Restriction map for
pF1KB0434
(Circular) (Six-base) MAPSORT of: pF1KB0434.seq Check: 6505 from: 1 to: 5711
pF1KB0434 5711 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AccI GT'mk_AC
Cuts at: 2749 2749
Size: 5711
Acc65I G'GTAC_C
Cuts at: 2727 2727
Size: 5711
AclI AA'CG_TT
Cuts at: 5551 5551
Size: 5711
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 514 1511 2705 3536 3968 4715 4983 358
Size: 156 997 1194 831 432 747 268 1086
Fragments arranged by size:
1194 1086 997 831 747 432 268 156
AflII C'TTAA_G
Cuts at: 456 1621 456
Size: 1165 4546
AflIII A'CryG_T
Cuts at: 1865 2509 3063 4167 5144 1865
Size: 644 554 1104 977 2432
Fragments arranged by size:
2432 1104 977 644 554
AgeI A'CCGG_T
Cuts at: 4099 4099
Size: 5711
AlwNI CAG_nnn'CTG
Cuts at: 2683 3016 4583 2683
Size: 333 1567 3811
Fragments arranged by size:
3811 1567 333
ApaLI G'TGCA_C
Cuts at: 4481 4481
Size: 5711
ApoI r'AATT_y
Cuts at: 199 229 625 941 1056 1539 1606 2083 2352
Size: 30 396 316 115 483 67 477 269
Cuts at: 2352 2379 2573 2715 5064 5320 199
Size: 27 194 142 2349 256 590
Fragments arranged by size:
2349 590 483 477 396 316 269 256
194 142 115 67 30 27
AseI AT'TA_AT
Cuts at: 20 1327 20
Size: 1307 4404
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5711
AvaI C'yCGr_G
Cuts at: 2731 2731
Size: 5711
AvrII C'CTAG_G
Cuts at: 2547 2547
Size: 5711
BamHI G'GATC_C
Cuts at: 2736 2736
Size: 5711
BanI G'GyrC_C
Cuts at: 981 2727 3141 3389 3424 981
Size: 1746 414 248 35 3268
Fragments arranged by size:
3268 1746 414 248 35
BanII G_rGCy'C
Cuts at: 1914 2725 3755 1914
Size: 811 1030 3870
Fragments arranged by size:
3870 1030 811
BbeI G_GCGC'C
Cuts at: 3145 3393 3145
Size: 248 5463
BbsI GAAGACnn'nnnn_
Cuts at: 1074 1074
Size: 5711
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 802 836 90
Size: 34 678 34 4965
Fragments arranged by size:
4965 678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3609 4370 5711 3609
Size: 761 1341 3609
Fragments arranged by size:
3609 1341 761
BclI T'GATC_A
Cuts at: 3231 3231
Size: 5711
BglI GCCn_nnn'nGGC
Cuts at: 112 990 112
Size: 878 4833
BglII A'GATC_T
Cuts at: 492 1796 3226 5263 492
Size: 1304 1430 2037 940
Fragments arranged by size:
2037 1430 1304 940
BlpI GC'TnA_GC
Cuts at: 2823 2823
Size: 5711
Bme1580I G_kGCm'C
Cuts at: 1584 3336 3429 4485 1584
Size: 1752 93 1056 2810
Fragments arranged by size:
2810 1752 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 888 2923 3004 3339 5483 888
Size: 2035 81 335 2144 1116
Fragments arranged by size:
2144 2035 1116 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4078 4078
Size: 5711
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2488 2488
Size: 5711
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 180 517 895 2887 3766 4258 4556 180
Size: 337 378 1992 879 492 298 1335
Fragments arranged by size:
1992 1335 879 492 378 337 298
BsaI GGTCTCn'nnnn_
Cuts at: 724 5415 724
Size: 4691 1020
BsaAI yAC'GTr
Cuts at: 1894 3064 1894
Size: 1170 4541
BsaBI GATnn'nnATC
Cuts at: 6 3225 5262 6
Size: 3219 2037 455
Fragments arranged by size:
3219 2037 455
BsaHI Gr'CG_yC
Cuts at: 3142 3390 4092 5588 3142
Size: 248 702 1496 3265
Fragments arranged by size:
3265 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2900 3421 4099 4373 4520 4931 2900
Size: 521 678 274 147 411 3680
Fragments arranged by size:
3680 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2020 2020
Size: 5711
BseYI C'CCAG_C
Cuts at: 3136 3894 4471 4967 3136
Size: 758 577 496 3880
Fragments arranged by size:
3880 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 116 3253 3299 4507 104
Size: 12 3137 46 1208 1308
Fragments arranged by size:
3137 1308 1208 46 12
BsiHKAI G_wGCw'C
Cuts at: 1504 1914 2725 3503 3693 4485 1504
Size: 410 811 778 190 792 2730
Fragments arranged by size:
2730 811 792 778 410 190
BsmI GAATG_Cn'
Cuts at: 144 144
Size: 5711
BsmBI CGTCTCn'nnnn_
Cuts at: 2254 2254
Size: 5711
Bsp1286I G_dGCh'C
Cuts at: 1504 1584 1914 2725 3336 3429 3503 3693 3755
Size: 80 330 811 611 93 74 190 62
Cuts at: 3755 4485 1504
Size: 730 2730
Fragments arranged by size:
2730 811 730 611 330 190 93 80
74 62
BspEI T'CCGG_A
Cuts at: 4931 4931
Size: 5711
BspHI T'CATG_A
Cuts at: 1632 1812 2232 4887 1632
Size: 180 420 2655 2456
Fragments arranged by size:
2655 2456 420 180
BspMI ACCTGCnnnn'nnnn_
Cuts at: 549 706 2761 2975 3277 3658 549
Size: 157 2055 214 302 381 2602
Fragments arranged by size:
2602 2055 381 302 214 157
BsrBI CCG'CTC
Cuts at: 50 4003 4057 5541 5706 50
Size: 3953 54 1484 165 55
Fragments arranged by size:
3953 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1604 2389 3623 1604
Size: 785 1234 3692
Fragments arranged by size:
3692 1234 785
BsrFI r'CCGG_y
Cuts at: 111 1175 3709 3890 4099 111
Size: 1064 2534 181 209 1723
Fragments arranged by size:
2534 1723 1064 209 181
BssSI C'ACGA_G
Cuts at: 1342 3982 4340 1342
Size: 2640 358 2713
Fragments arranged by size:
2713 2640 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1070 1786 3088 1070
Size: 716 1302 3693
Fragments arranged by size:
3693 1302 716
BstBI TT'CG_AA
Cuts at: 4072 4072
Size: 5711
BstXI CCAn_nnnn'nTGG
Cuts at: 978 978
Size: 5711
BstYI r'GATC_y
Cuts at: 1 155 492 1796 2433 2736 3203 3226 3561
Size: 154 337 1304 637 303 467 23 335
Cuts at: 3561 3807 4819 4905 4917 5263 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1304 1012 637 467 449 346 337 335
303 246 154 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1048 1048
Size: 5711
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3661 3842 4005 3661
Size: 181 163 5367
Fragments arranged by size:
5367 181 163
BtsI GCAGTG_nn'
Cuts at: 1561 2270 2954 1561
Size: 709 684 4318
Fragments arranged by size:
4318 709 684
DraI TTT'AAA
Cuts at: 1026 2710 1026
Size: 1684 4027
DrdI GACnn_nn'nnGTC
Cuts at: 3417 4275 3417
Size: 858 4853
EaeI y'GGCC_r
Cuts at: 113 2048 2693 3296 3470 3861 3888 5233 113
Size: 1935 645 603 174 391 27 1345 591
Fragments arranged by size:
1935 1345 645 603 591 391 174 27
EagI C'GGCC_G
Cuts at: 113 3296 113
Size: 3183 2528
EarI CTCTTCn'nnn_
Cuts at: 151 334 1130 1902 1931 2633 3734 3944 151
Size: 183 796 772 29 702 1101 210 1918
Fragments arranged by size:
1918 1101 796 772 702 210 183 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3919 4229 4375 5521 3919
Size: 310 146 1146 4109
Fragments arranged by size:
4109 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1912 2723 1912
Size: 811 4900
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 514 1511 2488 2705 3536 3968 4715 4983
Size: 156 997 977 217 831 432 747 268
Cuts at: 4983 358
Size: 1086
Fragments arranged by size:
1086 997 977 831 747 432 268 217
156
EcoO109I rG'GnC_Cy
Cuts at: 462 2850 462
Size: 2388 3323
EcoRI G'AATT_C
Cuts at: 2379 2715 2379
Size: 336 5375
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 174 463 820 1765 2047 3730 174
Size: 289 357 945 282 1683 2155
Fragments arranged by size:
2155 1683 945 357 289 282
FspI TGC'GCA
Cuts at: 545 3492 545
Size: 2947 2764
HaeII r_GCGC'y
Cuts at: 3145 3393 4415 5394 3145
Size: 248 1022 979 3462
Fragments arranged by size:
3462 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 951 983 2001 2033 2112 2144 3553 3583 3585
Size: 32 1018 32 79 32 1409 30 2
Cuts at: 3585 3615 951
Size: 30 3047
Fragments arranged by size:
3047 1409 1018 79 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2750 2750
Size: 5711
HindIII A'AGCT_T
Cuts at: 299 731 1807 2596 299
Size: 432 1076 789 3414
Fragments arranged by size:
3414 1076 789 432
KasI G'GCGC_C
Cuts at: 3141 3389 3141
Size: 248 5463
KpnI G_GTAC'C
Cuts at: 2731 2731
Size: 5711
MluI A'CGCG_T
Cuts at: 5144 5144
Size: 5711
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4382 4566 4382
Size: 184 5527
MscI TGG'CCA
Cuts at: 2050 2695 3472 2050
Size: 645 777 4289
Fragments arranged by size:
4289 777 645
MslI CAynn'nnrTG
Cuts at: 362 429 3700 3827 362
Size: 67 3271 127 2246
Fragments arranged by size:
3271 2246 127 67
MspA1I CmG'CkG
Cuts at: 1531 2410 2822 3136 3496 4509 4754 1531
Size: 879 412 314 360 1013 245 2488
Fragments arranged by size:
2488 1013 879 412 360 314 245
NaeI GCC'GGC
Cuts at: 113 3892 113
Size: 3779 1932
NarI GG'CG_CC
Cuts at: 3142 3390 3142
Size: 248 5463
NdeI CA'TA_TG
Cuts at: 1719 1719
Size: 5711
NgoMIV G'CCGG_C
Cuts at: 111 3890 111
Size: 3779 1932
NspI r_CATG'y
Cuts at: 1369 1464 1563 1869 2513 2764 3264 4171 1369
Size: 95 99 306 644 251 500 907 2909
Fragments arranged by size:
2909 907 644 500 306 251 99 95
PasI CC'CwG_GG
Cuts at: 971 971
Size: 5711
PciI A'CATG_T
Cuts at: 1865 2509 4167 1865
Size: 644 1658 3409
Fragments arranged by size:
3409 1658 644
PflMI CCAn_nnn'nTGG
Cuts at: 1416 1416
Size: 5711
PfoI T'CCnGG_A
Cuts at: 2628 2628
Size: 5711
PmeI GTTT'AAAC
Cuts at: 2710 2710
Size: 5711
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1325 1357 4850 4882 1325
Size: 32 3493 32 2154
Fragments arranged by size:
3493 2154 32 32
PpuMI rG'GwC_Cy
Cuts at: 462 462
Size: 5711
PsiI TTA'TAA
Cuts at: 1592 1592
Size: 5711
PstI C_TGCA'G
Cuts at: 840 2265 2679 2758 3443 840
Size: 1425 414 79 685 3108
Fragments arranged by size:
3108 1425 685 414 79
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5711
PvuII CAG'CTG
Cuts at: 2410 3136 3496 2410
Size: 726 360 4625
Fragments arranged by size:
4625 726 360
RsrII CG'GwC_CG
Cuts at: 3906 3906
Size: 5711
SacI G_AGCT'C
Cuts at: 1914 2725 1914
Size: 811 4900
SalI G'TCGA_C
Cuts at: 2748 2748
Size: 5711
SapI GCTCTTCn'nnn_
Cuts at: 151 1902 3734 3944 151
Size: 1751 1832 210 1918
Fragments arranged by size:
1918 1832 1751 210
SbfI CC_TGCA'GG
Cuts at: 2758 2758
Size: 5711
ScaI AGT'ACT
Cuts at: 1240 5228 1240
Size: 3988 1723
SfcI C'TryA_G
Cuts at: 33 475 723 782 836 1790 2261 2456 2675
Size: 442 248 59 54 954 471 195 219
Cuts at: 2675 2754 3439 4432 4623 33
Size: 79 685 993 191 1121
Fragments arranged by size:
1121 993 954 685 471 442 248 219
195 191 79 59 54
SfoI GGC'GCC
Cuts at: 3143 3391 3143
Size: 248 5463
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5711
SmaI CCC'GGG
Cuts at: 2733 2733
Size: 5711
SmlI C'TyrA_G
Cuts at: 159 456 496 910 1621 2866 3781 4273 4535
Size: 297 40 414 711 1245 915 492 262
Cuts at: 4535 159
Size: 1335
Fragments arranged by size:
1335 1245 915 711 492 414 297 262
40
SnaBI TAC'GTA
Cuts at: 1894 1894
Size: 5711
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5711
SphI G_CATG'C
Cuts at: 1369 2764 3264 1369
Size: 1395 500 3816
Fragments arranged by size:
3816 1395 500
SspI AAT'ATT
Cuts at: 1377 1377
Size: 5711
StuI AGG'CCT
Cuts at: 1414 1414
Size: 5711
StyI C'CwwG_G
Cuts at: 131 251 1415 2051 2547 2845 3199 131
Size: 120 1164 636 496 298 354 2643
Fragments arranged by size:
2643 1164 636 496 354 298 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3294 3427 3886 4095 3294
Size: 133 459 209 4910
Fragments arranged by size:
4910 459 209 133
TatI w'GTAC_w
Cuts at: 1238 1292 1315 1746 5226 1238
Size: 54 23 431 3480 1723
Fragments arranged by size:
3480 1723 431 54 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5228 5260 5228
Size: 32 5679
Tth111I GACn'n_nGTC
Cuts at: 3508 3508
Size: 5711
XbaI T'CTAG_A
Cuts at: 2742 2742
Size: 5711
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3206 3206
Size: 5711
XmaI C'CCGG_G
Cuts at: 2731 2731
Size: 5711
XmnI GAAnn'nnTTC
Cuts at: 311 1337 2033 2168 2366 311
Size: 1026 696 135 198 3656
Fragments arranged by size:
3656 1026 696 198 135
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI
BsgI BsiWI BsrGI BssHII BstEII BstZ17I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NcoI NheI NotI NruI NsiI PacI PmlI PshAI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI SwaI XhoI ZraI