Restriction map for
pF1KB0410
(Circular) (Six-base) MAPSORT of: pF1KB0410.seq Check: 8239 from: 1 to: 5579
pF1KB0410 5579 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 632 632
Size: 5579
AccI GT'mk_AC
Cuts at: 2617 2617
Size: 5579
Acc65I G'GTAC_C
Cuts at: 2595 2595
Size: 5579
AclI AA'CG_TT
Cuts at: 5419 5419
Size: 5579
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1676 1868 3404 3836 4583 4851 1676
Size: 192 1536 432 747 268 2404
Fragments arranged by size:
2404 1536 747 432 268 192
AfeI AGC'GCT
Cuts at: 240 240
Size: 5579
AflIII A'CryG_T
Cuts at: 2931 4035 5012 2931
Size: 1104 977 3498
Fragments arranged by size:
3498 1104 977
AgeI A'CCGG_T
Cuts at: 3967 3967
Size: 5579
AleI CACnn'nnGTG
Cuts at: 430 430
Size: 5579
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 385 448 385
Size: 63 5516
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 541 573 541
Size: 32 5547
AlwNI CAG_nnn'CTG
Cuts at: 411 1683 2884 4451 411
Size: 1272 1201 1567 1539
Fragments arranged by size:
1567 1539 1272 1201
ApaI G_GGCC'C
Cuts at: 140 236 140
Size: 96 5483
ApaLI G'TGCA_C
Cuts at: 391 1219 4349 391
Size: 828 3130 1621
Fragments arranged by size:
3130 1621 828
ApoI r'AATT_y
Cuts at: 502 1130 2583 4932 5188 502
Size: 628 1453 2349 256 893
Fragments arranged by size:
2349 1453 893 628 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5579
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5579
AvaI C'yCGr_G
Cuts at: 128 228 399 805 2599 128
Size: 100 171 406 1794 3108
Fragments arranged by size:
3108 1794 406 171 100
BamHI G'GATC_C
Cuts at: 2604 2604
Size: 5579
BanI G'GyrC_C
Cuts at: 432 644 2595 3009 3257 3292 432
Size: 212 1951 414 248 35 2719
Fragments arranged by size:
2719 1951 414 248 212 35
BanII G_rGCy'C
Cuts at: 140 236 380 1558 2051 2593 3623 140
Size: 96 144 1178 493 542 1030 2096
Fragments arranged by size:
2096 1178 1030 542 493 144 96
BbeI G_GCGC'C
Cuts at: 3013 3261 3013
Size: 248 5331
BbsI GAAGACnn'nnnn_
Cuts at: 1306 2562 1306
Size: 1256 4323
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 136 170 1063 1097 136
Size: 34 893 34 4618
Fragments arranged by size:
4618 893 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3477 4238 5579 3477
Size: 761 1341 3477
Fragments arranged by size:
3477 1341 761
BclI T'GATC_A
Cuts at: 1598 3099 1598
Size: 1501 4078
BglI GCCn_nnn'nGGC
Cuts at: 124 124
Size: 5579
BglII A'GATC_T
Cuts at: 737 2363 3094 5131 737
Size: 1626 731 2037 1185
Fragments arranged by size:
2037 1626 1185 731
BlpI GC'TnA_GC
Cuts at: 1676 2691 1676
Size: 1015 4564
Bme1580I G_kGCm'C
Cuts at: 140 236 395 437 1223 3204 3297 4353 140
Size: 96 159 42 786 1981 93 1056 1366
Fragments arranged by size:
1981 1366 1056 786 159 96 93 42
BmgBI CAC'GTC
Cuts at: 390 390
Size: 5579
BmrI ACTGGGnnnn_n'
Cuts at: 844 1040 2791 2872 3207 5351 844
Size: 196 1751 81 335 2144 1072
Fragments arranged by size:
2144 1751 1072 335 196 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3946 3946
Size: 5579
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 1416 2096 2105 2489 394
Size: 1022 680 9 384 3484
Fragments arranged by size:
3484 1022 680 384 9
Bpu10I CC'TnA_GC
Cuts at: 2339 2339
Size: 5579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2755 3634 4126 4424 2755
Size: 879 492 298 3910
Fragments arranged by size:
3910 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 620 754 5283 620
Size: 134 4529 916
Fragments arranged by size:
4529 916 134
BsaAI yAC'GTr
Cuts at: 2932 2932
Size: 5579
BsaBI GATnn'nnATC
Cuts at: 6 3093 5130 6
Size: 3087 2037 455
Fragments arranged by size:
3087 2037 455
BsaHI Gr'CG_yC
Cuts at: 3010 3258 3960 5456 3010
Size: 248 702 1496 3133
Fragments arranged by size:
3133 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2420 2768 3289 3967 4241 4388 4799 2420
Size: 348 521 678 274 147 411 3200
Fragments arranged by size:
3200 678 521 411 348 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 184 187 448 843 2037 2059 184
Size: 3 261 395 1194 22 3704
Fragments arranged by size:
3704 1194 395 261 22 3
BseYI C'CCAG_C
Cuts at: 235 884 3004 3762 4339 4835 235
Size: 649 2120 758 577 496 979
Fragments arranged by size:
2120 979 758 649 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2182 2270 2182
Size: 88 5491
BsiEI CG_ry'CG
Cuts at: 104 119 278 3121 3167 4375 104
Size: 15 159 2843 46 1208 1308
Fragments arranged by size:
2843 1308 1208 159 46 15
BsiHKAI G_wGCw'C
Cuts at: 380 395 932 1076 1223 2345 2593 3371 3561
Size: 15 537 144 147 1122 248 778 190
Cuts at: 3561 4353 380
Size: 792 1606
Fragments arranged by size:
1606 1122 792 778 537 248 190 147
144 15
BsmI GAATG_Cn'
Cuts at: 244 913 244
Size: 669 4910
Bsp1286I G_dGCh'C
Cuts at: 140 236 380 395 437 932 1076 1223 1558
Size: 96 144 15 42 495 144 147 335
Cuts at: 1558 2051 2345 2593 3204 3297 3371 3561 3623
Size: 493 294 248 611 93 74 190 62
Cuts at: 3623 4353 140
Size: 730 1366
Fragments arranged by size:
1366 730 611 495 493 335 294 248
190 147 144 144 96 93 74 62
42 15
BspEI T'CCGG_A
Cuts at: 4799 4799
Size: 5579
BspHI T'CATG_A
Cuts at: 4755 4755
Size: 5579
BspMI ACCTGCnnnn'nnnn_
Cuts at: 632 879 1704 2629 2843 3145 3526 632
Size: 247 825 925 214 302 381 2685
Fragments arranged by size:
2685 925 825 381 302 247 214
BsrBI CCG'CTC
Cuts at: 50 302 444 3871 3925 5409 5574 50
Size: 252 142 3427 54 1484 165 55
Fragments arranged by size:
3427 1484 252 165 142 55 54
BsrDI GCAATG_nn'
Cuts at: 1959 2486 3491 1959
Size: 527 1005 4047
Fragments arranged by size:
4047 1005 527
BsrFI r'CCGG_y
Cuts at: 273 3577 3758 3967 273
Size: 3304 181 209 1885
Fragments arranged by size:
3304 1885 209 181
BssSI C'ACGA_G
Cuts at: 3850 4208 3850
Size: 358 5221
BstAPI GCAn_nnn'nTGC
Cuts at: 2013 2956 2013
Size: 943 4636
BstBI TT'CG_AA
Cuts at: 3940 3940
Size: 5579
BstXI CCAn_nnnn'nTGG
Cuts at: 1998 1998
Size: 5579
BstYI r'GATC_y
Cuts at: 1 737 2363 2511 2604 3071 3094 3429 3675
Size: 736 1626 148 93 467 23 335 246
Cuts at: 3675 4687 4773 4785 5131 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1626 1012 736 467 449 346 335 246
148 93 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 566 1849 566
Size: 1283 4296
BtgI C'CryG_G
Cuts at: 140 350 524 620 665 2235 140
Size: 210 174 96 45 1570 3484
Fragments arranged by size:
3484 1570 210 174 96 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 348 644 3529 3710 3873 348
Size: 296 2885 181 163 2054
Fragments arranged by size:
2885 2054 296 181 163
BtsI GCAGTG_nn'
Cuts at: 715 930 1375 1556 1815 2822 715
Size: 215 445 181 259 1007 3472
Fragments arranged by size:
3472 1007 445 259 215 181
DraI TTT'AAA
Cuts at: 1751 2578 1751
Size: 827 4752
DraIII CAC_nnn'GTG
Cuts at: 339 339
Size: 5579
DrdI GACnn_nn'nnGTC
Cuts at: 526 3285 4143 526
Size: 2759 858 1962
Fragments arranged by size:
2759 1962 858
EaeI y'GGCC_r
Cuts at: 116 275 1574 2406 3164 3338 3729 3756 5101
Size: 159 1299 832 758 174 391 27 1345
Cuts at: 5101 116
Size: 594
Fragments arranged by size:
1345 1299 832 758 594 391 174 159
27
EagI C'GGCC_G
Cuts at: 116 275 3164 116
Size: 159 2889 2531
Fragments arranged by size:
2889 2531 159
EarI CTCTTCn'nnn_
Cuts at: 1014 2099 2261 3602 3812 1014
Size: 1085 162 1341 210 2781
Fragments arranged by size:
2781 1341 1085 210 162
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 238 3787 4097 4243 5389 238
Size: 3549 310 146 1146 428
Fragments arranged by size:
3549 1146 428 310 146
EcoICRI GAG'CTC
Cuts at: 378 2591 378
Size: 2213 3366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 1416 1676 1868 2096 2105 2489 3404 3836
Size: 1022 260 192 228 9 384 915 432
Cuts at: 3836 4583 4851 394
Size: 747 268 1122
Fragments arranged by size:
1122 1022 915 747 432 384 268 260
228 192 9
EcoO109I rG'GnC_Cy
Cuts at: 136 232 408 519 789 2718 136
Size: 96 176 111 270 1929 2997
Fragments arranged by size:
2997 1929 270 176 111 96
EcoRI G'AATT_C
Cuts at: 2583 2583
Size: 5579
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1313 2196 3598 1313
Size: 883 1402 3294
Fragments arranged by size:
3294 1402 883
FspI TGC'GCA
Cuts at: 3360 3360
Size: 5579
HaeII r_GCGC'y
Cuts at: 242 3013 3261 4283 5262 242
Size: 2771 248 1022 979 559
Fragments arranged by size:
2771 1022 979 559 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1845 1877 3421 3451 3453 3483 1845
Size: 32 1544 30 2 30 3941
Fragments arranged by size:
3941 1544 32 30 30 2
HincII GTy'rAC
Cuts at: 726 1620 2618 726
Size: 894 998 3687
Fragments arranged by size:
3687 998 894
HindIII A'AGCT_T
Cuts at: 1784 1837 1784
Size: 53 5526
HpaI GTT'AAC
Cuts at: 1620 1620
Size: 5579
KasI G'GCGC_C
Cuts at: 3009 3257 3009
Size: 248 5331
KpnI G_GTAC'C
Cuts at: 2599 2599
Size: 5579
MfeI C'AATT_G
Cuts at: 1022 1382 1022
Size: 360 5219
MluI A'CGCG_T
Cuts at: 5012 5012
Size: 5579
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 527 4250 4434 527
Size: 3723 184 1672
Fragments arranged by size:
3723 1672 184
MscI TGG'CCA
Cuts at: 1576 2408 3340 1576
Size: 832 932 3815
Fragments arranged by size:
3815 932 832
MslI CAynn'nnrTG
Cuts at: 367 430 3568 3695 367
Size: 63 3138 127 2251
Fragments arranged by size:
3138 2251 127 63
MspA1I CmG'CkG
Cuts at: 142 217 315 452 611 720 981 1455 1672
Size: 75 98 137 159 109 261 474 217
Cuts at: 1672 2237 2690 3004 3364 4377 4622 142
Size: 565 453 314 360 1013 245 1099
Fragments arranged by size:
1099 1013 565 474 453 360 314 261
245 217 159 137 109 98 75
NaeI GCC'GGC
Cuts at: 275 3760 275
Size: 3485 2094
NarI GG'CG_CC
Cuts at: 3010 3258 3010
Size: 248 5331
NcoI C'CATG_G
Cuts at: 620 665 620
Size: 45 5534
NgoMIV G'CCGG_C
Cuts at: 273 3758 273
Size: 3485 2094
NspI r_CATG'y
Cuts at: 843 2632 3132 4039 843
Size: 1789 500 907 2383
Fragments arranged by size:
2383 1789 907 500
PciI A'CATG_T
Cuts at: 4035 4035
Size: 5579
PflMI CCAn_nnn'nTGG
Cuts at: 411 411
Size: 5579
PfoI T'CCnGG_A
Cuts at: 1682 2114 1682
Size: 432 5147
PmeI GTTT'AAAC
Cuts at: 2578 2578
Size: 5579
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4718 4750 4718
Size: 32 5547
PpuMI rG'GwC_Cy
Cuts at: 408 519 408
Size: 111 5468
PshAI GACnn'nnGTC
Cuts at: 850 850
Size: 5579
PspOMI G'GGCC_C
Cuts at: 136 232 136
Size: 96 5483
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1407 1439 1407
Size: 32 5547
PstI C_TGCA'G
Cuts at: 290 710 1401 2018 2626 3311 290
Size: 420 691 617 608 685 2558
Fragments arranged by size:
2558 691 685 617 608 420
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5579
PvuII CAG'CTG
Cuts at: 452 611 720 981 1455 1672 3004 3364 452
Size: 159 109 261 474 217 1332 360 2667
Fragments arranged by size:
2667 1332 474 360 261 217 159 109
RsrII CG'GwC_CG
Cuts at: 3774 3774
Size: 5579
SacI G_AGCT'C
Cuts at: 380 2593 380
Size: 2213 3366
SacII CC_GC'GG
Cuts at: 143 2238 143
Size: 2095 3484
SalI G'TCGA_C
Cuts at: 2616 2616
Size: 5579
SapI GCTCTTCn'nnn_
Cuts at: 3602 3812 3602
Size: 210 5369
SbfI CC_TGCA'GG
Cuts at: 2018 2626 2018
Size: 608 4971
ScaI AGT'ACT
Cuts at: 5096 5096
Size: 5579
SexAI A'CCwGG_T
Cuts at: 410 1631 410
Size: 1221 4358
SfcI C'TryA_G
Cuts at: 33 286 706 1397 2014 2346 2622 3307 4300
Size: 253 420 691 617 332 276 685 993
Cuts at: 4300 4491 33
Size: 191 1121
Fragments arranged by size:
1121 993 691 685 617 420 332 276
253 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 124 124
Size: 5579
SfoI GGC'GCC
Cuts at: 3011 3259 3011
Size: 248 5331
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5579
SmaI CCC'GGG
Cuts at: 130 230 401 2601 130
Size: 100 171 2200 3108
Fragments arranged by size:
3108 2200 171 100
SmlI C'TyrA_G
Cuts at: 2734 3649 4141 4403 2734
Size: 915 492 262 3910
Fragments arranged by size:
3910 915 492 262
SpeI A'CTAG_T
Cuts at: 67 1929 67
Size: 1862 3717
SphI G_CATG'C
Cuts at: 843 2632 3132 843
Size: 1789 500 3290
Fragments arranged by size:
3290 1789 500
StuI AGG'CCT
Cuts at: 1367 2012 1367
Size: 645 4934
StyI C'CwwG_G
Cuts at: 515 620 665 2124 2713 3067 515
Size: 105 45 1459 589 354 3027
Fragments arranged by size:
3027 1459 589 354 105 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 898 1921 3162 3295 3754 3963 898
Size: 1023 1241 133 459 209 2514
Fragments arranged by size:
2514 1241 1023 459 209 133
TatI w'GTAC_w
Cuts at: 602 5094 602
Size: 4492 1087
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5096 5128 5096
Size: 32 5547
Tth111I GACn'n_nGTC
Cuts at: 3376 3376
Size: 5579
XbaI T'CTAG_A
Cuts at: 2610 2610
Size: 5579
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3074 3074
Size: 5579
XmaI C'CCGG_G
Cuts at: 128 228 399 2599 128
Size: 100 171 2200 3108
Fragments arranged by size:
3108 2200 171 100
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AscI AvrII BaeI BbvCI BfrBI
BmtI BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
ClaI CspCI EcoNI EcoRV FseI FspAI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PsiI PspXI
SanDI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI