 332
BseYI C'CCAG_C
Cuts at: 568 1583 1955 2586 2779 3639 4198 4956 5533
Size: 1015 372 631 193 860 559 758 577
Cuts at: 5533 6029 568
Size: 496 1312
Fragments arranged by size:
1312 1015 860 758 631 577 559 496
372 193
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 350 973 2043 3169 3436 350
Size: 623 1070 1126 267 3687
Fragments arranged by size:
3687 1126 1070 623 267
BsiEI CG_ry'CG
Cuts at: 104 4315 4361 5569 104
Size: 4211 46 1208 1308
Fragments arranged by size:
4211 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 995 1007 1242 2198 3575 3787 4565 4755 5547
Size: 12 235 956 1377 212 778 190 792
Cuts at: 5547 995
Size: 2221
Fragments arranged by size:
2221 1377 956 792 778 235 212 190
12
BsmI GAATG_Cn'
Cuts at: 1379 1379
Size: 6773
BsmBI CGTCTCn'nnnn_
Cuts at: 373 373
Size: 6773
Bsp1286I G_dGCh'C
Cuts at: 893 995 1007 1242 1463 1489 2198 3006 3333
Size: 102 12 235 221 26 709 808 327
Cuts at: 3333 3528 3575 3787 4398 4491 4565 4755 4817
Size: 195 47 212 611 93 74 190 62
Cuts at: 4817 5547 893
Size: 730 2119
Fragments arranged by size:
2119 808 730 709 611 327 235 221
212 195 190 102 93 74 62 47
26 12
BspEI T'CCGG_A
Cuts at: 5993 5993
Size: 6773
BspHI T'CATG_A
Cuts at: 5949 5949
Size: 6773
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3489 3823 4037 4339 4720 3489
Size: 334 214 302 381 5542
Fragments arranged by size:
5542 381 334 302 214
BsrBI CCG'CTC
Cuts at: 50 722 837 5065 5119 6603 6768 50
Size: 672 115 4228 54 1484 165 55
Fragments arranged by size:
4228 1484 672 165 115 55 54
BsrDI GCAATG_nn'
Cuts at: 1541 4685 1541
Size: 3144 3629
BsrFI r'CCGG_y
Cuts at: 4771 4952 5161 4771
Size: 181 209 6383
Fragments arranged by size:
6383 209 181
BsrGI T'GTAC_A
Cuts at: 2348 2813 2990 3251 3710 2348
Size: 465 177 261 459 5411
Fragments arranged by size:
5411 465 459 261 177
BssSI C'ACGA_G
Cuts at: 2728 5044 5402 2728
Size: 2316 358 4099
Fragments arranged by size:
4099 2316 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4150 4150
Size: 6773
BstBI TT'CG_AA
Cuts at: 5134 5134
Size: 6773
BstEII G'GTnAC_C
Cuts at: 3475 3475
Size: 6773
BstXI CCAn_nnnn'nTGG
Cuts at: 114 1304 1968 114
Size: 1190 664 4919
Fragments arranged by size:
4919 1190 664
BstYI r'GATC_y
Cuts at: 1 414 1140 1853 1879 2480 3798 4265 4288
Size: 413 726 713 26 601 1318 467 23
Cuts at: 4288 4623 4869 5881 5967 5979 6325 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1318 1012 726 713 601 467 449 413
346 335 246 86 26 23 12
Bsu36I CC'TnA_GG
Cuts at: 1388 1388
Size: 6773
BtgI C'CryG_G
Cuts at: 107 125 161 1613 2909 2978 3286 107
Size: 18 36 1452 1296 69 308 3594
Fragments arranged by size:
3594 1452 1296 308 69 36 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2624 4723 4904 5067 2624
Size: 2099 181 163 4330
Fragments arranged by size:
4330 2099 181 163
BtsI GCAGTG_nn'
Cuts at: 2279 3117 3438 4016 2279
Size: 838 321 578 5036
Fragments arranged by size:
5036 838 578 321
DraI TTT'AAA
Cuts at: 3772 3772
Size: 6773
DraIII CAC_nnn'GTG
Cuts at: 2428 2428
Size: 6773
DrdI GACnn_nn'nnGTC
Cuts at: 1773 4479 5337 1773
Size: 2706 858 3209
Fragments arranged by size:
3209 2706 858
EaeI y'GGCC_r
Cuts at: 1025 1553 1985 4358 4532 4923 4950 6295 1025
Size: 528 432 2373 174 391 27 1345 1503
Fragments arranged by size:
2373 1503 1345 528 432 391 174 27
EagI C'GGCC_G
Cuts at: 4358 4358
Size: 6773
EarI CTCTTCn'nnn_
Cuts at: 466 881 1364 1433 1959 2558 3535 4796 5006
Size: 415 483 69 526 599 977 1261 210
Cuts at: 5006 466
Size: 2233
Fragments arranged by size:
2233 1261 977 599 526 483 415 210
69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1930 4981 5291 5437 6583 1930
Size: 3051 310 146 1146 2120
Fragments arranged by size:
3051 2120 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1005 3785 1005
Size: 2780 3993
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 374 472 866 1154 1265 1837 3082 3242 3759
Size: 98 394 288 111 572 1245 160 517
Cuts at: 3759 4598 5030 5777 6045 374
Size: 839 432 747 268 1102
Fragments arranged by size:
1245 1102 839 747 572 517 432 394
288 268 160 111 98
EcoNI CCTnn'n_nnAGG
Cuts at: 1915 1915
Size: 6773
EcoO109I rG'GnC_Cy
Cuts at: 157 249 740 1413 2509 3002 3003 3018 3912
Size: 92 491 673 1096 493 1 15 894
Cuts at: 3912 157
Size: 3018
Fragments arranged by size:
3018 1096 894 673 493 491 92 15
1
EcoRI G'AATT_C
Cuts at: 3777 3777
Size: 6773
EcoRV GAT'ATC
Cuts at: 772 2433 772
Size: 1661 5112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 477 1749 4792 477
Size: 1272 3043 2458
Fragments arranged by size:
3043 2458 1272
FspI TGC'GCA
Cuts at: 4554 4554
Size: 6773
HaeII r_GCGC'y
Cuts at: 1278 4207 4455 5477 6456 1278
Size: 2929 248 1022 979 1595
Fragments arranged by size:
2929 1595 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 125 157 175 207 774 780 806 812 1864
Size: 32 18 32 567 6 26 6 1052
Cuts at: 1864 1896 2358 2390 2914 2946 4615 4645 4647
Size: 32 462 32 524 32 1669 30 2
Cuts at: 4647 4677 125
Size: 30 2221
Fragments arranged by size:
2221 1669 1052 567 524 462 32 32
32 32 32 30 30 26 18 6
6 2
HincII GTy'rAC
Cuts at: 1638 3812 1638
Size: 2174 4599
HindIII A'AGCT_T
Cuts at: 557 557
Size: 6773
KasI G'GCGC_C
Cuts at: 4203 4451 4203
Size: 248 6525
KpnI G_GTAC'C
Cuts at: 3793 3793
Size: 6773
MfeI C'AATT_G
Cuts at: 1825 1825
Size: 6773
MluI A'CGCG_T
Cuts at: 6206 6206
Size: 6773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 128 488 2138 3305 3783 5444 5628 128
Size: 360 1650 1167 478 1661 184 1273
Fragments arranged by size:
1661 1650 1273 1167 478 360 184
MscI TGG'CCA
Cuts at: 1027 1555 1987 4534 1027
Size: 528 432 2547 3266
Fragments arranged by size:
3266 2547 528 432
MslI CAynn'nnrTG
Cuts at: 112 1480 2189 2191 2854 2977 3094 4762 4889
Size: 1368 709 2 663 123 117 1668 127
Cuts at: 4889 112
Size: 1996
Fragments arranged by size:
1996 1668 1368 709 663 127 123 117
2
MspA1I CmG'CkG
Cuts at: 163 957 1587 2988 3884 4198 4558 5571 5816
Size: 794 630 1401 896 314 360 1013 245
Cuts at: 5816 163
Size: 1120
Fragments arranged by size:
1401 1120 1013 896 794 630 360 314
245
NaeI GCC'GGC
Cuts at: 4954 4954
Size: 6773
NarI GG'CG_CC
Cuts at: 4204 4452 4204
Size: 248 6525
NcoI C'CATG_G
Cuts at: 107 125 1613 2909 2978 3286 107
Size: 18 1488 1296 69 308 3594
Fragments arranged by size:
3594 1488 1296 308 69 18
NgoMIV G'CCGG_C
Cuts at: 4952 4952
Size: 6773
NheI G'CTAG_C
Cuts at: 3243 3243
Size: 6773
NspI r_CATG'y
Cuts at: 868 1732 3594 3826 4326 5233 868
Size: 864 1862 232 500 907 2408
Fragments arranged by size:
2408 1862 907 864 500 232
PasI CC'CwG_GG
Cuts at: 2505 2794 2505
Size: 289 6484
PciI A'CATG_T
Cuts at: 5229 5229
Size: 6773
PflMI CCAn_nnn'nTGG
Cuts at: 1911 2031 2730 3287 3501 3567 3737 1911
Size: 120 699 557 214 66 170 4947
Fragments arranged by size:
4947 699 557 214 170 120 66
PfoI T'CCnGG_A
Cuts at: 736 778 3259 736
Size: 42 2481 4250
Fragments arranged by size:
4250 2481 42
PmeI GTTT'AAAC
Cuts at: 3772 3772
Size: 6773
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5912 5944 5912
Size: 32 6741
PpuMI rG'GwC_Cy
Cuts at: 249 740 1413 2509 249
Size: 491 673 1096 4513
Fragments arranged by size:
4513 1096 673 491
PsiI TTA'TAA
Cuts at: 911 911
Size: 6773
PspOMI G'GGCC_C
Cuts at: 3002 3329 3002
Size: 327 6446
PspXI vC'TCGA_Gb
Cuts at: 181 181
Size: 6773
PstI C_TGCA'G
Cuts at: 3112 3433 3820 4505 3112
Size: 321 387 685 5380
Fragments arranged by size:
5380 685 387 321
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6773
PvuII CAG'CTG
Cuts at: 957 2988 4198 4558 957
Size: 2031 1210 360 3172
Fragments arranged by size:
3172 2031 1210 360
RsrII CG'GwC_CG
Cuts at: 4968 4968
Size: 6773
SacI G_AGCT'C
Cuts at: 1007 3787 1007
Size: 2780 3993
SacII CC_GC'GG
Cuts at: 164 164
Size: 6773
SalI G'TCGA_C
Cuts at: 3810 3810
Size: 6773
SapI GCTCTTCn'nnn_
Cuts at: 881 1433 2558 4796 5006 881
Size: 552 1125 2238 210 2648
Fragments arranged by size:
2648 2238 1125 552 210
SbfI CC_TGCA'GG
Cuts at: 3820 3820
Size: 6773
ScaI AGT'ACT
Cuts at: 6290 6290
Size: 6773
SexAI A'CCwGG_T
Cuts at: 2424 2424
Size: 6773
SfcI C'TryA_G
Cuts at: 33 119 3108 3162 3429 3816 4501 5494 5685
Size: 86 2989 54 267 387 685 993 191
Cuts at: 5685 33
Size: 1121
Fragments arranged by size:
2989 1121 993 685 387 267 191 86
54
SfoI GGC'GCC
Cuts at: 4205 4453 4205
Size: 248 6525
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6773
SmaI CCC'GGG
Cuts at: 745 3795 745
Size: 3050 3723
SmlI C'TyrA_G
Cuts at: 181 1417 1783 2902 3749 3928 4843 5335 5597
Size: 1236 366 1119 847 179 915 492 262
Cuts at: 5597 181
Size: 1357
Fragments arranged by size:
1357 1236 1119 915 847 492 366 262
179
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6773
SphI G_CATG'C
Cuts at: 1732 3826 4326 1732
Size: 2094 500 4179
Fragments arranged by size:
4179 2094 500
StuI AGG'CCT
Cuts at: 986 986
Size: 6773
StyI C'CwwG_G
Cuts at: 107 125 1550 1613 2264 2909 2978 3170 3286
Size: 18 1425 63 651 645 69 192 116
Cuts at: 3286 3581 3907 4261 107
Size: 295 326 354 2619
Fragments arranged by size:
2619 1425 651 645 354 326 295 192
116 69 63 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 764 1918 1937 3540 4356 4489 4948 5157 764
Size: 1154 19 1603 816 133 459 209 2380
Fragments arranged by size:
2380 1603 1154 816 459 209 133 19
TatI w'GTAC_w
Cuts at: 2084 2348 2543 2813 2990 3251 3710 6288 2084
Size: 264 195 270 177 261 459 2578 2569
Fragments arranged by size:
2578 2569 459 270 264 261 195 177
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6290 6322 6290
Size: 32 6741
Tth111I GACn'n_nGTC
Cuts at: 4570 4570
Size: 6773
XbaI T'CTAG_A
Cuts at: 766 3804 766
Size: 3038 3735
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4268 4268
Size: 6773
XhoI C'TCGA_G
Cuts at: 181 3749 181
Size: 3568 3205
XmaI C'CCGG_G
Cuts at: 743 3793 743
Size: 3050 3723
XmnI GAAnn'nnTTC
Cuts at: 1352 3157 1352
Size: 1805 4968
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PsiI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AscI AvrII BfrBI BglI
BmgBI BsiWI BssHII BstZ17I ClaI CspCI FseI FspAI
HpaI NdeI NotI NruI NsiI PacI PmlI PshAI
PsrI SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
ZraI