Restriction map for
pF1KB0428
(Circular) (Six-base) MAPSORT of: pF1KB0428.seq Check: 3921 from: 1 to: 5669
pF1KB0428 5669 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 829 829
Size: 5669
AccI GT'mk_AC
Cuts at: 2707 2707
Size: 5669
Acc65I G'GTAC_C
Cuts at: 2685 2685
Size: 5669
AclI AA'CG_TT
Cuts at: 5509 5509
Size: 5669
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1205 1688 2151 2571 3494 3926 4673 4941 1205
Size: 483 463 420 923 432 747 268 1933
Fragments arranged by size:
1933 923 747 483 463 432 420 268
AflII C'TTAA_G
Cuts at: 2010 2010
Size: 5669
AflIII A'CryG_T
Cuts at: 3021 4125 5102 3021
Size: 1104 977 3588
Fragments arranged by size:
3588 1104 977
AgeI A'CCGG_T
Cuts at: 4057 4057
Size: 5669
AlwNI CAG_nnn'CTG
Cuts at: 306 436 920 1444 1756 2974 4541 306
Size: 130 484 524 312 1218 1567 1434
Fragments arranged by size:
1567 1434 1218 524 484 312 130
ApaLI G'TGCA_C
Cuts at: 844 2177 2441 2525 4439 844
Size: 1333 264 84 1914 2074
Fragments arranged by size:
2074 1914 1333 264 84
ApoI r'AATT_y
Cuts at: 1895 2265 2673 5022 5278 1895
Size: 370 408 2349 256 2286
Fragments arranged by size:
2349 2286 408 370 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5669
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5669
AvaI C'yCGr_G
Cuts at: 2689 2689
Size: 5669
BamHI G'GATC_C
Cuts at: 686 2694 686
Size: 2008 3661
BanI G'GyrC_C
Cuts at: 1545 2685 3099 3347 3382 1545
Size: 1140 414 248 35 3832
Fragments arranged by size:
3832 1140 414 248 35
BanII G_rGCy'C
Cuts at: 706 2347 2683 3713 706
Size: 1641 336 1030 2662
Fragments arranged by size:
2662 1641 1030 336
BbeI G_GCGC'C
Cuts at: 3103 3351 3103
Size: 248 5421
BbsI GAAGACnn'nnnn_
Cuts at: 417 1956 417
Size: 1539 4130
BbvCI CC'TCA_GC
Cuts at: 1685 1685
Size: 5669
BciVI GTATCCnnnnn_n'
Cuts at: 1728 2127 2447 3567 4328 5669 1728
Size: 399 320 1120 761 1341 1728
Fragments arranged by size:
1728 1341 1120 761 399 320
BclI T'GATC_A
Cuts at: 3189 3189
Size: 5669
BfrBI ATG'CAT
Cuts at: 2109 2109
Size: 5669
BglI GCCn_nnn'nGGC
Cuts at: 112 1166 112
Size: 1054 4615
BglII A'GATC_T
Cuts at: 418 693 1882 3184 5221 418
Size: 275 1189 1302 2037 866
Fragments arranged by size:
2037 1302 1189 866 275
BlpI GC'TnA_GC
Cuts at: 956 2781 956
Size: 1825 3844
Bme1580I G_kGCm'C
Cuts at: 848 2181 2445 2529 3294 3387 4443 848
Size: 1333 264 84 765 93 1056 2074
Fragments arranged by size:
2074 1333 1056 765 264 93 84
BmrI ACTGGGnnnn_n'
Cuts at: 858 1118 1347 1380 2881 2962 3297 5441 858
Size: 260 229 33 1501 81 335 2144 1086
Fragments arranged by size:
2144 1501 1086 335 260 229 81 33
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4036 4036
Size: 5669
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 645 834 1006 1757 2231 645
Size: 189 172 751 474 4083
Fragments arranged by size:
4083 751 474 189 172
Bpu10I CC'TnA_GC
Cuts at: 247 964 1106 1241 1685 247
Size: 717 142 135 444 4231
Fragments arranged by size:
4231 717 444 142 135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2331 2845 3724 4216 4514 2331
Size: 514 879 492 298 3486
Fragments arranged by size:
3486 879 514 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 145 663 856 1024 1057 1345 1375 1546 2028
Size: 518 193 168 33 288 30 171 482
Cuts at: 2028 2046 2463 5373 145
Size: 18 417 2910 441
Fragments arranged by size:
2910 518 482 441 417 288 193 171
168 33 30 18
BsaAI yAC'GTr
Cuts at: 2524 3022 2524
Size: 498 5171
BsaBI GATnn'nnATC
Cuts at: 6 3183 5220 6
Size: 3177 2037 455
Fragments arranged by size:
3177 2037 455
BsaHI Gr'CG_yC
Cuts at: 3100 3348 4050 5546 3100
Size: 248 702 1496 3223
Fragments arranged by size:
3223 1496 702 248
BsaWI w'CCGG_w
Cuts at: 649 983 1403 2195 2858 3379 4057 4331 4478
Size: 334 420 792 663 521 678 274 147
Cuts at: 4478 4889 649
Size: 411 1429
Fragments arranged by size:
1429 792 678 663 521 420 411 334
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1755 1785 1979 2009 1755
Size: 30 194 30 5415
Fragments arranged by size:
5415 194 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 382 865 1231 382
Size: 483 366 4820
Fragments arranged by size:
4820 483 366
BseYI C'CCAG_C
Cuts at: 196 527 699 794 3094 3852 4429 4925 196
Size: 331 172 95 2300 758 577 496 940
Fragments arranged by size:
2300 940 758 577 496 331 172 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1286 1286
Size: 5669
BsiEI CG_ry'CG
Cuts at: 104 3211 3257 4465 104
Size: 3107 46 1208 1308
Fragments arranged by size:
3107 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 848 1107 1773 2181 2347 2445 2529 2683 3461
Size: 259 666 408 166 98 84 154 778
Cuts at: 3461 3651 4443 848
Size: 190 792 2074
Fragments arranged by size:
2074 792 778 666 408 259 190 166
154 98 84
Bsp1286I G_dGCh'C
Cuts at: 706 848 1107 1773 2181 2347 2445 2529 2683
Size: 142 259 666 408 166 98 84 154
Cuts at: 2683 3294 3387 3461 3651 3713 4443 706
Size: 611 93 74 190 62 730 1932
Fragments arranged by size:
1932 730 666 611 408 259 190 166
154 142 98 93 84 74 62
BspEI T'CCGG_A
Cuts at: 983 1403 4889 983
Size: 420 3486 1763
Fragments arranged by size:
3486 1763 420
BspHI T'CATG_A
Cuts at: 2568 4845 2568
Size: 2277 3392
BspMI ACCTGCnnnn'nnnn_
Cuts at: 829 1075 2719 2933 3235 3616 829
Size: 246 1644 214 302 381 2882
Fragments arranged by size:
2882 1644 381 302 246 214
BsrBI CCG'CTC
Cuts at: 50 629 960 1049 3961 4015 5499 5664 50
Size: 579 331 89 2912 54 1484 165 55
Fragments arranged by size:
2912 1484 579 331 165 89 55 54
BsrDI GCAATG_nn'
Cuts at: 1112 1932 3581 1112
Size: 820 1649 3200
Fragments arranged by size:
3200 1649 820
BsrFI r'CCGG_y
Cuts at: 3667 3848 4057 3667
Size: 181 209 5279
Fragments arranged by size:
5279 209 181
BsrGI T'GTAC_A
Cuts at: 1332 1332
Size: 5669
BssSI C'ACGA_G
Cuts at: 382 3940 4298 382
Size: 3558 358 1753
Fragments arranged by size:
3558 1753 358
BstAPI GCAn_nnn'nTGC
Cuts at: 453 3046 453
Size: 2593 3076
BstBI TT'CG_AA
Cuts at: 4030 4030
Size: 5669
BstXI CCAn_nnnn'nTGG
Cuts at: 943 1432 943
Size: 489 5180
BstYI r'GATC_y
Cuts at: 1 326 418 686 693 1266 1882 2694 3161
Size: 325 92 268 7 573 616 812 467
Cuts at: 3161 3184 3519 3765 4777 4863 4875 5221 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 812 616 573 467 449 346 335
325 268 246 92 86 23 12 7
Bsu36I CC'TnA_GG
Cuts at: 1968 1968
Size: 5669
BtgI C'CryG_G
Cuts at: 202 682 1794 202
Size: 480 1112 4077
Fragments arranged by size:
4077 1112 480
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1187 3619 3800 3963 1187
Size: 2432 181 163 2893
Fragments arranged by size:
2893 2432 181 163
BtsI GCAGTG_nn'
Cuts at: 380 1445 2912 380
Size: 1065 1467 3137
Fragments arranged by size:
3137 1467 1065
DraI TTT'AAA
Cuts at: 1503 2668 1503
Size: 1165 4504
DraIII CAC_nnn'GTG
Cuts at: 2127 2127
Size: 5669
DrdI GACnn_nn'nnGTC
Cuts at: 3375 4233 3375
Size: 858 4811
EaeI y'GGCC_r
Cuts at: 113 1050 1539 1620 3254 3428 3819 3846 5191
Size: 937 489 81 1634 174 391 27 1345
Cuts at: 5191 113
Size: 591
Fragments arranged by size:
1634 1345 937 591 489 391 174 81
27
EagI C'GGCC_G
Cuts at: 3254 3254
Size: 5669
EarI CTCTTCn'nnn_
Cuts at: 854 1021 1076 1181 1223 1343 1565 1622 1724
Size: 167 55 105 42 120 222 57 102
Cuts at: 1724 1999 2396 3692 3902 854
Size: 275 397 1296 210 2621
Fragments arranged by size:
2621 1296 397 275 222 210 167 120
105 102 57 55 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3877 4187 4333 5479 3877
Size: 310 146 1146 4067
Fragments arranged by size:
4067 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2345 2681 2345
Size: 336 5333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 645 834 1006 1205 1688 1757 2151 2231 2571
Size: 189 172 199 483 69 394 80 340
Cuts at: 2571 3494 3926 4673 4941 645
Size: 923 432 747 268 1373
Fragments arranged by size:
1373 923 747 483 432 394 340 268
199 189 172 80 69
EcoO109I rG'GnC_Cy
Cuts at: 1287 1475 2808 1287
Size: 188 1333 4148
Fragments arranged by size:
4148 1333 188
EcoRI G'AATT_C
Cuts at: 2673 2673
Size: 5669
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3688 3688
Size: 5669
FspI TGC'GCA
Cuts at: 262 3450 262
Size: 3188 2481
FspAI rTGC'GCAy
Cuts at: 262 262
Size: 5669
HaeII r_GCGC'y
Cuts at: 3103 3351 4373 5352 3103
Size: 248 1022 979 3420
Fragments arranged by size:
3420 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 410 442 678 710 1089 1121 1279 1311 1927
Size: 32 236 32 379 32 158 32 616
Cuts at: 1927 1959 3511 3541 3543 3573 410
Size: 32 1552 30 2 30 2506
Fragments arranged by size:
2506 1552 616 379 236 158 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2029 2708 2029
Size: 679 4990
HindIII A'AGCT_T
Cuts at: 2082 2163 2415 2638 2082
Size: 81 252 223 5113
Fragments arranged by size:
5113 252 223 81
KasI G'GCGC_C
Cuts at: 3099 3347 3099
Size: 248 5421
KpnI G_GTAC'C
Cuts at: 2689 2689
Size: 5669
MluI A'CGCG_T
Cuts at: 5102 5102
Size: 5669
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 183 647 982 1471 2152 4340 4524 183
Size: 464 335 489 681 2188 184 1328
Fragments arranged by size:
2188 1328 681 489 464 335 184
MscI TGG'CCA
Cuts at: 115 1541 1622 3430 115
Size: 1426 81 1808 2354
Fragments arranged by size:
2354 1808 1426 81
MslI CAynn'nnrTG
Cuts at: 2142 2438 2573 3658 3785 2142
Size: 296 135 1085 127 4026
Fragments arranged by size:
4026 1085 296 135 127
MspA1I CmG'CkG
Cuts at: 204 1300 1428 2780 3094 3454 4467 4712 204
Size: 1096 128 1352 314 360 1013 245 1161
Fragments arranged by size:
1352 1161 1096 1013 360 314 245 128
NaeI GCC'GGC
Cuts at: 3850 3850
Size: 5669
NarI GG'CG_CC
Cuts at: 3100 3348 3100
Size: 248 5421
NcoI C'CATG_G
Cuts at: 682 682
Size: 5669
NgoMIV G'CCGG_C
Cuts at: 3848 3848
Size: 5669
NsiI A_TGCA'T
Cuts at: 2111 2111
Size: 5669
NspI r_CATG'y
Cuts at: 760 2722 3222 4129 760
Size: 1962 500 907 2300
Fragments arranged by size:
2300 1962 907 500
PasI CC'CwG_GG
Cuts at: 533 1750 533
Size: 1217 4452
PciI A'CATG_T
Cuts at: 4125 4125
Size: 5669
PflMI CCAn_nnn'nTGG
Cuts at: 192 361 2127 192
Size: 169 1766 3734
Fragments arranged by size:
3734 1766 169
PfoI T'CCnGG_A
Cuts at: 1997 1997
Size: 5669
PmeI GTTT'AAAC
Cuts at: 2668 2668
Size: 5669
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4808 4840 4808
Size: 32 5637
PpuMI rG'GwC_Cy
Cuts at: 1287 1475 1287
Size: 188 5481
PsiI TTA'TAA
Cuts at: 2225 2225
Size: 5669
PstI C_TGCA'G
Cuts at: 2716 3401 2716
Size: 685 4984
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5669
PvuII CAG'CTG
Cuts at: 1300 1428 3094 3454 1300
Size: 128 1666 360 3515
Fragments arranged by size:
3515 1666 360 128
RsrII CG'GwC_CG
Cuts at: 3864 3864
Size: 5669
SacI G_AGCT'C
Cuts at: 2347 2683 2347
Size: 336 5333
SacII CC_GC'GG
Cuts at: 205 205
Size: 5669
SalI G'TCGA_C
Cuts at: 2706 2706
Size: 5669
SapI GCTCTTCn'nnn_
Cuts at: 1021 1181 1999 3692 3902 1021
Size: 160 818 1693 210 2788
Fragments arranged by size:
2788 1693 818 210 160
SbfI CC_TGCA'GG
Cuts at: 2716 2716
Size: 5669
ScaI AGT'ACT
Cuts at: 5186 5186
Size: 5669
SexAI A'CCwGG_T
Cuts at: 2123 2123
Size: 5669
SfcI C'TryA_G
Cuts at: 33 1938 2712 3397 4390 4581 33
Size: 1905 774 685 993 191 1121
Fragments arranged by size:
1905 1121 993 774 685 191
SfoI GGC'GCC
Cuts at: 3101 3349 3101
Size: 248 5421
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5669
SmaI CCC'GGG
Cuts at: 2691 2691
Size: 5669
SmlI C'TyrA_G
Cuts at: 2010 2346 2824 3739 4231 4493 2010
Size: 336 478 915 492 262 3186
Fragments arranged by size:
3186 915 492 478 336 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5669
SphI G_CATG'C
Cuts at: 760 2722 3222 760
Size: 1962 500 3207
Fragments arranged by size:
3207 1962 500
StyI C'CwwG_G
Cuts at: 266 682 2660 2803 3157 266
Size: 416 1978 143 354 2778
Fragments arranged by size:
2778 1978 416 354 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 414 786 1070 1626 2290 2552 3252 3385 3844
Size: 372 284 556 664 262 700 133 459
Cuts at: 3844 4053 414
Size: 209 2030
Fragments arranged by size:
2030 700 664 556 459 372 284 262
209 133
TatI w'GTAC_w
Cuts at: 1332 1854 5184 1332
Size: 522 3330 1817
Fragments arranged by size:
3330 1817 522
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5186 5218 5186
Size: 32 5637
Tth111I GACn'n_nGTC
Cuts at: 2649 3466 2649
Size: 817 4852
XbaI T'CTAG_A
Cuts at: 1269 2700 1269
Size: 1431 4238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 193 1131 1792 3164 193
Size: 938 661 1372 2698
Fragments arranged by size:
2698 1372 938 661
XmaI C'CCGG_G
Cuts at: 2689 2689
Size: 5669
XmnI GAAnn'nnTTC
Cuts at: 2164 2332 2639 2164
Size: 168 307 5194
Fragments arranged by size:
5194 307 168
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BmgBI BmtI BsiWI BsmI BsmBI
BssHII BstEII BstZ17I ClaI CspCI EcoNI EcoRV FseI
HpaI MfeI NdeI NheI NotI NruI PacI PmlI
PshAI PspOMI PspXI PsrI SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI