Restriction map for
pF1KB0415
(Circular) (Six-base) MAPSORT of: pF1KB0415.seq Check: 1671 from: 1 to: 4517
pF1KB0415 4517 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AccI GT'mk_AC
Cuts at: 1555 1555
Size: 4517
Acc65I G'GTAC_C
Cuts at: 1533 1533
Size: 4517
AclI AA'CG_TT
Cuts at: 4357 4357
Size: 4517
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1125 1172 1318 2342 2774 3521 3789 1125
Size: 47 146 1024 432 747 268 1853
Fragments arranged by size:
1853 1024 747 432 268 146 47
AflIII A'CryG_T
Cuts at: 1869 2973 3950 1869
Size: 1104 977 2436
Fragments arranged by size:
2436 1104 977
AgeI A'CCGG_T
Cuts at: 1398 2905 1398
Size: 1507 3010
AleI CACnn'nnGTG
Cuts at: 784 1372 784
Size: 588 3929
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1001 1001
Size: 4517
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1072 1104 1072
Size: 32 4485
AlwNI CAG_nnn'CTG
Cuts at: 781 1822 3389 781
Size: 1041 1567 1909
Fragments arranged by size:
1909 1567 1041
ApaLI G'TGCA_C
Cuts at: 424 3287 424
Size: 2863 1654
ApoI r'AATT_y
Cuts at: 1521 3870 4126 1521
Size: 2349 256 1912
Fragments arranged by size:
2349 1912 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4517
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4517
AvaI C'yCGr_G
Cuts at: 249 448 1434 1537 249
Size: 199 986 103 3229
Fragments arranged by size:
3229 986 199 103
BamHI G'GATC_C
Cuts at: 1542 1542
Size: 4517
BanI G'GyrC_C
Cuts at: 1533 1947 2195 2230 1533
Size: 414 248 35 3820
Fragments arranged by size:
3820 414 248 35
BanII G_rGCy'C
Cuts at: 458 484 872 1030 1296 1474 1531 2561 458
Size: 26 388 158 266 178 57 1030 2414
Fragments arranged by size:
2414 1030 388 266 178 158 57 26
BbeI G_GCGC'C
Cuts at: 1951 2199 1951
Size: 248 4269
BbsI GAAGACnn'nnnn_
Cuts at: 272 590 1282 272
Size: 318 692 3507
Fragments arranged by size:
3507 692 318
BbvCI CC'TCA_GC
Cuts at: 355 487 355
Size: 132 4385
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 189 223 90
Size: 34 65 34 4384
Fragments arranged by size:
4384 65 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2415 3176 4517 2415
Size: 761 1341 2415
Fragments arranged by size:
2415 1341 761
BclI T'GATC_A
Cuts at: 2037 2037
Size: 4517
BfrBI ATG'CAT
Cuts at: 1320 1320
Size: 4517
BglI GCCn_nnn'nGGC
Cuts at: 112 145 112
Size: 33 4484
BglII A'GATC_T
Cuts at: 2032 4069 2032
Size: 2037 2480
BlpI GC'TnA_GC
Cuts at: 1629 1629
Size: 4517
Bme1580I G_kGCm'C
Cuts at: 428 990 1045 2142 2235 3291 428
Size: 562 55 1097 93 1056 1654
Fragments arranged by size:
1654 1097 1056 562 93 55
BmgBI CAC'GTC
Cuts at: 1280 1280
Size: 4517
BmrI ACTGGGnnnn_n'
Cuts at: 294 1165 1366 1729 1810 2145 4289 294
Size: 871 201 363 81 335 2144 522
Fragments arranged by size:
2144 871 522 363 335 201 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2884 2884
Size: 4517
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 975 1055 1238 910
Size: 65 80 183 4189
Fragments arranged by size:
4189 183 80 65
Bpu10I CC'TnA_GC
Cuts at: 292 355 487 292
Size: 63 132 4322
Fragments arranged by size:
4322 132 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1693 2572 3064 3362 1693
Size: 879 492 298 2848
Fragments arranged by size:
2848 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4221 4221
Size: 4517
BsaAI yAC'GTr
Cuts at: 972 1870 972
Size: 898 3619
BsaBI GATnn'nnATC
Cuts at: 6 2031 4068 6
Size: 2025 2037 455
Fragments arranged by size:
2037 2025 455
BsaHI Gr'CG_yC
Cuts at: 950 1948 2196 2898 4394 950
Size: 998 248 702 1496 1073
Fragments arranged by size:
1496 1073 998 702 248
BsaWI w'CCGG_w
Cuts at: 473 1398 1706 2227 2905 3179 3326 3737 473
Size: 925 308 521 678 274 147 411 1253
Fragments arranged by size:
1253 925 678 521 411 308 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 218 248 817 847 218
Size: 30 569 30 3888
Fragments arranged by size:
3888 569 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 466 1009 1234 466
Size: 543 225 3749
Fragments arranged by size:
3749 543 225
BseYI C'CCAG_C
Cuts at: 319 411 415 458 1408 1942 2700 3277 3773
Size: 92 4 43 950 534 758 577 496
Cuts at: 3773 319
Size: 1063
Fragments arranged by size:
1063 950 758 577 534 496 92 43
4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 997 385
Size: 612 3905
BsiEI CG_ry'CG
Cuts at: 104 408 581 2059 2105 3313 104
Size: 304 173 1478 46 1208 1308
Fragments arranged by size:
1478 1308 1208 304 173 46
BsiHKAI G_wGCw'C
Cuts at: 293 428 523 551 872 1531 2309 2499 3291
Size: 135 95 28 321 659 778 190 792
Cuts at: 3291 293
Size: 1519
Fragments arranged by size:
1519 792 778 659 321 190 135 95
28
Bsp1286I G_dGCh'C
Cuts at: 293 428 458 484 523 551 872 990 1030
Size: 135 30 26 39 28 321 118 40
Cuts at: 1030 1045 1296 1474 1531 2142 2235 2309 2499
Size: 15 251 178 57 611 93 74 190
Cuts at: 2499 2561 3291 293
Size: 62 730 1519
Fragments arranged by size:
1519 730 611 321 251 190 178 135
118 93 74 62 57 40 39 30
28 26 15
BspEI T'CCGG_A
Cuts at: 473 3737 473
Size: 3264 1253
BspHI T'CATG_A
Cuts at: 3693 3693
Size: 4517
BspMI ACCTGCnnnn'nnnn_
Cuts at: 253 1567 1781 2083 2464 253
Size: 1314 214 302 381 2306
Fragments arranged by size:
2306 1314 381 302 214
BsrBI CCG'CTC
Cuts at: 50 160 959 2809 2863 4347 4512 50
Size: 110 799 1850 54 1484 165 55
Fragments arranged by size:
1850 1484 799 165 110 55 54
BsrDI GCAATG_nn'
Cuts at: 498 1250 2429 498
Size: 752 1179 2586
Fragments arranged by size:
2586 1179 752
BsrFI r'CCGG_y
Cuts at: 300 403 1398 2515 2696 2905 300
Size: 103 995 1117 181 209 1912
Fragments arranged by size:
1912 1117 995 209 181 103
BssHII G'CGCG_C
Cuts at: 125 125
Size: 4517
BssSI C'ACGA_G
Cuts at: 2788 3146 2788
Size: 358 4159
BstAPI GCAn_nnn'nTGC
Cuts at: 975 1894 975
Size: 919 3598
BstBI TT'CG_AA
Cuts at: 2878 2878
Size: 4517
BstXI CCAn_nnnn'nTGG
Cuts at: 379 1209 1416 379
Size: 830 207 3480
Fragments arranged by size:
3480 830 207
BstYI r'GATC_y
Cuts at: 1 1542 2009 2032 2367 2613 3625 3711 3723
Size: 1541 467 23 335 246 1012 86 12
Cuts at: 3723 4069 1
Size: 346 449
Fragments arranged by size:
1541 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 964 964
Size: 4517
BtgI C'CryG_G
Cuts at: 785 1037 1465 785
Size: 252 428 3837
Fragments arranged by size:
3837 428 252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2467 2648 2811 2467
Size: 181 163 4173
Fragments arranged by size:
4173 181 163
BtsI GCAGTG_nn'
Cuts at: 779 1195 1760 779
Size: 416 565 3536
Fragments arranged by size:
3536 565 416
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 994 1029 994
Size: 35 4482
DraI TTT'AAA
Cuts at: 1516 1516
Size: 4517
DraIII CAC_nnn'GTG
Cuts at: 288 975 288
Size: 687 3830
DrdI GACnn_nn'nnGTC
Cuts at: 2223 3081 2223
Size: 858 3659
EaeI y'GGCC_r
Cuts at: 113 146 405 578 788 2102 2276 2667 2694
Size: 33 259 173 210 1314 174 391 27
Cuts at: 2694 4039 113
Size: 1345 591
Fragments arranged by size:
1345 1314 591 391 259 210 174 173
33 27
EagI C'GGCC_G
Cuts at: 405 578 2102 405
Size: 173 1524 2820
Fragments arranged by size:
2820 1524 173
EarI CTCTTCn'nnn_
Cuts at: 472 1144 2540 2750 472
Size: 672 1396 210 2239
Fragments arranged by size:
2239 1396 672 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 749 1313 2725 3035 3181 4327 749
Size: 564 1412 310 146 1146 939
Fragments arranged by size:
1412 1146 939 564 310 146
EcoICRI GAG'CTC
Cuts at: 870 1529 870
Size: 659 3858
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 975 1055 1125 1172 1238 1318 2342 2774
Size: 65 80 70 47 66 80 1024 432
Cuts at: 2774 3521 3789 910
Size: 747 268 1638
Fragments arranged by size:
1638 1024 747 432 268 80 80 70
66 65 47
EcoO109I rG'GnC_Cy
Cuts at: 511 921 1461 1656 511
Size: 410 540 195 3372
Fragments arranged by size:
3372 540 410 195
EcoRI G'AATT_C
Cuts at: 1521 1521
Size: 4517
EcoRV GAT'ATC
Cuts at: 877 877
Size: 4517
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2536 2536
Size: 4517
FseI GG_CCGG'CC
Cuts at: 407 407
Size: 4517
FspI TGC'GCA
Cuts at: 188 2298 188
Size: 2110 2407
HaeII r_GCGC'y
Cuts at: 166 180 1951 2199 3221 4200 166
Size: 14 1771 248 1022 979 483
Fragments arranged by size:
1771 1022 979 483 248 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 817 849 1417 1449 2359 2389 2391 2421 817
Size: 32 568 32 910 30 2 30 2913
Fragments arranged by size:
2913 910 568 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1556 1556
Size: 4517
KasI G'GCGC_C
Cuts at: 1947 2195 1947
Size: 248 4269
KpnI G_GTAC'C
Cuts at: 1537 1537
Size: 4517
MluI A'CGCG_T
Cuts at: 3950 3950
Size: 4517
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3188 3372 3188
Size: 184 4333
MscI TGG'CCA
Cuts at: 790 2278 790
Size: 1488 3029
MslI CAynn'nnrTG
Cuts at: 784 935 1096 1372 2506 2633 784
Size: 151 161 276 1134 127 2668
Fragments arranged by size:
2668 1134 276 161 151 127
MspA1I CmG'CkG
Cuts at: 415 803 1075 1344 1628 1942 2302 3315 3560
Size: 388 272 269 284 314 360 1013 245
Cuts at: 3560 415
Size: 1372
Fragments arranged by size:
1372 1013 388 360 314 284 272 269
245
NaeI GCC'GGC
Cuts at: 302 405 2698 302
Size: 103 2293 2121
Fragments arranged by size:
2293 2121 103
NarI GG'CG_CC
Cuts at: 1948 2196 1948
Size: 248 4269
NcoI C'CATG_G
Cuts at: 1037 1037
Size: 4517
NgoMIV G'CCGG_C
Cuts at: 300 403 2696 300
Size: 103 2293 2121
Fragments arranged by size:
2293 2121 103
NsiI A_TGCA'T
Cuts at: 1322 1322
Size: 4517
NspI r_CATG'y
Cuts at: 1320 1570 2070 2977 1320
Size: 250 500 907 2860
Fragments arranged by size:
2860 907 500 250
PciI A'CATG_T
Cuts at: 2973 2973
Size: 4517
PflMI CCAn_nnn'nTGG
Cuts at: 326 326
Size: 4517
PfoI T'CCnGG_A
Cuts at: 590 647 835 1060 1349 590
Size: 57 188 225 289 3758
Fragments arranged by size:
3758 289 225 188 57
PmeI GTTT'AAAC
Cuts at: 1516 1516
Size: 4517
PmlI CAC'GTG
Cuts at: 972 972
Size: 4517
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3656 3688 3656
Size: 32 4485
PpuMI rG'GwC_Cy
Cuts at: 511 921 1461 511
Size: 410 540 3567
Fragments arranged by size:
3567 540 410
PspXI vC'TCGA_Gb
Cuts at: 1434 1434
Size: 4517
PstI C_TGCA'G
Cuts at: 309 1564 2249 309
Size: 1255 685 2577
Fragments arranged by size:
2577 1255 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4517
PvuII CAG'CTG
Cuts at: 415 1075 1344 1942 2302 415
Size: 660 269 598 360 2630
Fragments arranged by size:
2630 660 598 360 269
RsrII CG'GwC_CG
Cuts at: 2712 2712
Size: 4517
SacI G_AGCT'C
Cuts at: 872 1531 872
Size: 659 3858
SalI G'TCGA_C
Cuts at: 1554 1554
Size: 4517
SanDI GG'GwC_CC
Cuts at: 1461 1461
Size: 4517
SapI GCTCTTCn'nnn_
Cuts at: 472 2540 2750 472
Size: 2068 210 2239
Fragments arranged by size:
2239 2068 210
SbfI CC_TGCA'GG
Cuts at: 1564 1564
Size: 4517
ScaI AGT'ACT
Cuts at: 4034 4034
Size: 4517
SfcI C'TryA_G
Cuts at: 33 305 852 1560 2245 3238 3429 33
Size: 272 547 708 685 993 191 1121
Fragments arranged by size:
1121 993 708 685 547 272 191
SfoI GGC'GCC
Cuts at: 1949 2197 1949
Size: 248 4269
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4517
SgrAI Cr'CCGG_yG
Cuts at: 1398 1398
Size: 4517
SmaI CCC'GGG
Cuts at: 251 1539 251
Size: 1288 3229
SmlI C'TyrA_G
Cuts at: 1434 1672 2587 3079 3341 1434
Size: 238 915 492 262 2610
Fragments arranged by size:
2610 915 492 262 238
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4517
SphI G_CATG'C
Cuts at: 1320 1570 2070 1320
Size: 250 500 3767
Fragments arranged by size:
3767 500 250
SspI AAT'ATT
Cuts at: 1134 1134
Size: 4517
StuI AGG'CCT
Cuts at: 1359 1359
Size: 4517
StyI C'CwwG_G
Cuts at: 172 1037 1048 1651 2005 172
Size: 865 11 603 354 2684
Fragments arranged by size:
2684 865 603 354 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 276 2100 2233 2692 2901 276
Size: 1824 133 459 209 1892
Fragments arranged by size:
1892 1824 459 209 133
TatI w'GTAC_w
Cuts at: 4032 4032
Size: 4517
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 218 250 4034 4066 218
Size: 32 3784 32 669
Fragments arranged by size:
3784 669 32 32
Tth111I GACn'n_nGTC
Cuts at: 2314 2314
Size: 4517
XbaI T'CTAG_A
Cuts at: 1548 1548
Size: 4517
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2012 2012
Size: 4517
XhoI C'TCGA_G
Cuts at: 1434 1434
Size: 4517
XmaI C'CCGG_G
Cuts at: 249 1537 249
Size: 1288 3229
XmnI GAAnn'nnTTC
Cuts at: 387 387
Size: 4517
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspXI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI ApaI AscI AvrII
BaeI BmtI BsiWI BsmI BsmBI BsrGI BstEII BstZ17I
ClaI EcoNI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PasI PshAI PsiI PspOMI PsrI
SacII SexAI SfiI SnaBI SrfI SwaI ZraI