Restriction map for
pF1KB0356
(Circular) (Six-base) MAPSORT of: pF1KB0356.seq Check: 3555 from: 1 to: 6317
pF1KB0356 6317 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 727 3355 727
Size: 2628 3689
Acc65I G'GTAC_C
Cuts at: 3333 3333
Size: 6317
AclI AA'CG_TT
Cuts at: 542 3311 6157 542
Size: 2769 2846 702
Fragments arranged by size:
2846 2769 702
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 794 2089 4142 4574 4927 5589 794
Size: 1295 2053 432 353 662 1522
Fragments arranged by size:
2053 1522 1295 662 432 353
AflIII A'CryG_T
Cuts at: 2800 2958 3669 5469 5750 2800
Size: 158 711 1800 281 3367
Fragments arranged by size:
3367 1800 711 281 158
AhdI GACnn_n'nnGTC
Cuts at: 2472 2472
Size: 6317
AleI CACnn'nnGTG
Cuts at: 1778 1778
Size: 6317
AlwNI CAG_nnn'CTG
Cuts at: 3622 5060 3622
Size: 1438 4879
ApaLI G'TGCA_C
Cuts at: 1103 2711 5155 1103
Size: 1608 2444 2265
Fragments arranged by size:
2444 2265 1608
ApoI r'AATT_y
Cuts at: 349 487 1118 1796 1830 2664 2684 2938 3034
Size: 138 631 678 34 834 20 254 96
Cuts at: 3034 3321 5670 5926 349
Size: 287 2349 256 740
Fragments arranged by size:
2349 834 740 678 631 287 256 254
138 96 34 20
AseI AT'TA_AT
Cuts at: 20 232 20
Size: 212 6105
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6317
AvaI C'yCGr_G
Cuts at: 1610 3337 1610
Size: 1727 4590
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1400 1433 1400
Size: 33 6284
BamHI G'GATC_C
Cuts at: 3342 3342
Size: 6317
BanI G'GyrC_C
Cuts at: 3333 3747 3995 4030 3333
Size: 414 248 35 5620
Fragments arranged by size:
5620 414 248 35
BanII G_rGCy'C
Cuts at: 3012 3331 4361 3012
Size: 319 1030 4968
Fragments arranged by size:
4968 1030 319
BbeI G_GCGC'C
Cuts at: 3751 3999 3751
Size: 248 6069
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6283
BciVI GTATCCnnnnn_n'
Cuts at: 1617 2809 4215 5271 6317 1617
Size: 1192 1406 1056 1046 1617
Fragments arranged by size:
1617 1406 1192 1056 1046
BclI T'GATC_A
Cuts at: 3837 3837
Size: 6317
BfrBI ATG'CAT
Cuts at: 3304 3304
Size: 6317
BglII A'GATC_T
Cuts at: 1166 3832 5869 1166
Size: 2666 2037 1614
Fragments arranged by size:
2666 2037 1614
BlpI GC'TnA_GC
Cuts at: 3429 3429
Size: 6317
Bme1580I G_kGCm'C
Cuts at: 1107 2102 2715 3942 4035 5159 1107
Size: 995 613 1227 93 1124 2265
Fragments arranged by size:
2265 1227 1124 995 613 93
BmrI ACTGGGnnnn_n'
Cuts at: 3529 3610 3945 6089 3529
Size: 81 335 2144 3757
Fragments arranged by size:
3757 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1741 4684 1741
Size: 2943 3374
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 3493 4372 5086 5384 314
Size: 3179 879 714 298 1247
Fragments arranged by size:
3179 1247 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 6021 6021
Size: 6317
BsaAI yAC'GTr
Cuts at: 1137 3670 1137
Size: 2533 3784
BsaBI GATnn'nnATC
Cuts at: 6 3245 3831 5868 6
Size: 3239 586 2037 455
Fragments arranged by size:
3239 2037 586 455
BsaHI Gr'CG_yC
Cuts at: 3748 3996 4698 6194 3748
Size: 248 702 1496 3871
Fragments arranged by size:
3871 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3506 4027 4705 5116 5263 5537 3506
Size: 521 678 411 147 274 4286
Fragments arranged by size:
4286 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1211 2357 1211
Size: 1146 5171
BseYI C'CCAG_C
Cuts at: 3742 4500 5165 5573 3742
Size: 758 665 408 4486
Fragments arranged by size:
4486 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 3859 3905 5135 104
Size: 3755 46 1230 1286
Fragments arranged by size:
3755 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1107 2715 3331 4109 4299 5159 1107
Size: 1608 616 778 190 860 2265
Fragments arranged by size:
2265 1608 860 778 616 190
BsmI GAATG_Cn'
Cuts at: 217 3022 3140 217
Size: 2805 118 3394
Fragments arranged by size:
3394 2805 118
Bsp1286I G_dGCh'C
Cuts at: 1107 2102 2715 3012 3331 3942 4035 4109 4299
Size: 995 613 297 319 611 93 74 190
Cuts at: 4299 4361 5159 1107
Size: 62 798 2265
Fragments arranged by size:
2265 995 798 613 611 319 297 190
93 74 62
BspHI T'CATG_A
Cuts at: 3224 4749 3224
Size: 1525 4792
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3367 3581 3883 4264 3367
Size: 214 302 381 5420
Fragments arranged by size:
5420 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4609 4663 6147 6312 50
Size: 4559 54 1484 165 55
Fragments arranged by size:
4559 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 602 2237 3161 4229 602
Size: 1635 924 1068 2690
Fragments arranged by size:
2690 1635 1068 924
BsrFI r'CCGG_y
Cuts at: 4315 4496 4315
Size: 181 6136
BssSI C'ACGA_G
Cuts at: 1298 1715 4588 5296 1298
Size: 417 2873 708 2319
Fragments arranged by size:
2873 2319 708 417
BstAPI GCAn_nnn'nTGC
Cuts at: 2538 3694 2538
Size: 1156 5161
BstBI TT'CG_AA
Cuts at: 1176 2601 3174 4678 1176
Size: 1425 573 1504 2815
Fragments arranged by size:
2815 1504 1425 573
BstXI CCAn_nnnn'nTGG
Cuts at: 1376 2158 1376
Size: 782 5535
BstYI r'GATC_y
Cuts at: 1 440 504 561 1166 1719 1929 3278 3342
Size: 439 64 57 605 553 210 1349 64
Cuts at: 3342 3809 3832 4167 4413 4719 4731 4817 5869
Size: 467 23 335 246 306 12 86 1052
Cuts at: 5869 1
Size: 449
Fragments arranged by size:
1349 1052 605 553 467 449 439 335
306 246 210 86 64 64 57 23
12
BstZ17I GTA'TAC
Cuts at: 728 728
Size: 6317
Bsu36I CC'TnA_GG
Cuts at: 2398 2398
Size: 6317
BtgI C'CryG_G
Cuts at: 1191 1191
Size: 6317
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4267 4448 4611 4267
Size: 181 163 5973
Fragments arranged by size:
5973 181 163
BtsI GCAGTG_nn'
Cuts at: 1557 2713 3560 1557
Size: 1156 847 4314
Fragments arranged by size:
4314 1156 847
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2429 2464 2429
Size: 35 6282
DraI TTT'AAA
Cuts at: 258 404 863 2120 2511 3316 258
Size: 146 459 1257 391 805 3259
Fragments arranged by size:
3259 1257 805 459 391 146
DrdI GACnn_nn'nnGTC
Cuts at: 4023 5367 4023
Size: 1344 4973
EaeI y'GGCC_r
Cuts at: 1444 3902 4076 4467 4494 5839 1444
Size: 2458 174 391 27 1345 1922
Fragments arranged by size:
2458 1922 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3902 3902
Size: 6317
EarI CTCTTCn'nnn_
Cuts at: 1580 2402 3103 4340 4550 1580
Size: 822 701 1237 210 3347
Fragments arranged by size:
3347 1237 822 701 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4525 5267 5413 6127 4525
Size: 742 146 714 4715
Fragments arranged by size:
4715 742 714 146
EcoICRI GAG'CTC
Cuts at: 3329 3329
Size: 6317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 794 2089 4142 4574 4927 5589 794
Size: 1295 2053 432 353 662 1522
Fragments arranged by size:
2053 1522 1295 662 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 220 220
Size: 6317
EcoO109I rG'GnC_Cy
Cuts at: 570 2019 3456 570
Size: 1449 1437 3431
Fragments arranged by size:
3431 1449 1437
EcoRI G'AATT_C
Cuts at: 1830 3321 1830
Size: 1491 4826
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1000 1253 4336 1000
Size: 253 3083 2981
Fragments arranged by size:
3083 2981 253
FspI TGC'GCA
Cuts at: 4098 4098
Size: 6317
HaeII r_GCGC'y
Cuts at: 3751 3999 5229 6000 3751
Size: 248 1230 771 4068
Fragments arranged by size:
4068 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 432 464 1711 1743 2458 2490 4159 4189 4191
Size: 32 1247 32 715 32 1669 30 2
Cuts at: 4191 4221 432
Size: 30 2528
Fragments arranged by size:
2528 1669 1247 715 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3356 3356
Size: 6317
KasI G'GCGC_C
Cuts at: 3747 3995 3747
Size: 248 6069
KpnI G_GTAC'C
Cuts at: 3337 3337
Size: 6317
MfeI C'AATT_G
Cuts at: 1596 1596
Size: 6317
MluI A'CGCG_T
Cuts at: 5750 5750
Size: 6317
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1511 5076 5260 1511
Size: 3565 184 2568
Fragments arranged by size:
3565 2568 184
MscI TGG'CCA
Cuts at: 1446 4078 1446
Size: 2632 3685
MslI CAynn'nnrTG
Cuts at: 478 749 1374 1778 2677 2805 2872 3251 4306
Size: 271 625 404 899 128 67 379 1055
Cuts at: 4306 4433 478
Size: 127 2362
Fragments arranged by size:
2362 1055 899 625 404 379 271 128
127 67
MspA1I CmG'CkG
Cuts at: 3428 3742 4102 4886 5131 3428
Size: 314 360 784 245 4614
Fragments arranged by size:
4614 784 360 314 245
NaeI GCC'GGC
Cuts at: 4498 4498
Size: 6317
NarI GG'CG_CC
Cuts at: 3748 3996 3748
Size: 248 6069
NcoI C'CATG_G
Cuts at: 1191 1191
Size: 6317
NgoMIV G'CCGG_C
Cuts at: 4496 4496
Size: 6317
NsiI A_TGCA'T
Cuts at: 3306 3306
Size: 6317
NspI r_CATG'y
Cuts at: 477 1594 2192 3091 3304 3370 3870 5473 477
Size: 1117 598 899 213 66 500 1603 1321
Fragments arranged by size:
1603 1321 1117 899 598 500 213 66
PciI A'CATG_T
Cuts at: 5469 5469
Size: 6317
PmeI GTTT'AAAC
Cuts at: 3316 3316
Size: 6317
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4763 4795 4763
Size: 32 6285
PpuMI rG'GwC_Cy
Cuts at: 570 2019 570
Size: 1449 4868
PsiI TTA'TAA
Cuts at: 1096 2948 1096
Size: 1852 4465
PstI C_TGCA'G
Cuts at: 2720 3364 4049 2720
Size: 644 685 4988
Fragments arranged by size:
4988 685 644
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6317
PvuII CAG'CTG
Cuts at: 3742 4102 3742
Size: 360 5957
RsrII CG'GwC_CG
Cuts at: 4512 4512
Size: 6317
SacI G_AGCT'C
Cuts at: 3331 3331
Size: 6317
SalI G'TCGA_C
Cuts at: 3354 3354
Size: 6317
SapI GCTCTTCn'nnn_
Cuts at: 1580 4340 4550 1580
Size: 2760 210 3347
Fragments arranged by size:
3347 2760 210
SbfI CC_TGCA'GG
Cuts at: 3364 3364
Size: 6317
ScaI AGT'ACT
Cuts at: 5834 5834
Size: 6317
SfcI C'TryA_G
Cuts at: 33 2350 2716 3360 4045 5013 5204 33
Size: 2317 366 644 685 968 191 1146
Fragments arranged by size:
2317 1146 968 685 644 366 191
SfoI GGC'GCC
Cuts at: 3749 3997 3749
Size: 248 6069
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6317
SmaI CCC'GGG
Cuts at: 3339 3339
Size: 6317
SmlI C'TyrA_G
Cuts at: 329 3472 4387 5101 5363 329
Size: 3143 915 714 262 1283
Fragments arranged by size:
3143 1283 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6317
SphI G_CATG'C
Cuts at: 477 1594 3091 3304 3370 3870 477
Size: 1117 1497 213 66 500 2924
Fragments arranged by size:
2924 1497 1117 500 213 66
SspI AAT'ATT
Cuts at: 534 1149 1972 2038 534
Size: 615 823 66 4813
Fragments arranged by size:
4813 823 615 66
StyI C'CwwG_G
Cuts at: 1191 3451 3805 1191
Size: 2260 354 3703
Fragments arranged by size:
3703 2260 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3900 4033 4492 4701 3900
Size: 133 459 209 5516
Fragments arranged by size:
5516 459 209 133
TatI w'GTAC_w
Cuts at: 513 2013 5832 513
Size: 1500 3819 998
Fragments arranged by size:
3819 1500 998
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 185 217 5834 5866 185
Size: 32 5617 32 636
Fragments arranged by size:
5617 636 32 32
Tth111I GACn'n_nGTC
Cuts at: 4114 4114
Size: 6317
XbaI T'CTAG_A
Cuts at: 1834 3348 1834
Size: 1514 4803
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3812 3812
Size: 6317
XmaI C'CCGG_G
Cuts at: 3337 3337
Size: 6317
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PmeI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AlfI AloI ApaI
AscI AvrII BbsI BbvCI BglI BmgBI BmtI BpmI
Bpu10I BsaXI BsgI BsiWI BsmBI BspEI BsrGI BssHII
BstEII ClaI DraIII EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NruI PacI PasI PflMI PfoI
PmlI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI
ZraI