Restriction map for
pF1KB0423
(Circular) (Six-base) MAPSORT of: pF1KB0423.seq Check: 2871 from: 1 to: 4535
pF1KB0423 4535 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AccI GT'mk_AC
Cuts at: 243 1573 243
Size: 1330 3205
Acc65I G'GTAC_C
Cuts at: 1551 1551
Size: 4535
AclI AA'CG_TT
Cuts at: 4375 4375
Size: 4535
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1114 2360 2792 3539 3807 1114
Size: 1246 432 747 268 1842
Fragments arranged by size:
1842 1246 747 432 268
AflIII A'CryG_T
Cuts at: 1887 2991 3968 1887
Size: 1104 977 2454
Fragments arranged by size:
2454 1104 977
AgeI A'CCGG_T
Cuts at: 2923 2923
Size: 4535
AlwNI CAG_nnn'CTG
Cuts at: 604 1840 3407 604
Size: 1236 1567 1732
Fragments arranged by size:
1732 1567 1236
ApaLI G'TGCA_C
Cuts at: 3305 3305
Size: 4535
ApoI r'AATT_y
Cuts at: 141 1539 3888 4144 141
Size: 1398 2349 256 532
Fragments arranged by size:
2349 1398 532 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4535
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4535
AvaI C'yCGr_G
Cuts at: 407 1555 407
Size: 1148 3387
BamHI G'GATC_C
Cuts at: 720 892 1560 720
Size: 172 668 3695
Fragments arranged by size:
3695 668 172
BanI G'GyrC_C
Cuts at: 305 1551 1965 2213 2248 305
Size: 1246 414 248 35 2592
Fragments arranged by size:
2592 1246 414 248 35
BanII G_rGCy'C
Cuts at: 1352 1549 2579 1352
Size: 197 1030 3308
Fragments arranged by size:
3308 1030 197
BbeI G_GCGC'C
Cuts at: 1969 2217 1969
Size: 248 4287
BbsI GAAGACnn'nnnn_
Cuts at: 551 1375 551
Size: 824 3711
BciVI GTATCCnnnnn_n'
Cuts at: 2433 3194 4535 2433
Size: 761 1341 2433
Fragments arranged by size:
2433 1341 761
BclI T'GATC_A
Cuts at: 2055 2055
Size: 4535
BfrBI ATG'CAT
Cuts at: 859 859
Size: 4535
BglII A'GATC_T
Cuts at: 2050 4087 2050
Size: 2037 2498
BlpI GC'TnA_GC
Cuts at: 1647 1647
Size: 4535
Bme1580I G_kGCm'C
Cuts at: 2160 2253 3309 2160
Size: 93 1056 3386
Fragments arranged by size:
3386 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1747 1828 2163 4307 1747
Size: 81 335 2144 1975
Fragments arranged by size:
2144 1975 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1014 1044 1266 2902 1014
Size: 30 222 1636 2647
Fragments arranged by size:
2647 1636 222 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 668 984 668
Size: 316 4219
Bpu10I CC'TnA_GC
Cuts at: 827 827
Size: 4535
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1711 2590 3082 3380 1711
Size: 879 492 298 2866
Fragments arranged by size:
2866 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4239 4239
Size: 4535
BsaAI yAC'GTr
Cuts at: 1888 1888
Size: 4535
BsaBI GATnn'nnATC
Cuts at: 6 2049 4086 6
Size: 2043 2037 455
Fragments arranged by size:
2043 2037 455
BsaHI Gr'CG_yC
Cuts at: 1966 2214 2916 4412 1966
Size: 248 702 1496 2089
Fragments arranged by size:
2089 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1103 1724 2245 2923 3197 3344 3755 1103
Size: 621 521 678 274 147 411 1883
Fragments arranged by size:
1883 678 621 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 298 328 871 901 996 1026 298
Size: 30 543 30 95 30 3807
Fragments arranged by size:
3807 543 95 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 227 589 1020 227
Size: 362 431 3742
Fragments arranged by size:
3742 431 362
BseYI C'CCAG_C
Cuts at: 1960 2718 3295 3791 1960
Size: 758 577 496 2704
Fragments arranged by size:
2704 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 989 989
Size: 4535
BsiEI CG_ry'CG
Cuts at: 104 2077 2123 3331 104
Size: 1973 46 1208 1308
Fragments arranged by size:
1973 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 203 884 1274 1352 1549 2327 2517 3309 203
Size: 681 390 78 197 778 190 792 1429
Fragments arranged by size:
1429 792 778 681 390 197 190 78
BsmI GAATG_Cn'
Cuts at: 745 745
Size: 4535
Bsp1286I G_dGCh'C
Cuts at: 203 884 1274 1352 1549 2160 2253 2327 2517
Size: 681 390 78 197 611 93 74 190
Cuts at: 2517 2579 3309 203
Size: 62 730 1429
Fragments arranged by size:
1429 730 681 611 390 197 190 93
78 74 62
BspEI T'CCGG_A
Cuts at: 3755 3755
Size: 4535
BspHI T'CATG_A
Cuts at: 414 3711 414
Size: 3297 1238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1585 1799 2101 2482 1585
Size: 214 302 381 3638
Fragments arranged by size:
3638 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2827 2881 4365 4530 50
Size: 2777 54 1484 165 55
Fragments arranged by size:
2777 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2447 2447
Size: 4535
BsrFI r'CCGG_y
Cuts at: 2533 2714 2923 2533
Size: 181 209 4145
Fragments arranged by size:
4145 209 181
BssSI C'ACGA_G
Cuts at: 2806 3164 2806
Size: 358 4177
BstAPI GCAn_nnn'nTGC
Cuts at: 1912 1912
Size: 4535
BstBI TT'CG_AA
Cuts at: 185 2896 185
Size: 2711 1824
BstXI CCAn_nnnn'nTGG
Cuts at: 392 436 469 775 951 392
Size: 44 33 306 176 3976
Fragments arranged by size:
3976 306 176 44 33
BstYI r'GATC_y
Cuts at: 1 720 760 892 1560 2027 2050 2385 2631
Size: 719 40 132 668 467 23 335 246
Cuts at: 2631 3643 3729 3741 4087 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 719 668 467 449 346 335 246
132 86 40 23 12
BtgI C'CryG_G
Cuts at: 107 794 107
Size: 687 3848
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 442 2485 2666 2829 442
Size: 2043 181 163 2148
Fragments arranged by size:
2148 2043 181 163
BtsI GCAGTG_nn'
Cuts at: 1506 1778 1506
Size: 272 4263
DraI TTT'AAA
Cuts at: 1534 1534
Size: 4535
DrdI GACnn_nn'nnGTC
Cuts at: 346 2241 3099 346
Size: 1895 858 1782
Fragments arranged by size:
1895 1782 858
EaeI y'GGCC_r
Cuts at: 2120 2294 2685 2712 4057 2120
Size: 174 391 27 1345 2598
Fragments arranged by size:
2598 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2120 2120
Size: 4535
EarI CTCTTCn'nnn_
Cuts at: 408 566 1066 2558 2768 408
Size: 158 500 1492 210 2175
Fragments arranged by size:
2175 1492 500 210 158
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 313 2743 3053 3199 4345 313
Size: 2430 310 146 1146 503
Fragments arranged by size:
2430 1146 503 310 146
EcoICRI GAG'CTC
Cuts at: 1350 1547 1350
Size: 197 4338
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 668 984 1114 2360 2792 3539 3807 668
Size: 316 130 1246 432 747 268 1396
Fragments arranged by size:
1396 1246 747 432 316 268 130
EcoNI CCTnn'n_nnAGG
Cuts at: 1339 1339
Size: 4535
EcoO109I rG'GnC_Cy
Cuts at: 1674 1674
Size: 4535
EcoRI G'AATT_C
Cuts at: 1539 1539
Size: 4535
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1425 2554 1425
Size: 1129 3406
FspI TGC'GCA
Cuts at: 2316 2316
Size: 4535
HaeII r_GCGC'y
Cuts at: 1969 2217 3239 4218 1969
Size: 248 1022 979 2286
Fragments arranged by size:
2286 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 871 903 2377 2407 2409 2439 871
Size: 32 1474 30 2 30 2967
Fragments arranged by size:
2967 1474 32 30 30 2
HincII GTy'rAC
Cuts at: 244 1049 1574 244
Size: 805 525 3205
Fragments arranged by size:
3205 805 525
KasI G'GCGC_C
Cuts at: 1965 2213 1965
Size: 248 4287
KpnI G_GTAC'C
Cuts at: 1555 1555
Size: 4535
MfeI C'AATT_G
Cuts at: 1016 1016
Size: 4535
MluI A'CGCG_T
Cuts at: 3968 3968
Size: 4535
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3206 3390 3206
Size: 184 4351
MscI TGG'CCA
Cuts at: 2296 2296
Size: 4535
MslI CAynn'nnrTG
Cuts at: 467 773 1208 2524 2651 467
Size: 306 435 1316 127 2351
Fragments arranged by size:
2351 1316 435 306 127
MspA1I CmG'CkG
Cuts at: 1380 1646 1960 2320 3333 3578 1380
Size: 266 314 360 1013 245 2337
Fragments arranged by size:
2337 1013 360 314 266 245
NaeI GCC'GGC
Cuts at: 2716 2716
Size: 4535
NarI GG'CG_CC
Cuts at: 1966 2214 1966
Size: 248 4287
NcoI C'CATG_G
Cuts at: 107 107
Size: 4535
NdeI CA'TA_TG
Cuts at: 363 363
Size: 4535
NgoMIV G'CCGG_C
Cuts at: 2714 2714
Size: 4535
NsiI A_TGCA'T
Cuts at: 861 861
Size: 4535
NspI r_CATG'y
Cuts at: 1588 2088 2995 1588
Size: 500 907 3128
Fragments arranged by size:
3128 907 500
PciI A'CATG_T
Cuts at: 2991 2991
Size: 4535
PflMI CCAn_nnn'nTGG
Cuts at: 604 604
Size: 4535
PfoI T'CCnGG_A
Cuts at: 484 484
Size: 4535
PmeI GTTT'AAAC
Cuts at: 1534 1534
Size: 4535
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1410 1442 3674 3706 1410
Size: 32 2232 32 2239
Fragments arranged by size:
2239 2232 32 32
PstI C_TGCA'G
Cuts at: 294 1582 2267 294
Size: 1288 685 2562
Fragments arranged by size:
2562 1288 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4535
PvuII CAG'CTG
Cuts at: 1380 1960 2320 1380
Size: 580 360 3595
Fragments arranged by size:
3595 580 360
RsrII CG'GwC_CG
Cuts at: 2730 2730
Size: 4535
SacI G_AGCT'C
Cuts at: 1352 1549 1352
Size: 197 4338
SalI G'TCGA_C
Cuts at: 242 1572 242
Size: 1330 3205
SapI GCTCTTCn'nnn_
Cuts at: 408 2558 2768 408
Size: 2150 210 2175
Fragments arranged by size:
2175 2150 210
SbfI CC_TGCA'GG
Cuts at: 1582 1582
Size: 4535
ScaI AGT'ACT
Cuts at: 4052 4052
Size: 4535
SexAI A'CCwGG_T
Cuts at: 818 818
Size: 4535
SfcI C'TryA_G
Cuts at: 33 290 1226 1578 2263 3256 3447 33
Size: 257 936 352 685 993 191 1121
Fragments arranged by size:
1121 993 936 685 352 257 191
SfoI GGC'GCC
Cuts at: 1967 2215 1967
Size: 248 4287
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4535
SmaI CCC'GGG
Cuts at: 1557 1557
Size: 4535
SmlI C'TyrA_G
Cuts at: 1690 2605 3097 3359 1690
Size: 915 492 262 2866
Fragments arranged by size:
2866 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4535
SphI G_CATG'C
Cuts at: 1588 2088 1588
Size: 500 4035
StyI C'CwwG_G
Cuts at: 107 1444 1669 2023 107
Size: 1337 225 354 2619
Fragments arranged by size:
2619 1337 354 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2118 2251 2710 2919 2118
Size: 133 459 209 3734
Fragments arranged by size:
3734 459 209 133
TatI w'GTAC_w
Cuts at: 4050 4050
Size: 4535
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 298 330 480 512 996 1028 4052 4084 298
Size: 32 150 32 484 32 3024 32 749
Fragments arranged by size:
3024 749 484 150 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2332 2332
Size: 4535
XbaI T'CTAG_A
Cuts at: 1260 1566 1260
Size: 306 4229
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2030 2030
Size: 4535
XmaI C'CCGG_G
Cuts at: 1555 1555
Size: 4535
XmnI GAAnn'nnTTC
Cuts at: 1408 1408
Size: 4535
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
BmtI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
NheI NotI NruI PacI PasI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI