Restriction map for
pF1KB0421
(Circular) (Six-base) MAPSORT of: pF1KB0421.seq Check: 8182 from: 1 to: 4343
pF1KB0421 4343 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AccI GT'mk_AC
Cuts at: 1085 1381 1085
Size: 296 4047
Acc65I G'GTAC_C
Cuts at: 1359 1359
Size: 4343
AclI AA'CG_TT
Cuts at: 4183 4183
Size: 4343
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 612 1154 2168 2600 3347 3615 612
Size: 542 1014 432 747 268 1340
Fragments arranged by size:
1340 1014 747 542 432 268
AflIII A'CryG_T
Cuts at: 1695 2799 3776 1695
Size: 1104 977 2262
Fragments arranged by size:
2262 1104 977
AgeI A'CCGG_T
Cuts at: 2731 2731
Size: 4343
AhdI GACnn_n'nnGTC
Cuts at: 694 694
Size: 4343
AlwNI CAG_nnn'CTG
Cuts at: 635 1174 1648 3215 635
Size: 539 474 1567 1763
Fragments arranged by size:
1763 1567 539 474
ApaI G_GGCC'C
Cuts at: 1251 1251
Size: 4343
ApaLI G'TGCA_C
Cuts at: 3113 3113
Size: 4343
ApoI r'AATT_y
Cuts at: 1347 3696 3952 1347
Size: 2349 256 1738
Fragments arranged by size:
2349 1738 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4343
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4343
AvaI C'yCGr_G
Cuts at: 241 485 1363 241
Size: 244 878 3221
Fragments arranged by size:
3221 878 244
BamHI G'GATC_C
Cuts at: 245 1368 245
Size: 1123 3220
BanI G'GyrC_C
Cuts at: 1359 1773 2021 2056 1359
Size: 414 248 35 3646
Fragments arranged by size:
3646 414 248 35
BanII G_rGCy'C
Cuts at: 332 620 1251 1326 1357 2387 332
Size: 288 631 75 31 1030 2288
Fragments arranged by size:
2288 1030 631 288 75 31
BbeI G_GCGC'C
Cuts at: 1777 2025 1777
Size: 248 4095
BbsI GAAGACnn'nnnn_
Cuts at: 246 947 1142 1232 246
Size: 701 195 90 3357
Fragments arranged by size:
3357 701 195 90
BbvCI CC'TCA_GC
Cuts at: 1211 1211
Size: 4343
BciVI GTATCCnnnnn_n'
Cuts at: 2241 3002 4343 2241
Size: 761 1341 2241
Fragments arranged by size:
2241 1341 761
BclI T'GATC_A
Cuts at: 1863 1863
Size: 4343
BglI GCCn_nnn'nGGC
Cuts at: 138 1330 138
Size: 1192 3151
BglII A'GATC_T
Cuts at: 1858 3895 1858
Size: 2037 2306
BlpI GC'TnA_GC
Cuts at: 1455 1455
Size: 4343
Bme1580I G_kGCm'C
Cuts at: 1251 1968 2061 3117 1251
Size: 717 93 1056 2477
Fragments arranged by size:
2477 1056 717 93
BmgBI CAC'GTC
Cuts at: 252 252
Size: 4343
BmrI ACTGGGnnnn_n'
Cuts at: 936 945 1555 1636 1971 4115 936
Size: 9 610 81 335 2144 1164
Fragments arranged by size:
2144 1164 610 335 81 9
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 135 2710 135
Size: 2575 1768
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 872 872
Size: 4343
Bpu10I CC'TnA_GC
Cuts at: 1211 1211
Size: 4343
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 1129 1519 2398 2890 3188 517
Size: 612 390 879 492 298 1672
Fragments arranged by size:
1672 879 612 492 390 298
BsaI GGTCTCn'nnnn_
Cuts at: 488 681 702 732 1055 4047 488
Size: 193 21 30 323 2992 784
Fragments arranged by size:
2992 784 323 193 30 21
BsaAI yAC'GTr
Cuts at: 1696 1696
Size: 4343
BsaBI GATnn'nnATC
Cuts at: 6 1857 3894 6
Size: 1851 2037 455
Fragments arranged by size:
2037 1851 455
BsaHI Gr'CG_yC
Cuts at: 1774 2022 2724 4220 1774
Size: 248 702 1496 1897
Fragments arranged by size:
1897 1496 702 248
BsaWI w'CCGG_w
Cuts at: 352 1532 2053 2731 3005 3152 3563 352
Size: 1180 521 678 274 147 411 1132
Fragments arranged by size:
1180 1132 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 211 560 835 211
Size: 349 275 3719
Fragments arranged by size:
3719 349 275
BseYI C'CCAG_C
Cuts at: 466 1189 1768 2526 3103 3599 466
Size: 723 579 758 577 496 1210
Fragments arranged by size:
1210 758 723 579 577 496
BsiEI CG_ry'CG
Cuts at: 104 1885 1931 3139 104
Size: 1781 46 1208 1308
Fragments arranged by size:
1781 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 908 1357 2135 2325 3117 908
Size: 449 778 190 792 2134
Fragments arranged by size:
2134 792 778 449 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1311 1311
Size: 4343
Bsp1286I G_dGCh'C
Cuts at: 332 620 908 1251 1326 1357 1968 2061 2135
Size: 288 288 343 75 31 611 93 74
Cuts at: 2135 2325 2387 3117 332
Size: 190 62 730 1558
Fragments arranged by size:
1558 730 611 343 288 288 190 93
75 74 62 31
BspEI T'CCGG_A
Cuts at: 352 3563 352
Size: 3211 1132
BspHI T'CATG_A
Cuts at: 3519 3519
Size: 4343
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1393 1607 1909 2290 1393
Size: 214 302 381 3446
Fragments arranged by size:
3446 381 302 214
BsrBI CCG'CTC
Cuts at: 50 138 2635 2689 4173 4338 50
Size: 88 2497 54 1484 165 55
Fragments arranged by size:
2497 1484 165 88 55 54
BsrDI GCAATG_nn'
Cuts at: 2255 2255
Size: 4343
BsrFI r'CCGG_y
Cuts at: 155 2341 2522 2731 155
Size: 2186 181 209 1767
Fragments arranged by size:
2186 1767 209 181
BsrGI T'GTAC_A
Cuts at: 837 837
Size: 4343
BssHII G'CGCG_C
Cuts at: 269 269
Size: 4343
BssSI C'ACGA_G
Cuts at: 184 331 552 2614 2972 184
Size: 147 221 2062 358 1555
Fragments arranged by size:
2062 1555 358 221 147
BstAPI GCAn_nnn'nTGC
Cuts at: 1720 1720
Size: 4343
BstBI TT'CG_AA
Cuts at: 2704 2704
Size: 4343
BstEII G'GTnAC_C
Cuts at: 1203 1203
Size: 4343
BstXI CCAn_nnnn'nTGG
Cuts at: 861 861
Size: 4343
BstYI r'GATC_y
Cuts at: 1 245 1368 1835 1858 2193 2439 3451 3537
Size: 244 1123 467 23 335 246 1012 86
Cuts at: 3537 3549 3895 1
Size: 12 346 449
Fragments arranged by size:
1123 1012 467 449 346 335 246 244
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 120 587 120
Size: 467 3876
BtgI C'CryG_G
Cuts at: 107 107
Size: 4343
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 781 2293 2474 2637 781
Size: 1512 181 163 2487
Fragments arranged by size:
2487 1512 181 163
BtsI GCAGTG_nn'
Cuts at: 1586 1586
Size: 4343
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 760 795 760
Size: 35 4308
DraI TTT'AAA
Cuts at: 1342 1342
Size: 4343
DraIII CAC_nnn'GTG
Cuts at: 835 835
Size: 4343
DrdI GACnn_nn'nnGTC
Cuts at: 2049 2907 2049
Size: 858 3485
EaeI y'GGCC_r
Cuts at: 606 637 851 1067 1928 2102 2493 2520 3865
Size: 31 214 216 861 174 391 27 1345
Cuts at: 3865 606
Size: 1084
Fragments arranged by size:
1345 1084 861 391 216 214 174 31
27
EagI C'GGCC_G
Cuts at: 1928 1928
Size: 4343
EarI CTCTTCn'nnn_
Cuts at: 587 2366 2576 587
Size: 1779 210 2354
Fragments arranged by size:
2354 1779 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2551 2861 3007 4153 2551
Size: 310 146 1146 2741
Fragments arranged by size:
2741 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1355 1355
Size: 4343
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 612 872 1154 2168 2600 3347 3615 612
Size: 260 282 1014 432 747 268 1340
Fragments arranged by size:
1340 1014 747 432 282 268 260
EcoO109I rG'GnC_Cy
Cuts at: 378 1482 378
Size: 1104 3239
EcoRI G'AATT_C
Cuts at: 1347 1347
Size: 4343
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2362 2362
Size: 4343
FspI TGC'GCA
Cuts at: 2124 2124
Size: 4343
HaeII r_GCGC'y
Cuts at: 1777 2025 3047 4026 1777
Size: 248 1022 979 2094
Fragments arranged by size:
2094 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 680 712 2185 2215 2217 2247 680
Size: 32 1473 30 2 30 2776
Fragments arranged by size:
2776 1473 32 30 30 2
HincII GTy'rAC
Cuts at: 1382 1382
Size: 4343
KasI G'GCGC_C
Cuts at: 1773 2021 1773
Size: 248 4095
KpnI G_GTAC'C
Cuts at: 1363 1363
Size: 4343
MluI A'CGCG_T
Cuts at: 3776 3776
Size: 4343
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 413 3014 3198 413
Size: 2601 184 1558
Fragments arranged by size:
2601 1558 184
MscI TGG'CCA
Cuts at: 639 853 1069 2104 639
Size: 214 216 1035 2878
Fragments arranged by size:
2878 1035 216 214
MslI CAynn'nnrTG
Cuts at: 499 2332 2459 499
Size: 1833 127 2383
Fragments arranged by size:
2383 1833 127
MspA1I CmG'CkG
Cuts at: 266 846 892 1454 1768 2128 3141 3386 266
Size: 580 46 562 314 360 1013 245 1223
Fragments arranged by size:
1223 1013 580 562 360 314 245 46
NaeI GCC'GGC
Cuts at: 2524 2524
Size: 4343
NarI GG'CG_CC
Cuts at: 1774 2022 1774
Size: 248 4095
NcoI C'CATG_G
Cuts at: 107 107
Size: 4343
NgoMIV G'CCGG_C
Cuts at: 2522 2522
Size: 4343
NspI r_CATG'y
Cuts at: 1396 1896 2803 1396
Size: 500 907 2936
Fragments arranged by size:
2936 907 500
PciI A'CATG_T
Cuts at: 2799 2799
Size: 4343
PflMI CCAn_nnn'nTGG
Cuts at: 657 657
Size: 4343
PfoI T'CCnGG_A
Cuts at: 359 359
Size: 4343
PmeI GTTT'AAAC
Cuts at: 1342 1342
Size: 4343
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3482 3514 3482
Size: 32 4311
PpuMI rG'GwC_Cy
Cuts at: 378 378
Size: 4343
PspOMI G'GGCC_C
Cuts at: 1247 1247
Size: 4343
PstI C_TGCA'G
Cuts at: 1268 1390 2075 1268
Size: 122 685 3536
Fragments arranged by size:
3536 685 122
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4343
PvuII CAG'CTG
Cuts at: 266 846 892 1768 2128 266
Size: 580 46 876 360 2481
Fragments arranged by size:
2481 876 580 360 46
RsrII CG'GwC_CG
Cuts at: 2538 2538
Size: 4343
SacI G_AGCT'C
Cuts at: 1357 1357
Size: 4343
SalI G'TCGA_C
Cuts at: 1380 1380
Size: 4343
SapI GCTCTTCn'nnn_
Cuts at: 2366 2576 2366
Size: 210 4133
SbfI CC_TGCA'GG
Cuts at: 1268 1390 1268
Size: 122 4221
ScaI AGT'ACT
Cuts at: 3860 3860
Size: 4343
SfcI C'TryA_G
Cuts at: 33 438 1264 1386 2071 3064 3255 33
Size: 405 826 122 685 993 191 1121
Fragments arranged by size:
1121 993 826 685 405 191 122
SfoI GGC'GCC
Cuts at: 1775 2023 1775
Size: 248 4095
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4343
SmaI CCC'GGG
Cuts at: 1365 1365
Size: 4343
SmlI C'TyrA_G
Cuts at: 532 1108 1498 2413 2905 3167 532
Size: 576 390 915 492 262 1708
Fragments arranged by size:
1708 915 576 492 390 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4343
SphI G_CATG'C
Cuts at: 1396 1896 1396
Size: 500 3843
StyI C'CwwG_G
Cuts at: 107 854 1145 1477 1831 107
Size: 747 291 332 354 2619
Fragments arranged by size:
2619 747 354 332 291
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1036 1926 2059 2518 2727 1036
Size: 890 133 459 209 2652
Fragments arranged by size:
2652 890 459 209 133
TatI w'GTAC_w
Cuts at: 837 3858 837
Size: 3021 1322
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 300 332 543 575 737 769 3860 3892 300
Size: 32 211 32 162 32 3091 32 751
Fragments arranged by size:
3091 751 211 162 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 283 708 2140 283
Size: 425 1432 2486
Fragments arranged by size:
2486 1432 425
XbaI T'CTAG_A
Cuts at: 1374 1374
Size: 4343
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1838 1838
Size: 4343
XmaI C'CCGG_G
Cuts at: 1363 1363
Size: 4343
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BmtI BsaXI BsgI BsiWI
BsmI BstZ17I ClaI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI