Restriction map for
pF1KB0411
(Circular) (Six-base) MAPSORT of: pF1KB0411.seq Check: 5955 from: 1 to: 4514
pF1KB0411 4514 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AccI GT'mk_AC
Cuts at: 563 1552 563
Size: 989 3525
Acc65I G'GTAC_C
Cuts at: 315 1530 315
Size: 1215 3299
AclI AA'CG_TT
Cuts at: 4354 4354
Size: 4514
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2339 2771 3518 3786 2339
Size: 432 747 268 3067
Fragments arranged by size:
3067 747 432 268
AfeI AGC'GCT
Cuts at: 1096 1096
Size: 4514
AflIII A'CryG_T
Cuts at: 1866 2970 3947 1866
Size: 1104 977 2433
Fragments arranged by size:
2433 1104 977
AgeI A'CCGG_T
Cuts at: 2902 2902
Size: 4514
AhdI GACnn_n'nnGTC
Cuts at: 254 254
Size: 4514
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1191 1354 1191
Size: 163 4351
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 510 542 734 766 510
Size: 32 192 32 4258
Fragments arranged by size:
4258 192 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1308 1819 3386 1308
Size: 511 1567 2436
Fragments arranged by size:
2436 1567 511
ApaLI G'TGCA_C
Cuts at: 1389 3284 1389
Size: 1895 2619
ApoI r'AATT_y
Cuts at: 428 787 1518 3867 4123 428
Size: 359 731 2349 256 819
Fragments arranged by size:
2349 819 731 359 256
AseI AT'TA_AT
Cuts at: 20 157 20
Size: 137 4377
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4514
AvaI C'yCGr_G
Cuts at: 542 1534 542
Size: 992 3522
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 307 340 307
Size: 33 4481
BamHI G'GATC_C
Cuts at: 1539 1539
Size: 4514
BanI G'GyrC_C
Cuts at: 315 547 1075 1530 1944 2192 2227 315
Size: 232 528 455 414 248 35 2602
Fragments arranged by size:
2602 528 455 414 248 232 35
BanII G_rGCy'C
Cuts at: 994 1528 2558 994
Size: 534 1030 2950
Fragments arranged by size:
2950 1030 534
BbeI G_GCGC'C
Cuts at: 551 1948 2196 551
Size: 1397 248 2869
Fragments arranged by size:
2869 1397 248
BbsI GAAGACnn'nnnn_
Cuts at: 1166 1166
Size: 4514
BciVI GTATCCnnnnn_n'
Cuts at: 2412 3173 4514 2412
Size: 761 1341 2412
Fragments arranged by size:
2412 1341 761
BclI T'GATC_A
Cuts at: 2034 2034
Size: 4514
BglII A'GATC_T
Cuts at: 2029 4066 2029
Size: 2037 2477
BlpI GC'TnA_GC
Cuts at: 1107 1626 1107
Size: 519 3995
Bme1580I G_kGCm'C
Cuts at: 1078 1393 2139 2232 3288 1078
Size: 315 746 93 1056 2304
Fragments arranged by size:
2304 1056 746 315 93
BmrI ACTGGGnnnn_n'
Cuts at: 233 610 1726 1807 2142 4286 233
Size: 377 1116 81 335 2144 461
Fragments arranged by size:
2144 1116 461 377 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2881 2881
Size: 4514
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 775 784 1234 775
Size: 9 450 4055
Fragments arranged by size:
4055 450 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 1405 1690 2569 3061 3359 805
Size: 600 285 879 492 298 1960
Fragments arranged by size:
1960 879 600 492 298 285
BsaI GGTCTCn'nnnn_
Cuts at: 4218 4218
Size: 4514
BsaAI yAC'GTr
Cuts at: 1867 1867
Size: 4514
BsaBI GATnn'nnATC
Cuts at: 6 2028 4065 6
Size: 2022 2037 455
Fragments arranged by size:
2037 2022 455
BsaHI Gr'CG_yC
Cuts at: 548 1945 2193 2895 4391 548
Size: 1397 248 702 1496 671
Fragments arranged by size:
1496 1397 702 671 248
BsaWI w'CCGG_w
Cuts at: 1703 2224 2902 3176 3323 3734 1703
Size: 521 678 274 147 411 2483
Fragments arranged by size:
2483 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 734 764 734
Size: 30 4484
BseYI C'CCAG_C
Cuts at: 1939 2697 3274 3770 1939
Size: 758 577 496 2683
Fragments arranged by size:
2683 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1029 1029
Size: 4514
BsiEI CG_ry'CG
Cuts at: 104 2056 2102 3310 104
Size: 1952 46 1208 1308
Fragments arranged by size:
1952 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 124 1393 1528 2306 2496 3288 124
Size: 1269 135 778 190 792 1350
Fragments arranged by size:
1350 1269 792 778 190 135
Bsp1286I G_dGCh'C
Cuts at: 124 994 1078 1393 1528 2139 2232 2306 2496
Size: 870 84 315 135 611 93 74 190
Cuts at: 2496 2558 3288 124
Size: 62 730 1350
Fragments arranged by size:
1350 870 730 611 315 190 135 93
84 74 62
BspEI T'CCGG_A
Cuts at: 3734 3734
Size: 4514
BspHI T'CATG_A
Cuts at: 861 3690 861
Size: 2829 1685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1564 1778 2080 2461 1564
Size: 214 302 381 3617
Fragments arranged by size:
3617 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2806 2860 4344 4509 50
Size: 2756 54 1484 165 55
Fragments arranged by size:
2756 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1163 1373 2426 1163
Size: 210 1053 3251
Fragments arranged by size:
3251 1053 210
BsrFI r'CCGG_y
Cuts at: 2512 2693 2902 2512
Size: 181 209 4124
Fragments arranged by size:
4124 209 181
BsrGI T'GTAC_A
Cuts at: 1127 1127
Size: 4514
BssSI C'ACGA_G
Cuts at: 667 2785 3143 667
Size: 2118 358 2038
Fragments arranged by size:
2118 2038 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1891 1891
Size: 4514
BstBI TT'CG_AA
Cuts at: 2875 2875
Size: 4514
BstYI r'GATC_y
Cuts at: 1 1539 2006 2029 2364 2610 3622 3708 3720
Size: 1538 467 23 335 246 1012 86 12
Cuts at: 3720 4066 1
Size: 346 449
Fragments arranged by size:
1538 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 564 564
Size: 4514
Bsu36I CC'TnA_GG
Cuts at: 959 959
Size: 4514
BtgI C'CryG_G
Cuts at: 107 319 107
Size: 212 4302
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 660 2464 2645 2808 660
Size: 1804 181 163 2366
Fragments arranged by size:
2366 1804 181 163
BtsI GCAGTG_nn'
Cuts at: 386 1757 386
Size: 1371 3143
DraI TTT'AAA
Cuts at: 170 1513 170
Size: 1343 3171
DrdI GACnn_nn'nnGTC
Cuts at: 2220 3078 2220
Size: 858 3656
EaeI y'GGCC_r
Cuts at: 306 2099 2273 2664 2691 4036 306
Size: 1793 174 391 27 1345 784
Fragments arranged by size:
1793 1345 784 391 174 27
EagI C'GGCC_G
Cuts at: 2099 2099
Size: 4514
EarI CTCTTCn'nnn_
Cuts at: 137 212 2537 2747 137
Size: 75 2325 210 1904
Fragments arranged by size:
2325 1904 210 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2722 3032 3178 4324 2722
Size: 310 146 1146 2912
Fragments arranged by size:
2912 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1526 1526
Size: 4514
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 775 784 1234 2339 2771 3518 3786 775
Size: 9 450 1105 432 747 268 1503
Fragments arranged by size:
1503 1105 747 450 432 268 9
EcoO109I rG'GnC_Cy
Cuts at: 963 1266 1653 963
Size: 303 387 3824
Fragments arranged by size:
3824 387 303
EcoRI G'AATT_C
Cuts at: 1518 1518
Size: 4514
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 582 2533 582
Size: 1951 2563
FspI TGC'GCA
Cuts at: 2295 2295
Size: 4514
HaeII r_GCGC'y
Cuts at: 551 1098 1457 1948 2196 3218 4197 551
Size: 547 359 491 248 1022 979 868
Fragments arranged by size:
1022 979 868 547 491 359 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 240 272 730 762 2356 2386 2388 2418 240
Size: 32 458 32 1594 30 2 30 2336
Fragments arranged by size:
2336 1594 458 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1553 1553
Size: 4514
HindIII A'AGCT_T
Cuts at: 1205 1205
Size: 4514
KasI G'GCGC_C
Cuts at: 547 1944 2192 547
Size: 1397 248 2869
Fragments arranged by size:
2869 1397 248
KpnI G_GTAC'C
Cuts at: 319 1534 319
Size: 1215 3299
MluI A'CGCG_T
Cuts at: 3947 3947
Size: 4514
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 407 3185 3369 407
Size: 2778 184 1552
Fragments arranged by size:
2778 1552 184
MscI TGG'CCA
Cuts at: 308 2275 308
Size: 1967 2547
MslI CAynn'nnrTG
Cuts at: 170 401 1063 2503 2630 170
Size: 231 662 1440 127 2054
Fragments arranged by size:
2054 1440 662 231 127
MspA1I CmG'CkG
Cuts at: 1625 1939 2299 3312 3557 1625
Size: 314 360 1013 245 2582
Fragments arranged by size:
2582 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2695 2695
Size: 4514
NarI GG'CG_CC
Cuts at: 548 1945 2193 548
Size: 1397 248 2869
Fragments arranged by size:
2869 1397 248
NcoI C'CATG_G
Cuts at: 107 319 107
Size: 212 4302
NgoMIV G'CCGG_C
Cuts at: 2693 2693
Size: 4514
NspI r_CATG'y
Cuts at: 817 1068 1567 2067 2974 817
Size: 251 499 500 907 2357
Fragments arranged by size:
2357 907 500 499 251
PacI TTA_AT'TAA
Cuts at: 161 161
Size: 4514
PasI CC'CwG_GG
Cuts at: 1085 1085
Size: 4514
PciI A'CATG_T
Cuts at: 2970 2970
Size: 4514
PmeI GTTT'AAAC
Cuts at: 1513 1513
Size: 4514
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 734 766 3653 3685 734
Size: 32 2887 32 1563
Fragments arranged by size:
2887 1563 32 32
PpuMI rG'GwC_Cy
Cuts at: 963 963
Size: 4514
PshAI GACnn'nnGTC
Cuts at: 1178 1178
Size: 4514
PstI C_TGCA'G
Cuts at: 1180 1561 2246 1180
Size: 381 685 3448
Fragments arranged by size:
3448 685 381
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4514
PvuII CAG'CTG
Cuts at: 1939 2299 1939
Size: 360 4154
RsrII CG'GwC_CG
Cuts at: 2709 2709
Size: 4514
SacI G_AGCT'C
Cuts at: 1528 1528
Size: 4514
SalI G'TCGA_C
Cuts at: 1551 1551
Size: 4514
SapI GCTCTTCn'nnn_
Cuts at: 212 2537 2747 212
Size: 2325 210 1979
Fragments arranged by size:
2325 1979 210
SbfI CC_TGCA'GG
Cuts at: 1561 1561
Size: 4514
ScaI AGT'ACT
Cuts at: 4031 4031
Size: 4514
SfcI C'TryA_G
Cuts at: 33 1038 1176 1557 2242 3235 3426 33
Size: 1005 138 381 685 993 191 1121
Fragments arranged by size:
1121 1005 993 685 381 191 138
SfoI GGC'GCC
Cuts at: 549 1946 2194 549
Size: 1397 248 2869
Fragments arranged by size:
2869 1397 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4514
SmaI CCC'GGG
Cuts at: 1536 1536
Size: 4514
SmlI C'TyrA_G
Cuts at: 820 1420 1669 2584 3076 3338 820
Size: 600 249 915 492 262 1996
Fragments arranged by size:
1996 915 600 492 262 249
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4514
SphI G_CATG'C
Cuts at: 1567 2067 1567
Size: 500 4014
SspI AAT'ATT
Cuts at: 522 522
Size: 4514
StyI C'CwwG_G
Cuts at: 107 319 1648 2002 107
Size: 212 1329 354 2619
Fragments arranged by size:
2619 1329 354 212
SwaI ATTT'AAAT
Cuts at: 170 170
Size: 4514
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 792 955 1077 2097 2230 2689 2898 792
Size: 163 122 1020 133 459 209 2408
Fragments arranged by size:
2408 1020 459 209 163 133 122
TatI w'GTAC_w
Cuts at: 113 1127 4029 113
Size: 1014 2902 598
Fragments arranged by size:
2902 1014 598
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4031 4063 4031
Size: 32 4482
Tth111I GACn'n_nGTC
Cuts at: 2311 2311
Size: 4514
XbaI T'CTAG_A
Cuts at: 1545 1545
Size: 4514
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2009 2009
Size: 4514
XmaI C'CCGG_G
Cuts at: 1534 1534
Size: 4514
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PacI PasI
PciI PmeI PpiI PpuMI PshAI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI ApaI AscI AvrII BbvCI
BcgI BfrBI BglI BmgBI BmtI Bpu10I BseRI BsiWI
BsmI BsmBI BssHII BstEII BstXI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PflMI PfoI PmlI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI XhoI XmnI ZraI