Restriction map for
pF1KB0408
(Circular) (Six-base) MAPSORT of: pF1KB0408.seq Check: 2475 from: 1 to: 5522
pF1KB0408 5522 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 312 2397 312
Size: 2085 3437
AccI GT'mk_AC
Cuts at: 1985 2560 1985
Size: 575 4947
Acc65I G'GTAC_C
Cuts at: 2538 2538
Size: 5522
AclI AA'CG_TT
Cuts at: 5362 5362
Size: 5522
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1204 1510 1522 1594 2212 3347 3779 4526 4794
Size: 306 12 72 618 1135 432 747 268
Cuts at: 4794 1204
Size: 1932
Fragments arranged by size:
1932 1135 747 618 432 306 268 72
12
AfeI AGC'GCT
Cuts at: 1792 1792
Size: 5522
AflIII A'CryG_T
Cuts at: 1613 2874 3978 4955 1613
Size: 1261 1104 977 2180
Fragments arranged by size:
2180 1261 1104 977
AgeI A'CCGG_T
Cuts at: 3910 3910
Size: 5522
AleI CACnn'nnGTG
Cuts at: 190 1682 1866 2146 190
Size: 1492 184 280 3566
Fragments arranged by size:
3566 1492 280 184
AlwNI CAG_nnn'CTG
Cuts at: 327 736 2337 2412 2494 2827 4394 327
Size: 409 1601 75 82 333 1567 1455
Fragments arranged by size:
1601 1567 1455 409 333 82 75
ApaI G_GGCC'C
Cuts at: 1526 1526
Size: 5522
ApaLI G'TGCA_C
Cuts at: 286 1339 2014 4292 286
Size: 1053 675 2278 1516
Fragments arranged by size:
2278 1516 1053 675
ApoI r'AATT_y
Cuts at: 2526 4875 5131 2526
Size: 2349 256 2917
Fragments arranged by size:
2917 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5522
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5522
AvaI C'yCGr_G
Cuts at: 674 895 933 959 1260 1311 2050 2542 674
Size: 221 38 26 301 51 739 492 3654
Fragments arranged by size:
3654 739 492 301 221 51 38 26
BamHI G'GATC_C
Cuts at: 1589 2547 1589
Size: 958 4564
BanI G'GyrC_C
Cuts at: 976 1580 2148 2538 2952 3200 3235 976
Size: 604 568 390 414 248 35 3263
Fragments arranged by size:
3263 604 568 414 390 248 35
BanII G_rGCy'C
Cuts at: 364 698 760 785 947 1526 2051 2536 3566
Size: 334 62 25 162 579 525 485 1030
Cuts at: 3566 364
Size: 2320
Fragments arranged by size:
2320 1030 579 525 485 334 162 62
25
BbeI G_GCGC'C
Cuts at: 2956 3204 2956
Size: 248 5274
BbsI GAAGACnn'nnnn_
Cuts at: 1174 2014 2240 2424 1174
Size: 840 226 184 4272
Fragments arranged by size:
4272 840 226 184
BbvCI CC'TCA_GC
Cuts at: 737 851 2453 737
Size: 114 1602 3806
Fragments arranged by size:
3806 1602 114
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1788 1822 1872 1906 1788
Size: 34 50 34 5404
Fragments arranged by size:
5404 50 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3420 4181 5522 3420
Size: 761 1341 3420
Fragments arranged by size:
3420 1341 761
BclI T'GATC_A
Cuts at: 3042 3042
Size: 5522
BglI GCCn_nnn'nGGC
Cuts at: 2403 2403
Size: 5522
BglII A'GATC_T
Cuts at: 3037 5074 3037
Size: 2037 3485
BlpI GC'TnA_GC
Cuts at: 352 452 547 2306 2634 352
Size: 100 95 1759 328 3240
Fragments arranged by size:
3240 1759 328 100 95
Bme1580I G_kGCm'C
Cuts at: 290 928 1343 1453 1526 2018 3147 3240 4296
Size: 638 415 110 73 492 1129 93 1056
Cuts at: 4296 290
Size: 1516
Fragments arranged by size:
1516 1129 1056 638 492 415 110 93
73
BmgBI CAC'GTC
Cuts at: 282 1668 282
Size: 1386 4136
BmrI ACTGGGnnnn_n'
Cuts at: 2734 2815 3150 5294 2734
Size: 81 335 2144 2962
Fragments arranged by size:
2962 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 966 3889 966
Size: 2923 2599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 577 898 1438 1955 2065 349
Size: 228 321 540 517 110 3806
Fragments arranged by size:
3806 540 517 321 228 110
Bpu10I CC'TnA_GC
Cuts at: 358 737 851 972 2453 358
Size: 379 114 121 1481 3427
Fragments arranged by size:
3427 1481 379 121 114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 586 682 1306 1513 2698 3577 4069 4367
Size: 162 96 624 207 1185 879 492 298
Cuts at: 4367 424
Size: 1579
Fragments arranged by size:
1579 1185 879 624 492 298 207 162
96
BsaI GGTCTCn'nnnn_
Cuts at: 1414 2470 5226 1414
Size: 1056 2756 1710
Fragments arranged by size:
2756 1710 1056
BsaAI yAC'GTr
Cuts at: 1614 1752 2004 2393 2875 1614
Size: 138 252 389 482 4261
Fragments arranged by size:
4261 482 389 252 138
BsaBI GATnn'nnATC
Cuts at: 6 1510 3036 5073 6
Size: 1504 1526 2037 455
Fragments arranged by size:
2037 1526 1504 455
BsaHI Gr'CG_yC
Cuts at: 2953 3201 3903 5399 2953
Size: 248 702 1496 3076
Fragments arranged by size:
3076 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2711 3232 3910 4184 4331 4742 2711
Size: 521 678 274 147 411 3491
Fragments arranged by size:
3491 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 322 352 1432 1462 1981 2011 2150 2180 322
Size: 30 1080 30 519 30 139 30 3664
Fragments arranged by size:
3664 1080 519 139 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 538 622 817 835 844 847 877 913 928
Size: 84 195 18 9 3 30 36 15
Cuts at: 928 1216 2068 538
Size: 288 852 3992
Fragments arranged by size:
3992 852 288 195 84 36 30 18
15 9 3
BseYI C'CCAG_C
Cuts at: 119 165 589 872 2419 2947 3705 4282 4778
Size: 46 424 283 1547 528 758 577 496
Cuts at: 4778 119
Size: 863
Fragments arranged by size:
1547 863 758 577 528 496 424 283
46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 665 1143 1228 1890 1899 2245 168
Size: 497 478 85 662 9 346 3445
Fragments arranged by size:
3445 662 497 478 346 85 9
BsiEI CG_ry'CG
Cuts at: 104 990 1148 1380 3064 3110 4318 104
Size: 886 158 232 1684 46 1208 1308
Fragments arranged by size:
1684 1308 1208 886 232 158 46
BsiHKAI G_wGCw'C
Cuts at: 290 698 760 947 1343 1445 2018 2536 3314
Size: 408 62 187 396 102 573 518 778
Cuts at: 3314 3504 4296 290
Size: 190 792 1516
Fragments arranged by size:
1516 792 778 573 518 408 396 190
187 102 62
BsmI GAATG_Cn'
Cuts at: 1673 1673
Size: 5522
BsmBI CGTCTCn'nnnn_
Cuts at: 2173 2173
Size: 5522
Bsp1286I G_dGCh'C
Cuts at: 290 364 698 760 785 928 947 1343 1445
Size: 74 334 62 25 143 19 396 102
Cuts at: 1445 1453 1526 2018 2051 2536 3147 3240 3314
Size: 8 73 492 33 485 611 93 74
Cuts at: 3314 3504 3566 4296 290
Size: 190 62 730 1516
Fragments arranged by size:
1516 730 611 492 485 396 334 190
143 102 93 74 74 73 62 62
33 25 19 8
BspEI T'CCGG_A
Cuts at: 4742 4742
Size: 5522
BspHI T'CATG_A
Cuts at: 4698 4698
Size: 5522
BspMI ACCTGCnnnn'nnnn_
Cuts at: 312 1250 2000 2397 2572 2786 3088 3469 312
Size: 938 750 397 175 214 302 381 2365
Fragments arranged by size:
2365 938 750 397 381 302 214 175
BsrBI CCG'CTC
Cuts at: 50 1382 3814 3868 5352 5517 50
Size: 1332 2432 54 1484 165 55
Fragments arranged by size:
2432 1484 1332 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3434 3434
Size: 5522
BsrFI r'CCGG_y
Cuts at: 456 2151 3520 3701 3910 456
Size: 1695 1369 181 209 2068
Fragments arranged by size:
2068 1695 1369 209 181
BsrGI T'GTAC_A
Cuts at: 338 338
Size: 5522
BssHII G'CGCG_C
Cuts at: 1270 1270
Size: 5522
BssSI C'ACGA_G
Cuts at: 711 1110 3793 4151 711
Size: 399 2683 358 2082
Fragments arranged by size:
2683 2082 399 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1625 1877 2412 2899 1625
Size: 252 535 487 4248
Fragments arranged by size:
4248 535 487 252
BstBI TT'CG_AA
Cuts at: 3883 3883
Size: 5522
BstXI CCAn_nnnn'nTGG
Cuts at: 1824 2301 1824
Size: 477 5045
BstYI r'GATC_y
Cuts at: 1 1505 1589 2039 2547 3014 3037 3372 3618
Size: 1504 84 450 508 467 23 335 246
Cuts at: 3618 4630 4716 4728 5074 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1504 1012 508 467 450 449 346 335
246 86 84 23 12
Bsu36I CC'TnA_GG
Cuts at: 1563 1563
Size: 5522
BtgI C'CryG_G
Cuts at: 107 389 107
Size: 282 5240
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1497 1728 3472 3653 3816 1497
Size: 231 1744 181 163 3203
Fragments arranged by size:
3203 1744 231 181 163
BtsI GCAGTG_nn'
Cuts at: 233 1455 1623 2067 2171 2765 233
Size: 1222 168 444 104 594 2990
Fragments arranged by size:
2990 1222 594 444 168 104
DraI TTT'AAA
Cuts at: 2521 2521
Size: 5522
DrdI GACnn_nn'nnGTC
Cuts at: 3228 4086 3228
Size: 858 4664
EaeI y'GGCC_r
Cuts at: 344 386 392 491 533 635 1145 1377 1826
Size: 42 6 99 42 102 510 232 449
Cuts at: 1826 3107 3281 3672 3699 5044 344
Size: 1281 174 391 27 1345 822
Fragments arranged by size:
1345 1281 822 510 449 391 232 174
102 99 42 42 27 6
EagI C'GGCC_G
Cuts at: 1145 1377 3107 1145
Size: 232 1730 3560
Fragments arranged by size:
3560 1730 232
EarI CTCTTCn'nnn_
Cuts at: 268 794 1207 1306 3545 3755 268
Size: 526 413 99 2239 210 2035
Fragments arranged by size:
2239 2035 526 413 210 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1846 2425 3730 4040 4186 5332 1846
Size: 579 1305 310 146 1146 2036
Fragments arranged by size:
2036 1305 1146 579 310 146
EcoICRI GAG'CTC
Cuts at: 696 758 945 2534 696
Size: 62 187 1589 3684
Fragments arranged by size:
3684 1589 187 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 577 898 1204 1438 1510 1522 1594 1955
Size: 228 321 306 234 72 12 72 361
Cuts at: 1955 2065 2212 3347 3779 4526 4794 349
Size: 110 147 1135 432 747 268 1077
Fragments arranged by size:
1135 1077 747 432 361 321 306 268
234 228 147 110 72 72 12
EcoNI CCTnn'n_nnAGG
Cuts at: 319 604 319
Size: 285 5237
EcoO109I rG'GnC_Cy
Cuts at: 586 597 891 963 1523 1905 2661 586
Size: 11 294 72 560 382 756 3447
Fragments arranged by size:
3447 756 560 382 294 72 11
EcoRI G'AATT_C
Cuts at: 2526 2526
Size: 5522
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 279 3541 279
Size: 3262 2260
FspI TGC'GCA
Cuts at: 3303 3303
Size: 5522
HaeII r_GCGC'y
Cuts at: 1329 1655 1794 2956 3204 4226 5205 1329
Size: 326 139 1162 248 1022 979 1646
Fragments arranged by size:
1646 1162 1022 979 326 248 139
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3364 3394 3396 3426 3364
Size: 30 2 30 5460
Fragments arranged by size:
5460 30 30 2
HincII GTy'rAC
Cuts at: 2561 2561
Size: 5522
KasI G'GCGC_C
Cuts at: 2952 3200 2952
Size: 248 5274
KpnI G_GTAC'C
Cuts at: 2542 2542
Size: 5522
MluI A'CGCG_T
Cuts at: 4955 4955
Size: 5522
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1415 4193 4377 1163
Size: 252 2778 184 2308
Fragments arranged by size:
2778 2308 252 184
MscI TGG'CCA
Cuts at: 394 493 535 1828 3283 394
Size: 99 42 1293 1455 2633
Fragments arranged by size:
2633 1455 1293 99 42
MslI CAynn'nnrTG
Cuts at: 190 1682 1698 1866 2146 3511 3638 190
Size: 1492 16 168 280 1365 127 2074
Fragments arranged by size:
2074 1492 1365 280 168 127 16
MspA1I CmG'CkG
Cuts at: 147 159 165 641 850 876 922 1280 1335
Size: 12 6 476 209 26 46 358 55
Cuts at: 1335 1346 1598 1662 2633 2947 3307 4320 4565
Size: 11 252 64 971 314 360 1013 245
Cuts at: 4565 147
Size: 1104
Fragments arranged by size:
1104 1013 971 476 360 358 314 252
245 209 64 55 46 26 12 11
6
NaeI GCC'GGC
Cuts at: 458 3703 458
Size: 3245 2277
NarI GG'CG_CC
Cuts at: 2953 3201 2953
Size: 248 5274
NcoI C'CATG_G
Cuts at: 107 107
Size: 5522
NgoMIV G'CCGG_C
Cuts at: 456 3701 456
Size: 3245 2277
NotI GC'GGCC_GC
Cuts at: 1377 1377
Size: 5522
NruI TCG'CGA
Cuts at: 2444 2444
Size: 5522
NspI r_CATG'y
Cuts at: 136 2314 2575 3075 3982 136
Size: 2178 261 500 907 1676
Fragments arranged by size:
2178 1676 907 500 261
PasI CC'CwG_GG
Cuts at: 469 469
Size: 5522
PciI A'CATG_T
Cuts at: 3978 3978
Size: 5522
PflMI CCAn_nnn'nTGG
Cuts at: 1335 2337 1335
Size: 1002 4520
PfoI T'CCnGG_A
Cuts at: 2042 2042
Size: 5522
PmeI GTTT'AAAC
Cuts at: 2521 2521
Size: 5522
PmlI CAC'GTG
Cuts at: 1752 2004 2393 1752
Size: 252 389 4881
Fragments arranged by size:
4881 389 252
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4661 4693 4661
Size: 32 5490
PpuMI rG'GwC_Cy
Cuts at: 597 1905 597
Size: 1308 4214
PshAI GACnn'nnGTC
Cuts at: 2128 2185 2128
Size: 57 5465
PspOMI G'GGCC_C
Cuts at: 1522 1522
Size: 5522
PstI C_TGCA'G
Cuts at: 244 732 1661 2166 2417 2569 3254 244
Size: 488 929 505 251 152 685 2512
Fragments arranged by size:
2512 929 685 505 488 251 152
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5522
PvuII CAG'CTG
Cuts at: 147 165 850 876 1335 2947 3307 147
Size: 18 685 26 459 1612 360 2362
Fragments arranged by size:
2362 1612 685 459 360 26 18
RsrII CG'GwC_CG
Cuts at: 3717 3717
Size: 5522
SacI G_AGCT'C
Cuts at: 698 760 947 2536 698
Size: 62 187 1589 3684
Fragments arranged by size:
3684 1589 187 62
SalI G'TCGA_C
Cuts at: 2559 2559
Size: 5522
SapI GCTCTTCn'nnn_
Cuts at: 268 1207 1306 3545 3755 268
Size: 939 99 2239 210 2035
Fragments arranged by size:
2239 2035 939 210 99
SbfI CC_TGCA'GG
Cuts at: 2569 2569
Size: 5522
ScaI AGT'ACT
Cuts at: 250 5039 250
Size: 4789 733
SexAI A'CCwGG_T
Cuts at: 1475 1475
Size: 5522
SfcI C'TryA_G
Cuts at: 33 240 728 1657 2162 2413 2486 2565 3250
Size: 207 488 929 505 251 73 79 685
Cuts at: 3250 4243 4434 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 929 685 505 488 251 207
191 79 73
SfoI GGC'GCC
Cuts at: 2954 3202 2954
Size: 248 5274
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5522
SmaI CCC'GGG
Cuts at: 676 961 2544 676
Size: 285 1583 3654
Fragments arranged by size:
3654 1583 285
SmlI C'TyrA_G
Cuts at: 439 601 697 1321 1528 2677 3592 4084 4346
Size: 162 96 624 207 1149 915 492 262
Cuts at: 4346 439
Size: 1615
Fragments arranged by size:
1615 1149 915 624 492 262 207 162
96
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5522
SphI G_CATG'C
Cuts at: 2575 3075 2575
Size: 500 5022
StuI AGG'CCT
Cuts at: 314 487 1006 1132 1720 1888 314
Size: 173 519 126 588 168 3948
Fragments arranged by size:
3948 588 519 173 168 126
StyI C'CwwG_G
Cuts at: 107 488 1001 2656 3010 107
Size: 381 513 1655 354 2619
Fragments arranged by size:
2619 1655 513 381 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 594 1741 3105 3238 3697 3906 594
Size: 1147 1364 133 459 209 2210
Fragments arranged by size:
2210 1364 1147 459 209 133
TatI w'GTAC_w
Cuts at: 248 338 5037 248
Size: 90 4699 733
Fragments arranged by size:
4699 733 90
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1432 1464 2150 2182 5039 5071 1432
Size: 32 686 32 2857 32 1883
Fragments arranged by size:
2857 1883 686 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2092 3319 2092
Size: 1227 4295
XbaI T'CTAG_A
Cuts at: 2553 2553
Size: 5522
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 717 2390 3017 717
Size: 1673 627 3222
Fragments arranged by size:
3222 1673 627
XmaI C'CCGG_G
Cuts at: 674 959 2542 674
Size: 285 1583 3654
Fragments arranged by size:
3654 1583 285
XmnI GAAnn'nnTTC
Cuts at: 1048 1105 1552 1048
Size: 57 447 5018
Fragments arranged by size:
5018 447 57
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NruI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BaeI
BfrBI BmtI BsiWI BstEII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NsiI PacI PsiI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI