Restriction map for
pF1KB0442
(Circular) (Six-base) MAPSORT of: pF1KB0442.seq Check: 3689 from: 1 to: 5819
pF1KB0442 5819 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2448 2448
Size: 5819
AccI GT'mk_AC
Cuts at: 2099 2857 2099
Size: 758 5061
Acc65I G'GTAC_C
Cuts at: 2416 2835 2416
Size: 419 5400
AclI AA'CG_TT
Cuts at: 5659 5659
Size: 5819
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 591 926 1284 1708 2198 3644 4076 4823 5091
Size: 335 358 424 490 1446 432 747 268
Cuts at: 5091 591
Size: 1319
Fragments arranged by size:
1446 1319 747 490 432 424 358 335
268
AfeI AGC'GCT
Cuts at: 497 1846 497
Size: 1349 4470
AflIII A'CryG_T
Cuts at: 1750 1752 1776 3171 4275 5252 1750
Size: 2 24 1395 1104 977 2317
Fragments arranged by size:
2317 1395 1104 977 24 2
AgeI A'CCGG_T
Cuts at: 4207 4207
Size: 5819
AleI CACnn'nnGTG
Cuts at: 1621 1621
Size: 5819
AlwNI CAG_nnn'CTG
Cuts at: 1860 1892 3124 4691 1860
Size: 32 1232 1567 2988
Fragments arranged by size:
2988 1567 1232 32
ApaI G_GGCC'C
Cuts at: 872 1068 1401 2369 872
Size: 196 333 968 4322
Fragments arranged by size:
4322 968 333 196
ApaLI G'TGCA_C
Cuts at: 1558 4589 1558
Size: 3031 2788
ApoI r'AATT_y
Cuts at: 142 1698 2823 5172 5428 142
Size: 1556 1125 2349 256 533
Fragments arranged by size:
2349 1556 1125 533 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5819
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5819
AvaI C'yCGr_G
Cuts at: 439 761 927 1025 1032 2059 2839 439
Size: 322 166 98 7 1027 780 3419
Fragments arranged by size:
3419 1027 780 322 166 98 7
AvrII C'CTAG_G
Cuts at: 1470 1470
Size: 5819
BamHI G'GATC_C
Cuts at: 2844 2844
Size: 5819
BanI G'GyrC_C
Cuts at: 355 1615 1824 2416 2835 3249 3497 3532 355
Size: 1260 209 592 419 414 248 35 2642
Fragments arranged by size:
2642 1260 592 419 414 248 209 35
BanII G_rGCy'C
Cuts at: 260 745 872 1039 1068 1389 1401 2183 2369
Size: 485 127 167 29 321 12 782 186
Cuts at: 2369 2497 2667 2833 3863 260
Size: 128 170 166 1030 2216
Fragments arranged by size:
2216 1030 782 485 321 186 170 167
166 128 127 29 12
BbeI G_GCGC'C
Cuts at: 3253 3501 3253
Size: 248 5571
BbsI GAAGACnn'nnnn_
Cuts at: 1099 2297 1099
Size: 1198 4621
BbvCI CC'TCA_GC
Cuts at: 1363 2618 1363
Size: 1255 4564
BciVI GTATCCnnnnn_n'
Cuts at: 2617 3717 4478 5819 2617
Size: 1100 761 1341 2617
Fragments arranged by size:
2617 1341 1100 761
BclI T'GATC_A
Cuts at: 3339 3339
Size: 5819
BfrBI ATG'CAT
Cuts at: 321 321
Size: 5819
BglI GCCn_nnn'nGGC
Cuts at: 112 824 112
Size: 712 5107
BglII A'GATC_T
Cuts at: 3334 5371 3334
Size: 2037 3782
BlpI GC'TnA_GC
Cuts at: 1848 2931 1848
Size: 1083 4736
Bme1580I G_kGCm'C
Cuts at: 872 1068 1250 1401 1562 1784 1829 2369 2433
Size: 196 182 151 161 222 45 540 64
Cuts at: 2433 3444 3537 4593 872
Size: 1011 93 1056 2098
Fragments arranged by size:
2098 1056 1011 540 222 196 182 161
151 93 64 45
BmrI ACTGGGnnnn_n'
Cuts at: 1880 3031 3112 3447 5591 1880
Size: 1151 81 335 2144 2108
Fragments arranged by size:
2144 2108 1151 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 741 1103 1167 4186 741
Size: 362 64 3019 2374
Fragments arranged by size:
3019 2374 362 64
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 819 1184 1378 2761 2816 520
Size: 299 365 194 1383 55 3523
Fragments arranged by size:
3523 1383 365 299 194 55
Bpu10I CC'TnA_GC
Cuts at: 1363 2618 1363
Size: 1255 4564
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2995 3874 4366 4664 2995
Size: 879 492 298 4150
Fragments arranged by size:
4150 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2762 5523 2762
Size: 2761 3058
BsaAI yAC'GTr
Cuts at: 1753 1779 3172 1753
Size: 26 1393 4400
Fragments arranged by size:
4400 1393 26
BsaBI GATnn'nnATC
Cuts at: 6 1507 2603 3333 5370 6
Size: 1501 1096 730 2037 455
Fragments arranged by size:
2037 1501 1096 730 455
BsaHI Gr'CG_yC
Cuts at: 3250 3498 4200 5696 3250
Size: 248 702 1496 3373
Fragments arranged by size:
3373 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2187 2667 3008 3529 4207 4481 4628 5039 2187
Size: 480 341 521 678 274 147 411 2967
Fragments arranged by size:
2967 678 521 480 411 341 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1021 1051 1021
Size: 30 5789
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 148 844 1156 1187 1198 1919 2014 2017 2191
Size: 696 312 31 11 721 95 3 174
Cuts at: 2191 2487 2540 148
Size: 296 53 3427
Fragments arranged by size:
3427 721 696 312 296 174 95 53
31 11 3
BseYI C'CCAG_C
Cuts at: 183 390 412 421 448 876 951 1339 1841
Size: 207 22 9 27 428 75 388 502
Cuts at: 1841 3244 4002 4579 5075 183
Size: 1403 758 577 496 927
Fragments arranged by size:
1403 927 758 577 502 496 428 388
207 75 27 22 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 2771 260
Size: 2511 3308
BsiEI CG_ry'CG
Cuts at: 104 2545 3361 3407 4615 104
Size: 2441 816 46 1208 1308
Fragments arranged by size:
2441 1308 1208 816 46
BsiHKAI G_wGCw'C
Cuts at: 864 1562 1840 2833 3611 3801 4593 864
Size: 698 278 993 778 190 792 2090
Fragments arranged by size:
2090 993 792 778 698 278 190
BsmI GAATG_Cn'
Cuts at: 321 321
Size: 5819
BsmBI CGTCTCn'nnnn_
Cuts at: 1720 1809 1720
Size: 89 5730
Bsp1286I G_dGCh'C
Cuts at: 260 745 864 872 1039 1068 1250 1389 1401
Size: 485 119 8 167 29 182 139 12
Cuts at: 1401 1562 1784 1829 1840 2183 2369 2433 2497
Size: 161 222 45 11 343 186 64 64
Cuts at: 2497 2667 2833 3444 3537 3611 3801 3863 4593
Size: 170 166 611 93 74 190 62 730
Cuts at: 4593 260
Size: 1486
Fragments arranged by size:
1486 730 611 485 343 222 190 186
182 170 167 166 161 139 119 93
74 64 64 62 45 29 12 11
8
BspEI T'CCGG_A
Cuts at: 2667 5039 2667
Size: 2372 3447
BspHI T'CATG_A
Cuts at: 4995 4995
Size: 5819
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1541 2448 2622 2869 3083 3385 3766 1541
Size: 907 174 247 214 302 381 3594
Fragments arranged by size:
3594 907 381 302 247 214 174
BsrBI CCG'CTC
Cuts at: 50 4111 4165 5649 5814 50
Size: 4061 54 1484 165 55
Fragments arranged by size:
4061 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1012 1164 1999 3731 1012
Size: 152 835 1732 3100
Fragments arranged by size:
3100 1732 835 152
BsrFI r'CCGG_y
Cuts at: 3817 3998 4207 3817
Size: 181 209 5429
Fragments arranged by size:
5429 209 181
BssSI C'ACGA_G
Cuts at: 639 4090 4448 639
Size: 3451 358 2010
Fragments arranged by size:
3451 2010 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3196 3196
Size: 5819
BstBI TT'CG_AA
Cuts at: 4180 4180
Size: 5819
BstEII G'GTnAC_C
Cuts at: 1450 1450
Size: 5819
BstXI CCAn_nnnn'nTGG
Cuts at: 1262 1328 1626 1262
Size: 66 298 5455
Fragments arranged by size:
5455 298 66
BstYI r'GATC_y
Cuts at: 1 792 2844 3311 3334 3669 3915 4927 5013
Size: 791 2052 467 23 335 246 1012 86
Cuts at: 5013 5025 5371 1
Size: 12 346 449
Fragments arranged by size:
2052 1012 791 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 778 886 966 1422 1586 107
Size: 671 108 80 456 164 4340
Fragments arranged by size:
4340 671 456 164 108 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3769 3950 4113 3769
Size: 181 163 5475
Fragments arranged by size:
5475 181 163
BtsI GCAGTG_nn'
Cuts at: 1127 1250 1514 3062 1127
Size: 123 264 1548 3884
Fragments arranged by size:
3884 1548 264 123
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 995 1030 995
Size: 35 5784
DraI TTT'AAA
Cuts at: 2818 2818
Size: 5819
DrdI GACnn_nn'nnGTC
Cuts at: 3525 4383 3525
Size: 858 4961
EaeI y'GGCC_r
Cuts at: 116 429 775 1583 1589 1661 2086 2542 2763
Size: 313 346 808 6 72 425 456 221
Cuts at: 2763 3404 3578 3969 3996 5341 116
Size: 641 174 391 27 1345 594
Fragments arranged by size:
1345 808 641 594 456 425 391 346
313 221 174 72 27 6
EagI C'GGCC_G
Cuts at: 2542 3404 2542
Size: 862 4957
EarI CTCTTCn'nnn_
Cuts at: 977 2558 2795 3842 4052 977
Size: 1581 237 1047 210 2744
Fragments arranged by size:
2744 1581 1047 237 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1116 2136 2516 4027 4337 4483 5629 1116
Size: 1020 380 1511 310 146 1146 1306
Fragments arranged by size:
1511 1306 1146 1020 380 310 146
EcoICRI GAG'CTC
Cuts at: 2831 2831
Size: 5819
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 591 819 926 1184 1284 1378 1708 2198
Size: 71 228 107 258 100 94 330 490
Cuts at: 2198 2761 2816 3644 4076 4823 5091 520
Size: 563 55 828 432 747 268 1248
Fragments arranged by size:
1248 828 747 563 490 432 330 268
258 228 107 100 94 71 55
EcoNI CCTnn'n_nnAGG
Cuts at: 1202 1202
Size: 5819
EcoO109I rG'GnC_Cy
Cuts at: 174 599 1029 1064 1065 1304 1975 2264 2365
Size: 425 430 35 1 239 671 289 101
Cuts at: 2365 2366 2578 2958 174
Size: 1 212 380 3035
Fragments arranged by size:
3035 671 430 425 380 289 239 212
101 35 1 1
EcoRI G'AATT_C
Cuts at: 1698 2823 1698
Size: 1125 4694
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1188 3838 1188
Size: 2650 3169
FspI TGC'GCA
Cuts at: 3600 3600
Size: 5819
HaeII r_GCGC'y
Cuts at: 275 499 918 1848 3253 3501 4523 5502 275
Size: 224 419 930 1405 248 1022 979 592
Fragments arranged by size:
1405 1022 979 930 592 419 248 224
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 688 720 1021 1053 2702 2734 3661 3691 3693
Size: 32 301 32 1649 32 927 30 2
Cuts at: 3693 3723 688
Size: 30 2784
Fragments arranged by size:
2784 1649 927 301 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2858 2858
Size: 5819
HindIII A'AGCT_T
Cuts at: 1690 1690
Size: 5819
KasI G'GCGC_C
Cuts at: 3249 3497 3249
Size: 248 5571
KpnI G_GTAC'C
Cuts at: 2420 2839 2420
Size: 419 5400
MluI A'CGCG_T
Cuts at: 5252 5252
Size: 5819
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1607 1958 4490 4674 1607
Size: 351 2532 184 2752
Fragments arranged by size:
2752 2532 351 184
MscI TGG'CCA
Cuts at: 1585 3580 1585
Size: 1995 3824
MslI CAynn'nnrTG
Cuts at: 1621 2242 3808 3935 1621
Size: 621 1566 127 3505
Fragments arranged by size:
3505 1566 621 127
MspA1I CmG'CkG
Cuts at: 122 183 750 888 1075 1356 2702 2930 3244
Size: 61 567 138 187 281 1346 228 314
Cuts at: 3244 3604 4617 4862 122
Size: 360 1013 245 1079
Fragments arranged by size:
1346 1079 1013 567 360 314 281 245
228 187 138 61
NaeI GCC'GGC
Cuts at: 4000 4000
Size: 5819
NarI GG'CG_CC
Cuts at: 3250 3498 3250
Size: 248 5571
NcoI C'CATG_G
Cuts at: 107 778 107
Size: 671 5148
NgoMIV G'CCGG_C
Cuts at: 3998 3998
Size: 5819
NsiI A_TGCA'T
Cuts at: 323 323
Size: 5819
NspI r_CATG'y
Cuts at: 321 2343 2872 3372 4279 321
Size: 2022 529 500 907 1861
Fragments arranged by size:
2022 1861 907 529 500
PasI CC'CwG_GG
Cuts at: 1785 2508 2653 1785
Size: 723 145 4951
Fragments arranged by size:
4951 723 145
PciI A'CATG_T
Cuts at: 4275 4275
Size: 5819
PflMI CCAn_nnn'nTGG
Cuts at: 619 2647 619
Size: 2028 3791
PfoI T'CCnGG_A
Cuts at: 1200 1210 2793 1200
Size: 10 1583 4226
Fragments arranged by size:
4226 1583 10
PmeI GTTT'AAAC
Cuts at: 2818 2818
Size: 5819
PmlI CAC'GTG
Cuts at: 1753 1779 1753
Size: 26 5793
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4958 4990 4958
Size: 32 5787
PpuMI rG'GwC_Cy
Cuts at: 1029 1304 1975 1029
Size: 275 671 4873
Fragments arranged by size:
4873 671 275
PspOMI G'GGCC_C
Cuts at: 868 1064 1397 2365 868
Size: 196 333 968 4322
Fragments arranged by size:
4322 968 333 196
PstI C_TGCA'G
Cuts at: 1521 2027 2445 2866 3551 1521
Size: 506 418 421 685 3789
Fragments arranged by size:
3789 685 506 421 418
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5819
PvuII CAG'CTG
Cuts at: 122 1075 1356 3244 3604 122
Size: 953 281 1888 360 2337
Fragments arranged by size:
2337 1888 953 360 281
RsrII CG'GwC_CG
Cuts at: 4014 4014
Size: 5819
SacI G_AGCT'C
Cuts at: 2833 2833
Size: 5819
SacII CC_GC'GG
Cuts at: 889 889
Size: 5819
SalI G'TCGA_C
Cuts at: 2856 2856
Size: 5819
SanDI GG'GwC_CC
Cuts at: 1029 1029
Size: 5819
SapI GCTCTTCn'nnn_
Cuts at: 2795 3842 4052 2795
Size: 1047 210 4562
Fragments arranged by size:
4562 1047 210
SbfI CC_TGCA'GG
Cuts at: 2866 2866
Size: 5819
ScaI AGT'ACT
Cuts at: 5336 5336
Size: 5819
SfcI C'TryA_G
Cuts at: 33 570 1476 1498 1517 1878 2023 2379 2441
Size: 537 906 22 19 361 145 356 62
Cuts at: 2441 2694 2862 3547 4540 4731 33
Size: 253 168 685 993 191 1121
Fragments arranged by size:
1121 993 906 685 537 361 356 253
191 168 145 62 22 19
SfoI GGC'GCC
Cuts at: 3251 3499 3251
Size: 248 5571
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5819
SmaI CCC'GGG
Cuts at: 763 1027 1034 2841 763
Size: 264 7 1807 3741
Fragments arranged by size:
3741 1807 264 7
SmlI C'TyrA_G
Cuts at: 439 2974 3889 4381 4643 439
Size: 2535 915 492 262 1615
Fragments arranged by size:
2535 1615 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5819
SphI G_CATG'C
Cuts at: 321 2343 2872 3372 321
Size: 2022 529 500 2768
Fragments arranged by size:
2768 2022 529 500
StuI AGG'CCT
Cuts at: 1876 1876
Size: 5819
StyI C'CwwG_G
Cuts at: 107 248 778 1172 1268 1470 1619 1952 2953
Size: 141 530 394 96 202 149 333 1001
Cuts at: 2953 3307 107
Size: 354 2619
Fragments arranged by size:
2619 1001 530 394 354 333 202 149
141 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1124 2274 3402 3535 3994 4203 1124
Size: 1150 1128 133 459 209 2740
Fragments arranged by size:
2740 1150 1128 459 209 133
TatI w'GTAC_w
Cuts at: 1809 2063 5334 1809
Size: 254 3271 2294
Fragments arranged by size:
3271 2294 254
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5336 5368 5336
Size: 32 5787
Tth111I GACn'n_nGTC
Cuts at: 2716 3616 2716
Size: 900 4919
XbaI T'CTAG_A
Cuts at: 2850 2850
Size: 5819
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 842 3314 842
Size: 2472 3347
XhoI C'TCGA_G
Cuts at: 439 439
Size: 5819
XmaI C'CCGG_G
Cuts at: 761 1025 1032 2839 761
Size: 264 7 1807 3741
Fragments arranged by size:
3741 1807 264 7
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI BaeI BcgI
BmgBI BmtI BsiWI BsrGI BssHII BstZ17I Bsu36I ClaI
DraIII EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI PacI PshAI PsiI PspXI PsrI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XmnI ZraI