Restriction map for
pF1KB0447
(Circular) (Six-base) MAPSORT of: pF1KB0447.seq Check: 848 from: 1 to: 4616
pF1KB0447 4616 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AccI GT'mk_AC
Cuts at: 1654 1654
Size: 4616
Acc65I G'GTAC_C
Cuts at: 1632 1632
Size: 4616
AclI AA'CG_TT
Cuts at: 4456 4456
Size: 4616
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 786 873 2441 2873 3620 3888 568
Size: 218 87 1568 432 747 268 1296
Fragments arranged by size:
1568 1296 747 432 268 218 87
AfeI AGC'GCT
Cuts at: 156 1577 156
Size: 1421 3195
AflIII A'CryG_T
Cuts at: 642 1968 3072 4049 642
Size: 1326 1104 977 1209
Fragments arranged by size:
1326 1209 1104 977
AgeI A'CCGG_T
Cuts at: 3004 3004
Size: 4616
AleI CACnn'nnGTG
Cuts at: 1426 1474 1426
Size: 48 4568
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 487 663 967 487
Size: 176 304 4136
Fragments arranged by size:
4136 304 176
AlwNI CAG_nnn'CTG
Cuts at: 324 1921 3488 324
Size: 1597 1567 1452
Fragments arranged by size:
1597 1567 1452
ApaLI G'TGCA_C
Cuts at: 3386 3386
Size: 4616
ApoI r'AATT_y
Cuts at: 398 1081 1549 1620 3969 4225 398
Size: 683 468 71 2349 256 789
Fragments arranged by size:
2349 789 683 468 256 71
AseI AT'TA_AT
Cuts at: 20 20
Size: 4616
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4616
AvaI C'yCGr_G
Cuts at: 854 1636 854
Size: 782 3834
BamHI G'GATC_C
Cuts at: 1641 1641
Size: 4616
BanI G'GyrC_C
Cuts at: 168 222 1632 2046 2294 2329 168
Size: 54 1410 414 248 35 2455
Fragments arranged by size:
2455 1410 414 248 54 35
BanII G_rGCy'C
Cuts at: 356 557 706 833 863 974 1244 1630 2660
Size: 201 149 127 30 111 270 386 1030
Cuts at: 2660 356
Size: 2312
Fragments arranged by size:
2312 1030 386 270 201 149 127 111
30
BbeI G_GCGC'C
Cuts at: 172 2050 2298 172
Size: 1878 248 2490
Fragments arranged by size:
2490 1878 248
BbsI GAAGACnn'nnnn_
Cuts at: 277 1406 277
Size: 1129 3487
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4582
BciVI GTATCCnnnnn_n'
Cuts at: 861 2514 3275 4616 861
Size: 1653 761 1341 861
Fragments arranged by size:
1653 1341 861 761
BclI T'GATC_A
Cuts at: 2136 2136
Size: 4616
BglI GCCn_nnn'nGGC
Cuts at: 671 671
Size: 4616
BglII A'GATC_T
Cuts at: 1184 2131 4168 1184
Size: 947 2037 1632
Fragments arranged by size:
2037 1632 947
BlpI GC'TnA_GC
Cuts at: 475 1025 1226 1728 475
Size: 550 201 502 3363
Fragments arranged by size:
3363 550 502 201
Bme1580I G_kGCm'C
Cuts at: 183 218 2241 2334 3390 183
Size: 35 2023 93 1056 1409
Fragments arranged by size:
2023 1409 1056 93 35
BmrI ACTGGGnnnn_n'
Cuts at: 170 1374 1828 1909 2244 4388 170
Size: 1204 454 81 335 2144 398
Fragments arranged by size:
2144 1204 454 398 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2983 2983
Size: 4616
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 234 340 772 1240 1360 1518 234
Size: 106 432 468 120 158 3332
Fragments arranged by size:
3332 468 432 158 120 106
Bpu10I CC'TnA_GC
Cuts at: 660 660
Size: 4616
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 1027 1792 2671 3163 3461 703
Size: 324 765 879 492 298 1858
Fragments arranged by size:
1858 879 765 492 324 298
BsaI GGTCTCn'nnnn_
Cuts at: 345 517 568 1552 4320 345
Size: 172 51 984 2768 641
Fragments arranged by size:
2768 984 641 172 51
BsaAI yAC'GTr
Cuts at: 1969 1969
Size: 4616
BsaBI GATnn'nnATC
Cuts at: 6 2130 4167 6
Size: 2124 2037 455
Fragments arranged by size:
2124 2037 455
BsaHI Gr'CG_yC
Cuts at: 169 193 2047 2295 2997 4493 169
Size: 24 1854 248 702 1496 292
Fragments arranged by size:
1854 1496 702 292 248 24
BsaWI w'CCGG_w
Cuts at: 902 1106 1462 1805 2326 3004 3278 3425 3836
Size: 204 356 343 521 678 274 147 411
Cuts at: 3836 902
Size: 1682
Fragments arranged by size:
1682 678 521 411 356 343 274 204
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 156 186 1174 1201 1204 1231 1315 1345 156
Size: 30 988 27 3 27 84 30 3427
Fragments arranged by size:
3427 988 84 30 30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 218 700 1320 1621 218
Size: 482 620 301 3213
Fragments arranged by size:
3213 620 482 301
BseYI C'CCAG_C
Cuts at: 2041 2799 3376 3872 2041
Size: 758 577 496 2785
Fragments arranged by size:
2785 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 762 2158 2204 3412 104
Size: 658 1396 46 1208 1308
Fragments arranged by size:
1396 1308 1208 658 46
BsiHKAI G_wGCw'C
Cuts at: 356 557 706 833 1244 1630 2408 2598 3390
Size: 201 149 127 411 386 778 190 792
Cuts at: 3390 356
Size: 1582
Fragments arranged by size:
1582 792 778 411 386 201 190 149
127
BsiWI C'GTAC_G
Cuts at: 965 965
Size: 4616
Bsp1286I G_dGCh'C
Cuts at: 183 218 356 557 706 833 863 974 1244
Size: 35 138 201 149 127 30 111 270
Cuts at: 1244 1630 2241 2334 2408 2598 2660 3390 183
Size: 386 611 93 74 190 62 730 1409
Fragments arranged by size:
1409 730 611 386 270 201 190 149
138 127 111 93 74 62 35 30
BspEI T'CCGG_A
Cuts at: 3836 3836
Size: 4616
BspHI T'CATG_A
Cuts at: 3792 3792
Size: 4616
BspMI ACCTGCnnnn'nnnn_
Cuts at: 102 309 631 890 1666 1880 2182 2563 102
Size: 207 322 259 776 214 302 381 2155
Fragments arranged by size:
2155 776 381 322 302 259 214 207
BsrBI CCG'CTC
Cuts at: 50 2908 2962 4446 4611 50
Size: 2858 54 1484 165 55
Fragments arranged by size:
2858 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 576 2528 576
Size: 1952 2664
BsrFI r'CCGG_y
Cuts at: 761 2614 2795 3004 761
Size: 1853 181 209 2373
Fragments arranged by size:
2373 1853 209 181
BssHII G'CGCG_C
Cuts at: 208 208
Size: 4616
BssSI C'ACGA_G
Cuts at: 1018 2887 3245 1018
Size: 1869 358 2389
Fragments arranged by size:
2389 1869 358
BstAPI GCAn_nnn'nTGC
Cuts at: 222 1993 222
Size: 1771 2845
BstBI TT'CG_AA
Cuts at: 2977 2977
Size: 4616
BstEII G'GTnAC_C
Cuts at: 730 1419 730
Size: 689 3927
BstYI r'GATC_y
Cuts at: 1 1124 1184 1641 2108 2131 2466 2712 3724
Size: 1123 60 457 467 23 335 246 1012
Cuts at: 3724 3810 3822 4168 1
Size: 86 12 346 449
Fragments arranged by size:
1123 1012 467 457 449 346 335 246
86 60 23 12
BtgI C'CryG_G
Cuts at: 1034 1139 1424 1562 1034
Size: 105 285 138 4088
Fragments arranged by size:
4088 285 138 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2566 2747 2910 2566
Size: 181 163 4272
Fragments arranged by size:
4272 181 163
BtsI GCAGTG_nn'
Cuts at: 469 1859 469
Size: 1390 3226
DraI TTT'AAA
Cuts at: 1615 1615
Size: 4616
DraIII CAC_nnn'GTG
Cuts at: 222 222
Size: 4616
DrdI GACnn_nn'nnGTC
Cuts at: 337 2322 3180 337
Size: 1985 858 1773
Fragments arranged by size:
1985 1773 858
EaeI y'GGCC_r
Cuts at: 672 2201 2375 2766 2793 4138 672
Size: 1529 174 391 27 1345 1150
Fragments arranged by size:
1529 1345 1150 391 174 27
EagI C'GGCC_G
Cuts at: 2201 2201
Size: 4616
EarI CTCTTCn'nnn_
Cuts at: 452 590 838 2639 2849 452
Size: 138 248 1801 210 2219
Fragments arranged by size:
2219 1801 248 210 138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1117 1582 2824 3134 3280 4426 1117
Size: 465 1242 310 146 1146 1307
Fragments arranged by size:
1307 1242 1146 465 310 146
EcoICRI GAG'CTC
Cuts at: 354 555 704 831 1242 1628 354
Size: 201 149 127 411 386 3342
Fragments arranged by size:
3342 411 386 201 149 127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 234 340 568 772 786 873 1240 1360 1518
Size: 106 228 204 14 87 367 120 158
Cuts at: 1518 2441 2873 3620 3888 234
Size: 923 432 747 268 962
Fragments arranged by size:
962 923 747 432 367 268 228 204
158 120 106 87 14
EcoO109I rG'GnC_Cy
Cuts at: 321 1755 321
Size: 1434 3182
EcoRI G'AATT_C
Cuts at: 1081 1620 1081
Size: 539 4077
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2635 2635
Size: 4616
FspI TGC'GCA
Cuts at: 2397 2397
Size: 4616
HaeII r_GCGC'y
Cuts at: 158 172 1579 2050 2298 3320 4299 158
Size: 14 1407 471 248 1022 979 475
Fragments arranged by size:
1407 1022 979 475 471 248 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 258 290 420 452 1174 1206 2458 2488 2490
Size: 32 130 32 722 32 1252 30 2
Cuts at: 2490 2520 258
Size: 30 2354
Fragments arranged by size:
2354 1252 722 130 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 414 1274 1655 414
Size: 860 381 3375
Fragments arranged by size:
3375 860 381
KasI G'GCGC_C
Cuts at: 168 2046 2294 168
Size: 1878 248 2490
Fragments arranged by size:
2490 1878 248
KpnI G_GTAC'C
Cuts at: 1636 1636
Size: 4616
MluI A'CGCG_T
Cuts at: 642 4049 642
Size: 3407 1209
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3287 3471 3287
Size: 184 4432
MscI TGG'CCA
Cuts at: 2377 2377
Size: 4616
MslI CAynn'nnrTG
Cuts at: 1426 1474 1510 2605 2732 1426
Size: 48 36 1095 127 3310
Fragments arranged by size:
3310 1095 127 48 36
MspA1I CmG'CkG
Cuts at: 228 1347 1727 2041 2401 3414 3659 228
Size: 1119 380 314 360 1013 245 1185
Fragments arranged by size:
1185 1119 1013 380 360 314 245
NaeI GCC'GGC
Cuts at: 2797 2797
Size: 4616
NarI GG'CG_CC
Cuts at: 169 2047 2295 169
Size: 1878 248 2490
Fragments arranged by size:
2490 1878 248
NcoI C'CATG_G
Cuts at: 1034 1034
Size: 4616
NgoMIV G'CCGG_C
Cuts at: 2795 2795
Size: 4616
NspI r_CATG'y
Cuts at: 780 1669 2169 3076 780
Size: 889 500 907 2320
Fragments arranged by size:
2320 907 889 500
PciI A'CATG_T
Cuts at: 3072 3072
Size: 4616
PfoI T'CCnGG_A
Cuts at: 356 836 1534 356
Size: 480 698 3438
Fragments arranged by size:
3438 698 480
PmeI GTTT'AAAC
Cuts at: 1615 1615
Size: 4616
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 688 720 3755 3787 688
Size: 32 3035 32 1517
Fragments arranged by size:
3035 1517 32 32
PpuMI rG'GwC_Cy
Cuts at: 321 321
Size: 4616
PstI C_TGCA'G
Cuts at: 652 1583 1663 2348 652
Size: 931 80 685 2920
Fragments arranged by size:
2920 931 685 80
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4616
PvuII CAG'CTG
Cuts at: 1347 2041 2401 1347
Size: 694 360 3562
Fragments arranged by size:
3562 694 360
RsrII CG'GwC_CG
Cuts at: 2811 2811
Size: 4616
SacI G_AGCT'C
Cuts at: 356 557 706 833 1244 1630 356
Size: 201 149 127 411 386 3342
Fragments arranged by size:
3342 411 386 201 149 127
SalI G'TCGA_C
Cuts at: 1653 1653
Size: 4616
SapI GCTCTTCn'nnn_
Cuts at: 452 838 2639 2849 452
Size: 386 1801 210 2219
Fragments arranged by size:
2219 1801 386 210
SbfI CC_TGCA'GG
Cuts at: 1663 1663
Size: 4616
ScaI AGT'ACT
Cuts at: 1408 4133 1408
Size: 2725 1891
SfcI C'TryA_G
Cuts at: 33 648 1523 1579 1659 2344 3337 3528 33
Size: 615 875 56 80 685 993 191 1121
Fragments arranged by size:
1121 993 875 685 615 191 80 56
SfiI GGCCn_nnn'nGGCC
Cuts at: 671 671
Size: 4616
SfoI GGC'GCC
Cuts at: 170 2048 2296 170
Size: 1878 248 2490
Fragments arranged by size:
2490 1878 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4616
SmaI CCC'GGG
Cuts at: 856 1638 856
Size: 782 3834
SmlI C'TyrA_G
Cuts at: 682 1006 1771 2686 3178 3440 682
Size: 324 765 915 492 262 1858
Fragments arranged by size:
1858 915 765 492 324 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4616
SphI G_CATG'C
Cuts at: 1669 2169 1669
Size: 500 4116
StuI AGG'CCT
Cuts at: 665 909 665
Size: 244 4372
StyI C'CwwG_G
Cuts at: 1034 1750 2104 1034
Size: 716 354 3546
Fragments arranged by size:
3546 716 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 247 1356 2199 2332 2791 3000 247
Size: 1109 843 133 459 209 1863
Fragments arranged by size:
1863 1109 843 459 209 133
TatI w'GTAC_w
Cuts at: 1406 4131 1406
Size: 2725 1891
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 714 746 4133 4165 714
Size: 32 3387 32 1165
Fragments arranged by size:
3387 1165 32 32
Tth111I GACn'n_nGTC
Cuts at: 2413 2413
Size: 4616
XbaI T'CTAG_A
Cuts at: 1647 1647
Size: 4616
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2111 2111
Size: 4616
XmaI C'CCGG_G
Cuts at: 854 1636 854
Size: 782 3834
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI BsgI BsmI BsmBI
BsrGI BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI