Restriction map for
pF1KB0419
(Circular) (Six-base) MAPSORT of: pF1KB0419.seq Check: 9940 from: 1 to: 4529
pF1KB0419 4529 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AccI GT'mk_AC
Cuts at: 1567 1567
Size: 4529
Acc65I G'GTAC_C
Cuts at: 1545 1545
Size: 4529
AclI AA'CG_TT
Cuts at: 377 4369 377
Size: 3992 537
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 695 2354 2786 3533 3801 695
Size: 1659 432 747 268 1423
Fragments arranged by size:
1659 1423 747 432 268
AflIII A'CryG_T
Cuts at: 1881 2985 3962 1881
Size: 1104 977 2448
Fragments arranged by size:
2448 1104 977
AgeI A'CCGG_T
Cuts at: 2917 2917
Size: 4529
AlwNI CAG_nnn'CTG
Cuts at: 1353 1834 3401 1353
Size: 481 1567 2481
Fragments arranged by size:
2481 1567 481
ApaI G_GGCC'C
Cuts at: 1339 1339
Size: 4529
ApaLI G'TGCA_C
Cuts at: 3299 3299
Size: 4529
ApoI r'AATT_y
Cuts at: 212 1191 1251 1533 3882 4138 212
Size: 979 60 282 2349 256 603
Fragments arranged by size:
2349 979 603 282 256 60
AseI AT'TA_AT
Cuts at: 20 20
Size: 4529
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4529
AvaI C'yCGr_G
Cuts at: 1549 1549
Size: 4529
BamHI G'GATC_C
Cuts at: 1554 1554
Size: 4529
BanI G'GyrC_C
Cuts at: 1545 1959 2207 2242 1545
Size: 414 248 35 3832
Fragments arranged by size:
3832 414 248 35
BanII G_rGCy'C
Cuts at: 228 1339 1543 2573 228
Size: 1111 204 1030 2184
Fragments arranged by size:
2184 1111 1030 204
BbeI G_GCGC'C
Cuts at: 1963 2211 1963
Size: 248 4281
BciVI GTATCCnnnnn_n'
Cuts at: 1396 2427 3188 4529 1396
Size: 1031 761 1341 1396
Fragments arranged by size:
1396 1341 1031 761
BclI T'GATC_A
Cuts at: 924 2049 924
Size: 1125 3404
BglII A'GATC_T
Cuts at: 2044 4081 2044
Size: 2037 2492
BlpI GC'TnA_GC
Cuts at: 1641 1641
Size: 4529
Bme1580I G_kGCm'C
Cuts at: 121 641 1339 2154 2247 3303 121
Size: 520 698 815 93 1056 1347
Fragments arranged by size:
1347 1056 815 698 520 93
BmrI ACTGGGnnnn_n'
Cuts at: 1741 1822 2157 4301 1741
Size: 81 335 2144 1969
Fragments arranged by size:
2144 1969 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2896 2896
Size: 4529
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 1705 2584 3076 3374 212
Size: 1493 879 492 298 1367
Fragments arranged by size:
1493 1367 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 887 4233 887
Size: 3346 1183
BsaAI yAC'GTr
Cuts at: 1882 1882
Size: 4529
BsaBI GATnn'nnATC
Cuts at: 6 2043 4080 6
Size: 2037 2037 455
Fragments arranged by size:
2037 2037 455
BsaHI Gr'CG_yC
Cuts at: 1960 2208 2910 4406 1960
Size: 248 702 1496 2083
Fragments arranged by size:
2083 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1718 2239 2917 3191 3338 3749 1718
Size: 521 678 274 147 411 2498
Fragments arranged by size:
2498 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 570 794 570
Size: 224 4305
BseYI C'CCAG_C
Cuts at: 1338 1954 2712 3289 3785 1338
Size: 616 758 577 496 2082
Fragments arranged by size:
2082 758 616 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 598 208
Size: 390 4139
BsiEI CG_ry'CG
Cuts at: 104 179 2071 2117 3325 104
Size: 75 1892 46 1208 1308
Fragments arranged by size:
1892 1308 1208 75 46
BsiHKAI G_wGCw'C
Cuts at: 964 1543 2321 2511 3303 964
Size: 579 778 190 792 2190
Fragments arranged by size:
2190 792 778 579 190
Bsp1286I G_dGCh'C
Cuts at: 121 228 641 964 1339 1543 2154 2247 2321
Size: 107 413 323 375 204 611 93 74
Cuts at: 2321 2511 2573 3303 121
Size: 190 62 730 1347
Fragments arranged by size:
1347 730 611 413 375 323 204 190
107 93 74 62
BspEI T'CCGG_A
Cuts at: 3749 3749
Size: 4529
BspHI T'CATG_A
Cuts at: 1119 3705 1119
Size: 2586 1943
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1579 1793 2095 2476 1579
Size: 214 302 381 3632
Fragments arranged by size:
3632 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1041 2821 2875 4359 4524 50
Size: 991 1780 54 1484 165 55
Fragments arranged by size:
1780 1484 991 165 55 54
BsrDI GCAATG_nn'
Cuts at: 532 2441 532
Size: 1909 2620
BsrFI r'CCGG_y
Cuts at: 537 2527 2708 2917 537
Size: 1990 181 209 2149
Fragments arranged by size:
2149 1990 209 181
BsrGI T'GTAC_A
Cuts at: 978 978
Size: 4529
BssSI C'ACGA_G
Cuts at: 2800 3158 2800
Size: 358 4171
BstAPI GCAn_nnn'nTGC
Cuts at: 1906 1906
Size: 4529
BstBI TT'CG_AA
Cuts at: 2890 2890
Size: 4529
BstEII G'GTnAC_C
Cuts at: 1408 1408
Size: 4529
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4529
BstYI r'GATC_y
Cuts at: 1 1554 2021 2044 2379 2625 3637 3723 3735
Size: 1553 467 23 335 246 1012 86 12
Cuts at: 3735 4081 1
Size: 346 449
Fragments arranged by size:
1553 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2479 2660 2823 2479
Size: 181 163 4185
Fragments arranged by size:
4185 181 163
BtsI GCAGTG_nn'
Cuts at: 1427 1772 1427
Size: 345 4184
DraI TTT'AAA
Cuts at: 1528 1528
Size: 4529
DrdI GACnn_nn'nnGTC
Cuts at: 2235 3093 2235
Size: 858 3671
EaeI y'GGCC_r
Cuts at: 176 428 713 724 1383 2114 2288 2679 2706
Size: 252 285 11 659 731 174 391 27
Cuts at: 2706 4051 176
Size: 1345 654
Fragments arranged by size:
1345 731 659 654 391 285 252 174
27 11
EagI C'GGCC_G
Cuts at: 176 2114 176
Size: 1938 2591
EarI CTCTTCn'nnn_
Cuts at: 885 1189 1249 2552 2762 885
Size: 304 60 1303 210 2652
Fragments arranged by size:
2652 1303 304 210 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2737 3047 3193 4339 2737
Size: 310 146 1146 2927
Fragments arranged by size:
2927 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1541 1541
Size: 4529
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 695 2354 2786 3533 3801 695
Size: 1659 432 747 268 1423
Fragments arranged by size:
1659 1423 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 730 1335 1668 730
Size: 605 333 3591
Fragments arranged by size:
3591 605 333
EcoRI G'AATT_C
Cuts at: 1191 1251 1533 1191
Size: 60 282 4187
Fragments arranged by size:
4187 282 60
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2548 2548
Size: 4529
FspI TGC'GCA
Cuts at: 2310 2310
Size: 4529
HaeII r_GCGC'y
Cuts at: 1963 2211 3233 4212 1963
Size: 248 1022 979 2280
Fragments arranged by size:
2280 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 931 963 2371 2401 2403 2433 931
Size: 32 1408 30 2 30 3027
Fragments arranged by size:
3027 1408 32 30 30 2
HincII GTy'rAC
Cuts at: 1568 1568
Size: 4529
HindIII A'AGCT_T
Cuts at: 706 706
Size: 4529
KasI G'GCGC_C
Cuts at: 1959 2207 1959
Size: 248 4281
KpnI G_GTAC'C
Cuts at: 1549 1549
Size: 4529
MluI A'CGCG_T
Cuts at: 3962 3962
Size: 4529
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 488 3200 3384 488
Size: 2712 184 1633
Fragments arranged by size:
2712 1633 184
MscI TGG'CCA
Cuts at: 715 1385 2290 715
Size: 670 905 2954
Fragments arranged by size:
2954 905 670
MslI CAynn'nnrTG
Cuts at: 350 1124 1172 1232 2518 2645 350
Size: 774 48 60 1286 127 2234
Fragments arranged by size:
2234 1286 774 127 60 48
MspA1I CmG'CkG
Cuts at: 723 1353 1640 1954 2314 3327 3572 723
Size: 630 287 314 360 1013 245 1680
Fragments arranged by size:
1680 1013 630 360 314 287 245
NaeI GCC'GGC
Cuts at: 2710 2710
Size: 4529
NarI GG'CG_CC
Cuts at: 1960 2208 1960
Size: 248 4281
NgoMIV G'CCGG_C
Cuts at: 2708 2708
Size: 4529
NspI r_CATG'y
Cuts at: 987 1497 1582 2082 2989 987
Size: 510 85 500 907 2527
Fragments arranged by size:
2527 907 510 500 85
PciI A'CATG_T
Cuts at: 2985 2985
Size: 4529
PflMI CCAn_nnn'nTGG
Cuts at: 1296 1296
Size: 4529
PmeI GTTT'AAAC
Cuts at: 1528 1528
Size: 4529
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3668 3700 3668
Size: 32 4497
PpuMI rG'GwC_Cy
Cuts at: 730 730
Size: 4529
PsiI TTA'TAA
Cuts at: 1025 1025
Size: 4529
PspOMI G'GGCC_C
Cuts at: 1335 1335
Size: 4529
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1417 1449 1417
Size: 32 4497
PstI C_TGCA'G
Cuts at: 1576 2261 1576
Size: 685 3844
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4529
PvuII CAG'CTG
Cuts at: 1353 1954 2314 1353
Size: 601 360 3568
Fragments arranged by size:
3568 601 360
RsrII CG'GwC_CG
Cuts at: 2724 2724
Size: 4529
SacI G_AGCT'C
Cuts at: 1543 1543
Size: 4529
SalI G'TCGA_C
Cuts at: 1566 1566
Size: 4529
SapI GCTCTTCn'nnn_
Cuts at: 2552 2762 2552
Size: 210 4319
SbfI CC_TGCA'GG
Cuts at: 1576 1576
Size: 4529
ScaI AGT'ACT
Cuts at: 4046 4046
Size: 4529
SfcI C'TryA_G
Cuts at: 33 1196 1256 1437 1572 2257 3250 3441 33
Size: 1163 60 181 135 685 993 191 1121
Fragments arranged by size:
1163 1121 993 685 191 181 135 60
SfoI GGC'GCC
Cuts at: 1961 2209 1961
Size: 248 4281
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4529
SmaI CCC'GGG
Cuts at: 1551 1551
Size: 4529
SmlI C'TyrA_G
Cuts at: 227 1684 2599 3091 3353 227
Size: 1457 915 492 262 1403
Fragments arranged by size:
1457 1403 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4529
SphI G_CATG'C
Cuts at: 1582 2082 1582
Size: 500 4029
StuI AGG'CCT
Cuts at: 571 571
Size: 4529
StyI C'CwwG_G
Cuts at: 1413 1663 2017 1413
Size: 250 354 3925
Fragments arranged by size:
3925 354 250
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 381 2112 2245 2704 2913 381
Size: 1731 133 459 209 1997
Fragments arranged by size:
1997 1731 459 209 133
TatI w'GTAC_w
Cuts at: 978 1056 4044 978
Size: 78 2988 1463
Fragments arranged by size:
2988 1463 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 128 160 789 821 4046 4078 128
Size: 32 629 32 3225 32 579
Fragments arranged by size:
3225 629 579 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2326 2326
Size: 4529
XbaI T'CTAG_A
Cuts at: 1560 1560
Size: 4529
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1354 2024 1354
Size: 670 3859
XmaI C'CCGG_G
Cuts at: 1549 1549
Size: 4529
XmnI GAAnn'nnTTC
Cuts at: 488 488
Size: 4529
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PmeI PpiI PpuMI PsiI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsmBI
BssHII BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PshAI
PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI