(Circular) (Six-base) MAPSORT of: pF1KB0411.seq Check: 5955 from: 1 to: 4514 pF1KB0411 4514 bp With 182 enzymes: SgfI * October 7, 2009 14:13 .. AccI GT'mk_AC Cuts at: 563 1552 563 Size: 989 3525 Acc65I G'GTAC_C Cuts at: 315 1530 315 Size: 1215 3299 AclI AA'CG_TT Cuts at: 4354 4354 Size: 4514 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 2339 2771 3518 3786 2339 Size: 432 747 268 3067 Fragments arranged by size: 3067 747 432 268 AfeI AGC'GCT Cuts at: 1096 1096 Size: 4514 AflIII A'CryG_T Cuts at: 1866 2970 3947 1866 Size: 1104 977 2433 Fragments arranged by size: 2433 1104 977 AgeI A'CCGG_T Cuts at: 2902 2902 Size: 4514 AhdI GACnn_n'nnGTC Cuts at: 254 254 Size: 4514 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1191 1354 1191 Size: 163 4351 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 510 542 734 766 510 Size: 32 192 32 4258 Fragments arranged by size: 4258 192 32 32 AlwNI CAG_nnn'CTG Cuts at: 1308 1819 3386 1308 Size: 511 1567 2436 Fragments arranged by size: 2436 1567 511 ApaLI G'TGCA_C Cuts at: 1389 3284 1389 Size: 1895 2619 ApoI r'AATT_y Cuts at: 428 787 1518 3867 4123 428 Size: 359 731 2349 256 819 Fragments arranged by size: 2349 819 731 359 256 AseI AT'TA_AT Cuts at: 20 157 20 Size: 137 4377 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4514 AvaI C'yCGr_G Cuts at: 542 1534 542 Size: 992 3522 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 307 340 307 Size: 33 4481 BamHI G'GATC_C Cuts at: 1539 1539 Size: 4514 BanI G'GyrC_C Cuts at: 315 547 1075 1530 1944 2192 2227 315 Size: 232 528 455 414 248 35 2602 Fragments arranged by size: 2602 528 455 414 248 232 35 BanII G_rGCy'C Cuts at: 994 1528 2558 994 Size: 534 1030 2950 Fragments arranged by size: 2950 1030 534 BbeI G_GCGC'C Cuts at: 551 1948 2196 551 Size: 1397 248 2869 Fragments arranged by size: 2869 1397 248 BbsI GAAGACnn'nnnn_ Cuts at: 1166 1166 Size: 4514 BciVI GTATCCnnnnn_n' Cuts at: 2412 3173 4514 2412 Size: 761 1341 2412 Fragments arranged by size: 2412 1341 761 BclI T'GATC_A Cuts at: 2034 2034 Size: 4514 BglII A'GATC_T Cuts at: 2029 4066 2029 Size: 2037 2477 BlpI GC'TnA_GC Cuts at: 1107 1626 1107 Size: 519 3995 Bme1580I G_kGCm'C Cuts at: 1078 1393 2139 2232 3288 1078 Size: 315 746 93 1056 2304 Fragments arranged by size: 2304 1056 746 315 93 BmrI ACTGGGnnnn_n' Cuts at: 233 610 1726 1807 2142 4286 233 Size: 377 1116 81 335 2144 461 Fragments arranged by size: 2144 1116 461 377 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2881 2881 Size: 4514 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 775 784 1234 775 Size: 9 450 4055 Fragments arranged by size: 4055 450 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 805 1405 1690 2569 3061 3359 805 Size: 600 285 879 492 298 1960 Fragments arranged by size: 1960 879 600 492 298 285 BsaI GGTCTCn'nnnn_ Cuts at: 4218 4218 Size: 4514 BsaAI yAC'GTr Cuts at: 1867 1867 Size: 4514 BsaBI GATnn'nnATC Cuts at: 6 2028 4065 6 Size: 2022 2037 455 Fragments arranged by size: 2037 2022 455 BsaHI Gr'CG_yC Cuts at: 548 1945 2193 2895 4391 548 Size: 1397 248 702 1496 671 Fragments arranged by size: 1496 1397 702 671 248 BsaWI w'CCGG_w Cuts at: 1703 2224 2902 3176 3323 3734 1703 Size: 521 678 274 147 411 2483 Fragments arranged by size: 2483 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 734 764 734 Size: 30 4484 BseYI C'CCAG_C Cuts at: 1939 2697 3274 3770 1939 Size: 758 577 496 2683 Fragments arranged by size: 2683 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1029 1029 Size: 4514 BsiEI CG_ry'CG Cuts at: 104 2056 2102 3310 104 Size: 1952 46 1208 1308 Fragments arranged by size: 1952 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 124 1393 1528 2306 2496 3288 124 Size: 1269 135 778 190 792 1350 Fragments arranged by size: 1350 1269 792 778 190 135 Bsp1286I G_dGCh'C Cuts at: 124 994 1078 1393 1528 2139 2232 2306 2496 Size: 870 84 315 135 611 93 74 190 Cuts at: 2496 2558 3288 124 Size: 62 730 1350 Fragments arranged by size: 1350 870 730 611 315 190 135 93 84 74 62 BspEI T'CCGG_A Cuts at: 3734 3734 Size: 4514 BspHI T'CATG_A Cuts at: 861 3690 861 Size: 2829 1685 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1564 1778 2080 2461 1564 Size: 214 302 381 3617 Fragments arranged by size: 3617 381 302 214 BsrBI CCG'CTC Cuts at: 50 2806 2860 4344 4509 50 Size: 2756 54 1484 165 55 Fragments arranged by size: 2756 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1163 1373 2426 1163 Size: 210 1053 3251 Fragments arranged by size: 3251 1053 210 BsrFI r'CCGG_y Cuts at: 2512 2693 2902 2512 Size: 181 209 4124 Fragments arranged by size: 4124 209 181 BsrGI T'GTAC_A Cuts at: 1127 1127 Size: 4514 BssSI C'ACGA_G Cuts at: 667 2785 3143 667 Size: 2118 358 2038 Fragments arranged by size: 2118 2038 358 BstAPI GCAn_nnn'nTGC Cuts at: 1891 1891 Size: 4514 BstBI TT'CG_AA Cuts at: 2875 2875 Size: 4514 BstYI r'GATC_y Cuts at: 1 1539 2006 2029 2364 2610 3622 3708 3720 Size: 1538 467 23 335 246 1012 86 12 Cuts at: 3720 4066 1 Size: 346 449 Fragments arranged by size: 1538 1012 467 449 346 335 246 86 23 12 BstZ17I GTA'TAC Cuts at: 564 564 Size: 4514 Bsu36I CC'TnA_GG Cuts at: 959 959 Size: 4514 BtgI C'CryG_G Cuts at: 107 319 107 Size: 212 4302 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 660 2464 2645 2808 660 Size: 1804 181 163 2366 Fragments arranged by size: 2366 1804 181 163 BtsI GCAGTG_nn' Cuts at: 386 1757 386 Size: 1371 3143 DraI TTT'AAA Cuts at: 170 1513 170 Size: 1343 3171 DrdI GACnn_nn'nnGTC Cuts at: 2220 3078 2220 Size: 858 3656 EaeI y'GGCC_r Cuts at: 306 2099 2273 2664 2691 4036 306 Size: 1793 174 391 27 1345 784 Fragments arranged by size: 1793 1345 784 391 174 27 EagI C'GGCC_G Cuts at: 2099 2099 Size: 4514 EarI CTCTTCn'nnn_ Cuts at: 137 212 2537 2747 137 Size: 75 2325 210 1904 Fragments arranged by size: 2325 1904 210 75 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2722 3032 3178 4324 2722 Size: 310 146 1146 2912 Fragments arranged by size: 2912 1146 310 146 EcoICRI GAG'CTC Cuts at: 1526 1526 Size: 4514 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 775 784 1234 2339 2771 3518 3786 775 Size: 9 450 1105 432 747 268 1503 Fragments arranged by size: 1503 1105 747 450 432 268 9 EcoO109I rG'GnC_Cy Cuts at: 963 1266 1653 963 Size: 303 387 3824 Fragments arranged by size: 3824 387 303 EcoRI G'AATT_C Cuts at: 1518 1518 Size: 4514 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 582 2533 582 Size: 1951 2563 FspI TGC'GCA Cuts at: 2295 2295 Size: 4514 HaeII r_GCGC'y Cuts at: 551 1098 1457 1948 2196 3218 4197 551 Size: 547 359 491 248 1022 979 868 Fragments arranged by size: 1022 979 868 547 491 359 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 240 272 730 762 2356 2386 2388 2418 240 Size: 32 458 32 1594 30 2 30 2336 Fragments arranged by size: 2336 1594 458 32 32 30 30 2 HincII GTy'rAC Cuts at: 1553 1553 Size: 4514 HindIII A'AGCT_T Cuts at: 1205 1205 Size: 4514 KasI G'GCGC_C Cuts at: 547 1944 2192 547 Size: 1397 248 2869 Fragments arranged by size: 2869 1397 248 KpnI G_GTAC'C Cuts at: 319 1534 319 Size: 1215 3299 MluI A'CGCG_T Cuts at: 3947 3947 Size: 4514 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 407 3185 3369 407 Size: 2778 184 1552 Fragments arranged by size: 2778 1552 184 MscI TGG'CCA Cuts at: 308 2275 308 Size: 1967 2547 MslI CAynn'nnrTG Cuts at: 170 401 1063 2503 2630 170 Size: 231 662 1440 127 2054 Fragments arranged by size: 2054 1440 662 231 127 MspA1I CmG'CkG Cuts at: 1625 1939 2299 3312 3557 1625 Size: 314 360 1013 245 2582 Fragments arranged by size: 2582 1013 360 314 245 NaeI GCC'GGC Cuts at: 2695 2695 Size: 4514 NarI GG'CG_CC Cuts at: 548 1945 2193 548 Size: 1397 248 2869 Fragments arranged by size: 2869 1397 248 NcoI C'CATG_G Cuts at: 107 319 107 Size: 212 4302 NgoMIV G'CCGG_C Cuts at: 2693 2693 Size: 4514 NspI r_CATG'y Cuts at: 817 1068 1567 2067 2974 817 Size: 251 499 500 907 2357 Fragments arranged by size: 2357 907 500 499 251 PacI TTA_AT'TAA Cuts at: 161 161 Size: 4514 PasI CC'CwG_GG Cuts at: 1085 1085 Size: 4514 PciI A'CATG_T Cuts at: 2970 2970 Size: 4514 PmeI GTTT'AAAC Cuts at: 1513 1513 Size: 4514 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 734 766 3653 3685 734 Size: 32 2887 32 1563 Fragments arranged by size: 2887 1563 32 32 PpuMI rG'GwC_Cy Cuts at: 963 963 Size: 4514 PshAI GACnn'nnGTC Cuts at: 1178 1178 Size: 4514 PstI C_TGCA'G Cuts at: 1180 1561 2246 1180 Size: 381 685 3448 Fragments arranged by size: 3448 685 381 PvuI CG_AT'CG Cuts at: 104 104 Size: 4514 PvuII CAG'CTG Cuts at: 1939 2299 1939 Size: 360 4154 RsrII CG'GwC_CG Cuts at: 2709 2709 Size: 4514 SacI G_AGCT'C Cuts at: 1528 1528 Size: 4514 SalI G'TCGA_C Cuts at: 1551 1551 Size: 4514 SapI GCTCTTCn'nnn_ Cuts at: 212 2537 2747 212 Size: 2325 210 1979 Fragments arranged by size: 2325 1979 210 SbfI CC_TGCA'GG Cuts at: 1561 1561 Size: 4514 ScaI AGT'ACT Cuts at: 4031 4031 Size: 4514 SfcI C'TryA_G Cuts at: 33 1038 1176 1557 2242 3235 3426 33 Size: 1005 138 381 685 993 191 1121 Fragments arranged by size: 1121 1005 993 685 381 191 138 SfoI GGC'GCC Cuts at: 549 1946 2194 549 Size: 1397 248 2869 Fragments arranged by size: 2869 1397 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4514 SmaI CCC'GGG Cuts at: 1536 1536 Size: 4514 SmlI C'TyrA_G Cuts at: 820 1420 1669 2584 3076 3338 820 Size: 600 249 915 492 262 1996 Fragments arranged by size: 1996 915 600 492 262 249 SpeI A'CTAG_T Cuts at: 67 67 Size: 4514 SphI G_CATG'C Cuts at: 1567 2067 1567 Size: 500 4014 SspI AAT'ATT Cuts at: 522 522 Size: 4514 StyI C'CwwG_G Cuts at: 107 319 1648 2002 107 Size: 212 1329 354 2619 Fragments arranged by size: 2619 1329 354 212 SwaI ATTT'AAAT Cuts at: 170 170 Size: 4514 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 792 955 1077 2097 2230 2689 2898 792 Size: 163 122 1020 133 459 209 2408 Fragments arranged by size: 2408 1020 459 209 163 133 122 TatI w'GTAC_w Cuts at: 113 1127 4029 113 Size: 1014 2902 598 Fragments arranged by size: 2902 1014 598 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4031 4063 4031 Size: 32 4482 Tth111I GACn'n_nGTC Cuts at: 2311 2311 Size: 4514 XbaI T'CTAG_A Cuts at: 1545 1545 Size: 4514 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2009 2009 Size: 4514 XmaI C'CCGG_G Cuts at: 1534 1534 Size: 4514 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PacI PasI PciI PmeI PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AflII AleI ApaI AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BseRI BsiWI BsmI BsmBI BssHII BstEII BstXI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NruI NsiI PflMI PfoI PmlI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI XhoI XmnI ZraI