Restriction map for
pF1KB0409
(Circular) (Six-base) MAPSORT of: pF1KB0409.seq Check: 317 from: 1 to: 4511
pF1KB0409 4511 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AccI GT'mk_AC
Cuts at: 1549 1549
Size: 4511
Acc65I G'GTAC_C
Cuts at: 1355 1527 1355
Size: 172 4339
AclI AA'CG_TT
Cuts at: 4351 4351
Size: 4511
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 2336 2768 3515 3783 136
Size: 2200 432 747 268 864
Fragments arranged by size:
2200 864 747 432 268
AfeI AGC'GCT
Cuts at: 616 616
Size: 4511
AflIII A'CryG_T
Cuts at: 293 1265 1863 2967 3944 293
Size: 972 598 1104 977 860
Fragments arranged by size:
1104 977 972 860 598
AgeI A'CCGG_T
Cuts at: 2899 2899
Size: 4511
AhdI GACnn_n'nnGTC
Cuts at: 660 660
Size: 4511
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1252 1284 1252
Size: 32 4479
AlwNI CAG_nnn'CTG
Cuts at: 792 1136 1149 1816 3383 792
Size: 344 13 667 1567 1920
Fragments arranged by size:
1920 1567 667 344 13
ApaLI G'TGCA_C
Cuts at: 3281 3281
Size: 4511
ApoI r'AATT_y
Cuts at: 1515 3864 4120 1515
Size: 2349 256 1906
Fragments arranged by size:
2349 1906 256
AscI GG'CGCG_CC
Cuts at: 285 285
Size: 4511
AseI AT'TA_AT
Cuts at: 20 20
Size: 4511
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4511
AvaI C'yCGr_G
Cuts at: 368 993 1531 368
Size: 625 538 3348
Fragments arranged by size:
3348 625 538
BamHI G'GATC_C
Cuts at: 1536 1536
Size: 4511
BanI G'GyrC_C
Cuts at: 278 607 626 997 1062 1159 1355 1527 1941
Size: 329 19 371 65 97 196 172 414
Cuts at: 1941 2189 2224 278
Size: 248 35 2565
Fragments arranged by size:
2565 414 371 329 248 196 172 97
65 35 19
BanII G_rGCy'C
Cuts at: 322 466 813 1178 1497 1525 2555 322
Size: 144 347 365 319 28 1030 2278
Fragments arranged by size:
2278 1030 365 347 319 144 28
BbeI G_GCGC'C
Cuts at: 282 1163 1945 2193 282
Size: 881 782 248 2600
Fragments arranged by size:
2600 881 782 248
BbsI GAAGACnn'nnnn_
Cuts at: 231 231
Size: 4511
BbvCI CC'TCA_GC
Cuts at: 925 925
Size: 4511
BciVI GTATCCnnnnn_n'
Cuts at: 2409 3170 4511 2409
Size: 761 1341 2409
Fragments arranged by size:
2409 1341 761
BclI T'GATC_A
Cuts at: 833 2031 833
Size: 1198 3313
BglII A'GATC_T
Cuts at: 374 2026 4063 374
Size: 1652 2037 822
Fragments arranged by size:
2037 1652 822
BlpI GC'TnA_GC
Cuts at: 1623 1623
Size: 4511
Bme1580I G_kGCm'C
Cuts at: 610 766 1000 1067 1196 1351 2136 2229 3285
Size: 156 234 67 129 155 785 93 1056
Cuts at: 3285 610
Size: 1836
Fragments arranged by size:
1836 1056 785 234 156 155 129 93
67
BmrI ACTGGGnnnn_n'
Cuts at: 1425 1723 1804 2139 4283 1425
Size: 298 81 335 2144 1653
Fragments arranged by size:
2144 1653 335 298 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 369 657 2878 369
Size: 288 2221 2002
Fragments arranged by size:
2221 2002 288
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 346 466 909 1192 322
Size: 24 120 443 283 3641
Fragments arranged by size:
3641 443 283 120 24
Bpu10I CC'TnA_GC
Cuts at: 182 925 182
Size: 743 3768
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 426 1687 2566 3058 3356 426
Size: 1261 879 492 298 1581
Fragments arranged by size:
1581 1261 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 117 858 1431 4215 117
Size: 741 573 2784 413
Fragments arranged by size:
2784 741 573 413
BsaAI yAC'GTr
Cuts at: 986 1352 1864 986
Size: 366 512 3633
Fragments arranged by size:
3633 512 366
BsaBI GATnn'nnATC
Cuts at: 6 2025 4062 6
Size: 2019 2037 455
Fragments arranged by size:
2037 2019 455
BsaHI Gr'CG_yC
Cuts at: 266 279 1160 1942 2190 2892 4388 266
Size: 13 881 782 248 702 1496 389
Fragments arranged by size:
1496 881 782 702 389 248 13
BsaWI w'CCGG_w
Cuts at: 1700 2221 2899 3173 3320 3731 1700
Size: 521 678 274 147 411 2480
Fragments arranged by size:
2480 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 319 334 349 364 1252 1282 319
Size: 15 15 15 888 30 3548
Fragments arranged by size:
3548 888 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 121 209 319 358 376 121
Size: 88 110 39 18 4256
Fragments arranged by size:
4256 110 88 39 18
BseYI C'CCAG_C
Cuts at: 909 979 1136 1376 1936 2694 3271 3767 909
Size: 70 157 240 560 758 577 496 1653
Fragments arranged by size:
1653 758 577 560 496 240 157 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 651 1015 256
Size: 395 364 3752
Fragments arranged by size:
3752 395 364
BsiEI CG_ry'CG
Cuts at: 104 316 2053 2099 3307 104
Size: 212 1737 46 1208 1308
Fragments arranged by size:
1737 1308 1208 212 46
BsiHKAI G_wGCw'C
Cuts at: 355 813 920 935 1178 1525 2303 2493 3285
Size: 458 107 15 243 347 778 190 792
Cuts at: 3285 355
Size: 1581
Fragments arranged by size:
1581 792 778 458 347 243 190 107
15
BsiWI C'GTAC_G
Cuts at: 986 986
Size: 4511
BsmBI CGTCTCn'nnnn_
Cuts at: 526 526
Size: 4511
Bsp1286I G_dGCh'C
Cuts at: 322 355 466 610 766 813 920 935 1000
Size: 33 111 144 156 47 107 15 65
Cuts at: 1000 1067 1178 1196 1351 1497 1525 2136 2229
Size: 67 111 18 155 146 28 611 93
Cuts at: 2229 2303 2493 2555 3285 322
Size: 74 190 62 730 1548
Fragments arranged by size:
1548 730 611 190 156 155 146 144
111 111 107 93 74 67 65 62
47 33 28 18 15
BspEI T'CCGG_A
Cuts at: 3731 3731
Size: 4511
BspHI T'CATG_A
Cuts at: 3687 3687
Size: 4511
BspMI ACCTGCnnnn'nnnn_
Cuts at: 777 957 1561 1775 2077 2458 777
Size: 180 604 214 302 381 2830
Fragments arranged by size:
2830 604 381 302 214 180
BsrBI CCG'CTC
Cuts at: 50 2803 2857 4341 4506 50
Size: 2753 54 1484 165 55
Fragments arranged by size:
2753 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2423 2423
Size: 4511
BsrFI r'CCGG_y
Cuts at: 275 281 953 1276 2509 2690 2899 275
Size: 6 672 323 1233 181 209 1887
Fragments arranged by size:
1887 1233 672 323 209 181 6
BsrGI T'GTAC_A
Cuts at: 1415 1415
Size: 4511
BssHII G'CGCG_C
Cuts at: 285 285
Size: 4511
BssSI C'ACGA_G
Cuts at: 631 2782 3140 631
Size: 2151 358 2002
Fragments arranged by size:
2151 2002 358
BstAPI GCAn_nnn'nTGC
Cuts at: 761 1888 761
Size: 1127 3384
BstBI TT'CG_AA
Cuts at: 2872 2872
Size: 4511
BstXI CCAn_nnnn'nTGG
Cuts at: 849 1170 849
Size: 321 4190
BstYI r'GATC_y
Cuts at: 1 374 1536 2003 2026 2361 2607 3619 3705
Size: 373 1162 467 23 335 246 1012 86
Cuts at: 3705 3717 4063 1
Size: 12 346 449
Fragments arranged by size:
1162 1012 467 449 373 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 124 189 124
Size: 65 4446
BtgI C'CryG_G
Cuts at: 107 214 261 781 857 1040 1155 1163 1397
Size: 107 47 520 76 183 115 8 234
Cuts at: 1397 107
Size: 3221
Fragments arranged by size:
3221 520 234 183 115 107 76 47
8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 999 2461 2642 2805 999
Size: 1462 181 163 2705
Fragments arranged by size:
2705 1462 181 163
BtsI GCAGTG_nn'
Cuts at: 355 600 1754 355
Size: 245 1154 3112
Fragments arranged by size:
3112 1154 245
DraI TTT'AAA
Cuts at: 1510 1510
Size: 4511
DrdI GACnn_nn'nnGTC
Cuts at: 2217 3075 2217
Size: 858 3653
EaeI y'GGCC_r
Cuts at: 313 2096 2270 2661 2688 4033 313
Size: 1783 174 391 27 1345 791
Fragments arranged by size:
1783 1345 791 391 174 27
EagI C'GGCC_G
Cuts at: 313 2096 313
Size: 1783 2728
EarI CTCTTCn'nnn_
Cuts at: 2534 2744 2534
Size: 210 4301
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1015 2719 3029 3175 4321 1015
Size: 1704 310 146 1146 1205
Fragments arranged by size:
1704 1205 1146 310 146
EcoICRI GAG'CTC
Cuts at: 811 1176 1523 811
Size: 365 347 3799
Fragments arranged by size:
3799 365 347
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 322 346 466 909 1192 2336 2768 3515
Size: 186 24 120 443 283 1144 432 747
Cuts at: 3515 3783 136
Size: 268 864
Fragments arranged by size:
1144 864 747 443 432 283 268 186
120 24
EcoO109I rG'GnC_Cy
Cuts at: 120 226 789 827 1650 120
Size: 106 563 38 823 2981
Fragments arranged by size:
2981 823 563 106 38
EcoRI G'AATT_C
Cuts at: 1515 1515
Size: 4511
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2530 2530
Size: 4511
FspI TGC'GCA
Cuts at: 1392 2292 1392
Size: 900 3611
HaeII r_GCGC'y
Cuts at: 282 618 1163 1945 2193 3215 4194 282
Size: 336 545 782 248 1022 979 599
Fragments arranged by size:
1022 979 782 599 545 336 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 646 678 2353 2383 2385 2415 646
Size: 32 1675 30 2 30 2742
Fragments arranged by size:
2742 1675 32 30 30 2
HincII GTy'rAC
Cuts at: 1550 1550
Size: 4511
KasI G'GCGC_C
Cuts at: 278 1159 1941 2189 278
Size: 881 782 248 2600
Fragments arranged by size:
2600 881 782 248
KpnI G_GTAC'C
Cuts at: 1359 1531 1359
Size: 172 4339
MluI A'CGCG_T
Cuts at: 293 3944 293
Size: 3651 860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3182 3366 3182
Size: 184 4327
MscI TGG'CCA
Cuts at: 2272 2272
Size: 4511
MslI CAynn'nnrTG
Cuts at: 542 847 2500 2627 542
Size: 305 1653 127 2426
Fragments arranged by size:
2426 1653 305 127
MspA1I CmG'CkG
Cuts at: 552 859 1133 1146 1376 1622 1936 2296 3309
Size: 307 274 13 230 246 314 360 1013
Cuts at: 3309 3554 552
Size: 245 1509
Fragments arranged by size:
1509 1013 360 314 307 274 246 245
230 13
NaeI GCC'GGC
Cuts at: 283 955 1278 2692 283
Size: 672 323 1414 2102
Fragments arranged by size:
2102 1414 672 323
NarI GG'CG_CC
Cuts at: 279 1160 1942 2190 279
Size: 881 782 248 2600
Fragments arranged by size:
2600 881 782 248
NcoI C'CATG_G
Cuts at: 107 781 107
Size: 674 3837
NgoMIV G'CCGG_C
Cuts at: 281 953 1276 2690 281
Size: 672 323 1414 2102
Fragments arranged by size:
2102 1414 672 323
NspI r_CATG'y
Cuts at: 213 1269 1333 1564 2064 2971 213
Size: 1056 64 231 500 907 1753
Fragments arranged by size:
1753 1056 907 500 231 64
PciI A'CATG_T
Cuts at: 1265 2967 1265
Size: 1702 2809
PflMI CCAn_nnn'nTGG
Cuts at: 1041 1149 1041
Size: 108 4403
PfoI T'CCnGG_A
Cuts at: 299 299
Size: 4511
PmeI GTTT'AAAC
Cuts at: 1510 1510
Size: 4511
PmlI CAC'GTG
Cuts at: 1352 1352
Size: 4511
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1252 1284 3650 3682 1252
Size: 32 2366 32 2081
Fragments arranged by size:
2366 2081 32 32
PpuMI rG'GwC_Cy
Cuts at: 789 827 789
Size: 38 4473
PstI C_TGCA'G
Cuts at: 171 595 1223 1558 2243 171
Size: 424 628 335 685 2439
Fragments arranged by size:
2439 685 628 424 335
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4511
PvuII CAG'CTG
Cuts at: 552 1146 1376 1936 2296 552
Size: 594 230 560 360 2767
Fragments arranged by size:
2767 594 560 360 230
RsrII CG'GwC_CG
Cuts at: 2706 2706
Size: 4511
SacI G_AGCT'C
Cuts at: 813 1178 1525 813
Size: 365 347 3799
Fragments arranged by size:
3799 365 347
SacII CC_GC'GG
Cuts at: 860 860
Size: 4511
SalI G'TCGA_C
Cuts at: 1548 1548
Size: 4511
SanDI GG'GwC_CC
Cuts at: 827 827
Size: 4511
SapI GCTCTTCn'nnn_
Cuts at: 2534 2744 2534
Size: 210 4301
SbfI CC_TGCA'GG
Cuts at: 1558 1558
Size: 4511
ScaI AGT'ACT
Cuts at: 589 4028 589
Size: 3439 1072
SfcI C'TryA_G
Cuts at: 33 167 591 1219 1554 2239 3232 3423 33
Size: 134 424 628 335 685 993 191 1121
Fragments arranged by size:
1121 993 685 628 424 335 191 134
SfoI GGC'GCC
Cuts at: 280 1161 1943 2191 280
Size: 881 782 248 2600
Fragments arranged by size:
2600 881 782 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4511
SgrAI Cr'CCGG_yG
Cuts at: 275 281 275
Size: 6 4505
SmaI CCC'GGG
Cuts at: 370 995 1533 370
Size: 625 538 3348
Fragments arranged by size:
3348 625 538
SmlI C'TyrA_G
Cuts at: 441 1666 2581 3073 3335 441
Size: 1225 915 492 262 1617
Fragments arranged by size:
1617 1225 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4511
SphI G_CATG'C
Cuts at: 1564 2064 1564
Size: 500 4011
StuI AGG'CCT
Cuts at: 187 187
Size: 4511
StyI C'CwwG_G
Cuts at: 107 735 781 1645 1999 107
Size: 628 46 864 354 2619
Fragments arranged by size:
2619 864 628 354 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 687 837 1351 2094 2227 2686 2895 687
Size: 150 514 743 133 459 209 2303
Fragments arranged by size:
2303 743 514 459 209 150 133
TatI w'GTAC_w
Cuts at: 587 1262 1415 1433 4026 587
Size: 675 153 18 2593 1072
Fragments arranged by size:
2593 1072 675 153 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 199 231 319 334 351 366 4028 4060 199
Size: 32 88 15 17 15 3662 32 650
Fragments arranged by size:
3662 650 88 32 32 17 15 15
Tth111I GACn'n_nGTC
Cuts at: 2308 2308
Size: 4511
XbaI T'CTAG_A
Cuts at: 708 1542 708
Size: 834 3677
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 297 2006 297
Size: 1709 2802
XmaI C'CCGG_G
Cuts at: 368 993 1531 368
Size: 625 538 3348
Fragments arranged by size:
3348 625 538
XmnI GAAnn'nnTTC
Cuts at: 403 403
Size: 4511
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI ApaI AvrII BaeI
BcgI BfrBI BglI BmgBI BmtI BsmI BstEII BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PshAI PsiI PspOMI PspXI PsrI SexAI SfiI
SnaBI SrfI SspI SwaI XhoI ZraI