Restriction map for
pF1KB0430
(Circular) (Six-base) MAPSORT of: pF1KB0430.seq Check: 4715 from: 1 to: 5669
pF1KB0430 5669 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 725 725
Size: 5669
AccI GT'mk_AC
Cuts at: 1173 2707 1173
Size: 1534 4135
Acc65I G'GTAC_C
Cuts at: 2325 2685 2325
Size: 360 5309
AclI AA'CG_TT
Cuts at: 5509 5509
Size: 5669
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1059 1083 1265 3494 3926 4673 4941 1059
Size: 24 182 2229 432 747 268 1787
Fragments arranged by size:
2229 1787 747 432 268 182 24
AflIII A'CryG_T
Cuts at: 2363 3021 4125 5102 2363
Size: 658 1104 977 2930
Fragments arranged by size:
2930 1104 977 658
AgeI A'CCGG_T
Cuts at: 4057 4057
Size: 5669
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2243 2243
Size: 5669
AlwNI CAG_nnn'CTG
Cuts at: 834 1792 1975 2256 2310 2974 4541 834
Size: 958 183 281 54 664 1567 1962
Fragments arranged by size:
1962 1567 958 664 281 183 54
ApaI G_GGCC'C
Cuts at: 329 1120 329
Size: 791 4878
ApaLI G'TGCA_C
Cuts at: 2479 4439 2479
Size: 1960 3709
ApoI r'AATT_y
Cuts at: 429 1795 2673 5022 5278 429
Size: 1366 878 2349 256 820
Fragments arranged by size:
2349 1366 878 820 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5669
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5669
AvaI C'yCGr_G
Cuts at: 201 2601 2689 201
Size: 2400 88 3181
Fragments arranged by size:
3181 2400 88
AvrII C'CTAG_G
Cuts at: 1606 1606
Size: 5669
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2043 2076 2309 2342 2043
Size: 33 233 33 5370
Fragments arranged by size:
5370 233 33 33
BamHI G'GATC_C
Cuts at: 2694 2694
Size: 5669
BanI G'GyrC_C
Cuts at: 1125 1317 1362 2325 2644 2685 3099 3347 3382
Size: 192 45 963 319 41 414 248 35
Cuts at: 3382 1125
Size: 3412
Fragments arranged by size:
3412 963 414 319 248 192 45 41
35
BanII G_rGCy'C
Cuts at: 329 529 1067 1120 1334 2132 2279 2683 3713
Size: 200 538 53 214 798 147 404 1030
Cuts at: 3713 329
Size: 2285
Fragments arranged by size:
2285 1030 798 538 404 214 200 147
53
BbeI G_GCGC'C
Cuts at: 3103 3351 3103
Size: 248 5421
BbsI GAAGACnn'nnnn_
Cuts at: 1253 2197 1253
Size: 944 4725
BbvCI CC'TCA_GC
Cuts at: 1957 2017 1957
Size: 60 5609
BciVI GTATCCnnnnn_n'
Cuts at: 1755 2599 3567 4328 5669 1755
Size: 844 968 761 1341 1755
Fragments arranged by size:
1755 1341 968 844 761
BclI T'GATC_A
Cuts at: 3189 3189
Size: 5669
BglI GCCn_nnn'nGGC
Cuts at: 300 822 1867 2573 2629 300
Size: 522 1045 706 56 3340
Fragments arranged by size:
3340 1045 706 522 56
BglII A'GATC_T
Cuts at: 3184 5221 3184
Size: 2037 3632
BlpI GC'TnA_GC
Cuts at: 1712 2781 1712
Size: 1069 4600
Bme1580I G_kGCm'C
Cuts at: 329 1120 1130 2151 2483 2578 2647 3294 3387
Size: 791 10 1021 332 95 69 647 93
Cuts at: 3387 4443 329
Size: 1056 1555
Fragments arranged by size:
1555 1056 1021 791 647 332 95 93
69 10
BmrI ACTGGGnnnn_n'
Cuts at: 2881 2962 3297 5441 2881
Size: 81 335 2144 3109
Fragments arranged by size:
3109 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1926 4036 1926
Size: 2110 3559
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 507 622 759 1194 1348 1750 1835 507
Size: 115 137 435 154 402 85 4341
Fragments arranged by size:
4341 435 402 154 137 115 85
Bpu10I CC'TnA_GC
Cuts at: 719 1635 1957 2017 2615 719
Size: 916 322 60 598 3773
Fragments arranged by size:
3773 916 598 322 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 405 427 2146 2845 3724 4216 4514 405
Size: 22 1719 699 879 492 298 1560
Fragments arranged by size:
1719 1560 879 699 492 298 22
BsaI GGTCTCn'nnnn_
Cuts at: 5373 5373
Size: 5669
BsaAI yAC'GTr
Cuts at: 3022 3022
Size: 5669
BsaBI GATnn'nnATC
Cuts at: 6 3183 5220 6
Size: 3177 2037 455
Fragments arranged by size:
3177 2037 455
BsaHI Gr'CG_yC
Cuts at: 967 3100 3348 4050 5546 967
Size: 2133 248 702 1496 1090
Fragments arranged by size:
2133 1496 1090 702 248
BsaWI w'CCGG_w
Cuts at: 578 1760 2858 3379 4057 4331 4478 4889 578
Size: 1182 1098 521 678 274 147 411 1358
Fragments arranged by size:
1358 1182 1098 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 742 772 1413 1443 1945 1975 742
Size: 30 641 30 502 30 4436
Fragments arranged by size:
4436 641 502 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 823 826 1747 2257 2287 823
Size: 3 921 510 30 4205
Fragments arranged by size:
4205 921 510 30 3
BseYI C'CCAG_C
Cuts at: 163 691 795 1069 1495 1500 1917 1924 2106
Size: 528 104 274 426 5 417 7 182
Cuts at: 2106 2150 2155 2249 2571 3094 3852 4429 4925
Size: 44 5 94 322 523 758 577 496
Cuts at: 4925 163
Size: 907
Fragments arranged by size:
907 758 577 528 523 496 426 417
322 274 182 104 94 44 7 5
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1788 2380 1788
Size: 592 5077
BsiEI CG_ry'CG
Cuts at: 104 3211 3257 4465 104
Size: 3107 46 1208 1308
Fragments arranged by size:
3107 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2140 2279 2483 2683 3461 3651 4443 2140
Size: 139 204 200 778 190 792 3366
Fragments arranged by size:
3366 792 778 204 200 190 139
BsiWI C'GTAC_G
Cuts at: 2389 2389
Size: 5669
Bsp1286I G_dGCh'C
Cuts at: 329 529 1067 1120 1130 1334 2132 2140 2151
Size: 200 538 53 10 204 798 8 11
Cuts at: 2151 2279 2483 2578 2647 2683 3294 3387 3461
Size: 128 204 95 69 36 611 93 74
Cuts at: 3461 3651 3713 4443 329
Size: 190 62 730 1555
Fragments arranged by size:
1555 798 730 611 538 204 204 200
190 128 95 93 74 69 62 53
36 11 10 8
BspEI T'CCGG_A
Cuts at: 4889 4889
Size: 5669
BspHI T'CATG_A
Cuts at: 4845 4845
Size: 5669
BspMI ACCTGCnnnn'nnnn_
Cuts at: 115 135 231 725 2719 2933 3235 3616 115
Size: 20 96 494 1994 214 302 381 2168
Fragments arranged by size:
2168 1994 494 381 302 214 96 20
BsrBI CCG'CTC
Cuts at: 50 236 1905 2536 3961 4015 5499 5664 50
Size: 186 1669 631 1425 54 1484 165 55
Fragments arranged by size:
1669 1484 1425 631 186 165 55 54
BsrDI GCAATG_nn'
Cuts at: 760 945 3581 760
Size: 185 2636 2848
Fragments arranged by size:
2848 2636 185
BsrFI r'CCGG_y
Cuts at: 112 2054 3667 3848 4057 112
Size: 1942 1613 181 209 1724
Fragments arranged by size:
1942 1724 1613 209 181
BssHII G'CGCG_C
Cuts at: 116 116
Size: 5669
BssSI C'ACGA_G
Cuts at: 2131 3940 4298 2131
Size: 1809 358 3502
Fragments arranged by size:
3502 1809 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2310 3046 2310
Size: 736 4933
BstBI TT'CG_AA
Cuts at: 4030 4030
Size: 5669
BstXI CCAn_nnnn'nTGG
Cuts at: 1159 1159
Size: 5669
BstYI r'GATC_y
Cuts at: 1 464 2694 3161 3184 3519 3765 4777 4863
Size: 463 2230 467 23 335 246 1012 86
Cuts at: 4863 4875 5221 1
Size: 12 346 449
Fragments arranged by size:
2230 1012 467 463 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 563 920 563
Size: 357 5312
BtgI C'CryG_G
Cuts at: 107 134 151 320 593 1082 1358 2075 2468
Size: 27 17 169 273 489 276 717 393
Cuts at: 2468 107
Size: 3308
Fragments arranged by size:
3308 717 489 393 276 273 169 27
17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3619 3800 3963 3619
Size: 181 163 5325
Fragments arranged by size:
5325 181 163
BtsI GCAGTG_nn'
Cuts at: 970 1110 1370 1646 1664 1938 2912 970
Size: 140 260 276 18 274 974 3727
Fragments arranged by size:
3727 974 276 274 260 140 18
DraI TTT'AAA
Cuts at: 2668 2668
Size: 5669
DraIII CAC_nnn'GTG
Cuts at: 2146 2146
Size: 5669
DrdI GACnn_nn'nnGTC
Cuts at: 1169 3375 4233 1169
Size: 2206 858 2605
Fragments arranged by size:
2605 2206 858
EaeI y'GGCC_r
Cuts at: 110 131 739 1142 1843 1859 2542 3254 3428
Size: 21 608 403 701 16 683 712 174
Cuts at: 3428 3819 3846 5191 110
Size: 391 27 1345 588
Fragments arranged by size:
1345 712 701 683 608 588 403 391
174 27 21 16
EagI C'GGCC_G
Cuts at: 3254 3254
Size: 5669
EarI CTCTTCn'nnn_
Cuts at: 379 517 775 880 1102 1382 1402 3692 3902
Size: 138 258 105 222 280 20 2290 210
Cuts at: 3902 379
Size: 2146
Fragments arranged by size:
2290 2146 280 258 222 210 138 105
20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 457 742 2479 3877 4187 4333 5479 457
Size: 285 1737 1398 310 146 1146 647
Fragments arranged by size:
1737 1398 1146 647 310 285 146
EcoICRI GAG'CTC
Cuts at: 2277 2681 2277
Size: 404 5265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 507 622 759 1059 1083 1194 1265 1348 1750
Size: 115 137 300 24 111 71 83 402
Cuts at: 1750 1835 3494 3926 4673 4941 507
Size: 85 1659 432 747 268 1235
Fragments arranged by size:
1659 1235 747 432 402 300 268 137
115 111 85 83 71 24
EcoNI CCTnn'n_nnAGG
Cuts at: 1436 1436
Size: 5669
EcoO109I rG'GnC_Cy
Cuts at: 147 198 325 326 491 559 801 1109 1772
Size: 51 127 1 165 68 242 308 663
Cuts at: 1772 1880 1953 1972 2808 147
Size: 108 73 19 836 3008
Fragments arranged by size:
3008 836 663 308 242 165 127 108
73 68 51 19 1
EcoRI G'AATT_C
Cuts at: 1795 2673 1795
Size: 878 4791
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2109 3688 2109
Size: 1579 4090
FspI TGC'GCA
Cuts at: 575 3450 575
Size: 2875 2794
FspAI rTGC'GCAy
Cuts at: 575 575
Size: 5669
HaeII r_GCGC'y
Cuts at: 1217 1924 2094 3103 3351 4373 5352 1217
Size: 707 170 1009 248 1022 979 1534
Fragments arranged by size:
1534 1022 1009 979 707 248 170
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 163 175 195 207 449 481 1945 1977 3511
Size: 12 20 12 242 32 1464 32 1534
Cuts at: 3511 3541 3543 3573 163
Size: 30 2 30 2259
Fragments arranged by size:
2259 1534 1464 242 32 32 30 30
20 12 12 2
HincII GTy'rAC
Cuts at: 1089 2708 1089
Size: 1619 4050
KasI G'GCGC_C
Cuts at: 3099 3347 3099
Size: 248 5421
KpnI G_GTAC'C
Cuts at: 2329 2689 2329
Size: 360 5309
MfeI C'AATT_G
Cuts at: 843 843
Size: 5669
MluI A'CGCG_T
Cuts at: 5102 5102
Size: 5669
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 356 881 4340 4524 356
Size: 525 3459 184 1501
Fragments arranged by size:
3459 1501 525 184
MscI TGG'CCA
Cuts at: 3430 3430
Size: 5669
MslI CAynn'nnrTG
Cuts at: 631 2209 3658 3785 631
Size: 1578 1449 127 2515
Fragments arranged by size:
2515 1578 1449 127
MspA1I CmG'CkG
Cuts at: 136 153 254 912 1190 1452 1698 1928 2077
Size: 17 101 658 278 262 246 230 149
Cuts at: 2077 2197 2256 2310 2571 2780 3094 3454 4467
Size: 120 59 54 261 209 314 360 1013
Cuts at: 4467 4712 136
Size: 245 1093
Fragments arranged by size:
1093 1013 658 360 314 278 262 261
246 245 230 209 149 120 101 59
54 17
NaeI GCC'GGC
Cuts at: 114 3850 114
Size: 3736 1933
NarI GG'CG_CC
Cuts at: 3100 3348 3100
Size: 248 5421
NcoI C'CATG_G
Cuts at: 107 1358 107
Size: 1251 4418
NgoMIV G'CCGG_C
Cuts at: 112 3848 112
Size: 3736 1933
NspI r_CATG'y
Cuts at: 2367 2722 3222 4129 2367
Size: 355 500 907 3907
Fragments arranged by size:
3907 907 500 355
PasI CC'CwG_GG
Cuts at: 1113 1768 1975 1113
Size: 655 207 4807
Fragments arranged by size:
4807 655 207
PciI A'CATG_T
Cuts at: 2363 4125 2363
Size: 1762 3907
PflMI CCAn_nnn'nTGG
Cuts at: 1975 1975
Size: 5669
PfoI T'CCnGG_A
Cuts at: 599 833 599
Size: 234 5435
PmeI GTTT'AAAC
Cuts at: 2668 2668
Size: 5669
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4808 4840 4808
Size: 32 5637
PpuMI rG'GwC_Cy
Cuts at: 147 198 801 1109 1772 1953 147
Size: 51 603 308 663 181 3863
Fragments arranged by size:
3863 663 603 308 181 51
PshAI GACnn'nnGTC
Cuts at: 203 203
Size: 5669
PspOMI G'GGCC_C
Cuts at: 325 1116 325
Size: 791 4878
PstI C_TGCA'G
Cuts at: 457 557 911 977 1385 1467 2189 2716 3401
Size: 100 354 66 408 82 722 527 685
Cuts at: 3401 457
Size: 2725
Fragments arranged by size:
2725 722 685 527 408 354 100 82
66
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5669
PvuII CAG'CTG
Cuts at: 912 1190 1698 2197 2256 2310 2571 3094 3454
Size: 278 508 499 59 54 261 523 360
Cuts at: 3454 912
Size: 3127
Fragments arranged by size:
3127 523 508 499 360 278 261 59
54
RsrII CG'GwC_CG
Cuts at: 3864 3864
Size: 5669
SacI G_AGCT'C
Cuts at: 2279 2683 2279
Size: 404 5265
SacII CC_GC'GG
Cuts at: 137 154 2078 137
Size: 17 1924 3728
Fragments arranged by size:
3728 1924 17
SalI G'TCGA_C
Cuts at: 2706 2706
Size: 5669
SanDI GG'GwC_CC
Cuts at: 801 1109 1772 801
Size: 308 663 4698
Fragments arranged by size:
4698 663 308
SapI GCTCTTCn'nnn_
Cuts at: 517 775 1382 3692 3902 517
Size: 258 607 2310 210 2284
Fragments arranged by size:
2310 2284 607 258 210
SbfI CC_TGCA'GG
Cuts at: 557 2716 557
Size: 2159 3510
ScaI AGT'ACT
Cuts at: 2521 5186 2521
Size: 2665 3004
SfcI C'TryA_G
Cuts at: 33 396 453 553 907 973 1174 1381 1463
Size: 363 57 100 354 66 201 207 82
Cuts at: 1463 2185 2215 2712 3397 4390 4581 33
Size: 722 30 497 685 993 191 1121
Fragments arranged by size:
1121 993 722 685 497 363 354 207
201 191 100 82 66 57 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 1867 1867
Size: 5669
SfoI GGC'GCC
Cuts at: 3101 3349 3101
Size: 248 5421
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5669
SmaI CCC'GGG
Cuts at: 2603 2691 2603
Size: 88 5581
SmlI C'TyrA_G
Cuts at: 384 442 2161 2824 3739 4231 4493 384
Size: 58 1719 663 915 492 262 1560
Fragments arranged by size:
1719 1560 915 663 492 262 58
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5669
SphI G_CATG'C
Cuts at: 2722 3222 2722
Size: 500 5169
StuI AGG'CCT
Cuts at: 440 1325 440
Size: 885 4784
StyI C'CwwG_G
Cuts at: 107 1202 1313 1358 1535 1606 2649 2803 3157
Size: 1095 111 45 177 71 1043 154 354
Cuts at: 3157 107
Size: 2619
Fragments arranged by size:
2619 1095 1043 354 177 154 111 71
45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1383 1491 2062 2403 2611 3252 3385 3844 4053
Size: 108 571 341 208 641 133 459 209
Cuts at: 4053 1383
Size: 2999
Fragments arranged by size:
2999 641 571 459 341 209 208 133
108
TatI w'GTAC_w
Cuts at: 2201 2519 5184 2201
Size: 318 2665 2686
Fragments arranged by size:
2686 2665 318
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 721 742 753 774 1334 1366 1413 1445 1475
Size: 21 11 21 560 32 47 32 30
Cuts at: 1475 1507 1638 1670 1797 1829 5186 5218 721
Size: 32 131 32 127 32 3357 32 1172
Fragments arranged by size:
3357 1172 560 131 127 47 32 32
32 32 32 32 30 21 21 11
Tth111I GACn'n_nGTC
Cuts at: 888 2377 3466 888
Size: 1489 1089 3091
Fragments arranged by size:
3091 1489 1089
XbaI T'CTAG_A
Cuts at: 2700 2700
Size: 5669
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3164 3164
Size: 5669
XmaI C'CCGG_G
Cuts at: 2601 2689 2601
Size: 88 5581
XmnI GAAnn'nnTTC
Cuts at: 430 430
Size: 5669
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI BcgI
BfrBI BmgBI BmtI BsmI BsmBI BsrGI BstEII BstZ17I
ClaI CspCI EcoRV FseI HindIII HpaI NdeI NheI
NotI NruI NsiI PacI PmlI PsiI PspXI PsrI
SexAI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI