Restriction map for
pF1KB0407
(Circular) (Six-base) MAPSORT of: pF1KB0407.seq Check: 2262 from: 1 to: 4508
pF1KB0407 4508 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 619 1168 619
Size: 549 3959
AatII G_ACGT'C
Cuts at: 839 839
Size: 4508
AccI GT'mk_AC
Cuts at: 1546 1546
Size: 4508
Acc65I G'GTAC_C
Cuts at: 1049 1524 1049
Size: 475 4033
AclI AA'CG_TT
Cuts at: 4348 4348
Size: 4508
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1002 2333 2765 3512 3780 1002
Size: 1331 432 747 268 1730
Fragments arranged by size:
1730 1331 747 432 268
AflIII A'CryG_T
Cuts at: 1860 2964 3941 1860
Size: 1104 977 2427
Fragments arranged by size:
2427 1104 977
AgeI A'CCGG_T
Cuts at: 1046 2896 1046
Size: 1850 2658
AhdI GACnn_n'nnGTC
Cuts at: 641 641
Size: 4508
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1079 1079
Size: 4508
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1477 1509 1477
Size: 32 4476
AlwNI CAG_nnn'CTG
Cuts at: 611 1813 3380 611
Size: 1202 1567 1739
Fragments arranged by size:
1739 1567 1202
ApaLI G'TGCA_C
Cuts at: 828 3278 828
Size: 2450 2058
ApoI r'AATT_y
Cuts at: 727 1512 3861 4117 727
Size: 785 2349 256 1118
Fragments arranged by size:
2349 1118 785 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4508
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4508
AvaI C'yCGr_G
Cuts at: 416 1435 1528 416
Size: 1019 93 3396
Fragments arranged by size:
3396 1019 93
BamHI G'GATC_C
Cuts at: 1533 1533
Size: 4508
BanI G'GyrC_C
Cuts at: 1049 1524 1938 2186 2221 1049
Size: 475 414 248 35 3336
Fragments arranged by size:
3336 475 414 248 35
BanII G_rGCy'C
Cuts at: 404 847 1010 1522 2552 404
Size: 443 163 512 1030 2360
Fragments arranged by size:
2360 1030 512 443 163
BbeI G_GCGC'C
Cuts at: 1942 2190 1942
Size: 248 4260
BbvCI CC'TCA_GC
Cuts at: 256 427 256
Size: 171 4337
BciVI GTATCCnnnnn_n'
Cuts at: 2406 3167 4508 2406
Size: 761 1341 2406
Fragments arranged by size:
2406 1341 761
BclI T'GATC_A
Cuts at: 2028 2028
Size: 4508
BglI GCCn_nnn'nGGC
Cuts at: 1306 1306
Size: 4508
BglII A'GATC_T
Cuts at: 2023 4060 2023
Size: 2037 2471
BlpI GC'TnA_GC
Cuts at: 200 1620 200
Size: 1420 3088
Bme1580I G_kGCm'C
Cuts at: 832 2133 2226 3282 832
Size: 1301 93 1056 2058
Fragments arranged by size:
2058 1301 1056 93
BmgBI CAC'GTC
Cuts at: 1500 1500
Size: 4508
BmrI ACTGGGnnnn_n'
Cuts at: 142 1419 1720 1801 2136 4280 142
Size: 1277 301 81 335 2144 370
Fragments arranged by size:
2144 1277 370 335 301 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 431 2875 431
Size: 2444 2064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 117 377 117
Size: 260 4248
Bpu10I CC'TnA_GC
Cuts at: 256 427 256
Size: 171 4337
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 1684 2563 3055 3353 169
Size: 1515 879 492 298 1324
Fragments arranged by size:
1515 1324 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 315 4212 315
Size: 3897 611
BsaAI yAC'GTr
Cuts at: 827 879 1861 827
Size: 52 982 3474
Fragments arranged by size:
3474 982 52
BsaBI GATnn'nnATC
Cuts at: 6 2022 4059 6
Size: 2016 2037 455
Fragments arranged by size:
2037 2016 455
BsaHI Gr'CG_yC
Cuts at: 836 1939 2187 2889 4385 836
Size: 1103 248 702 1496 959
Fragments arranged by size:
1496 1103 959 702 248
BsaWI w'CCGG_w
Cuts at: 677 1046 1697 2218 2896 3170 3317 3728 677
Size: 369 651 521 678 274 147 411 1457
Fragments arranged by size:
1457 678 651 521 411 369 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 321 351 440 470 321
Size: 30 89 30 4359
Fragments arranged by size:
4359 89 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1084 1084
Size: 4508
BseYI C'CCAG_C
Cuts at: 653 1933 2691 3268 3764 653
Size: 1280 758 577 496 1397
Fragments arranged by size:
1397 1280 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 337 460 598 624 743 1085 1257 1449
Size: 42 123 138 26 119 342 172 192
Cuts at: 1449 295
Size: 3354
Fragments arranged by size:
3354 342 192 172 138 123 119 42
26
BsiEI CG_ry'CG
Cuts at: 104 2050 2096 3304 104
Size: 1946 46 1208 1308
Fragments arranged by size:
1946 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 750 832 1010 1522 2300 2490 3282 750
Size: 82 178 512 778 190 792 1976
Fragments arranged by size:
1976 792 778 512 190 178 82
BsmI GAATG_Cn'
Cuts at: 354 697 354
Size: 343 4165
Bsp1286I G_dGCh'C
Cuts at: 404 750 832 847 1010 1522 2133 2226 2300
Size: 346 82 15 163 512 611 93 74
Cuts at: 2300 2490 2552 3282 404
Size: 190 62 730 1630
Fragments arranged by size:
1630 730 611 512 346 190 163 93
82 74 62 15
BspEI T'CCGG_A
Cuts at: 3728 3728
Size: 4508
BspHI T'CATG_A
Cuts at: 3684 3684
Size: 4508
BspMI ACCTGCnnnn'nnnn_
Cuts at: 511 619 1168 1558 1772 2074 2455 511
Size: 108 549 390 214 302 381 2564
Fragments arranged by size:
2564 549 390 381 302 214 108
BsrBI CCG'CTC
Cuts at: 50 396 1123 2800 2854 4338 4503 50
Size: 346 727 1677 54 1484 165 55
Fragments arranged by size:
1677 1484 727 346 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1179 2420 1179
Size: 1241 3267
BsrFI r'CCGG_y
Cuts at: 1046 2506 2687 2896 1046
Size: 1460 181 209 2658
Fragments arranged by size:
2658 1460 209 181
BssSI C'ACGA_G
Cuts at: 2779 3137 2779
Size: 358 4150
BstAPI GCAn_nnn'nTGC
Cuts at: 611 827 1885 611
Size: 216 1058 3234
Fragments arranged by size:
3234 1058 216
BstBI TT'CG_AA
Cuts at: 2869 2869
Size: 4508
BstXI CCAn_nnnn'nTGG
Cuts at: 653 1060 653
Size: 407 4101
BstYI r'GATC_y
Cuts at: 1 1533 2000 2023 2358 2604 3616 3702 3714
Size: 1532 467 23 335 246 1012 86 12
Cuts at: 3714 4060 1
Size: 346 449
Fragments arranged by size:
1532 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1257 1257
Size: 4508
BtgI C'CryG_G
Cuts at: 138 325 138
Size: 187 4321
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2458 2639 2802 2458
Size: 181 163 4164
Fragments arranged by size:
4164 181 163
BtsI GCAGTG_nn'
Cuts at: 705 1245 1751 705
Size: 540 506 3462
Fragments arranged by size:
3462 540 506
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 865 900 865
Size: 35 4473
DraI TTT'AAA
Cuts at: 738 1507 738
Size: 769 3739
DraIII CAC_nnn'GTG
Cuts at: 315 315
Size: 4508
DrdI GACnn_nn'nnGTC
Cuts at: 2214 3072 2214
Size: 858 3650
EaeI y'GGCC_r
Cuts at: 2093 2267 2658 2685 4030 2093
Size: 174 391 27 1345 2571
Fragments arranged by size:
2571 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2093 2093
Size: 4508
EarI CTCTTCn'nnn_
Cuts at: 1028 2531 2741 1028
Size: 1503 210 2795
Fragments arranged by size:
2795 1503 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2716 3026 3172 4318 2716
Size: 310 146 1146 2906
Fragments arranged by size:
2906 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1008 1520 1008
Size: 512 3996
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 117 377 1002 2333 2765 3512 3780 117
Size: 260 625 1331 432 747 268 845
Fragments arranged by size:
1331 845 747 625 432 268 260
EcoO109I rG'GnC_Cy
Cuts at: 562 1647 562
Size: 1085 3423
EcoRI G'AATT_C
Cuts at: 1512 1512
Size: 4508
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2527 2527
Size: 4508
FspI TGC'GCA
Cuts at: 2289 2289
Size: 4508
HaeII r_GCGC'y
Cuts at: 1942 2190 3212 4191 1942
Size: 248 1022 979 2259
Fragments arranged by size:
2259 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 321 353 627 659 2350 2380 2382 2412 321
Size: 32 274 32 1691 30 2 30 2417
Fragments arranged by size:
2417 1691 274 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1547 1547
Size: 4508
KasI G'GCGC_C
Cuts at: 1938 2186 1938
Size: 248 4260
KpnI G_GTAC'C
Cuts at: 1053 1528 1053
Size: 475 4033
MluI A'CGCG_T
Cuts at: 3941 3941
Size: 4508
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 787 3179 3363 787
Size: 2392 184 1932
Fragments arranged by size:
2392 1932 184
MscI TGG'CCA
Cuts at: 2269 2269
Size: 4508
MslI CAynn'nnrTG
Cuts at: 112 1405 2497 2624 112
Size: 1293 1092 127 1996
Fragments arranged by size:
1996 1293 1092 127
MspA1I CmG'CkG
Cuts at: 508 1367 1619 1933 2293 3306 3551 508
Size: 859 252 314 360 1013 245 1465
Fragments arranged by size:
1465 1013 859 360 314 252 245
NaeI GCC'GGC
Cuts at: 2689 2689
Size: 4508
NarI GG'CG_CC
Cuts at: 1939 2187 1939
Size: 248 4260
NcoI C'CATG_G
Cuts at: 138 138
Size: 4508
NgoMIV G'CCGG_C
Cuts at: 2687 2687
Size: 4508
NspI r_CATG'y
Cuts at: 1404 1561 2061 2968 1404
Size: 157 500 907 2944
Fragments arranged by size:
2944 907 500 157
PasI CC'CwG_GG
Cuts at: 639 712 639
Size: 73 4435
PciI A'CATG_T
Cuts at: 2964 2964
Size: 4508
PflMI CCAn_nnn'nTGG
Cuts at: 508 508
Size: 4508
PmeI GTTT'AAAC
Cuts at: 1507 1507
Size: 4508
PmlI CAC'GTG
Cuts at: 879 879
Size: 4508
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1254 1286 3647 3679 1254
Size: 32 2361 32 2083
Fragments arranged by size:
2361 2083 32 32
PspXI vC'TCGA_Gb
Cuts at: 1435 1435
Size: 4508
PstI C_TGCA'G
Cuts at: 616 1085 1555 2240 616
Size: 469 470 685 2884
Fragments arranged by size:
2884 685 470 469
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4508
PvuII CAG'CTG
Cuts at: 508 1933 2293 508
Size: 1425 360 2723
Fragments arranged by size:
2723 1425 360
RsrII CG'GwC_CG
Cuts at: 2703 2703
Size: 4508
SacI G_AGCT'C
Cuts at: 1010 1522 1010
Size: 512 3996
SalI G'TCGA_C
Cuts at: 1545 1545
Size: 4508
SapI GCTCTTCn'nnn_
Cuts at: 1028 2531 2741 1028
Size: 1503 210 2795
Fragments arranged by size:
2795 1503 210
SbfI CC_TGCA'GG
Cuts at: 616 1555 616
Size: 939 3569
ScaI AGT'ACT
Cuts at: 4025 4025
Size: 4508
SfcI C'TryA_G
Cuts at: 33 612 1081 1278 1551 2236 3229 3420 33
Size: 579 469 197 273 685 993 191 1121
Fragments arranged by size:
1121 993 685 579 469 273 197 191
SfoI GGC'GCC
Cuts at: 1940 2188 1940
Size: 248 4260
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4508
SmaI CCC'GGG
Cuts at: 1530 1530
Size: 4508
SmlI C'TyrA_G
Cuts at: 184 1435 1663 2578 3070 3332 184
Size: 1251 228 915 492 262 1360
Fragments arranged by size:
1360 1251 915 492 262 228
SpeI A'CTAG_T
Cuts at: 67 718 67
Size: 651 3857
SphI G_CATG'C
Cuts at: 1404 1561 2061 1404
Size: 157 500 3851
Fragments arranged by size:
3851 500 157
StuI AGG'CCT
Cuts at: 1276 1276
Size: 4508
StyI C'CwwG_G
Cuts at: 138 1642 1996 138
Size: 1504 354 2650
Fragments arranged by size:
2650 1504 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2091 2224 2683 2892 2091
Size: 133 459 209 3707
Fragments arranged by size:
3707 459 209 133
TatI w'GTAC_w
Cuts at: 1040 4023 1040
Size: 2983 1525
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 440 472 672 704 4025 4057 440
Size: 32 200 32 3321 32 891
Fragments arranged by size:
3321 891 200 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2305 2305
Size: 4508
XbaI T'CTAG_A
Cuts at: 1539 1539
Size: 4508
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2003 2003
Size: 4508
XhoI C'TCGA_G
Cuts at: 1435 1435
Size: 4508
XmaI C'CCGG_G
Cuts at: 1528 1528
Size: 4508
ZraI GAC'GTC
Cuts at: 837 837
Size: 4508
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PmeI PmlI
PpiI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AleI ApaI AscI AvrII BaeI BbsI
BcgI BfrBI BmtI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PfoI
PpuMI PshAI PsiI PspOMI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XmnI