Restriction map for
pF1KB0435
(Circular) (Six-base) MAPSORT of: pF1KB0435.seq Check: 1330 from: 1 to: 4457
pF1KB0435 4457 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1434 1434
Size: 4457
AccI GT'mk_AC
Cuts at: 959 1495 959
Size: 536 3921
Acc65I G'GTAC_C
Cuts at: 1473 1473
Size: 4457
AclI AA'CG_TT
Cuts at: 4297 4297
Size: 4457
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 548 760 1276 2282 2714 3461 3729 548
Size: 212 516 1006 432 747 268 1276
Fragments arranged by size:
1276 1006 747 516 432 268 212
AflIII A'CryG_T
Cuts at: 1112 1809 2913 3890 1112
Size: 697 1104 977 1679
Fragments arranged by size:
1679 1104 977 697
AgeI A'CCGG_T
Cuts at: 2845 2845
Size: 4457
AlwNI CAG_nnn'CTG
Cuts at: 945 1762 3329 945
Size: 817 1567 2073
Fragments arranged by size:
2073 1567 817
ApaLI G'TGCA_C
Cuts at: 3227 3227
Size: 4457
ApoI r'AATT_y
Cuts at: 868 1461 3810 4066 868
Size: 593 2349 256 1259
Fragments arranged by size:
2349 1259 593 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4457
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4457
AvaI C'yCGr_G
Cuts at: 179 594 780 1165 1477 179
Size: 415 186 385 312 3159
Fragments arranged by size:
3159 415 385 312 186
BamHI G'GATC_C
Cuts at: 1482 1482
Size: 4457
BanI G'GyrC_C
Cuts at: 295 528 589 1229 1473 1887 2135 2170 295
Size: 233 61 640 244 414 248 35 2582
Fragments arranged by size:
2582 640 414 248 244 233 61 35
BanII G_rGCy'C
Cuts at: 275 977 997 1471 2501 275
Size: 702 20 474 1030 2231
Fragments arranged by size:
2231 1030 702 474 20
BbeI G_GCGC'C
Cuts at: 1233 1891 2139 1233
Size: 658 248 3551
Fragments arranged by size:
3551 658 248
BbsI GAAGACnn'nnnn_
Cuts at: 748 1177 748
Size: 429 4028
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 901 935 1087 1121 901
Size: 34 152 34 4237
Fragments arranged by size:
4237 152 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2355 3116 4457 2355
Size: 761 1341 2355
Fragments arranged by size:
2355 1341 761
BclI T'GATC_A
Cuts at: 671 1977 671
Size: 1306 3151
BglI GCCn_nnn'nGGC
Cuts at: 461 461
Size: 4457
BglII A'GATC_T
Cuts at: 1972 4009 1972
Size: 2037 2420
BlpI GC'TnA_GC
Cuts at: 174 946 1186 1569 174
Size: 772 240 383 3062
Fragments arranged by size:
3062 772 383 240
Bme1580I G_kGCm'C
Cuts at: 250 2082 2175 3231 250
Size: 1832 93 1056 1476
Fragments arranged by size:
1832 1476 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1669 1750 2085 4229 1669
Size: 81 335 2144 1897
Fragments arranged by size:
2144 1897 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2824 2824
Size: 4457
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 496 875 1423 496
Size: 379 548 3530
Fragments arranged by size:
3530 548 379
Bpu10I CC'TnA_GC
Cuts at: 1334 1334
Size: 4457
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1633 2512 3004 3302 1633
Size: 879 492 298 2788
Fragments arranged by size:
2788 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4161 4161
Size: 4457
BsaAI yAC'GTr
Cuts at: 1196 1810 1196
Size: 614 3843
BsaBI GATnn'nnATC
Cuts at: 6 1971 4008 6
Size: 1965 2037 455
Fragments arranged by size:
2037 1965 455
BsaHI Gr'CG_yC
Cuts at: 136 372 1230 1888 2136 2838 4334 136
Size: 236 858 658 248 702 1496 259
Fragments arranged by size:
1496 858 702 658 259 248 236
BsaWI w'CCGG_w
Cuts at: 1646 2167 2845 3119 3266 3677 1646
Size: 521 678 274 147 411 2426
Fragments arranged by size:
2426 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 597 627 873 903 1372 1402 597
Size: 30 246 30 469 30 3652
Fragments arranged by size:
3652 469 246 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 283 832 985 283
Size: 549 153 3755
Fragments arranged by size:
3755 549 153
BseYI C'CCAG_C
Cuts at: 759 1433 1882 2640 3217 3713 759
Size: 674 449 758 577 496 1503
Fragments arranged by size:
1503 758 674 577 496 449
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 111 1375 1412 111
Size: 1264 37 3156
Fragments arranged by size:
3156 1264 37
BsiEI CG_ry'CG
Cuts at: 104 1999 2045 3253 104
Size: 1895 46 1208 1308
Fragments arranged by size:
1895 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 175 1471 2249 2439 3231 175
Size: 1296 778 190 792 1401
Fragments arranged by size:
1401 1296 792 778 190
BsmI GAATG_Cn'
Cuts at: 1187 1187
Size: 4457
Bsp1286I G_dGCh'C
Cuts at: 175 250 275 977 997 1471 2082 2175 2249
Size: 75 25 702 20 474 611 93 74
Cuts at: 2249 2439 2501 3231 175
Size: 190 62 730 1401
Fragments arranged by size:
1401 730 702 611 474 190 93 75
74 62 25 20
BspEI T'CCGG_A
Cuts at: 3677 3677
Size: 4457
BspHI T'CATG_A
Cuts at: 3633 3633
Size: 4457
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1434 1507 1721 2023 2404 1434
Size: 73 214 302 381 3487
Fragments arranged by size:
3487 381 302 214 73
BsrBI CCG'CTC
Cuts at: 50 184 1365 2749 2803 4287 4452 50
Size: 134 1181 1384 54 1484 165 55
Fragments arranged by size:
1484 1384 1181 165 134 55 54
BsrDI GCAATG_nn'
Cuts at: 674 686 2369 674
Size: 12 1683 2762
Fragments arranged by size:
2762 1683 12
BsrFI r'CCGG_y
Cuts at: 2455 2636 2845 2455
Size: 181 209 4067
Fragments arranged by size:
4067 209 181
BssSI C'ACGA_G
Cuts at: 2728 3086 2728
Size: 358 4099
BstAPI GCAn_nnn'nTGC
Cuts at: 509 1834 509
Size: 1325 3132
BstBI TT'CG_AA
Cuts at: 2818 2818
Size: 4457
BstYI r'GATC_y
Cuts at: 1 1091 1482 1949 1972 2307 2553 3565 3651
Size: 1090 391 467 23 335 246 1012 86
Cuts at: 3651 3663 4009 1
Size: 12 346 449
Fragments arranged by size:
1090 1012 467 449 391 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 1142 107
Size: 1035 3422
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 690 2407 2588 2751 690
Size: 1717 181 163 2396
Fragments arranged by size:
2396 1717 181 163
BtsI GCAGTG_nn'
Cuts at: 662 1700 662
Size: 1038 3419
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1098 1133 1098
Size: 35 4422
DraI TTT'AAA
Cuts at: 1456 1456
Size: 4457
DrdI GACnn_nn'nnGTC
Cuts at: 2163 3021 2163
Size: 858 3599
EaeI y'GGCC_r
Cuts at: 659 701 1031 2042 2216 2607 2634 3979 659
Size: 42 330 1011 174 391 27 1345 1137
Fragments arranged by size:
1345 1137 1011 391 330 174 42 27
EagI C'GGCC_G
Cuts at: 2042 2042
Size: 4457
EarI CTCTTCn'nnn_
Cuts at: 781 1252 2480 2690 781
Size: 471 1228 210 2548
Fragments arranged by size:
2548 1228 471 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 349 2665 2975 3121 4267 349
Size: 2316 310 146 1146 539
Fragments arranged by size:
2316 1146 539 310 146
EcoICRI GAG'CTC
Cuts at: 1469 1469
Size: 4457
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 496 548 760 875 1276 1423 2282 2714 3461
Size: 52 212 115 401 147 859 432 747
Cuts at: 3461 3729 496
Size: 268 1224
Fragments arranged by size:
1224 859 747 432 401 268 212 147
115 52
EcoO109I rG'GnC_Cy
Cuts at: 831 1596 831
Size: 765 3692
EcoRI G'AATT_C
Cuts at: 1461 1461
Size: 4457
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2476 2476
Size: 4457
FspI TGC'GCA
Cuts at: 2238 2238
Size: 4457
HaeII r_GCGC'y
Cuts at: 1233 1891 2139 3161 4140 1233
Size: 658 248 1022 979 1550
Fragments arranged by size:
1550 1022 979 658 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 873 905 910 942 2299 2329 2331 2361 873
Size: 32 5 32 1357 30 2 30 2969
Fragments arranged by size:
2969 1357 32 32 30 30 5 2
HincII GTy'rAC
Cuts at: 960 1496 960
Size: 536 3921
KasI G'GCGC_C
Cuts at: 1229 1887 2135 1229
Size: 658 248 3551
Fragments arranged by size:
3551 658 248
KpnI G_GTAC'C
Cuts at: 1477 1477
Size: 4457
MluI A'CGCG_T
Cuts at: 3890 3890
Size: 4457
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3128 3312 3128
Size: 184 4273
MscI TGG'CCA
Cuts at: 661 703 1033 2218 661
Size: 42 330 1185 2900
Fragments arranged by size:
2900 1185 330 42
MslI CAynn'nnrTG
Cuts at: 679 724 2446 2573 679
Size: 45 1722 127 2563
Fragments arranged by size:
2563 1722 127 45
MspA1I CmG'CkG
Cuts at: 942 1568 1882 2242 3255 3500 942
Size: 626 314 360 1013 245 1899
Fragments arranged by size:
1899 1013 626 360 314 245
NaeI GCC'GGC
Cuts at: 2638 2638
Size: 4457
NarI GG'CG_CC
Cuts at: 1230 1888 2136 1230
Size: 658 248 3551
Fragments arranged by size:
3551 658 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4457
NgoMIV G'CCGG_C
Cuts at: 2636 2636
Size: 4457
NspI r_CATG'y
Cuts at: 474 1293 1444 1510 2010 2917 474
Size: 819 151 66 500 907 2014
Fragments arranged by size:
2014 907 819 500 151 66
PciI A'CATG_T
Cuts at: 2913 2913
Size: 4457
PmeI GTTT'AAAC
Cuts at: 1456 1456
Size: 4457
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 907 939 3596 3628 907
Size: 32 2657 32 1736
Fragments arranged by size:
2657 1736 32 32
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 199 231 199
Size: 32 4425
PstI C_TGCA'G
Cuts at: 1504 2189 1504
Size: 685 3772
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4457
PvuII CAG'CTG
Cuts at: 942 1882 2242 942
Size: 940 360 3157
Fragments arranged by size:
3157 940 360
RsrII CG'GwC_CG
Cuts at: 2652 2652
Size: 4457
SacI G_AGCT'C
Cuts at: 1471 1471
Size: 4457
SalI G'TCGA_C
Cuts at: 958 1494 958
Size: 536 3921
SapI GCTCTTCn'nnn_
Cuts at: 781 2480 2690 781
Size: 1699 210 2548
Fragments arranged by size:
2548 1699 210
SbfI CC_TGCA'GG
Cuts at: 1504 1504
Size: 4457
ScaI AGT'ACT
Cuts at: 214 3974 214
Size: 3760 697
SfcI C'TryA_G
Cuts at: 33 1323 1500 2185 3178 3369 33
Size: 1290 177 685 993 191 1121
Fragments arranged by size:
1290 1121 993 685 191 177
SfoI GGC'GCC
Cuts at: 1231 1889 2137 1231
Size: 658 248 3551
Fragments arranged by size:
3551 658 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4457
SmaI CCC'GGG
Cuts at: 782 1479 782
Size: 697 3760
SmlI C'TyrA_G
Cuts at: 1165 1612 2527 3019 3281 1165
Size: 447 915 492 262 2341
Fragments arranged by size:
2341 915 492 447 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4457
SphI G_CATG'C
Cuts at: 1293 1510 2010 1293
Size: 217 500 3740
Fragments arranged by size:
3740 500 217
StyI C'CwwG_G
Cuts at: 107 542 648 1591 1945 107
Size: 435 106 943 354 2619
Fragments arranged by size:
2619 943 435 354 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 501 2040 2173 2632 2841 501
Size: 1539 133 459 209 2117
Fragments arranged by size:
2117 1539 459 209 133
TatI w'GTAC_w
Cuts at: 212 3972 212
Size: 3760 697
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 597 629 1372 1404 3974 4006 597
Size: 32 743 32 2570 32 1048
Fragments arranged by size:
2570 1048 743 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2254 2254
Size: 4457
XbaI T'CTAG_A
Cuts at: 1488 1488
Size: 4457
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 549 696 1952 114
Size: 435 147 1256 2619
Fragments arranged by size:
2619 1256 435 147
XhoI C'TCGA_G
Cuts at: 1165 1165
Size: 4457
XmaI C'CCGG_G
Cuts at: 780 1477 780
Size: 697 3760
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XmnI ZraI