Restriction map for
pF1KB0437
(Circular) (Six-base) MAPSORT of: pF1KB0437.seq Check: 8296 from: 1 to: 4556
pF1KB0437 4556 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AccI GT'mk_AC
Cuts at: 1594 1594
Size: 4556
Acc65I G'GTAC_C
Cuts at: 721 1572 721
Size: 851 3705
AclI AA'CG_TT
Cuts at: 4396 4396
Size: 4556
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 944 961 1010 1045 1246 1444 2381 2813 3560
Size: 17 49 35 201 198 937 432 747
Cuts at: 3560 3828 944
Size: 268 1672
Fragments arranged by size:
1672 937 747 432 268 201 198 49
35 17
AflIII A'CryG_T
Cuts at: 929 1908 3012 3989 929
Size: 979 1104 977 1496
Fragments arranged by size:
1496 1104 979 977
AgeI A'CCGG_T
Cuts at: 2944 2944
Size: 4556
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 421 453 1181 1213 421
Size: 32 728 32 3764
Fragments arranged by size:
3764 728 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1861 3428 1861
Size: 1567 2989
ApaLI G'TGCA_C
Cuts at: 3326 3326
Size: 4556
ApoI r'AATT_y
Cuts at: 563 937 1147 1560 3909 4165 563
Size: 374 210 413 2349 256 954
Fragments arranged by size:
2349 954 413 374 256 210
AseI AT'TA_AT
Cuts at: 20 20
Size: 4556
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4556
AvaI C'yCGr_G
Cuts at: 246 1576 246
Size: 1330 3226
BamHI G'GATC_C
Cuts at: 467 1581 467
Size: 1114 3442
BanI G'GyrC_C
Cuts at: 721 1483 1572 1986 2234 2269 721
Size: 762 89 414 248 35 3008
Fragments arranged by size:
3008 762 414 248 89 35
BanII G_rGCy'C
Cuts at: 528 744 985 1158 1570 2600 528
Size: 216 241 173 412 1030 2484
Fragments arranged by size:
2484 1030 412 241 216 173
BbeI G_GCGC'C
Cuts at: 1990 2238 1990
Size: 248 4308
BbsI GAAGACnn'nnnn_
Cuts at: 932 932
Size: 4556
BciVI GTATCCnnnnn_n'
Cuts at: 1128 2454 3215 4556 1128
Size: 1326 761 1341 1128
Fragments arranged by size:
1341 1326 1128 761
BclI T'GATC_A
Cuts at: 1313 2076 1313
Size: 763 3793
BfrBI ATG'CAT
Cuts at: 1121 1121
Size: 4556
BglII A'GATC_T
Cuts at: 945 1427 2071 4108 945
Size: 482 644 2037 1393
Fragments arranged by size:
2037 1393 644 482
BlpI GC'TnA_GC
Cuts at: 1668 1668
Size: 4556
Bme1580I G_kGCm'C
Cuts at: 1486 2181 2274 3330 1486
Size: 695 93 1056 2712
Fragments arranged by size:
2712 1056 695 93
BmgBI CAC'GTC
Cuts at: 930 1010 930
Size: 80 4476
BmrI ACTGGGnnnn_n'
Cuts at: 278 1489 1768 1849 2184 4328 278
Size: 1211 279 81 335 2144 506
Fragments arranged by size:
2144 1211 506 335 279 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2923 2923
Size: 4556
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 542 1214 1490 208
Size: 334 672 276 3274
Fragments arranged by size:
3274 672 334 276
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1732 2611 3103 3401 1732
Size: 879 492 298 2887
Fragments arranged by size:
2887 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1097 4260 1097
Size: 3163 1393
BsaAI yAC'GTr
Cuts at: 1909 1909
Size: 4556
BsaBI GATnn'nnATC
Cuts at: 6 2070 4107 6
Size: 2064 2037 455
Fragments arranged by size:
2064 2037 455
BsaHI Gr'CG_yC
Cuts at: 1987 2235 2937 4433 1987
Size: 248 702 1496 2110
Fragments arranged by size:
2110 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1745 2266 2944 3218 3365 3776 1745
Size: 521 678 274 147 411 2525
Fragments arranged by size:
2525 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 136 151 166 181 136
Size: 15 15 15 4511
Fragments arranged by size:
4511 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 186 211 1051 186
Size: 25 840 3691
Fragments arranged by size:
3691 840 25
BseYI C'CCAG_C
Cuts at: 1981 2739 3316 3812 1981
Size: 758 577 496 2725
Fragments arranged by size:
2725 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 342 567 812 342
Size: 225 245 4086
Fragments arranged by size:
4086 245 225
BsiEI CG_ry'CG
Cuts at: 104 2098 2144 3352 104
Size: 1994 46 1208 1308
Fragments arranged by size:
1994 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 744 1158 1185 1570 2348 2538 3330 744
Size: 414 27 385 778 190 792 1970
Fragments arranged by size:
1970 792 778 414 385 190 27
Bsp1286I G_dGCh'C
Cuts at: 528 744 985 1158 1185 1486 1570 2181 2274
Size: 216 241 173 27 301 84 611 93
Cuts at: 2274 2348 2538 2600 3330 528
Size: 74 190 62 730 1754
Fragments arranged by size:
1754 730 611 301 241 216 190 173
93 84 74 62 27
BspEI T'CCGG_A
Cuts at: 3776 3776
Size: 4556
BspHI T'CATG_A
Cuts at: 3732 3732
Size: 4556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1246 1606 1820 2122 2503 1246
Size: 360 214 302 381 3299
Fragments arranged by size:
3299 381 360 302 214
BsrBI CCG'CTC
Cuts at: 50 2848 2902 4386 4551 50
Size: 2798 54 1484 165 55
Fragments arranged by size:
2798 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 780 1047 2468 780
Size: 267 1421 2868
Fragments arranged by size:
2868 1421 267
BsrFI r'CCGG_y
Cuts at: 1456 2554 2735 2944 1456
Size: 1098 181 209 3068
Fragments arranged by size:
3068 1098 209 181
BssSI C'ACGA_G
Cuts at: 2827 3185 2827
Size: 358 4198
BstAPI GCAn_nnn'nTGC
Cuts at: 1933 1933
Size: 4556
BstBI TT'CG_AA
Cuts at: 2917 2917
Size: 4556
BstYI r'GATC_y
Cuts at: 1 216 467 945 1427 1581 2048 2071 2406
Size: 215 251 478 482 154 467 23 335
Cuts at: 2406 2652 3664 3750 3762 4108 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 482 478 467 449 346 335 251
246 215 154 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 866 2506 2687 2850 866
Size: 1640 181 163 2572
Fragments arranged by size:
2572 1640 181 163
BtsI GCAGTG_nn'
Cuts at: 800 1467 1799 800
Size: 667 332 3557
Fragments arranged by size:
3557 667 332
DraI TTT'AAA
Cuts at: 1555 1555
Size: 4556
DraIII CAC_nnn'GTG
Cuts at: 545 558 545
Size: 13 4543
DrdI GACnn_nn'nnGTC
Cuts at: 295 2262 3120 295
Size: 1967 858 1731
Fragments arranged by size:
1967 1731 858
EaeI y'GGCC_r
Cuts at: 582 1451 2141 2315 2706 2733 4078 582
Size: 869 690 174 391 27 1345 1060
Fragments arranged by size:
1345 1060 869 690 391 174 27
EagI C'GGCC_G
Cuts at: 2141 2141
Size: 4556
EarI CTCTTCn'nnn_
Cuts at: 204 448 980 2579 2789 204
Size: 244 532 1599 210 1971
Fragments arranged by size:
1971 1599 532 244 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2764 3074 3220 4366 2764
Size: 310 146 1146 2954
Fragments arranged by size:
2954 1146 310 146
EcoICRI GAG'CTC
Cuts at: 742 1156 1568 742
Size: 414 412 3730
Fragments arranged by size:
3730 414 412
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 542 944 961 1010 1045 1214 1246 1444
Size: 334 402 17 49 35 169 32 198
Cuts at: 1444 1490 2381 2813 3560 3828 208
Size: 46 891 432 747 268 936
Fragments arranged by size:
936 891 747 432 402 334 268 198
169 49 46 35 32 17
EcoO109I rG'GnC_Cy
Cuts at: 243 1189 1695 243
Size: 946 506 3104
Fragments arranged by size:
3104 946 506
EcoRI G'AATT_C
Cuts at: 1560 1560
Size: 4556
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 967 2575 967
Size: 1608 2948
FspI TGC'GCA
Cuts at: 2337 2337
Size: 4556
HaeII r_GCGC'y
Cuts at: 1990 2238 3260 4239 1990
Size: 248 1022 979 2307
Fragments arranged by size:
2307 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 136 168 478 510 2398 2428 2430 2460 136
Size: 32 310 32 1888 30 2 30 2232
Fragments arranged by size:
2232 1888 310 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1595 1595
Size: 4556
HindIII A'AGCT_T
Cuts at: 992 992
Size: 4556
KasI G'GCGC_C
Cuts at: 1986 2234 1986
Size: 248 4308
KpnI G_GTAC'C
Cuts at: 725 1576 725
Size: 851 3705
MluI A'CGCG_T
Cuts at: 3989 3989
Size: 4556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 404 3227 3411 404
Size: 2823 184 1549
Fragments arranged by size:
2823 1549 184
MscI TGG'CCA
Cuts at: 584 2317 584
Size: 1733 2823
MslI CAynn'nnrTG
Cuts at: 1334 2545 2672 1334
Size: 1211 127 3218
Fragments arranged by size:
3218 1211 127
MspA1I CmG'CkG
Cuts at: 207 478 1500 1667 1981 2341 3354 3599 207
Size: 271 1022 167 314 360 1013 245 1164
Fragments arranged by size:
1164 1022 1013 360 314 271 245 167
NaeI GCC'GGC
Cuts at: 1458 2737 1458
Size: 1279 3277
NarI GG'CG_CC
Cuts at: 1987 2235 1987
Size: 248 4308
NdeI CA'TA_TG
Cuts at: 1123 1308 1123
Size: 185 4371
NgoMIV G'CCGG_C
Cuts at: 1456 2735 1456
Size: 1279 3277
NsiI A_TGCA'T
Cuts at: 1123 1123
Size: 4556
NspI r_CATG'y
Cuts at: 411 621 1609 2109 3016 411
Size: 210 988 500 907 1951
Fragments arranged by size:
1951 988 907 500 210
PasI CC'CwG_GG
Cuts at: 172 172
Size: 4556
PciI A'CATG_T
Cuts at: 3012 3012
Size: 4556
PmeI GTTT'AAAC
Cuts at: 1555 1555
Size: 4556
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 562 594 3695 3727 562
Size: 32 3101 32 1391
Fragments arranged by size:
3101 1391 32 32
PpuMI rG'GwC_Cy
Cuts at: 243 1189 243
Size: 946 3610
PstI C_TGCA'G
Cuts at: 810 1603 2288 810
Size: 793 685 3078
Fragments arranged by size:
3078 793 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4556
PvuII CAG'CTG
Cuts at: 207 478 1981 2341 207
Size: 271 1503 360 2422
Fragments arranged by size:
2422 1503 360 271
RsrII CG'GwC_CG
Cuts at: 2751 2751
Size: 4556
SacI G_AGCT'C
Cuts at: 744 1158 1570 744
Size: 414 412 3730
Fragments arranged by size:
3730 414 412
SalI G'TCGA_C
Cuts at: 1593 1593
Size: 4556
SapI GCTCTTCn'nnn_
Cuts at: 980 2579 2789 980
Size: 1599 210 2747
Fragments arranged by size:
2747 1599 210
SbfI CC_TGCA'GG
Cuts at: 1603 1603
Size: 4556
ScaI AGT'ACT
Cuts at: 4073 4073
Size: 4556
SfcI C'TryA_G
Cuts at: 33 391 806 1599 2284 3277 3468 33
Size: 358 415 793 685 993 191 1121
Fragments arranged by size:
1121 993 793 685 415 358 191
SfoI GGC'GCC
Cuts at: 1988 2236 1988
Size: 248 4308
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4556
SmaI CCC'GGG
Cuts at: 1578 1578
Size: 4556
SmlI C'TyrA_G
Cuts at: 1711 2626 3118 3380 1711
Size: 915 492 262 2887
Fragments arranged by size:
2887 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4556
SphI G_CATG'C
Cuts at: 1609 2109 1609
Size: 500 4056
StuI AGG'CCT
Cuts at: 118 118
Size: 4556
StyI C'CwwG_G
Cuts at: 1690 2044 1690
Size: 354 4202
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2139 2272 2731 2940 2139
Size: 133 459 209 3755
Fragments arranged by size:
3755 459 209 133
TatI w'GTAC_w
Cuts at: 4071 4071
Size: 4556
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 151 183 4073 4105 151
Size: 32 3890 32 602
Fragments arranged by size:
3890 602 32 32
Tth111I GACn'n_nGTC
Cuts at: 2353 2353
Size: 4556
XbaI T'CTAG_A
Cuts at: 663 1587 663
Size: 924 3632
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 267 2051 267
Size: 1784 2772
XmaI C'CCGG_G
Cuts at: 1576 1576
Size: 4556
XmnI GAAnn'nnTTC
Cuts at: 947 974 947
Size: 27 4529
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NsiI NspI PasI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmtI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I BtgI ClaI CspCI EcoNI EcoRV FseI FspAI
HpaI MfeI NcoI NheI NotI NruI PacI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI