Restriction map for
pF1KB0413
(Circular) (Six-base) MAPSORT of: pF1KB0413.seq Check: 5264 from: 1 to: 4514
pF1KB0413 4514 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 224 493 224
Size: 269 4245
AccI GT'mk_AC
Cuts at: 1136 1552 1136
Size: 416 4098
Acc65I G'GTAC_C
Cuts at: 1530 1530
Size: 4514
AclI AA'CG_TT
Cuts at: 4354 4354
Size: 4514
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2339 2771 3518 3786 2339
Size: 432 747 268 3067
Fragments arranged by size:
3067 747 432 268
AflIII A'CryG_T
Cuts at: 1866 2970 3947 1866
Size: 1104 977 2433
Fragments arranged by size:
2433 1104 977
AgeI A'CCGG_T
Cuts at: 2902 2902
Size: 4514
AleI CACnn'nnGTG
Cuts at: 1261 1261
Size: 4514
AlwNI CAG_nnn'CTG
Cuts at: 264 814 882 1819 3386 264
Size: 550 68 937 1567 1392
Fragments arranged by size:
1567 1392 937 550 68
ApaI G_GGCC'C
Cuts at: 1393 1393
Size: 4514
ApaLI G'TGCA_C
Cuts at: 3284 3284
Size: 4514
ApoI r'AATT_y
Cuts at: 683 1518 3867 4123 683
Size: 835 2349 256 1074
Fragments arranged by size:
2349 1074 835 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4514
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4514
AvaI C'yCGr_G
Cuts at: 993 1534 993
Size: 541 3973
BamHI G'GATC_C
Cuts at: 534 1539 534
Size: 1005 3509
BanI G'GyrC_C
Cuts at: 169 188 310 643 937 1530 1944 2192 2227
Size: 19 122 333 294 593 414 248 35
Cuts at: 2227 169
Size: 2456
Fragments arranged by size:
2456 593 414 333 294 248 122 35
19
BanII G_rGCy'C
Cuts at: 1393 1528 2558 1393
Size: 135 1030 3349
Fragments arranged by size:
3349 1030 135
BbeI G_GCGC'C
Cuts at: 192 314 1948 2196 192
Size: 122 1634 248 2510
Fragments arranged by size:
2510 1634 248 122
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 274 308 415 449 274
Size: 34 107 34 4339
Fragments arranged by size:
4339 107 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2412 3173 4514 2412
Size: 761 1341 2412
Fragments arranged by size:
2412 1341 761
BclI T'GATC_A
Cuts at: 545 2034 545
Size: 1489 3025
BfrBI ATG'CAT
Cuts at: 426 678 426
Size: 252 4262
BglI GCCn_nnn'nGGC
Cuts at: 730 1484 730
Size: 754 3760
BglII A'GATC_T
Cuts at: 2029 4066 2029
Size: 2037 2477
BlpI GC'TnA_GC
Cuts at: 1626 1626
Size: 4514
Bme1580I G_kGCm'C
Cuts at: 174 297 646 1393 2139 2232 3288 174
Size: 123 349 747 746 93 1056 1400
Fragments arranged by size:
1400 1056 747 746 349 123 93
BmrI ACTGGGnnnn_n'
Cuts at: 133 471 1726 1807 2142 4286 133
Size: 338 1255 81 335 2144 361
Fragments arranged by size:
2144 1255 361 338 335 81
BmtI G_CTAG'C
Cuts at: 202 202
Size: 4514
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2881 2881
Size: 4514
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1690 2569 3061 3359 1690
Size: 879 492 298 2845
Fragments arranged by size:
2845 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 354 4218 354
Size: 3864 650
BsaAI yAC'GTr
Cuts at: 1867 1867
Size: 4514
BsaBI GATnn'nnATC
Cuts at: 6 2028 4065 6
Size: 2022 2037 455
Fragments arranged by size:
2037 2022 455
BsaHI Gr'CG_yC
Cuts at: 189 311 859 927 1433 1469 1945 2193 2895
Size: 122 548 68 506 36 476 248 702
Cuts at: 2895 4391 189
Size: 1496 312
Fragments arranged by size:
1496 702 548 506 476 312 248 122
68 36
BsaWI w'CCGG_w
Cuts at: 1703 2224 2902 3176 3323 3734 1703
Size: 521 678 274 147 411 2483
Fragments arranged by size:
2483 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1348 1348
Size: 4514
BseYI C'CCAG_C
Cuts at: 173 583 1392 1464 1939 2697 3274 3770 173
Size: 410 809 72 475 758 577 496 917
Fragments arranged by size:
917 809 758 577 496 475 410 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 806 196
Size: 610 3904
BsiEI CG_ry'CG
Cuts at: 104 279 828 2056 2102 3310 104
Size: 175 549 1228 46 1208 1308
Fragments arranged by size:
1308 1228 1208 549 175 46
BsiHKAI G_wGCw'C
Cuts at: 1528 2306 2496 3288 1528
Size: 778 190 792 2754
Fragments arranged by size:
2754 792 778 190
BsmI GAATG_Cn'
Cuts at: 626 626
Size: 4514
BsmBI CGTCTCn'nnnn_
Cuts at: 934 1001 1330 934
Size: 67 329 4118
Fragments arranged by size:
4118 329 67
Bsp1286I G_dGCh'C
Cuts at: 174 297 646 1393 1528 2139 2232 2306 2496
Size: 123 349 747 135 611 93 74 190
Cuts at: 2496 2558 3288 174
Size: 62 730 1400
Fragments arranged by size:
1400 747 730 611 349 190 135 123
93 74 62
BspEI T'CCGG_A
Cuts at: 3734 3734
Size: 4514
BspHI T'CATG_A
Cuts at: 668 759 1166 3690 668
Size: 91 407 2524 1492
Fragments arranged by size:
2524 1492 407 91
BspMI ACCTGCnnnn'nnnn_
Cuts at: 224 493 890 1564 1778 2080 2461 224
Size: 269 397 674 214 302 381 2277
Fragments arranged by size:
2277 674 397 381 302 269 214
BsrBI CCG'CTC
Cuts at: 50 2806 2860 4344 4509 50
Size: 2756 54 1484 165 55
Fragments arranged by size:
2756 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2426 2426
Size: 4514
BsrFI r'CCGG_y
Cuts at: 242 1292 2512 2693 2902 242
Size: 1050 1220 181 209 1854
Fragments arranged by size:
1854 1220 1050 209 181
BssSI C'ACGA_G
Cuts at: 2785 3143 2785
Size: 358 4156
BstAPI GCAn_nnn'nTGC
Cuts at: 1891 1891
Size: 4514
BstBI TT'CG_AA
Cuts at: 2875 2875
Size: 4514
BstYI r'GATC_y
Cuts at: 1 534 1539 2006 2029 2364 2610 3622 3708
Size: 533 1005 467 23 335 246 1012 86
Cuts at: 3708 3720 4066 1
Size: 12 346 449
Fragments arranged by size:
1012 1005 533 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 273 918 1052 273
Size: 645 134 3735
Fragments arranged by size:
3735 645 134
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 129 2464 2645 2808 129
Size: 2335 181 163 1835
Fragments arranged by size:
2335 1835 181 163
BtsI GCAGTG_nn'
Cuts at: 508 514 978 1076 1083 1757 508
Size: 6 464 98 7 674 3265
Fragments arranged by size:
3265 674 464 98 7 6
DraI TTT'AAA
Cuts at: 1513 1513
Size: 4514
DrdI GACnn_nn'nnGTC
Cuts at: 2220 3078 2220
Size: 858 3656
EaeI y'GGCC_r
Cuts at: 153 276 1376 2099 2273 2664 2691 4036 153
Size: 123 1100 723 174 391 27 1345 631
Fragments arranged by size:
1345 1100 723 631 391 174 123 27
EagI C'GGCC_G
Cuts at: 276 2099 276
Size: 1823 2691
EarI CTCTTCn'nnn_
Cuts at: 337 2537 2747 337
Size: 2200 210 2104
Fragments arranged by size:
2200 2104 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 640 2722 3032 3178 4324 640
Size: 2082 310 146 1146 830
Fragments arranged by size:
2082 1146 830 310 146
EcoICRI GAG'CTC
Cuts at: 1526 1526
Size: 4514
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2339 2771 3518 3786 2339
Size: 432 747 268 3067
Fragments arranged by size:
3067 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 619 1207 1234 619
Size: 588 27 3899
Fragments arranged by size:
3899 588 27
EcoO109I rG'GnC_Cy
Cuts at: 261 1301 1389 1653 261
Size: 1040 88 264 3122
Fragments arranged by size:
3122 1040 264 88
EcoRI G'AATT_C
Cuts at: 1518 1518
Size: 4514
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2533 2533
Size: 4514
FspI TGC'GCA
Cuts at: 2295 2295
Size: 4514
HaeII r_GCGC'y
Cuts at: 192 314 599 1071 1948 2196 3218 4197 192
Size: 122 285 472 877 248 1022 979 509
Fragments arranged by size:
1022 979 877 509 472 285 248 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 422 454 817 849 2356 2386 2388 2418 422
Size: 32 363 32 1507 30 2 30 2518
Fragments arranged by size:
2518 1507 363 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1553 1553
Size: 4514
KasI G'GCGC_C
Cuts at: 188 310 1944 2192 188
Size: 122 1634 248 2510
Fragments arranged by size:
2510 1634 248 122
KpnI G_GTAC'C
Cuts at: 1534 1534
Size: 4514
MluI A'CGCG_T
Cuts at: 3947 3947
Size: 4514
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 377 1175 3185 3369 377
Size: 798 2010 184 1522
Fragments arranged by size:
2010 1522 798 184
MscI TGG'CCA
Cuts at: 155 2275 155
Size: 2120 2394
MslI CAynn'nnrTG
Cuts at: 673 1210 1261 2503 2630 673
Size: 537 51 1242 127 2557
Fragments arranged by size:
2557 1242 537 127 51
MspA1I CmG'CkG
Cuts at: 147 275 814 920 1625 1939 2299 3312 3557
Size: 128 539 106 705 314 360 1013 245
Cuts at: 3557 147
Size: 1104
Fragments arranged by size:
1104 1013 705 539 360 314 245 128
106
NaeI GCC'GGC
Cuts at: 1294 2695 1294
Size: 1401 3113
NarI GG'CG_CC
Cuts at: 189 311 1945 2193 189
Size: 122 1634 248 2510
Fragments arranged by size:
2510 1634 248 122
NcoI C'CATG_G
Cuts at: 1052 1052
Size: 4514
NgoMIV G'CCGG_C
Cuts at: 1292 2693 1292
Size: 1401 3113
NheI G'CTAG_C
Cuts at: 198 198
Size: 4514
NsiI A_TGCA'T
Cuts at: 428 680 428
Size: 252 4262
NspI r_CATG'y
Cuts at: 678 1567 2067 2974 678
Size: 889 500 907 2218
Fragments arranged by size:
2218 907 889 500
PasI CC'CwG_GG
Cuts at: 703 703
Size: 4514
PciI A'CATG_T
Cuts at: 2970 2970
Size: 4514
PflMI CCAn_nnn'nTGG
Cuts at: 846 1385 846
Size: 539 3975
PfoI T'CCnGG_A
Cuts at: 227 227
Size: 4514
PmeI GTTT'AAAC
Cuts at: 1513 1513
Size: 4514
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3653 3685 3653
Size: 32 4482
PpuMI rG'GwC_Cy
Cuts at: 261 1301 261
Size: 1040 3474
PshAI GACnn'nnGTC
Cuts at: 862 1499 862
Size: 637 3877
PspOMI G'GGCC_C
Cuts at: 1389 1389
Size: 4514
PstI C_TGCA'G
Cuts at: 1561 2246 1561
Size: 685 3829
PvuI CG_AT'CG
Cuts at: 104 828 104
Size: 724 3790
PvuII CAG'CTG
Cuts at: 814 1939 2299 814
Size: 1125 360 3029
Fragments arranged by size:
3029 1125 360
RsrII CG'GwC_CG
Cuts at: 2709 2709
Size: 4514
SacI G_AGCT'C
Cuts at: 1528 1528
Size: 4514
SacII CC_GC'GG
Cuts at: 276 921 276
Size: 645 3869
SalI G'TCGA_C
Cuts at: 1551 1551
Size: 4514
SanDI GG'GwC_CC
Cuts at: 1301 1301
Size: 4514
SapI GCTCTTCn'nnn_
Cuts at: 2537 2747 2537
Size: 210 4304
SbfI CC_TGCA'GG
Cuts at: 1561 1561
Size: 4514
ScaI AGT'ACT
Cuts at: 4031 4031
Size: 4514
SexAI A'CCwGG_T
Cuts at: 1121 1121
Size: 4514
SfcI C'TryA_G
Cuts at: 33 1248 1557 2242 3235 3426 33
Size: 1215 309 685 993 191 1121
Fragments arranged by size:
1215 1121 993 685 309 191
SfoI GGC'GCC
Cuts at: 190 312 1946 2194 190
Size: 122 1634 248 2510
Fragments arranged by size:
2510 1634 248 122
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4514
SmaI CCC'GGG
Cuts at: 1536 1536
Size: 4514
SmlI C'TyrA_G
Cuts at: 1669 2584 3076 3338 1669
Size: 915 492 262 2845
Fragments arranged by size:
2845 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4514
SphI G_CATG'C
Cuts at: 1567 2067 1567
Size: 500 4014
StuI AGG'CCT
Cuts at: 733 733
Size: 4514
StyI C'CwwG_G
Cuts at: 414 774 1052 1269 1648 2002 414
Size: 360 278 217 379 354 2926
Fragments arranged by size:
2926 379 360 354 278 217
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 157 654 913 2097 2230 2689 2898 157
Size: 497 259 1184 133 459 209 1773
Fragments arranged by size:
1773 1184 497 459 259 209 133
TatI w'GTAC_w
Cuts at: 4029 4029
Size: 4514
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4031 4063 4031
Size: 32 4482
Tth111I GACn'n_nGTC
Cuts at: 2311 2311
Size: 4514
XbaI T'CTAG_A
Cuts at: 1545 1545
Size: 4514
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 181 568 813 2009 181
Size: 387 245 1196 2686
Fragments arranged by size:
2686 1196 387 245
XmaI C'CCGG_G
Cuts at: 1534 1534
Size: 4514
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BmgBI BpmI Bpu10I BsaXI BsiWI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI PacI PmlI PsiI PspXI PsrI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI