Restriction map for
pF1KB0401
(Circular) (Six-base) MAPSORT of: pF1KB0401.seq Check: 3892 from: 1 to: 3626
pF1KB0401 3626 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AccI GT'mk_AC
Cuts at: 664 664
Size: 3626
Acc65I G'GTAC_C
Cuts at: 642 642
Size: 3626
AclI AA'CG_TT
Cuts at: 3466 3466
Size: 3626
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 422 1451 1883 2630 2898 422
Size: 1029 432 747 268 1150
Fragments arranged by size:
1150 1029 747 432 268
AflIII A'CryG_T
Cuts at: 978 2082 3059 978
Size: 1104 977 1545
Fragments arranged by size:
1545 1104 977
AgeI A'CCGG_T
Cuts at: 2014 2014
Size: 3626
AlwNI CAG_nnn'CTG
Cuts at: 400 931 2498 400
Size: 531 1567 1528
Fragments arranged by size:
1567 1528 531
ApaLI G'TGCA_C
Cuts at: 2396 2396
Size: 3626
ApoI r'AATT_y
Cuts at: 561 630 2979 3235 561
Size: 69 2349 256 952
Fragments arranged by size:
2349 952 256 69
AseI AT'TA_AT
Cuts at: 20 20
Size: 3626
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3626
AvaI C'yCGr_G
Cuts at: 646 646
Size: 3626
BamHI G'GATC_C
Cuts at: 651 651
Size: 3626
BanI G'GyrC_C
Cuts at: 451 642 1056 1304 1339 451
Size: 191 414 248 35 2738
Fragments arranged by size:
2738 414 248 191 35
BanII G_rGCy'C
Cuts at: 640 1670 640
Size: 1030 2596
BbeI G_GCGC'C
Cuts at: 1060 1308 1060
Size: 248 3378
BbvCI CC'TCA_GC
Cuts at: 149 149
Size: 3626
BciVI GTATCCnnnnn_n'
Cuts at: 1524 2285 3626 1524
Size: 761 1341 1524
Fragments arranged by size:
1524 1341 761
BclI T'GATC_A
Cuts at: 228 1146 228
Size: 918 2708
BglII A'GATC_T
Cuts at: 1141 3178 1141
Size: 2037 1589
BlpI GC'TnA_GC
Cuts at: 738 738
Size: 3626
Bme1580I G_kGCm'C
Cuts at: 1251 1344 2400 1251
Size: 93 1056 2477
Fragments arranged by size:
2477 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 838 919 1254 3398 838
Size: 81 335 2144 1066
Fragments arranged by size:
2144 1066 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1993 1993
Size: 3626
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 320
Size: 3626
Bpu10I CC'TnA_GC
Cuts at: 149 149
Size: 3626
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 492 802 1681 2173 2471 492
Size: 310 879 492 298 1647
Fragments arranged by size:
1647 879 492 310 298
BsaI GGTCTCn'nnnn_
Cuts at: 3330 3330
Size: 3626
BsaAI yAC'GTr
Cuts at: 979 979
Size: 3626
BsaBI GATnn'nnATC
Cuts at: 6 308 1140 3177 6
Size: 302 832 2037 455
Fragments arranged by size:
2037 832 455 302
BsaHI Gr'CG_yC
Cuts at: 1057 1305 2007 3503 1057
Size: 248 702 1496 1180
Fragments arranged by size:
1496 1180 702 248
BsaWI w'CCGG_w
Cuts at: 815 1336 2014 2288 2435 2846 815
Size: 521 678 274 147 411 1595
Fragments arranged by size:
1595 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1051 1809 2386 2882 1051
Size: 758 577 496 1795
Fragments arranged by size:
1795 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1168 1214 2422 104
Size: 1064 46 1208 1308
Fragments arranged by size:
1308 1208 1064 46
BsiHKAI G_wGCw'C
Cuts at: 640 1418 1608 2400 640
Size: 778 190 792 1866
Fragments arranged by size:
1866 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 640 1251 1344 1418 1608 1670 2400 640
Size: 611 93 74 190 62 730 1866
Fragments arranged by size:
1866 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2846 2846
Size: 3626
BspHI T'CATG_A
Cuts at: 2802 2802
Size: 3626
BspMI ACCTGCnnnn'nnnn_
Cuts at: 676 890 1192 1573 676
Size: 214 302 381 2729
Fragments arranged by size:
2729 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1918 1972 3456 3621 50
Size: 1868 54 1484 165 55
Fragments arranged by size:
1868 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1538 1538
Size: 3626
BsrFI r'CCGG_y
Cuts at: 1624 1805 2014 1624
Size: 181 209 3236
Fragments arranged by size:
3236 209 181
BsrGI T'GTAC_A
Cuts at: 530 530
Size: 3626
BssSI C'ACGA_G
Cuts at: 1897 2255 1897
Size: 358 3268
BstAPI GCAn_nnn'nTGC
Cuts at: 273 1003 273
Size: 730 2896
BstBI TT'CG_AA
Cuts at: 1987 1987
Size: 3626
BstYI r'GATC_y
Cuts at: 1 396 651 1118 1141 1476 1722 2734 2820
Size: 395 255 467 23 335 246 1012 86
Cuts at: 2820 2832 3178 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 395 346 335 255 246
86 23 12
BtgI C'CryG_G
Cuts at: 491 491
Size: 3626
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1576 1757 1920 1576
Size: 181 163 3282
Fragments arranged by size:
3282 181 163
BtsI GCAGTG_nn'
Cuts at: 869 869
Size: 3626
DraI TTT'AAA
Cuts at: 625 625
Size: 3626
DrdI GACnn_nn'nnGTC
Cuts at: 1332 2190 1332
Size: 858 2768
EaeI y'GGCC_r
Cuts at: 1211 1385 1776 1803 3148 1211
Size: 174 391 27 1345 1689
Fragments arranged by size:
1689 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1211 1211
Size: 3626
EarI CTCTTCn'nnn_
Cuts at: 1649 1859 1649
Size: 210 3416
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1834 2144 2290 3436 1834
Size: 310 146 1146 2024
Fragments arranged by size:
2024 1146 310 146
EcoICRI GAG'CTC
Cuts at: 638 638
Size: 3626
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 422 1451 1883 2630 2898 320
Size: 102 1029 432 747 268 1048
Fragments arranged by size:
1048 1029 747 432 268 102
EcoO109I rG'GnC_Cy
Cuts at: 340 765 340
Size: 425 3201
EcoRI G'AATT_C
Cuts at: 630 630
Size: 3626
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1645 1645
Size: 3626
FspI TGC'GCA
Cuts at: 1407 1407
Size: 3626
HaeII r_GCGC'y
Cuts at: 147 1060 1308 2330 3309 147
Size: 913 248 1022 979 464
Fragments arranged by size:
1022 979 913 464 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1468 1498 1500 1530 1468
Size: 30 2 30 3564
Fragments arranged by size:
3564 30 30 2
HincII GTy'rAC
Cuts at: 665 665
Size: 3626
KasI G'GCGC_C
Cuts at: 1056 1304 1056
Size: 248 3378
KpnI G_GTAC'C
Cuts at: 646 646
Size: 3626
MluI A'CGCG_T
Cuts at: 3059 3059
Size: 3626
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 322 2297 2481 322
Size: 1975 184 1467
Fragments arranged by size:
1975 1467 184
MscI TGG'CCA
Cuts at: 1387 1387
Size: 3626
MslI CAynn'nnrTG
Cuts at: 1615 1742 1615
Size: 127 3499
MspA1I CmG'CkG
Cuts at: 737 1051 1411 2424 2669 737
Size: 314 360 1013 245 1694
Fragments arranged by size:
1694 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1807 1807
Size: 3626
NarI GG'CG_CC
Cuts at: 1057 1305 1057
Size: 248 3378
NcoI C'CATG_G
Cuts at: 491 491
Size: 3626
NgoMIV G'CCGG_C
Cuts at: 1805 1805
Size: 3626
NspI r_CATG'y
Cuts at: 679 1179 2086 679
Size: 500 907 2219
Fragments arranged by size:
2219 907 500
PciI A'CATG_T
Cuts at: 2082 2082
Size: 3626
PmeI GTTT'AAAC
Cuts at: 625 625
Size: 3626
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2765 2797 2765
Size: 32 3594
PstI C_TGCA'G
Cuts at: 673 1358 673
Size: 685 2941
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3626
PvuII CAG'CTG
Cuts at: 1051 1411 1051
Size: 360 3266
RsrII CG'GwC_CG
Cuts at: 1821 1821
Size: 3626
SacI G_AGCT'C
Cuts at: 640 640
Size: 3626
SalI G'TCGA_C
Cuts at: 663 663
Size: 3626
SapI GCTCTTCn'nnn_
Cuts at: 1649 1859 1649
Size: 210 3416
SbfI CC_TGCA'GG
Cuts at: 673 673
Size: 3626
ScaI AGT'ACT
Cuts at: 3143 3143
Size: 3626
SfcI C'TryA_G
Cuts at: 33 669 1354 2347 2538 33
Size: 636 685 993 191 1121
Fragments arranged by size:
1121 993 685 636 191
SfoI GGC'GCC
Cuts at: 1058 1306 1058
Size: 248 3378
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3626
SmaI CCC'GGG
Cuts at: 648 648
Size: 3626
SmlI C'TyrA_G
Cuts at: 471 781 1696 2188 2450 471
Size: 310 915 492 262 1647
Fragments arranged by size:
1647 915 492 310 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3626
SphI G_CATG'C
Cuts at: 679 1179 679
Size: 500 3126
StuI AGG'CCT
Cuts at: 214 214
Size: 3626
StyI C'CwwG_G
Cuts at: 491 760 1114 491
Size: 269 354 3003
Fragments arranged by size:
3003 354 269
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1209 1342 1801 2010 1209
Size: 133 459 209 2825
Fragments arranged by size:
2825 459 209 133
TatI w'GTAC_w
Cuts at: 530 3141 530
Size: 2611 1015
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3143 3175 3143
Size: 32 3594
Tth111I GACn'n_nGTC
Cuts at: 1423 1423
Size: 3626
XbaI T'CTAG_A
Cuts at: 312 657 312
Size: 345 3281
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1121 1121
Size: 3626
XmaI C'CCGG_G
Cuts at: 646 646
Size: 3626
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BglI
BmgBI BmtI BsaXI BseRI BsgI BsiWI BsmI BsmBI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI